CN107501458B - A kind of ratio fluorescent Nano capsule thermometer and preparation method thereof - Google Patents
A kind of ratio fluorescent Nano capsule thermometer and preparation method thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F212/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an aromatic carbocyclic ring
- C08F212/34—Monomers containing two or more unsaturated aliphatic radicals
- C08F212/36—Divinylbenzene
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- C—CHEMISTRY; METALLURGY
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- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F212/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an aromatic carbocyclic ring
- C08F212/02—Monomers containing only one unsaturated aliphatic radical
- C08F212/04—Monomers containing only one unsaturated aliphatic radical containing one ring
- C08F212/06—Hydrocarbons
- C08F212/08—Styrene
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- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F230/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal
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- G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
- G01K11/00—Measuring temperature based upon physical or chemical changes not covered by groups G01K3/00, G01K5/00, G01K7/00 or G01K9/00
- G01K11/12—Measuring temperature based upon physical or chemical changes not covered by groups G01K3/00, G01K5/00, G01K7/00 or G01K9/00 using changes in colour, translucency or reflectance
- G01K11/16—Measuring temperature based upon physical or chemical changes not covered by groups G01K3/00, G01K5/00, G01K7/00 or G01K9/00 using changes in colour, translucency or reflectance of organic materials
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Abstract
The present invention discloses a kind of ratio fluorescent Nano capsule thermometer, and the thermometer is using fluorescent molecule solution as kernel, using high-molecular copolymer as the Nano capsule of shell；Wherein, the fluorescent molecule is N, bis- (the 2- ethylhexyls) -3 of N'-, 4,9,10- 4 formyl-2-imides.The invention also discloses the preparation methods of above-mentioned ratio fluorescent Nano capsule thermometer.The fluorescent molecule N that Nano capsule thermometer of the present invention uses, bis- (the 2- ethylhexyls) -3 of N'-, 4,9,10- 4 formyl-2-imide has many advantages, such as that light, thermal stability are good, quantum yield is high, styrene, divinylbenzene, 3- (isobutene acyl-oxygen) propyl trimethoxy silicane, n-isopropyl acrylamide copolymer have many advantages, such as that bio-compatibility is good, wrap up tight, have a good application prospect.
Description
Technical field
The present invention relates to fluorescence nano field of thermometers, in particular to a kind of ratio fluorescent Nano capsule thermometer and its system
Preparation Method.
Background technique
Temperature suffers from extremely in the physics, chemistry, bioprocess of the overwhelming majority as a very important parameter
Important role.Therefore, the temperature for accurately and quickly measuring system is of great significance.Common thermometer (such as thermal coupling temperature
Degree meter) it is low due to spatial resolution the problems such as, so that it is not able to satisfy the measurement of microbody system (such as individual cells) interior temperature.With
The continuous development of nanotechnology, nanothermometer have been widely studied.Wherein, fluorescence chemical thermometer because have high sensitivity,
Response is got the attention rapidly, convenient for superiority such as cell imagings.In numerous fluorescence chemical thermometers, ratio fluorescent
Thermometer passes through the ratio test temperature of two fluorescence signal values, eliminates accidentalia bring in test process and interferes, tool
Standby better Stability and veracity, therefore it is particularly suited for the detection of intracellular slight temperature changing value.
Nanothermometer (such as CdTe quantum, lanthanide series metal adulterate particle) for cell tests can be because there is biological poison
Property and the exception for causing cell function, at the same time, physicochemical environment in cell also can accuracys and spirit to nanothermometer
Quick property adversely affects, or even it is made to lose temperature sensitive performance.
Therefore, a kind of ratio fluorescent Nano capsule thermometer is developed, the temperature sensitive kernel of thermometer is wrapped in macromolecule
It is copolymerized in shell, it is isolated with cellular component, has a very important significance.
Summary of the invention
It is an object of the present invention to provide a kind of ratio fluorescent Nano capsule thermometers, can be used for small system temperature
Detection.
It is another object of the present invention to provide a kind of preparation methods of above-mentioned fluorescence nano thermometer.
In order to achieve the above objectives, the present invention adopts the following technical solutions:
The present invention provides a kind of ratio fluorescent Nano capsule thermometer, it is interior that the thermometer, which is with fluorescent molecule solution,
Core, using high-molecular copolymer as the Nano capsule of shell；
Wherein, the fluorescent molecule is N, bis- (the 2- ethylhexyls) -3 of N'-, 4,9,10- 4 formyl-2-imides.
Further, the molar concentration of the fluorescent molecule solution is 7.0 × 10-4-3.5×10-3mol/L；
Further, the fluorescent molecule solution is made of fluorescent molecule and organic solvent, and the organic solvent is isooctane
With the mixture of hexadecane；Wherein, the mass ratio of the isooctane and hexadecane is 10-15:1.
Further, the high-molecular copolymer is styrene, divinylbenzene, 3- (isobutene acyl-oxygen) propyl trimethoxy silicon
Alkane and n-isopropyl acrylamide；Wherein, the styrene, divinylbenzene, 3- (isobutene acyl-oxygen) propyl trimethoxy silicane
Mass ratio with n-isopropyl acrylamide is 5-50:1-25:5-25:1:5.
Further, the Nano capsule is sphere, and capsule diameter 50-150nm, capsule shell wall thickness is 10-30nm.
Invention further provides the preparation methods of the ratio fluorescent Nano capsule thermometer, comprising the following steps:
1) fluorescent molecule is dissolved in organic solvent, obtains fluorescent molecule solution, add high-molecular copolymer, mixed, made
For oily phase；
2) PB buffer is added in surfactant solution, mixes, as water phase；
3) oil is mutually poured into water phase, fine crankshaft obtains miniemulsion；
4) N is passed through into miniemulsion2To remove O2, water soluble starter is added, heating is that fluorescent molecule is molten to get kernel
Liquid, shell are the Nano capsule thermometer of high-molecular copolymer.
Further, the mass ratio of the fluorescent molecule solution and high-molecular copolymer is 1.1-1.9:1.
Further, it is 5.65 × 10 that the surfactant, which is concentration,-4-1.67×10-3The dodecyl sodium sulfonate of g/ml
Sodium.
Further, the volume ratio of the PB buffer and aqueous surfactant solution is 1.3 × 10-3-1.7×10-2:1。
Further, the concentration of the PB buffer is 1-10mM, pH 7.4.
Further, the mass ratio of the water phase and oily phase is 8.7-24.1:1.
Further, the water soluble starter is the persulfate aqueous solution that concentration is 0.02-0.04g/ml.
Further, the volume ratio of the water soluble starter and water phase is 0.027-0.075:1.
Further, gained Nano capsule thermometer is distributed in ultrapure water, Thermo-sensitive survey is carried out in Fluorescence Spectrometer
Examination, excitation wavelength 400nm, launch wavelength range are 500-750nm.
In the present invention, bis- (the 2- ethylhexyls) -3 of N, N'-, 4,9,10- 4 formyl-2-imides are pi-conjugated point of plane
Son, easily acts on forming excimer in certain density solution system by π-π, showed in fluorescence spectrum monomer and
The corresponding emission peak of excimer.It will affect intermolecular π-π effect due to changing temperature, move equilibrium system
It is dynamic, so that respectively the corresponding ratio variation for emitting peak area is realized to temperature using monomer in Nano capsule and excimer
Detection.
Beneficial effects of the present invention are as follows:
1, bis- (2- the ethylhexyl) -3,4,9,10- 4 formyl-2-imide tools of fluorescent molecule N, N'- used in the present invention
Have the advantages that light, thermal stability are good, quantum yield is high, styrene, divinylbenzene, 3- (isobutene acyl-oxygen) propyl trimethoxy silicon
Alkane, n-isopropyl acrylamide copolymer have many advantages, such as that bio-compatibility is good, wrap up it is tight, therefore, the present invention in receiving
Rice glue capsule thermometer has a good application prospect.
2, the present invention used in fluorescent molecule only can just show stronger Thermo-sensitive in organic solvent, pass through by
The Thermo-sensitive solution is wrapped in styrene, divinylbenzene, 3- (isobutene acyl-oxygen) propyl trimethoxy silicane, N- isopropyl propylene
A kind of kernel is made in the copolymer of amide to be in a liquid state, shell is in solid Nano capsule thermometer, both ensure that capsule core
The Thermo-sensitive of middle fluorescent molecule solution, in turn avoids interfering with each other for capsule internal and external environment.
3, the present invention by fluorescent molecule not soluble in water be wrapped in can in water phase uniformly, the Nano capsule of stable dispersion
In, it is successfully realized the purpose using organic molecule detection aqueous phase system temperature.The thermometer can be used for small aqueous phase system temperature
The detection of degree has prospect in unicellular temperature detection.
Detailed description of the invention
Specific embodiments of the present invention will be described in further detail with reference to the accompanying drawing.
Fig. 1 shows the structural schematic diagram of embodiment 1-4 Medium Fluorescence Ratio Nano capsule thermometer.
Fig. 2 shows the local high powers of transmission electron microscope picture (a) the low power figure (b) of the Nano capsule thermometer of the preparation of embodiment 1 to put
Big figure.
Fig. 3 shows the dynamic light scattering result of the Nano capsule thermometer of the preparation of embodiment 1.
Fig. 4 shows Nano capsule thermometer monomer prepared by embodiment 2 and aggregation corresponds to the area ratio of emission peak
(S500-620/S620-750) variation with temperature, range of temperature is 5 DEG C -65 DEG C, is divided into 10 DEG C.
Fig. 5 shows Nano capsule thermometer monomer prepared by embodiment 3 and aggregation corresponds to the area ratio of emission peak
(S500-620/S620-750) with the variation of temperature cycles, temperature change is 40 DEG C to 30 DEG C, and circulation is three times.
Fig. 6 shows Nano capsule thermometer monomer prepared by embodiment 4 and aggregation corresponds to the area ratio of emission peak
(S500-620/S620-750) with the variation of bovine serum albumin(BSA) concentration in solution, temperature range is 5 DEG C -65 DEG C, is divided into 20 DEG C, ox
Sero-abluminous concentration is respectively 0,1.1,3.2mg/mL.
Specific embodiment
In order to illustrate more clearly of the present invention, the present invention is done further below with reference to preferred embodiments and drawings
It is bright.Similar component is indicated in attached drawing with identical appended drawing reference.It will be appreciated by those skilled in the art that institute is specific below
The content of description is illustrative and be not restrictive, and should not be limited the scope of the invention with this.
A kind of ratio fluorescent Nano capsule thermometer of embodiment 1
A kind of preparation method of ratio fluorescent Nano capsule thermometer, comprising the following steps:
(1) synthesis of fluorescent molecule
20mmol 2- ethyl -1- hexylamine, 5mmol zinc acetate (Zn (OAc) are added in 10mmol tetracarboxylic acid dianhydride2),
100mL N, N '-dimethyl acetamide stirs 3 hours at 90 DEG C under protection of argon gas.After reaction, revolving removes molten
Agent N, N '-dimethyl acetamide, is stirred overnight after adding 400mL HCl (1mol/L), and filtering can obtain dark red precipitate, use water
It is sufficiently washed with methanol；It is finally dried at 100 DEG C to get solid fluorescence molecule N, N'- bis- (2- ethylhexyls) -3,4,9,
10- 4 formyl-2-imide.
(2) preparation of Nano capsule
1) it takes 0.001mmol fluorescent molecule to be dissolved in 1.4g isooctane, after ultrasound mixes, 0.1g hexadecane, system is added
The standby fluorescent molecule solution that obtains is to get the core liquid of Nano capsule；0.1g styrene is added in above-mentioned fluorescent molecule solution,
0.50g divinylbenzene, 0.1g 3- (isobutene acyl-oxygen) propyl trimethoxy silicane, the mixing of 0.1g n-isopropyl acrylamide are equal
It is even to be used as oily phase；
2) 0.033g dodecyl sodium sulfate is weighed, 20g ultrapure water and 0.1g PB buffer (10mM, pH=is added
7.4) it, is uniformly mixed and is used as water phase；
3) water phase is slowly mutually poured by oily, is transferred to two mouthfuls of round-bottomed flasks, 40 DEG C of oil bath heatings, 400 revs/min are stirred 60 points
Zhong Hou carries out fine crankshaft using cell disruptor, obtains miniemulsion, wherein the condition of the fine crankshaft is that power is 80%, temperature
Spending the upper limit is 40 DEG C, and the time is 10 minutes；
4) N is passed through into miniemulsion230 minutes to remove O2, while in N270 DEG C are heated under protection, mixing speed is
400 revs/min；0.05g potassium peroxydisulfate is weighed, is dissolved in 1.5ml water, sonic oscillation is allowed to be completely dissolved, will by syringe
Persulfate aqueous solution is slowly added into flask, and heating generates a large amount of free radicals, promotes capsule wall comonomer in grease circle
Generation is free-radical polymerized at face, stops heating after 5 hours, and natural cooling is fluorescent molecule solution (liquid), shell to get kernel
For the Nano capsule thermometer of high-molecular copolymer (solid-state).
Described Nano capsule thermometer structural schematic diagram such as Fig. 1, transmission electron microscope picture such as Fig. 2, dynamic light scattering result is such as
Fig. 3.It can be seen from the figure that the core-shell structure of Nano capsule is obvious, particle diameter distribution Relatively centralized.
A kind of ratio fluorescent Nano capsule thermometer of embodiment 2
A kind of preparation method of ratio fluorescent Nano capsule thermometer, comprising the following steps:
(1) synthesis of fluorescent molecule
25mmol 2- ethyl -1- hexylamine, 4mmol zinc acetate (Zn (OAc) are added in 10mmol tetracarboxylic acid dianhydride2),
140mL N, N '-dimethyl acetamide stirs 4 hours at 100 DEG C under protection of argon gas.After reaction, revolving removes molten
Agent N, N '-dimethyl acetamide, is stirred overnight after adding 400mL HCl (2mol/L), and filtering can obtain dark red precipitate, use water
It is sufficiently washed with methanol.It is finally dried at 150 DEG C to get solid fluorescence molecule N, N'- bis- (2- ethylhexyls) -3,4,9,
10- 4 formyl-2-imide.
(2) preparation of Nano capsule
1) it takes 0.004mmol fluorescent molecule to be dissolved in 1.4g isooctane, after ultrasound mixes, 0.14g hexadecane, system is added
The standby fluorescent molecule solution that obtains is to get the core liquid of Nano capsule；1.0g styrene, 0.1g bis- are added in fluorescent molecule solution
Ethenylbenzene, 0.25g 3- (isobutene acyl-oxygen) propyl trimethoxy silicane, 0.02g n-isopropyl acrylamide, which is uniformly mixed, to be made
For oily phase.
2) 0.06g dodecyl sodium sulfate is weighed, 36g ultrapure water and 0.2g PB buffer (10mM, pH=7.4) is added,
It is uniformly mixed and is used as water phase.
3) water phase is slowly mutually poured by oily, is transferred to two mouthfuls of round-bottomed flasks, 45 DEG C of oil bath heatings, 430 revs/min are stirred 90 points
Zhong Hou carries out fine crankshaft using cell disruptor, obtains miniemulsion, wherein the condition of the fine crankshaft is that power is 85%, temperature
Spending the upper limit is 45 DEG C, and the time is 8 minutes.
4) N is passed through into miniemulsion235 minutes to remove O2, while in N272 DEG C are heated under protection, mixing speed is
450 revs/min；0.048g potassium peroxydisulfate is weighed, is dissolved in 1.5ml water, sonic oscillation is allowed to be completely dissolved, will by syringe
Persulfate aqueous solution is slowly added into flask, and heating generates a large amount of free radicals, promotes capsule wall comonomer in grease circle
Generation is free-radical polymerized at face, stops heating after 6 hours, and natural cooling is fluorescent molecule solution (liquid), shell to get kernel
For the Nano capsule thermometer of high-molecular copolymer (solid-state).
Gained Nano capsule thermometer is distributed in ultrapure water, Thermo-sensitive test, excitation are carried out in Fluorescence Spectrometer
Wavelength is 400nm, and launch wavelength range is 500-750nm.Range of measuring temp is 5-65 DEG C, is divided into 10 DEG C, test result
Such as Fig. 4, as can be seen from the figure: Nano capsule thermometer monomer and aggregation correspond to the area ratio (S of emission peak500-620/
S620-750) with temperature have good linear response.
A kind of ratio fluorescent Nano capsule thermometer of embodiment 3
A kind of preparation method of ratio fluorescent Nano capsule thermometer, comprising the following steps:
(1) synthesis of fluorescent molecule
30mmol 2- ethyl -1- hexylamine, 4mmol zinc acetate (Zn (OAc) are added in 10mmol tetracarboxylic acid dianhydride2),
160mL N, N '-dimethyl acetamide stirs 5 hours at 110 DEG C under protection of argon gas.After reaction, revolving removes molten
Agent N, N '-dimethyl acetamide, is stirred overnight after adding 400mL HCl (3mol/L), and filtering can obtain dark red precipitate, use water
It is sufficiently washed with methanol.It is finally dried at 120 DEG C to get solid fluorescence molecule N, N'- bis- (2- ethylhexyls) -3,4,9,
10- 4 formyl-2-imide.
(2) preparation of Nano capsule
1) it takes 0.004mmol fluorescent molecule to be dissolved in 1.4g isooctane, after ultrasound mixes, 0.14g hexadecane, system is added
The solution of standby fluorescent molecule to get Nano capsule core liquid；0.5g styrene is added in above-mentioned fluorescent molecule solution,
0.03g divinylbenzene, 0.6g 3- (isobutene acyl-oxygen) propyl trimethoxy silicane, the mixing of 0.04g n-isopropyl acrylamide
Uniformly as oily phase；
2) 0.02g dodecyl sodium sulfate is weighed, 36g ultrapure water and 0.6g PB buffer (10mM, pH=7.4) is added,
It is uniformly mixed and is used as water phase；
3) water phase is slowly mutually poured by oily, is transferred to two mouthfuls of round-bottomed flasks, 43 DEG C of oil bath heatings, 450 revs/min of stirrings 100
After minute, fine crankshaft being carried out using cell disruptor, obtains miniemulsion, wherein the condition of the fine crankshaft is that power is 80%,
Temperature upper limit is 43 DEG C, and the time is 12 minutes；
4) N is passed through into miniemulsion240 minutes to remove O2, while in N274 DEG C are heated under protection, mixing speed is
430 revs/min；0.055g potassium peroxydisulfate is weighed, is dissolved in 1.5ml water, sonic oscillation is allowed to be completely dissolved, will by syringe
Persulfate aqueous solution is slowly added into flask, and heating generates a large amount of free radicals, promotes capsule wall comonomer in grease circle
At face occur it is free-radical polymerized, after 5.5 hours stop heating, natural cooling to get.
Gained Nano capsule thermometer is distributed in ultrapure water, Thermo-sensitive test, excitation are carried out in Fluorescence Spectrometer
Wavelength is 400nm, and launch wavelength range is 500-750nm.As a result as shown in figure 5, as can be seen from the figure: being cooled to from 40 DEG C
30 DEG C, then 40 DEG C are warming up to from 30 DEG C, it recycles three times, the fluorescence peak area rate value variation tendency one that heating and cooling obtain
It causes, illustrates that Nano capsule thermometer has preferable invertibity.
4 ratio fluorescent Nano capsule thermometer of embodiment
A kind of preparation method of ratio fluorescent Nano capsule thermometer, comprising the following steps:
(1) synthesis of fluorescent molecule
30mmol 2- ethyl -1- hexylamine, 4mmol zinc acetate (Zn (OAc) are added in 10mmol tetracarboxylic acid dianhydride2),
2000mL N, N '-dimethyl acetamide stirs 4 hours at 110 DEG C under protection of argon gas.After reaction, revolving removes
Solvent N, N '-dimethyl acetamide, is stirred overnight after adding 400mL HCl (2mol/L), and filtering can obtain dark red precipitate, uses
Water and methanol sufficiently wash.It is finally dried at 120 DEG C to get solid fluorescence molecule N, N'- bis- (2- ethylhexyls) -3,4,9,
10- 4 formyl-2-imide.
(2) preparation of Nano capsule
It takes 0.007mmol fluorescent molecule to be dissolved in 1.4g isooctane, after ultrasound mixes, 0.14g hexadecane, preparation is added
The solution of fluorescent molecule is obtained to get the core liquid of Nano capsule；0.14g styrene, 0.10g are added in fluorescent molecule solution
Divinylbenzene, 0.5g 3- (isobutene acyl-oxygen) propyl trimethoxy silicane, 0.04g n-isopropyl acrylamide, which is uniformly mixed, to be made
For oily phase；
2) 0.046g dodecyl sodium sulfate is weighed, 56g ultrapure water and 0.075g PB buffer (10mM, pH=is added
7.4) it, is uniformly mixed and is used as water phase；
3) water phase is slowly mutually poured by oily, is transferred to two mouthfuls of round-bottomed flasks, 42 DEG C of oil bath heatings, 460 revs/min of stirrings 120
After minute, fine crankshaft being carried out using cell disruptor, obtains miniemulsion, wherein the condition of the fine crankshaft is that power is 80%,
Temperature upper limit is 42 DEG C, and emulsification times are 10 minutes.
4) N is passed through into miniemulsion260 minutes to remove O2, while in N273 DEG C are heated under protection, mixing speed is
500 revs/min.0.058g potassium peroxydisulfate is weighed, is dissolved in 1.5ml water, sonic oscillation is allowed to be completely dissolved, will by syringe
Persulfate aqueous solution is slowly added into flask, and heating generates a large amount of free radicals, promotes capsule wall comonomer in grease circle
At face occur it is free-radical polymerized, after 5.5 hours stop heating, natural cooling to get.
Gained Nano capsule thermometer is distributed in ultrapure water, Thermo-sensitive test, excitation are carried out in Fluorescence Spectrometer
Wavelength is 400nm, and launch wavelength range is 500-750nm.Temperature-measuring range is 5-65 DEG C, and temperature interval is 20 DEG C, Nano capsule
The concentration of bovine serum albumin(BSA) is respectively 0,1.1,3.2mg/mL in thermometer decentralized medium, as a result as shown in fig. 6, capsule outer ring
The concentration of bovine serum albumin(BSA) has not significant impact the temperature sensitive performance of Nano capsule thermometer in border.
Obviously, the above embodiment of the present invention be only to clearly illustrate example of the present invention, and not be pair
The restriction of embodiments of the present invention may be used also on the basis of the above description for those of ordinary skill in the art
To make other variations or changes in different ways, all embodiments can not be exhaustive here, it is all to belong to this hair
The obvious changes or variations that bright technical solution is extended out are still in the scope of protection of the present invention.
Claims (13)
1. a kind of ratio fluorescent Nano capsule thermometer, which is characterized in that the thermometer be using fluorescent molecule solution as kernel,
Using high-molecular copolymer as the Nano capsule of shell；
Wherein, the fluorescent molecule is N, bis- (the 2- ethylhexyls) -3 of N'-, 4,9,10- 4 formyl-2-imides；
The high-molecular copolymer is different by styrene, divinylbenzene, 3- (isobutene acyl-oxygen) propyl trimethoxy silicane and N-
Four kinds of monomer copolymerization of propylacrylamide obtain.
2. ratio fluorescent Nano capsule thermometer according to claim 1, which is characterized in that the fluorescent molecule solution
Molar concentration is 7.0 × 10-4-3.5×10-3mol/L。
3. ratio fluorescent Nano capsule thermometer according to claim 1, which is characterized in that the fluorescent molecule solution by
Fluorescent molecule and organic solvent are made, and the organic solvent is the mixture of isooctane and hexadecane.
4. ratio fluorescent Nano capsule thermometer according to claim 3, which is characterized in that the isooctane and positive 16
The mass ratio of alkane is 10-15:1.
5. ratio fluorescent Nano capsule thermometer according to claim 1, which is characterized in that the styrene, divinyl
The mass ratio of benzene, 3- (isobutene acyl-oxygen) propyl trimethoxy silicane and n-isopropyl acrylamide is 5-50:25-1:5-25:
5-1。
6. ratio fluorescent Nano capsule thermometer according to claim 1, which is characterized in that the Nano capsule is ball
Body, capsule diameter 50-150nm, capsule shell wall thickness are 10-30nm.
7. a kind of preparation method of any ratio fluorescent Nano capsule thermometer of claim 1-6, which is characterized in that including
Following steps:
1) fluorescent molecule is dissolved in organic solvent, obtains fluorescent molecule solution, added and generate used in high-molecular copolymer
Monomer mixes, as oily phase；
2) surfactant is soluble in water, PB buffer is added, mixes, as water phase；
3) oil is mutually poured into water phase, fine crankshaft obtains miniemulsion；
4) N is passed through into miniemulsion2To remove O2, be added water soluble starter after, heating to get.
8. preparation method according to claim 7, which is characterized in that the fluorescent molecule solution and high-molecular copolymer
Mass ratio is 1.1-1.9:1.
9. preparation method according to claim 7, which is characterized in that the surfactant is that concentration is 5.65 × 10-4-
1.67×10-3The dodecyl sodium sulfate of g/ml.
10. preparation method according to claim 9, which is characterized in that the PB buffer and aqueous surfactant solution
Volume ratio be 1.3 × 10-3-1.7×10-2:1。
11. preparation method according to claim 7, which is characterized in that the mass ratio of the water phase and oily phase is 8.7-
24.1:1。
12. preparation method according to claim 7, which is characterized in that the water soluble starter is that concentration is 0.02-
The persulfate aqueous solution of 0.04g/ml.
13. preparation method according to claim 12, which is characterized in that the volume ratio of the water soluble starter and water phase
For 0.027-0.075:1.
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