CN107505454A - Reaction system for detecting blood coagulation and platelet functions and application thereof - Google Patents
Reaction system for detecting blood coagulation and platelet functions and application thereof Download PDFInfo
- Publication number
- CN107505454A CN107505454A CN201710484300.5A CN201710484300A CN107505454A CN 107505454 A CN107505454 A CN 107505454A CN 201710484300 A CN201710484300 A CN 201710484300A CN 107505454 A CN107505454 A CN 107505454A
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- Prior art keywords
- coagulation
- reaction system
- platelet function
- reagent cup
- detects
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/4905—Determining clotting time of blood
Abstract
The invention discloses a reaction system for detecting blood coagulation and platelet functions, which comprises a probe, a reagent cup with a reagent cup cover, a stirring rod and a matched detection reagent. The reaction system reflects the blood coagulation effect and the platelet function of different blood coagulation processes according to the change of mechanical resistance to external force when the physical state of blood coagulation changes. Can be used for clinically monitoring blood coagulation and platelet functions in real time and predicting thrombus and bleeding risks. Simple operation and quick response, and can provide timely and accurate reference basis for clinic.
Description
Technical field
The invention belongs to technical field of medical instruments, be related to a kind of Coagulation and platelet function detection reaction system and
It is applied.
Background technology
Angiocardiopathy is the maximum potential killer of adult, only just has more than 70,000,000 people every year in the U.S. and suffers from heart
Disease, there are about 6,000,000 people to enter casualty department because of pectoralgia every year, wherein 2,500,000 patients are acute coronary syndrome
(ACS), the acute myocardial infarction patients (AMl) including 1,500,000,1,000,000 patients with unstable angina, and it is annual acute
The death rate of myocardial infarction has exceeded 500,000, that is to say, that it is total that the real AMl for threatening patients ' lives safety only accounts for chest pain patient
Several 1/4, and other chest pain patients need not make further treatment in hospital again, if chest pain patient can not be made a definite diagnosis in a short time
If whether suffering from fatal AMI, then what those non-AMI patient not only will be for no reason is stricken with anxiety and fear, and to undertake daily
Extra wholesale pharmaceuticals take and cost of hospitalization.Moreover, some AMI patients prolong because diagnosis cannot be treated in time not in time
The state of an illness is even dead by mistake.
In recent years, due to the development of current new and high technology and the progress of medical science, and efficiently allegro work side
Formula so that there is laboratory apparatus miniaturization, simple operation, report that the POCT of result just-in-time increasingly receives the green grass or young crops of people
Look at.
Laboratory is sent to after most coagulation analysis blood samplings to be detected by automation Blood coagulation instrument, the turnaround time
It is long, censorship and the improper easily influence specimen quality of storage.POCT quasi-instruments arise at the historic moment, and activated clotting time (ACT) is wherein
The most sufficient index of application, mainly used in clinical department, a drop peripheral blood is the substantially effect that can obtain anticoagulant heparin.State
The viscoplasticity of thrombus is detected using the method for ultrasound detection outside, in addition to predicting bleeding, platelet function is also used for and comments
Estimate.The country, which does not carry out also, at present tests oneself.
The present invention is provided commonly for blood coagulation and blood platelet work(using probe, reagent cup lid, reagent cup and the part of stirring rod four
The reaction system that can be detected, whether this has thrombotic dangerous and blood platelet relevant disease diagnosis to early detection and controls
Treatment has great significance.
The content of the invention
The purpose of the present invention is the shortcomings that overcoming prior art and deficiency, there is provided a kind of small volume, tight ness rating are high, it is moistureproof with
And the reaction system of the Coagulation and platelet function detection of the advantages that simple to operate.
To achieve the above object, the invention discloses following technology contents：
A kind of reaction system of Coagulation and platelet function detection, it is characterised in that including probe, reagent cup lid, reagent
Cup, stirring rod.This four be partially bonded together form Coagulation and platelet function detection reaction system.
The reaction system of Coagulation and platelet function detection of the present invention, reagent cup and reagent cup lid are medical high-strength degree
Transparent PP, and antistatic agent is with the addition of, it is to be molded by precise injection mould.Reagent cup is combined closely simultaneously with reagent cup lid
And without electrostatic interference.
The reaction system of Coagulation and platelet function of the present invention detection, reagent cup height of contour are 15-20mm, rim of a cup
A diameter of 10mm-15mm, bottom of a cup a diameter of 8mm-13mm, volume 340-360mm3。
The reaction system of Coagulation and platelet function detection of the present invention, a diameter of 15-20mm of reagent cup lid, both ends are set
Arc is calculated as, conveniently can pick up and uncap.There is a groove at cup lid center, for aiding in installing probe.
The reaction system of Coagulation and platelet function of the present invention detection, the material of probe are polypropylene (PP), length
It is flexible, highly polished for 21-26mm, external diameter 4-9mm, internal diameter 3-8mm.
Stirring rod is contained in the reaction system of Coagulation and platelet function detection of the present invention, inside, and the stirring rod has
Magnetic, a diameter of 1-6mm, length 5mm-10mm, length should be less than bottom of a cup diameter.
The reaction system of Coagulation and platelet function detection of the present invention detects applied to Coagulation and platelet function.
The reaction system of Coagulation and platelet function detection of the present invention can add coagulation activation agent group in reagent cup
Into Coagulation test system, detected applied to Coagulation and platelet function.
Brief description of the drawings：
Fig. 1 is human body fluid detection architecture overall diagram (the reaction system overall diagram of Coagulation and platelet function detection)
2. it is reagent cup lid 1. being probe, is 3. reagent cup, is 4. stirring rod.
Fig. 2 is human body fluid detection architecture each several part figure (the reaction system each several part figure of Coagulation and platelet function detection)
2. it is reagent cup lid 1. being probe, is 3. reagent cup, is 4. stirring rod.
Fig. 3 is the Coagulation and platelet function detection figure of anti-freezing blood sample
What the smoothed curve started in figure represented is ACT change, i.e. activated coagulation time；The slope of curve of rising
Represent CR change, i.e. blood coagulation speed；Last curve peak cartographic represenation of area PF, i.e. platelet function.
Fig. 4 is the Coagulation and platelet function detection figure of nature whole blood sample
What the smoothed curve started in figure represented is ACT change, i.e. activated coagulation time；The slope of curve of rising
Represent CR change, i.e. blood coagulation speed；Last curve peak cartographic represenation of area PF, i.e. platelet function.
Embodiment
The present invention is absolutely proved below according to specific embodiment.
Embodiment
Embodiment 1 is used for the reaction system of Coagulation and platelet function detection
Reaction system composition and particular content such as following table for Coagulation and platelet function detection：
The application of the reaction system of the Coagulation and platelet function of embodiment 2 detection
Prepare 1. examining
1) probe prepares
1. reaction probe is inserted into reagent cup lid central recess, hold reagent cup and take out, and the probe other end is carefully inserted
Enter instrument probe installation axis part.Reagent cup is pulled out, probe is fixed on installation axle in the heart.
2. open reagent cup lid with thumb and forefinger.The reagent cup of opening is gently inserted in reagent cup fixed seat, preheated
No less than 30 seconds.
2. add sample
1) natural whole blood sample
The blood sample that syringe gathers is injected into preheated reagent cup.It should remove syringe needle when injecting blood sample,
Front end of the syringe needle about 0.5mL blood samples are discarded, afterwards injected remaining blood sample in reagent cup until horizontal plane is slightly below reagent cup
Inner ring, volume is about 360 μ L.
2) anti-freezing blood sample
1. take out 500 μ L anti-freezing blood samples with pipettor to be transferred in EP pipes.
2. fastening EP is managed and overturn 3 times, make blood wall built-up in pipe.
3. adding 20 μ L0.25M calcium chloride into EP pipes, overturn 3 times.(to ensure that result is accurate, the step please be shorten
Operating time, it is proposed that adjust pipettor to 360 μ L before calcium chloride is added.)
4. taking out 360 μ L sample of blood using pipettor, reagent cup is added along reagent wall of cup.
3. detection
After sample is added into reagent cup, it can be rotated automatically by lower switch, magnetic stirring bar immediately.
After the completion of mixing, analyzer meeting Pop-up message window, prompt to close Cang Gai, start to detect.
4. result calculates
In test process, analyzer can send " ticking " prompt tone, prompt activation whole blood coagulation time (ACT), blood coagulation speed
(CR) and platelet function parameter (PF) generation, and record result automatically.
5. result
Drawn a conclusion by above-mentioned experiment：During system detection Coagulation and platelet function of the present invention, its ACT value is effective
In the range of, CR values difference is smaller, and PF values are equal>1.0 and stably.It can be seen that system of the present invention can make reagent keep it
Stability.
The application of the supporting detection reagent of reaction system of the Coagulation and platelet function of embodiment 3 detection
Activator of the bead as Coagulation test is contained in the reaction system of Coagulation and platelet function detection, then is divided
It is other that natural whole blood sample and anti-freezing blood sample are detected.
Prepare 1. examining
2) reagent and probe prepare
1. keep reagent cup/lid upward, the quick knocking reagent cup on hard thing surface, to remove reagent cup inwall and lid
Bead powder on son sinks to the bottom it.
2. reaction probe is inserted into reagent cup lid central recess, hold reagent cup and take out, and the probe other end is carefully inserted
Enter instrument probe installation axis part.Reagent cup is pulled out, probe is fixed on installation axle in the heart.
3. open reagent cup lid with thumb and forefinger.The reagent cup of opening is gently inserted in reagent cup fixed seat, preheated
No less than 30 seconds.
2. add sample
3) natural whole blood sample
The blood sample that syringe gathers is injected into preheated reagent cup.It should remove syringe needle when injecting blood sample,
Front end of the syringe needle about 0.5mL blood samples are discarded, afterwards injected remaining blood sample in reagent cup until horizontal plane is slightly below reagent cup
Inner ring, volume is about 360 μ L.
4) anti-freezing blood sample
1. take out 500 μ L anti-freezing blood samples with pipettor to be transferred in EP pipes.
2. fastening EP is managed and overturn 3 times, make blood wall built-up in pipe.
3. adding 20 μ L0.25M calcium chloride into EP pipes, overturn 3 times.(to ensure that result is accurate, the step please be shorten
Operating time, it is proposed that adjust pipettor to 360 μ L before calcium chloride is added.)
4. taking out 360 μ L sample of blood using pipettor, reagent cup is added along reagent wall of cup.
3. detection
After sample is added into reagent cup, it can be rotated automatically by lower switch, magnetic stirring bar immediately.
After the completion of mixing, analyzer meeting Pop-up message window, prompt to close Cang Gai, start to detect.
4. result calculates
In test process, analyzer can send " ticking " prompt tone, prompt activation whole blood coagulation time (ACT), blood coagulation speed
(CR) and platelet function parameter (PF) generation, and record result automatically.
5. result
What the smoothed curve started in figure represented is ACT change, i.e. activated coagulation time；The slope of curve of rising
Represent CR change, i.e. blood coagulation speed；Last curve peak cartographic represenation of area PF, i.e. platelet function.
Drawn a conclusion by above-mentioned experiment：During system detection Coagulation and platelet function of the present invention, its ACT value is effective
In the range of, CR values difference is smaller, and PF values are equal>1.0 and stably.It can be seen that system of the present invention can make reagent keep it
Stability.
Claims (8)
- A kind of 1. reaction system of Coagulation and platelet function detection, it is characterised in that including probe, reagent cup lid, reagent cup, Stirring rod, this four be partially bonded together form Coagulation and platelet function detection reaction system.
- 2. the reaction system that Coagulation and platelet function described in claim 1 detects, reagent cup and reagent cup lid are medical high-strength Transparent PP is spent, and with the addition of antistatic agent, is molded by precise injection mould, reagent cup is combined closely simultaneously with reagent cup lid And without electrostatic interference.
- 3. the reaction system that Coagulation and platelet function described in claim 1 detects, reagent cup height of contour are 15-20mm, cup Mouth a diameter of 10mm-15mm, bottom of a cup a diameter of 8mm-13mm, volume 340-360mm3。
- 4. the reaction system that Coagulation and platelet function described in claim 1 detects, a diameter of 15-20mm of reagent cup lid, both ends Arc is designed as, conveniently can pick up and uncap, there is a groove at cup lid center, for aiding in installing probe.
- 5. the reaction system that Coagulation and platelet function described in claim 1 detects, the material of probe is polypropylene（PP）, length For 21-26mm, external diameter 4-9mm, internal diameter 3-8mm.
- 6. stirring rod is contained in the reaction system that Coagulation and platelet function described in claim 1 detects, inside, the stirring rod has Magnetic, a diameter of 1-6mm, length 5mm-10mm, length should be less than bottom of a cup diameter.
- 7. the reaction system that Coagulation and platelet function described in claim 1 detects detects applied to Coagulation and platelet function.
- 8. the reaction system that Coagulation and platelet function described in claim 1 detects, can add coagulation activation agent in reagent cup Coagulation test system is formed, is detected applied to Coagulation and platelet function.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710484300.5A CN107505454A (en) | 2017-06-23 | 2017-06-23 | Reaction system for detecting blood coagulation and platelet functions and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710484300.5A CN107505454A (en) | 2017-06-23 | 2017-06-23 | Reaction system for detecting blood coagulation and platelet functions and application thereof |
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| CN107505454A true CN107505454A (en) | 2017-12-22 |
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| CN201710484300.5A Pending CN107505454A (en) | 2017-06-23 | 2017-06-23 | Reaction system for detecting blood coagulation and platelet functions and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112941143A (en) * | 2021-01-28 | 2021-06-11 | 南京可诺医疗技术有限公司 | Simple and convenient reagent for detecting blood coagulation and platelet functions and application thereof |
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| CN104458503A (en) * | 2014-12-12 | 2015-03-25 | 广州阳普医疗科技股份有限公司 | Blood coagulation detection instrument |
| CN104545943A (en) * | 2014-12-06 | 2015-04-29 | 深圳市共创百业科技开发有限公司 | Coagulometer and medical system of network hospital for preventing thrombus |
| CN104730260A (en) * | 2015-04-14 | 2015-06-24 | 中国科学院苏州生物医学工程技术研究所 | Portable mobile blood clotting analyzing system and blood clotting analyzing method |
| WO2016046565A2 (en) * | 2014-09-25 | 2016-03-31 | Benson Viscometers Ltd | Apparatus for monitoring blood coagulation |
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| CN1791800A (en) * | 2003-03-21 | 2006-06-21 | 赫默维神有限责任公司 | Erythrocyte sedimentation rate (ESR) test measurement instrument of unitary design and method of using the same |
| WO2016046565A2 (en) * | 2014-09-25 | 2016-03-31 | Benson Viscometers Ltd | Apparatus for monitoring blood coagulation |
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| CN104458503A (en) * | 2014-12-12 | 2015-03-25 | 广州阳普医疗科技股份有限公司 | Blood coagulation detection instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112941143A (en) * | 2021-01-28 | 2021-06-11 | 南京可诺医疗技术有限公司 | Simple and convenient reagent for detecting blood coagulation and platelet functions and application thereof |
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Application publication date: 20171222 |
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