CN107502557A - A kind of spot jade gill fungus culture medium - Google Patents
A kind of spot jade gill fungus culture medium Download PDFInfo
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- CN107502557A CN107502557A CN201710863705.XA CN201710863705A CN107502557A CN 107502557 A CN107502557 A CN 107502557A CN 201710863705 A CN201710863705 A CN 201710863705A CN 107502557 A CN107502557 A CN 107502557A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The present invention relates to a kind of spot jade gill fungus culture medium, including solid medium and fluid nutrient medium, the composition and content of solid medium are：Potato 100g 200g, agar 10g 20g, glucose 5g 10g, trehalose 5g 10g add distilled water to be settled to 0.5L 1L, and the composition and content of fluid nutrient medium are：Potato 100g 200g, glucose 5g 10g, trehalose 5g 10g, distilled water is added to be settled to 0.5L 1L.The culture medium of the present invention can grow for spot jade gill fungus provides good nutriment and space environment, preparation method is simple, can accelerate the speed of growth of spot jade gill fungus mycelia, significantly improve its hypha biomass, increase economic benefit, the spot jade gill fungus mycelium growth vigor for cultivating to obtain is good.
Description
Technical field
The invention belongs to spot jade gill fungus to cultivate field, more particularly to a kind of spot jade gill fungus culture medium.
Background technology
Spot jade gill fungus (Hypsizigus marmoreus) also known as true pleurotus cornucopiae, crab flavour mushroom, are under the jurisdiction of Basidiomycota
(Basidiomycota), Hymenomycetes (Hymenomycetes), Agaricales (Agaricales), Bai Mo sections
(Tricholomataceae), beautiful gill fungus category (Hypsizigus).Spot jade gill fungus fine and tender taste, delicious flavour, have " fragrant in matsutake, taste
In beautiful gill fungus " good reputation.The mushroom has anti-oxidant, disease-resistant rich in various active compositions such as protein, polysaccharide, amino acid, vitamins
Malicious, hypoglycemic, reducing blood lipid and other effects, have edible medicinal value concurrently.Spot jade gill fungus is in Chinese Shanghai, Jiangsu, Hebei, Shandong, Fujian, wide
East etc. saves (city) by extensive factory culture, and it is before realizing extensive fruiting production to cultivate healthy and strong spot jade gill fungus mycelium
Carry.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of spot jade gill fungus culture medium, and the culture medium preparation method is simple,
It is easy to spread, the speed of growth of spot jade gill fungus mycelia can be accelerated, significantly improve its hypha biomass.
A kind of spot jade gill fungus culture medium of the present invention, including solid medium and fluid nutrient medium, the composition of solid medium
And content is：Potato 100g-200g, agar 10g-20g, glucose 5g-10g, trehalose 5g-10g, distilled water is added to be settled to
0.5L-1L, the composition and content of fluid nutrient medium are：Potato 100g-200g, glucose 5g-10g, trehalose 5g-10g, add
Distilled water is settled to 0.5L-1L.
The solid medium, fluid nutrient medium sterilize 20min at 121 DEG C, to be seeded after cooling.
The inoculation is what is carried out on ultraviolet sterilization 30min superclean bench.
The inoculum concentration of the fluid nutrient medium is 10%.
The solid medium, fluid nutrient medium are cultivated under 25 DEG C of dark conditions, and fluid nutrient medium exists
Cultivated in 25 DEG C of constant-temperature tables of 150rpm rotating speeds, solid medium is cultivated in 25 DEG C of constant incubators.
Fluid nutrient medium 250mL triangular flasks, per bottled 100mL.
Beneficial effect
The culture medium of the present invention can grow for spot jade gill fungus provides good nutritive value and space environment, preparation method letter
It is single, the speed of growth of spot jade gill fungus mycelia can be accelerated, significantly improve its hypha biomass, increase economic benefit, cultivate what is obtained
Spot jade gill fungus mycelium growth vigor is good.
Brief description of the drawings
Fig. 1 is the spot jade gill fungus mycelia (embodiment) of embodiment 1 and the spot jade gill fungus mycelia of comparative example 1 in spot jade gill fungus incubation
(control group) growing state figure；
Fig. 2 is the spot jade gill fungus mycelia (embodiment) of embodiment 1 and the spot jade gill fungus of comparative example 1 during spot jade gill fungus solid culture
Mycelia (control group) speed of growth figure；
Fig. 3 is the spot jade gill fungus mycelia (embodiment) of embodiment 1 and the spot jade gill fungus of comparative example 1 during spot jade gill fungus Liquid Culture
Mycelia (control group) dry weight figure；
Fig. 4 is spot jade gill fungus in spot jade gill fungus mycelia (embodiment) and comparative example 1 in the embodiment 1 that spot jade gill fungus Liquid Culture obtains
The protein electrophorese figure of mycelia (control group).
Embodiment
With reference to specific embodiment, the present invention is expanded on further.It should be understood that these embodiments are merely to illustrate the present invention
Rather than limitation the scope of the present invention.In addition, it is to be understood that after the content of the invention lectured has been read, people in the art
Member can make various changes or modifications to the present invention, and these equivalent form of values equally fall within the application appended claims and limited
Scope.
Embodiment 1
A kind of spot jade gill fungus culture medium includes solid medium and fluid nutrient medium, and the composition and content of solid medium are：
Potato 200g, agar 15g, glucose 10g, trehalose 10g, add distilled water to be settled to 1L, the composition of fluid nutrient medium and contain
Measure and be：Potato 200g, glucose 10g, trehalose 10g, distilled water is added to be settled to 1L, using 250mL triangular flasks, per bottled
100mL fluid nutrient mediums.All culture mediums sterilize 20min at 121 DEG C, to be seeded after cooling.After ultraviolet sterilization 30min
Superclean bench on carry out spot jade gill fungus mycelium inoculation, then cultivated under 25 DEG C of dark conditions, fluid nutrient medium is in 150rpm
Cultivated in 25 DEG C of constant-temperature tables of rotating speed, solid medium is cultivated in 25 DEG C of constant incubators.Spot jade gill fungus incubation is entered
Row monitoring in real time, observes its growing state and colonial morphology, is taken pictures when mycelial growth is to 7d, as a result as implemented in Fig. 1
Shown in example.During solid culture, spot jade gill fungus mycelial growth rate is evaluated, 4d, 8d are handed over cross after inoculation
Fork method measures colony radius, and colony radius difference (mm) and the ratio of growth number of days (d) are mycelial growth rate (mm/d), weight
Again three times, measurement average result is as shown in embodiment in table 1.The inoculum concentration of fluid nutrient medium is 10%, in Liquid Culture process
In, when 10d is cultivated under dark condition, spot jade gill fungus mycelium pellet covers with triangular flask substantially, in the growth vigorous stage.With drying
Filter paper to constant weight (M1) filters mycelium pellet, and mycelium pellet and filter paper are together dried into constant weight (M2), calculate spot jade gill fungus mycelium pellet
Dry weight M=(M2-M1), in triplicate, the mycelium pellet dry weight average result of measurement is as shown in embodiment in table 2.Collect liquid training
The spot jade gill fungus mycelia of base is supported, its protein is extracted using TCA- acetone methods, carries out SDS-PAGE electrophoretic analysis.
Comparative example 1
A kind of spot jade gill fungus often includes solid medium and fluid nutrient medium, the composition and content of solid medium with culture medium
For：Potato 200g, agar 15g, glucose 20g add distilled water to be settled to 1L, and the composition and content of fluid nutrient medium are：Horse
Bell potato 200g, glucose 20g, adds distilled water to be settled to 1L, adds distilled water to be settled to 1L, using 250mL triangular flasks, per bottled
100mL fluid nutrient mediums.All culture mediums sterilize 20min at 121 DEG C, to be seeded after cooling.After ultraviolet sterilization 30min
Superclean bench on carry out the spot jade gill fungus mycelium inoculations of growth conditions same as Example 1, then under 25 DEG C of dark conditions
Culture, fluid nutrient medium are cultivated in 25 DEG C of constant-temperature tables of 150rpm rotating speeds, and solid medium is in 25 DEG C of constant incubators
Culture.Spot jade gill fungus incubation is monitored in real time, its growing state and colonial morphology is observed, enters when mycelial growth is to 7d
Row is taken pictures, as a result as shown in control group in Fig. 1.During solid culture, spot jade gill fungus mycelial growth rate is evaluated,
4d, 8d measure colony radius with crossing method after inoculation, and colony radius difference (mm) and the ratio of growth number of days (d) are
Mycelial growth rate (mm/d), in triplicate, measurement average result is as shown in control group in table 1.The inoculum concentration of fluid nutrient medium
For 10%, during Liquid Culture, when 10d is cultivated under dark condition, spot jade gill fungus mycelium pellet covers with triangular flask substantially, place
In the growth vigorous stage.Mycelium pellet is filtered with the filter paper for being dried to constant weight (M1), mycelium pellet and filter paper are together dried to constant weight
(M2) spot jade gill fungus mycelium pellet dry weight M=(M2-M1), is calculated, in triplicate, in the mycelium pellet dry weight average result such as table 2 of measurement
Shown in control group.The spot jade gill fungus mycelia of fluid nutrient medium is collected, its protein is extracted using TCA- acetone methods, carries out SDS-PAGE
Electrophoretic analysis.
Fig. 1 shows：Compared with the spot jade gill fungus mycelia (control group) of this comparative example 1, the spot jade gill fungus mycelia of embodiment 1 (is implemented
Example) it is pure white sturdy, bacterium colony is dense, neat in edge, and growing way is more preferable.
Fig. 2 shows：Compared with the spot jade gill fungus mycelia (control group) of this comparative example 1, the spot jade gill fungus mycelia of embodiment 1 (is implemented
Example) speed of growth is substantially fast, it illustrate that growth of the culture medium to spot jade gill fungus mycelia in embodiment 1 has facilitation.
Fig. 3 shows：The spot jade gill fungus mycelia (embodiment) of embodiment 1 is dry with the spot jade gill fungus mycelia (control group) of this comparative example 1
Significant difference again be present, the spot jade gill fungus dry mycelial weight of embodiment illustrates the culture medium energy in embodiment 1 apparently higher than control group
Enough dramatically increase the biomass of spot jade gill fungus mycelia.
Fig. 4 shows：The spot jade gill fungus mycelian protein matter band composition (embodiment) of embodiment 1 and the spot jade gill fungus of this comparative example 1
Mycelian protein matter band forms (control group) indistinction, illustrates that the culture medium in embodiment 1 will not change the egg of spot jade gill fungus mycelia
White expression.
Table 1
Table 2
Claims (6)
1. a kind of spot jade gill fungus culture medium, it is characterised in that including solid medium and fluid nutrient medium, the composition of solid medium
And content is：Potato 100g-200g, agar 10g-20g, glucose 5g-10g, trehalose 5g-10g, distilled water is added to be settled to
0.5L-1L, the composition and content of fluid nutrient medium are：Potato 100g-200g, glucose 5g-10g, trehalose 5g-10g, add
Distilled water is settled to 0.5L-1L.
2. according to a kind of spot jade gill fungus culture medium described in claim 1, it is characterised in that the solid medium, Liquid Culture
Base sterilizes 20min at 121 DEG C, to be seeded after cooling.
3. according to a kind of spot jade gill fungus culture medium described in claim 2, it is characterised in that the inoculation is in ultraviolet sterilization
Carried out on 30min superclean bench.
4. according to a kind of spot jade gill fungus culture medium described in claim 1, it is characterised in that the inoculum concentration of the fluid nutrient medium is
10%.
5. according to a kind of spot jade gill fungus culture medium described in claim 1, it is characterised in that the solid medium, Liquid Culture
Base is cultivated under 25 DEG C of dark conditions, and fluid nutrient medium is cultivated in 25 DEG C of constant-temperature tables of 150rpm rotating speeds, solid
Culture medium is cultivated in 25 DEG C of constant incubators.
6. according to a kind of spot jade gill fungus culture medium described in claim 1, it is characterised in that fluid nutrient medium 250mL tri-
Angle bottle, per bottled 100mL.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710863705.XA CN107502557B (en) | 2017-09-22 | 2017-09-22 | Hypsizygus marmoreus culture medium |
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| CN201710863705.XA CN107502557B (en) | 2017-09-22 | 2017-09-22 | Hypsizygus marmoreus culture medium |
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| CN107502557B CN107502557B (en) | 2021-07-09 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101642054A (en) * | 2009-09-04 | 2010-02-10 | 福建农林大学 | Hypsizigus marmoreus and method for establishing laccase transfer system in breeding thereof |
| CN103449914A (en) * | 2013-08-27 | 2013-12-18 | 广西壮族自治区农业科学院植物保护研究所 | Application of trehalose to prolonging storage life of pleurotus ostrcatus strain, and culture mediums and method for prolonging storage life of pleurotus ostrcatus strain |
| CN106119131A (en) * | 2016-06-28 | 2016-11-16 | 河南省农业科学院植物营养与资源环境研究所 | A kind of method alleviating excess copper suppression Growth of Pleurotus Mycelium |
| CN106520579A (en) * | 2016-12-28 | 2017-03-22 | 福建农林大学 | Serratia marcescens fermentation liquor culture medium promoting growth and development of hypsizygus marmoreus |
-
2017
- 2017-09-22 CN CN201710863705.XA patent/CN107502557B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101642054A (en) * | 2009-09-04 | 2010-02-10 | 福建农林大学 | Hypsizigus marmoreus and method for establishing laccase transfer system in breeding thereof |
| CN103449914A (en) * | 2013-08-27 | 2013-12-18 | 广西壮族自治区农业科学院植物保护研究所 | Application of trehalose to prolonging storage life of pleurotus ostrcatus strain, and culture mediums and method for prolonging storage life of pleurotus ostrcatus strain |
| CN106119131A (en) * | 2016-06-28 | 2016-11-16 | 河南省农业科学院植物营养与资源环境研究所 | A kind of method alleviating excess copper suppression Growth of Pleurotus Mycelium |
| CN106520579A (en) * | 2016-12-28 | 2017-03-22 | 福建农林大学 | Serratia marcescens fermentation liquor culture medium promoting growth and development of hypsizygus marmoreus |
Non-Patent Citations (2)
| Title |
|---|
| GANCEDO ET AL: "The importance of a functional trehalose biosynthetic pathway for the life of yeasts and fungi", 《FEMS YEAST RESEARCH》 * |
| 李亚鹏等: "真菌中的海藻糖及其在低温逆境下的作用", 《食用菌学报》 * |
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