CN107485463A - A kind of method for building Parkinsonian rats using unilateral 2 injections - Google Patents
A kind of method for building Parkinsonian rats using unilateral 2 injections Download PDFInfo
- Publication number
- CN107485463A CN107485463A CN201710653745.1A CN201710653745A CN107485463A CN 107485463 A CN107485463 A CN 107485463A CN 201710653745 A CN201710653745 A CN 201710653745A CN 107485463 A CN107485463 A CN 107485463A
- Authority
- CN
- China
- Prior art keywords
- injections
- rats
- unilateral
- building
- rat
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D1/00—Surgical instruments for veterinary use
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Surgery (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of method for building Parkinsonian rats using unilateral 2 injections, this method comprises the following steps：Step 1, after rats by intraperitoneal injection is anaesthetized, overhead hair is cut off, cut scalp, hypodermis and periosteum；Step 2, the skull carefully drilled through using dental burr or syringe needle at positioning not damage endocranium, and the micro syringe of Sucked medicine is connected on micro propeller by the coordinate determined, the μ l of 6 OHDA 5 configured to SNc and VTA injections, vertically enter cranium；After step 3, suture, the continuous intraperitoneal injection unit of penicillin 50,000 builds animal model to prevent and treat infection within three days；Step 4, the circling behavior of APO inductions rat to the left is injected intraperitoneally after 10 days.The method of the present invention punctures Rat calvarial during modelling using syringe needle can effectively save the modelling time, convenient and swift, and the requirement to instrument is simple simultaneously can be more sterile.
Description
Technical field
The invention belongs to biological technical field, is related to a kind of unilateral 2 injections of use and builds Parkinsonian rats
Method.
Background technology
Parkinson's (Parkinson disease, PD) are used as neurodegenerative diseases, its typical pathological characters
Reduced for substantia nigra of midbrain dopamine (dopamine DA) serotonergic neuron missing and nigro-striatal pathway DA quantity.Parkinson
The method for building up of animal model has a variety of, at present prepared by most commonly 6-OHDA (6 hydroxyl dopamine) rat model and 1-
Methyl 4-phenyl -1,2,3,6 tetrahydropyridines (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine,
MPTP the mouse) made or primate model.It is generally in the majority to select rat to make animal model.The PD that 6-OHDA induces
Animal model has very big similitude in terms of neural biochemical, pathology and behaviouristics with mankind PD, has been widely used for grinding for PD
Study carefully.However, the degree and feature of 6-0HDA induction PD neurodegenerations are substantially influenceed mouth by injection site.6-OHDA is built
It is more at present using substantia nigra compacta (SNc), Ventral Midbrain covered (VTA), corpus straitum and medial forebrain bundle to make PD rat models
(medial forebrain bundle MFB) single-point or two point injection damage PD models to make unilateral or bilateral.SNc areas
Domain is small, and general modeling success rate is low.VTA is closely related as another important target spot and somatic movement, relevant with PD pathology
Important area, if individually damage, also occurs the low situation of modeling rate.Damage method is combined with VTA two point using SNc, can
So that damage range is larger, shorten the modeling cycle, effectively improve the rat that modeling success rate establishes stable simulation PD middle and advanced stages
Model, laboratory basic research platform is provided for the research treatment of disease.
The content of the invention
It is an object of the invention to provide a kind of method for building Parkinsonian rats using unilateral 2 injections.
Its concrete technical scheme is：
A kind of method for building Parkinsonian rats using unilateral 2 injections, comprises the following steps：
Step 1, after rats by intraperitoneal injection is anaesthetized, overhead hair is cut off, is fixed on stereotaxic apparatus, bilateral ear
Bar tip insertion external auditory meatus, makes head fix keep level, and forward and backward fontanel is kept on same plane as far as possible, iodine disinfection, cuts
Start skin, hypodermis and periosteum；
Step 2, the skull carefully drilled through using dental burr or syringe needle at positioning not damage endocranium, by determination
Right side, SNc：5.4 ± 0.1mm after bregma, 1.4 ± 0.1mm on the right side of sagittal suture, 7.8 ± 0.1mm under endocranium；VTA：After bregma
5.0 ± 0.1mm, 0.9 ± 0.1mm on the right side of center line, 7.2 ± 0.1mm under endocranium, the coordinate determined is by the micro of Sucked medicine
Syringe is connected on micro propeller, to the SNc and VTA μ l of 6-OHDA 5 that have configured of injection, vertically enters cranium, slow inserting needle,
It is 1 μ l/min that injection, which keeps speed, let the acupuncture needle remain at a certain point 10min, slow withdraw of the needle 1mm/min, observation rat vital sign in art；
After step 3, suture, the continuous intraperitoneal injection unit of penicillin 50,000 builds animal model to prevent and treat infection within three days；
Step 4, the circling behavior of APO inductions rat to the left is injected intraperitoneally after 10 days, 10min rats just go out after injection
Existing behaviouristics changes, and continuously records 40min：Majority is using left rear limb as fulcrum during rotation, and body ring song is in " C " shape, head and the tail phase
Connect, smell sample action of looking for food with visiting, some rotary speeies are too fast or even overturn.
Further, in step 2, described syringe needle is No. 4 syringe needles.
Further, in step 2, described 6-OHDA concentration is 2 μ g/ μ l.
Compared with prior art, beneficial effects of the present invention are：
The method of the present invention punctures Rat calvarial during modelling using syringe needle can effectively save modelling
Time, while the too deep damage for causing brain tissue of the penetration depth caused by dynamics is excessive can be reduced, it is convenient and swift, to instrument
It is required that simple simultaneously can be more sterile.On the other hand, the point methods of one side two of use prepare P of Rats D models, can be from pathology base
Plinth imitates PD generation, more conforms to the pathogenesis of this book of disease, can more shorten the modelling time, so as to improve model
It is ageing, while also can effectively ensure that success rate.It is adapted to the research on PD bases.
Embodiment
Technical scheme is described in more detail with reference to specific embodiment.
After rats by intraperitoneal injection is anaesthetized, overhead hair is cut off, is fixed on stereotaxic apparatus (bilateral ear bar tip
External auditory meatus is inserted, head is fixed keep level, and forward and backward fontanel is kept on same plane as far as possible).Iodine disinfection, cut head
Skin, hypodermis and periosteum.(seminar is adopted as No. 4 syringe needles and replaces dental burr generally use dental burr, because of dentistry
Bore on the one hand operation and need to be grasped certain skill, while also easily cause because being difficult to control using dynamics, cause easily damage hard
Meninx.The stability in site can be improved when rotating and puncture using syringe needle, while it is about 0.5mm to assess needle diameter, operation side
Just it is easily mastered).The careful skull drilled through at positioning not damage endocranium, with reference to the chief editors' such as Paxinos《Rat brain is three-dimensional
Position collection of illustrative plates》.By determination right side, SNc：After bregma (5.4 ± 0.1mm), on the right side of sagittal suture (1.4 ± 0.1mm), under endocranium
(7.8±0.1mm)；VTA：After bregma (5.0 ± 0.1mm), on the right side of center line (0.9 ± 0.1mm), under endocranium (7.2 ± 0.1)
mm.The micro syringe of Sucked medicine is connected on micro propeller by the coordinate determined, is configured to SNc and VTA injections
6-OHDA (2 μ g/ μ l) 5 μ l, vertically enter cranium, slow inserting needle, it is 1 μ l/min that injection, which keeps speed, let the acupuncture needle remain at a certain point 10min, is slowly moved back
Pin 1mm/min, rat vital sign is observed in art.Conventional suture, the continuous intraperitoneal injection unit of penicillin 50,000 is felt with preventing and treating within three days
Dye.Build animal model.The circling behavior that APO induces rat (strong side) to the left is injected intraperitoneally after 10 days.10min after injection
Left and right rat just may occur in which that behaviouristics changes, and continuously record 40min：For majority using left rear limb as fulcrum, body ring is bent during rotation
It is end to end in " C " shape, sample action of looking for food is smelt with visiting, some rotary speeies are too fast or even overturn.Modeling success rate is reachable
To 40% or so.And pass through the circling behavior and pathology of animal, the biochemical success that can be able to verify model.
The foregoing is only a preferred embodiment of the present invention, protection scope of the present invention not limited to this, any ripe
Those skilled in the art are known in the technical scope of present disclosure, the letter for the technical scheme that can be become apparent to
Altered or equivalence replacement are each fallen within protection scope of the present invention.
Claims (3)
- A kind of 1. method for building Parkinsonian rats using unilateral 2 injections, it is characterised in that comprise the following steps：Step 1, after rats by intraperitoneal injection is anaesthetized, overhead hair is cut off, is fixed on stereotaxic apparatus, bilateral ear bar point End insertion external auditory meatus, makes head fix keep level, and forward and backward fontanel is kept on same plane as far as possible, iodine disinfection, cuts head Skin, hypodermis and periosteum；Step 2, the skull carefully drilled through using dental burr or syringe needle at positioning not damage endocranium, right by determining Side, SNc：5.4 ± 0.1mm after bregma, 1.4 ± 0.1mm on the right side of sagittal suture, 7.8 ± 0.1mm under endocranium；VTA：5.0 after bregma ± 0.1mm, 0.9 ± 0.1mm on the right side of center line, 7.2 ± 0.1mm under endocranium, the coordinate determined is by the micro note of Sucked medicine Emitter is connected on micro propeller, the μ l of 6-OHDA 5 configured to SNc and VTA injections, vertically enters cranium, slow inserting needle, note It is 1 μ l/min to penetrate and keep speed, let the acupuncture needle remain at a certain point 10min, slow withdraw of the needle 1mm/min, observation rat vital sign in art；After step 3, suture, the continuous intraperitoneal injection unit of penicillin 50,000 builds animal model to prevent and treat infection within three days；Step 4, the circling behavior of APO inductions rat to the left is injected intraperitoneally after 10 days, 10min rats occur as soon as row after injection Change to learn, continuously record 40min：Majority is using left rear limb as fulcrum during rotation, and body ring song is in " C " shape, end to end, companion Sample action of looking for food is smelt in spy, and some rotary speeies are too fast or even overturn.
- 2. the method according to claim 1 for building Parkinsonian rats using unilateral 2 injections, its feature exist In in step 2, described syringe needle is No. 4 syringe needles.
- 3. the method according to claim 1 for building Parkinsonian rats using unilateral 2 injections, its feature exist In in step 2, described 6-OHDA concentration is 2 μ g/ μ l.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710653745.1A CN107485463A (en) | 2017-07-19 | 2017-07-19 | A kind of method for building Parkinsonian rats using unilateral 2 injections |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710653745.1A CN107485463A (en) | 2017-07-19 | 2017-07-19 | A kind of method for building Parkinsonian rats using unilateral 2 injections |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107485463A true CN107485463A (en) | 2017-12-19 |
Family
ID=60645272
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710653745.1A Pending CN107485463A (en) | 2017-07-19 | 2017-07-19 | A kind of method for building Parkinsonian rats using unilateral 2 injections |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107485463A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110269040A (en) * | 2019-05-28 | 2019-09-24 | 军事科学院军事医学研究院环境医学与作业医学研究所 | A kind of method for building up of particulate matter breath exposure neurotoxicity rat model |
| CN110327132A (en) * | 2019-08-19 | 2019-10-15 | 四川农业大学 | A kind of easy sturgeon telocoele medication |
| CN113229212A (en) * | 2021-05-12 | 2021-08-10 | 山东大学深圳研究院 | Method for establishing rat Parkinson disease model by double-site combined injection of 6-OHDA and alpha-synuclein |
-
2017
- 2017-07-19 CN CN201710653745.1A patent/CN107485463A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110269040A (en) * | 2019-05-28 | 2019-09-24 | 军事科学院军事医学研究院环境医学与作业医学研究所 | A kind of method for building up of particulate matter breath exposure neurotoxicity rat model |
| CN110327132A (en) * | 2019-08-19 | 2019-10-15 | 四川农业大学 | A kind of easy sturgeon telocoele medication |
| CN113229212A (en) * | 2021-05-12 | 2021-08-10 | 山东大学深圳研究院 | Method for establishing rat Parkinson disease model by double-site combined injection of 6-OHDA and alpha-synuclein |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107485463A (en) | A kind of method for building Parkinsonian rats using unilateral 2 injections | |
| CN106511385A (en) | Medicine for treating hair loss caused by chemotherapy | |
| Mahoney et al. | Psychosurgery: history of the neurosurgical management of psychiatric disorders | |
| KR102531586B1 (en) | Device for inserting a blunt-tip cannula under the skin of a patient | |
| US4620540A (en) | Mold for rapid stereotaxic injections into mouse striatum | |
| CN207384278U (en) | Digital imagery hair follicle stem cells transplant semi-automatic implanting device | |
| EP4279581A1 (en) | Technical method for treating leucoderma using hair follicle melanocyte stem cell transplantation | |
| Li et al. | An automatic drug injection device with spatial micro-force perception guided by an microscopic image for robot-assisted ophthalmic surgery | |
| CN212322511U (en) | Integrated model for lower dental canal treatment and micro apical operation | |
| CN212112949U (en) | Upper tooth root canal therapy and micro apical operation integrated model | |
| ZA200209847B (en) | Novel needle for medical device and device comprising same. | |
| CN113229212B (en) | Method for establishing rat Parkinson disease model by double-site combined injection of 6-OHDA and alpha-synuclein | |
| CN203576920U (en) | Needle and thread integrated acupoint catgut embedding micro-needle | |
| CN106730308A (en) | A kind of fibroin albumen micropin of imitative mosquito mouthpart and preparation method thereof | |
| Chung et al. | Analysis of the clinical and aesthetic results of facial dimple creation surgery | |
| RU2426563C2 (en) | Method of conductor anesthesia | |
| RU78657U1 (en) | DEVICE FOR TRAINING NEURAL SURGEON DOCTORS WITH ENDOSCOPIC MANUAL SKILLS OF OPERATIVE INTERVENTIONS AT CLINICAL HEMATOMAS IN THE PROJECTION OF THE ANTERIAL CRANULAR FOSSAL | |
| CN110279685A (en) | Serine is preventing or is treating the application in the craniocerebral injury of plateau | |
| CN200960290Y (en) | Disposable acupuncture and moxibustion instrument | |
| CN203749980U (en) | Puncturing guide and local anesthetizing needle head | |
| CN205411918U (en) | Slowly -releasing needling instrument | |
| Elliott | A reliable, rapid and inexpensive method for producing and implanting chronic cannulae into brains of small animals | |
| ATE355083T1 (en) | POLYACRYLAMIDE GEL KIT FOR THE PRODUCTION OF A CAPSULE IN THE TISSUE OF A MAMMAL ORGANISM AND ANIMAL ALLOGENE OR XENOGENE CELLS, FOR THE TREATMENT OF ONCOLOGICAL DISEASES AND DIABETES MELLITUS | |
| CN104114111A (en) | Methods and apparatuses for harvesting, modifying and reimplantation of dermal micro -organs | |
| CN201139680Y (en) | Olecranon type needle for foreign body on cornea |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171219 |
|
| RJ01 | Rejection of invention patent application after publication |