CN107494339A - A kind of method by controlling potassium permanganate concentration to improve Tachypleus tridentatus larvae survival rate - Google Patents

A kind of method by controlling potassium permanganate concentration to improve Tachypleus tridentatus larvae survival rate Download PDF

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Publication number
CN107494339A
CN107494339A CN201710818973.XA CN201710818973A CN107494339A CN 107494339 A CN107494339 A CN 107494339A CN 201710818973 A CN201710818973 A CN 201710818973A CN 107494339 A CN107494339 A CN 107494339A
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potassium permanganate
mrow
cataclysm
concentration
permanganate concentration
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廖永岩
刘金霞
关杰耀
方怀义
王鹏良
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Qinzhou University
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Qinzhou University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish

Abstract

The invention belongs to Tachypleus tridentatus larya culturing technical field, discloses a kind of method by controlling potassium permanganate concentration to improve Tachypleus tridentatus larvae survival rate, potassium permanganate safe concentration is 37.7973mg/L；Within cataclysm 96h, below potassium permanganate concentration 200mg/L has no significant effect to survival rate and time-to-live；Potassium permanganate concentration cataclysm 96h, LC50Potassium permanganate concentration is 377.973mg/L；Recover 12h, 24h, 36h, 48h after cataclysm, below cataclysm potassium permanganate concentration 200mg/L returns to 0 not to be influenceed on survival rate；Cataclysm recovers 12h, 24h, and below cataclysm potassium permanganate concentration 500mg/L returns to 0 not to be influenceed on the time-to-live；When cataclysm recovers 36h, 48, below cataclysm potassium permanganate concentration 200mg/L returns to 0mg/L not to be influenceed on the time-to-live.The use of the method, the survival rate of Chinese horseshoe crab nursery and cultivation can be improved.

Description

A kind of method by controlling potassium permanganate concentration to improve Tachypleus tridentatus larvae survival rate
Technical field
The invention belongs to Tachypleus tridentatus larya culturing technical field, more particularly to one kind is by controlling potassium permanganate concentration to improve The method of Tachypleus tridentatus larvae survival rate.
Background technology
Chinese horseshoe crab is called " horseshoe crab fish ", horse crab, category Merostomata arthropod, sword shape tail, whole body yellowish-brown, and whole body is yellowish-brown Color, it is slow in action, all it is hard shell from head to foot, although there is the same chelicera of crab, more the same tails of a sharp sword Bar, generally live in marginal basins.Horseshoe crab is the arthropod breathed with the gill, and horseshoe crab in the world is only left 4 kinds now.This profile The biology of tortoise is somewhat like, is present in the Southern Coast in China, the North Sea is most.On the seashore of Beibu Bay, guangxi, gather in groups band together It is the story that local fisherman continues saying it with interest that horseshoe crab, which climbs to spawning on sandy beach,.Horseshoe crab is a kind of ancient biology, the mud before more than 300,000,000 years Basin is recorded and just lived on earth, and is still kept its form so far, be can be rated as the time immemorial adherents of a former dynasty in ocean, and it and trilobita are the same phases The animal of discipline, therefore have the title of " bioactivation stone ".Tachypleus tridentatus typically lives in abysmal area, and the annual 6-8 months, which are returned on sandy beach, produces Ovum, there is very high requirement to natural environments such as the sandy and temperature at sandy beach.Chinese apus like light and slow current, have it is certain weak Phototaxis, optimum temperature are 15-30 DEG C, and pH scopes are 6-9.It is more suitable under being cultivated with weak direct light (50lex) experimental condition Preferably.Luminous intensity is the seasonal Control factors of the important interior life of horseshoe crab (horse crab) one.Enter after autumn, from shallow sea You Hui deep-seas mistake Winter.Young horseshoe crab grows on beach just migrates shallow sea for 9 years old, typically just to reach sexal maturity by 13 years old, just very resistance to famine after adult, continuously Living on air within 10 months will not also die of hunger, and mainly eat the small shellfish of minibeast, shell, Lingula, sea anemone etc..Usually give birth to Argillo arenaceous seabed living within depth of water 40m, using worm, annelid, brachiopod and mollusk as food, hide by day and come out at night. Larvae being fished for spoon immediately and taking back interior, can be put in experiment basin eat mosquito larvae to observe them, at three days In time, the method that larva is placed into after eating up is taken, interval observes more reduction.In coastal areas of southern China area, horseshoe crab also has One romantic name --- " mandarin duck fish ", because into after the breeding season, male horseshoe crab always tightly embraces female horseshoe crab, and shape shadow is not From.The blood of horseshoe crab is rare blueness, and one touches bacterium and will solidify, and is medically optimal bacterial testing agent. Potassium permanganate is one of envirment factor for influenceing Chinese horseshoe crab survival rate and rate of development, and influence Fish Survival it is important because Element.The toxic action of Environment of Litopenaeus vannamei Low is found by studying several Medicines in Aquaculture such as potassium permanganate, formaldehyde, PVP-I, added After entering medicine, Environment of Litopenaeus vannamei Low starts uneasiness, and acutely travelling, spasmodic tic, body turns white, it is impossible to and it is travelling, until it is dead, its The toxicity of middle potassium permanganate is most strong.Several aquatic products fishing medicines such as potassium permanganate, chlorine dioxide, allicin are studied to blood parrot nursery Acute toxicity test, draw the most strong conclusion of the toxic reaction of potassium permanganate.By analysis of experiments potassium permanganate, BCDMH, Several fishing medicines such as PVP-I, formaldehyde find that potassium permanganate is most strong to the toxicity of freshwater shrimp to the toxic reaction of juvenile freshwater shrimp.It was found that Potassium permanganate generates acute toxicity to Calanus sinicus.Accumulation of the metallic elements such as copper, nickel, zinc in horseshoe crab is studied, so as to understand The pollution condition of different regions.It has studied Various Seasonal, the physiological activity change, migration, mating, spawning etc. of horseshoe crab.Horseshoe crab is because of environment Influence, quantity is constantly reduced, and the province such as Guangdong, Guangxi, Fujian has been included in second class protection animal, China Taiwan Jinmen, Penghu And Hong Kong etc. also set up horseshoe crab protection zone.Medicines in Aquaculture potassium permanganate be influence marine organisms existence a kind of important environment because Son, people to the toxicity research of potassium permanganate by having found that potassium permanganate produces stronger toxic reaction to fish at present.Research hair A kind of Pax genes in existing Chinese horseshoe crab seedling body be present, Pax genes are transcription factors very conservative on a kind of evolve, to tumour The regulating and controlling effect of key is played in the pathological processes such as occurrence and development, the research to cancer has very big realistic meaning.Selenium is people The essential trace element of class, trace elements of selenium and the generation of function of human body and corresponding disease are closely bound up, and China is The country of serious selenium deficiency, the selenium element content in Chinese horseshoe crab body is high, and this is to absorption of the research biology to selenium element, and biology The study on the synthesis of selenoprotein has very high realistic meaning.People increasingly pay attention to the scientific research value of Chinese horseshoe crab at present, and related grinds Study carefully report also to emerge in an endless stream.
In summary, the problem of prior art is present be：Potassium permanganate is a kind of important aquatic products disinfectant；It is but high Influence of the potassium manganate to horseshoe crab, is not studied still.
The content of the invention
The problem of existing for prior art, the invention provides one kind by controlling potassium permanganate concentration to improve Tachypleus tridentatus The method of larvae survival rate.
The present invention is achieved in that a kind of side by controlling potassium permanganate concentration to improve Tachypleus tridentatus larvae survival rate Method, the method by controlling potassium permanganate concentration to improve Tachypleus tridentatus larvae survival rate include：
The safe concentration of potassium permanganate is 37.7973mg/L；
Within cataclysm 96h, below potassium permanganate concentration 200mg/L is not notable to the survival rate of Tachypleus tridentatus and time-to-live Influence；
During potassium permanganate concentration cataclysm 96h, LC50Potassium permanganate concentration is 377.973mg/L；
Recover 12h, 24h, 36h, 48h after cataclysm, below cataclysm potassium permanganate concentration 200mg/L returns to potassium permanganate Concentration 0 has no significant effect to the survival rate of Chinese horseshoe crab；
When cataclysm recovers 12h, 24h, below cataclysm potassium permanganate concentration 500mg/L returns to potassium permanganate concentration 0mg/L The time-to-live of Chinese horseshoe crab is had no significant effect；
When cataclysm recovers 36h, 48, below cataclysm potassium permanganate concentration 200mg/L returns to 0mg/L pairs of potassium permanganate concentration The time-to-live of Chinese horseshoe crab has no significant effect.
Further, the method by controlling potassium permanganate concentration to improve Tachypleus tridentatus larvae survival rate uses potassium permanganate Recover experiment after concentration cataclysm experiment, potassium permanganate concentration cataclysm and SPSS 17.0 carries out variance analysis test its significant difference Property；Multiple range test between carrying out group with the new multipole difference method of inspection of LSR methods；With Probit methods obtain 12h, 24h, 36h, 48h, 60h, 72h, 84h, 96h LC50Potassium permanganate concentration.
Further, it is described that survival rate public affairs are calculated by the method for controlling potassium permanganate concentration to improve Tachypleus tridentatus larvae survival rate Formula is as follows：
S：Survival rate；a：Survival seedling number in each container；b：Each container seedling sum；
Different time sections time-to-live calculation formula is as follows：
Wherein, h1, h2, h3, h4, h5, h6, h7 represent that seedling is deposited when 24h, 36h, 48h, 60h, 72h, 84h, 96h respectively Live time, a1, a2, a3, a4, a5, a6, a7, a8 represent respectively seedling survival 12h, 24h, 36h, 48h, 60h, 72h, 84h, 96h number.
Further, it is described by controlling under the method different time sections that potassium permanganate concentration improves Tachypleus tridentatus larvae survival rate The cataclysm of potassium permanganate concentration is to the virulence regression equation of Chinese horseshoe crab seedling：
48h y=3.714-0.004x；
60h y=2.738-0.004x；
72h y=2.359-0.004x；
84h y=3.045-0.007x；
96h y=4.078-0.011x.
Advantages of the present invention and good effect are：Since concentration 0mg/L, potassium permanganate cataclysm is carried out to Chinese horseshoe crab seedling The experiment of Survival Effects, as a result finds, right within potassium permanganate concentration 650mg/L within 48h during potassium permanganate concentration cataclysm The survival rate of Chinese horseshoe crab and time-to-live have no significant effect (p ＜ 0.05)；Within cataclysm 96h, potassium permanganate concentration 200mg/ Below L has no significant effect (p ＜ 0.05) to the survival rate of Tachypleus tridentatus and time-to-live, is Chinese horseshoe crab potassium permanganate concentration cataclysm Normal survival concentration；LC during potassium permanganate concentration cataclysm 96h50Potassium permanganate concentration is 377.973mg/L, be Chinese horseshoe crab can Survival concentration.Recover 12h, 24h, 36h, 48h after cataclysm, below cataclysm potassium permanganate concentration 200mg/L returns to potassium permanganate Concentration 0 has no significant effect (p ＜ 0.05) to the survival rate of Chinese horseshoe crab；When cataclysm recovers 12h, 24h, cataclysm potassium permanganate concentration Below 500mg/L returns to potassium permanganate concentration 0mg/L and the time-to-live of Chinese horseshoe crab had no significant effect (p ＜ 0.05).Suddenly When becoming recovery 36h, 48, below cataclysm potassium permanganate concentration 200mg/L returns to potassium permanganate concentration 0mg/L to Chinese horseshoe crab Time-to-live has no significant effect (p ＜ 0.05).The present invention carries out acute toxicity test of the potassium permanganate to Tachypleus tridentatus, in understanding Magnificent horseshoe crab provides scientific basis, preferably " biology is living for protection to the adaptability of potassium permanganate concentration for the monitoring of Chinese horseshoe crab living environment Fossil ".
Brief description of the drawings
Fig. 1 is the method provided in an embodiment of the present invention by controlling potassium permanganate concentration to improve Tachypleus tridentatus larvae survival rate Flow chart.
Fig. 2 is influence signal of the potassium permanganate concentration cataclysm provided in an embodiment of the present invention to Chinese horseshoe crab survival rate of seedling Figure.
Fig. 3 is the LC of potassium permanganate concentration cataclysm China provided in an embodiment of the present invention horseshoe crab seedling50Concentration schematic diagram.
Fig. 4 is influence signal of the potassium permanganate concentration cataclysm provided in an embodiment of the present invention to the Chinese horseshoe crab seedling survival time Figure.
Fig. 5 is influence of the recovery to Chinese horseshoe crab survival rate of seedling after potassium permanganate concentration cataclysm provided in an embodiment of the present invention Schematic diagram.
Fig. 6 is to recover the shadow to the Chinese horseshoe crab seedling survival time after potassium permanganate concentration cataclysm provided in an embodiment of the present invention Ring schematic diagram.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to Limit the present invention.
The application principle of the present invention is explained in detail below in conjunction with the accompanying drawings.
It is as shown in figure 1, provided in an embodiment of the present invention by controlling potassium permanganate concentration to improve Tachypleus tridentatus larvae survival rate Method comprise the following steps：
S101：In the seawater that salinity is 25 add potassium permanganate mother liquor be configured to concentration be 0mg/L, 50mg/L, 200mg/L, 350mg/L, 500mg/L, 650mg/L, 800mg/L totally 7 gradients (0 is control group)；It is with specification per gradient 13.6cm × 13.6cm × 19cm, capacity are about 4L containers, the depth of water that is filled with water 10cm, and setting 3 is parallel；To be 25 in salinity Seawater in temporary foster 3d Chinese horseshoe crab, direct plunge into each potassium permanganate concentration gradient container, every container is put in a suitable place to breed in 10 Magnificent horseshoe crab seedling；This experiment carries out 4d, and per 12h, observation once, records its survival rate, time-to-live and behavior dynamic；
S102：After the completion of 4d potassium permanganate concentration cataclysm experiment, the Chinese horseshoe crab seedling that each group is survived directly moves into The seawater that salinity is 25 carries out recovery experiment, and Chinese horseshoe crab survival test is carried out by potassium permanganate concentration cataclysm pattern.This test into Row 2d, per 12h, observation once, records its survival rate, time-to-live and behavior dynamic；
S103：Variance analysis (One-way ANOVA) is carried out using SPSS 17.0 and examines its significance of difference；With LSR methods Newly again range test (SSR inspections) method (Duncan methods) carry out Multiple range test between group；p<0.05 is significant difference；Use Probit Method obtains 12h, 24h, 36h, 48h, 60h, 72h, 84h, 96h LC50Potassium permanganate concentration；
S104：Calculate survival rate, different time sections time-to-live.
The application principle of the present invention is further described with reference to experiment.
1 materials and methods
1.1 material
1.1.1 Chinese horseshoe crab seedling
Experiment is bought in Zhanjiang artificial breeding field with Chinese horseshoe crab seedling, belongs to the first phase young, average to nearly weigh 0.025g greatly, Tested after temporarily supporting 3d in the seawater of salinity 25.
1.1.2 seawater
Seawater is the natural sea-water through sand filter, then filters standby, salinity 25 through 400 mesh sieve tulles.
1.2 method
1.2.1 the cataclysm of potassium permanganate concentration is tested
In the seawater that salinity is 25 add potassium permanganate mother liquor be configured to concentration be 0mg/L, 50mg/L, 200mg/L, 350mg/L, 500mg/L, 650mg/L, 800mg/L totally 7 gradients (0 is control group).Per gradient with specification be 13.6cm × 13.6cm × 19cm, capacity are about 4L containers, the depth of water that is filled with water 10cm, and setting 3 is parallel.In the seawater that will be 25 in salinity Temporarily foster 3d Chinese horseshoe crab, direct plunges into each potassium permanganate concentration gradient container, every container puts 10 Chinese horseshoe crab seedling in a suitable place to breed. This experiment carries out 4d, and per 12h, observation once, records its survival rate, time-to-live and behavior dynamic.
1.2.2 experiment is recovered after the cataclysm of potassium permanganate concentration
After the completion of 4d potassium permanganate concentration cataclysm experiment, Chinese horseshoe crab seedling that each group is survived directly moves into salinity and is 25 seawater carries out recovery experiment, and Chinese horseshoe crab survival test is carried out by potassium permanganate concentration cataclysm pattern.This experiment carries out 2d, often 12h is observed once, records its survival rate, time-to-live and behavior dynamic.
1.2.3 statistical analysis
Variance analysis (One-way ANOVA) is carried out using SPSS 17.0 and examines its significance of difference；It is new with LSR methods Multiple range test (SSR inspections) method (Duncan methods) carries out Multiple range test between group；p<0.05 is significant difference；Asked with Probit methods Go out 12h, 24h, 36h, 48h, 60h, 72h, 84h, 96h LC50Potassium permanganate concentration.
1.2.4 computational methods
Survival rate calculation formula is as follows：
S：Survival rate；a：Survival seedling number in each container；b：Each container seedling sum.
Different time sections time-to-live calculation formula is as follows：
Wherein, h1, h2, h3, h4, h5, h6, h7 represent that seedling is deposited when 24h, 36h, 48h, 60h, 72h, 84h, 96h respectively Live time, a1, a2, a3, a4, a5, a6, a7, a8 represent respectively seedling survival 12h, 24h, 36h, 48h, 60h, 72h, 84h, 96h number.
2 results and analysis
Influence of the 2.1 potassium permanganate concentration cataclysms to Chinese horseshoe crab seedling
Influence of the potassium permanganate concentration cataclysm to Chinese horseshoe crab seedling, including potassium permanganate concentration to Chinese horseshoe crab survival rate, deposit The influence of live time.
2.1.1 influence of the potassium permanganate concentration cataclysm to Chinese horseshoe crab survival rate of seedling
Influence of the potassium permanganate concentration cataclysm to the survival rate of Chinese horseshoe crab seedling is obvious, the results are shown in Table 1.
The survival rate of the potassium permanganate concentration cataclysm different time sections China horseshoe crab seedling of table 1
Fig. 2 is shown in data progress One-way ANOVA statistical analyses to table 1.
As it is clear from fig. 2 that in cataclysm 12h, 24h, 36h, the cataclysm of potassium permanganate concentration does not have to the survival rate of Chinese horseshoe crab seedling Have a significant impact (p ＞ 0.05)：In cataclysm 12,24,36h hours, the survival rate within concentration 800mg/L is 100%, Illustrate in a short time, below concentration 800mg/L does not influence on the survival rate of Chinese horseshoe crab seedling.In cataclysm 36h, permanganic acid Potassium concn cataclysm has on the survival rate of Chinese horseshoe crab seedling to be significantly affected (p ＜ 0.05)：Below cataclysm concentration 500mg/L and cataclysm Concentration 650mg/L groups significant difference (p ＜ 0.05), illustrates, during cataclysm 36h, below concentration 500mg/L deposits to Chinese horseshoe crab seedling Motility rate influence is not notable, and concentration 650mg/L produces toxicity to Chinese horseshoe crab seedling, is not suitable for survival.During cataclysm 48h, concentration centering The survival rate of magnificent horseshoe crab seedling, which has, significantly affects (F(8,18)=57.875, p ＜ 0.05)：Cataclysm concentration 200mg/L is poor between the following group Different not notable (p ＞ 0.05), cataclysm concentration 200mg/L and concentration 350mg/L above group difference significantly (F(8,18)=57.875, P ＜ 0.05), this illustrates, within cataclysm 48h, survival of the potassium permanganate concentration cataclysm to below 200mg/L to Chinese horseshoe crab seedling Rate influence is not notable, can survive, and more than cataclysm concentration 350mg/L and concentration 200mg/L group differences are notable (p ＜ 0.05), Illustrate more than concentration 350mg/L to be not suitable for Chinese horseshoe crab seedling survival.During cataclysm 96h, concentration has to the survival rate of Chinese horseshoe crab seedling Have a significant impact (p ＜ 0.05)：The following group differences of cataclysm concentration 200mg/L are not notable (p ＞ 0.05), cataclysm concentration More than 350mg/L and concentration 200mg/L group differences significantly (p ＜ 0.05), illustrate more than concentration 350mg/L to be not suitable for China Horseshoe crab seedling survival.
2.1.2 Probit is analyzed
With Probit regression analyses, the data of table 1 are handled, draw potassium permanganate concentration cataclysm in different time sections Under virulence regression equation, the results are shown in Table 2.
Virulence regression equation of the potassium permanganate concentration cataclysm to Chinese horseshoe crab seedling under the different time sections of table 2
LC under potassium permanganate concentration cataclysm different time sections50As a result Fig. 3 is seen.
As seen from Figure 3, during cataclysm 12h, 24h, 36h, Chinese horseshoe crab has no dead within concentration 800mg/L, does not produce toxicity. LC during cataclysm 48h50Concentration is higher, but with the increase of cataclysm time, LC50Salinity gradually reduces, during cataclysm 96h, LC50Concentration For 377.973mg/L.Illustrate that Chinese horseshoe crab is higher to the tolerance of potassium permanganate concentration, can be deposited under higher potassium permanganate concentration It is living, when potassium permanganate concentration is more than 377.973mg/L, the survival rate of Chinese horseshoe crab seedling can be had an impact.
2.1.3 influence of the potassium permanganate concentration cataclysm to the Chinese horseshoe crab time-to-live
Influence of the potassium permanganate concentration cataclysm to the time-to-live of Chinese horseshoe crab is observed, the results are shown in Table 3.
The time-to-live of the potassium permanganate concentration cataclysm different time sections China horseshoe crab seedling of table 3
Data progress One-way ANOVA statistic analysis results to table 3 are shown in Fig. 4.
As can be seen from Figure 4, during cataclysm 12h, 24h, 36h, group difference is not notable (p ＞ 0.05) within concentration 800mg/L, Potassium permanganate concentration does not influence on the time-to-live of Chinese horseshoe crab seedling, and potassium permanganate concentration is to China within the 36h short time Horseshoe crab seedling does not produce lethal toxicity, can survive.During cataclysm 48h, time-to-live of the potassium permanganate concentration to Chinese horseshoe crab seedling Have a significant impact, not significantly (p ＞ 0.05), cataclysm potassium permanganate is dense for the following group differences of cataclysm potassium permanganate concentration 500mg/L Spend 650mg/L and cataclysm potassium permanganate concentration 500mg/L group differences significantly (p ＜ 0.05), this explanation, during cataclysm 48h, suddenly Become below potassium permanganate concentration 500mg/L influences not notable, cataclysm potassium permanganate concentration to the time-to-live of Chinese horseshoe crab seedling More than 500mg/L produces toxicity to Chinese horseshoe crab seedling, is unfavorable for the survival of Chinese horseshoe crab.During cataclysm 60h, potassium permanganate concentration centering The time-to-live of magnificent horseshoe crab, which has, to be significantly affected (p ＜ 0.05)：The following group differences of cataclysm potassium permanganate concentration 200mg/L are not notable (p ＞ 0.05), but, this illustrates with cataclysm concentration 200mg/L above group difference significantly (p ＜ 0.05), it is high within cataclysm 60h Time-to-live influences of below the mangaic acid potassium concn 200mg/L on Chinese horseshoe crab seedling be not notable, during more than concentration 200mg/L is not suitable for Magnificent horseshoe crab seedling survival.During cataclysm 96h, potassium permanganate concentration has on the time-to-live of Chinese horseshoe crab to be significantly affected (p ＜ 0.05)： The following group differences of cataclysm potassium permanganate concentration 200mg/L not significantly (p ＞ 0.05), cataclysm potassium permanganate concentration 200mg/L with Upper group difference significantly (p ＜ 0.05), illustrates within potassium permanganate concentration cataclysm 96h, below potassium permanganate concentration 200mg/L is right The time-to-live of seedling has no significant effect, and potassium permanganate concentration is then not suitable for seedling survival more than 200mg/L.
Recover the influence to Chinese horseshoe crab seedling after 2.2 potassium permanganate concentration cataclysms
2.2.1 the influence to Chinese horseshoe crab survival rate of seedling is recovered after the cataclysm of potassium permanganate concentration
After potassium permanganate concentration returns to 0mg/L, the survival rate of Chinese horseshoe crab seedling different time sections is shown in Table 5.
Recover the survival rate of different time sections China horseshoe crab seedling after the potassium permanganate concentration cataclysm of table 5
Statistical analysis such as Fig. 6 is carried out to the data of table 5
Found from Fig. 6, when recovering 12h, 24h, 36h, 48h, below potassium permanganate concentration 200mg/L returns to concentration 0mg/L group differences are not significantly (p ＞ 0.05), but more than potassium permanganate concentration 200mg/l returns to concentration 0mg/L significant differences (p ＜ 0.05) then illustrates, potassium permanganate concentration cataclysm to below 200mg/L, the vigor of Chinese horseshoe crab can be repaired, and not influence Its survival rate.After concentration is more than 200mg/L, potassium permanganate concentration produces certain lethal toxicity to Chinese horseshoe crab and is difficult to recover, And the irrecoverability of toxicity increases with the increase of concentration, show as the death rate and increase with the increase of concentration.
2.2.2 the influence to the Chinese horseshoe crab seedling survival time is recovered after the cataclysm of potassium permanganate concentration
After potassium permanganate concentration tests recover, the time-to-live of Chinese horseshoe crab seedling different time sections is shown in Table 6.
Recover the time-to-live of different time sections China horseshoe crab seedling after the potassium permanganate concentration cataclysm of table 6
When recovering 12h, 24h, below cataclysm potassium permanganate concentration 500mg/L returns to poor between 0 group of potassium permanganate concentration Different not notable (p ＞ 0.05), when this explanation recovers 12h, 24h, it is dense that below potassium permanganate concentration 500mg/L returns to potassium permanganate It is not notable to spend time-to-live influences of the 0mg/L on Chinese horseshoe crab seedling.When recovering 36h, 48h, potassium permanganate concentration 200mg/L Return to below potassium permanganate concentration 0 time-to-live of Chinese horseshoe crab seedling is influenceed it is not notable, potassium permanganate concentration 350mg/L and 500mg/L returns to time-to-live significant difference (p ＞ 0.05) of the potassium permanganate concentration 0 to Chinese horseshoe crab seedling.
3 discuss
3.1 result
Recover survival rate to Chinese horseshoe crab seedling, the shadow of time-to-live after the cataclysm of potassium permanganate concentration, cataclysm by studying Ring, it can be deduced that following conclusion：
Within potassium permanganate concentration cataclysm 36h, potassium permanganate concentration is not notable to Chinese horseshoe crab survival rate and time-to-live Influence；Cataclysm 96h, below potassium permanganate concentration 200mg/L have no significant effect to Chinese horseshoe crab survival rate and time-to-live, in being Magnificent horseshoe crab normally survives concentration；
During cataclysm 96h, LC50Potassium permanganate concentration is 377.973mg/L, is illustrated, during below salinity 377.973mg/L is Magnificent horseshoe crab can survive concentration；
When recovering 12h, 24h, 36h, 48h after potassium permanganate concentration cataclysm, below potassium permanganate concentration 200mg/L is returned to Potassium permanganate concentration 0mg/L has no significant effect to Chinese horseshoe crab survival rate；
When recovering 12h, 24h, 36h, 48h after potassium permanganate concentration cataclysm, below potassium permanganate concentration 200mg/L is returned to Potassium permanganate concentration 0mg/L has no significant effect to the Chinese horseshoe crab time-to-live；
The potassium permanganate concentration tolerance of 3.2 Tachypleus tridentatus seedling
In this experiment, the cataclysm of potassium permanganate concentration produces significant shadow to the survival rate of Chinese horseshoe crab seedling and time-to-live Ring (p ＜ 0.05), potassium permanganate concentration rise, the survival rate of Chinese horseshoe crab seedling reduces, and the time-to-live is short.Experiment proof, Gao Meng Sour potassium concn cataclysm is to 500mg/L, the survival rate only up to 13% of Chinese horseshoe crab seedling, time-to-live 69.2h.Cataclysm is to potassium permanganate More than concentration 650mg/L (including 650mg/L), survival rate 0, time-to-live not more than 61.6h.And potassium permanganate concentration When cataclysm is to 50mg/L~350mg/L, the survival rate of Chinese horseshoe crab seedling is higher, up to more than 50%, more than time-to-live 82h, and Probit analyses are drawn, during cataclysm 96h, LC50Potassium permanganate concentration is 377.973mg/L, then illustrates potassium permanganate concentration Below 377.973mg/L is the concentration of surviving of Chinese horseshoe crab seedling, and 200mg/L is safe concentration.It can be seen that compare other water Generation, Chinese horseshoe crab are eager to excel more to the adaptability of potassium permanganate, but also have the limit, the Chinese horseshoe crabs of below 200mg/L can be just Often existence, 200mg/L to 377.973mg/L are the concentration of surviving of Chinese horseshoe crab, are difficult to more than 377.973mg/L China horseshoe crab Existence.
Recover the influence to Tachypleus tridentatus after 3.3 potassium permanganate concentration cataclysms
When recovering 12h, 24h, 36h, 48h after potassium permanganate concentration cataclysm, below potassium permanganate concentration 200mg/L is returned to Potassium permanganate concentration 0mg/L has no significant effect (p ＞ 0.05) to Chinese horseshoe crab survival rate.Recover experiment and have no dead, explanation Poisoning is lighter, is also not reaching to the recovery limit of Chinese horseshoe crab, can be survived after being transferred to safe concentration.Recover experiment There are Chinese horseshoe crab death of seedling, and increasing over time in succession because toxicity is heavier during 200mg/L-50mg/L, in recovery process Increase greatly.When recovering 12h, 24h, 36h, 48h after potassium permanganate concentration cataclysm, below potassium permanganate concentration 200mg/L recovers The Chinese horseshoe crab time-to-live is had no significant effect (p ＞ 0.05) to potassium permanganate concentration 0mg/L.It can be seen that in concentration 200mg/L Following recovery experiment, the survival rate of Chinese horseshoe crab horseshoe crab seedling is not influenceed by the time, shallower due to being poisoned, normal dense when returning to , will not be dead again after degree.And when potassium permanganate cataclysm is 200mg/L-500mg/L, recover experiment has horseshoe crab seedling dead in succession To die, mean survival time is also being reduced, and as the time of the recovery Average Survival of the increase horseshoe crab seedling of concentration constantly reduces.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention All any modification, equivalent and improvement made within refreshing and principle etc., should be included in the scope of the protection.

Claims (4)

  1. A kind of 1. method by controlling potassium permanganate concentration to improve Tachypleus tridentatus larvae survival rate, it is characterised in that described to pass through The method that control potassium permanganate concentration improves Tachypleus tridentatus larvae survival rate includes：
    The safe concentration of potassium permanganate is 37.7973mg/L；
    Within cataclysm 96h, below potassium permanganate concentration 200mg/L does not have notable shadow to the survival rate of Tachypleus tridentatus and time-to-live Ring；
    During potassium permanganate concentration cataclysm 96h, LC50Potassium permanganate concentration is 377.973mg/L；
    Recover 12h, 24h, 36h, 48h after cataclysm, below cataclysm potassium permanganate concentration 200mg/L returns to potassium permanganate concentration 0 The survival rate of Chinese horseshoe crab is had no significant effect；
    When cataclysm recovers 12h, 24h, below cataclysm potassium permanganate concentration 500mg/L returns to potassium permanganate concentration 0mg/L centerings The time-to-live of magnificent horseshoe crab has no significant effect；
    When cataclysm recovers 36h, 48, below cataclysm potassium permanganate concentration 200mg/L returns to potassium permanganate concentration 0mg/L to China The time-to-live of horseshoe crab has no significant effect.
  2. 2. the method by controlling potassium permanganate concentration to improve Tachypleus tridentatus larvae survival rate as claimed in claim 1, its feature It is, it is described by controlling the method that potassium permanganate concentration improves Tachypleus tridentatus larvae survival rate to be tried using the cataclysm of potassium permanganate concentration Test, recover experiment after potassium permanganate concentration cataclysm and SPSS 17.0 carries out variance analysis test its significance of difference；With LSR methods New multipole difference method of inspection carry out Multiple range test between group；96h LC of the potassium permanganate to the Tachypleus tridentatus young is obtained with Probit methods50 For 377.973mg/L.
  3. 3. the method by controlling potassium permanganate concentration to improve Tachypleus tridentatus larvae survival rate as claimed in claim 1, its feature It is, it is described as follows by the method calculating survival rate formula for controlling potassium permanganate concentration to improve Tachypleus tridentatus larvae survival rate：
    <mrow> <mi>S</mi> <mo>=</mo> <mfrac> <mi>a</mi> <mi>b</mi> </mfrac> <mo>;</mo> </mrow>
    S：Survival rate；a：Survival seedling number in each container；b：Each container seedling sum；
    Different time sections time-to-live calculation formula is as follows：
    <mrow> <mi>h</mi> <mn>1</mn> <mo>=</mo> <mfrac> <mrow> <mn>12</mn> <mi>a</mi> <mn>1</mn> <mo>+</mo> <mn>24</mn> <mi>a</mi> <mn>2</mn> </mrow> <mn>10</mn> </mfrac> <mo>;</mo> </mrow>
    <mrow> <mi>h</mi> <mn>2</mn> <mo>=</mo> <mfrac> <mrow> <mn>12</mn> <mi>a</mi> <mn>1</mn> <mo>+</mo> <mn>24</mn> <mi>a</mi> <mn>2</mn> <mo>+</mo> <mn>36</mn> <mi>a</mi> <mn>3</mn> </mrow> <mn>10</mn> </mfrac> <mo>;</mo> </mrow>
    <mrow> <mi>h</mi> <mn>3</mn> <mo>=</mo> <mfrac> <mrow> <mn>12</mn> <mi>a</mi> <mn>1</mn> <mo>+</mo> <mn>24</mn> <mi>a</mi> <mn>2</mn> <mo>+</mo> <mn>36</mn> <mi>a</mi> <mn>3</mn> <mo>+</mo> <mn>48</mn> <mi>a</mi> <mn>4</mn> </mrow> <mn>10</mn> </mfrac> <mo>;</mo> </mrow>
    <mrow> <mi>h</mi> <mn>4</mn> <mo>=</mo> <mfrac> <mrow> <mn>12</mn> <mi>a</mi> <mn>1</mn> <mo>+</mo> <mn>24</mn> <mi>a</mi> <mn>2</mn> <mo>+</mo> <mn>36</mn> <mi>a</mi> <mn>3</mn> <mo>+</mo> <mn>48</mn> <mi>a</mi> <mn>4</mn> <mo>+</mo> <mn>60</mn> <mi>a</mi> <mn>5</mn> </mrow> <mn>10</mn> </mfrac> <mo>;</mo> </mrow>
    <mrow> <mi>h</mi> <mn>5</mn> <mo>=</mo> <mfrac> <mrow> <mn>12</mn> <mi>a</mi> <mn>1</mn> <mo>+</mo> <mn>24</mn> <mi>a</mi> <mn>2</mn> <mo>+</mo> <mn>36</mn> <mi>a</mi> <mn>3</mn> <mo>+</mo> <mn>48</mn> <mi>a</mi> <mn>4</mn> <mo>+</mo> <mn>60</mn> <mi>a</mi> <mn>5</mn> <mo>+</mo> <mn>72</mn> <mi>a</mi> <mn>6</mn> </mrow> <mn>10</mn> </mfrac> <mo>;</mo> </mrow>
    <mrow> <mi>h</mi> <mn>6</mn> <mo>=</mo> <mfrac> <mrow> <mn>12</mn> <mi>a</mi> <mn>1</mn> <mo>+</mo> <mn>24</mn> <mi>a</mi> <mn>2</mn> <mo>+</mo> <mn>36</mn> <mi>a</mi> <mn>3</mn> <mo>+</mo> <mn>48</mn> <mi>a</mi> <mn>4</mn> <mo>+</mo> <mn>60</mn> <mi>a</mi> <mn>5</mn> <mo>+</mo> <mn>72</mn> <mi>a</mi> <mn>6</mn> <mo>+</mo> <mn>84</mn> <mi>a</mi> <mn>7</mn> </mrow> <mn>10</mn> </mfrac> <mo>;</mo> </mrow>
    <mrow> <mi>h</mi> <mn>7</mn> <mo>=</mo> <mfrac> <mrow> <mn>12</mn> <mi>a</mi> <mn>1</mn> <mo>+</mo> <mn>24</mn> <mi>a</mi> <mn>2</mn> <mo>+</mo> <mn>36</mn> <mi>a</mi> <mn>3</mn> <mo>+</mo> <mn>48</mn> <mi>a</mi> <mn>4</mn> <mo>+</mo> <mn>60</mn> <mi>a</mi> <mn>5</mn> <mo>+</mo> <mn>72</mn> <mi>a</mi> <mn>6</mn> <mo>+</mo> <mn>84</mn> <mi>a</mi> <mn>7</mn> <mo>+</mo> <mn>96</mn> <mi>a</mi> <mn>8</mn> </mrow> <mn>10</mn> </mfrac> <mo>;</mo> </mrow>
    Wherein, when h1, h2, h3, h4, h5, h6, h7 represent seedling survival when 24h, 36h, 48h, 60h, 72h, 84h, 96h respectively Between, a1, a2, a3, a4, a5, a6, a7, a8 represent seedling survival 12h, 24h, 36h, 48h, 60h, 72h, 84h, 96h respectively Number.
  4. 4. the method by controlling potassium permanganate concentration to improve Tachypleus tridentatus larvae survival rate as claimed in claim 1, its feature It is, potassium permanganate is dense under the method different time sections by controlling potassium permanganate concentration raising Tachypleus tridentatus larvae survival rate Spend cataclysm is to the virulence regression equation of Chinese horseshoe crab seedling：
    48h y=3.714-0.004x；
    60h y=2.738-0.004x；
    72h y=2.359-0.004x；
    84h y=3.045-0.007x；
    96h y=4.078-0.011x.
CN201710818973.XA 2017-09-12 2017-09-12 A kind of method by controlling potassium permanganate concentration to improve Tachypleus tridentatus larvae survival rate Pending CN107494339A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111665209A (en) * 2020-07-24 2020-09-15 北部湾大学 Chinese horseshoe crab blood quality monitoring method based on continuous blood collection

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111665209A (en) * 2020-07-24 2020-09-15 北部湾大学 Chinese horseshoe crab blood quality monitoring method based on continuous blood collection
CN111665209B (en) * 2020-07-24 2021-12-21 北部湾大学 Chinese horseshoe crab blood quality monitoring method based on continuous blood collection

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Application publication date: 20171222