CN107469094A - A kind of nano material for being used for magnetic resonance imaging and/or photo-thermal therapy and preparation method thereof - Google Patents

A kind of nano material for being used for magnetic resonance imaging and/or photo-thermal therapy and preparation method thereof Download PDF

Info

Publication number
CN107469094A
CN107469094A CN201710812141.7A CN201710812141A CN107469094A CN 107469094 A CN107469094 A CN 107469094A CN 201710812141 A CN201710812141 A CN 201710812141A CN 107469094 A CN107469094 A CN 107469094A
Authority
CN
China
Prior art keywords
nano material
preparation
dopa
amine
photo
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201710812141.7A
Other languages
Chinese (zh)
Other versions
CN107469094B (en
Inventor
唐建斌
夏明春
申有青
刘祥瑞
周珠贤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zhejiang University ZJU
Original Assignee
Zhejiang University ZJU
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zhejiang University ZJU filed Critical Zhejiang University ZJU
Priority to CN201710812141.7A priority Critical patent/CN107469094B/en
Publication of CN107469094A publication Critical patent/CN107469094A/en
Application granted granted Critical
Publication of CN107469094B publication Critical patent/CN107469094B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/06—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations
    • A61K49/08—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by the carrier
    • A61K49/10—Organic compounds
    • A61K49/12—Macromolecular compounds
    • A61K49/126—Linear polymers, e.g. dextran, inulin, PEG
    • A61K49/128—Linear polymers, e.g. dextran, inulin, PEG comprising multiple complex or complex-forming groups, being either part of the linear polymeric backbone or being pending groups covalently linked to the linear polymeric backbone
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K41/00—Medicinal preparations obtained by treating materials with wave energy or particle radiation ; Therapies using these preparations
    • A61K41/0052—Thermotherapy; Hyperthermia; Magnetic induction; Induction heating therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/06—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations
    • A61K49/08—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by the carrier
    • A61K49/085—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by the carrier conjugated systems

Landscapes

  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Epidemiology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Radiology & Medical Imaging (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The invention discloses a kind of for nano material of magnetic resonance imaging and/or photo-thermal therapy and preparation method thereof, belong to the photo-thermal therapy diagnosis and treatment integration field that magnetic resonance imaging mediates.The preparation method of the nano material, including：In the presence of copper ion, in aqueous medium under the amine-modified Gd coordination compound of DOPA and the amine-modified polyethylene glycol heating condition of DOPA, the partial oxidation Viability higher semiquinone or quinone of catechol, product is spontaneous to agglomerate into described nano material.Small molecule gadolinium contrast agent is transformed into macromolecular gadolinium contrast agent by the present invention by oxidative coupling reaction, has the advantages that relaxivity is high, and circulation time in vivo is long, targeting and toxic side effect are small；Nano material produced by the present invention has high light thermal transition efficiency near infrared region, it can be applied to as photo-thermal therapy agent in photo-thermal therapy, the enrichment condition of the position, size and light treatment agent of tumour in tumor tissues can be determined by mr techniques simultaneously, for evaluating therapeutic effect.

Description

It is a kind of to be used for magnetic resonance imaging and/or nano material and its preparation of photo-thermal therapy Method
Technical field
The present invention relates to the photo-thermal therapy diagnosis and treatment integration field of magnetic resonance imaging mediation, and in particular to one kind is total to for magnetic Shake nano material and preparation method thereof of imaging and/or photo-thermal therapy.
Background technology
Photo-thermal therapy (PTT) is to utilize the material with high light thermal conversion efficiency under the conditions of near infrared light photograph by luminous energy Heat energy is converted into, causes tumour cell localized hyperthermia, so as to kill a kind for the treatment of method of cancer cell.Asked at past several years, The nano material that many kinds have strong near infrared absorption includes various gold nano grain, carbon nanomaterial, palladium piece, copper sulfide Nano particle even also has some organic nanometer granules to be all used for tumor thermal therapy as photothermal reagent.Due to biological tissue The absorption of interior water and protein near infrared light is weaker, so near infrared light is best to the penetrability of tissue, preferable photo-thermal is controlled Stronger absorption should be had near infrared light region (650-950nm) by treating agent.
Ensure that effectively exposure is under light illumination the necessary condition of photo-thermal therapy to whole tumour, but only rely on photo-thermal therapy PTT can be restricted because knub position and detection therapeutic effect can not be determined.Imaging technique can make up this defect, There is provided the available information such as the position about tumour, shape, size, while can determine light treatment agent swollen by imaging technique The enrichment condition of tumor tissue, evaluate therapeutic effect.So it is very must that photo-thermal therapy and imaging technique, which are organically combined, Want.
Magnetic resonance imaging (MRI) is a kind of difference with organism different tissues moisture ratio, and in water residing for proton The image technology being imaged based on the difference of chemical environment.Compare other image technologies, as area of computer aided X-ray into As technology (CT), Positron Emission Computed Tomography art (PET), ultrasonic imaging (USG), optical imagery (OI), magnetic resonance Imaging has the advantages that high-resolution and high sensitivity and causes extensive pass due to no radioactive ionization infringement Note.Although MRI spatial resolution is very high, the simple sensitivity using MRI imagings is not high, thus needs to use contrast agent (MRI contrast agent) comes associated image, enhancing signal contrast and the resolution ratio for improving soft-tissue image.
Magnetic resonance imaging contrast is generally the complex of paramagnetic metal ion, and its part is mostly to contain polyamino and more carboxylics The linear and cyclic compound of base.Clinical conventional contrast agent such as DTPA-Gd (Gd-DTPA) at present, it is a kind of small Molecular ion type contrast agent, because osmotic pressure is higher in vivo, excluded rapidly after The book of Changes renal metabolism, in vivo RT compared with It is short, selectivity and targeting without tissue and organ.Further, Gd-DTPA is bonded in biodegradable macromolecular On carrier, macromolecular contrast agent is formed, the low molecular speed of rotation can be dropped, improves relaxation rate, and can be relatively long in the blood vessel Stable concentration is kept in time, is advantageous to angiogram, also known as blood pool contrast agent.
Therefore, exploitation is a kind of not only may be used as magnetic resonance imaging contrast but also may be used as the novel-section of photo-thermal therapy agent Material, that is, meet the purpose of above-mentioned imaging and photo-thermal therapy diagnosis and treatment integration, this is that those skilled in the art need what is solved to ask Topic.
The content of the invention
Object of the present invention is to provide a kind of new nano material, the nano material have higher relaxation rate and There is high light thermal transition efficiency under the conditions of near infrared light photograph, magnetic resonance imaging and photo-thermal therapy can be applied to, with Realize the diagnosis and treatment integration of tumour.
To achieve the above object, the present invention adopts the following technical scheme that：
A kind of preparation method for the nano material for being used for magnetic resonance imaging and/or photo-thermal therapy, including：In the work of copper ion Under, in aqueous medium the amine-modified Gd coordination compound of DOPA and the amine-modified polyethylene glycol of DOPA in a heated condition, catechol Partial oxidation Viability higher semiquinone or quinone, product is spontaneous to agglomerate into described nano material.
The Gd coordination compound and polyethylene glycol that dopamine is modified with the preparation method of the present invention are acted on and heated in copper ion Under the conditions of oxidative coupling reaction occurs, be specially：Phenolic hydroxyl group dehydrogenation on two kinds of raw material dopamines, mutually roll into a ball between its product Combinate form is into granular nanometer level material.
Preferably, shown in the structural formula such as formula (I) of the amine-modified Gd coordination compound of the DOPA：
Above-mentioned complex DTPA (HTA)2@Gd preparation can refer to document (Parac-Vogt, et al.Heterobimetallic gadolinium(III)-iron(III)complex of DTPA-bis(3- hydroxytyramide).Journal of Alloys and Compounds 374(2004):325-329.)。
Preferably, the amine-modified polyethylene glycol of the DOPA is methoxyl group-poly- second two that molecular weight is 2000-4000Da Alcohol-dopamine.Its preparation can refer to document (Qiaochu Li, et al.Controlling Hydrogel Mechanics via Bio-Inspired Polymer-Nanoparticle Bond Dynamics.ACS Nano,2016,10(1),1317- 1324.).Shown in its structural formula such as following formula (II)：
More preferably, the methoxypolyethylene glycol that it is 2000Da that the amine-modified polyethylene glycol of the DOPA, which is molecular weight ,-more Bar amine.
Preferably, the molar ratio of the amine-modified polyethylene glycol Gd coordination compound amine-modified with DOPA of DOPA is more than 1.5:1.Research finds that when less than the ratio, obtained particle size is excessive；When more than 3:When 1, gadolinium contains in nano particle Amount can be less than the demand of radiography.More preferably, the amine-modified polyethylene glycol Gd coordination compound amine-modified with DOPA of DOPA feeds intake Mol ratio is 2:1.
Preferably, the molar concentration of the amine-modified Gd coordination compound of the DOPA is 1~2mM.
Copper ion concentration and temperature conditionss are main influence factors in the oxidative coupling reaction of the present invention, research hair Existing, when copper ion concentration is more than 40mM or less than 10mM, there is the problem of oversized or distribution is wide in nano material.Make To be preferred, the concentration of the copper ion is 15-25mM.More preferably, the concentration of the copper ion is 20mM.
Research has shown that heating-up temperature is more likely formed the good nano particle of size between 50-90 DEG C.Preferably, institute State to be heated to be at 50-80 DEG C and react 5-8h.More preferably, it is described be heated to be at 80 DEG C react 5h.
The preparation method of the present invention also includes：Reaction terminates, and retains the nano material using dialysis membrane, then freezed Dry.
The dialysis membrane is regenerated cellulose film, its pore size and selected methoxypolyethylene glycol-dopamine Molecular size range is adapted.
Present invention also offers a kind of nano material being prepared by above-mentioned preparation method, in the nano material gadolinium from The mass percentage content of son is 4~6%.The gadolinium concentrations of nano material need to reach a certain amount of and can be only achieved contrasting effects.
Nano material produced by the present invention can be stabilized more than two weeks in water, PBS and DMEM culture mediums, its particle diameter In 120nm or so, color is in black.
Small molecule gadolinium contrast agent and polyethylene glycol conjugation are formed macromolecular gadolinium contrast agent by the present invention, and its relaxation rate is reachable 9.55Mm-1·S-1, hence it is evident that higher than the relaxation rate of common contrast agent, there are more preferable contrasting effects, and macromolecular can be passed through The EPR effects of contrast agent are enriched in tumor tissues, improve MRI sensitivity, lift the diagnostic level of early-stage cancer.
Nano material prepared by the present invention has a certain amount of absorption in the near infrared region that wavelength is 650-950nm, grinds Study carefully discovery, the nano material of the present invention is applied in tumour cell, under the conditions of near infrared light photograph, tumour can be significantly inhibited The propagation of cell, therefore, can be as photo-thermal therapy agent.Photo-thermal therapy (PPT) is to utilize to have high light hot-cast socket effect The material of rate converts light energy into heat energy under near infrared light photograph, causes tumour cell localized hyperthermia, so as to kill cancer cell A kind for the treatment of method.
The beneficial effect that the present invention possesses：
(1) small molecule gadolinium contrast agent is transformed into macromolecular gadolinium contrast agent by the present invention by oxidative coupling reaction, is had and is relaxed Henan efficiency high, the advantages that circulation time in vivo is long, targeting and toxic side effect are small.
(2) nano material produced by the present invention has high light thermal transition efficiency near infrared region, can be used as photo-thermal Therapeutic agent is applied in photo-thermal therapy, determines position, size and the light treatment agent of tumour swollen by mr techniques in addition Enrichment condition in tumor tissue, for evaluating therapeutic effect, realize the photo-thermal therapy diagnosis and treatment integration of magnetic resonance imaging mediation.
Brief description of the drawings
Fig. 1 is dynamic light scattering diagram of the nano material in water in embodiment.
Fig. 2 is nano material electron microscope in embodiment.
Fig. 3 is the relation of nano material magnetic resonance contrast agent relaxation rate and time in DTPA-Gd and embodiment.
Fig. 4 is the external magnetic resonance imaging figure of nano material in deionized water, DTPA-Gd and embodiment, wherein, A：Deionization Water, B：DTPA-Gd, C：Nano material in embodiment；Gd in B and C2+Concentration be 0.1mM.
Fig. 5 is absorption figure of the nano material in embodiment near infrared region.
It only in optical power density is 1W/cm that Fig. 6, which is,2808nm near infrared lights shine under, illumination confrontation 4T1 cells, A549 are thin Born of the same parents and BCap-37 cell proliferation design sketch.
Fig. 7 be under without laser radiation situation, the nano material confrontation 4T1 cells of embodiment synthesis, A549 cells and BCap-37 cell proliferation design sketch.
Fig. 8 is that the 808nm near infrared lights that optical power density is 1W/cm2 shine 0s, when 10s, 30s, the nanometer of embodiment synthesis Material resistant 4T1 cell proliferation design sketch.
Fig. 9 is that the nano material of embodiment synthesis strengthens tumour magnetic resonance imaging figure as magnetic resonance contrast agent, wherein the One behavior DTPA-Gd, the second behavior embodiment nano materials.
Figure 10 is embodiment nano materials in the Inhibition test of 4T1 breast cancer cell lotus knurl BALB/C mice tumours Tumor growth curve figure.
Figure 11 is Inhibition test mistake of the embodiment nano materials to 4T1 breast cancer cell lotus knurl BALB/C mice tumours Cheng Zhong, BALB/C mice changes of weight curve map.
Embodiment
With reference to specific embodiment, the invention will be further described.
Embodiment 1
1st, the preparation of nano material
(1) mPEG-DA (131.09mg are added in 25mL deionized waters；2eq), DTPA (HTA)2@Gd(25mg；1eq), CuSO4·5H2O (125mg), makes Cu in system2+Concentration be 20mM, react 5h at 80 DEG C.
(2) dialyse (Mw=2000) 72h.
(3) freeze.
2nd, the performance evaluation of nano material
As shown in figure 1, the volume average particle size that dynamic light scattering (DLS) measures nano material is 115nm (breadth coefficients PDI=0.235).
As shown in Fig. 2 transmission electron microscope (TEM) observes that the particle diameter of nano material is 100nm, the particle diameter knot measured with DLS Fruit is consistent.
As shown in figure 3, after measured, the R1 values of magnevist are 4.3mM-1·s-1, it is coincide with document.The gadolinium of nano material Content is 4%, and relaxation rate R1 values are 9.55mM-1·s-1, it is 2.2 times of magnevist.
As shown in figure 4, the signal value of water is very weak, image is very dim, and magnevist can effectively strengthen its signal Intensity.Compared with magnevist, image when nano material is used as contrast agent is brighter, it is thus possible to shows more superior imaging Efficiency.
As shown in figure 5, nano material has very strong absorption near infrared region.
As shown in fig. 6, not adding nano material, only laser is irradiated, and the cell survival rate after 5 minutes is said more than 85% Bright only laser irradiates 5 minutes not to have a significant impact to tumor cell proliferation.
As shown in fig. 7,3 kinds of cell survival rates are all higher than 80% when concentrations of nanoparticles is less than 100ug/ml, illustrate this Nano material does not have a significant impact to the multiplication capacity of cell, it was demonstrated that material cytotoxicity itself is low.
As shown in figure 8,3 kinds of cells with 808nm irradiate 0s, 10s respectively after being incubated altogether with nano material respectively 24 hours, There is difference in 30s, the relative activity of cell.Wherein, when the concentration of nano material is 100ug/ml, no laser irradiation group it is thin The survival rate of born of the same parents is higher than 85%；Laser irradiates 30s, and its cell survival rate is less than 35%, illustrates that the nano material has applied to light The potentiality of heat cure.
As shown in figure 9, nano particle in imaging experiment the same as compared with current medical contrast medium magnevist, showing in vivo More longlasting contrast time is shown, also, in 20min, its signal intensity is 2 times of magnevist.
As shown in Figure 10, after treatment once, the tumour of three control groups (PBS groups, an administration group, an irradiation group) shows Gone out identical tumor growth rate, illustrate only be medicine or illumination in itself, the growth of tumour can not be contained.Experimental group The tumour of (administration+irradiation) disappears after laser irradiation, was not recurred in ensuing 15 days, illustrates that the nano material can Photo-thermal therapy to be applied to tumour as photothermal reagent.
As shown in figure 11, the mouse weight of four groups (2 weeks) during treatment changes without conspicuousness, illustrates nanometer Grain does not have conspicuousness toxicity to mouse.
Embodiment 2
Nano material is prepared according to the reaction condition of table 1, technological process is with reference to embodiment 1, obtained particle size result It is shown in Table 1.
Table 1
A：The amine-modified DTPA-Gd of DOPA
B：The amine-modified PEG of DOPA
As seen from the above table, when copper ion concentration is more than 40mM or less than 10mM, nano material exists oversized Problem.Excessive particle can not be enriched in tumor tissues through EPR effects, and is easily retained by reticuloendothelial system, therefore, The magnetic resonance imaging contrast and photo-thermal therapy agent for being not suitable as the present invention use.
For heating-up temperature more than 90 DEG C, there is the problem of particle diameter is uneven in the nano material of preparation, influence its using effect.

Claims (10)

  1. A kind of 1. preparation method for the nano material for being used for magnetic resonance imaging and/or photo-thermal therapy, it is characterised in that including： In the presence of copper ion, the amine-modified Gd coordination compound of DOPA and the amine-modified polyethylene glycol of DOPA are in heating condition in aqueous medium Under, the partial oxidation Viability higher semiquinone or quinone of catechol, product is spontaneous to agglomerate into described nano material.
  2. 2. preparation method as claimed in claim 1, it is characterised in that the structural formula of the amine-modified Gd coordination compound of the DOPA is such as Shown in formula (I)：
  3. 3. preparation method as claimed in claim 1, it is characterised in that the amine-modified polyethylene glycol structures formula such as formula of the DOPA (II) shown in：
  4. 4. preparation method as claimed in claim 1, it is characterised in that the amine-modified polyethylene glycol of the DOPA is that molecular weight is 2000-4000Da methoxypolyethylene glycol-dopamine.
  5. 5. preparation method as claimed in claim 1, it is characterised in that the amine-modified polyethylene glycol of DOPA and DOPA are amine-modified The molar ratio of Gd coordination compound is more than 1.5:1.
  6. 6. preparation method as claimed in claim 5, it is characterised in that the molar concentration of the amine-modified Gd coordination compound of the DOPA For 1-2mM.
  7. 7. preparation method as claimed in claim 5, it is characterised in that the concentration of the copper ion is 15-25mM.
  8. 8. preparation method as claimed in claim 1, it is characterised in that it is described be heated to be at 50-80 DEG C react 5-8h.
  9. 9. preparation method as claimed in claim 1, it is characterised in that also include：The nano material is retained using dialysis membrane, It is freeze-dried again.
  10. 10. a kind of nano material being prepared by any one of the claim 1-9 preparation methods, it is characterised in that described The mass percentage content of gadolinium ion is 4~6% in nano material.
CN201710812141.7A 2017-09-11 2017-09-11 Nano material for magnetic resonance imaging and/or photothermal therapy and preparation method thereof Active CN107469094B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710812141.7A CN107469094B (en) 2017-09-11 2017-09-11 Nano material for magnetic resonance imaging and/or photothermal therapy and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710812141.7A CN107469094B (en) 2017-09-11 2017-09-11 Nano material for magnetic resonance imaging and/or photothermal therapy and preparation method thereof

Publications (2)

Publication Number Publication Date
CN107469094A true CN107469094A (en) 2017-12-15
CN107469094B CN107469094B (en) 2020-05-22

Family

ID=60584263

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710812141.7A Active CN107469094B (en) 2017-09-11 2017-09-11 Nano material for magnetic resonance imaging and/or photothermal therapy and preparation method thereof

Country Status (1)

Country Link
CN (1) CN107469094B (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108478811A (en) * 2018-03-31 2018-09-04 湖北大学 A kind of high performance photo-thermal diagnosis Integral rice grain and preparation method thereof
CN110251692A (en) * 2019-06-14 2019-09-20 浙江大学 A kind of diagnosis and treatment integration nano material and the preparation method and application thereof
CN112876677A (en) * 2020-12-29 2021-06-01 浙江大学杭州国际科创中心 Dopamine functional poly (beta-amino ester) and preparation method and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150139914A1 (en) * 2012-04-09 2015-05-21 Snu R&Db Foundation Contrast-Enhancing Agent for Nuclear Magnetic Resonance Imaging Comprising Melanin Nanoparticles Stably Dispersed in Water

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150139914A1 (en) * 2012-04-09 2015-05-21 Snu R&Db Foundation Contrast-Enhancing Agent for Nuclear Magnetic Resonance Imaging Comprising Melanin Nanoparticles Stably Dispersed in Water

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
AARON M. MOHS ET AL.: "Modification of Gd-DTPA Cystine Copolymers With PEG-1000 Optimizes Pharmacokinetics and Tissue Retention for Magnetic Resonance Angiography", 《MAGNETIC RESONANCE IN MEDICINE》 *
QIAOCHU LI ET AL.: "Controlling Hydrogel Mechanics via Bio-Inspired Polymer−Nanoparticle Bond Dynamics", 《ACS NANO》 *
SOPHIE LAURENT ET AL.: "Bis(phenylethylamide) Derivatives of Gd-DTPA as Potential Receptor-Specific MRI Contrast Agents", 《EUR. J. INORG. CHEM.》 *
ZHAO-HUA MIAO ET AL.: "Intrinsically Mn2+-Chelated Polydopamine Nanoparticles for Simultaneous Magnetic Resonance Imaging and Photothermal Ablation of Cancer Cells", 《ACS APPL. MATER. INTERFACES》 *
ZHENHUA CHEN ET AL.: "Biocompatible, Functional Spheres Based on Oxidative Coupling Assembly of Green Tea Polyphenols", 《J. AM. CHEM. SOC.》 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108478811A (en) * 2018-03-31 2018-09-04 湖北大学 A kind of high performance photo-thermal diagnosis Integral rice grain and preparation method thereof
CN108478811B (en) * 2018-03-31 2021-11-30 湖北大学 High-performance photo-thermal diagnosis and treatment integrated nano-particles and preparation method thereof
CN110251692A (en) * 2019-06-14 2019-09-20 浙江大学 A kind of diagnosis and treatment integration nano material and the preparation method and application thereof
CN112876677A (en) * 2020-12-29 2021-06-01 浙江大学杭州国际科创中心 Dopamine functional poly (beta-amino ester) and preparation method and application thereof
CN112876677B (en) * 2020-12-29 2022-05-06 浙江大学杭州国际科创中心 Dopamine functional poly (beta-amino ester) and preparation method and application thereof

Also Published As

Publication number Publication date
CN107469094B (en) 2020-05-22

Similar Documents

Publication Publication Date Title
Du et al. Engineered gadolinium-doped carbon dots for magnetic resonance imaging-guided radiotherapy of tumors
Jin et al. Ultra-small iron-gallic acid coordination polymer nanoparticles for chelator-free labeling of 64 Cu and multimodal imaging-guided photothermal therapy
Zhang et al. Graphene oxide-BaGdF5 nanocomposites for multi-modal imaging and photothermal therapy
Wang et al. Functionalized Cu 3 BiS 3 nanoparticles for dual-modal imaging and targeted photothermal/photodynamic therapy
Sun et al. In situ synthesis of graphene oxide/gold nanorods theranostic hybrids for efficient tumor computed tomography imaging and photothermal therapy
Wang et al. A theranostic nanoplatform: magneto-gold@ fluorescence polymer nanoparticles for tumor targeting T 1 & T 2-MRI/CT/NIR fluorescence imaging and induction of genuine autophagy mediated chemotherapy
Zhang et al. Gadolinium-chelate functionalized copper sulphide as a nanotheranostic agent for MR imaging and photothermal destruction of cancer cells
Kong et al. Recent advances of polyoxometalates in multi-functional imaging and photothermal therapy
CN107551279B (en) Ultra-small protein composite nanoparticle with near-infrared photothermal effect and multi-modal imaging function, and preparation method and application thereof
CN107899011B (en) Manganese and dopamine-based tumor diagnosis and treatment nano material and preparation method and application thereof
Wen et al. A promising dual mode SPECT/CT imaging platform based on 99m Tc-labeled multifunctional dendrimer-entrapped gold nanoparticles
Yin et al. Biocompatible folate-modified Gd 3+/Yb 3+-doped ZnO nanoparticles for dualmodal MRI/CT imaging
CN103721271A (en) Multifunctional nano probe for multimodal images and photothermal therapy of liver cancer and application of multifunctional nano probe
CN111330024B (en) Iron-based tumor diagnosis and treatment nano material based on hyaluronic acid and polyphenol as well as preparation method and application thereof
CN104689346B (en) For tumour MRI/CT imagings and multifunctional nano probe and the application of photo-thermal therapy
CN105435248A (en) Multi-functional bismuth selenide nanocomposite and preparation method and application thereof
CN107469079B (en) Preparation method of photodynamic therapeutic agent under guidance of T1-MRI imaging
CN107469094A (en) A kind of nano material for being used for magnetic resonance imaging and/or photo-thermal therapy and preparation method thereof
Shan et al. Polypyrrole-based double rare earth hybrid nanoparticles for multimodal imaging and photothermal therapy
CN104721841B (en) A kind of fluorescence CT bimodal image probes and preparation method thereof
Jian-Hua et al. Facile synthesis of biocompatible Fe3O4-based nanoparticles for pH-responsive dual-model magnetic resonance imaging-guided tumour eradication by photothermal therapy
Xue et al. Novel multifunctional theranostic nanoagents based on Ho 3+ for CT/MRI dual-modality imaging-guided photothermal therapy
CN108514642A (en) A kind of preparation method for extra small ferroso-ferric oxide/Jenner's popped rice that dendrimer is stablized
Song et al. A multifunctional nanoprobe based on europium (iii) complex–Fe 3 O 4 nanoparticles for bimodal time-gated luminescence/magnetic resonance imaging of cancer cells in vitro and in vivo
Cai et al. BSA-templated synthesis of Ir/Gd bimetallic oxide nanotheranostics for MR/CT imaging-guided photothermal and photodynamic synergistic therapy

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant