CN107475173B - Bacillus stearothermophilus spore culture auxiliary, culture medium and application - Google Patents
Bacillus stearothermophilus spore culture auxiliary, culture medium and application Download PDFInfo
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Abstract
The bacillus stearothermophilus spore culture auxiliary agent provided by the invention comprises 1-20 g of fructose, 0.1-10 g of D L-tryptophan, 0.01-5 g of glutathione and 0.1-10 mg of p-aminobenzoic acid per 100m L, and fructose, D L-tryptophan, glutathione and p-aminobenzoic acid contained in the culture auxiliary agent can synergistically promote germination of bacillus stearothermophilus spores, improve the germination rate, shorten the germination time of the bacillus stearothermophilus spores and improve the working efficiency when the bacillus stearothermophilus spores are used as standard strains.
Description
Technical Field
The invention relates to the technical field of microbial culture media, in particular to a bacillus stearothermophilus spore culture auxiliary agent, a culture medium and application.
Background
Bacillus stearothermophilus (Bacillus stearothermophilus) belongs to thermophilic aerobic Bacillus, is facultative anaerobic, and has bacterial propagules with blue-blue staining in purple and bacterial spores capable of staining with malachite green. The bacterial propagules have low requirements on a culture medium, grow well on bromocresol purple glucose peptone agar, and have rough and beige surfaces. The minimum growth temperature is 28 ℃, and the maximum growth temperature is 70-77 ℃. The optimal growth and propagation temperature is 56-65 ℃, colonies can be formed after culturing for 24h, and no colonies can be seen after culturing for 24h at 37 ℃. The growth is good in a culture medium with the pH value of 6.8-7.2, and the growth cannot be realized when the pH value is close to 5.
Spores produced by the bacillus stearothermophilus are nontoxic and nonpathogenic, have strong resistance to heat, radioactive rays and chemical substances, and particularly have stronger resistance to pressure steam and hydrogen peroxide than most microorganisms, so the bacillus stearothermophilus is used as a standard strain for thermal sterilization and low-temperature plasma sterilization of hydrogen peroxide in China, Europe, America and other areas. In addition, Bacillus stearothermophilus was also used as a standard strain for detecting antibiotic residues.
The bacillus stearothermophilus comprises two forms of propagules and spores. When the Bacillus stearothermophilus is used as a standard strain for detection, spore germination and growth are required to reach a thallus reproduction stage, namely, to reach a propagule. Traditionally, bromocresol purple grape glycoprotein peptone agar is a characteristic culture medium of a propagule thereof, and there is no research report specially aiming at a spore germination growth culture medium.
Disclosure of Invention
In view of the above, it is necessary to provide a bacillus stearothermophilus spore culture auxiliary agent, a culture medium and an application thereof, aiming at the problems that when spore culture is carried out by using a bromocresol purple glucose peptone agar medium, the culture period is long, and the detection efficiency by using bacillus stearothermophilus is low.
The bacillus stearothermophilus spore culture auxiliary agent provided by the invention comprises 1-20 g of fructose, 0.1-10 g of D L-tryptophan, 0.01-5 g of glutathione and 0.1-10 mg of p-aminobenzoic acid per 100m L.
In one embodiment, the culture aid comprises 0.1-10 g of xanthan gum per 100m L.
In one embodiment, the culture aid comprises 0.1-10 g of beef powder per 100m L.
In one embodiment, the culture aid is filtered through a sterile filter.
The invention also provides application of the culture aid, wherein the culture aid is added into a culture medium for culturing the bacillus stearothermophilus.
The invention also provides a bacillus stearothermophilus spore culture medium which comprises a culture medium body and the culture auxiliary agent, wherein every 1000m L of the culture medium body contains 5-20 g of peptone, 1-20 g of glucose and 0.3-1 m L of bromocresol purple ethanol solution.
In one embodiment, the culture auxiliary agent is 1-20 m L per 1000m L of the culture medium.
In one embodiment, the culture medium contains 3.0-20 g of agar per 1000m L.
In one embodiment, the culture aid is added to the culture medium before the culture medium is used.
The invention also provides an application of the bacillus stearothermophilus spore culture medium, and the culture medium is applied to antibiotic residue detection or disinfection and sterilization effect evaluation.
According to the bacillus stearothermophilus spore culture auxiliary agent, fructose, D L-tryptophan, glutathione and p-aminobenzoic acid contained in the culture auxiliary agent can synergistically promote germination of bacillus stearothermophilus spores to be accelerated, the germination rate is improved, the germination time of the bacillus stearothermophilus spores is shortened, and the working efficiency when the bacillus stearothermophilus spores are used as standard strains is improved.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the Bacillus stearothermophilus spore culture auxiliary, culture medium and application of the present invention are further described in detail by the following examples. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The bacillus stearothermophilus spore culture auxiliary agent provided by the invention contains 1-20 g of fructose, 0.1-10 g of D L-tryptophan, 0.01-5 g of glutathione and 0.1-10 mg of p-aminobenzoic acid per 100m of L culture auxiliary agents.
The fructose, D L-tryptophan, glutathione and p-aminobenzoic acid contained in the bacillus stearothermophilus spore culture auxiliary agent can synergistically promote germination of bacillus stearothermophilus spores to be accelerated, improve the germination rate, shorten the germination time of the bacillus stearothermophilus spores and improve the working efficiency when the bacillus stearothermophilus spores are used as standard strains.
Furthermore, the culture auxiliary agent is added with fructose, D L-tryptophan, glutathione and p-aminobenzoic acid according to the proportion, so that germination of bacillus stearothermophilus spores can be better promoted among the components, and the influence on germination efficiency of the bacillus stearothermophilus spores caused by excessive or insufficient amount of one component is avoided.
Furthermore, each 100m L culture aid contains 0.1-10 g of xanthan gum, and by adding the xanthan gum, the viscosity and sensory permeability of the culture medium can be increased, the interpretation efficiency of detection results is improved, and the interference of background color or impurities of detection substances is effectively avoided.
Furthermore, each 100m of L culture auxiliary agent contains 0.1-10 g of beef powder, and the beef powder can supplement and enrich amino acids and minerals required by germination of bacillus stearothermophilus spores, and can further improve the germination rate of the bacillus stearothermophilus spores.
Further, the culture aid is filtered through a sterile filter. Through the filtration treatment of the sterile filter, the culture auxiliary agent can be sterile, and the influence of introducing mixed bacteria on the germination of bacillus stearothermophilus spores and the detection can be avoided. Furthermore, the sterile filter is adopted for filtration treatment, so that the components in the culture auxiliary agent can be prevented from being damaged by high-temperature and high-pressure sterilization, and the components can better perform synergistic action.
The invention also provides an application of the culture aid, and the culture aid is added into a culture medium for culturing the bacillus stearothermophilus. The culture aid provided by the invention is directly added into the existing culture medium for culturing the bacillus stearothermophilus, the existing culture medium is not required to be improved, the existing culture medium can be utilized, the germination efficiency of bacillus stearothermophilus spores in the culture medium is accelerated, and the culture time is shortened.
The invention also provides a bacillus stearothermophilus spore culture medium which comprises a culture medium body and the culture auxiliary agent, wherein every 1000m L of the culture medium body contains 5-20 g of peptone, 1-20 g of glucose and 0.3-1 m L of bromocresol purple ethanol solution.
The bacillus stearothermophilus spore culture medium contains the culture auxiliary agent, and can utilize fructose, D L-tryptophan, glutathione and aminobenzoic acid in the culture auxiliary agent to synergistically promote the germination of bacillus stearothermophilus spores, improve the germination efficiency, shorten the germination time of the bacillus stearothermophilus spores and improve the working efficiency when the bacillus stearothermophilus spores are used as standard strains.
Alternatively, the bromocresol purple ethanol solution has a bromocresol purple concentration of 2%.
Furthermore, the culture auxiliary agent is 1-20 m L per 1000m L of the culture medium, the culture auxiliary agent is added in an amount which is 0.1-2% of the total amount of the culture medium, the culture auxiliary agent can play a role in improving germination efficiency, the using amount of the culture auxiliary agent is small, and the cost of the culture medium is hardly increased while the germination efficiency is promoted.
Furthermore, every 1000m L of the culture medium contains 3.0-20 g of agar, and the culture medium can be made into a solid state by adding a proper amount of agar so as to meet the requirement of the solid culture medium.
Further, the culture auxiliary is added to the culture medium before the culture medium is used. The culture aid is added into the culture medium and mixed before use, so that the failure of effective components of the culture aid caused by premature addition of the culture aid is avoided, and the culture medium can be sterilized by a low-cost and convenient high-temperature and high-pressure method for use. For example, the culture medium may be sterilized by autoclaving at 115 ℃ for 30min before use, and the culture medium may be supplemented with a culture aid treated with a sterile filter to ensure sterility of the resulting culture medium.
The invention also provides an application of the bacillus stearothermophilus spore culture medium, and the culture medium is applied to antibiotic residue detection or disinfection and sterilization effect evaluation.
In the detection of the residual antibiotics in fresh milk, the application principle of the bacillus stearothermophilus spore culture medium is as follows:
after a culture medium containing bacillus stearothermophilus spores and bromcresol purple indicators is added into a sample to be detected and incubated at 65 ℃, if the sample does not contain antibiotics or the concentration of the antibiotics is lower than the detection limit, the spores grow in the culture medium and produce acid, and the color of the pH indicator is changed into yellow. Conversely, if the sample contains an antibiotic above the detection limit, spores will not grow and the color of the pH indicator remains purple.
In the evaluation of disinfection and sterilization effects, the application principle of the bacillus stearothermophilus spore culture medium is as follows: the bacillus stearothermophilus spores are high-temperature resistant, and can be killed when the sterilization condition reaches 121 ℃ for 20 min. If the sterilization condition does not reach the standard, the sterilized Bacillus stearothermophilus spores are not completely killed, and the spores grow to produce acid in the culture medium after being inoculated into the culture medium containing the bromcresol purple indicator and incubated at 65 ℃, and the color of the pH indicator is changed into yellow. Conversely, if the sterilization conditions are met, spores will be killed completely, and the color of the pH indicator remains purple.
Traditionally, in the test method of antibiotic residue test and disinfection and sterilization effect evaluation, peptone and glucose are added into distilled water and stirred until completely dissolved, agar is additionally added for heating and dissolving if a solid culture medium needs to be prepared, the pH value is adjusted to 7.0-7.2 after dissolution, then 2% bromocresol purple ethanol solution is added, and after uniform mixing, high-pressure sterilization is carried out for 30-40 min at 115 ℃.
The bromocresol purple glucose peptone agar culture medium prepared by the method is suitable for growth and propagation of the bacillus stearothermophilus nutrient body, longer culture time is needed when bacillus stearothermophilus spore germination culture is carried out, and multiple experiments prove that the colony addition concentration is 5 × 105Under the condition of CFU, the time for changing the color of the culture medium needs 4-6 h.
After the culture medium is added with the culture auxiliary agent of the invention or the culture medium of the invention is adopted for thermophilic fat budWhen bacillus spores are germinated and cultivated, the concentration of the bacillus colonies is 5 × 105Under the condition of CFU, the time for changing the color of the culture medium is shortened to 2-3 h. The culture auxiliary agent can obviously shorten the germination time of the bacillus stearothermophilus spores, and further reduce the detection working time by using the bacillus stearothermophilus.
Example 1
Heating and uniformly mixing 2g of D L-tryptophan, 0.05g of glutathione, 0.02g of p-aminobenzoic acid and 4g of xanthan gum, adding 20g of fructose and 2g of beef powder, adding water to a constant volume of 100m L, stirring and dissolving to obtain a mixed solution, and filtering and sterilizing the mixed solution by using a 0.22-micrometer sterile filter to obtain the culture aid.
Adding 10.0g of peptone, 5.0g of glucose and 4.0g of agar into 800m L distilled water, heating and stirring until the peptone, the glucose and the agar are completely dissolved, adjusting the pH value to 7.1 +/-0.1, then adding 2% bromocresol purple ethanol solution 0.6m L, uniformly mixing, fixing the volume to 990m L, carrying out high-pressure sterilization at 115 ℃ for 30min, and naturally cooling to 60 ℃ to obtain the culture substrate.
Adding 10m of L culture auxiliary agent into the culture medium cooled to 60 ℃, and mixing uniformly to prepare the culture medium.
The Bacillus stearothermophilus spore suspension is added to the culture medium to a final spore concentration of 5 × 105CFU, adding culture medium containing spore suspension 0.2m L into a sterile 5m L centrifuge tube to obtain a detection culture tube, vertically placing, cooling to room temperature, and storing for later use.
Taking 10% sterilized skim milk powder solution as a negative sample, taking 10% skim milk powder solution containing penicillin G10 ug/L as a positive sample, respectively adding 0.1m L negative samples into a first group of three detection culture tubes, respectively adding 0.1m L positive samples into a second group of three detection culture tubes, placing the first group of detection culture tubes and the second group of detection culture tubes into a 56 ℃ constant temperature incubator for constant temperature culture, wherein the time for the negative samples to completely turn yellow is 2.5h, and the detection tubes of the positive samples are purple.
After the detection culture tube is stored for two weeks, the constant-temperature culture experiment is carried out, the time for the negative sample to turn yellow completely is 2.75h, and the detection tube for the positive sample is purple.
After the detection culture tube is stored for four weeks, the constant-temperature culture experiment is carried out, the time for the negative sample to turn yellow completely is 2.75h, and the detection tube for the positive sample is purple.
Comparative example 1
Adding 10.0g of peptone, 5.0g of glucose and 4.0g of agar into 800m L of distilled water, heating and stirring until the peptone, the glucose and the agar are completely dissolved, adjusting the pH value to 7.1 +/-0.1, then adding 2% bromocresol purple ethanol solution 0.6m L, uniformly mixing, fixing the volume to 1L, sterilizing at 115 ℃ under high pressure for 30min, naturally cooling to 60 ℃ to obtain the culture medium.
The Bacillus stearothermophilus spore suspension is added to the culture medium to a final spore concentration of 5 × 105CFU, adding culture medium containing spore suspension 0.2m L into a sterile 5m L centrifuge tube to obtain a detection culture tube, vertically placing, cooling to room temperature, and storing for later use.
The method comprises the steps of taking 10% sterilized skim milk powder as a negative sample, taking 10% skim milk powder containing penicillin G10 ug/L as a positive sample, adding 0.1m L negative samples into a first group of three detection culture tubes respectively, adding 0.1m L positive samples into a second group of three detection culture tubes respectively, placing the first group of detection culture tubes and the second group of detection culture tubes into a 56 ℃ constant temperature incubator for constant temperature culture, wherein the time for the negative sample to turn yellow completely is 4 hours, and the detection tubes of the positive sample are purple.
After the detection culture tube is stored for two weeks, the constant-temperature culture experiment is carried out, the time for the negative sample to turn yellow completely is 5h, and the detection tube for the positive sample is purple.
After the detection culture tube is stored for four weeks, the constant-temperature culture experiment is carried out, the time for the negative sample to turn yellow completely is 6h, and the detection tube for the positive sample is purple.
The experimental results of the comparative example 1 and the comparative example 1 show that the culture aid/culture medium of the present invention can effectively increase the germination and growth rate of bacillus stearothermophilus spores, ensure the storage stability of bacillus stearothermophilus spores in the culture medium, and greatly improve the application efficiency of spore industrialization.
Example 2
Heating and uniformly mixing 2g D L-tryptophan, 0.05g of glutathione, 0.02g of p-aminobenzoic acid and 4g of xanthan gum, adding 20g of fructose and 2g of beef powder, adding water to a constant volume of 100m L, stirring and dissolving to obtain a mixed solution, and filtering and sterilizing the mixed solution by using a 0.22um sterile filter to obtain the culture aid.
Adding 10.0g of peptone and 5.0g of glucose into 800m L distilled water, heating and stirring until the peptone and the glucose are completely dissolved, adjusting the pH value to 7.1 +/-0.1, then adding 2% bromocresol purple ethanol solution 0.6m L, uniformly mixing, fixing the volume to 990m L, sterilizing at 115 ℃ under high pressure for 30min, and naturally cooling to 60 ℃ to obtain the culture substrate.
Adding 10m L culture auxiliary agent into the culture medium cooled to 60 ℃, mixing uniformly to obtain a culture medium, and subpackaging the culture medium with sterilized centrifuge tubes according to 5m L/tube to obtain a detection culture tube for later use.
Bacillus stearothermophilus spores were added to the detection culture tube to a final concentration of 1CFU, calculated on 10m L medium, as a positive sample.
The bacteria content is 5 × 105And (3) autoclaving the CFU bacillus stearothermophilus sheets for 20min at the temperature of 121 ℃ to prepare sterilized sheets, and placing the sterilized sheets in a culture detection tube to be used as samples to be detected.
And placing the positive sample and the sample to be detected in a constant temperature incubator at 56 ℃ for constant temperature culture.
The yellowing time of the positive sample is 24h, and the sample to be detected containing the sterilization sheet is purple.
Comparative example 2
Adding 10.0g of peptone and 5.0g of glucose into 800m L of distilled water, heating and stirring until the peptone and the glucose are completely dissolved, adjusting the pH value to 7.1 +/-0.1, then adding 2% bromocresol purple ethanol solution 0.6m L, uniformly mixing, fixing the volume to 1L, carrying out high-pressure sterilization at 115 ℃ for 30min for standby, naturally cooling, and packaging with a sterilized centrifuge tube according to 5m L/tube to obtain a detection culture tube for standby.
Bacillus stearothermophilus spores were added to the detection culture tube to a final concentration of 1CFU, calculated on 10m L medium, as a positive sample.
The bacteria content is 5 × 105Sterilizing CFU Bacillus stearothermophilus spore tablet at 121 deg.C for 20min to obtain sterilized tablet, and sterilizingThe sterilization piece is arranged in the culture detection tube and is used as a sample to be detected.
And placing the positive sample and the sample to be detected in a constant temperature incubator at 56 ℃ for constant temperature culture.
The yellowing time of the positive sample is 48h, and the sample to be detected containing the sterilization sheet is purple.
The experimental results of comparative example 2 and comparative example 2 show that the culture aid/culture medium of the present invention can increase the germination and growth rate of bacillus stearothermophilus spores, and can effectively increase the germination and growth rate of bacillus stearothermophilus spores even when there are few bacillus stearothermophilus spores, thereby improving the application efficiency of spore industrialization.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (6)
1. The bacillus stearothermophilus spore culture auxiliary is characterized by comprising 1-20 g of fructose, 0.1-10 g of D L-tryptophan, 0.01-5 g of glutathione, 0.1-10 mg of p-aminobenzoic acid, 0.1-10 g of xanthan gum and 0.1-10 g of beef powder per 100m L, and the culture auxiliary is added into a culture medium for culturing bacillus stearothermophilus to culture the bacillus stearothermophilus.
2. The culture aid according to claim 1, wherein the culture aid is filtered through a sterile filter.
3. A Bacillus stearothermophilus spore culture medium, which comprises a culture medium and the culture auxiliary of any one of claims 1 to 2, wherein 5-20 g of peptone, 1-20 g of glucose and 0.3-1 m L of bromocresol purple ethanol solution are contained in every 1000m L of the culture medium.
4. The culture medium according to claim 3, wherein the culture auxiliary is 1-20 m L per 1000m L of the culture medium.
5. The culture medium according to claim 3, wherein the agar is contained in an amount of 3.0 to 20g per 1000m L of the culture medium.
6. The use of the Bacillus stearothermophilus spore culture medium according to any one of claims 3 to 5, wherein the culture medium is used for antibiotic residual test or for the evaluation of disinfection and sterilization effect.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1778892A (en) * | 2004-11-19 | 2006-05-31 | 上海爱普香料有限公司 | Parvobacteria of high-yield 3-hydroxy-butanoic butanone |
| CN102424830A (en) * | 2011-12-15 | 2012-04-25 | 江南大学 | Method for enhancing 2-keto-L-gulonic acid (2-KLG) production intensity of Ketogulonigenium vulgare by adding reduced glutathione (GSH) |
| CN105638651A (en) * | 2015-12-19 | 2016-06-08 | 佛山市艳晖生物科技有限公司 | Preparation method of Brevibacillus atrophaeus wettable powder |
| CN106867940A (en) * | 2017-03-16 | 2017-06-20 | 青岛农业大学 | A kind of method for promoting the growth of bacillus stearothermophilus gemma to sprout |
-
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1778892A (en) * | 2004-11-19 | 2006-05-31 | 上海爱普香料有限公司 | Parvobacteria of high-yield 3-hydroxy-butanoic butanone |
| CN102424830A (en) * | 2011-12-15 | 2012-04-25 | 江南大学 | Method for enhancing 2-keto-L-gulonic acid (2-KLG) production intensity of Ketogulonigenium vulgare by adding reduced glutathione (GSH) |
| CN105638651A (en) * | 2015-12-19 | 2016-06-08 | 佛山市艳晖生物科技有限公司 | Preparation method of Brevibacillus atrophaeus wettable powder |
| CN106867940A (en) * | 2017-03-16 | 2017-06-20 | 青岛农业大学 | A kind of method for promoting the growth of bacillus stearothermophilus gemma to sprout |
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