CN107505310B - Bio-energy shifts luminescence analyzer - Google Patents
Bio-energy shifts luminescence analyzer Download PDFInfo
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- CN107505310B CN107505310B CN201710695840.8A CN201710695840A CN107505310B CN 107505310 B CN107505310 B CN 107505310B CN 201710695840 A CN201710695840 A CN 201710695840A CN 107505310 B CN107505310 B CN 107505310B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
- G01N21/763—Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The present invention relates to field of biological detection, provide a kind of bio-energy transfer luminescence analyzer.It includes: outer housing that bio-energy, which shifts luminescence analyzer,；And it is contained in the enzyme mark cup assembly in outer housing, drive transmission assembly and filter disc receiver assembly, filter disc receiver assembly includes filter disc, receiver and the movable ram being arranged in below filter disc, movable ram includes cylindrical body, in the top of cylindrical body horizontal-extending edge outward, and the tapered portion formed in the bottom end of cylindrical body, and movable ram can move up and down, when enzyme mark cup contacts and when further towards movable ram, tapered portion is jacked up by the rim of a cup of enzyme mark cup, when the rim of a cup of enzyme mark cup moves to the underface of movable ram, movable ram moves downward to be pressed into enzyme mark cup in through-holes due to gravity.According to the present invention, meet and be protected from light requirement using the bio-energy transfer luminescence analyzer of the testing principle of photon, so that structure is simple and convenient to operate.
Description
Technical field
The present invention relates to field of biological detection, shift luminescence analyzer more particularly to a kind of bio-energy.
Background technique
Microplate reader is the special instrument of enzyme-linked immunosorbent assay, it is widely used in biomedical research and clinical diagnosis
In, for carrying out detection and quantitative analysis immune response.Microplate reader working principle is exactly the light wave of illuminator sending by filtering
Piece or monochromator become the light of a branch of specific wavelength, the testing sample solution into the micropore of ELISA Plate.The specific wavelength
Light a part is absorbed by sample solution, and another part then penetrates sample solution and is irradiated on photoelectric detector, and photoelectric detector will
This testing sample solution is different and optical signal of different strengths and weaknesses is converted into corresponding electric signal, and electric signal is put big, right before menstruation
Microprocessor is sent into after the signal processings such as number amplification, analog-to-digital conversion and carries out data processing and calculating, finally by display or printing
Machine shows result.
However, existing microplate reader needs so structure is complicated, and to need to calibrate the position of fluorescence using external light source
It sets.It is then desired to a kind of sample solution autofluorescence and do not have to external light source detected bio-energy transfer luminesceence analysis
Instrument: bio-energy transfer luminescence analyzer can solve or at least mitigate some drawbacks in the presence of the above-mentioned prior art or
Person's whole disadvantage.
Summary of the invention
Technical problem to be solved by the present invention lies in: provide one kind aim to solve the problem that or at least mitigate it is above-mentioned in the prior art
The bio-energy of existing disadvantage shifts luminescence analyzer.
The technical solution adopted in the present invention is as described below.
According to an aspect of the present invention, a kind of bio-energy transfer luminescence analyzer is provided, wherein the bio-energy turns
Moving luminescence analyzer includes:
Outer housing；And
Enzyme mark cup assembly, driving transmission assembly and the filter disc receiver assembly being contained in the outer housing,
Wherein, the enzyme mark cup assembly is driven and is transmitted to the filter disc from initial position by the driving transmission assembly
At receiver assembly,
The filter disc receiver assembly include filter disc, receiver and be arranged in below the filter disc can dynamic pressure
Head, and wherein,
The filter disc can rotate, for the cylindrical shape bottom of with and with cylindric Pan Bi and circular discs bottom, described
Pan Bi is provided with circular hole, and optical filter is housed in the circular hole,
The movable ram include cylindrical body, the top of cylindrical body outward horizontal-extending edge and
In the tapered portion that the bottom end of cylindrical body is formed, and the movable ram can move up and down, when the enzyme mark cup contacts
And when further towards the movable ram, the tapered portion is jacked up by the rim of a cup of the enzyme mark cup, when the cup of the enzyme mark cup
When mouthful moving to the underface of the movable ram, the movable ram due to gravity move downward in the through hole from
And it is pressed into the enzyme mark cup.
Preferably, the filter disc receiver assembly further includes fixed block and receiver holder, the receiver and described
Receiver holder is respectively positioned on the inside of the filter disc, and the fixed block is located at the lowest part along the vertical direction of the filter disc
On the outside of the disk wall divided and including through-hole, the receiver holder is fixed on the fixed block, and the movable ram is described logical
It moves up and down in hole.
Preferably, the Pan Bi is circumferentially provided with multiple circular holes with uniform intervals, is respectively provided in the multiple circular hole
Different types of optical filter.
Preferably, the enzyme mark cup assembly includes enzyme mark cup base, enzyme mark cup and opening-closing device, the enzyme mark cup setting
On the enzyme mark cup base, the opening-closing device includes the first opening and closing part and the second opening and closing part, the first opening and closing part and the
The first end of two opening and closing parts is pivotally connected to the enzyme mark cup base so that the first opening and closing part and second
Opening and closing part can open and drawing, and the mutually opposed surface of the first opening and closing part and the second opening and closing part is provided with and the enzyme
Mark the concave surface portion of the form fit of cup.
Preferably, the opening-closing device further includes elastic component, and elastic component setting is in the first opening and closing part and described
So as to by the first opening and closing part and the second opening and closing part drawing on second opening and closing part.
Preferably, the bio-energy transfer luminescence analyzer further includes wedge piece, and the wedge piece is fixed at institute
It states on outer housing, it is vertical to be respectively arranged at the lower surface near the second end of the first opening and closing part and the second opening and closing part
The pin extended downwardly, the pin are slided along two sides of the wedge piece.
Preferably, the outer housing includes first shell portion and second shell portion, the first shell portion and described second
Edge joint position between housing section is provided with one or more sets joinery and its constructions.
Preferably, the first shell portion and the second shell portion are hollow, and the second shell portion includes water
Left the half of the horizontal extension in the second shell portion is arranged in flat extension and vertical extension, the first shell portion
On the top surface in portion, the upper side in the downside in the first shell portion and the second shell portion, which is equipped with, makes the first shell
The opening in portion and second shell portion perforation.
Preferably, it is provided with recess portion on the upper side of the right side of the horizontal extension in the second shell portion, institute
The bottom for stating recess portion is equipped with opening, is additionally provided with flip lid corresponding to the recess portion in the second shell portion, under the flip lid
Surface is formed with downward projection of protrusion, and the flip lid is pivotally installed in the second shell portion using shaft,
It is described so that the flip lid is closing the detent position of the recess portion and making to move between the open open position of the recess portion
The periphery of recess portion is provided with joinery and its construction, and the recess portion is also corresponded to around the protrusion and is provided with joinery and its construction.
One or more embodiment according to the present invention, can be realized beneficial effect as described below.
According to the present invention, the movable ram includes cylindrical body, in the outside horizontal extension in the top of cylindrical body
Edge and cylindrical body bottom end formed tapered portion, and the movable ram in the through hole up and down fortune
Dynamic, when the enzyme mark cup contacts and when further towards the movable ram, the tapered portion is by the rim of a cup top of the enzyme mark cup
It rises, when the rim of a cup of the enzyme mark cup moves to the underface of the movable ram, the movable ram is existed due to gravity
It moves downward to be pressed into the enzyme mark cup in the through-hole.Thus, the movable ram is pressed into the enzyme mark cup and to realize
Light-shading effect.This, which can satisfy, is protected from light requirement using the bio-energy transfer luminescence analyzer of the testing principle of photon, from
And realize the purpose that structure is simple and convenient to operate.The shortcomings that this has been alleviated or avoided in the prior art.
In some embodiments, the Pan Bi is circumferentially provided with multiple circular holes, the multiple circular hole with uniform intervals
In be respectively provided with different types of optical filter.Six kind different waves can be analyzed the sample solution in each enzyme mark cup in this way.
In other embodiments, since the first opening and closing part, the second opening and closing part and enzyme mark cup base are constituted and blocked
Structure, can block light, effectively avoid influence of the ambient to each enzyme mark cup aperture, to avoid in enzyme mark cup
Sample solution testing result influence；It is also possible that there is no interacting for spontaneous light between every two enzyme mark cup.
Light-shading effect is thus furthermore achieved.
In some other embodiments, the outer housing includes first shell portion and second shell portion, the first shell
Edge joint position between portion and the second shell portion is provided with one or more sets joinery and its constructions, thus further realizes and be protected from light
Effect.
In still further embodiments, the periphery of the recess portion is provided with joinery and its construction, also corresponding around the protrusion
The recess portion is provided with joinery and its construction, thus further realizes light-shading effect.
Detailed description of the invention
It with reference to the accompanying drawings will be to the feature, advantage of invention and the technical of illustrative embodiments and industrial meaning
Justice is described, and in the accompanying drawings, identical appended drawing reference indicates identical element, and wherein:
Fig. 1 is the schematic perspective view of the bio-energy transfer luminescence analyzer of illustrative embodiments according to the present invention,
Wherein flip lid fully opens；
Fig. 2 is that showing for illustrative embodiments is renovated when going to certain angle before and after Fig. 1 according to the present invention
The side sectional view of resulting bio-energy transfer luminescence analyzer is cut in direction from second shell portion, and in order to clearly rise
See, all parts inside outer housing are omitted；
Fig. 3 is the main view of the bio-energy transfer luminescence analyzer of illustrative embodiments according to the present invention；
Fig. 4 be according to the present invention illustrative embodiments show flip lid closing when along Fig. 3 A-A in the front-back direction
Line cuts the side sectional view of resulting bio-energy transfer luminescence analyzer from second shell portion, and for the sake of clarity,
The various components inside outer housing are omitted；
Fig. 5 is the portion inside the outer housing of the bio-energy transfer luminescence analyzer of illustrative embodiments according to the present invention
Enzyme mark cup assembly and driving transmission assembly is shown in the side view of subassembly；
Fig. 6 is the top view of the various components of Fig. 5, and wherein the opening-closing device of enzyme mark cup assembly opens；
Fig. 7 is the face upwarding stereogram of the various components of Fig. 5；
Fig. 8 is the top view of a part of bio-energy transfer luminescence analyzer, and enzyme mark cup assembly is shown, and
The opening-closing device of enzyme mark cup assembly is closed；
Fig. 9 is a part of the bio-energy transfer luminescence analyzer of Fig. 8 along the resulting cross-sectional view of line A-A cutting, wherein
Show the heating sheet being attached to below enzyme mark cup base；
Figure 10 is inside the outer housing of bio-energy transfer luminescence analyzer of illustrative embodiments according to the present invention
Filter disc receiver assembly and part enzyme mark cup assembly is shown in the main view of members；
Figure 11 is the perspective view of the members inside the outer housing of the bio-energy transfer luminescence analyzer of Figure 10；
Figure 12 is the top view of the members inside the outer housing of the bio-energy transfer luminescence analyzer of Figure 10；
Figure 13 is the plastic inner lining between optical filter and filter disc of the bio-energy transfer luminescence analyzer of Figure 10
Perspective view；
Figure 14 is the solid for the receiver holder of the bio-energy transfer luminescence analyzer of Figure 10 being located at below receiver
Figure；
Figure 15 is the perspective view for the fixed block of the bio-energy transfer luminescence analyzer of Figure 10 being located at below filter disc；
Figure 16 a and 16b be Figure 10 bio-energy transfer luminescence analyzer in the through-hole of fixed block can on move down
The perspective view of dynamic movable ram；
Figure 17 is receiver, the receiver of the bio-energy transfer luminescence analyzer of illustrative embodiments according to the present invention
The perspective view that bracket, fixed block and movable ram are assembled together；And
Figure 18 is the resulting receiver of line B-B cutting along Figure 17, receiver holder, fixed block and movable ram assembly
Cross-sectional view together.
Specific embodiment
Exemplary embodiment of the invention is described in detail with reference to the accompanying drawings.Description to illustrative embodiments
It merely for the sake of demonstration purpose, and is definitely not to the present invention and its application or the limitation of usage.Moreover, in figure each component size
It is also only schematically, not exactly correspond to actual product with ratio.
Bio-energy transfer luminescence analyzer of the invention is a Table top type small-size laboratory instrument, is supported for enzyme mark
Cup carries out reaction test, can analyze six kinds of different wave lengths to the sample solution in each enzyme mark cup.Cardinal principle is as follows: changing
It learns, testing concentration and the chemiluminescence intensity of system linear quantitative relationship under certain condition in detection architecture, biological energy source
Amount transfer luminescence analyzer determines determinand content by the detection to system different wave length chemiluminescence intensity.Specifically
Ground, bio-energy transfer luminescence analyzer pass through the reading to certain wavelength autofluorescence photon numbers of sample solution in enzyme mark cup
It takes, detects the ingredient of the sample solution.More specific information, can with reference to the applicant application No. is 201410854006.5
Patent of invention content.
Staff opens flip lid, will fill sample solution after making bio-energy transfer luminescence analyzer be powered
After enzyme mark cup is put into the correspondence hole location in right brackets.It is operated according to the prompt at interface on touch screen.It is interior in detection process
Portion's motivation structure can send enzyme mark cup to sensor receiving port, and sensor most multipotency reads six kinds of differences of eight kinds of reagents respectively
The number of photons of wavelength.
It include the bio-energy transfer luminescence analyzer of illustrative embodiments according to the present invention referring to Fig. 1-4 pairs
External structure is illustrated.Fig. 1 is the signal of the bio-energy transfer luminescence analyzer of illustrative embodiments according to the present invention
Property perspective view, wherein flip lid fully open.Fig. 2 is that the flip lid that shows of illustrative embodiments according to the present invention is being gone to centainly
Cut the side, sectional of resulting bio-energy transfer luminescence analyzer when angle from second shell portion along the front-rear direction of Fig. 1
Figure, and for the sake of clarity, all parts inside outer housing are omitted.Fig. 3 is illustrative embodiments according to the present invention
Bio-energy transfer luminescence analyzer top view.Fig. 4 is that the flip lid that shows of illustrative embodiments according to the present invention exists
Cut the side of resulting bio-energy transfer luminescence analyzer when closing from second shell portion along the line A-A in the front-back direction of Fig. 3
Face cross-sectional view, and for the sake of clarity, the various components inside outer housing are omitted.
As shown in Figs 1-4, the bio-energy transfer luminescence analyzer of illustrative embodiments includes shell according to the present invention
Body 1.Outer housing 1 includes first shell portion 11 and second shell portion 12.First shell portion 11 and second shell portion 12 are hollow
's.Second shell portion 12 includes horizontal extension and vertical extension, specifically, from Fig. 1 left sides, second shell portion 12
It is L-shaped.First shell portion 11 is arranged on the top surface of the left side of the horizontal extension in second shell portion 12.First shell portion 11
Downside and the upper side in second shell portion 12 be open, and first shell portion 11 and second shell portion 12 pass through these and are open
Perforation.It is provided with display operation screen on the leading flank in first shell portion 11, luminesceence analysis is shifted to bio-energy for user
Instrument carries out required operation.Recess portion 1211 is provided on the upper side of the right side of the horizontal extension in second shell portion 12.It is recessed
The bottom in portion 1211 is equipped with opening.Flip lid 121 is additionally provided with corresponding to recess portion 1211 on the upper side in second shell portion 12.Flip lid
121 are installed in second shell portion 12 using shaft, and renovating 121 in this way can pivotally move, so as to close
The detent position of recess portion 1211 and make to move between the open open position of recess portion 1211.When needing test sample solution,
Staff opens flip lid 121, and the enzyme mark cup equipped with sample solution is then put into second shell portion 12 by recess portion 1211
In the accommodating space of horizontal extension.
The upper surface of flip lid 121 is flat surface, and the lower surface for renovating 121 is formed with downward projection of protrusion 1221.This is convex
Portion 1221 and flip lid 121 are rectangle, but the rectangle of the squareness ratio of protrusion 1221 flip lid 121 wants small.Due to molten to sample
Liquid carry out detection need it is good be protected from light, thus 1211 periphery of recess portion is provided with two sets of joinery and its constructions.It is set with 1211 periphery of recess portion
The two sets of joinery and its constructions set accordingly, are renovated and are also provided with two sets of joinery and its constructions around 121 protrusion 1221, in this way, when flip lid 121
When closing recess portion 1211, two sets of Tenon knots of the two sets of joinery and its constructions and 1211 periphery of recess portion around 121 protrusion 1221 are renovated
Structure is fitted close, and is protected from light requirement to realize.It should be noted that two sets of above-mentioned joinery and its constructions are only exemplary,
It can according to need a set of or more than two sets the joinery and its construction of setting.Also, it is preferred that between the component part of all outer housings
Edge joint position between edge joint position and each component part and other component engaged therewith is designed at least a set of joinery and its construction.This
A little joinery and its constructions are to guarantee that sensor only only has the shape of 10 digit photons to be protected from light key in initial reading.Moreover, further
Preferably, black paste paint is all sprayed in the inside of all outer housings, to guarantee that white light will not penetrate.
Preferably, the upper side of the right side of the horizontal extension in second shell portion 12 is slotted at the both ends of shaft
1223, flip lid 121 is simply disassembled when rotation is to 45 °.Preferably, fixation is additionally provided on flip lid 121
Part 1222, be provided in second shell portion 12 with the matched fixation hole 1212 of fixing piece, can be firmly with to renovate 121
The matching of two housing sections 12.
Certainly, the setting position of the shape of above-mentioned outer housing 1, display operation screen and recess portion etc. is only exemplary, this
Field those of ordinary skill is it is understood that the setting position of shape, display operation screen and recess portion of outer housing 1 etc. can be with
It is changed as needed.
Luminescence analyzer is shifted referring to the bio-energy that Fig. 5-18 pairs include illustrative embodiments according to the present invention
The various components being contained in inside outer housing be illustrated.Fig. 5 is the bio-energy of illustrative embodiments according to the present invention
Enzyme mark cup assembly and driving transmission group is shown in the side view for shifting the members inside the outer housing of luminescence analyzer
Part.Fig. 6 is the top view of the various components of Fig. 5, and wherein the opening-closing device of enzyme mark cup assembly opens.Fig. 7 is the various components of Fig. 5
Face upwarding stereogram.Fig. 8 is the top view of a part of bio-energy transfer luminescence analyzer, and enzyme mark cup group is shown
Part, and the opening-closing device closure of enzyme mark cup assembly.Fig. 9 is a part of the bio-energy transfer luminescence analyzer of Fig. 8 along A-A
The heating sheet being attached to below enzyme mark cup base is shown in the resulting cross-sectional view of wire cutting.Figure 10 is example according to the present invention
Property embodiment bio-energy transfer luminescence analyzer outer housing inside members main view, filter is shown
CD receiver assembly and part enzyme mark cup assembly.Figure 11 is inside the outer housing of bio-energy transfer luminescence analyzer of Figure 10
Members perspective view.Figure 12 is the members inside the outer housing of the bio-energy transfer luminescence analyzer of Figure 10
Top view.Figure 13 is the plastic inner lining between optical filter and filter disc of the bio-energy transfer luminescence analyzer of Figure 10
Perspective view.Figure 14 is the solid for the receiver holder of the bio-energy transfer luminescence analyzer of Figure 10 being located at below receiver
Figure.Figure 15 is the perspective view for the fixed block of the bio-energy transfer luminescence analyzer of Figure 10 being located at below filter disc.Figure 16 a and
16b is the vertical of the movable ram that can move up and down in the through-hole of fixed block of the bio-energy transfer luminescence analyzer of Figure 10
Body figure.Figure 17 is the receiver of the bio-energy transfer luminescence analyzer of illustrative embodiments according to the present invention, receiver branch
The perspective view that frame, fixed block and movable ram are assembled together.Figure 18 be along Figure 17 the resulting receiver of line B-B cutting,
The cross-sectional view that receiver holder, fixed block and movable ram are assembled together.
Bio-energy transfer luminescence analyzer includes enzyme mark cup assembly 2, the driving transmission assembly being contained in inside outer housing
3, filter disc receiver assembly 4 etc..Entire enzyme mark cup assembly 2 moves to filter disc under the driving of driving transmission assembly 3 and receives
At device assembly 4, the sample solution in enzyme mark cup is tested and analyzed by filter disc receiver assembly 4.
Enzyme mark cup assembly 2 includes enzyme mark cup base 21, enzyme mark cup 22 and opening-closing device 23.It is arranged on enzyme mark cup base 21
There are multiple enzyme mark cups 22, in the embodiment of Fig. 6 of the present invention, including eight enzyme mark cups 22.Opening-closing device 23 includes first
Component, the second opening and closing part and spring.As shown in fig. 6, one end of the first opening and closing part and the second opening and closing part (is left in Fig. 6
End) it is as pivotally connected on enzyme mark cup base 21, as shown in fig. 6, the second end of the first opening and closing part and the second opening and closing part
(being right end in Fig. 6) opens.The mutually opposed surface of first opening and closing part and the second opening and closing part is both provided with and enzyme mark
The concave surface portion of the form fit of cup 22, as shown in figure 8, when the first opening and closing part and the second opening and closing part are closed, first
Component and the second opening and closing part are arranged in parallel with a fixed spacing, and each concave surface of the first opening and closing part and the second opening and closing part
Portion clamps eight enzyme mark cups 22.When staff opens flip lid 121 and places enzyme mark cup 22, the first opening and closing part and the second opening and closing
Part is in position as shown in FIG. 6.Optical filtering disk receiving group is moved to left side shown in Fig. 6 in 2 entirety of enzyme mark cup assembly
When 4 underface of part, the first opening and closing part and the second opening and closing part are in position as shown in Figure 8.First opening and closing part, the second opening and closing part
And enzyme mark cup base constitutes barrier structure, can block to light, effectively avoids ambient to each enzyme mark cup aperture
Influence, to avoid the influence of the testing result to the sample solution in enzyme mark cup；It is also possible that every two enzyme mark cup it
Between interacting without spontaneous light.The detection that this carries out bio-energy transfer luminescence analyzer is very crucial.
The opening and closing principle of first opening and closing part and the second opening and closing part is described referring to Fig. 6-8.As shown in fig. 7, first
The pin 231 extended straight down, and are respectively arranged at lower surface near the second end of component and the second opening and closing part
Two 231 positional symmetries of pin being arranged on one opening and closing part and the second opening and closing part.Bio-energy shifts luminescence analyzer
Wedge piece 6, wedge piece 6 are fixed on outer housing 1.It is provided on the outer surface of first opening and closing part and the second opening and closing part
The both ends of cylinder 232, back-moving spring (not shown) are arranged on two cylinders 232.Enzyme mark cup assembly 2 is in driving transmission assembly 3
Under the action of left and right directions in Fig. 6 when moving, two pins 231 are slided along two sides of wedge piece 6.As shown in fig. 7,
When enzyme mark cup assembly 2 is moved to the left side of Fig. 7, two pins 231 along wedge piece 6 left slide from two sides to Fig. 7,
The distance between two sides of wedge piece 6 are shorter and shorter, between two pins 231 also because back-moving spring effect and make away from
It is shorter and shorter from correspondingly.When enzyme mark cup assembly 2 to move on the left of Fig. 7 exited completely from wedge piece 6 when, the first opening and closing part
And second opening and closing part be just tightened up spring straining be closed completely.When enzyme mark cup assembly 2 is from the first opening and closing part and second
The position that component is closed completely is moved to the right when two pins 231 contact wedge piece 6, and two pins 231 are pushed up by wedge piece 6
It opens, that is, the first opening and closing part and the second opening and closing part overcome the tightening force of back-moving spring and open as shown in fig. 6.With enzyme
The mark continuation of cup assembly 2 moves to the right, and the angle that the first opening and closing part and the second opening and closing part open is also increasing.In this way, In
Staff takes the position of enzyme mark cup, and the opening angle that the first opening and closing part and the second opening and closing part open allows to easily
It takes enzyme mark cup.It should be noted that one of ordinary skill in the art will appreciate that cylinder 232 and back-moving spring are set
Seated position can according to need setting, as long as above-mentioned function may be implemented.Moreover, the first opening and closing part and the second opening and closing part
Can also be by the other modes drawing except back-moving spring, such as resilient brace etc.；First opening and closing part and the second opening and closing part
Open and can also be realized by the other modes except pin and wedge piece, as long as the purpose of the present invention may be implemented.
Preferably, as shown in figure 9, heating sheet 9 can be posted below enzyme mark cup base 21, and such heating sheet can basis
Sample solution is heated to 60 DEG C -70 DEG C of temperature by experiment testing requirements.
Driving transmission assembly 3 is for being driven and being transmitted to entire enzyme mark cup assembly 2.As shown in fig. 6, driving transmission
Component 3 includes motor 31, synchronous belt 32, synchronous belt jockey pulley 33 and guide rail 34 etc..Synchronous belt 32 is arranged around synchronous belt jockey pulley 33,
Motor 31 drives the entire side-to-side movement on guide rail 34 together of enzyme mark cup assembly 2, so that realizing makes enzyme mark cup assembly 2 from recess portion
Position at 1211 moves to the position at filter disc receiver assembly 4, to be detected one by one to eight enzyme mark cups, thus
Meet that speed is fast, requirement with high accuracy.
Filter disc receiver assembly 4 is located at the inside in first shell portion 11 in Fig. 1.Below with reference to Figure 10-16a, 16b to filter
CD receiver assembly 4 is described in detail.As shown, filter disc receiver assembly 4 includes filter disc 41 and receiver
42.Filter disc 41 is the cylindrical shape bottom of with, has cylindric Pan Bi and circular discs bottom.Filter disc 41 is preferably black by 6063 oxygen
Aluminium alloy is made.Pan Bi is respectively provided with six different types in six circular holes circumferentially with uniform intervals setting there are six circular hole
Optical filter, every kind of optical filter filter out or filter particular community light, thus as described above, bio-energy according to the present invention turn
Luminescence analyzer is moved, may be implemented to analyze six kinds of different wave lengths to the sample solution in each enzyme mark cup, that is to say, that every kind of filter
Mating plate is corresponding to support sample solution to analyze a kind of wavelength.Filter disc 41 can rotate, to make the optical filtering of specific required type
Piece is located at vertical direction bottom, and the optical filter of type needed for this is specific at this time can most effectively be covered from detection position
The stray light that adjacent optical filter enters.Filter disc receiver assembly 4 further includes motor 43.Filter disc 41 is fixed on 43 motors
On, it is driven by motor 43, the two guarantees concentricity.It is connected, is passed through with pom plastic inner lining 411 between optical filter and filter disc 41
The control of fit tolerance is so that not having to glue can also fix in the space of 3mm, so as to easily replace optical filter.
Filter disc receiver assembly 4 further includes the receiver holder 44 positioned at 42 lower section of receiver.Receiver holder 44 is
Rectangular flat plate, and it is provided with through-hole 441.Receiver 42 includes sensor and is fixed on receiver holder 44, is received
Device 42 and receiver holder 44 are respectively positioned on the inside of filter disc 41, more specifically, be located at filter disc 41 along the vertical direction most
On the inside of the disk wall of lower part, but have no effect on the rotation of filter disc 41.
Filter disc receiver assembly 4 further includes fixed block 45, fixed block 45 be located at filter disc 41 along the vertical direction most
On the outside of the disk wall of lower part, but have no effect on the rotation of filter disc 41.Receiver holder 44 is also secured on fixed block 45.
I other words both receiver holder 44 and fixed block 45 are fixed together and are located at along the vertical direction most lower of filter disc 41
Inside and outside partial Pan Bi, moreover, the gap between receiver holder 44 and fixed block 45 can allow filter disc 41 into
Row rotation.Fixed block 45 is formed as concave surface towards the surface of filter disc 41, so as to the form fit with filter disc 41.It is fixed
Through-hole 451 is also formed on block 45.
Filter disc receiver assembly 4 further includes movable ram 46.Movable ram 46 is similar to bottle cap shape.It specifically, can dynamic pressure
First 46 include cylindrical body, in the top of cylindrical body horizontal-extending edge and at the bottom of cylindrical body outward
The tapered portion formed is held, reference can be made to Figure 17-18.Movable ram 46 further includes through cylindrical body, edge and tapered portion
Through-hole 461.Movable ram 46 is assemblied in the through-hole 451 of fixed block 45, and can be moved up and down in through-hole 451.Work as enzyme
Mark cup assembly transverse movement and make the rim of a cup of an enzyme mark cup move to start contact movable ram 46 when, movable ram 46 will
It is jacked up with the movement of rim of a cup by rim of a cup.When the rim of a cup of this enzyme mark cup moves to the underface of movable ram 46, movably
Pressure head 46 will be moved downward because of gravity is pressed into this enzyme mark cup, such as can be pressed into 0.3mm, so that masking comes from
The light of its rim of a cup of its enzyme mark cup, and only acquire the light of the rim of a cup of this enzyme mark cup immediately below movable ram 46
Line.Hereafter, the sample solution in next enzyme mark cup is analyzed if necessary, and movable ram 46 will be with next enzyme mark cup
Rim of a cup movement and by the rim of a cup of next enzyme mark cup jack up, when the rim of a cup of next enzyme mark cup moves to movable ram
When 46 underface, movable ram 46 will move downward the rim of a cup for being pressed into next enzyme mark cup because of gravity.Afterwards
The movement of other enzyme mark cups of face is similar.
Although in the present invention, showing eight enzyme mark cups and six optical filters, those of ordinary skill in the art in figure
It is understood that the number of enzyme mark cup and optical filter can according to need and change.
In operation, staff opens bio-energy and shifts luminescence analyzer, and the will be put into equipped with sample solution enzyme mark cup
It is provided in recess portion 1211 on the upper side of the right side of the horizontal extension of two housing sections 12.Then it is selected on display operation screen
Required analysis type is selected, driving transmission assembly just makes enzyme mark cup assembly move to filter disc receiver assembly 4, thus to each
Sample solution in enzyme mark cup is analyzed.
In the present specification, whenever referring to " illustrative embodiments ", " preferred embodiment ", " embodiment " etc.
When mean for the embodiment description specific feature, structure or feature include at least one embodiment party of the invention
In formula.The appearance of these words different places in the present specification is not necessarily all referring to same embodiment.In addition, appointing when being directed to
When one embodiment/embodiment describes specific feature, structure or feature, it will be understood that those skilled in the art also can be
This feature, structure or feature are realized in other embodiment in all embodiments.
Embodiments of the present invention are described in detail above.However, aspect of the invention is not limited to above embodiment.In
Without departing from the scope of the invention, various remodeling and replacement can be applied in above embodiment.
Claims (9)
1. a kind of bio-energy shifts luminescence analyzer, which is characterized in that the bio-energy shifts luminescence analyzer and includes:
Outer housing；And
Enzyme mark cup assembly, driving transmission assembly and the filter disc receiver assembly being contained in the outer housing,
Wherein, the enzyme mark cup assembly is driven and is transmitted to the filter disc from initial position and receives by the driving transmission assembly
At device assembly,
The filter disc receiver assembly includes filter disc, receiver and the movable ram being arranged in below the filter disc, and
And wherein,
The filter disc can rotate, for the cylindrical shape bottom of with and with cylindric Pan Bi and circular discs bottom, the Pan Bi
It is provided with circular hole, optical filter is housed in the circular hole,
The movable ram include cylindrical body, the top of the cylindrical body outward horizontal-extending edge and
In the tapered portion that the bottom end of the cylindrical body is formed, and the movable ram can move up and down, when the enzyme mark cup
It contacts and when further towards the movable ram, the tapered portion is jacked up by the rim of a cup of the enzyme mark cup, when the enzyme mark cup
Rim of a cup when moving to the underface of the movable ram, the movable ram moves downward to be pressed into institute by gravity
State enzyme mark cup.
2. bio-energy according to claim 1 shifts luminescence analyzer, which is characterized in that the optical filtering disk receiving group
Part further includes fixed block and receiver holder, and the receiver and the receiver holder are respectively positioned on the inside of the filter disc,
The fixed block is located on the outside of the disk wall of the lowermost part along the vertical direction of the filter disc and including through-hole, the receiver
Bracket is fixed on the fixed block, and the movable ram moves up and down in the through hole.
3. bio-energy according to claim 2 shifts luminescence analyzer, which is characterized in that the Pan Bi is circumferentially with equal
It is even to be arranged at intervals with multiple circular holes, different types of optical filter is respectively provided in the multiple circular hole.
4. bio-energy according to any one of claim 1-3 shifts luminescence analyzer, which is characterized in that the enzyme mark
Cup assembly includes enzyme mark cup base, enzyme mark cup and opening-closing device, and the enzyme mark cup is arranged on the enzyme mark cup base, described
Opening-closing device includes the first opening and closing part and the second opening and closing part, and the first end of the first opening and closing part and the second opening and closing part is with pivotable
Mode is connected to the enzyme mark cup base so that the first opening and closing part and the second opening and closing part can open and drawing, described
The mutually opposed surface of first opening and closing part and the second opening and closing part is provided with the concave surface with the form fit of the enzyme mark cup
Portion.
5. bio-energy according to claim 4 shifts luminescence analyzer, which is characterized in that the opening-closing device further includes
Elastic component, the elastic component be arranged on the first opening and closing part and the second opening and closing part so as to will the first opening and closing part with
The second opening and closing part drawing.
6. bio-energy according to claim 5 shifts luminescence analyzer, which is characterized in that the bio-energy transfer hair
Light analyzer further includes wedge piece, and the wedge piece is fixed on the outer housing, the first opening and closing part and described
The pin extended straight down is respectively arranged at lower surface near the second end of two opening and closing parts, the pin is along the wedge piece
Two sides sliding.
7. bio-energy according to any one of claim 1-3 shifts luminescence analyzer, which is characterized in that the shell
Body includes first shell portion and second shell portion, and the edge joint position between the first shell portion and the second shell portion is provided with
One or more sets joinery and its constructions.
8. bio-energy according to claim 7 shifts luminescence analyzer, which is characterized in that the first shell portion and institute
State second shell portion and be hollow, the second shell portion includes horizontal extension and vertical extension, the first shell
Portion is arranged on the top surface of the left side of the horizontal extension in the second shell portion, the downside in the first shell portion
The opening for penetrating through the first shell portion and the second shell portion is equipped with the upper side in the second shell portion.
9. bio-energy according to claim 8 shifts luminescence analyzer, which is characterized in that the institute in the second shell portion
It states and is provided with recess portion on the upper side of the right side of horizontal extension, the bottom of the recess portion is equipped with opening, the second shell
It is additionally provided with flip lid corresponding to the recess portion in portion, the lower surface of the flip lid is formed with downward projection of protrusion, the flip lid
It is pivotally installed to using shaft in the second shell portion, so that the flip lid is in the closing position for closing the recess portion
It sets and makes to move between the open open position of the recess portion, the periphery of the recess portion is provided with joinery and its construction, the protrusion
Around also correspond to the recess portion and be provided with joinery and its construction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710695840.8A CN107505310B (en) | 2017-08-15 | 2017-08-15 | Bio-energy shifts luminescence analyzer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710695840.8A CN107505310B (en) | 2017-08-15 | 2017-08-15 | Bio-energy shifts luminescence analyzer |
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| Publication Number | Publication Date |
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| CN107505310A CN107505310A (en) | 2017-12-22 |
| CN107505310B true CN107505310B (en) | 2019-11-15 |
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| CN101993956A (en) * | 2010-01-03 | 2011-03-30 | 中国科学院上海微系统与信息技术研究所 | Method for detecting HBV DNA and single base mutation by resonance energy transfer based on quantum dots |
| CN202159063U (en) * | 2011-07-20 | 2012-03-07 | 深圳市汇松科技发展有限公司 | Enzyme-labeling measuring instrument detecting box body |
| CN202562843U (en) * | 2012-04-28 | 2012-11-28 | 嘉兴凯实生物科技有限公司 | Microplate reader suitable for open environment |
| WO2015053290A1 (en) * | 2013-10-07 | 2015-04-16 | ユニバーサル・バイオ・リサーチ株式会社 | Chemiluminescence measurement device and method for same |
| CN204462150U (en) * | 2015-04-07 | 2015-07-08 | 河南工业职业技术学院 | A kind of microplate reader |
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2017
- 2017-08-15 CN CN201710695840.8A patent/CN107505310B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101993956A (en) * | 2010-01-03 | 2011-03-30 | 中国科学院上海微系统与信息技术研究所 | Method for detecting HBV DNA and single base mutation by resonance energy transfer based on quantum dots |
| CN202159063U (en) * | 2011-07-20 | 2012-03-07 | 深圳市汇松科技发展有限公司 | Enzyme-labeling measuring instrument detecting box body |
| CN202562843U (en) * | 2012-04-28 | 2012-11-28 | 嘉兴凯实生物科技有限公司 | Microplate reader suitable for open environment |
| WO2015053290A1 (en) * | 2013-10-07 | 2015-04-16 | ユニバーサル・バイオ・リサーチ株式会社 | Chemiluminescence measurement device and method for same |
| CN204462150U (en) * | 2015-04-07 | 2015-07-08 | 河南工业职业技术学院 | A kind of microplate reader |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107505310A (en) | 2017-12-22 |
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