CN107455389A - A kind of biological pesticide preparation synergistic composition of prevention and control red fire ant - Google Patents
A kind of biological pesticide preparation synergistic composition of prevention and control red fire ant Download PDFInfo
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- CN107455389A CN107455389A CN201710833103.XA CN201710833103A CN107455389A CN 107455389 A CN107455389 A CN 107455389A CN 201710833103 A CN201710833103 A CN 201710833103A CN 107455389 A CN107455389 A CN 107455389A
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- muscardine
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
- A01N25/14—Powders or granules wettable
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/08—Alkali metal chlorides; Alkaline earth metal chlorides
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Abstract
The invention discloses a kind of biological pesticide preparation synergistic composition of prevention and control red fire ant.It includes muscardine and sodium chloride, and the number of beauveria bassiana spore and the grams ratio of sodium chloride are：(1~8) × 20,000,000,000：4.0~7.5g.The present invention is found by experiment that, muscardine and sodium chloride compounding have a significant synergistic effect, and prevention effect is very good, there is the advantages of efficient, quick to red fire ant, it is nontoxic to people and animals, it is free of contamination to soil and groundwater resources, and its safety, so as to both improve the insecticidal effect of muscardine, the drug-fast enhancing of red fire ant is delayed again, has reduced environmental pollution, so as to improve preventive effect, it is cost-effective, delay the generation of pest resistance to insecticide.
Description
Technical field：
The invention belongs to Solenopsis invicta field, and in particular to a kind of biological pesticide preparation synergistic combination of prevention and control red fire ant
Thing.
Background technology：
Muscardine：Muscardine is a kind of ascomycetous entomogenous fungi, and the high cryptogam of muscardine is nonpoisonous and tasteless, and no environment is dirty
Dye, has persistent infection power to insect, and insect can continuously contaminate propagation once infection.It is residual without agriculture, even if being stood using after muscardine
Any residues of pesticides will not also be caused by carving harvest product；Non-resistant, muscardine are connect by the body wall under field conditions (factors) with insect
Sense of touch dye is lethal, and insect will not produce any resistance to it, use year after year, effect more and more higher on the contrary；Regrowth, muscardine
Novel biopesticide contains live body fungi and spore, after applying field, by suitable temperature, humidity, can continue breeding life
It is long, strengthen insecticidal effect；High selectivity, beneficial insect, insect total number are killed different from what chemical pesticide was not distinguished between the enemy and ourselves, muscardine is special
One property is strong, the beneficial insect such as non-target organism such as ladybug, Chrysopa and food aphid horsefly is influenceed smaller.Muscardine is because of above characteristic and by people
It is known as the environment friendly agricultural of 21 century, preferable agricultural chemicals.Therefore, develop, production biological pesticide undoubtedly has social effect concurrently as one kind
The advanced behavior of benefit and economic benefit.Develop both can safely and effectively be controlled using biological agent preventing and treating invasive species red fire ant
Insect, it can preserve the ecological environment again and exempt from destruction.
Sodium chloride (Sodium chloride)：Sodium chloride is a kind of colourless cubic crystal or fine crystalline powder, outward appearance are in
White crystalline, seawater is mainly derived from, is the main component of salt.Sodium-chloride water solution is in neutrality, property stable in the air
It is relatively good, medically it is used for configuring physiological saline, it is in life to can be used for flavouring, it is industrial general using electrolysis saturated sodium-chloride
Method next life hydrogen producing, chlorine and the caustic soda (sodium hydroxide) of solution and other chemical products (commonly referred to as chlorine industry),
Available for ore smelting (electrolyzing fused sodium chloride crystal produces active metal sodium).Therefore, if using sodium chloride as synergy
Agent add biological agent in have many advantages, such as, first, sodium chloride has very high security to people and animals, to dispenser operating personnel with
Applying area resident safety, and warding off property of drive will not be caused to red fire ant.Secondly, sodium chloride is environmentally safe, will not pollute air,
Very little is influenceed to caused by environment.Finally, sodium chloride there is no influence to fish and other aquatiles.
It is external to carry out already using (1994) such as the research, Stimac etc. (1993) of muscardine preventing and treating red fire ant and Oi successively
It has studied various concentrations with field indoors respectively, the control feelings of the muscardine of different administration method in ant nest to red fire ant
Condition.Therefore disease fungus provides hope (Liu Xiaoyan etc., 2006) for the sustainable control of red fire ant.Muscardine is considered as simultaneously
It is one of more promising pathogenic microorganism.When being due to so that muscardine is applied in the wild, due to temperature, humidity, ultraviolet light etc.
The limitation of environmental condition so that the germination rate of spore is relatively low, so that have impact on the insecticidal activity of muscardine, therefore, is administered alone
The speed of pathogenic microorganism muscardine preventing and treating red fire ant is relatively slow.And mixed by the pharmaceutical preparation of two or more component
It can effectively overcome disadvantage mentioned above with component.Meanwhile using synergistic effect caused by two kinds of drug component mixtures, it can improve
Preventive effect, construction times are reduced, it is cost-effective, accelerate the speed that insect is effectively controlled.
So far there are no report that insecticide that muscardine combines with sodium chloride applies on prevention and control red fire ant.
The content of the invention：
It is an object of the invention to provide a kind of biological pesticide preparation synergistic composition of safe and efficient prevention and control red fire ant.
The biological pesticide preparation synergistic composition of the prevention and control red fire ant of the present invention, it is characterised in that including muscardine and chlorine
Change sodium, the number of beauveria bassiana spore and the grams ratio of sodium chloride are：(1~8) × 20,000,000,000：4.0~7.5g.
The biological pesticide preparation synergistic composition of described prevention and control red fire ant, preferably water-dispersible granules, by quality point
Number 100% is counted, including the total mass fraction of muscardine pulvis and sodium chloride is 30%, and muscardine pulvis and sodium chloride mass ratio are
5：4.0~7.5g, described muscardine pulvis contain 20,000,000,000/g of beauveria bassiana spore, wetting agent pull open powder 8%, dispersant wood
Quality sodium sulfonate 18%, disintegrant calcium chloride 5%, stabilizer disodium hydrogen phosphate 2%, adhesive Sodium Polyacrylate 5% and carrier
Diatomite 32%.
It is preferred that the number of described beauveria bassiana spore and the grams ratio of sodium chloride are：5 × 20,000,000,000：4.38~7.30g.
More preferably：5 × 20,000,000,000：7.30g.
The biological pesticide preparation synergistic composition of described prevention and control red fire ant, it is best when water-dispersant solution is made
It is that the number that water-dispersant solution contains beauveria bassiana spore is 1 × 108Individual/mL-1With 75~125mmol/L of sodium chloride.
The present invention is found by experiment that muscardine and sodium chloride compounding have significant synergistic effect, and prevention effect is very good,
There is the advantages of efficient, quick to red fire ant, it is nontoxic to people and animals, it is free of contamination to soil and groundwater resources, and its safety, from
And the insecticidal effect of muscardine had both been improved, and delay the drug-fast enhancing of red fire ant again, reduced environmental pollution, it is anti-so as to improve
Effect, it is cost-effective, delay the generation of pest resistance to insecticide.
Brief description of the drawings：
Fig. 1 is red fire ant cumulative mortality figure, wherein muscardine after the lower 96h of the various concentrations medicine of embodiment 1 effect：It is white stiff
The bacterium 5g/L aqueous solution；1：Muscardine+25mmol/L NaCl；2：Muscardine+75mmol/L NaCl；3：Muscardine+100mmol/L
NaCl；4：Muscardine+125mmol/L NaCl；5：Muscardine+150mmol/L NaCl；6：Muscardine+200mmol/L NaCl；
7：Muscardine+250mmol/L NaCl；8：Muscardine+300mmol/L NaCl；CK：Sterilized water；NaCl：125mmol/L NaCl
The aqueous solution.
Fig. 2 is red fire ant cumulative mortality figure after the lower 96h of the different muscardine sodium chloride water dispersant effects of embodiment 2,
Wherein muscardine：Muscardine group；1：Muscardine+25mmol/L NaCl+ auxiliary materials；2：Muscardine+75mmol/L NaCl+ auxiliary materials；
3：Muscardine+100mmol/L NaCl+ auxiliary materials；4：Muscardine+125mmol/L NaCl+ auxiliary materials；5：Muscardine+150mmol/L
NaCl+ auxiliary materials；6：Muscardine+200mmol/L NaCl+ auxiliary materials；7：Muscardine+250mmol/L NaCl+ auxiliary materials；8：Muscardine+
300mmol/L NaCl+ auxiliary materials；CK：Sterilized water+auxiliary material；NaCl：The 125mmol/L NaCl aqueous solution+auxiliary material.
Embodiment：
Muscardine is to apply more a kind of insect pathogenic fungus, and there are sale, such as Guangdong Province in major DSMZ
Culture Collection, therefore those skilled in the art can go to major DSMZ to buy, and can also go business
Produce company's purchase of muscardine, such as Jiangxi Tianren Ecology Co., Ltd..
Following examples are to further explanation of the invention, rather than limitation of the present invention.
Embodiment 1：Muscardine compounds with sodium chloride and the Toxicity Determination of red fire ant worker ant is tested
During Toxicity Determination, weigh muscardine pulvis 5g and be dissolved in 1L water, be well mixed, obtain muscardine solution.Institute
With muscardine pulvis 20,000,000,000/g containing beauveria bassiana spore.9 parts of each 100ml muscardine solution is measured from 1L muscardine solution,
Then NaCl is added respectively inside respectively so that NaCl concentration is respectively in 9 parts of muscardine solution：0mmol/L,25mmol/
L, 75mmol/L, 100mmol/L, 125mmol/L, 150mmol/L, 200mmol/L, 250mmol/L, 300mmol/L, order
To muscardine sodium chloride water dispersion solution, the wherein spore concentration of muscardine is 1 × 108Individual/mL-1。
Muscardine solution, muscardine sodium chloride water dispersion solution, sodium chloride solution (125mmol/L) and nothing are determined respectively
Virulence of the bacterium water to healthy red fire ant worker ant.Each 3 repetitions of processing, each repeat 1 culture dish, and each culture dish puts 100
Head red fire ant worker ant.Take out, be put into after drying indoors in rearing-box, daily after red fire ant worker ant is immersed into decoction about 5s first
Raise with fresh honey, raised under the conditions of being placed in 26 ± 1 DEG C, humidity 70-90%, photoperiod 14L/10hD, 96h is observed after medicine
The survival condition of red fire ant worker ant, calculate the death rate.Data statistics uses SPSS13.0 softwares, with Duncan ' s methods to each sample
Between carry out significance test, the cumulative mortality significant difference (P ＜ 0.05) of red fire ant after different lowercase letters processing.
As a result as shown in figure 1, it will be seen from figure 1 that when be used alone NaCl processing red fire ant 96h after cumulative mortality
Difference is not notable compared with blank control both sterile water process red fire ant (15.91 ± 0.72%), illustrates to be used alone at NaCl
Reason does not have any killing effect to red fire ant；Cumulative mortality when muscardine is used alone after red fire ant 96h is with individually making
With NaCl and blank control significant difference, illustrate that muscardine, which is used alone, has certain insecticidal activity, but the death rate is relatively
It is low；But when sodium chloride is with 75mmol/L, 100mmol/L, when 125mmol/L concentration mixes with muscardine, after red fire ant 96h
Cumulative mortality is with being used alone muscardine and the equal significant difference (ie in solution of cumulative mortality of red fire ant after NaCl being used alone
Contain 1 × 108Individual/mL-1, 75~125mmol/L), absolutely prove and post-processed when sodium chloride and muscardine are mixed with certain proportion
Red fire ant can substantially increase the insecticidal effect of medicament, hence it is demonstrated that the insecticidal effect of the mixture is compared to independent sodium chloride or white
There is obvious synergistic effect during stiff bacterium use.
The present inventor does above-mentioned identical reality with calcium chloride, potassium chloride, magnesium chloride, ammonium sulfate, iron chloride instead of sodium chloride
Test, as a result find, calcium chloride, potassium chloride, magnesium chloride, ammonium sulfate, iron chloride do not have synergistic effect, and it adds and be not added with dialogue stiff
The influential effect for killing red fire ant of bacterium is little, without significant difference.
Embodiment 2：Muscardine sodium chloride water dispersant is tested to the Toxicity Determination of red fire ant worker ant
Weigh muscardine pulvis and sodium chloride amounts to 30g (wherein muscardine pulvis and sodium chloride mass ratio is respectively：5:
1.46；5:4.38；5:5.84；5:7.30；5:8.77；5:11.69；5:14.61g；5:17.53, muscardine pulvis used is containing white
Stiff 20,000,000,000/g of bacterium spore.), wetting agent pull open powder 8g, dispersant sodium lignin sulfonate 18g, disintegrant calcium chloride 5g, stably
Agent disodium hydrogen phosphate 2g, adhesive Sodium Polyacrylate 5g, carrier diatomaceous earth 32g, above raw material press the preparation of water-dispersible granules
The mixing of method input (1, according to formula raw material is well mixed；2nd, it is sanded and detects qualified laggard through sand mill after is well mixed
Row mist projection granulating；3rd, sieved after mist projection granulating by vibratory sieve, profile defective work returns to sand mill and is sanded again, outside
Shape certified products further quality testing, if product off quality also return to sand mill and are sanded again；4th, up-to-standard product are detected,
Packed；5th, the packaged water dispersible granules are put in storage), the muscardine sodium chloride water of mass fraction 30% is finally made
Dispersant.
Above-mentioned muscardine sodium chloride water dispersant is added to the water, it is molten to obtain muscardine sodium chloride water dispersant
Liquid.The spore concentration for making muscardine in each muscardine sodium chloride water-dispersant solution is 1 × 108Individual/mL-1, NaCl concentration
Respectively：25mmol/L (1 in corresponding diagram 2), 75mmol/L (2 in corresponding diagram 2), 100mmol/L (3 in corresponding diagram 2),
125mmol/L (4 in corresponding diagram 2), 150mmol/L (5 in corresponding diagram 2), 200mmol/L (6 in corresponding diagram 2),
250mmol/L (7 in corresponding diagram 2), 300mmol/L (8 in corresponding diagram 2).
With containing 1 × 108Individual mL-1The aqueous solution (muscardine solution) of beauveria bassiana spore also contains as compareing in the aqueous solution
With the auxiliary materials of above-mentioned experimental group same concentrations (wetting agent pull open powder, dispersant sodium lignin sulfonate, disintegrant calcium chloride, stably
Agent disodium hydrogen phosphate, adhesive Sodium Polyacrylate, carrier diatomaceous earth), it is named as muscardine group (muscardine in corresponding diagram 2).
Using concentration be 125mmol/L sodium-chloride water solutions as control, also containing identical with above-mentioned experimental group in the aqueous solution
The auxiliary material of concentration (wetting agent pull open powder, dispersant sodium lignin sulfonate, disintegrant calcium chloride, stabilizer disodium hydrogen phosphate, coheres
Agent Sodium Polyacrylate, carrier diatomaceous earth).It is named as NaCl groups (NaCl in corresponding diagram 2).
And individually sterilized water as (CK) is compareed, also contains (wet with the auxiliary material of above-mentioned experimental group same concentrations in water
Moisten agent pull open powder, dispersant sodium lignin sulfonate, disintegrant calcium chloride, stabilizer disodium hydrogen phosphate, adhesive Sodium Polyacrylate,
Carrier diatomaceous earth), it is named as CK groups (CK in corresponding diagram 2).
Muscardine solution (muscardine group), muscardine sodium chloride water-dispersant solution, sodium chloride solution are determined respectively
The virulence of (NaCl groups) and sterilized water (CK) to healthy red fire ant worker ant.Each 3 repetitions of processing, 1 culture dish is each repeated,
Each culture dish puts 100 red fire ant worker ants.Take out after red fire ant worker ant is immersed into decoction about 5s first, put after drying indoors
Enter in rearing-box, raise with fresh honey, raised under the conditions of being placed in 26 ± 1 DEG C, humidity 70-90%, photoperiod 14L/10hD daily
Support, 96h observes the survival condition of red fire ant worker ant after medicine, calculates the death rate.Data statistics uses SPSS13.0 softwares, uses
Duncan ' s methods are carrying out significance test each sample, the cumulative mortality of red fire ant is poor after different lowercase letter processing
Different notable (P ＜ 0.05).
As a result as shown in Fig. 2 figure it is seen that when be used alone NaCl processing red fire ant 96h after cumulative mortality
Difference is not notable compared with blank control both sterile water process red fire ant, illustrates that NaCl processing is used alone does not appoint to red fire ant
What killing effect；Cumulative mortality when muscardine is used alone after red fire ant 96h is with being used alone NaCl and blank control
Significant difference, illustrate that muscardine, which is used alone, has certain insecticidal activity, but the death rate is relatively low；But when sodium chloride with
When 75mmol/L, 100mmol/L, 125mmol/L concentration mix with muscardine, after red fire ant 96h cumulative mortality with individually
Using the equal significant difference of cumulative mortality of red fire ant after muscardine and exclusive use NaCl, absolutely prove when sodium chloride and white deadlock
Bacterium mixes post processing red fire ant with certain proportion can substantially increase the insecticidal effect of medicament, hence it is demonstrated that the desinsection of the mixture
Effect has obvious synergistic effect when comparing independent sodium chloride or muscardine use.
In addition figure it is seen that the desinsection of muscardine sodium chloride water dispersant is made in muscardine, sodium chloride and auxiliary material
Effect is suitable with the muscardine sodium chloride water dispersion solution effect for being not added with auxiliary material.
Embodiment 3：
During Toxicity Determination, weigh muscardine pulvis 1g and sodium chloride 7.5g is dissolved in 1L water, be well mixed, obtain white
Stiff bacterium solution.Muscardine pulvis used 20,000,000,000/g containing beauveria bassiana spore.
Toxicity Determination is carried out according to the method for embodiment 1, the results showed that, the muscardine solution of the present embodiment is to flourishing
Ant has preferable killing effect.
Embodiment 4：
During Toxicity Determination, weigh muscardine pulvis 8g and sodium chloride 4.0g is dissolved in 1L water, be well mixed, obtain white
Stiff bacterium solution.Muscardine pulvis used 20,000,000,000/g containing beauveria bassiana spore.
Toxicity Determination is carried out according to the method for embodiment 1, the results showed that, it has preferably to kill to red fire ant imitates
Fruit.
Claims (4)
1. a kind of biological pesticide preparation synergistic composition of prevention and control red fire ant, it is characterised in that including muscardine and sodium chloride, in vain
The number of stiff bacterium spore and the grams ratio of sodium chloride are：(1~8) × 20,000,000,000：4.0~7.5g.
2. the biological pesticide preparation synergistic composition of prevention and control red fire ant according to claim 1, it is characterised in that described
The biological pesticide preparation synergistic composition of prevention and control red fire ant is water-dispersible granules, based on mass fraction 100%, including muscardine
The total mass fraction of pulvis and sodium chloride is 30%, and muscardine pulvis and sodium chloride mass ratio are 5：4.0~7.5g, described is white
Stiff bacteria powder contains 20,000,000,000/g of beauveria bassiana spore, wetting agent pull open powder 8%, dispersant sodium lignin sulfonate 18%, disintegrant
Calcium chloride 5%, stabilizer disodium hydrogen phosphate 2%, adhesive Sodium Polyacrylate 5% and carrier diatomaceous earth 32%.
3. the biological pesticide preparation synergistic composition of prevention and control red fire ant according to claim 1 or 2, it is characterised in that institute
The number for the beauveria bassiana spore stated and the grams ratio of sodium chloride are：5 × 20,000,000,000：4.38~7.30g.
4. the biological pesticide preparation synergistic composition of prevention and control red fire ant according to claim 3, it is characterised in that described
The number of beauveria bassiana spore and the grams ratio of sodium chloride are：5 × 20,000,000,000：7.30g.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710833103.XA CN107455389B (en) | 2017-09-15 | 2017-09-15 | Synergistic composition of biopesticide preparation for preventing and controlling red imported fire ants |
| PCT/CN2017/116135 WO2019052047A1 (en) | 2017-09-15 | 2017-12-14 | Biopesticide preparation synergistic composition for preventing and controlling solenopsis invicta |
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| CN201710833103.XA CN107455389B (en) | 2017-09-15 | 2017-09-15 | Synergistic composition of biopesticide preparation for preventing and controlling red imported fire ants |
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| WO2019052047A1 (en) * | 2017-09-15 | 2019-03-21 | 广东省生物资源应用研究所 | Biopesticide preparation synergistic composition for preventing and controlling solenopsis invicta |
| WO2019052048A1 (en) * | 2017-09-15 | 2019-03-21 | 广东省生物资源应用研究所 | Method for preparing biopesticide preparation for preventing and controlling solenopsis invicta |
| CN115413653A (en) * | 2022-10-10 | 2022-12-02 | 华南农业大学 | Tetrafluoromethyllum decastes powder for preventing and treating solenopsis invicta |
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| CN114617125B (en) * | 2021-11-24 | 2024-02-20 | 广东省林业科学研究院 | Application of 2-methoxy-3-methyl-1, 4-benzoquinone in prevention and treatment of solenopsis invicta and Monochamus alternatus |
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| WO2019052048A1 (en) * | 2017-09-15 | 2019-03-21 | 广东省生物资源应用研究所 | Method for preparing biopesticide preparation for preventing and controlling solenopsis invicta |
| US11350634B2 (en) | 2017-09-15 | 2022-06-07 | Institute of Zoology, Guangdong Academy of Sciences | Method for preparing biopesticide preparation for preventing and controlling Solenopsis invicta |
| CN115413653A (en) * | 2022-10-10 | 2022-12-02 | 华南农业大学 | Tetrafluoromethyllum decastes powder for preventing and treating solenopsis invicta |
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| WO2019052047A1 (en) | 2019-03-21 |
| CN107455389B (en) | 2020-04-14 |
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