CN107449775A - A kind of plant phenotype measurement apparatus and method - Google Patents
A kind of plant phenotype measurement apparatus and method Download PDFInfo
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- CN107449775A CN107449775A CN201710569488.3A CN201710569488A CN107449775A CN 107449775 A CN107449775 A CN 107449775A CN 201710569488 A CN201710569488 A CN 201710569488A CN 107449775 A CN107449775 A CN 107449775A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N5/00—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N2021/8466—Investigation of vegetal material, e.g. leaves, plants, fruits
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Abstract
The present invention, which provides a kind of plant phenotype measurement apparatus and method, wherein described device, to be included:Inflated airbag cover, rotate weigh box and sensor box;Wherein, the side of the air cell covering is opened wide;Box is weighed in the rotation and sensor box is located inside the air cell covering;The box of weighing that rotates includes clamping device, stepper motor, driver, controller and weighing device;The sensor box includes 3D cameras, spectrum camera and otherwise visible light color camera.By the present invention in that with inflated airbag cover, easy to remove and arrangement plant sample measuring environment, weighed in rotation and fix plant sample on box, rotate plant sample, and obtain the weight of plant sample;Meanwhile in rotary course, the image of the plant sample is obtained using sensor box, the phenotypic parameter value of plant sample is obtained according to the image of the plant sample so that phenotype measurement is economical, simple, efficiently and accurately.
Description
Technical field
The present invention relates to data collecting field, more particularly, to a kind of plant phenotype measurement apparatus and method.
Background technology
Plant phenotype research is an important topic of botany and field of biology, mainly passes through the various spies to plant
Character of the seeking peace i.e. discriminating of phenotype and analysis, and monitoring and control to plant growth environment deeply illustrate gene and environment because
Element is to the complexing action of plant phenotype, the correlation between plant phenotype and its yield, physiological status, and different environment
Influence of the condition to vegetation growth state, yield and quality etc..
At present, the research that the physiological every aspect of arable farming is acquired from functional genome is required for determining a large amount of plant
Different levels phenotypic characteristic and character, such as the body parameter and Physiological And Biochemical Parameters of plant.Wherein described body parameter bag
Include structure, density, leaf area, leaf long, leaf width and seed color, the Physiological And Biochemical Parameters include nutrient analysis, moisture distribution,
Water stress, transpiration, photosynthetic physiology state and pest and disease damage.Complete acquisition and analysis to the more phenotypic informations of plant have turned into
One important research direction of plant phenotype group.
Traditional phenotype measurement is completed by manual type, the problems such as efficiency is low, error is uncontrollable be present.In recent years,
Domestic and international scientific research institution and large enterprise have started to the research and development of high flux plant phenotype measuring table, as LemnaTec is public
Department and the plant phenotype measuring system of PSI companies.Although the plant phenotype measuring system can realize that batch plant is scanned, knot
Structure is complicated, cost is high, maintenance cost is high, and the limited amount that can detect sample is limited by place.
The content of the invention
To overcome that above-mentioned existing phenotype measurement apparatus is complicated, cost is high and the problem of limitation by place or at least
Partly solve the above problems, the invention provides a kind of plant phenotype measurement apparatus and method.
According to the first aspect of the invention, there is provided a kind of plant phenotype measurement apparatus, including:
Inflated airbag cover, rotate weigh box and sensor box;
Wherein, the side of the air cell covering is opened wide;
Box is weighed in the rotation and sensor box is located inside the air cell covering;
The box of weighing that rotates includes clamping device, stepper motor, driver, controller and weighing device;
Wherein, the stepper motor, driver and controller are located at below the clamping device;
The driver is connected with the stepper motor, and the controller is connected with the driver;
The weighing device is located at below the Step motor;
The sensor box includes 3D cameras, spectrum camera and otherwise visible light color camera.
Specifically, described device also includes setup module, and the setup module is connected with the controller, for setting
State the interval angles of plant sample rotation.
Specifically, the controller is used to rotate plant sample, until the plant sample is rotated by 360 °.
Specifically, the controller stops preset duration after being used to make the plant sample per rotation steps angles, and
The anglec of rotation of the plant sample is less than 360 degree and state when being halted state, to sensor box transmission trigger signal,
To trigger enabling for sensor in the sensor box.
Specifically, described device also includes color correction colour atla, for obtaining the Standard Colors of the plant sample.
Specifically, the 3D cameras are used for 60 degree of level, vertical 45 degree of rectangular pyramid shape transmitting laser spots, and capture
The image of the laser spots.
Specifically, the spectrum camera is provided with optical filter, and the spectral response range of the spectrum camera is 900-
2500nm, lens focus 8mm, resolution ratio 4000 × 3000.
Specifically, the lens focus of the otherwise visible light color camera is 12mm, and resolution ratio is 4000 × 3000.
According to the second aspect of the invention, there is provided a kind of plant phenotype measuring method, methods described use said apparatus, bag
Include:
After the root system for removing plant sample, the plant sample is fixed on the clamping device for rotating box of weighing;
The plant sample is rotated by the box of weighing that rotates, the weighing device in box of being weighed by the rotation obtains
Take the weight of the plant sample;
The form phenotypic parameter value of the plant sample is obtained by the 3D cameras in sensor box, passes through the sensor
Spectrum camera in box obtains physiological phenotype parameter value, and color line is obtained by the otherwise visible light color camera in the sensor box
Manage phenotypic parameter value.
Specifically, the plant sample stops preset duration after often rotating predetermined interval angle;
If judge that the anglec of rotation of the plant sample is less than 360 degree and state for halted state, to the sensor
Box sends trigger signal, to trigger enabling for sensor in the sensor box.
The present invention provides a kind of plant phenotype measurement apparatus and method, by using inflated airbag cover, it is easy to remove and
The measuring environment of plant sample is arranged, is weighed in rotation and fixes plant sample on box, rotate plant sample, and obtain plant sample
This weight;Meanwhile in rotary course, the image of the plant sample is obtained using sensor box, according to the plant sample
This image obtains the phenotypic parameter value of plant sample so that phenotype measurement is economical, simple, efficiently and accurately.
Brief description of the drawings
Fig. 1 is plant phenotype measurement apparatus structural representation provided in an embodiment of the present invention;
Fig. 2 is plant phenotype measuring method flow chart provided in an embodiment of the present invention.
Embodiment
With reference to the accompanying drawings and examples, the embodiment of the present invention is described in further detail.Implement below
Example is used to illustrate the present invention, but is not limited to the scope of the present invention.
A kind of plant phenotype measurement apparatus is provided in one embodiment of the invention, and Fig. 1 provides for the embodiment of the present invention
Plant phenotype measurement apparatus structural representation, the device includes:Air cell covering 1, rotate weigh box 2 and sensor box 3;Wherein,
The side of the air cell covering 1 is opened wide;Box 2 is weighed in the rotation and sensor box 3 is located inside the air cell covering 1;The rotation
Box 2 of weighing includes clamping device, stepper motor, driver, controller and weighing device;Wherein, the stepper motor, driver
It is located at controller below the clamping device;The driver is connected with the stepper motor, the controller and the drive
Dynamic device is connected;The weighing device is located at below the Step motor;The sensor box 3 include 3D cameras, spectrum camera and
Otherwise visible light color camera.
Specifically, the plant phenotype measurement apparatus includes air cell covering 1, rotates weigh box 2, sensor box 3.Wherein, institute
Stating on air cell covering 1 has air-filled pore, before starting to measure plant phenotype, can use air pump by air-filled pore to the air cell covering 1
It is inflated, the side of the air cell covering 1 is opened wide, and the air cell covering after inflation forms semi-enclosed plant phenotype measuring environment, keeps away
Exempt from disturbance of the wind of open air to plant leaf blade and stalk, while avoid converting influence of the illumination to shooting.The shape of the air cell covering
Shape can be square, cuboid etc., and the present embodiment is not limited to the shape of the air cell covering.After measurement, it can open
Air-filled pore, the gas in the air cell covering is released, is convenient for carrying.Box 2 is weighed in the rotation and sensor box 3 is located at the gas
Inside capsule cover 1.The sensor box 3 rotates box 2 of weighing towards described, to obtain the plant for rotating fixation on box 2 of weighing
The image of sample.The lower section of sensor box 3 can have support frame, for supporting the sensor box 3.Support frame as described above can be with
Regulation height.
The box 2 of weighing that rotates includes clamping device, stepper motor, driver, controller and weighing device.Wherein, institute
Clamping device is stated to be used to fix plant sample;The stepper motor, driver and controller are located at below the clamping device;Institute
Stepper motor is stated to be used to rotate the plant sample.The stepper motor directly can be connected with the clamping device or with
The part that the clamping device is fixed is connected.The driver is connected with the stepper motor, for driving the stepping electricity
Machine rotates.The controller is connected with the driver, and the driver is controlled by controller programming, so that the driver
The stepper motor is driven to rotate, so that being fixed on the plant sample rotation that rotation is weighed on box.The weighing device is located at
Below the Step motor, for weighing the weight of the plant sample.
The sensor box 3 includes 3D cameras, spectrum camera and otherwise visible light color camera.Wherein, the 3D cameras transmitting
Laser spots, after the laser spots are irradiated to the plant sample, the 3D cameras capture the image of the laser spots.By described in
Laser dot image passes to back-end server, and the laser dot image is three-dimensional point cloud.Server is according to the three-dimensional point cloud meter
Calculate the height of the plant sample, width, the number of blade, cauline leaf angle, azimuth, the form phenotypic parameter such as stalk is long, stalk is thick
Value.The spectrum camera obtains the spectrum picture of the plant sample, and the spectrum picture is passed into back-end server, takes
Device be engaged according to physiology tables such as the moisture of the spectrum picture calculating plant sample, chlorophyll content, protein contents
The value of shape parameter.The otherwise visible light color camera obtains otherwise visible light color image, after the otherwise visible light color image is passed to
Server is held, color, the value of texture isophenous parameter of the plant sample are obtained by the otherwise visible light color image.
The present embodiment easy to remove and arrangement plant sample measuring environment, is being rotated by using inflated airbag cover
Weigh and fix plant sample on box, rotate plant sample, and obtain the weight of plant sample;Meanwhile in rotary course, make
The image of the plant sample is obtained with sensor box, the phenotypic parameter of plant sample is obtained according to the image of the plant sample
Value so that phenotype measurement is economic, simple, efficiently and accurately.
On the basis of above-described embodiment, device described in the present embodiment also includes setup module, the setup module with
The controller is connected, for setting the interval angles of the plant sample rotation.
Specifically, the plant phenotype measurement apparatus also includes setup module, the setup module and the controller phase
Even, the setup module is used for the interval angles for setting the plant sample rotation.Setup module is by the angular interval of setting
Degree passes to the controller, and the controller controls the driver, so that the driver drives the stepper motor band
The plant sample is moved to rotate with the interval angles.
On the basis of any of the above-described embodiment, controller described in the present embodiment is used to rotate the plant, until
The plant sample is rotated by 360 °.
Specifically, the controller is used to control the driver, so that the driver drives the stepper motor band
The dynamic plant sample rotation.After the plant sample rotates to 360 degree, the plant sample is set to stop the rotation.Now, institute
State sensor box and got the image that the plant sample rotates a circle, if allowing the plant sample to continue to rotate, obtain
Be multiimage, waste of resource.
On the basis of any of the above-described embodiment, controller described in the present embodiment is used to make the plant sample often rotate
Stop preset duration after interval angles, and when the anglec of rotation of the plant sample is less than 360 degree and state is halted state,
Trigger signal is sent to the sensor box, to trigger enabling for sensor in the sensor box.
Specifically, the controller controls the driver, so that the driver drives the stepper motor to drive institute
Stop preset duration after plant sample is stated per rotation steps angle.After the preset duration, the plant sample is set to continue to revolve
Turn, stop after being rotated by 360 °.The controller judges whether the anglec of rotation of the plant sample is less than 360 degree and shape
Whether state is halted state.If it is determined that it is halted state that the anglec of rotation of the plant sample, which is less than 360 degree and state, then institute
State controller and send trigger signal to the sensor box, started working with triggering sensor in the sensor box.If sentence
The anglec of rotation of the disconnected plant sample is more than 360 degree and is halted state, then does not send trigger signal.If it is determined that the plant
The state of thing sample is rotation status, then does not also send trigger signal.
On the basis of any of the above-described embodiment, device described in the present embodiment also includes color correction colour atla, for obtaining
Take the Standard Colors of the plant sample.
Specifically, described device also includes color correction colour atla, and the color correction colour atla can be 128 color color corrections
Colour atla, the present embodiment are not limited to the species of the color correction colour atla.The color correction colour atla is placed on the plant sample
This side, enables the sensor box to obtain the image of the color correction colour atla and plant sample simultaneously, and in the image
Color correction colour atla and plant sample it is not overlapping.The standard face of the image is calculated using the color correction colour atla in the image
Color.
On the basis of any of the above-described embodiment, 3D cameras described in the present embodiment is used for 60 degree of level, vertical 45 degree
Rectangular pyramid shape transmitting laser spots, and capture the image of the laser spots.
Specifically, the 3D cameras can launch multiple laser spots, such as 800 × 600 laser spots.The laser spots are with water
Flat 60 degree vertical 45 degree rectangular pyramid shape projects.After the laser spots are irradiated to the plant sample, the 3D phases
Machine captures the image of the laser spots.More remote apart from the 3D cameras, the distance of adjacent laser point is bigger.Laser spots PnZ sit
Target computational methods are:
Z=α × D (Pn,Pn+1),
Wherein, α is coefficient, and D () is the distance between two neighboring laser spots in laser dot image.The factor alpha can be
Obtained by the demarcation of plane where Z coordinate.The laser spots PnX-coordinate computational methods be:X=Z × x/ α.Laser spots Pn's
Y-coordinate computational methods are:Y=Z × y/ α.Wherein, x is the abscissa of laser dot image, and y is the ordinate of laser dot image.
After the three-dimensional point cloud coordinate (X, Y, Z) for obtaining each laser spots, the form table of the plant sample can be obtained by points cloud processing
Shape parameter value.
On the basis of any of the above-described embodiment, spectrum camera described in the present embodiment is provided with optical filter, the spectrum
The spectral response range of camera is 900-2500nm, and lens focus 8mm, resolution ratio is 4000 × 3000.
Specifically, the spectrum camera is provided with optical filter, and the spectral response range of the spectrum camera is 900-
2500nm, lens focus 8mm, resolution ratio are 4000 × 3000.The spectrum camera can shoot the more of the plant sample
Individual spectrum channel image, including the chlorophyll characteristic wave bands image of plant, moisture characteristic wave bands image, protein characteristic ripple
Section image etc., the spectrum channel image can reflect nutrition condition, water deficit, the biochemical reactions of the plant sample
Active degree etc..
On the basis of any of the above-described embodiment, the lens focus of otherwise visible light color camera described in the present embodiment is
12mm, resolution ratio are 4000 × 3000.
Specifically, light color camera lens focus 12mm can described be seen, resolution ratio is 4000 × 3000, described for obtaining
The color and texture of plant sample.
A kind of plant phenotype measuring method is provided in another embodiment of the present invention, and Fig. 2 carries for the embodiment of the present invention
The plant phenotype measuring method flow chart of confession, the plant phenotype measurement apparatus described in this method the various embodiments described above, including it is following
Step:S1, after the root system for removing plant sample, by the plant sample be fixed on rotation weigh box clamping device on;S2, lead to
Crossing the box of weighing that rotates rotates the plant sample, passes through the weighing device rotated in box of weighing and obtains the plant
The weight of sample;S3, the form phenotypic parameter value of the plant sample is obtained by the 3D cameras in sensor box, by described
Spectrum camera in sensor box obtains physiological phenotype parameter value, is obtained by the otherwise visible light color camera in the sensor box
Color and vein phenotypic parameter value.
Specifically, in S1, before plant phenotype measurement is carried out, using air pump by the air-filled pore on air cell covering to air bag
Cover inflation, the air cell covering after inflation form semi-enclosed plant phenotype measuring environment, avoid the wind of open air to plant leaf blade and stem
The disturbance of stalk, while avoid converting influence of the illumination to shooting.The shape of the air cell covering can be square, cuboid etc.,
The present embodiment is not limited to the shape of the air cell covering.After measurement, air-filled pore can be opened, by the gas in the air cell covering
Body is released, and is convenient for carrying.Region inflated airbag cover being placed on where plant sample.Plant sample is obtained, described in removal
It is fixed in after the root system of plant sample on the clamping device for rotating box of weighing.In S2, the electricity for rotating box of weighing is connected
Source, rotate the plant sample.The weight of the plant sample is obtained by the weighing device rotated in box of weighing.
In S3, the sensor box includes 3D cameras, spectrum camera and otherwise visible light color camera.The sensor box direction
It is described to rotate box of weighing.The height of the sensor box can be adjusted, and by adjusting the height of the sensor box, obtain shooting
The suitable visual angle of the plant sample.Wherein, the 3D cameras transmitting laser spots, when the laser spots are irradiated to the plant sample
After this, the 3D cameras capture the image of the laser spots.The laser dot image is passed into back-end server, the laser
Dot image is three-dimensional point cloud.Server is according to the height of the three-dimensional point cloud calculating plant sample, width, the number of blade, stem
Leaf angle, azimuth, the value for the form phenotypic parameter such as stalk is long, stalk is thick.The spectrum camera obtains the plant sample
Spectrum picture, the spectrum picture is passed into back-end server, server calculates the plant sample according to the spectrum picture
The value of the physiological phenotype parameters such as moisture originally, chlorophyll content, protein content.The otherwise visible light color camera obtains can
See honorable color image, the otherwise visible light color image is passed into back-end server, obtained by the otherwise visible light color image
Color, the value of texture isophenous parameter of the plant sample.
The present embodiment easy to remove and arrangement plant sample measuring environment, is being rotated by using inflated airbag cover
Weigh and fix plant sample on box, rotate plant sample, and obtain the weight of plant sample;Meanwhile in rotary course, make
The image of the plant sample is obtained with sensor box, the phenotypic parameter of plant sample is obtained according to the image of the plant sample
Value so that phenotype measurement is economic, simple, efficiently and accurately.
On the basis of above-described embodiment, plant sample described in the present embodiment stops in advance after often rotating predetermined interval angle
If duration;If judge that the anglec of rotation of the plant sample is less than 360 degree and state for halted state, to the sensor box
Trigger signal is sent, to trigger enabling for sensor in the sensor box.
Specifically, preset duration is stopped after the plant sample is per rotation steps angle.After the preset duration, make institute
State plant sample to continue to rotate, stop after being rotated by 360 °.Judge whether the anglec of rotation of the plant sample is less than 360
Spend and whether state is halted state.If it is determined that the anglec of rotation of the plant sample is less than 360 degree and state is stopping shape
State, then trigger signal is sent to the sensor box, started working with triggering sensor in the sensor box.If it is determined that institute
The anglec of rotation for stating plant sample is more than 360 degree and be halted state, then does not send trigger signal.If it is determined that the plant sample
This state is rotation status, then does not also send trigger signal.
Finally, the present processes are only preferable embodiment, are not intended to limit the scope of the present invention.It is all
Within the spirit and principles in the present invention, any modification, equivalent substitution and improvements made etc., the protection of the present invention should be included in
Within the scope of.
Claims (10)
- A kind of 1. plant phenotype measurement apparatus, it is characterised in that including:Inflated airbag cover, rotate weigh box and sensor box;Wherein, the side of the air cell covering is opened wide;Box is weighed in the rotation and sensor box is located inside the air cell covering;The box of weighing that rotates includes clamping device, stepper motor, driver, controller and weighing device;Wherein, the stepper motor, driver and controller are located at below the clamping device;The driver is connected with the stepper motor, and the controller is connected with the driver;The weighing device is located at below the Step motor;The sensor box includes 3D cameras, spectrum camera and otherwise visible light color camera.
- 2. plant phenotype measurement apparatus according to claim 1, it is characterised in that described device also includes setup module, The setup module is connected with the controller, for setting the interval angles of the plant sample rotation.
- 3. plant phenotype measurement apparatus according to claim 1, it is characterised in that the controller is used to make plant sample Rotation, until the plant sample is rotated by 360 °.
- 4. plant phenotype measurement apparatus according to claim 3, it is characterised in that the controller is used to make the plant Sample per rotation steps angles after stop preset duration, and be less than 360 degree in the anglec of rotation of the plant sample and state is During halted state, trigger signal is sent to the sensor box, to trigger enabling for sensor in the sensor box.
- 5. plant phenotype measurement apparatus according to claim 1, it is characterised in that described device also includes color correction color Card, for obtaining the Standard Colors of the plant sample.
- 6. plant phenotype measurement apparatus according to claim 1, it is characterised in that the 3D cameras are used for level 60 Degree, vertical 45 degree of rectangular pyramid shape transmitting laser spots, and capture the image of the laser spots.
- 7. plant phenotype measurement apparatus according to claim 1, it is characterised in that the spectrum camera is provided with optical filtering Piece, the spectral response range of the spectrum camera are 900-2500nm, lens focus 8mm, resolution ratio 4000 × 3000.
- 8. plant phenotype measurement apparatus according to claim 1, it is characterised in that the camera lens of the otherwise visible light color camera Focal length is 12mm, and resolution ratio is 4000 × 3000.
- 9. a kind of plant phenotype measuring method, it is characterised in that usage right requires any described plant phenotype measurement dresses of 1-8 Put, comprise the following steps:After the root system for removing plant sample, the plant sample is fixed on the clamping device for rotating box of weighing;The plant sample is rotated by the box of weighing that rotates, passes through the weighing device rotated in box of weighing and obtains institute State the weight of plant sample;The form phenotypic parameter value of the plant sample is obtained by the 3D cameras in sensor box, by the sensor box Spectrum camera obtain physiological phenotype parameter value, color and vein table is obtained by otherwise visible light color camera in the sensor box Shape parameter value.
- 10. plant phenotype measuring method according to claim 9, it is characterised in that the plant sample often rotates default Stop preset duration after interval angles;If judge that the anglec of rotation of the plant sample is less than 360 degree and state for halted state, to sensor box hair Trigger signal is sent, to trigger enabling for sensor in the sensor box.
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CN109373977A (en) * | 2019-01-02 | 2019-02-22 | 山东农业大学 | The active vision platform and plant phenotype acquisition methods obtained for plant phenotype |
CN111179363A (en) * | 2020-01-14 | 2020-05-19 | 北京农业信息技术研究中心 | High-throughput plant phenotype measuring device and method |
CN112986240A (en) * | 2021-02-03 | 2021-06-18 | 南京农业大学 | Leaf phenotype-based tea tree variety identification method |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN109238166A (en) * | 2018-07-17 | 2019-01-18 | 北京农业信息技术研究中心 | A kind of plant phenotype acquisition device and its acquisition method |
CN109373977A (en) * | 2019-01-02 | 2019-02-22 | 山东农业大学 | The active vision platform and plant phenotype acquisition methods obtained for plant phenotype |
CN111179363A (en) * | 2020-01-14 | 2020-05-19 | 北京农业信息技术研究中心 | High-throughput plant phenotype measuring device and method |
CN111179363B (en) * | 2020-01-14 | 2023-09-12 | 北京农业信息技术研究中心 | High-throughput plant phenotype measuring device and method |
CN112986240A (en) * | 2021-02-03 | 2021-06-18 | 南京农业大学 | Leaf phenotype-based tea tree variety identification method |
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