CN107441148A - A kind of serum grass extractive of general flavone and preparation method thereof and antitumor activity application - Google Patents
A kind of serum grass extractive of general flavone and preparation method thereof and antitumor activity application Download PDFInfo
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- CN107441148A CN107441148A CN201710704150.4A CN201710704150A CN107441148A CN 107441148 A CN107441148 A CN 107441148A CN 201710704150 A CN201710704150 A CN 201710704150A CN 107441148 A CN107441148 A CN 107441148A
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/47—Euphorbiaceae (Spurge family), e.g. Ricinus (castorbean)
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
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Abstract
The present invention relates to a kind of serum grass extractive of general flavone and preparation method thereof and antitumor activity application, belong to pharmaceutical technology field, using it as antitumor made from raw material and antitumor auxiliary new drug, can effectively suppress tumour growth, reach the purposes for the treatment of tumour.The beneficial effect of the invention is to have filled up the blank for preparing serum grass extractive of general flavone and extended it preparing the application in antineoplastic and antitumour auxiliary drug field, so as to improve competitiveness of such preparation in international market.
Description
Technical field
The invention belongs to pharmaceutical technology field, is related to serum grass extractive of general flavone and preparation method thereof, using it as raw material
The application of obtained antitumor and antitumor auxiliary new drug, can effectively suppress tumour growth, reach the purposes for the treatment of tumour.
Background technology
Cancer is a kind of common disease and frequently-occurring disease of serious threat human life and health, is worldwide refractory disease, many
National tumor mortality rate occupies the first place of the various causes of the death.Clinically classic chemotherapy medicine belongs to cytotoxic drug, cell toxicity medicament
While tumour cell is killed, human normal cell is also destroyed, selectivity is low, toxicity is big for it, and long-term prescription can be brought sternly
Weight late morbidity is the generation of secondary tumour, especially causes acute nonlymphocytic leukemia and myeloproliferative disorder comprehensive
The serious toxic side effect such as simulator sickness.Modern medical research is proved, a very big change for treating tumour is contained in Chinese herbal medicine
Compound storehouse, chemotherapeutics many at present is directed to the purifying of these Chinese herbal medicine components, or is transformed using it as precursor,
Make potent, Small side effects antineoplastics.Active component Chinese medicine (kind new medicine of Chinese medicine five) due to can embody more than Chinese medicine into
Point, Mutiple Targets, multipath play drug effect the characteristics of, and can makes effective ingredient more be enriched with, pharmacological action and clinical efficacy
Enhancing, and beneficial to advantages such as quality controls, in recent years as one of the important directions of Chinese medicine, crude drug new drug development.
The dry aerial parts that serum grass is euphorbia plant Euphorbia esula L Euphorbia esula L., are distributed in the whole nation
(in addition to Hainan, Guizhou, Yunnan and Tibet), roadside, weeds clump, hillside, sylvan life, stream side, deserted mountain, sand dune and meadow are born in,
With draw out pus by applying a plaster to the affected part, dissipating bind, defaecation, diuresis and other effects, it is among the people to be used to treat nameless sores or boils, bone tuberculosis, scrofula, oedema, ox
Psoriasis and scabies etc., it is the legal local medicinal material in Jilin Province, and primary standard is recorded in Jilin Province's drug standards version in 1977.Have at present
The document report closed in terms of the medicinal ingredient analysis and research is few, has scholar to isolate 3 diterpene chemical combination from Euphorbia esula L
Thing[1]；Isolated Quercetin -3-O- the alpha-L-rhamnosides of Euphorbia esula L ethyl acetate extract, Kaempferol -3-O- β-D- pyrroles
Glucopyranoside glycosides, Quercetin -7-O- β-D- 3 flavones monomeric compounds of glucopyranoside[2].In terms of pharmacological research, relevant breast
Refer to that Euphorbia esula L alcohol extract can raise bax and downward by mitochondria pathway in slurry root of Beijing euphorbia extracorporeal anti-tumor cell research report
Bcl-2 expression, human lung cancer cell A549's apoptosis is induced, so as to suppress the propagation of A549 cells, plays effect of anti-lung cancer[3][4]；
Euphorbia esula L alcohol extract makes HeLa thin by lowering Cyclin D1, Cyclin E and CDK4 expression and raising P21 expression
Born of the same parents are arrested in G0/G1Phase, and then Cervical Cancer HeLa Cells growth and propagation are significantly inhibited, inducing cell relies on the mitochondrial pathways
Apoptotic death[5].And the research and its antitumor experimental study in vivo to its extractive of general flavone preparation method still belong to blank,
The present invention will fill up above-mentioned blank, and the comprehensive development and utilization for serum herbal medicine material provides broader thinking.
The content of the invention
It is an object of the invention to provide a kind of serum grass extractive of general flavone and preparation method thereof and antitumor activity application,
Also provide using it as antitumor made from raw material and antitumor auxiliary new drug, can effectively suppress tumour growth, reach treatment
The purposes of tumour.
Serum grass extractive of general flavone is to extract to obtain from the dry aerial parts of euphorbia plant Euphorbia esula L, always
Flavones content is 20%~100%.
A kind of serum grass extractive of general flavone provided by the invention and preparation method thereof is preferred, comprises the following steps：
(1) extract：Take serum careless (Euphorbia esula L ground drying nest), give as one thinks fit cataclasm, add water to cook, control adds water
Amount, extraction time and extraction time.
(2) separate：Merge the extract solution under (1) item, filter while hot, filtrate concentration, the concentrate is added on and located in advance
In the large pore resin absorption column managed, first with the washing of 4~12 times of resin column volumes, then with 4~12 times of posts fill volume, concentration are
30~95% ethanol elution, eluent is collected, be concentrated and dried after reclaiming ethanol, obtain serum grass general flavone crude extract.
(3) purify：With concentration be 30~95% ethanol as solvent, by crude extract add 4~12 times of weight the solvent
Middle stirring 10~40 minutes, filtration, filtrate water-bath is concentrated into thick paste, is dried under reduced pressure, and serum grass extractive of general flavone is made.
2nd, preparation method as claimed in claim 1, it is characterised in that step (1) water that adds is the 6 of medicinal material amount
~12 times；Extraction time is 1~3 hour；Extraction time is 1~3 time.
3rd, preparation method as claimed in claim 1, it is characterised in that macroporous absorbent resin described in step (2) is D101、
AB-8、D21Or flavones resin.
4th, serum grass extractive of general flavone as claimed in claim 1 is preparing antineoplastic and antitumour auxiliary drug
In application.
The serum grass extractive of general flavone and pharmaceutically acceptable carrier or figuration of the present invention also offer present invention
New drug preparation prepared by agent.These preparations select following formulations：Solution, suspension, emulsion, tablet, capsule, granule,
Pill, lozenge oral liquid, sublingual tablet, effervescent tablet, injection etc..In these preparations, except including extractive of general flavone of the present invention, also
Sustained release or controlled release preparation can be prepared into as needed.
Pharmaceutical preparation of the invention containing serum grass extractive of general flavone, can also optionally contain when preparing pharmaceutical preparation
Pharmaceutically acceptable carrier, carrier come from：Cosolvent, solubilizer, suspending agent, emulsifying agent, surfactant, flavouring etc..
It is provided by the invention not only to include single preparations of ephedrine by new drug made from raw material of serum grass extractive of general flavone, also wrap
Include and medicine of the serum grass extractive of general flavone as active component is added in compound preparation.
It is provided by the invention using serum grass extractive of general flavone its as antitumor made from raw material and antitumor auxiliary new drug,
Tumour growth can effectively be suppressed, reach the purposes for the treatment of tumour.
The beneficial effects of the invention are as follows：Fill up the blank for preparing serum grass extractive of general flavone and extend it anti-swollen
Knurl field of medicaments application, so as to improve competitiveness of such Chinese medicine preparation in international market.
Embodiment
It is that the present invention is further described in more detail below by way of specific embodiment, but not to the scope of the present invention structure
Into any restrictions.
Experimental example serum grass extractive of general flavone preparation method
Embodiment 1：
(1) serum herbal medicine material (Euphorbia esula L ground drying nest) 3kg is taken, gives as one thinks fit cataclasm, adds 24kg water boiling and extractions 2
It is secondary, 2 hours every time.
(2) merging filtrate, filter while hot, it is 1L to be concentrated into volume, is added on processed good D101Macroporous resin column, post bed
Volume 1L, first with 8L water elutions, then with the ethanol elutions of 8L 95%, it is concentrated and dried after eluent recovery ethanol, it is total yellow obtains serum grass
Ketone crude extract 117g.
(3) crude extract is taken, stirs 30 clocks, filtration with 40% ethanol 936ml, filtrate water-bath is concentrated into thick paste, and decompression is dry
It is dry, serum grass extractive of general flavone 73g is made.Obtained serum grass general flavone content is 86%.
Embodiment 2：
(1) serum herbal medicine material (Euphorbia esula L ground drying nest) 3kg is taken, gives as one thinks fit cataclasm, adds 18kg water boiling and extractions 3
It is secondary, 1 hour every time.
(2) merging filtrate, filter while hot, it is 1L to be concentrated into volume, is added on processed good AB-8 macroporous resin columns, post bed
Volume 1L, first with 6L water elutions, then with the ethanol elutions of 6L 30%, it is concentrated and dried after eluent recovery ethanol, it is total yellow obtains serum grass
Ketone crude extract 127g.
(3) crude extract is taken, stirs 20 clocks, filtration with 75% ethanol 762ml, filtrate water-bath is concentrated into thick paste, and decompression is dry
It is dry, serum grass extractive of general flavone 76g is made.Obtained serum grass general flavone content is 52%.
Embodiment 3：
(1) serum herbal medicine material (Euphorbia esula L ground drying nest) 3kg is taken, gives as one thinks fit cataclasm, adds 27kg water boiling and extractions 2
It is secondary, 1.5 hours every time.
(2) merging filtrate, filter while hot, it is 1L to be concentrated into volume, is added on processed good D21Macroporous resin column, post bed body
Product 1L, first with 10L water elutions, then with the ethanol elutions of 10L 70%, it is concentrated and dried after eluent recovery ethanol, it is total yellow obtains serum grass
Ketone crude extract 144g.
(3) crude extract is taken, is stirred 10 minutes with 50% ethanol 1440ml, filtration, filtrate water-bath is concentrated into thick paste, depressurizes
Dry, serum grass extractive of general flavone 52g is made.Obtained serum grass general flavone content is 67%.
Embodiment 4：
(1) serum herbal medicine material (Euphorbia esula L ground drying nest) 3kg is taken, gives as one thinks fit cataclasm, adds 30kg water boiling and extractions 1
It is secondary, 3 hours.
(2) merging filtrate, filter while hot, it is 1L to be concentrated into volume, is added on processed good flavones resin column, bed volume
1L, first with 5L water elutions, then with the ethanol elutions of 5L 50%, it is concentrated and dried after eluent recovery ethanol, it is thick obtains serum grass general flavone
Extract 108g.
(3) crude extract is taken, is stirred 15 minutes with 95% ethanol 540ml, filtration, filtrate water-bath is concentrated into thick paste, depressurizes
Dry, serum grass extractive of general flavone 71g is made.Obtained serum grass general flavone content is 28%.
Experimental example
(1) serum grass extractive of general flavone is to S180The inhibitory action of tumor-bearing mice
Medicine of the present invention is proved to S through pharmacodynamic experiment180Tumor-bearing mice has an obvious inhibitory action, and 100.0,50.0mg/
Kg tested materials are to murine sarcoma (S180) there is obvious inhibitory action.
1. material
1.1 animal Kunming mouses, male and female half and half, 18~22g of body weight, purchased from Changchun hundred million, this experimental animal technology is limited
Responsible company, SPF levels, production unit animal quality credit number：SCXK- (Ji) 2011-0004, Jilin Province's Quality of Experimental Animals
Quality certification sequence number：201600015498.Experimental animal breeds mouse pellet, raw material composition：Corn, fish meal, dregs of beans, bicarbonate
Calcium, flour, stone flour etc. form, manufacturer：This experimental animal technology Co., Ltd of Changchun hundred million, production licence：SCXK-
(Ji) 2003-0008, date of manufacture：20161201.The KFL-400 that mouse drinking-water is produced using Kai Fulong Science and Technology Ltd.s is pure
Pure water prepared by water dispenser.
1.2 experimental enviroment mouse are raised in laboratory animal room of Jilin Academy of Chinese Medicine Sciences, barrier system, experimental animal
Use credit number：SYXK (Ji) 2015-0009.Animal room temperature：19~23 DEG C, relative humidity 45~65%, illumination：12 is small
Shi Ming, 12 hours dark.
1.3 medicine serum grass extractive of general flavone, yellow, provided by Jilin Academy of Chinese Medicine Sciences's new drug center, content
Prepared for 82%, during experiment with 0.5% sodium carboxymethylcellulose.Endoxan is given birth to for Hengrui Medicine Co., Ltd., Jiangsu Prov.
Production, batch number 16032625.
1.4 knurl strain mouse S180Knurl strain is purchased from treatment and prevention of tumour research institute of tumour hospital of Jilin Province.
1.5 instrument JA2003B one thousandth balances, Shanghai Yue Ping scientific instrument Co., Ltd product.JJ200 precise electronics
Balance, Y-2000 type electronic balances, it is the manufacture of Changshu Shuan Jie testers factory.S70-CD-1FD superclean benches and BXM-
30R vertical pressure steam sterilizers, it is Shanghai Medical Equipment Plant of Bo Xun Industrial Co., Ltd.s product.
2. experimental method and result
Take Kunming kind female mice 50, every group 10, every mouse Iodophors and alcohol disinfecting skin, in right fore
Subcutaneous vaccination S180(oncocyte number is 2 × 10 by cytoma liquid 0.2ml6~2.2 × 106In the range of), after 24 hours, random packet.
Experiment sets the sodium carboxymethylcelluloses of control group gavage 10ml/kg 0.5%, intraperitoneal injection of cyclophosphamide 20mg/ the next day positive drug group
Kg, the daily gavage serum grass extractive of general flavone 100.0mg/kg of No. 1 medicine group, the daily gavage serum grass general flavone of No. 2 medicine groups carry
Thing 50.0mg/kg, the daily gavage serum grass extractive of general flavone 25.0mg/kg of No. 3 medicine groups are taken, once a day, continuous 10 days, end
Put to death mouse within 1 hour after secondary administration, weigh, stripping tumor mass is weighed, and calculates tumour inhibiting rate.It the results are shown in Table 1.
The serum grass extractive of general flavone of table 1 is to S180The inhibitory action of sarcoma
As a result show, cyclophosphamide Injection is to S180Growing sarcoma significantly inhibits effect, inhibiting rate 56.74%.Breast
Careless extractive of general flavone 100.0,50.0,25.0mg/kg gastric infusions are starched to S180The inhibiting rate of murine sarcoma is respectively 43.89,
32.54th, 19.01%, wherein 100.0,50.0mg/kg groups there were significant differences compared with control group (P<0.001 and P<0.01) it is, right
S180The growth of murine sarcoma has obvious inhibitory action.
(2) serum grass extractive of general flavone is to rat liver cancer H22The inhibitory action of solid tumor
Medicine of the present invention is proved to rat liver cancer H through pharmacodynamic experiment22The obvious inhibitory action of solid tumor, 100.0,
50.0th, 25.0mg/kg tested materials are to rat liver cancer (H22) there is obvious inhibitory action.
1.1 animal Kunming mouses, male and female half and half, 18~22g of body weight, purchased from Changchun hundred million, this experimental animal technology has
Limit responsible company, SPF levels, production unit animal quality credit number：SCXK- (Ji) 2011-0004, Jilin Province's experimental animal matter
Measure quality certification sequence number：201600015498.Experimental animal breeds mouse pellet, raw material composition：Corn, fish meal, dregs of beans, carbonic acid
Hydrogen calcium, flour, stone flour etc. form, manufacturer：This experimental animal technology Co., Ltd of Changchun hundred million, production licence：
SCXK- (Ji) 2003-0008, date of manufacture：20161201.The KFL- that mouse drinking-water is produced using Kai Fulong Science and Technology Ltd.s
Pure water prepared by 400 water purification machines.
1.2 experimental enviroment mouse are raised in laboratory animal room of Jilin Academy of Chinese Medicine Sciences, barrier system, and experiment is dynamic
Thing uses credit number：SYXK (Ji) 2015-0009.Animal room temperature：19~23 DEG C, relative humidity 45~65%, illumination：12
Hour is bright, and 12 hours dark.
1.3 medicine serum grass extractive of general flavone, yellow, provided by Jilin Academy of Chinese Medicine Sciences's new drug center, content
Prepared for 82%, during experiment with 0.5% sodium carboxymethylcellulose.Endoxan is given birth to for Hengrui Medicine Co., Ltd., Jiangsu Prov.
Production, batch number 16032625.
1.4 knurl strain mouse H22Knurl strain is purchased from treatment and prevention of tumour research institute of tumour hospital of Jilin Province.
1.5 instrument JA2003B one thousandth balances, Shanghai Yue Ping scientific instrument Co., Ltd product.The accurate electricity of JJ200
Sub- balance, Y-2000 type electronic balances, it is the manufacture of Changshu Shuan Jie testers factory.S70-CD-1FD superclean benches and BXM-
30R vertical pressure steam sterilizers, it is Shanghai Medical Equipment Plant of Bo Xun Industrial Co., Ltd.s product.
2. experimental method and result
Take ICR male mices 50, every group 10, every mouse Iodophors and alcohol disinfecting skin, in right fore skin
Lower inoculation H22(oncocyte number is 2 × 10 by cytoma liquid 0.2ml6~2.2 × 106In the range of), after 24 hours, random packet.It is real
Test and set the sodium carboxymethylcelluloses of control group gavage 10ml/kg 0.5%, intraperitoneal injection of cyclophosphamide 20mg/ the next day positive drug group
Kg, the daily gavage serum grass extractive of general flavone 100.0mg/kg of No. 1 medicine group, the daily gavage serum grass general flavone of No. 2 medicine groups carry
Thing 50.0mg/kg, the daily gavage serum grass extractive of general flavone 25.0mg/kg of No. 3 medicine groups are taken, once a day, continuous 10 days, end
Put to death mouse within 1 hour after secondary administration, weigh, stripping tumor mass is weighed, and calculates tumour inhibiting rate.It the results are shown in Table 2.
The serum grass extractive of general flavone of table 2 is to mouse H22The influence of solid tumor
Test result indicates that cyclophosphamide Injection is to H22The growth of liver-cancer solid tumor significantly inhibits effect, inhibiting rate
For 58.47%.Serum grass extractive of general flavone 100.0,50.0,25.0mg/kg gastric infusions are to H22The inhibiting rate of murine sarcoma
Respectively 43.68,32.22,22.58%.100.0th, 50.0,25.0mg/kg groups there were significant differences compared with control group (P<
0.001, P<0.01, P<0.05), to H22The growth of rat liver cancer solid tumor has significant inhibitory action.
Bibliography
1Wang YB, Ji P, Wang HB, Qin GW.Diterpenoids from Euphorbia esula [J]
.Chinese Journal of Natural Medicines, 2010,8 (2)：94-96.
2 bavin states give birth to, flavonoids research [J] Qiqihar Universities report in Zhao Ming Euphorbia esula Ls, and 2012,25
(2)：9-10.
3 Yang Wen are outstanding, flourishing to clouds, Cheng Jianhui, Zhang Xianghong, horse, and king likes red Euphorbia esula Ls extract to human lung carcinoma cell
Influence [J] the Shandong medicine of A549 propagation, 2014,54 (35)：13-15.
4 kings like red, Pang Qiuxia, Chen Meixia, meter Zhi Kuan, Fu Zhaoying, Yang Wenjie, to clouds Euphorbia esula L extracts to people
Influence [J] the Mountain Western Medicine S University journal of lung cancer cell growth, 2014,45 (6)：460-464.
5 bright flat red, Chen Hongbo, Cai Ting, Huang Laiqiang, Zhou Lanzhen, Guo Caiping, peaceful brave Euphorbia esula Ls extract induction Hela
Apoptosis and its treasure's traditional Chinese medical science traditional Chinese medicines during Mechanism Study [J], 2013,24 (6)：1318-1321.
Claims (4)
- A kind of 1. serum grass extractive of general flavone, it is characterised in that：Extractive of general flavone is from euphorbia plant Euphorbia esula L Extraction obtains in the dry aerial parts of (Euphorbia esula L.), and general flavone content is 20%~100%；Obtained by following methods：(1) extract：Take serum careless (Euphorbia esula L ground drying nest), give as one thinks fit cataclasm, add water to cook, control adds water, carried Take time and extraction time；(2) separate：Merge the extract solution under (1) item, filter while hot, filtrate concentration, the concentrate is added on and anticipated Large pore resin absorption column in, first with the washing of 4~12 times of resin column volumes, then with 4~12 times of posts fill volumes, concentration be 30~ 95% ethanol elution, eluent is collected, be concentrated and dried after reclaiming ethanol, obtain serum grass general flavone crude extract；(3) purify：With the ethanol as solvent that concentration is 30~95%, crude extract is added in the solvent of 4~12 times of weight and stirred Mix 10~40 minutes, filter, filtrate water-bath is concentrated into thick paste, is dried under reduced pressure, and serum grass extractive of general flavone is made.
- 2. preparation method as claimed in claim 1, it is characterised in that step (1) water that adds is the 6~12 of medicinal material amount Times；Extraction time is 1~3 hour；Extraction time is 1~3 time.
- 3. preparation method as claimed in claim 1, it is characterised in that macroporous absorbent resin described in step (2) is D101、AB- 8、D21Or flavones resin.
- 4. serum grass extractive of general flavone as claimed in claim 1 is in antineoplastic and antitumour auxiliary drug is prepared Using.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1733752A (en) * | 2005-08-22 | 2006-02-15 | 广西中医学院 | Emblic leafflower fruit extract possessing anticancer, antibiotic actions and its manufacturing method of traditional Chinese medicine formulation |
| CN103655674A (en) * | 2012-09-11 | 2014-03-26 | 清华大学深圳研究生院 | Euphorbia esula extract as well as preparation method and application thereof |
-
2017
- 2017-08-17 CN CN201710704150.4A patent/CN107441148A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1733752A (en) * | 2005-08-22 | 2006-02-15 | 广西中医学院 | Emblic leafflower fruit extract possessing anticancer, antibiotic actions and its manufacturing method of traditional Chinese medicine formulation |
| CN103655674A (en) * | 2012-09-11 | 2014-03-26 | 清华大学深圳研究生院 | Euphorbia esula extract as well as preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 王爱红，等: "乳浆大戟提取物对人肺癌细胞生长的影响", 《山西医科大学学报》 * |
| 田瑛，等: "大戟科黄酮类化合物的研究进展", 《国际药学研究杂志》 * |
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