CN107478707A - A kind of novel electrode and its assay method for blood sugar detection - Google Patents
A kind of novel electrode and its assay method for blood sugar detection Download PDFInfo
- Publication number
- CN107478707A CN107478707A CN201710568928.3A CN201710568928A CN107478707A CN 107478707 A CN107478707 A CN 107478707A CN 201710568928 A CN201710568928 A CN 201710568928A CN 107478707 A CN107478707 A CN 107478707A
- Authority
- CN
- China
- Prior art keywords
- electrode
- modified
- cnt
- glucose
- carbon
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/308—Electrodes, e.g. test electrodes; Half-cells at least partially made of carbon
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention relates to electrochemical analysis detection technique field, more particularly to a kind of novel electrode and its assay method available for blood sugar detection, including being working electrode by glass-carbon electrode or modified electrode, saturated calomel electrode is reference electrode, platinum filament for auxiliary electrode group into three-electrode system, modified electrode is the CNT/palladium nanostructure composite material prepared using electrodeposition process, it is an object of the invention to use the nano combined modified electrode of CNT/palladium, establish a kind of new method for determining blood glucose, study electrochemical behavior of the blood glucose on the modified electrode, this method has higher selectivity to glucose, higher sensitivity, and it is easier to operate.
Description
Technical field
The present invention relates to electrochemical analysis detection technique field, and in particular to a kind of novel electrode available for blood sugar detection
And its assay method.
Background technology
Glucose, it is the important raw material of industry, is mainly used in food industry, such as produces candy, cake, beverage.Curing
Treatment field, glucose are widely applied in the transfusion of patient.Its oxidized product is gluconic acid, is to produce medicine of replenishing the calcium
The important source material of thing-calcium gluconate.Positive D-mannitol can be generated by being reduced, and can be used to synthesize vitamin C.At present, people couple
Substantial amounts of research has been done in the detection of glucose, have also been developed the detection method of a variety of glucose, mainly have chromatography,
Spectroscopic methodology, bioanalysis and electrochemical process.Because electrochemical process detects glucose compared to other method stability, sensitivity is all
Have greatly improved, so it receives great attention in glucose detection field, achieve quick development.
The content of the invention
It is an object of the invention to use CNT/palladium nanostructure composite material modified glassy carbon electrode, a kind of survey is established
Determine the new method of blood glucose, electrochemical behavior of the research blood glucose on the modified electrode, a kind of new point is provided for the measure of blood glucose
Analysis method.
To achieve the above object, the technical solution adopted by the present invention is：Including being work by glass-carbon electrode or modified electrode
Electrode, saturated calomel electrode are reference electrode, platinum filament for auxiliary electrode group into three-electrode system.Modified electrode is carbon nanometer
Pipe/palladium nanostructure composite material.The three-electrode system is placed in the serum handled well, in -0.8~0.4V potential ranges it
Between with 100mV/s sweep speed under the conditions of, record peak current is that cyclic voltammetric in the range of 0.06~2.0 mg/mL is bent in concentration
Line.Specific operating procedure includes：
1）The preparation of sensor：The pretreatment of glass-carbon electrode, the purifying of CNT and carboxylated, electro-deposition prepare carbon nanometer
Pipe/palladium nano structural composite electrode material；
2）The optimization of condition determination：The species of cushioning liquid, PH influence, the influence of sedimentation time, the influence of sweep speed；
3）The measure of actual sample：Working electrode is glass-carbon electrode（GCE）, reference electrode is saturated calomel electrode（SCE）, to electricity
Extremely platinum electrode, form three-electrode system；The three-electrode system is placed in the serum handled well, remembered with optimum experimental condition
It is the cyclic voltammetry curve in the range of 0.06~2.0 mg/mL that peak current, which is recorded, in concentration.
The present invention has good electric conductivity using glass-carbon electrode, and combines CNT, the property of palladium bichloride, is made one
Kind has highly sensitive electrode to glucose.Then by optimizing sedimentation time, buffer solution species, sweep speed, bottom liquid pH
Etc. parameter, obtain that there is higher selectivity, higher sensitivity and the electrochemical analysis side for being easier operation to glucose
Method, and this method is determined applied to actual sample.
Brief description of the drawings
The present invention is further detailed explanation with specific embodiment below in conjunction with the accompanying drawings.
Accompanying drawing 1 is the sulfuric acid activated figure of bare electrode.
Accompanying drawing 2 is cyclic voltammogram of the Different electrodes in potassium ferricyanide solution.
Accompanying drawing 3 is cyclic voltammetry curve of the Different electrodes in 0.5mg/mL glucose solution.
Accompanying drawing 4 is PH Optimal Curve.
Accompanying drawing 5 is the Optimal Curve of sedimentation time.
Accompanying drawing 6 is the Optimal Curve of sweep speed.
Accompanying drawing 7 is schemed for the CV of different glucose solution.
Accompanying drawing 8 is the glucose solution I-V Characteristic Analysis figure with CNT/palladium bichloride modified electrode various concentrations
Reduction peak, the canonical plotting made.
Embodiment
To a kind of novel electrode for blood sugar detection involved in the present invention and utilize the electrode pair referring to the drawings
The electrochemical method that blood glucose is measured is described in detail.
To realize the purpose of invention, the technical solution adopted by the present invention is：Including being work by glass-carbon electrode or modified electrode
Make electrode, saturated calomel electrode is reference electrode, platinum filament for auxiliary electrode group into three-electrode system.Modified electrode is carbon nanometer
Pipe/palladium nanostructure composite material.
The pretreatment of glass electrode：Glass-carbon electrode is taken in abrasive paper for metallograph（3000#）Upper polishing, then respectively in dry, wet muntjac
Successively with 1.0,0.3 and 0.05 μm of Al on leather packing2O3Powder polishes, successively with deionized-distilled water, absolute ethyl alcohol, deionization
Distilled water is fully cleaned by ultrasonic, then electrode is placed in 0.5mol/L H2SO4In solution, with 50mV/s sweep speeds, -0.5
~+1.4V potential regions, use cyclic voltammetry（CV）Scanning 20 circle, will be sulfuric acid activated after electrode take out after use distilled water flushing
Totally, it is placed in 0.01g/mL K3[Fe(CN)6In solution, with 50mV/s sweep speeds, in -0.5~+1.2V potential regions,
Use cyclic voltammetry（CV）Scanning is until stable.
The purifying of CNT and carboxylated：In the preparation process of CNT, simultaneous amorphous carbon, carbon are received
The generation of the amorphous carbon such as rice grain and carbon fiber, to eliminate as much as the influence of impurity band, before CNT carboxylated
Purification processes need to be carried out to it.CNT heats 2h before use in 400 DEG C, to remove amorphous carbon thereon, CNT
Carboxylated uses nitration mixture reflux oxidation method, i.e., by CNT in V (HNO3):V(H2SO4)=1:It is heated to reflux in 3 mixed liquor
6h, cooled and filtered, with ultra-pure water eccentric cleaning to neutrality, it is put into baking oven and dries.It is configured to 0.5mg/mL CNTs point
Dispersion liquid is stand-by.
Electro-deposition prepares CNT/palladium nano structural composite electrode material：Electro-deposition is prepared by CHI660 electrochemistry
Work station is carried out, using three-electrode system：Glass-carbon electrode（GCE）For working electrode, platinum filament is to electrode, saturated calomel electrode
（SCE）For reference electrode.Take appropriate CNT disperse drop dried under the surface of glass-carbon electrode, infrared lamp i.e. be made carbon receive
Mitron modified electrode.This step is due to the reason that CNT has very strong electric conductivity so that the response signal of modified electrode
Become apparent from.Carbon nano tube modified modified electrode is placed in 0.5mmol/L palladium chloride solution, in -0.2V potentiostatic electrodepositions
180s, Technique of Nano Pd/carbon nano tube modified electrode is made.
Accompanying drawing 1 is the sulfuric acid activated figure of bare electrode.Glass-carbon electrode is taken in abrasive paper for metallograph（3000#）Upper polishing, then exists respectively
Successively with 1.0,0.3 and 0.05 μm of Al in dry, wet chamois leather pad2O3Powder polish, successively with deionized-distilled water, absolute ethyl alcohol,
Deionized-distilled water is fully cleaned by ultrasonic, then electrode is placed in 0.5mol/L H2SO4In solution, with 50mV/s sweep speeds,
In -0.5~+1.4V potential regions, cyclic voltammetry is used（CV）The circle of scanning 20.Electrode after will be sulfuric acid activated uses distillation after taking out
Water is rinsed well, is placed in 0.01g/mL K3[Fe(CN)6] in solution, with 50mV/s sweep speeds, in -0.5~+1.2V electricity
Position section, uses cyclic voltammetry（CV）Scanning is until stable.
Accompanying drawing 2 is cyclic voltammogram of the Different electrodes in potassium ferricyanide solution.Successively with glass-carbon electrode, CNT
K of modified electrode, CNT/the palladium bichloride modified electrode in 5mmol/L3[Fe(CN)6] scanned using cyclic voltammetry in solution
（Potential range -0.4~0.8V, sweep speed 50mV/s）.In figure, carbon nano tube modified electrode（Curve c）Compared with glass-carbon electrode（It is bent
Line b）Oxidation peak current increase, reduction peak current reduce, show that CNT has been aggregated to GCE surfaces, promote probe ion
In the redox reaction of electrode surface.And when modifying palladium bichloride in carbon nano tube surface（Curve a）, CNT/chlorination
Redox peaks on palladium modified electrode more highlight, and peak current also further enhances, and illustrate that palladium bichloride clearly enhances electrode
Electric conductivity so that electron surface electronics circulating rate significantly increases, so as to promote probe molecule K3[Fe(CN)6In electrode table
The reversible reaction in face.
Accompanying drawing 3 is cyclic voltammetry curve of the Different electrodes in 0.5mg/mL glucose solution.Delayed with the PBS of pH=6.8
The concentration for rushing solution preparation is 0.5mg/mL glucose solution, and glass-carbon electrode, carbon nano tube modified electrode, palladium bichloride is modified electric
Pole, CNT/palladium bichloride modified electrode between -0.8~0.4V potential ranges with 100mV/s sweep speed under the conditions of, it is different
The cyclic voltammetry scan of electrode scanning, occurs on CNT/palladium bichloride modified electrode and carbon nano tube modified electrode reversible
Oxidation peak and reduction peak, the electrode process for showing glucose is reversible.Simultaneously it is also seen that CNT/chlorination
Palladium modified electrode is remarkably reinforced to the electrocatalytic effect of glucose, shows that response of this modified electrode to glucose is maximum.
Accompanying drawing 4 is PH optimal conditions.The PBS that this experiment employs pH=6.7 contains as bottom liquid progress glucose
The measure of amount.
Accompanying drawing 5 is the Optimal Curve of sedimentation time.When being 3min between when deposited, catalytic current will appear from maximum.
Accompanying drawing 6 is the Optimal Curve of sweep speed.When surface sweeping speed is 100mV/s, peak current is maximum.
Accompanying drawing 7 is schemed for the CV of different glucose solution.Preparing solution concentration respectively with the PBS cushioning liquid of pH=7 is
0.06mg/mL, 0.5mg/mL, 1.0mg/mL, 1.5mg/mL, 2.0mg/mL glucose solution, under optimum experimental condition, use
Cyclic voltammetry carries out modified electrode measure to the glucose solution of various concentrations.
Accompanying drawing 8 is standard curve.With the glucose solution C-V characteristic of CNT/palladium bichloride modified electrode various concentrations
The reduction peak of analysis chart, and using Excel softwares, make standard curve.
The present invention has good electric conductivity using glass-carbon electrode, and combines CNT, the property of palladium bichloride, is made one
Kind has highly sensitive electrode to glucose.Then by optimizing sedimentation time, buffer solution species, sweep speed, bottom liquid pH
Etc. parameter, obtain that there is higher selectivity, higher sensitivity and the electrochemical analysis side for being easier operation to glucose
Method.
Claims (3)
- A kind of 1. novel electrode for blood sugar detection, it is characterised in that including：Glass-carbon electrode or modified electrode are work electricity Pole, saturated calomel electrode are reference electrode, platinum filament for auxiliary electrode group into three-electrode system.
- 2. a kind of novel electrode for blood sugar detection as claimed in claim 1, it is characterised in that modified electrode is carbon nanometer Pipe/palladium nanostructure composite material.
- 3. a kind of a kind of novel electrode for blood sugar detection using described in claim 1 determines the method for blood glucose, its feature It is, the three-electrode system is placed in the serum handled well, speed is swept with 100mV/s between -0.8~0.4V potential ranges Under the conditions of, record peak current is in the cyclic voltammetry curve that concentration is in the range of 0.06~2.0 mg/mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710568928.3A CN107478707A (en) | 2017-07-13 | 2017-07-13 | A kind of novel electrode and its assay method for blood sugar detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710568928.3A CN107478707A (en) | 2017-07-13 | 2017-07-13 | A kind of novel electrode and its assay method for blood sugar detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107478707A true CN107478707A (en) | 2017-12-15 |
Family
ID=60595508
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710568928.3A Pending CN107478707A (en) | 2017-07-13 | 2017-07-13 | A kind of novel electrode and its assay method for blood sugar detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107478707A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102507693A (en) * | 2011-11-03 | 2012-06-20 | 桂林医学院 | Functional-material-based glucose biosensor and manufacturing method thereof |
| CN102520035A (en) * | 2011-11-04 | 2012-06-27 | 上海大学 | Preparation method for copper oxide-graphene nano-complex modification electrode, and application of modification electrode in glucose detection |
| CN104918925A (en) * | 2012-12-21 | 2015-09-16 | 美艾利尔瑞士公司 | Test device for electrochemical analysis |
-
2017
- 2017-07-13 CN CN201710568928.3A patent/CN107478707A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102507693A (en) * | 2011-11-03 | 2012-06-20 | 桂林医学院 | Functional-material-based glucose biosensor and manufacturing method thereof |
| CN102520035A (en) * | 2011-11-04 | 2012-06-27 | 上海大学 | Preparation method for copper oxide-graphene nano-complex modification electrode, and application of modification electrode in glucose detection |
| CN104918925A (en) * | 2012-12-21 | 2015-09-16 | 美艾利尔瑞士公司 | Test device for electrochemical analysis |
Non-Patent Citations (3)
| Title |
|---|
| LING MENG 等: "Nonenzymatic Electrochemical Detection of Glucose Based on Palladium-Single-Walled Carbon Nanotube Hybrid Nanostructures", 《ANALYTICAL CHEMISTRY》 * |
| SAN HUA LIM 等: "A glucose biosensor based on electrodeposition of palladium nanoparticles and glucose oxidase onto Nafion-solubilized carbon nanotube electrode", 《BIOSENSORS AND BIOELECTRONICS》 * |
| 王荣海 等: "钯复合材料电极催化甲醇氧化机理", 《工业催化》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ratautaite et al. | Molecularly imprinted polypyrrole based sensor for the detection of SARS-CoV-2 spike glycoprotein | |
| Xiao et al. | Characterization of hydrophobic ionic liquid-carbon nanotubes–gold nanoparticles composite film coated electrode and the simultaneous voltammetric determination of guanine and adenine | |
| Khan et al. | Electrochemical determination of uric acid in the presence of ascorbic acid on electrochemically reduced graphene oxide modified electrode | |
| CN105776183B (en) | A kind of preparation method and applications of ferrocenyl carbon nano tube compound material | |
| Tashkhourian et al. | Simultaneous determination of tyrosine and tryptophan by mesoporous silica nanoparticles modified carbon paste electrode using H-point standard addition method | |
| Wang et al. | Application of a single-wall carbon nano-tube film electrode to the determination of trace amounts of folic acid | |
| CN102636540A (en) | Glucose detection sensor, and preparation and application methods thereof | |
| CN108061750B (en) | Electrochemical biosensor constructed based on protein-like nanowires with electrocatalytic activity and used for detecting hydrogen peroxide and glucose | |
| Salimi et al. | Carbon Nanotubes‐Ionic Liquid and Chloropromazine Modified Electrode for Determination of NADH and Fabrication of Ethanol Biosensor | |
| Ba et al. | Determination of l-tryptophan in the presence of ascorbic acid and dopamine using poly (sulfosalicylic acid) modified glassy carbon electrode | |
| CN102175736A (en) | Enzyme electrode for detecting sterigmatocystin and preparation and application thereof | |
| CN109613081A (en) | A kind of detection junket amine molecule electrochemical sensor and its preparation method and application | |
| CN106896147B (en) | A kind of electrochemical sensor of Rapid Determination of Plasma indoles | |
| CN103645237A (en) | Electrochemical sensor for detecting content of carbendazim in fog drops as well as preparation and application methods | |
| CN106053564B (en) | A kind of method that graphite-phase nitrogen carbide-chitosan-modified electrode detects protocatechuic acid as working electrode | |
| CN109085225A (en) | A kind of preparation method of the protein electrochemistry trace sensor of step sedimentation modification carbon electrode | |
| Yang et al. | CTAB functionalized graphene oxide/multiwalled carbon nanotube composite modified electrode for the simultaneous determination of sunset yellow and tartrazine | |
| Hadi et al. | Sensitive detection of histamine at metal-organic framework (Ni-BTC) crystals and multi-walled carbon nanotubes modified glassy carbon electrode | |
| Hasoň et al. | Label-free electrochemical analysis of purine nucleotides and nucleobases at disposable carbon electrodes in microliter volumes | |
| CN102692435B (en) | A kind of assay method of 1, the 8-diaminonaphthalene based on electrochemical DNA biosensor | |
| Dehdashtian et al. | Fabrication of a novel electrochemical sensor based on an electrosynthesized indolyldihydroxyquinone as a bio-based modifier for sensitive and selective direct electrochemical determination of tryptophan | |
| CN109900760B (en) | Preparation method and application of polyacid-based dopamine electrochemical sensor | |
| Ding et al. | Direct electrochemical response of cytochrome c on a room temperature ionic liquid, N-butylpyridinium tetrafluoroborate, modified electrode | |
| CN104316580B (en) | A kind of method of nanogold enzyme sensor detection water nitrite | |
| CN101726521B (en) | Biosensor for rapidly detecting sterigmatocystin and assembling method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20171215 |