CN107449771A - Enzyme-catalyzed chemical luminescence substrate - Google Patents
Enzyme-catalyzed chemical luminescence substrate Download PDFInfo
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- CN107449771A CN107449771A CN201710506220.5A CN201710506220A CN107449771A CN 107449771 A CN107449771 A CN 107449771A CN 201710506220 A CN201710506220 A CN 201710506220A CN 107449771 A CN107449771 A CN 107449771A
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- enzyme
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
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Abstract
The present invention discloses a kind of enzyme-catalyzed chemical luminescence substrate.The enzyme-catalyzed chemical luminescence substrate contains following component：The acridan disodium salt of 9 (4 chlorophenylthio benzoyl Oxymethylene) 10 methyl 9,10；5 (myristoyl amido) fluoresceins；The acridine derivatives of aryl acylhydrazone 9,10.In the present invention, the acridine derivatives of increase aryl acylhydrazone 9,10 are as substrate reinforcing agent, it is dissolved in substrate solution, can with alkaline phosphatase enzyme effect and light, make the enzyme-catalyzed chemical luminescence substrate have luminous signal it is strong, into plateau is very fast, duration length, the advantages that normal temperature long shelf-life.
Description
【Technical field】
The present invention relates to technical field of immunoassay, and in particular to a kind of enzyme-catalyzed chemical luminescence substrate.
【Background technology】
Chemiluminescence is a kind of material light radiation phenomenon adjoint in chemical reaction process is carried out, and can be divided into direct hair
Light and indirect light emission.Directly luminous is simplest chemiluminescence reaction, is made up of two committed steps：Excite and radiate.
C materials are generated as chemical reaction occurs for two kinds of materials of A, B, the energy of release is reacted by the molecule absorption of C materials and transits to sharp
State C* is sent out, light radiation is produced during ground state is returned in the C* excited.Here C* is illuminator, due to C during this
Reaction is directly participated in, therefore claims direct chemiluminescence.Indirect light emission is also known as energy transfer chemiluminescence, and it is mainly by three step groups
Into：Reactant A and B reaction generation excitation state intermediate C* (energy donor) first；Give off energy transfer when C* is decomposed
F (energy acceptor) is given, F is excited and transits to excitation state F*；Finally, when ground state is returned in F* transition, produce luminous.
Mainly on the basis of original luminescence reagent and system, research synthesizes new luminous examination for chemiluminescent latest development
Agent, new luminescence system is established, and other technologies, such as Flow Injection Technique (FIA), high performance liquid chromatography (HPLC), immobilization
Reagent technique, sensor technology and biochemical immunity technology etc. are combined, and have widened chemiluminescent application.Chemiluminescence with
Biochemical immunity analysis is combined, and has started the frontier of chemiluminescence analysis.Due to its on-radiation, high sensitivity and operation side
Just, it is raw for enzyme, atriphos (ATP), antigen, antibody, hormone etc. as an important means of biochemical immunity analysis
The analysis of active substances plays more and more important effect.
APS-5 is a kind of new chemical luminous substrate, and its application in the detection of homogeneous alkaline phosphatase shows superelevation
Sensitivity, (can detect less than 10-19Mol alkaline phosphatase), peak value can be rapidly reached to reduce detection time, increased
Add throughput, the slope of linear calibration curve is to be equal to 1.0 with log-log to draw.The enzyme of one quantity and the above produces one
The luminous quantity of quantity and the above, continuous illumination --- the requirement to minute is not high.Luminous intensity can be at any time
Read from caused linear calibration curve, analysis result insensitive, reduction control temperature in the range of 22 DEG C -35 DEG C to temperature
Accuracy needed for degree.APS-5 employs the technology of uniqueness, during chemiluminescence detection, is carried for alkaline phosphatase coupling
For superior sensitivity and ease for use.Reaction is used as substrate and alkali phosphatase enzyme mark by acr idan (9,10- acridan),
Production continues the chemiluminescence of high intensity.APS-5 is solution analysis method measure phosphatase activity and phosphatase enzyme linked immunosorbent detection
Ideal solution.
In correlation technique, the problem of APS-5 class enzyme-catalyzed chemical luminescence substrate existence and stability differences, it is impossible to steady in a long-term to protect
Deposit, the difficulty of its storage condition and use condition limits its application to a certain extent.
Therefore, it is necessary to providing a kind of new technology solves above-mentioned technical problem.
【The content of the invention】
The purpose of the present invention is to overcome above-mentioned technical problem, there is provided a kind of luminous signal is strong, very fast, lasting into plateau
Time is grown, the enzyme-catalyzed chemical luminescence substrate of normal temperature long shelf-life.
The technical scheme is that：
A kind of enzyme-catalyzed chemical luminescence substrate, the enzyme-catalyzed chemical luminescence substrate contain following component：
9- (4- chlorophenylthio benzoyls Oxymethylene) -10- methyl-acridan-disodium salt；
5- (myristoyl amido) fluorescein；
The acridine derivatives of aryl acylhydrazone -9,10.
Preferably, the enzyme-catalyzed chemical luminescence substrate is using Tris-Tween20 buffer solutions as solvent.
Preferably, the enzyme-catalyzed chemical luminescence substrate also contains ZnCl2、MgCl2, hexadecyltrimethylammonium chloride and anti-
Rotten agent.
Preferably, the enzyme-catalyzed chemical luminescence substrate includes following component：
Concentration is 0.2mol/L Tris buffer systems.
Preferably, the enzyme-catalyzed chemical luminescence substrate includes following component：
Concentration is 0.2mol/L Tris buffer systems.
Preferably, the preservative is Proclin-300.
Preferably, the structural formula of aryl acylhydrazone -9,10 acridine derivatives is as follows：
Wherein R is expressed as 9,10- acridines or 9,10- acridine derivatives.
Compared with correlation technique, enzyme-catalyzed chemical luminescence substrate provided by the invention, beneficial effect is：It is provided by the invention
Enzyme-catalyzed chemical luminescence substrate, by the formula of optimized emission substrate, increase aryl acylhydrazone -9,10 acridine derivatives work in formula
For luminescence enhancer, enhancing agent solution in substrate solution, can with alkaline phosphatase enzyme effect and light, its luminous intensity and alkali
Property connect smoked plum amount it is directly proportional, therefore the enzymatic luminous substrate have luminous signal it is strong, into plateau is very fast, the duration
The advantages that long；Simultaneously which raises the heat endurance of the enzyme-catalyzed chemical luminescence substrate and real-time stability, send out the enzymatic
Light substrate has the characteristics of normal temperature long shelf-life.
【Brief description of the drawings】
Fig. 1 is enzymatic luminous substrate Reaction kinetics research experimental result schematic diagram provided by the invention.
【Embodiment】
Below in conjunction with drawings and embodiments embodiment, the invention will be further described.
Embodiment one
The preparation of enzymatic luminous substrate
(1) material：5- (myristoyl amido) fluorescein, 9- (4- chlorophenylthio benzoyls Oxymethylene) -10- methyl -9,
10- acridans-disodium salt, MgCl2, ZnCl2, aryl acylhydrazone -9,10 acridine derivatives, hexadecyltrimethylammonium chloride
It is purchased in market, chemistry is pure；Proclin-300, Tween20, Tris are U.S. sigma-aldrich product.Wherein, aryl acyl
The acridine derivatives of hydrazone -9,10 are synthesized by aryl acylhydrazone with 9,10 acridine derivatives.
(2) using Tris-Tween20 buffer solutions as solvent, the enzyme-catalyzed chemical luminescence substrate of various concentrations is prepared, it is specific such as table
1.1- tables 1.4.
Table 1.1：Formula one
Table 1.2：Formula two
Table 1.3：Formula three
Table 1.4：Formula four
In enzyme-catalyzed chemical luminescence substrate provided by the invention, aryl acylhydrazone -9,10 acridine derivatives delay in chemiluminescence
Rush and play a part of fluorescence probe in system, make luminescence enhancement, when APS-5 runs into alkaline phosphatase, phosphate group can be sloughed,
Phosphate anion is generated, while occurs to light, aryl acylhydrazone -9,10 acridine derivatives in simultaneous buffering liquid can identify generation
Phosphate anion, fluorescent chelate can be formed, the transfer of generation energy between fluorescence be inspired, so that fluorescence signal is significantly
Tens times of enhancing, luminous signal is greatly improved to realize to fluorescence probe specific catalytic association reaction based on phosphate
Change.
Embodiment two
Compare the luminous of formula one, formula two, formula three, the enzyme-catalyzed chemical luminescence substrate for being formulated four and commercially available Lumigen
Substrate tests each gradient concentration AP signal intensities and linear relationship.Specific such as table 2.1- tables 2.5：
Table 2.1
Table 2.2
Table 2.3
Table 2.4
Table 2.5
Enzyme-catalyzed chemical luminescence substrate provided by the invention can be seen that by the detection data result of table 2.1- tables 2.5
Blank luminous intensity is not higher than 300；Under the conditions of same concentrations AP, the signal of enzyme-catalyzed chemical luminescence substrate provided by the invention is strong
The signal intensity of luminous substrate of the degree higher than commercially available Lumigen.
Embodiment three
Compare the luminous of formula one, formula two, formula three, the enzyme-catalyzed chemical luminescence substrate for being formulated four and commercially available Lumigen
Substrate determines the linear of carcinomebryonic antigen (CEA) immue quantitative detection reagent box.
CEA contents in human serum are detected using double antibody sandwich method, experimental procedure is as follows：
Step S1：Human serum sample or CEA calibration objects 30ul, the enzyme labelled antibody of dilution are separately added into chemical illuminating tube
100ul, coupled antibody magnetic bead 30ul, 37 DEG C of reaction 15min after mixing；
Step S2：300ul cleaning fluids are added to clean 3 times；
Step S3：Formula one, formula two, formula three, the enzymatic for being formulated four are separately added into different chemical illuminating tubes
Each 100ul of chemical luminous substrate and commercially available Lumigen chemical luminous substrate；
Step S4：Chemiluminescence Apparatus detects luminous intensity RLU values.
The testing result of luminous intensity RLU values such as table 3：
From the testing result of table 3, carcinomebryonic antigen (CEA) immue quantitative detection reagent box is measured under the same conditions
Linearly, the signal intensity of luminous substrate of the signal intensity of enzymatic luminous substrate provided by the invention higher than commercially available Lumigen.
Example IV
THERMAL STABILITY
To in embodiment one by formula one, formula two, formula three, the chemical luminous substrates that are prepared of formula four respectively at
Place to place for 10 days, 4 DEG C for 37 DEG C and carry out within 18 months heat endurance experiment and real-time stability experiment, experimental result such as table 4, table
Shown in 5：
Table 4：Heat endurance experimental result
Table 5：Real-time stability experimental result
From the signal retention rate data of table 4, table 5, enzyme-catalyzed chemical luminescence substrate heat endurance provided by the invention is real
Test and be all higher than 95% with the signal retention rate of real-time stability experiment, the results showed that enzyme-catalyzed chemical luminescence substrate of the invention can
It is steady in a long-term to preserve, can meet the needs of chemiluminescence instrument detection.
Embodiment five
Reaction kinetics research
The enzyme-catalyzed chemical luminescence substrate being prepared in comparative examples one by formula one, formula two, formula three, formula four
With commercially available Lumigen chemical luminous substrate and the luminous substrates of aryl acylhydrazone -9,10 acridine derivatives is not added with, is carried out anti-
Answer dynamics research.Referring to Fig. 1, illustrate for enzymatic luminous substrate Reaction kinetics research experimental result provided by the invention
Figure.Wherein, curve a represents commercially available Lumigen chemical luminous substrate polymerization kinetics curves figure；Curve b represents formula one
Enzyme-catalyzed chemical luminescence substrate polymerization kinetics curves figure；Curve c represents the enzyme-catalyzed chemical luminescence substrate kinetics of formula two
Curve map；Curve d represents the enzyme-catalyzed chemical luminescence substrate polymerization kinetics curves figure of formula three；Curve e represents the enzyme of formula four
Promote chemical luminous substrate polymerization kinetics curves figure；Curve f represents to be not added with the luminous bottom of the acridine derivatives of aryl acylhydrazone -9,10
Thing polymerization kinetics curves figure.
From accompanying drawing 1, enzyme-catalyzed chemical luminescence substrate provided by the invention can reach plateau (horizontal seat in accompanying drawing in 1min
Mark unit is the second), in enzyme-catalyzed chemical luminescence substrate provided by the invention, due to the addition of aryl acylhydrazone -9,10 acridine derivatives
As luminescence enhancer, hence it is evident that improve the chemiluminescence efficiency of APS-5 substrates.Formula one, formula two, it is formulated enzyme corresponding to four
It is suitable with the signal intensity of commercially available Lumigen chemical luminous substrate to promote the signal intensity of chemical luminous substrate, but is formulated three
Signal intensity of the signal intensity of corresponding enzyme-catalyzed chemical luminescence substrate apparently higher than commercially available Lumigen chemical luminous substrate.
Compared with correlation technique, enzyme-catalyzed chemical luminescence substrate provided by the invention, beneficial effect is：It is provided by the invention
Enzyme-catalyzed chemical luminescence substrate, by the formula of optimized emission substrate, increase aryl acylhydrazone -9,10 acridine derivatives work in formula
For reinforcing agent, enhancing agent solution in substrate solution, can with alkaline phosphatase enzyme effect and light, its luminous intensity and alkalescence company
The amount of smoked plum is directly proportional, thus the enzymatic luminous substrate have luminous signal it is strong, into plateau very fast, duration length etc.
Advantage；Simultaneously which raises the heat endurance of the enzyme-catalyzed chemical luminescence substrate and real-time stability, the enzymatic is set to light bottom
Thing has the characteristics of normal temperature long shelf-life.
Above-described is only embodiments of the present invention, it should be noted here that for one of ordinary skill in the art
For, without departing from the concept of the premise of the invention, improvement can also be made, but these belong to the protection model of the present invention
Enclose.
Claims (7)
1. a kind of enzyme-catalyzed chemical luminescence substrate, it is characterised in that the enzyme-catalyzed chemical luminescence substrate contains following component：
9- (4- chlorophenylthio benzoyls Oxymethylene) -10- methyl-acridan-disodium salt；
5- (myristoyl amido) fluorescein；
The acridine derivatives of aryl acylhydrazone -9,10.
2. enzyme-catalyzed chemical luminescence substrate according to claim 1, it is characterised in that the enzyme-catalyzed chemical luminescence substrate with
Tris-Tween20 buffer solutions are solvent.
3. enzyme-catalyzed chemical luminescence substrate according to claim 2, it is characterised in that the enzyme-catalyzed chemical luminescence substrate also contains
There is ZnCl2、MgCl2, hexadecyltrimethylammonium chloride and preservative.
4. enzyme-catalyzed chemical luminescence substrate according to claim 3, it is characterised in that the enzyme-catalyzed chemical luminescence substrate includes
Following component：
Concentration is 0.2mol/L Tris buffer systems.
5. enzyme-catalyzed chemical luminescence substrate according to claim 4, it is characterised in that the enzyme-catalyzed chemical luminescence substrate includes
Following component：
Concentration is 0.2mol/L Tris buffer systems.
6. enzyme-catalyzed chemical luminescence substrate according to claim 3, it is characterised in that the preservative is Proclin-300.
7. according to the enzyme-catalyzed chemical luminescence substrate any one of claim 1-6, it is characterised in that：The aryl acylhydrazone-
The structural formula of 9,10 acridine derivatives is as follows：
Wherein R is expressed as 9,10- acridines or 9,10- acridine derivatives.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108562735A (en) * | 2018-02-05 | 2018-09-21 | 江苏泽成生物技术有限公司 | A kind of enzyme-catalyzed chemical luminescence substrate liquid of alkaline phosphatase and preparation method thereof |
| CN109490281A (en) * | 2018-10-18 | 2019-03-19 | 深圳天深医疗器械有限公司 | Chemoluminescent substrate and its application |
| CN111077140A (en) * | 2020-01-07 | 2020-04-28 | 浙江拓创医疗科技有限公司 | Preparation method and filling device of enzymatic chemiluminescent substrate liquid |
| CN112067601A (en) * | 2020-08-05 | 2020-12-11 | 武汉生之源生物科技股份有限公司 | Alkaline phosphate enzymatic chemiluminescence substrate reinforcing agent and application thereof |
| CN112903664A (en) * | 2021-01-26 | 2021-06-04 | 金华市鑫科医疗器械有限公司 | Enzymatic chemiluminescence substrate liquid of alkaline phosphatase |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108562735A (en) * | 2018-02-05 | 2018-09-21 | 江苏泽成生物技术有限公司 | A kind of enzyme-catalyzed chemical luminescence substrate liquid of alkaline phosphatase and preparation method thereof |
| CN109490281A (en) * | 2018-10-18 | 2019-03-19 | 深圳天深医疗器械有限公司 | Chemoluminescent substrate and its application |
| CN111077140A (en) * | 2020-01-07 | 2020-04-28 | 浙江拓创医疗科技有限公司 | Preparation method and filling device of enzymatic chemiluminescent substrate liquid |
| CN112067601A (en) * | 2020-08-05 | 2020-12-11 | 武汉生之源生物科技股份有限公司 | Alkaline phosphate enzymatic chemiluminescence substrate reinforcing agent and application thereof |
| CN112067601B (en) * | 2020-08-05 | 2022-04-22 | 武汉生之源生物科技股份有限公司 | Alkaline phosphate enzymatic chemiluminescence substrate reinforcing agent and application thereof |
| CN112903664A (en) * | 2021-01-26 | 2021-06-04 | 金华市鑫科医疗器械有限公司 | Enzymatic chemiluminescence substrate liquid of alkaline phosphatase |
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