CN107495963A - Reusable lightweight fibre electrode and preparation method thereof, embedding method - Google Patents
Reusable lightweight fibre electrode and preparation method thereof, embedding method Download PDFInfo
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- CN107495963A CN107495963A CN201710675948.0A CN201710675948A CN107495963A CN 107495963 A CN107495963 A CN 107495963A CN 201710675948 A CN201710675948 A CN 201710675948A CN 107495963 A CN107495963 A CN 107495963A
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- optical fiber
- laser diode
- pcb board
- electrode
- tetrode
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/24—Detecting, measuring or recording bioelectric or biomagnetic signals of the body or parts thereof
- A61B5/25—Bioelectric electrodes therefor
- A61B5/279—Bioelectric electrodes therefor specially adapted for particular uses
- A61B5/291—Bioelectric electrodes therefor specially adapted for particular uses for electroencephalography [EEG]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/68—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient
- A61B5/6846—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive
- A61B5/6867—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive specially adapted to be attached or implanted in a specific body part
- A61B5/6868—Brain
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D1/00—Surgical instruments for veterinary use
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/40—Animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/42—Evaluating a particular growth phase or type of persons or animals for laboratory research
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2562/00—Details of sensors; Constructional details of sensor housings or probes; Accessories for sensors
- A61B2562/02—Details of sensors specially adapted for in-vivo measurements
- A61B2562/0209—Special features of electrodes classified in A61B5/24, A61B5/25, A61B5/283, A61B5/291, A61B5/296, A61B5/053
Abstract
A repeated use of lightweight fibre electrode provided by the invention and preparation method thereof, embedding method, laser diode is connected with optical fiber one end, nerve signal weld is on pcb board, Electricity Federation between nerve signal joint and pcb board, pcb board is welded on micro-thruster, iron plate is vertically fixed on micro-thruster, capillary guiding tube is adhered on iron plate, tetrode penetrates capillary guiding tube, one end of tetrode protrudes from the length of capillary guiding tube, the hole of the other end insertion pcb board of tetrode, glue curing is used between laser diode optical fiber complex and quadrupole pipe electrode, micro-thruster is fixed in one end of connecting rod, the other end of connecting rod is fixed on laser diode base.Fine wire traction control laser diode is only used, without plug optical fiber and ferrule, therefore can be easily in the multiple recording and synchronous multi-modal light stimulus control for freely realizing that two or more brain area is stablized on the mouse of activity.Small volume, light weight, it can be reused, so that use cost is greatly lowered.
Description
Technical field
The present invention relates to fibre electrode technical field, and in particular to reusable lightweight fibre electrode and its making
Method, embedding method.
Background technology
Light science of heredity is current manipulation neuron and neural circuitry activity most popular method.By being coupled with electrod-array
Mode, using multiple recording technology, it is effective under concrete conditions in the establishment of a specific crime can to verify that special wavelength light is controlled neuron activity
Property.Existing fibre electrode is often with there is following deficiency：(1) before carrying out light stimulus, porcelain bushing need to be inserted optical fibers into.Due to pottery
Porcelain bushing shell one end is connected with recording electrode, therefore inevitably causes the displacement of recording electrode tip, so as to reduce neuron activity
The stability of record.(2) volume is larger, therefore is merely capable of recording in mouse and controls single brain area, simply verifies photocontrol
Effect, and activation record and control can not be carried out in two or more brain area simultaneously.(3) it is difficult to carry out multi-modal photocontrol, electricity
Pole tip is typically only capable to export a kind of light of color.Light beam to send different modalities, then must increase number of fibers, certainly will
Influence the free mobility of animal (particularly mouse).(4) due to electrode fragility, fibre electrode in use easily by
Damage, there is very high requirement to the professional and measure of precision of user's operation；(5) it is difficult to be used by recovery again.Optical fiber electricity
Pole it is expensive, it is impossible to use cost can be significantly greatly increased by being reused.
A kind of light-duty reusable microelectrode thruster is disclosed in Chinese patent 201610987063.X, including
Substrate, guide pin, movable block, screw and conduit, guide pin are vertically provided on substrate, and movable block slides along guide pin, screw
Coordinating with movable block screw thread, screw is fixed axially through position limiting structure, is rotated by screw and is driven movable block to be moved along guide pin,
Conduit is located on movable block to be oriented to microelectrode.The microelectrode thruster of the present invention is compact-sized, small volume, weight
Gently, it is reusable, studied especially suitable for Small Rodents animal (mouse), can be in the brain cloth of Small Rodents animal
Put the microelectrode thruster of at least two present invention.Utilization to microelectrode thruster, it can effectively avoid the deficiency of fibre electrode.
The content of the invention
For in the prior art the defects of, the present invention provides a kind of reusable lightweight fibre electrode and its making
Method, embedding method, it is stability height, small volume, reusable.
A kind of reusable lightweight fibre electrode provided by the invention, including laser diode, optical fiber, nerve letter
Number joint, pcb board, micro-thruster, iron plate, capillary guiding tube, tetrode, connecting rod, laser diode are connected with optical fiber one end
Connect, for nerve signal weld on pcb board, Electricity Federation between nerve signal joint and pcb board, pcb board is welded on micro-thruster
On, iron plate is vertically fixed on micro-thruster, and capillary guiding tube is adhered on iron plate, and tetrode penetrates capillary guiding tube, quadrupole
One end of pipe protrudes from the length of capillary guiding tube, and the tetrode other end inserts the hole of pcb board, and laser diode-optical fiber is multiple
Glue curing is used between fit and quadrupole pipe electrode, micro-thruster is fixed in one end of connecting rod, and the other end of connecting rod is fixed
In laser diode base.
A kind of preparation method of reusable lightweight fibre electrode, comprises the following steps：
S1, laser diode is accurately aligned with optical fiber one end, is bonded using optical fiber glue；
S2, by nerve signal weld on pcb board；
S3, pcb board is welded on micro-thruster, then the perpendicular position of iron plate is fixed on the propeller；
S4, using glue capillary guiding tube is adhered on iron plate；
S5, tetrode is penetrated to capillary guiding tube, one end of tetrode protrudes from the length of capillary guiding tube；
S6, the hole by the other end insertion pcb board of tetrode；
S7, using connecting rod connect laser diode-optical fiber complex, one end of connecting rod is fixedly welded on micromass culture
Device, the other end are fixed on laser diode base.
Further, in S1, the concrete mode being bonded using optical fiber glue is that optical fiber glue is added into laser diode
Between optical fiber, using ultraviolet light curing optical fiber glue, be repeated several times, until optical fiber glue it is fully wrapped around live laser diode and
Space between optical fiber.
Further, in S2, nerve signal weld is subjected to each welding point using epoxy resin afterwards on pcb board
Between insulation and connection solidification.
Further, in S5, tetrode is 4, arranged in parallel between tetrode.
Further, the specific method of the hole of the other end insertion pcb board of tetrode is：By the wire electrode of tetrode
The other end inserts the hole of pcb board, then with metal pin insertion apertures hole, is stripped off the insulating barrier of wire electrode, so as to establish pcb board
Electricity between epineural active joint and wire electrode contacts.
Further, in S7：Under the microscope by laser diode-optical fiber complex be parallel to quadrupole pipe electrode it
Before, gradually against the two, afterwards, optical fiber glue, the solidified glue under ultraviolet light, by the pole of laser two are added between the two
Pipe-optical fiber complex is coupled with quadrupole pipe electrode, and then by connecting rod by laser diode-optical fiber complex with it is micro-
Propeller is connected.
A kind of embedding method of reusable lightweight fibre electrode, comprises the following steps：
A, mouse is pre-processed：Using isoflurane anesthesia mouse, its skull is fixed, keeps skull horizontal, removes small
The calvarium portion hair of mouse, iodophor disinfection skin, then iodine is taken off with 75% alcohol, skin and connective tissue are cut, is exposed at the top of skull,
Exposure range from 1mm before bregma area to cerebellum above, using 10% hydrogen peroxide Sterile surgery area, then gently scrape with tweezers top cranium
Bone, make skull surface coarse；
B, telltale mark：Whether horizontal using registration arm right arm detection skull, the difference of the height of bregma and posterior must be small
In 200um, pilot pin is re-moved to bregma, and the 1.8mm after coordinate is bregma, skull center line right side is other to open 1.3mm's
Do telltale mark in site；
C, the embedding reference electrode in skull area and ground wire electrode screw at the top of cerebellum；
D, ground wire and reference electrode screw exposed portion are covered using cement, forms rectangular dental cement wall；
E, fibre electrode is slowly implanted into brain tissue, is initially implanted into depth 70um, and by the fibre electrode bottom of implantation
It is fixed on dental cement wall.
F, respectively it is adhered fixed equilateral triangle copper mesh on the front, rear, left and right wall of dental cement wall；
G, neuron is provided into signal record weld on the both sides copper mesh holded up；
H, a joint is welded in dental cement wall side copper mesh upper limb inner face, connects laser diode driver line, with to sharp
Optical diode provides dc source.
Further, it is further comprising the steps of：I, the front and rear sides copper mesh on dental cement wall is holded up, and with left and right
Both sides copper mesh is welded to each other fixation, forms the gauze screen for preventing extraneous electric signal interference.
Further, it is further comprising the steps of：J, appropriate dentistry water is smeared into copper mesh bottom surface and musculature adjacent
Mud, reduce influence of the musculature point activity to extracellular recording signal；
K, copper mesh upper end open is sealed using self adhesive bandage, hits or pollute fibre electrode to prevent foreign matter from entering；
L, micro-thruster advances downwardly 70um, examines micro-thruster and optical fiber-electrode coupling body could normal work；
M, mouse is put back into raising box, single cage raising.
As shown from the above technical solution, beneficial effects of the present invention：
The present invention provides a kind of reusable lightweight fibre electrode and preparation method thereof, embedding method, laser two
Pole pipe is connected with optical fiber one end, and nerve signal weld is on pcb board, Electricity Federation between nerve signal joint and pcb board,
Pcb board is welded on micro-thruster, and iron plate is vertically fixed on micro-thruster, and capillary guiding tube is adhered on iron plate, tetrode
Capillary guiding tube is penetrated, one end of tetrode protrudes from the length of capillary guiding tube, the hole of the other end insertion pcb board of tetrode
Hole, laser diode-glue curing is used between optical fiber complex and quadrupole pipe electrode, micromass culture is fixed in one end of connecting rod
Device, the other end of connecting rod are fixed on laser diode base.Fine wire traction control laser diode is only used, without pulling out
Optical fiber and ferrule are inserted, therefore the multichannel that two or more brain area is stablized can be realized on freely movable mouse easily
Record and synchronous multi-modal light stimulus control.Small volume, light weight, it can be reused, so that use cost is greatly lowered.
Brief description of the drawings
, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical scheme of the prior art
The required accompanying drawing used is briefly described in embodiment or description of the prior art.In all of the figs, similar element
Or part is typically identified by similar reference.In accompanying drawing, each element or part might not be drawn according to the ratio of reality.
Fig. 1 is a kind of structural representation of reusable lightweight fibre electrode of the present invention.
Fig. 2 is a kind of embedding schematic top plan view of reusable lightweight fibre electrode of the present invention.
Reference：
1- laser diodes, 2- optical fiber, 3-PCB plates, 4- micro-thrusters, 5- iron plates, 6- capillary guiding tubes, 7- tetrodes,
8- connecting rods, 9- laser diode driver joints, 10- nerve signal joints.
Embodiment
The embodiment of technical solution of the present invention is described in detail below in conjunction with accompanying drawing.Following examples are only used for
Clearly illustrate technical scheme, therefore be only used as example, and the protection model of the present invention can not be limited with this
Enclose.
It should be noted that unless otherwise indicated, technical term or scientific terminology used in this application should be this hair
The ordinary meaning that bright one of ordinary skill in the art are understood.
Refer to Fig. 1 to Fig. 2, a kind of reusable lightweight fibre electrode that the present embodiment provides, including laser
Diode, optical fiber, nerve signal joint, pcb board, micro-thruster, iron plate, capillary guiding tube, tetrode, connecting rod, laser two
Pole pipe is connected with optical fiber one end, and nerve signal weld is on pcb board, Electricity Federation between nerve signal joint and pcb board,
Pcb board is welded on micro-thruster, and iron plate is vertically fixed on micro-thruster, and capillary guiding tube is adhered on iron plate, tetrode
Capillary guiding tube is penetrated, one end of tetrode protrudes from the length of capillary guiding tube, the hole of the other end insertion pcb board of tetrode
Hole, laser diode-glue curing is used between optical fiber complex and tetrode, micro-thruster is fixed in one end of connecting rod, even
The other end of extension bar is fixed on laser diode base.
Laser diode selects Osram companies, model:PL520B or PL450B；Fiber selection Thorlabs companies, type
Number:FT200EMT multimode fibre, length:35mm, diameter:200um；Capillary guiding tube selects Polymicro
Technologies companies, model:TSP100170 silica capillary guiding tube, length:10mm, internal diameter:100um, outside
Footpath:170um；Iron plate selects the wide 2mm x thickness 0.5mm of long 10mm x tagger；Tetrode is from insulation tungsten filament California
Fine Wire companies, model：0.0008 " Tungsten 99.95%, diameter:20um, length:25cm)；Pcb board is from length
The wide 8mm of 12mm x, its surface have printed and Omnetics (models：A79016-001) the corresponding circuit of nerve signal joint and
Connect hole, 1 piece of quantity.Filled gold pin selects Neuralynx companies, model：EIB Pins Small 1000；Optical fiber glue
Wet concentration Thorlabs companies, model:NOA61；Light intensity Yi Xuan Thorlabs companies, model:PM130D；Receptor is selected
Thorlabs companies, model:S130C；Laser diode generating stream controller selects Thorlabs companies, model:LDC-205C；
Impulse generator selects Sanworks companies, model：Pulse Pal v2；Glue selects Loctitte454, and connecting rod is not from
Become rusty steel pole.
The preparation method of reusable lightweight fibre electrode, comprises the following steps：
Under the microscope, laser diode light source is accurately aligned with multimode fibre one end.By another in multimode fibre
The penetrating light intensity detection at end, determines coupling efficiency.When the end light intensity reaches the intensity of light source 15% and the above, laser can be considered as
Diode light-source-fiber coupling success.
Carefully optical fiber glue is added between laser diode and optical fiber, utilizes ultraviolet light curing optical fiber glue.Repeatedly
For several times, until the fully wrapped around firmly space between laser diode and optical fiber of optical fiber glue.And then wrapped up and swashed with dental cement
Optical diode and optical fiber connecting portion, further strengthen standby after fixing.
By Omnetics nerve signal welds on pcb board.After the completion of welding, each welding need to be carried out with epoxy resin
Insulation and connection solidification between joint.
Pcb board with Omnetics nerve signal joints is welded on micro-thruster, reuses dental cement, will be thin
The perpendicular position fixation of iron plate is on this propeller.
Using Loctitte454 glue, 4 silica capillaries are abreast adhered on tagger.Treat that glue coagulates
After Gu, then reinforced with dental cement.The arrangement mode of silica capillary can be adjusted according to experiment needs.
Under the microscope, the tungsten filament quadrupole pipe electrode prepared is penetrated into silica capillary guiding tube.Guiding tube can
Ensure the arranged in parallel of 4 quadrupole pipe electrodes.The position that 4 quadrupole pipe electrodes pass guiding tube lower end uses Loctitte454
Glue is fixed, and prevents quadrupole pipe electrode from sliding.
The other end of tetrode wire electrode needs to penetrate the hole of pcb board, then with metal pin insertion apertures hole, makes wire electrode
Insulating barrier is stripped off, and is contacted so as to establish the electricity between pcb board epineural active joint and wire electrode.Use again
Loctitte454 glue fixes the position that quadrupole pipe electrode passes guiding tube upper end.
Under the microscope, 4 tetrode eletrode tips are had one's hair trimmed, makes its length consistent, protrude from silica guiding tube
Partial length is about 7mm.
Under the microscope, before laser diode and optical fiber complex being parallel into quadrupole pipe electrode, the two is gradual
Abut.Afterwards, optical fiber glue is added between, by glue curing under ultraviolet light.
The stainless steel for being about 5-10mm with a length, its one end are fixedly welded on micro-thruster, and the other end is then fixed on
Laser diode base.This allows laser diode-optical fiber-tetrode electrod-array coupling body with the work of micro-thruster
Move and promote movement.
Finally, wrapped up with black prepared Chinese ink and smear laser diode-optical fiber-tetrode array coupling body, only expose the coupling
The 5mm regions at body tip.The scattering of light in experimentation can so be reduced.
Light source is used as using laser diode.Due to laser diode small volume, therefore can be easily at the top of animal head
2-4 laser diode is installed, for producing the light beam of identical or different mode (such as green glow, feux rouges and blue light).Due to laser
Diode is controlled using mA level constant currents, and by connecting current controller, the 12 of a diameter 0.5mm leads enamel-covered wire
To realize the control to up to 6 laser diodes.Under conditions of it can produce multi-modal light beam, moreover it is possible to greatly reduce to dynamic
The influence of the free activity of thing.It is used together with microelectrode thruster, is slowly accurately advanced into target brain area.In embedded process, use
Flexible connection mode, separate laser diode-optical fiber-quadrupole pipe electrode coupling body and preamplifier joint.Simultaneously as
Porcelain bushing need not be inserted optical fibers into, thus the stability of neuron activity record is greatly improved.
A kind of embedding method of reusable lightweight fibre electrode, comprises the following steps：
Concentration 1-2% isoflurane anesthesia mouse.Then skull is fixed, and keeps skull horizontal.It is small to remove anesthesia
The calvarium portion hair of mouse, iodophor disinfection skin, then take off iodine with 75% alcohol.Skin and connective tissue are cut, from 1mm before bregma area
Above to cerebellum, at the top of exposure skull.Using 10% hydrogen peroxide Sterile surgery area, then top skull is gently scraped with tweezers, make skull
Rough surface.
Whether horizontal using registration arm right arm detection skull, the difference of the height of bregma and posterior is necessarily less than 200um.Can be anti-
Polyphony school, until horizontal.Afterwards, pilot pin is re-moved to bregma, and the 1.8mm after coordinate is bregma, skull center line are right
Do telltale mark in the site that 1.2mm is opened by side.
The embedding reference electrode in skull area and each 1 of ground wire electrode screw at the top of cerebellum.
Cover ground wire and reference electrode screw exposed portion using Metabond cement, formed 1 be about 12mm, wide 10mm,
High 3mm cement wall.
Square copper mesh is cut into 4, forms 4 equilateral triangle copper mesh, length of side 8cm.Before dental cement wall,
Afterwards, 1 is respectively adhered fixed on left and right wall.
Central point is identified as with positioning, it is 1mm that an area is ground on skull2Bone window, length of side 1mm square window
Mouthful, choose out endocranium, exposure brain tissue.
Under the clamping of registration arm right arm, micro-thruster and optical fiber-electrode coupling body are slowly implanted in brain tissue, initially
Implantation depth 700um.
Fixed using dental cement on micro-thruster and light source-optical fiber-electrode coupling body bottom to dental cement wall.
Neuron is provided into signal record weld on the both sides copper mesh holded up.So, propeller and optical fiber-electrode
Flexible wire electrode is only existed between coupling body and signal record joint to be connected.
Front and rear sides copper mesh on dental cement wall is holded up, and fixation is welded to each other with left and right sides copper mesh, is formed anti-
The only gauze screen of extraneous electric signal interference, the high 25-30mm of gauze screen.
On front side of cement wall copper mesh upper limb inner face weld a 2-8 hole joint, connect laser diode driver line, with to
Laser diode provides dc source.
Appropriate dental cement is smeared into copper mesh bottom surface and musculature adjacent, reduce musculature point activity to cell
The influence of outer tracer signal.
Copper mesh upper end open is sealed using self adhesive bandage, hits or pollute micro-thruster and light to prevent foreign matter from entering
Source-optical fiber-electrode coupling body.
Micro-thruster advances downwardly 70um, examines micro-thruster and optical fiber-electrode coupling body could normal work.
Mouse is put back into raising box, single cage raising, normal drinking-water and feed is given, monitors body weight daily and body is basic
Situation.
Finally it should be noted that：Various embodiments above is merely illustrative of the technical solution of the present invention, rather than its limitations；To the greatest extent
The present invention is described in detail with reference to foregoing embodiments for pipe, it will be understood by those within the art that：Its according to
The technical scheme described in foregoing embodiments can so be modified, either which part or all technical characteristic are entered
Row equivalent substitution；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technology
The scope of scheme, it all should cover among the claim of the present invention and the scope of specification.
Claims (10)
1. a kind of reusable lightweight fibre electrode, it is characterised in that including laser diode, optical fiber, nerve signal
Joint, pcb board, micro-thruster, iron plate, capillary guiding tube, tetrode, connecting rod, the laser diode and optical fiber one end phase
Connection, the nerve signal weld is on pcb board, Electricity Federation between the nerve signal joint and pcb board, the pcb board
It is welded on micro-thruster, the iron plate is vertically fixed on micro-thruster, and the capillary guiding tube is adhered on iron plate, described
Tetrode penetrates capillary guiding tube, and one end of the tetrode protrudes from the length of capillary guiding tube, the tetrode it is another
The hole of end insertion pcb board, described laser diode-optical fiber complex couple with quadrupole pipe electrode through ultraviolet light solidification, institute
State connecting rod one end and be fixed on micro-thruster, the other end of the connecting rod is fixed on laser diode base.
2. a kind of preparation method of reusable lightweight fibre electrode, it is characterised in that comprise the following steps：
S1, laser diode is accurately aligned with optical fiber one end, is bonded using optical fiber glue；
S2, by nerve signal weld on pcb board；
S3, pcb board is welded on micro-thruster, then the perpendicular position of iron plate is fixed on the propeller；
S4, using glue capillary guiding tube is adhered on iron plate；
S5, tetrode is penetrated to capillary guiding tube, one end of tetrode protrudes from the length of capillary guiding tube；
S6, the hole by the other end insertion pcb board of tetrode；
S7, solidify connection laser diode-optical fiber complex and quadrupole pipe electrode using ultraviolet light, reuse connecting rod connect it is micro-
Propeller, one end of connecting rod are fixedly welded on micro-thruster, and the other end is fixed on laser diode base.
3. the preparation method of reusable lightweight fibre electrode according to claim 2, it is characterised in that：It is described
In S1, the concrete mode being bonded using optical fiber glue utilizes purple for optical fiber glue is added between laser diode and optical fiber
Outer photocuring optical fiber glue, is repeated several times, until the fully wrapped around firmly space between laser diode and optical fiber of optical fiber glue.
4. the preparation method of reusable lightweight fibre electrode according to claim 3, it is characterised in that：It is described
In S2, epoxy resin is used to carry out insulation and connection between each welding point afterwards on pcb board nerve signal weld
Solidification.
5. the preparation method of reusable lightweight fibre electrode according to claim 4, it is characterised in that：It is described
In S5, the tetrode is 4, arranged in parallel between the tetrode.
6. the preparation method of reusable lightweight fibre electrode according to claim 5, it is characterised in that：It is described
The specific method of hole of the other end insertion pcb board of tetrode is：The other end of the wire electrode of tetrode is inserted into pcb board
Hole, then with metal pin insertion apertures hole, be stripped off the insulating barrier of wire electrode, so as to establish pcb board epineural active joint with
Electricity contact between wire electrode.
7. the preparation method of reusable lightweight fibre electrode according to claim 6, it is characterised in that：It is described
In S7：Under the microscope, before laser diode-optical fiber complex being parallel into quadrupole pipe electrode, gradually both,
Afterwards, optical fiber glue is added between, by glue curing under ultraviolet light, by laser diode-optical fiber complex
It is coupled with quadrupole pipe electrode, and then laser diode-optical fiber complex is connected with micro-thruster by connecting rod.
A kind of 8. embedding method of reusable lightweight fibre electrode, it is characterised in that：Comprise the following steps：
A, mouse is pre-processed：Using isoflurane anesthesia mouse, its skull is fixed, keeps skull horizontal, removes mouse
Calvarium portion hair, iodophor disinfection skin, then iodine is taken off with 75% alcohol, cut skin and connective tissue, exposure skull top, exposure
Scope from 1mm before bregma area to cerebellum above, using 10% hydrogen peroxide Sterile surgery area, then gently scrape with tweezers top skull, make
Skull surface is coarse；
B, telltale mark：Whether horizontal using registration arm right arm detection skull, the difference of the height of bregma and posterior is necessarily less than
200um, pilot pin is re-moved to bregma, and the 1.8mm after coordinate is bregma, the other position for opening 1.3mm in skull center line right side
Point does telltale mark；
C, the embedding reference electrode in skull area and ground wire electrode screw at the top of cerebellum；
D, ground wire and reference electrode screw exposed portion are covered using cement, forms rectangular dental cement wall；
E, fibre electrode is slowly implanted into brain tissue, is initially implanted into depth 70um, and the fibre electrode bottom of implantation is fixed
On dental cement wall.
F, respectively it is adhered fixed equilateral triangle copper mesh on the front, rear, left and right wall of dental cement wall；
G, neuron is provided into signal record joint and its connecting PCB board is welded on the both sides copper mesh holded up；
H, a joint is welded in dental cement wall side copper mesh upper limb inner face, laser diode driver line is connected, with to laser two
Pole pipe provides dc source.
9. the embedding method of reusable lightweight fibre electrode according to claim 8, it is characterised in that：Also wrap
Include following steps：
I, the front and rear sides copper mesh on dental cement wall is holded up, and fixation is welded to each other with left and right sides copper mesh, formation prevents
The gauze screen of extraneous electric signal interference.
10. the embedding method of reusable lightweight fibre electrode according to claim 9, it is characterised in that：Also
Comprise the following steps：
J, appropriate dental cement is smeared into copper mesh bottom surface and musculature adjacent, reduces musculature point activity to extracellular
The influence of tracer signal；
K, copper mesh upper end open is sealed using self adhesive bandage, hits or pollute fibre electrode to prevent foreign matter from entering；
L, micro-thruster advances downwardly 70um, examines micro-thruster and optical fiber-electrode coupling body could normal work；
M, mouse is put back into raising box, single cage raising.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710675948.0A CN107495963B (en) | 2017-08-09 | 2017-08-09 | Reusable lightweight fibre electrode and preparation method thereof, embedding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710675948.0A CN107495963B (en) | 2017-08-09 | 2017-08-09 | Reusable lightweight fibre electrode and preparation method thereof, embedding method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107495963A true CN107495963A (en) | 2017-12-22 |
| CN107495963B CN107495963B (en) | 2019-07-30 |
Family
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| CN109498990A (en) * | 2018-12-20 | 2019-03-22 | 清华大学 | A kind of fixed device of medical instrument |
| CN107495963B (en) * | 2017-08-09 | 2019-07-30 | 中国人民解放军第三军医大学 | Reusable lightweight fibre electrode and preparation method thereof, embedding method |
| WO2022213655A1 (en) * | 2021-04-08 | 2022-10-13 | 中国科学院深圳先进技术研究院 | Method and device for preparing electrode assembly, photoelectrode device and implantation method therefor |
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| US20130123599A1 (en) * | 2011-11-11 | 2013-05-16 | Korea Institute Of Science And Technology | Micro probe and manufacturing method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107495963B (en) * | 2017-08-09 | 2019-07-30 | 中国人民解放军第三军医大学 | Reusable lightweight fibre electrode and preparation method thereof, embedding method |
| CN109498990A (en) * | 2018-12-20 | 2019-03-22 | 清华大学 | A kind of fixed device of medical instrument |
| WO2022213655A1 (en) * | 2021-04-08 | 2022-10-13 | 中国科学院深圳先进技术研究院 | Method and device for preparing electrode assembly, photoelectrode device and implantation method therefor |
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