CN107446918B - Method for obtaining MYC gene in human tissue by two-step overlapping PCR method - Google Patents
Method for obtaining MYC gene in human tissue by two-step overlapping PCR method Download PDFInfo
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- CN107446918B CN107446918B CN201710708098.XA CN201710708098A CN107446918B CN 107446918 B CN107446918 B CN 107446918B CN 201710708098 A CN201710708098 A CN 201710708098A CN 107446918 B CN107446918 B CN 107446918B
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- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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CN201710708098.XA CN107446918B (en) | 2017-08-17 | 2017-08-17 | Method for obtaining MYC gene in human tissue by two-step overlapping PCR method |
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CN201710708098.XA CN107446918B (en) | 2017-08-17 | 2017-08-17 | Method for obtaining MYC gene in human tissue by two-step overlapping PCR method |
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CN107446918A CN107446918A (en) | 2017-12-08 |
CN107446918B true CN107446918B (en) | 2021-04-13 |
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102191336A (en) * | 2011-06-09 | 2011-09-21 | 广州益善生物技术有限公司 | MYC gene single nucleotide polymorphism (SNP) detection specific primer and liquid-phase chip |
CN103764849A (en) * | 2011-06-27 | 2014-04-30 | 佛罗里达大学研究基金公司 | Method for genome complexity reduction and polymorphism detection |
CN105238867A (en) * | 2015-11-05 | 2016-01-13 | 复旦大学 | Methane/ ammoxidation bacterial community structure analysis method based on two-step method |
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2017
- 2017-08-17 CN CN201710708098.XA patent/CN107446918B/en active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102191336A (en) * | 2011-06-09 | 2011-09-21 | 广州益善生物技术有限公司 | MYC gene single nucleotide polymorphism (SNP) detection specific primer and liquid-phase chip |
CN103764849A (en) * | 2011-06-27 | 2014-04-30 | 佛罗里达大学研究基金公司 | Method for genome complexity reduction and polymorphism detection |
CN105238867A (en) * | 2015-11-05 | 2016-01-13 | 复旦大学 | Methane/ ammoxidation bacterial community structure analysis method based on two-step method |
Non-Patent Citations (3)
Title |
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splicing by overlap extension PCR to obtain hybrid DNA products;Thornton, Justin A;《Methods in molecular biology》;20161231;摘要 * |
胃癌C-MYC癌基因扩增的临床意义;黄焰等;《军事医学科学院院刊》;19941231;第18卷(第1期);第46-47页 * |
重叠延伸PCR技术及其在基因工程上的应用;徐芳等;《分子植物育种》;20061231;第4卷(第5期);第747-750页 * |
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Inventor after: Hua Ziang Inventor after: Ma Hui Inventor after: Wan Junxing Inventor after: Zhou Xiang Inventor after: Ma Hu Inventor after: Zhang Minghe Inventor after: Zhang Wei Inventor before: Ma Hui Inventor before: Wan Junxing Inventor before: Zhou Xiang Inventor before: Ma Hu Inventor before: Zhang Minghe Inventor before: Zhang Wei |
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