CN107447589A - A kind of preparation method of biology enzyme tin foil - Google Patents
A kind of preparation method of biology enzyme tin foil Download PDFInfo
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- CN107447589A CN107447589A CN201710847945.0A CN201710847945A CN107447589A CN 107447589 A CN107447589 A CN 107447589A CN 201710847945 A CN201710847945 A CN 201710847945A CN 107447589 A CN107447589 A CN 107447589A
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- Prior art keywords
- activated carbon
- tin foil
- biology enzyme
- preparation
- stirring
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H27/00—Special paper not otherwise provided for, e.g. made by multi-step processes
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
- D21H17/005—Microorganisms or enzymes
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Storage Of Fruits Or Vegetables (AREA)
Abstract
The present invention relates to a kind of preparation method of biology enzyme tin foil, belong to food preservative technology field.The present invention takes activated carbon ball milling to sieve, and obtains active carbon powder；Hydrochloric acid is poured into active carbon powder, washs, is poured into sodium hydroxide solution after agitation and filtration, agitator treating filtering, drying, obtains modified activated carbon；Peeling potatoes cleaning and chopping is taken, sodium dihydrogen phosphate disodium hydrogen phosphate buffer solution is poured into, ball milling, is placed in refrigerator, is then centrifuged for taking supernatant liquor；In mass ratio, activated carbon is poured into supernatant liquor, is placed in constant-temperature table concussion, is then stood in refrigerator, filter, be washed with deionized after washing filter paper with phosphate buffer solution, produce the carrier immobilized catalase of modified activated carbon；Retention agent, the carrier immobilized catalase of modified activated carbon and paper pulp are placed in mixer in mass ratio, slurries must be stirred by stirring；Stirring slurry is placed in copy and taken in bucket, adjusts pH, copies to take to be dried in vacuo after paper and produces a kind of biology enzyme tin foil.
Description
Technical field
The present invention relates to a kind of preparation method of biology enzyme tin foil, belong to food preservative technology field.
Background technology
China occupy world forefront in terms of the production of fruits and vegetables and in terms of consumption, and the fruits and vegetables amount consumed per capita every year is about
Reach 280kg, reach higher level.Because fresh fruit is after harvesting, environment changes, microbial infection and transported
The reason such as caused mechanical damage in journey, fruits and vegetables maturation speed can be all caused to be accelerated, freshness, nutritional ingredient and fruit
Flavor is all easy to decline, and therefore, the safely and effectively fresh-keeping problem direction for always being China's important research is carried out to fruits and vegetables.
Fruits and vegetables depart from after harvesting with parent, but still have vitality, and every physiological activity of fruits and vegetables is still normally being carried out,
Caused ethylene gas can accelerate the maturation of fruits and vegetables in storage, and respiration can consume the substantial amounts of organic matter of fruits and vegetables itself
Matter, so to reach the purpose for extending the preserving fruit and vegetable utilizing time, the most important respiratory rate for seeking to reduce fruits and vegetables.Currently used for
Reducing the preservation method of fruits and vegetables respiratory rate mainly has cryopreservation, controlled atmospheric packing storage, coating-film fresh-keeping and active packing to protect
It is fresh etc..In storage, gas constituent and ratio in environment have significant to the physiological change of fruits and vegetables after harvesting
Influence, oxygen content is than in the case of relatively low, can effectively suppress the respiration of fruits and vegetables and reduce the production of ethylene gas
Raw, ethene is as a kind of fruit-vegetable ripener, and accelerating aging to fruits and vegetables has large effect, so the generation of ethene suppressing is to extending
Preserving fruit and vegetable utilizing also plays the role of larger；At the same time, the low evaporation that can also mitigate fruits and vegetables of oxygen content and microorganism
Invade and harass, the carbon dioxide ratio in storage environment is properly increased also to be had to suppressing respiration, extending fruits and vegetables shelf life
Larger effect.
With the raising of people's living standard, consumption idea is also being constantly occurring change, not only to packaging for foodstuff
Outward appearance, aesthetic feeling propose higher requirement, and in food quality, nutrition and safety propose high requirement.It is raw
Thing enzyme preservation technology food preservative technology, is advocated as a kind of emerging effective and safe constantly by some experts, scholar.
Contribute to resources conservation, environmental protection using biological enzyme technology exploitation biology enzyme tin foil.Enterprises in pulp and paper industry is drawn
Enter the end-o f-pipe -control of biological enzyme technology, more than waste water,waste gas and industrial residue, also the more than improvement of some process, but
Pulping and paper-making production process is dissolved into natural ecosystems, at mashing in pulping and papermaking processes, filling, copy paper, waste water
The links such as reason all include the application category of biological enzyme technology, realize that reasonable efficiently utilization, the pollutant of raw material are minimum with this
Change, the purpose of changing waste into resources.
The content of the invention
The technical problems to be solved by the invention：For the cost consumption of existing preservation method great number, in developing country
Popularization is hardly resulted in, is not suitable for carrying out the problem of fresh-keeping to food with this method in transportation, there is provided a kind of biology enzyme
The preparation method of tin foil.
In order to solve the above technical problems, the technical solution adopted by the present invention is：
（1）Activated carbon is placed in ball milling in ball grinder, sieves, obtains active carbon powder；
（2）Hydrochloric acid is poured into active carbon powder, is washed with deionized after stirring, then pours into sodium hydroxide solution, after stirring
It is washed with deionized, is placed in baking oven and dries after filtering, obtain modified activated carbon；
（3）Fresh potato is taken, shreds, is placed in ball grinder after peeled wash, pours into sodium dihydrogen phosphate-disodium hydrogen phosphate buffering
Solution ball milling, it is placed in refrigerator after ball milling and stands, be subsequently placed at centrifuge, take supernatant liquor；
（4）In mass ratio 1：2, activated carbon is poured into supernatant liquor, is placed in constant-temperature table and shakes, mistake after being stood in refrigerator
Filter, is washed with sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution and deionized water, produces the carrier immobilized peroxide of modified activated carbon
Change hydrogen enzyme；
（5）In mass ratio 1：4：20, retention agent, the carrier immobilized catalase of modified activated carbon and paper pulp are placed in mixer
In, stirring slurry is obtained after stirring；
（6）Stirring slurry is placed in copy and taken in bucket, adds deionized water, is placed in vacuum desiccator and does after adjusting pH with hydrochloric acid solution
It is dry, produce a kind of biology enzyme tin foil.
Step（1）The mesh number of described sieving is 200.
Step（2）The quality of described hydrochloric acid is 2 times of quality of activated carbon, and its concentration is 0.1mol/L.
Step（2）The mass fraction of described sodium hydroxide is 8%.
Step（3）The pH of described sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution is 7.2.
Step（4）The temperature of described constant-temperature table is 2 ~ 4 DEG C.
Step（6）The pH of described regulation stirring slurry is 5 ~ 6.
Compared with other method, advantageous effects are the present invention：
（1）The present invention is during a kind of biology enzyme tin foil is prepared, the primary raw material using potato as enzyme processed, potato valency
Lattice are cheap, and source is wide, pollution-free；Preparation process of the present invention is easier, can be cost-effective.
（2）The present invention is that carrier fixes peroxidating with modified activated carbon during a kind of biology enzyme tin foil is prepared
Hydrogen enzyme, environmental protection, activated carbon can adsorb a part of ethene, and catalase has stronger antioxidation, be advantageous to eat
Product it is fresh-keeping.
Embodiment
Activated carbon is taken, is placed in ball grinder, with 300 ~ 400r/min rotating speed 30 ~ 40min of ball milling, 200 mesh sieves is crossed, obtains work
Property carbon powder；The 0.1mol/L of 2 times of active carbon powder quality hydrochloric acid is poured into active carbon powder, with 120 ~ 150r/min's
Rotating speed stirs 15 ~ 18h, is washed with deionized after filtering 5 ~ 6 times, pours into the sodium hydroxide solution that mass fraction is 8%, with
120 ~ 150r/min rotating speed stirs 3 ~ 4h, is washed with deionized 5 ~ 6 times, filters, is placed in 55 ~ 60 DEG C of baking oven and dries,
Produce modified activated carbon；Fresh potato is taken, removes the peel, is washed with deionized 2 ~ 3 times, is shredded, is placed in ball grinder, pours into
With potato quality identical pH be 7.2 sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution, with 300 ~ 400r/min rotating speed
50 ~ 60min of ball milling, place 1 ~ 2h in 0 ~ 4 DEG C of refrigerator, the ball milling slurry of freezing be placed in a centrifuge, 30000r/min from
20 ~ 30min of the heart, takes supernatant liquor；In mass ratio 1：2, activated carbon is poured into supernatant liquor, is placed in 2 ~ 4 DEG C of constant-temperature table
In 3 ~ 4h shaken with 120 ~ 130r/min rotating speed, with after standing 15 ~ 18h in 0 ~ 4 DEG C of refrigerator, filtering, use biphosphate
It is washed with deionized 5 ~ 6 times after sodium-disodium hydrogen phosphate buffer solution washing filter paper 2 ~ 3 times, produces modified active high-area carbon and consolidate
Surely catalase is changed；In mass ratio 1：4：20, retention agent, the carrier immobilized catalase of modified activated carbon and paper pulp are put
In mixer, 20 ~ 30min is stirred with 500 ~ 600r/min rotating speed, obtains stirring slurries；Stirring slurry is placed in copy and taken in bucket,
Add deionized water, be 5.0 ~ 6.0 with 1mol/L hydrochloric acid solution regulation pH, copy take be placed in after paper 100 in vacuum desiccator ~
105 DEG C of dry 5 ~ 8min, produce a kind of biology enzyme tin foil.Described retention agent is polyacrylamide.
Example 1
Activated carbon is taken, is placed in ball grinder, with 300r/min rotating speed ball milling 30min, 200 mesh sieves is crossed, obtains active carbon powder；
The 0.1mol/L of 2 times of active carbon powder quality hydrochloric acid is poured into active carbon powder, 15h, mistake are stirred with 120r/min rotating speed
It is washed with deionized after filter 5 times, pours into the sodium hydroxide solution that mass fraction is 8%, stirred with 120r/min rotating speed
3h, it is washed with deionized 5 times, filters, be placed in 55 DEG C of baking oven and dry, produce modified activated carbon；Fresh potato is taken,
Peeling, be washed with deionized 2 times, shred, be placed in ball grinder, pour into potato quality identical pH be 7.2 phosphoric acid
Sodium dihydrogen-disodium hydrogen phosphate buffer solution, with 300r/min rotating speed ball milling 50min, 1h in 0 DEG C of refrigerator is placed, will be freezed
Ball milling slurry be placed in a centrifuge, 30000r/min centrifuge 20 ~ 30min, take supernatant liquor；In mass ratio 1：2, by activated carbon
Pour into supernatant liquor, be placed in 2 DEG C of constant-temperature table and 3h is shaken with 120r/min rotating speed, with after quiet in 0 DEG C of refrigerator
15h is put, is filtered, is washed with deionized 5 times, produces after washing filter paper 2 times with sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution
The carrier immobilized catalase of modified activated carbon；In mass ratio 1：4：20, by retention agent, the carrier immobilized mistake of modified activated carbon
Hydrogen oxide enzyme and paper pulp are placed in mixer, are stirred 20min with 500r/min rotating speed, are obtained stirring slurries；Stirring slurry is placed in
Copy and take in bucket, add deionized water, be 5.0 with 1mol/L hydrochloric acid solution regulation pH, copy and be placed in vacuum desiccator after taking paper
In 100 DEG C of dry 5min, produce a kind of biology enzyme tin foil.
Example 2
Activated carbon is taken, is placed in ball grinder, with 350r/min rotating speed ball milling 35min, 200 mesh sieves is crossed, obtains active carbon powder；
The 0.1mol/L of 2 times of active carbon powder quality hydrochloric acid is poured into active carbon powder, 16h, mistake are stirred with 130r/min rotating speed
Be washed with deionized 5 times, poured into the sodium hydroxide solution that mass fraction is 8% after filter, with 130r/min rotating speed stirring 3 ~
4h, it is washed with deionized 5 times, filters, be placed in 58 DEG C of baking oven and dry, produce modified activated carbon；Fresh potato is taken,
Peeling, be washed with deionized 2 times, shred, be placed in ball grinder, pour into potato quality identical pH be 7.2 phosphoric acid
Sodium dihydrogen-disodium hydrogen phosphate buffer solution, with 350r/min rotating speed ball milling 55min, 1h in 2 DEG C of refrigerator is placed, will be freezed
Ball milling slurry be placed in a centrifuge, 30000r/min centrifugation 25min, take supernatant liquor；In mass ratio 1：2, activated carbon is fallen
Enter in supernatant liquor, be placed in 3 DEG C of constant-temperature table and 3h is shaken with 125r/min rotating speed, stood with after in 2 DEG C of refrigerator
16h, filtering, it is washed with deionized 5 times after washing filter paper 2 times with sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution, produces and change
Property absorbent charcoal carrier immobilized catalase；In mass ratio 1：4：20, by retention agent, the carrier immobilized peroxide of modified activated carbon
Change hydrogen enzyme and paper pulp is placed in mixer, 25min is stirred with 550r/min rotating speed, obtains stirring slurries；Stirring slurry is placed in and copied
Take in bucket, add deionized water, be 5.0 with 1mol/L hydrochloric acid solution regulation pH, copy and be placed in after taking paper in vacuum desiccator
102 DEG C of dry 6min, produce a kind of biology enzyme tin foil.
Example 3
Activated carbon is taken, is placed in ball grinder, with 400r/min rotating speed ball milling 40min, 200 mesh sieves is crossed, obtains active carbon powder；
The 0.1mol/L of 2 times of active carbon powder quality hydrochloric acid is poured into active carbon powder, 18h, mistake are stirred with 150r/min rotating speed
It is washed with deionized after filter 6 times, pours into the sodium hydroxide solution that mass fraction is 8%, stirred with 150r/min rotating speed
4h, it is washed with deionized 6 times, filters, be placed in 60 DEG C of baking oven and dry, produce modified activated carbon；Fresh potato is taken,
Peeling, be washed with deionized 3 times, shred, be placed in ball grinder, pour into potato quality identical pH be 7.2 phosphoric acid
Sodium dihydrogen-disodium hydrogen phosphate buffer solution, with 400r/min rotating speed ball milling 60min, 2h in 0 ~ 4 DEG C of refrigerator is placed, will be cold
The ball milling slurry of jelly is placed in a centrifuge, and 30000r/min centrifugation 30min, takes supernatant liquor；In mass ratio 1：2, by activated carbon
Pour into supernatant liquor, be placed in 4 DEG C of constant-temperature table and 4h is shaken with 130r/min rotating speed, with after quiet in 4 DEG C of refrigerator
18h is put, is filtered, is washed with deionized 6 times, produces after washing filter paper 3 times with sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution
The carrier immobilized catalase of modified activated carbon；In mass ratio 1：4：20, by retention agent, the carrier immobilized mistake of modified activated carbon
Hydrogen oxide enzyme and paper pulp are placed in mixer, are stirred 30min with 600r/min rotating speed, are obtained stirring slurries；Stirring slurry is placed in
Copy and take in bucket, add deionized water, be 6.0 with 1mol/L hydrochloric acid solution regulation pH, copy and be placed in vacuum desiccator after taking paper
In 105 DEG C of dry 8min, produce a kind of biology enzyme tin foil.
The Food Packaging Paper that biology enzyme tin foil made from this law and Guangdong Huizhou company produce is contrasted.
The fruit physiological change being housed in closed container, fresh-keeping, decayed fruit speed is carried out to fruit using biology enzyme tin foil
Degree is substantially slower than unused tin foil, and this immobilized catalase being primarily due in biology enzyme tin foil occurs anti-
Should, the oxygen in closed container is eliminated, makes oxygen content in the storage environment low, it is suppressed that the respiration of fruit, simultaneously
Caused hydrogen peroxide plays the role of sterilization during the course of the reaction, so as to inhibit the growth of microorganism；Directly use food bag
Fill paper and fruit carried out fresh-keeping, fruit begins to decayed fruit for second day, decayed fruit speed, due in closed environment respiration compared with
By force, substantial amounts of carbon dioxide and ethylene gas are produced, ethylene gas promotes the aging of fruit, tested after 6 days in two groups of experiments
The weight-loss ratio of fruit, as a result display are used biology enzyme tin foil and the weight-loss ratio point of fresh-keeping fruit are carried out using food wrapper
It is not 1.7% and 3.3%.With reference to these results suggest that biology enzyme tin foil has certain effect to the fresh-keeping of fruit, can reduce new
The decayed fruit rate of fresh fruit, so as to reach the purpose for extending fruit shelf life.Caused peroxidating during catalase reaction
Hydrogen has the function that sterilization, it is possible to reduce microorganism is infected to fruit.
Claims (7)
1. a kind of preparation method of biology enzyme tin foil, it is characterised in that specifically preparation process is：
Activated carbon is placed in ball milling in ball grinder, sieves, obtains active carbon powder；
Hydrochloric acid is poured into active carbon powder, is washed with deionized after stirring, then pours into sodium hydroxide solution, is used again after stirring
Deionized water is washed, and is placed in baking oven and is dried after filtering, obtains modified activated carbon；
Fresh potato is taken, shreds, is placed in ball grinder after peeled wash, it is molten to pour into sodium dihydrogen phosphate-disodium hydrogen phosphate buffering
Liquid ball milling, it is placed in refrigerator after ball milling and stands, be subsequently placed at centrifuge, take supernatant liquor；
In mass ratio 1:2, activated carbon is poured into supernatant liquor, is placed in constant-temperature table and shakes, is stood in subsequent refrigerator, mistake
Filter, is washed with sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution and deionized water, produces the carrier immobilized peroxide of modified activated carbon
Change hydrogen enzyme；
In mass ratio 1:4:20, retention agent, the carrier immobilized catalase of modified activated carbon and paper pulp are placed in mixer,
Stirring slurry is obtained after stirring；
Stirring slurry is placed in copy and taken in bucket, adds deionized water, is placed in vacuum desiccator and dries after adjusting pH with hydrochloric acid solution, i.e.,
Obtain a kind of biology enzyme tin foil.
A kind of 2. preparation method of biology enzyme tin foil according to claim 1, it is characterised in that：Step（1）Described
The mesh number of sieving is 200.
A kind of 3. preparation method of biology enzyme tin foil according to claim 1, it is characterised in that：Step（2）Described
The quality of hydrochloric acid is 2 times of quality of activated carbon, and its concentration is 0.1mol/L.
A kind of 4. preparation method of biology enzyme tin foil according to claim 1, it is characterised in that：Step（2）Described
The mass fraction of sodium hydroxide is 8%.
A kind of 5. preparation method of biology enzyme tin foil according to claim 1, it is characterised in that：Step（3）Described
The pH of phosphate buffer solution is 7.2.
A kind of 6. preparation method of biology enzyme tin foil according to claim 1, it is characterised in that：Step（4）Described
The temperature of constant-temperature table is 2 ~ 4 DEG C.
A kind of 7. preparation method of biology enzyme tin foil according to claim 1, it is characterised in that：Step（6）Described
The pH of stirring slurry is 5 ~ 6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710847945.0A CN107447589A (en) | 2017-09-19 | 2017-09-19 | A kind of preparation method of biology enzyme tin foil |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710847945.0A CN107447589A (en) | 2017-09-19 | 2017-09-19 | A kind of preparation method of biology enzyme tin foil |
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| CN201710847945.0A Pending CN107447589A (en) | 2017-09-19 | 2017-09-19 | A kind of preparation method of biology enzyme tin foil |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108477295A (en) * | 2018-04-09 | 2018-09-04 | 王景硕 | A kind of preparation method of fruits and vegetables fresh-keeping paper |
| CN108677611A (en) * | 2018-03-29 | 2018-10-19 | 句容市茂源织造厂 | A kind of activated carbon base tin foil and preparation method thereof |
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| CN103421762A (en) * | 2012-05-22 | 2013-12-04 | 北京化工大学 | Immobilized enzyme and preparation method thereof |
| WO2015074072A1 (en) * | 2013-11-18 | 2015-05-21 | Enzymatic Deinking Technologies, Llc | Enzymatic treatment of virgin fiber and recycled paper to reduce residual mineral oil levels for paper production |
| CN105780591A (en) * | 2016-03-11 | 2016-07-20 | 浙江金昌特种纸股份有限公司 | Method for making vegetable/fruit preservative paper |
| CN106320088A (en) * | 2016-08-18 | 2017-01-11 | 华南理工大学 | Fruit and vegetable preservative paper containing compound enzymes and preparation method thereof |
| CN107083723A (en) * | 2017-06-29 | 2017-08-22 | 苏州安特实业有限公司 | A kind of water repellent wrapping paper based on nanometer string and preparation method thereof |
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2017
- 2017-09-19 CN CN201710847945.0A patent/CN107447589A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103421762A (en) * | 2012-05-22 | 2013-12-04 | 北京化工大学 | Immobilized enzyme and preparation method thereof |
| WO2015074072A1 (en) * | 2013-11-18 | 2015-05-21 | Enzymatic Deinking Technologies, Llc | Enzymatic treatment of virgin fiber and recycled paper to reduce residual mineral oil levels for paper production |
| CN105780591A (en) * | 2016-03-11 | 2016-07-20 | 浙江金昌特种纸股份有限公司 | Method for making vegetable/fruit preservative paper |
| CN106320088A (en) * | 2016-08-18 | 2017-01-11 | 华南理工大学 | Fruit and vegetable preservative paper containing compound enzymes and preparation method thereof |
| CN107083723A (en) * | 2017-06-29 | 2017-08-22 | 苏州安特实业有限公司 | A kind of water repellent wrapping paper based on nanometer string and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108677611A (en) * | 2018-03-29 | 2018-10-19 | 句容市茂源织造厂 | A kind of activated carbon base tin foil and preparation method thereof |
| CN108477295A (en) * | 2018-04-09 | 2018-09-04 | 王景硕 | A kind of preparation method of fruits and vegetables fresh-keeping paper |
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