CN107469148A - A kind of new type bone repair of cartilage support and preparation method thereof - Google Patents

A kind of new type bone repair of cartilage support and preparation method thereof Download PDF

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Publication number
CN107469148A
CN107469148A CN201710631476.9A CN201710631476A CN107469148A CN 107469148 A CN107469148 A CN 107469148A CN 201710631476 A CN201710631476 A CN 201710631476A CN 107469148 A CN107469148 A CN 107469148A
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layer
cartilage
repair
bone
prepared
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韦加娜
卢亢
吴帅
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Guangdong Bao Bao Medical Equipment Technology Research Institute Co Ltd
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Guangdong Bao Bao Medical Equipment Technology Research Institute Co Ltd
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    • A61L27/58—Materials at least partially resorbable by the body
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B33—ADDITIVE MANUFACTURING TECHNOLOGY
    • B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
    • B33Y10/00—Processes of additive manufacturing
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
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    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/06—Materials or treatment for tissue regeneration for cartilage reconstruction, e.g. meniscus

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Abstract

The invention discloses a kind of new type bone repair of cartilage support and preparation method thereof, belong to two layers of repair layer composition of osseous tissue renovating material preparing technical field, upper strata is repair of cartilage layer, lower floor is Bone Defect Repari layer, upper and lower two layers of repair layer is prepared by 3D printing technique, the repair of cartilage layer is prepared by two kinds of materials of alginates and chitosan, and wherein the mass ratio of alginates and chitosan is 2:1~1:2, there is the dual-use function for repairing bone and cartilage, and double-deck repair layer is respectively provided with anti-microbial property, can prevent from repairing the infection at position.

Description

A kind of new type bone-repair of cartilage support and preparation method thereof
Technical field：
It is public the invention belongs to the technical field of biological materials of organizational project, particularly osseous tissue renovating material preparing technical field A kind of new type bone-repair of cartilage support and preparation method thereof is opened.
Background technology：
The reasons such as wound, inflammation cause articular cartilage damage or defect is case common in clinic.But adult articular cartilage Repair ability it is excessively poor, if cannot treat, damage usually develops into degenerative arthritis and even results in whole function of joint Damage.The damage for damaging often concurrent subchondral bone of articular cartilage simultaneously, and the treatment of bone-cartilage damage at present has one Fixed difficulty.With organizational project principle, structure bone-cartilage integration organization engineering rack can efficiently solve this problem.
The content of the invention
The present invention proposes a kind of new type bone-repair of cartilage support and its preparation side for shortcoming present in prior art Method, by organizational project principle, bone-cartilage recovery support is built using 3D printing technique, have and repair the dual of bone and cartilage Function, and double-deck repair layer is respectively provided with anti-microbial property, can prevent from repairing the infection at position.
To achieve the above object, the technical scheme is that being made up of two layers of repair layer, upper strata is repair of cartilage layer, under Layer is Bone Defect Repari layer, and upper and lower two layers of repair layer is prepared by 3D printing technique.
Preferably, repair of cartilage layer and Bone Defect Repari layer are cylindrical three-dimensional pore space structure, and hole is 100 ~ 300 μm of the length of side Circular port, a diameter of 2 ~ 3cm of recovery support, the height of repair of cartilage layer and Bone Defect Repari layer is 1 ~ 2cm.
Preferably, the repair of cartilage layer is prepared by two kinds of materials of alginates and chitosan, wherein alginates and shell The mass ratio of glycan is 2:1~1:2.
Preferably, the Bone Defect Repari layer is prepared by alginates and bioactivity glass, and wherein alginates are lived with biology Property glass mass ratio be 5:1~3:1.
Preferably, bioactivity glass is borate biological activity glass, and its particle diameter is 500nm ~ 1 μm.
To achieve the above object, another technical scheme of the invention is：A kind of preparation side of new type bone-repair of cartilage support Method, it is characterised in that comprise the following steps：
S1：Prepare Alginate hydrogel：Alginic acid salt powder is dissolved in distilled water, 0.01 ~ 0.02g/ml alginic acid is prepared Salt gel；
S2：Prepare aquagel：Chitosan powder is dissolved in volumetric concentration as in 1% dilute acetic acid solution, stirring is prepared into To 0.02 ~ 0.03g/ml chitosan gel rubbers；
S3：Prepare alginates-chitosan mixed gel：Alginate jelly made from S1 and S2 is blended in one with chitosan gel rubber Rise, stir, it is standby；
S4：Prepare alginates-bioactivity glass mixed gel：Alginic acid salt powder is dissolved in distilled water, is prepared 0.01 ~ 0.02g/ml Alginate hydrogels, then bioactivity glass is added into Alginate hydrogel, it is uniformly mixing to obtain alginic acid Salt-bioactivity glass mixing hydrogel, it is standby；
S5：Prepare bone-cartilage recovery support：The alginates prepared-bioactivity glass mixed gel is added into 3D printer Charging spout in, set the program of 3D printer, start 3D printer, be prepared thickness be 1 ~ 2cm, the length of side be 100 ~ 300 μ The Bone Defect Repari layer of m circular ports, it is after 1.03 × 10-2g/ml calcium chloride solutions produce solidification that concentration, which is slowly added dropwise, to Bone Defect Repari layer Bone Defect Repari layer；Alginates-chitosan mixed gel is added into the charging spout of 3D printer again, sets the journey of 3D printer Sequence, start 3D printer, be prepared above Bone Defect Repari layer thickness be 1 ~ 2cm, the length of side be the soft of 100 ~ 300 μm of circular ports Bone Defect Repari layer, concentration is slowly added dropwise to middle repair of cartilage layer and is repaiied for the cartilage after 1.03 × 10-2g/ml calcium chloride solutions solidify Cladding；Excessive calcium chloride solution is removed, produces bone-cartilage recovery support.
Beneficial effects of the present invention are：
Method is simple, and preparation time is short, and required raw material sources are enriched, cheap, and production cost is low, and bone-cartilage is repaiied Multiple support is made up of repair of cartilage layer and Bone Defect Repari layer, simulates human body bone and cartilage structure completely；
Repair of cartilage layer, by both mass ratioes of regulation, can make cartilage because alginates are different from degradation of chitosan speed The degradation rate of repair layer preferably meets cartilage and grows into speed；
Bone Defect Repari layer can be such that the mechanical strength of Bone Defect Repari layer preferably connects by adjusting the addition of bioactivity glass The mechanical strength of person of modern times's body bone；
The chitosan of repair of cartilage layer and the borate biological activity glass of Bone Defect Repari layer are respectively provided with good antibiotic property Can, it can be very good to prevent damage location infection from occurring；
Borate biological activity glass in Bone Defect Repari layer has bioactivity height, has good key with bone and cartilage Close, a series of surface reactions can occur rapidly in implant site, ultimately form the carbonate with bone and soft tissue good result Hydroxyapatite layer.
Embodiment
Technical scheme is described further with reference to embodiment.
Embodiment 1
A kind of new type bone-repair of cartilage support and preparation method thereof is provided, alginic acid salt powder is dissolved in distilled water, is prepared into To 0.015g/ml alginate jellies；Chitosan powder is dissolved in 1%（v/v）In dilute acetic acid solution, stirring is prepared 0.02g/ml chitosan gel rubbers；Alginate jelly made from above-mentioned steps and chitosan gel rubber are stirred produce alginates- Chitosan mixing hydrogel, standby, alginates and chitosan mass ratio in the alginates-chitosan mixing hydrogel are 1: 1；Alginic acid salt powder is dissolved in distilled water, 0.015g/ml alginate jellies are prepared, then add into alginate jelly Bioactivity glass, alginates-bioactivity glass mixing hydrogel is uniformly mixing to obtain, standby, the alginates-biology is living Property glass mixing hydrogel in alginates and bioactivity glass quality be 4:1；Alginates-the bioactivity glass that will be prepared Mix hydrogel to add in the charging spout of 3D printer, set the program of 3D printer, start 3D printer, thickness is prepared For the Bone Defect Repari layer that 1.5cm, the length of side are 100 ~ 300 μm of circular ports, concentration is slowly added dropwise as 1.03 × 10 to Bone Defect Repari layer-2g/ml Calcium chloride solution produces the Bone Defect Repari layer after solidification；Alginates-chitosan mixing water is added into the charging spout of 3D printer again Gel, the program of 3D printer is set, start 3D printer, thickness is prepared above Bone Defect Repari layer as 1.5cm, the length of side For the repair of cartilage layer of 100 ~ 300 μm of circular ports, concentration is slowly added dropwise as 1.03 × 10 to middle repair of cartilage layer-2G/ml calcium chloride Solution is the repair of cartilage layer after solidifying；Excessive calcium chloride solution is removed, produces bone-cartilage recovery support.
Embodiment 2
A kind of new type bone-repair of cartilage support and preparation method thereof is provided, alginic acid salt powder is dissolved in distilled water, is prepared into To 0.015g/ml alginate jellies；Chitosan powder is dissolved in 1%（v/v）In dilute acetic acid solution, stirring is prepared 0.02g/ml chitosan gel rubbers；Alginate jelly made from above-mentioned steps and chitosan gel rubber are stirred produce alginates- Chitosan mixing hydrogel, standby, alginates and chitosan mass ratio in the alginates-chitosan mixing hydrogel are 2: 1；Alginic acid salt powder is dissolved in distilled water, 0.015g/ml alginate jellies are prepared, then add into alginate jelly Bioactivity glass, alginates-bioactivity glass mixing hydrogel is uniformly mixing to obtain, standby, the alginates-biology is living Property glass mixing hydrogel in alginates and bioactivity glass quality be 4:1；Alginates-the bioactivity glass that will be prepared Mix hydrogel to add in the charging spout of 3D printer, set the program of 3D printer, start 3D printer, thickness is prepared For the Bone Defect Repari layer that 1.5cm, the length of side are 100 ~ 300 μm of circular ports, concentration is slowly added dropwise as 1.03 × 10 to Bone Defect Repari layer-2g/ml Calcium chloride solution produces the Bone Defect Repari layer after solidification；Alginates-chitosan mixing water is added into the charging spout of 3D printer again Gel, the program of 3D printer is set, start 3D printer, thickness is prepared above Bone Defect Repari layer as 1.5cm, the length of side For the repair of cartilage layer of 100 ~ 300 μm of circular ports, concentration is slowly added dropwise as 1.03 × 10 to middle repair of cartilage layer-2G/ml calcium chloride Solution is the repair of cartilage layer after solidifying；Excessive calcium chloride solution is removed, produces bone-cartilage recovery support.
Embodiment 3
A kind of new type bone-repair of cartilage support and preparation method thereof is provided, alginic acid salt powder is dissolved in distilled water, is prepared into To 0.015g/ml alginate jellies；Chitosan powder is dissolved in 1%（v/v）In dilute acetic acid solution, stirring is prepared 0.02g/ml chitosan gel rubbers；Alginate jelly made from above-mentioned steps and chitosan gel rubber are stirred produce alginates- Chitosan mixing hydrogel, standby, alginates and chitosan mass ratio in the alginates-chitosan mixing hydrogel are 1: 2；Alginic acid salt powder is dissolved in distilled water, 0.015g/ml alginate jellies are prepared, then add into alginate jelly Bioactivity glass, alginates-bioactivity glass mixing hydrogel is uniformly mixing to obtain, standby, the alginates-biology is living Property glass mixing hydrogel in alginates and bioactivity glass quality be 4:1；Alginates-the bioactivity glass that will be prepared Mix hydrogel to add in the charging spout of 3D printer, set the program of 3D printer, start 3D printer, thickness is prepared For the Bone Defect Repari layer that 1.5cm, the length of side are 100 ~ 300 μm of circular ports, concentration is slowly added dropwise as 1.03 × 10 to Bone Defect Repari layer-2g/ml Calcium chloride solution produces the Bone Defect Repari layer after solidification；Alginates-chitosan mixing water is added into the charging spout of 3D printer again Gel, the program of 3D printer is set, start 3D printer, thickness is prepared above Bone Defect Repari layer as 1.5cm, the length of side For the repair of cartilage layer of 100 ~ 300 μm of circular ports, concentration is slowly added dropwise as 1.03 × 10 to middle repair of cartilage layer-2G/ml calcium chloride Solution is the repair of cartilage layer after solidifying；Excessive calcium chloride solution is removed, produces bone-cartilage recovery support.
Embodiment 4
A kind of new type bone-repair of cartilage support and preparation method thereof is provided, alginic acid salt powder is dissolved in distilled water, is prepared into To 0.015g/ml alginate jellies；Chitosan powder is dissolved in 1%（v/v）In dilute acetic acid solution, stirring is prepared 0.02g/ml chitosan gel rubbers；Alginate jelly made from above-mentioned steps and chitosan gel rubber are stirred produce alginates- Chitosan mixing hydrogel, standby, alginates and chitosan mass ratio in the alginates-chitosan mixing hydrogel are 1: 1；Alginic acid salt powder is dissolved in distilled water, 0.015g/ml alginate jellies are prepared, then add into alginate jelly Bioactivity glass, alginates-bioactivity glass mixing hydrogel is uniformly mixing to obtain, standby, the alginates-biology is living Property glass mixing hydrogel in alginates and bioactivity glass quality be 5:1；Alginates-the bioactivity glass that will be prepared Mix hydrogel to add in the charging spout of 3D printer, set the program of 3D printer, start 3D printer, thickness is prepared For the Bone Defect Repari layer that 1.5cm, the length of side are 100 ~ 300 μm of circular ports, concentration is slowly added dropwise as 1.03 × 10 to Bone Defect Repari layer-2g/ml Calcium chloride solution produces the Bone Defect Repari layer after solidification；Alginates-chitosan mixing water is added into the charging spout of 3D printer again Gel, the program of 3D printer is set, start 3D printer, thickness is prepared above Bone Defect Repari layer as 1.5cm, the length of side For the repair of cartilage layer of 100 ~ 300 μm of circular ports, concentration is slowly added dropwise as 1.03 × 10 to middle repair of cartilage layer-2G/ml calcium chloride Solution is the repair of cartilage layer after solidifying；Excessive calcium chloride solution is removed, produces bone-cartilage recovery support.
Embodiment 5
A kind of new type bone-repair of cartilage support and preparation method thereof is provided, alginic acid salt powder is dissolved in distilled water, is prepared into To 0.015g/ml alginate jellies；Chitosan powder is dissolved in 1%（v/v）In dilute acetic acid solution, stirring is prepared 0.02g/ml chitosan gel rubbers；Alginate jelly made from above-mentioned steps and chitosan gel rubber are stirred produce alginates- Chitosan mixing hydrogel, standby, alginates and chitosan mass ratio in the alginates-chitosan mixing hydrogel are 1: 1；Alginic acid salt powder is dissolved in distilled water, 0.015g/ml alginate jellies are prepared, then add into alginate jelly Bioactivity glass, alginates-bioactivity glass mixing hydrogel is uniformly mixing to obtain, standby, the alginates-biology is living Property glass mixing hydrogel in alginates and bioactivity glass quality be 3:1；Alginates-the bioactivity glass that will be prepared Mix hydrogel to add in the charging spout of 3D printer, set the program of 3D printer, start 3D printer, thickness is prepared For the Bone Defect Repari layer that 1.5cm, the length of side are 100 ~ 300 μm of circular ports, concentration is slowly added dropwise as 1.03 × 10 to Bone Defect Repari layer-2g/ml Calcium chloride solution produces the Bone Defect Repari layer after solidification；Alginates-chitosan mixing water is added into the charging spout of 3D printer again Gel, the program of 3D printer is set, start 3D printer, thickness is prepared above Bone Defect Repari layer as 1.5cm, the length of side For the repair of cartilage layer of 100 ~ 300 μm of circular ports, concentration is slowly added dropwise as 1.03 × 10 to middle repair of cartilage layer-2G/ml calcium chloride Solution is the repair of cartilage layer after solidifying；Excessive calcium chloride solution is removed, produces bone-cartilage recovery support.
Embodiment 6
Comparative example：Using a kind of method for preparing tissue engineered bone cartilage frame（Application No. 201010107478.6）Prepare and Into bone cartilage frame.
Embodiment 7
A kind of new type bone-repair of cartilage support that above-described embodiment 1 ~ 6 is prepared, Mechanics Performance Testing is carried out, utilizes ten thousand Energy testing machine determines its compressive strength, and result of the test is as follows：
Test group Embodiment 1 Embodiment 2 Embodiment 3 Embodiment 4 Embodiment 5 Embodiment 6
Compressive strength 20.7MPa 28.3MPa 16.5MPa 32.2MPa 13.9MPa 25.7MP
It can be learnt from result data, embodiment 6 is made up of animal cancellous bone completely, though the present invention takes gel-like as raw material, But there is the mechanical property suitable with the bone-cartilage recovery support formed using animal cancellous bone as raw material, solve existing The problem of in technology using bone-cartilage recovery support poor mechanical property of the Native Gel class as the formation of raw material.
Embodiment 8
New zealand white rabbit osteochondral defect model reparative experiment
The foundation of animal model：Take new zealand white rabbit 40,3 months age sizes, body weight 2kg or so, operation consent 6h fasting Prohibit drink.4 groups are randomly divided into, every group of 10 knees, divides A, B, C, D group, with 2.5% yellow Jackets auricular vein injecting anesthetic.Anesthesia After appropriate, its four limbs is properly fixed, kneecap ligament lateral border, fully exposure distal part of femur external condyle heavy burden area are cut successively, at it On do a diameter 4mm, depth 3mm cylindrical defect, drill subchondral bone, after removing the blood clot in defect, ABC components The bone-cartilage recovery support of the formation of embodiment 2,4,6 is not inserted, and D groups do blank control and are not implanted into any material.Layer-by-layer suture is hindered Mouthful, postoperative iodophor disinfection wound, outer fixation, the dual anti-prevention infection of intramuscular injection mycillin are not had to after gauze wrapping.Postoperative single cage is fed Support, each group carries out CT examination respectively within 8,12,16 weeks, observes osseocartilaginous reparation situation.Observe and identify through CT, it is found that ABC is impartial Good reparation, and the ratio that AB groups are substantially recovered is very fast, is embodied at defect and is filled by cambium, repair surface is put down It is whole, though and C groups have at cambium filling defect, than smooth, color and luster is dim on surface.And the bone cartilage of defect is only in D groups Less reparation is only obtained, the repairing effect for the bone repair of cartilage support for as a result proving to take the present invention to be prepared will be substantially Better than the bone repair of cartilage support for using animal cancellous bone to be prepared for raw material.
Obviously, the above embodiment of the present invention is only intended to clearly illustrate example of the present invention, and is not pair The restriction of embodiments of the present invention；For those of ordinary skill in the field, may be used also on the basis of the above description To make other changes in different forms, there is no necessity and possibility to exhaust all the enbodiments；It is all this All any modification, equivalent and improvement made within the spirit and principle of invention etc., should be included in the claims in the present invention Protection domain within.

Claims (6)

  1. A kind of 1. new type bone-repair of cartilage support, it is characterised in that it is made up of two layers of repair layer, upper strata is repair of cartilage layer, under Layer is Bone Defect Repari layer, and upper and lower two layers of repair layer is prepared by 3D printing technique.
  2. 2. a kind of new type bone-repair of cartilage support according to claim 1, it is characterised in that repair of cartilage layer is repaiied with bone Cladding be cylinder three-dimensional pore space structure, hole be 100 ~ 300 μm of the length of side circular port, a diameter of 2 ~ 3cm of recovery support, The height of repair of cartilage layer and Bone Defect Repari layer is 1 ~ 2cm.
  3. A kind of 3. new type bone-repair of cartilage support according to claim 1, it is characterised in that the repair of cartilage layer by Two kinds of materials of alginates and chitosan are prepared, and wherein the mass ratio of alginates and chitosan is 2:1~1:2.
  4. 4. a kind of new type bone-repair of cartilage support according to claim 1, it is characterised in that the Bone Defect Repari layer is by algae Hydrochlorate and bioactivity glass are prepared, and wherein the mass ratio of alginates and bioactivity glass is 5:1~3:1.
  5. 5. a kind of new type bone-repair of cartilage support according to claim 4, it is characterised in that bioactivity glass is boron Hydrochlorate bioactivity glass, its particle diameter are 500nm ~ 1 μm.
  6. A kind of a kind of 6. preparation method of new type bone-repair of cartilage support as described in claim 1 ~ 5, it is characterised in that bag Include following steps：
    S1：Prepare Alginate hydrogel：Alginic acid salt powder is dissolved in distilled water, 0.01 ~ 0.02g/ml alginic acid is prepared Salt gel；
    S2：Prepare aquagel：Chitosan powder is dissolved in volumetric concentration as in 1% dilute acetic acid solution, stirring is prepared into To 0.02 ~ 0.03g/ml chitosan gel rubbers；
    S3：Prepare alginates-chitosan mixed gel：Alginate jelly made from S1 and S2 is blended in one with chitosan gel rubber Rise, stir, it is standby；
    S4：Prepare alginates-bioactivity glass mixed gel：Alginic acid salt powder is dissolved in distilled water, is prepared 0.01 ~ 0.02g/ml Alginate hydrogels, then bioactivity glass is added into Alginate hydrogel, it is uniformly mixing to obtain alginic acid Salt-bioactivity glass mixing hydrogel, it is standby；
    S5：Prepare bone-cartilage recovery support：The alginates prepared-bioactivity glass mixed gel is added into 3D printer Charging spout in, set the program of 3D printer, start 3D printer, be prepared thickness be 1 ~ 2cm, the length of side be 100 ~ 300 μ The Bone Defect Repari layer of m circular ports, it is after 1.03 × 10-2g/ml calcium chloride solutions produce solidification that concentration, which is slowly added dropwise, to Bone Defect Repari layer Bone Defect Repari layer；Alginates-chitosan mixed gel is added into the charging spout of 3D printer again, sets the journey of 3D printer Sequence, start 3D printer, be prepared above Bone Defect Repari layer thickness be 1 ~ 2cm, the length of side be the soft of 100 ~ 300 μm of circular ports Bone Defect Repari layer, concentration is slowly added dropwise to middle repair of cartilage layer and is repaiied for the cartilage after 1.03 × 10-2g/ml calcium chloride solutions solidify Cladding；Excessive calcium chloride solution is removed, produces bone-cartilage recovery support.
CN201710631476.9A 2017-07-28 2017-07-28 A kind of new type bone repair of cartilage support and preparation method thereof Pending CN107469148A (en)

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CN108992212A (en) * 2018-08-03 2018-12-14 广州迈普再生医学科技股份有限公司 Bone-cartilage one recovery support and preparation method thereof
CN109223255A (en) * 2018-09-27 2019-01-18 叶川 A kind of biogel material, artificial knee joint and preparation method thereof
CN109938885A (en) * 2019-04-21 2019-06-28 西北工业大学 A method of bone cartilage integrated bracket is prepared based on 3DP and laser melting coating combination process
CN110665057A (en) * 2019-11-07 2020-01-10 上海健康医学院 Preparation method of hydroxyapatite/PLGA double-layer stent
CN113478815A (en) * 2021-06-30 2021-10-08 杭州电子科技大学 3D printing method of osteochondral scaffold with highly bionic mechanical property

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CN101219069A (en) * 2008-01-25 2008-07-16 中国人民武装警察部队医学院附属医院 Double-layer composite bracket for renovating cartilage
CN105770988A (en) * 2016-04-20 2016-07-20 清华大学深圳研究生院 Bone repairing biological ceramic material based on 3D printing and preparation method thereof

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CN101219069A (en) * 2008-01-25 2008-07-16 中国人民武装警察部队医学院附属医院 Double-layer composite bracket for renovating cartilage
CN105770988A (en) * 2016-04-20 2016-07-20 清华大学深圳研究生院 Bone repairing biological ceramic material based on 3D printing and preparation method thereof

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108992212A (en) * 2018-08-03 2018-12-14 广州迈普再生医学科技股份有限公司 Bone-cartilage one recovery support and preparation method thereof
CN109223255A (en) * 2018-09-27 2019-01-18 叶川 A kind of biogel material, artificial knee joint and preparation method thereof
CN109938885A (en) * 2019-04-21 2019-06-28 西北工业大学 A method of bone cartilage integrated bracket is prepared based on 3DP and laser melting coating combination process
CN110665057A (en) * 2019-11-07 2020-01-10 上海健康医学院 Preparation method of hydroxyapatite/PLGA double-layer stent
CN110665057B (en) * 2019-11-07 2021-10-01 上海健康医学院 Preparation method of hydroxyapatite/PLGA double-layer stent
CN113478815A (en) * 2021-06-30 2021-10-08 杭州电子科技大学 3D printing method of osteochondral scaffold with highly bionic mechanical property

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