CN107439548B - Bactericidal composition containing orysastrobin and shenqinmycin - Google Patents

Abstract

The invention provides a sterilization composition containing orysastrobin and shenqinmycin, wherein the weight ratio of orysastrobin to shenqinmycin is 1:80-80:1, and preferably 1:60-40: 1. The composition has obvious synergistic effect, can reduce the use amount of medicaments, reduce the cost, reduce the harm to the environment and is beneficial to delaying the generation of drug resistance of pathogenic bacteria.

Description

Bactericidal composition containing orysastrobin and shenqinmycin

Technical Field

The invention belongs to the field of agricultural chemistry, and particularly relates to a bactericidal composition containing orysastrobin and shenqinmycin.

Background

Since a long time ago, unscientific means such as unicity, continuity, repeatability and the like have caused many pathogenic bacteria to generate different degrees of resistance to currently used medicaments, and it is reported that the generation time of resistance of various medicaments is shortened year by year due to the unscientific means, resulting in great increase of prevention and treatment cost, environmental cost and medicament development cost. The pesticide prepared by combining the effective components of different pesticides is an effective and quick way for developing and researching new pesticides and preventing and controlling resistant germs in agriculture at present. The compound synergistic formula is good, can obviously improve the actual prevention and treatment effect, and reduces the using amount of pesticide, thereby greatly delaying the generation speed of drug resistance of germs, and being an important means for comprehensively preventing and treating diseases.

Orysastrobin, english name orysastrobin, chemical name: (2E) -2- (methoxyimino) -2- {2- [ (3E,5E,6E) -5- (methoxyimino) -4, 6-dimethyl-2, 8-dioxa-3, 7-dioxanonan-3, 6-dien-1-yl ] phenyl } -N-methylacetamide. The bactericide belongs to strobilurin bactericides, acts by inhibiting the respiration of pathogenic cell micro-mitochondria, and acts on Qo sites of cytochrome b, so that the transfer of electrons from cytochrome b to cytochrome c1 is prevented.

The Shenqinmycin, the name of which is Shenqinmycin in English, is a novel microbial pesticide independently developed in China, is a microbial antibiotic secreted by pseudomonas fluorescens M18, and has the main effective component of phenazine-1-carboxylic acid. The antibiotic has strong inhibiting effect on various plant pathogenic bacteria.

In the prior art, no document report is available on the combined use of orysastrobin and shenqinmycin.

Disclosure of Invention

In order to overcome the defects of large medicament dosage, serious environmental pollution, high pathogenic bacteria resistance generation speed and the like of the single bactericide, the invention provides an efficient and safe bactericide composition containing orysastrobin and shenqinmycin. Under the optimal proportion, the composition has obvious synergistic effect, reduces the using amount of medicaments under the condition of ensuring the optimal control effect, reduces the cost, reduces the harm to the environment and is beneficial to delaying the generation of drug resistance of pathogenic bacteria.

In order to solve the problems in the prior art, the invention provides the following technical scheme:

the bactericidal composition contains the active ingredients of orysastrobin and shenqinmycin, and the weight ratio of the orysastrobin to the shenqinmycin is 1:80-80: 1. The preferable ratio is 1:60-40: 1; more preferably the ratio is 1:10-10: 1.

The bactericidal composition also comprises agriculturally acceptable auxiliaries, and the weight percentage of the active ingredients is 5-80%. Preferably, the weight percentage of the active ingredients is 10-70%; more preferably 40-60%.

In the bactericidal composition, the auxiliary agent comprises a dispersing agent, an emulsifying agent, a disintegrating agent, a stabilizing agent, an antifreezing agent, a defoaming agent, a film forming agent, a wetting agent, a synergist, a penetrating agent, a filler and a solvent.

The bactericidal composition can be prepared into an agriculturally acceptable proper preparation; the preferable preparation formulation is wettable powder, missible oil, water dispersible granules, suspending agent, microemulsion or aqueous emulsion.

The bactericidal composition is used for preventing and treating crop diseases. The crops comprise wheat, rice, corn, vegetables and the like. The disease is preferably banded sclerotial blight, rice blast, sclerotinia sclerotiorum and the like. The compositions of the invention can be applied by conventional methods, such as pouring, spraying, spreading, the amount of application varying with weather conditions or crop conditions.

Compared with the prior art, the invention has the following beneficial effects:

the bactericidal composition of the invention consists of two active ingredients with different action mechanisms, has obvious synergistic effect after being mixed, improves the control effect, and is also beneficial to overcoming and delaying the generation of drug resistance of pathogenic bacteria. The composition of the invention is mixed with pesticide to reduce the dosage, thereby reducing the cost and lightening the pollution to the environment, having good safety and meeting the safety requirement of pesticide preparations. The composition of the invention expands the prevention and treatment spectrum, has a function of treating other diseases simultaneously, and can achieve the effect of treating more than one medicine.

Detailed Description

The following detailed description of specific embodiments of the present invention is provided in conjunction with examples, but it should be understood that the scope of the present invention is not limited to the specific embodiments.

Formulation example 1: 40% orysastrobin-shenqinmycin suspending agent

39% of orysastrobin, 1% of shenqinmycin, 7.5% of sodium methyl naphthalene sulfonate formaldehyde condensate, 0.5% of xanthan gum, 3% of bentonite, 600# 5% of agricultural milk, 5.5% of glycerol and the balance of water to 100%.

Formulation example 2: 24% orysastrobin-shenqinmycin suspending agent

22% of orysastrobin, 2% of shenqinmycin, 6.5% of lignosulfonic acid, 2.5% of tea seed cake powder, 4% of bentonite, 2% of magnesium aluminum silicate, 8% of ethylene glycol and the balance of water to 100%.

Formulation example 3: 10% orysastrobin-shenqinmycin aqueous emulsion

5% of orysastrobin, 5% of shenqinmycin, 15% of alpha-methylnaphthalene, 7% of phenethyl phenol polyoxyethylene ether, 8% of alkylphenol polyoxyethylene polyoxypropylene ether, 10% of ethyl phenol polyoxyethylene ether and the balance of water to 100%.

Formulation example 4: 45% orysastrobin-shenqinmycin microemulsion

5% of orysastrobin, 40% of shenqinmycin, 12% of DMF (dimethyl formamide), 6% of cyclohexanol, 10% of ethylene glycol, 5% of alkylphenol polyoxyethylene ether formaldehyde condensate and water, wherein the water is supplemented to 100%.

Formulation example 5: 50% orysastrobin-shenqinmycin water dispersible granule

1% of orysastrobin, 49% of shenqinmycin, 4% of alkyl sodium naphthalene sulfonate, 4% of calcium lignosulfonate, 3% of sodium dodecyl sulfate, 3% of ammonium sulfate and the balance of diatomite to 100%.

Formulation example 6: 55% orysastrobin-shenqinmycin wettable powder

25% of orysastrobin, 30% of shenqinmycin, 7% of calcium lignosulphonate, 2.5% of sodium dodecyl sulfate, 12% of bentonite and the balance of attapulgite to 100%.

Bioassay example 1: indoor toxicity determination method of orysastrobin and shenqinmycin on wheat sharp eyespot

Test subjects: the test wheat sheath blight germ is from diseased wheat plants in the field.

The test method comprises the following steps: hypha growth rate method. The drug to be tested is respectively set with 6 different gradient concentrations, mixed with sterilized quantitative melting PDA culture medium, poured into a culture dish to prepare a flat plate, and each concentration is repeated for 3 times by taking a drug-free flat plate as a control. Selecting a culture dish with the diameter of 9cm, pouring the culture dish into a PDA culture medium after sterilization, inoculating a test strain after condensation, placing the culture dish in a constant-temperature incubator at about 25 ℃ for culture, cutting a bacterial dish by a puncher with the diameter of 0.6cm along the edge of the cultured bacterial colony when hyphae are uniformly distributed on the culture dish, reversely transplanting the bacterial dish onto a medicament-containing culture medium by a disinfection inoculation needle, and culturing the bacterial dish at the constant temperature of 25 ℃ for 5 days. The expanded diameter of the bacterial disc is measured by a ruler cross measurement method, and compared with the expanded diameter of a control bacterial disc without applying the pesticide, the growth inhibition rate of the pesticide is obtained. The growth inhibition rate of each treatment is converted into a probability value, the concentration is converted into a logarithmic value, and a virulence equation and an inhibitory median concentration EC50 are calculated by a least square method.

The growth inhibition rate is (average diameter of expanded control bacterial disc-average diameter of expanded drug-containing bacterial disc)/average diameter of expanded control bacterial disc multiplied by 100%

Calculating the virulence index and the cotoxicity coefficient (CTC) of the medicament according to the Sun Yunpei method.

When the CTC is less than or equal to 80, the composition shows antagonism, when the CTC is more than 80 and less than 120, the composition shows additive action, and when the CTC is more than or equal to 120, the composition shows synergistic action.

Measured virulence index (ATI) ═ standard agent EC 50/test agent EC50 x 100

Theoretical virulence index (TTI) ═ A agent virulence index x percent of A in the mixture + B agent virulence index x percent of B in the mixture

Co-toxicity coefficient (CTC) ═ measured toxicity index (ATI) of the mixture/Theoretical Toxicity Index (TTI) of the mixture x 100

The results of the virulence determination are shown in table 1 below.

TABLE 1 indoor toxicity assay results of orysastrobin and shenqinmycin on wheat sharp eyespot

As shown in Table 1, the weight ratio of orysastrobin to shenqinmycin in the bactericidal composition is in the range of 1:60-40:1, and the cotoxicity coefficients are both greater than 120, so that the composition has a remarkable synergistic effect on wheat sharp eyespot.

Bioassay example 2: orysastrobin and shenqinmycin field control effect on wheat sharp eyespot

The test method comprises the following steps: referring to table 2, the test agents and the dosages are compared with clear water as a blank; at the beginning of the disease, the first spray was applied immediately and after 7 days the second application was applied, each treatment for 3 cells, 25 square meters per cell. The disease incidence is investigated and counted before the medicine is taken and 10 days after the second medicine is taken, 5 points are randomly sampled in each cell, 5 clusters are investigated in each point, 25 clusters are investigated in total, the disease incidence degree of leaf sheaths and leaves is investigated and graded, and the disease index and the prevention and treatment effect are calculated.

Grade standard: level 0: the whole plant is disease-free; level 1: the fourth leaf and the sheaths and leaves below it (the apical leaf is the first leaf); and 3, level: the third leaf and the leaf sheaths and leaves below the third leaf are attacked; and 5, stage: the second leaf and the leaf sheaths and leaves below the second leaf are attacked; and 7, stage: attack of Jianye leaf and the leaf sheaths and leaves below the Jianye leaf; and 9, stage: the whole plant is attacked and withered in advance.

Disease index (%) [ Σ (number of disease-grade disease plants × number of disease-grade steps)/(total number of investigation plants × highest number of steps) ] × 100%;

the control effect (%) is [ (control disease index-treatment disease index)/control disease index ] × 100%

Table 2 results of field control of orysastrobin and shenqinmycin against sheath blight of wheat

Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention. All percentages in the formulation are by weight. The processing technology of each preparation of the composition is the prior art, and can be changed according to different conditions.

Claims (6)

1. The bactericidal composition containing orysastrobin and shenqinmycin is characterized in that active ingredients comprise orysastrobin and shenqinmycin, and the weight ratio of the orysastrobin to the shenqinmycin is 1:60-40: 1.

2. The bactericidal composition according to claim 1, wherein the weight ratio of orysastrobin to shenqinmycin is 1:10-10: 1.

3. The bactericidal composition of any one of claims 1-2, further comprising agriculturally acceptable adjuvants, wherein the active ingredients are present in an amount ranging from 5% to 80% by weight.

4. The bactericidal composition of claim 3, wherein the adjuvants comprise dispersants, emulsifiers, disintegrants, stabilizers, antifreezes, defoamers, film formers, wetting agents, synergists, penetrants, fillers, or solvents.

5. The germicidal composition of claim 4, wherein: the dosage form of the composition which can be prepared into wettable powder, missible oil, water dispersible granules, suspending agents, micro-emulsions or aqueous emulsions.

6. Use of the bactericidal composition according to any one of claims 1 to 5 for controlling wheat sharp eyespot.