CN107446956A - A kind of method that arsenic hyperaccumulative plant ciliate desert-grass is handled using mesophilic anaerobic digestion - Google Patents

A kind of method that arsenic hyperaccumulative plant ciliate desert-grass is handled using mesophilic anaerobic digestion Download PDF

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Publication number
CN107446956A
CN107446956A CN201710685273.8A CN201710685273A CN107446956A CN 107446956 A CN107446956 A CN 107446956A CN 201710685273 A CN201710685273 A CN 201710685273A CN 107446956 A CN107446956 A CN 107446956A
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grass
ciliate desert
arsenic
anaerobic digestion
plant
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秦天悦
唐先进
刘杏梅
徐建明
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Zhejiang University ZJU
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Zhejiang University ZJU
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/023—Methane
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F11/00—Treatment of sludge; Devices therefor
    • C02F11/02—Biological treatment
    • C02F11/04—Anaerobic treatment; Production of methane by such processes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Water Supply & Treatment (AREA)
  • Environmental & Geological Engineering (AREA)
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  • Treatment Of Sludge (AREA)

Abstract

The invention discloses a kind of method that arsenic hyperaccumulative plant ciliate desert-grass is handled using mesophilic anaerobic digestion.This method obtains arsenic hyperaccumulative plant ciliate desert-grass first, and the stem and leaf that ciliate desert-grass is separated into and carries out broken segmentation；Then the ciliate desert-grass after will be broken is added in anaerobic digestion reaction vessel, is mixed with fermentate and is carried out mesophilic anaerobic digestion.Advantages of the present invention is mainly reflected in：(35 DEG C) pass through to ciliate desert-grass and organic solid castoff (sludge and animal waste etc.) progress mixing anaerobic digestion the inventive method under mesophilic condition, utilize existing a large amount of existing anaerobic digesters or marsh gas fermentation pool processing arsenic hyperaccumulative plant ciliate desert-grass, while organic solid castoff Anaerobic Digestion is promoted, eliminate rehabilitation plant secondary pollutions for caused by environment such as arsenic hyperaccumulative plant ciliate desert-grass, realize that arsenic hyperaccumulative plant harmlessness disposing utilizes, for As polluted soil phytoremediation further genralrlization using significant.

Description

A kind of method that arsenic hyperaccumulative plant ciliate desert-grass is handled using mesophilic anaerobic digestion
Technical field
It is more particularly to a kind of to utilize mesophilic anaerobic digestion the present invention relates to a kind of method of Anaerobic Digestion rehabilitation plant The method for handling arsenic hyperaccumulative plant ciliate desert-grass.
Background technology
It is current study hotspot both domestic and external using rehabilitation plant processing Heavy Metals in Soil Contaminated, but the production of rehabilitation plant After dispose, be in next step need discuss the problem of.It is existing at present burn, the reparation means such as landfill have that energy consumption is big, takes up an area Extensively, processing the deficiencies of causing secondary pollution to environment accidentally part, it is therefore necessary to develop energy-conserving and environment-protective, Labor-saving high-efficiency is repaiied Multiple plant method of disposal.
Anaerobic digestion is as a kind of conventional method for organic waste disposal, it is not necessary to consumes the substantial amounts of energy, moreover it is possible to produce Raw biogas, there are significant economic, ecology, environmental and social benefits, turn into and apply wider method at present.
Single plant digestion because carbon-nitrogen ratio, cellulignin content are high, cause degradation rate is low, anaerobic digestion time is long, Easily there is the problems such as floating layering.By feces of livestock and poultry and plant mixture slaking, anaerobic digestion process, increase vigor production can be accelerated Amount, had both solved rehabilitation plant and livestock waste process problem, alleviated the problem of rural area natural pond source shortage again, while also improve reparation Plant and the resource utilization rate of livestock waste.In addition, mixture slaking has many benefits, the nutrition as good in methane-generating pit is put down Weighing apparatus, and good fertilizer of quality etc..
Arsenic hyperaccumulative plant ciliate desert-grass is the hyperaccumulative plant that can largely adsorb heavy metals in soil arsenic, nowadays extensively The general As polluted soil that is applied to is handled, but the super tired plant arsenic containing high concentration in itself, it is impossible to for making feed, thus is faced Follow-up the problem of how handling.It is added in the present invention in excess sludge anaerobic digestion reaction vessel and carries out mixture slaking, Add the gas production and methane production of anaerobic digestion reaction.While organic solid castoff Anaerobic Digestion is promoted, Rehabilitation plant secondary pollutions for caused by environment such as arsenic hyperaccumulative plant ciliate desert-grass are eliminated, realize that arsenic hyperaccumulative plant is innoxious Treatment and use, for As polluted soil phytoremediation further genralrlization using significant.
Related patent has：The a kind of of Publication No. CN106119294A improves gramineae stalk anaerobic digestion gas production The preprocess method of efficiency, whole plant stalk is separated into the remainder of stem skin, stem marrow, blade and stalk, and using not Anaerobic digestion is carried out with processing mode.But it is the grasses such as common stalk that it, which handles target, does not relate to heavy metal Hyperaccumulative plant, the dual minimizing of anaerobic sludge digestion and heavy metal can not be realized.Publication No. 106591378A one kind The method that anaerobic digestion is carried out using enriching heavy metal ion biomass obsolete material, this method with heavy metal (including Cu, Zn, Co and Ni etc.) pollutant effluents is process object, it is that raw material passes through the biology that is prepared by garden garbage, agriculture and forestry organic waste material Matter charcoal or the plant for adsorbing heavy metal ion are launched into the waste water containing heavy metal ion, then by the material of acquisition Proportionally it is added in anaerobic digestion of kitchen wastes methane phase system, realizes the processing of biological material.But this method does not have Specifically for anionic heavy metal arsenic contaminated soil, and its digestion system fermentate used is kitchen garbage, not Using the existing anaerobic sludge digestion having a large capacity and a wide range or animal waste anaerobic fermentation system, applicability is narrower, generalization It is poor, it can not still be provided for how a large amount of ciliate desert-grass that As polluted soil phytoremediation is faced below further handle handling problems Effective solution.
The content of the invention
The object of the invention provides a kind of method that arsenic hyperaccumulative plant ciliate desert-grass is handled using mesophilic anaerobic digestion, promotes to have Machine solid waste anaerobic digestion process, gas production and methane production are improved, eliminate the reparations such as arsenic hyperaccumulative plant ciliate desert-grass and plant Thing secondary pollution for caused by environment, realize that arsenic hyperaccumulative plant harmlessness disposing utilizes.
In order to solve the above-mentioned technical problem, the present invention provides one kind using mesophilic anaerobic digestion processing arsenic hyperaccumulative plant Wu The method of centipede grass carries out anaerobic digestion, and the technical scheme of use is：
Using the method for mesophilic anaerobic digestion processing arsenic hyperaccumulative plant ciliate desert-grass, comprise the following steps：
Step 1：Arsenic hyperaccumulative plant ciliate desert-grass is obtained, and ciliate desert-grass is subjected to broken segmentation；
Step 2：Ciliate desert-grass after crushing is added in anaerobic digestion reaction vessel, and mixing progress anaeration in normal temperature with fermentate disappears Change.
Preferably, the arsenic hyperaccumulative plant ciliate desert-grass got need to be washed with deionized water only in advance, then naturally dry.
Preferably, the ciliate desert-grass after broken is 3~5cm per segment length.
Preferably, described ciliate desert-grass before excess sludge anaerobic digestion reaction vessel is added, carries out cauline leaf point in advance From then one kind in the whole plant of ciliate desert-grass, stem or leaf is individually added into reactor.Pass through different ciliate desert-grass positions Addition, can adjust the biogas production characteristics in anaerobic digestion process, including daily output tolerance, cumulative gas production and methane production.
Preferably, after ciliate desert-grass is added in reactor, in ciliate desert-grass and mud mixture containing arsenic concentration be 10~ 100mg·L-1。
Preferably, described fermentate is anaerobic sludge, animal waste or other organic solid castoffs.
Another object of the present invention is to provide a kind of utilize at arsenic hyperaccumulative plant ciliate desert-grass raising mesophilic anaerobic digestion The method for managing gas production, it is specially：The stem of arsenic hyperaccumulative plant ciliate desert-grass is individually added into excess sludge anaerobic digestion reaction vessel In, mixed with excess sludge and carry out mesophilic anaerobic digestion.The advantages of program, is after the stem of ciliate desert-grass is separated with blade, single Progress mesophilic anaerobic digestion is solely mixed with excess sludge can largely increase anaerobic digestion gas production amount
The beneficial effects are mainly as follows：Rehabilitation plant arsenic hyperaccumulative plant ciliate desert-grass is thrown according to a certain percentage It is put into anaerobic sludge digestion methane phase system, the organic loading of anaerobic digestion can be improved, increase methane production, is promoting to have While machine solid waste anaerobic digestion process, arsenic hyperaccumulative plant ciliate desert-grass is effectively handled, eliminates arsenic hyperaccumulative plant Wu The rehabilitation plants such as centipede grass secondary pollution for caused by environment, for the further genralrlization application tool of As polluted soil phytoremediation It is significant.
Brief description of the drawings
Fig. 1 is that the gas yield that different reactor is daily in Examples 1 and 2 changes over time curve map.
Fig. 2 is that different reactor cumulative gas output changes over time curve map in Examples 1 and 2.
Fig. 3 is that cumulative methane content changes over time curve map in different reactor generation gas in Examples 1 and 2.
Embodiment
With reference to two specific embodiments, the present invention is described further, makes the purpose of the present invention, technical scheme It is more clearly understood with advantage, the present invention is described in further detail, and result chart referring to the drawings is further proved.
It should be noted that the present invention digests to the mixing anaerobic of arsenic hyperaccumulative plant ciliate desert-grass and excess sludge, from logical Crossing the arsenic hyperaccumulative plant ciliate desert-grass of addition different proportion makes anaerobic digestion reaction vessel different containing arsenic concentration, and is surpassed by adding arsenic The different parts of accumulator plant ciliate desert-grass, including whole plant, stem and leaf (identical containing arsenic concentration in reactor), two aspects are commented The biogas production characteristics of the mixing anaerobic of valency arsenic hyperaccumulative plant ciliate desert-grass and excess sludge digestion.
Embodiment 1：
The arsenic hyperaccumulative plant ciliate desert-grass of collection divides whole plant, is washed with deionized water only, then naturally dry, by Wu Centipede grass Mechanical Crushing is into 3-5cm sections.It is determined by experiment, arsenic content is 3000mg/kg in whole plant.Disappear to different anaerobism Change the arsenic hyperaccumulative plant ciliate desert-grass whole plant that different proportion is put into reactor, make the mixed of ciliate desert-grass and sludge in reactor Compound is respectively 10mgL containing arsenic concentration-1, 50mgL-1, 100mgL-1(plant 10mgL is designated as respectively-1, plant 50mg·L-1, plant 100mgL-1).Mixing finishes, and different reactor under the conditions of water bath with thermostatic control heats 35 DEG C in carrying out Warm Anaerobic Digestion, should during collect caused gas in reactor, methane content in periodic monitoring aerogenesis, calculate accumulation Methane production.In the present embodiment, while the check experiment (being designated as CK) for not adding ciliate desert-grass is set, reaction condition and addition centipede The reactor of grass is identical.
It should be noted that arsenic hyperaccumulative plant ciliate desert-grass mixes with excess sludge and anaerobic digestion inoculation bacterium in the present embodiment Close uniform, input anaerobic digestion reaction vessel.But add reactor in sludge in itself contain anaerobic digestion flora, then without It need in addition be inoculated with, can realize that mesophilic anaerobic digestion is handled.
Compared with not adding arsenic hyperaccumulative plant ciliate desert-grass, addition arsenic hyperaccumulative plant ciliate desert-grass promotes anaerobic digestion Gas production and methane production in reactor.Wherein, adding arsenic concentration in arsenic hyperaccumulative plant ciliate desert-grass post-reactor is 100mg·L-1When anaerobic digestion reaction vessel gas production it is maximum (Fig. 1,2), 50mgL-1Reactor takes second place, 10mgL-1Reaction The increase of device gas production is less.However, (Fig. 3) is in opposite trend, 10mgL in terms of methane production-1Reactor production first Alkane amount is of a relatively high, 50mgL-1Reactor it is close but decrease, 100mgL-1Reactor accumulation methane phase Amount improves relatively fewer.
Arsenic hyperaccumulative plant ciliate desert-grass by adding different proportion makes anaerobic digestion reaction vessel different containing arsenic concentration, finds Thus caused different arsenic concentrations are different to the promotion degree of excess sludge anaerobic digestion.
Embodiment 2：
The arsenic hyperaccumulative plant ciliate desert-grass of collection divides whole plant, is washed with deionized water only, then naturally dry.Choose Part whole plant carries out cauline leaf separation, is divided into whole plant, stem and the class of leaf three, then carries out Mechanical Crushing respectively into 3-5cm Section.Because the arsenic content at each position of arsenic hyperaccumulative plant ciliate desert-grass is different, shows that arsenic ultraproduct is tired here by measuring and plant Thing ciliate desert-grass arsenic content is respectively：Whole plant 3000mg/kg, blade 3500mg/kg, stem 1500mg/kg.Respectively to different The one kind being individually added into excess sludge anaerobic digestion reaction vessel in whole plant, stem and the leaf of arsenic hyperaccumulative plant ciliate desert-grass, The mixture of ciliate desert-grass and sludge is set in reactor containing arsenic concentration to be 100mgL-1(plant 100mgL is designated as respectively-1, leaf 100mg·L-1, stem 100mgL-1).Mixing is finished, and 35 DEG C of progress mesophilic anaerobic digestion processing are heated in reactor water bath with thermostatic control, Should during collect caused gas in reactor, methane content in periodic monitoring aerogenesis, calculate cumulative methane yield.
Compared with whole plant is added to reactor, stem, which is individually put into excess sludge anaerobic digestion reaction vessel, to be increased Daily output tolerance at most (Fig. 1), secondly for individually addition blade.From the point of view of cumulative gas production (Fig. 2), individually adding stem can be very Increase anaerobic digestion gas production amount to big degree, whole plant is taken second place, and individually it is slightly inferior to compare the above two for addition blade.But in production first (Fig. 3) has been found that stem and leaf are individually added to anaerobic digestion in terms of alkane, relative to add whole plant and do not add plant (blank Control) methane production all declined, and whole plant is added to the production first that anaerobic digestion reaction vessel improves anaerobic digestion Alkane amount.
It should be noted that daily output tolerance and cumulative gas production are reacted in anaerobic digestion reaction vessel by measuring gas volume The running situation of anaerobic digestion reaction, accumulation methane production is then by reacting total methane production caused by whole period come direct table Show the using energy source situation in anaerobic digestion reaction vessel, reflect the high efficient resourcing degree of whole system.
By described above, implement the beneficial outcomes that the present invention is brought and be：
On the basis of existing rehabilitation plant and excess sludge recycling, by arsenic hyperaccumulative plant ciliate desert-grass according to one The different parts of arsenic hyperaccumulative plant ciliate desert-grass are added in excess sludge anaerobic digestion reaction vessel by certainty ratio, and raising is detested The organic loading of oxygen digestion, increases gas yield, while organic solid castoff Anaerobic Digestion is promoted, eliminates a huge sum of money Belong to super enriching plant secondary pollution for caused by environment, realize that arsenic hyperaccumulative plant harmlessness disposing utilizes, for arsenic pollution The further genralrlization of phytoremediation for soil is using significant.
It is pointed out that above-mentioned two embodiment carries out the place of ciliate desert-grass using excess sludge anaerobic digestion reaction vessel Reason, but actually can also be directly using existing a large amount of existing anaerobic digesters or marsh gas fermentation pool, fermentate is except adopting With the organic solid castoff such as animal waste can also be used outside excess sludge.
Disclosed above is only present pre-ferred embodiments, can not limit the right model of the present invention with this certainly Enclose, therefore the equivalent variations done according to the claims in the present invention, still belong to the scope that the present invention is covered.

Claims (7)

  1. A kind of 1. method that arsenic hyperaccumulative plant ciliate desert-grass is handled using mesophilic anaerobic digestion, it is characterised in that including following step Suddenly：
    Step 1：Arsenic hyperaccumulative plant ciliate desert-grass is obtained, and ciliate desert-grass is subjected to broken segmentation；
    Step 2：Ciliate desert-grass after will be broken is added in anaerobic digestion reaction vessel, is mixed with fermentate and is carried out mesophilic anaerobic digestion.
  2. 2. according to the method for claim 1, it is characterised in that the arsenic hyperaccumulative plant ciliate desert-grass got need to spend in advance Ionized water is cleaned, then naturally dry.
  3. 3. according to the method for claim 1, it is characterised in that the every segment length of ciliate desert-grass after broken is 3~5cm.
  4. 4. according to the method for claim 1, it is characterised in that described ciliate desert-grass is anti-in addition excess sludge anaerobic digestion Before answering device, cauline leaf separation is carried out in advance, one kind in the whole plant of ciliate desert-grass, stem or leaf is then individually added into reactor In.
  5. 5. according to the method for claim 1, it is characterised in that after ciliate desert-grass is added in reactor, ciliate desert-grass mixes with sludge Containing arsenic concentration it is 10~100mgL in compound-1。
  6. 6. according to the method for claim 1, it is characterised in that described fermentate be anaerobic sludge, animal waste or Other organic solid castoffs.
  7. A kind of 7. method that mesophilic anaerobic digestion processing gas production is improved using arsenic hyperaccumulative plant ciliate desert-grass, it is characterised in that The stem of arsenic hyperaccumulative plant ciliate desert-grass is individually added into excess sludge anaerobic digestion reaction vessel, mixed with excess sludge in carrying out Warm anaerobic digestion.
CN201710685273.8A 2017-08-11 2017-08-11 A kind of method that arsenic hyperaccumulative plant ciliate desert-grass is handled using mesophilic anaerobic digestion Pending CN107446956A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101492697A (en) * 2008-11-28 2009-07-29 四川大学 Enriched biological post-treatment technique for renovation of heavy metal polluted soil with combined mushroom-plant
CN102092913A (en) * 2010-11-24 2011-06-15 天津大学 Process for improving mesophilic anaerobic digestion effect by mixed fermentation of sludge and maize straws
WO2016077577A1 (en) * 2014-11-13 2016-05-19 Uchicago Argonne, Llc Method for generating methane from a carbonaceous feedstock

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101492697A (en) * 2008-11-28 2009-07-29 四川大学 Enriched biological post-treatment technique for renovation of heavy metal polluted soil with combined mushroom-plant
CN102092913A (en) * 2010-11-24 2011-06-15 天津大学 Process for improving mesophilic anaerobic digestion effect by mixed fermentation of sludge and maize straws
WO2016077577A1 (en) * 2014-11-13 2016-05-19 Uchicago Argonne, Llc Method for generating methane from a carbonaceous feedstock

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
宁平等: "《西南地区砷富集植物筛选及应用》", 31 October 2012, 冶金工业出版社 *

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