CN107432285A - A kind of cockroach killing bait and preparation method and application containing cockroach virus - Google Patents
A kind of cockroach killing bait and preparation method and application containing cockroach virus Download PDFInfo
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- CN107432285A CN107432285A CN201610361231.4A CN201610361231A CN107432285A CN 107432285 A CN107432285 A CN 107432285A CN 201610361231 A CN201610361231 A CN 201610361231A CN 107432285 A CN107432285 A CN 107432285A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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Abstract
The invention discloses a kind of cockroach killing bait and preparation method and application containing cockroach virus.Main Ingredients and Appearance is cockroach virus and cockroach pheromones in the formulation, wherein ten thousand/g of cockroach viral inclusion body content 6000-6, it is made up of by a certain percentage sucrose, corn flour, malt syrup, glycerine, sodium benzoate, calcium carbonate, sodium acetate, calcium lactate, fluorescein sodium, sodium carboxymethylcellulose, water.The present invention is to environmental organism, person poultry harmless, non-environmental-pollution, no resistance to the action of a drug, it can effectively control the generation and harm of cockroach, suitable for Code in Hazardous Special Locations such as family, hotel, office, kindergarten, school, hospital, food factory, aircrafts, it can ensure 1 year and occur without cockroach.
Description
Technical field
The present invention relates to biological insecticides, be more particularly to a kind of cockroach killing bait containing cockroach virus and preparation method and
Using.
Background technology
Cockroach is worldwide important sanitary insect pest, contaminated food, is spread disease, serious threat human health.Known to China
Indoor cockroach have 4 sections 6 belong to 13 kinds, main species have American cockroach, Groton bug, Peroplaneta fluligginosa etc..Due to traffic convenience and
Indoor temperature and humidity is suitable, and cockroach substantially covers the place in addition to the arctic, the South Pole, and reproduction speed is fast, resistance, disguised
By force, it is difficult to which cockroach is eradicated.
For cockroach prevention and cure based on chemical prevention, main formulation has the formulations such as suspending agent, missible oil, aqueous emulsion and bait formulation at present,
Suspending agent, missible oil and aqueous emulsion are mainly based on residual spray, but spray and be easy to pollution environment, threaten dispenser personnel and interior
Resident's health, also easily injure natural enemy, pet etc..And bait formulation is relative to suspending agent, missible oil, aqueous emulsion, environmental pollution
It is smaller or do not pollute, it is comparatively safe to people and animals, cockroach can also be controlled thorough.Therefore, bait formulation is the optimal agent for preventing and treating cockroach
Type.But the cockroach killing bait of chemical composition, product resistance is easy to cockroach, can all cause poisoning can the pet that eaten by mistake and people
Can, and the ovum in egg pod can not be killed, the usual chemical bait formulation roach that goes out can ensure in 3 months and occur without cockroach, it is necessary to repeatedly
Dispenser prevents and treats.In particular surroundings, such as the Code in Hazardous Special Locations such as dairy factory, food factory, cake shop, brewery, kindergarten, hospital, just not
Chemical bait formulation is easily used, can only be gone out roach using viscous roach plate or dust catcher, but the roach that goes out is not thorough.
Cockroach virus is a kind of new life virus insecticide, and it kills roach mechanism and returned for cockroach virus through cockroach feeding bait formulation
To nest, cockroach virus replicates rapidly in cockroach body, and cockroach slowly catches an illness death, around nest cockroach feeding catch an illness and dead
Cockroach corpse and the excrement with virion, the numerous and confused virus infection of cockroach in nest is gradually dead, and parent cockroach is due to dye
Disease is laid eggs, and virus can be also carried in ovum, ovum can also be poisoned to death even if hatching, therefore can reach the purpose at a brood of end.Cockroach disease
Malicious selectivity is strong, only kills cockroach, does not injure any natural enemy, environmentally safe to person poultry safety, and it is thorough to kill roach, is not likely to produce
Resistance, lasting period length, if external environment can effectively prevent external cockroach from invading, interior can be ensured again without cockroach.
It has been investigated that cockroach pheromones occupy an important position in the life cycle of cockroach, pheromones are to cockroach
Feeding, mating behavior play key influence.Cockroach is arranged by middle intestinal secretion pheromones, last pheromones with excrement
Go out external and slowly evaporate into air, cockroach pheromones can summon other cockroach aggregations, feeding, and sexually matured cockroach is also used
Pheromones mate to find spouse.Currently on the market most cockroach killing bait be all lured with simple food based on, typically
It is based on sugar, cream, starch.Lure feeding effect less desirable.The present invention believes cockroach on the basis of forefathers study
Cease plain attractant and lured with food and is combined, develop and lure the more preferably new cockroach killing bait of feeding effect, preferably solve
The problem of cockroach killing bait lures the feeding effect undesirable.
At present, bait formulation does not need organic solvent in process, so both reduces production cost, reduces environment dirt again
Dye.In process, cockroach pheromones, the food of cockroach eating are added, using thickener carmethose, in vacuum
Homogeneous is sheared in emulsifying mixer, ensures product mix uniform and smooth, and adhesion is strong, and moisture retention is good, is easy to the long-term feeding of cockroach,
Improve drug effect, it is while instant packed, be readily transported, using and storage.
The content of the invention
It is an object of the invention to provide a kind of cockroach killing bait containing cockroach virus, formula is reasonable, easy to operate, desinsection
Effect is good, can effectively control the generation and harm of cockroach for a long time.
It is another object of the present invention to provide a kind of preparation method of the cockroach killing bait containing cockroach virus, method
Simply.
It is another object of the present invention to provide it is a kind of containing cockroach virus cockroach killing bait application, using
The cockroach killing bait is prepared as killing the kit of cockroach, or kills roach preparation using it as what main effect component was prepared into.
In order to achieve the above object, the present invention uses following technical measures：
A kind of cockroach killing bait containing cockroach virus, including following components：
Every gram described of cockroach killing bait is finally individual containing cockroach virus 6000-60000.
Described cockroach virus, full name is Peroplaneta fluligginosa densovirus, belongs to Parvoviridae, densovirus subfamily, its
The morphosis of virion is in spherical icosahedron, in the same size, a diameter of 20nm；Viral nucleic acid is in wire, length 1.7
μm, molecular weight is 1.7 × 105Dal (ssDNA), genome are single stranded DNA.
Described cockroach pheromones, are extracted from cockroach excrement, and its main component is cockroach ketone, there is very strong draw to cockroach
Lure effect.
Cockroach virus, cockroach pheromones are all naturally occurring, and environmentally safe, to person poultry safety, selectivity is strong,
It is adapted to preventing and treating cockroach.Cockroach pheromones lure cockroach feeding bait formulation, and cockroach virus can infect nymph, adult, after cockroach infection,
It is slow-growing, depauperation, it is slow in action, gradually dead, the cockroach feeding around nest is caught an illness and dead cockroach and excrement,
Virus infection is dead, and cockroach is all killed.
Above-described formula, it is preferred that the preparation method of cockroach pheromones includes：
Cockroach pheromones extracting method：1 kilogram of fresh cockroach excrement, 3 kilograms of absolute ethyl alcohols are added, use high-shear emulsifying
After machine homogeneous, soak 24 hours.Then slurry is obtained into the filtrate of 2~2.5 kilograms of yellow transparents with manual filter press press filtration；
Filtrate is added into Rotary Evaporators, evaporates ethanol at 20~25 DEG C under vacuo, yellowish-brown is finally obtained and has and special disturb stink
Liquid substance be.After testing, the liquid substance includes cockroach aggregation pheromone, sex pheromone and other metabolites.
A kind of cockroach killing bait containing cockroach virus, including following components：
Every gram described of cockroach killing bait is finally individual containing cockroach virus 10000-50000.
A kind of cockroach killing bait containing cockroach virus, including following components：
Every gram described of cockroach killing bait contains 30000-40000/g of cockroach virus.
A kind of cockroach killing bait containing cockroach virus, including following preparation method：
During preparation, 1. first sucrose, maltose, sodium benzoate, calcium lactate are dissolved in hot water, it is stand-by；
2. glycerine and sodium carboxymethylcellulose are mixed；Then in 1. 2. mixed solution pours into, stirred in vacuum emulsification
Homogeneous stir about 30 minutes in machine；Corn flour and calcium carbonate are poured into vacuum emulsion mixer respectively again, continue homogeneous stirring
About 30 minutes.Then cooling water is led to, material is cooled to 25 DEG C or so in band pot, by cockroach virus liquid, cockroach pheromones, fluorescein
Sodium is well mixed to be poured into emulsification pot, and homogeneous stirs 30 minutes, and vacuumizes.It is qualified to examine, packing, you can obtain cockroach killing bait
Product.
A kind of application of the cockroach killing bait containing cockroach virus, is prepared as killing the examination of cockroach using the cockroach killing bait
Agent box, or kill roach preparation using it as what main effect component was prepared into.
The present invention compared with prior art, has advantages below and effect：
(1) cockroach virus combine with pheromones, can quickly lure cockroach feeding and virus infection, and the energy fast lifting roach that goes out is imitated
Fruit, controlled beneficial to population of cockroach；
(2) to person poultry safety, environmentally safe, resistance is not likely to produce to cockroach, is a kind of safe and non-toxic, and do not make evil
The high-performance bio cockroach killing bait that worm develops immunity to drugs.
(3) the chain roach that goes out, but can not see corpse, it is not necessary to excessive cleaning cockroach corpse, and chemical agent goes out roach, corpse
Everywhere, it is necessary to clear up.
(4) dispenser once, if external environment is handled well, prevents external cockroach from invading, and can ensure house with dispenser once substantially
Front yard whole year is without roach.But chemical Cockroach killing bait agent needs dispenser 2-3 times, family's whole year could be ensured without roach.
Embodiment
Embodiment 1-15：
A kind of cockroach killing bait containing cockroach virus, including following components：
The extracting method of described cockroach pheromones is：
Cockroach pheromones extracting method：A soaking barrel is taken, weighs and adds 1 kilogram of fresh cockroach excrement, adds 3 kilograms
Absolute ethyl alcohol, after high-shear emulsion machine homogeneous, soak 24 hours.Then by slurry with manual filter press press filtration, obtain 2~
The filtrate of 2.5 kilograms of yellow transparents.Filtrate is added into Rotary Evaporators, wine is evaporated at 20~25 DEG C under 76mm Hg vacuums
Smart solvent, finally obtaining about 10 milliliters of yellowish-brown has the special liquid substance for disturbing stink.After testing, cockroach aggregation information is included
Element, sex pheromone and other metabolites.
The cockroach virus is purchased from Bai Le bio tech ltd.
Embodiment 16：
A kind of preparation method of the cockroach killing bait containing cockroach virus, including following preparation process：
The present embodiment is illustrated exemplified by being formulated shown in embodiment 6.
1. sucrose, malt syrup, sodium benzoate, calcium lactate are dissolved in hot water, it is stand-by；
2. glycerine and sodium carboxymethylcellulose are mixed；
3. will be during 1. 2. mixed solution pour into, homogeneous stir about 30 minutes in vacuum emulsion mixer；
4. corn flour and calcium carbonate are poured into vacuum emulsion mixer respectively, continue homogeneous stir about 30 minutes.
5. logical cooling water, 25 DEG C or so are cooled to material in pot, by cockroach virus liquid, cockroach pheromones, fluorescein sodium
It is well mixed to pour into emulsification pot, vacuumize, homogeneous stirs 30 minutes.
6. it is qualified to examine, dispense, storage, for example 1 below 7.
Embodiment 17：
A kind of application of cockroach killing bait containing cockroach virus in cockroach insecticide is prepared：
1. test worm Groton bug (Blattella germanica), 10~15 days adults, female, male half and half.
2. test method
1) 30 Groton bugs are put into the square box device for closing door, stoppers and puts worm channel, after test worm recovers normal activity,
One jiao of placement, 10 grams of bait formulation to be measured in case, diagonal to place Groton bug feed, center, which is placed, fills immersion cotton balls culture dish.By
Day observation test worm death condition, and dead worm taken out, is counted, the 12nd day after Continuous Observation to bait throwing in, test should set three times and with
Upper repetition, and chow diet raising is set as blank control.Experiment answers cleaning experiment device after terminating every time, and experimental result is shown in Table 1,
Table 2 and table 4.
2) Groton bug is set to conceal place in simulation scene.100 Groton bugs are discharged in simulation scene.Treat test worm
After recovering normal activity, diagonally it is positioned in two culture dishes by the actual amount of a room bait formulation of recommendation, another is right
The culture dish for containing Groton bug is placed at angle, and a culture dish for filling immersion cotton balls all should be placed in parallel beside each culture dish.Close
Close door and window, and timing.Check daily and record dead borer population, 12d after Continuous Observation to bait throwing in, experimental result is shown in Table 3.
3. result of the test
Groton bug experimental result is killed in the cockroach killing bait room of table 1
Groton bug LT50 and regression equation are trapped and killed in the cockroach virus cockroach killing bait room of table 2
Kill Groton bug result of the test in the cockroach virus cockroach killing bait of table 3 simulation scene
4. test initial data
Groton bug experiment initial data is killed in the cockroach virus cockroach killing bait room of table 4
Note：A, for B, C group to repeat three times, D groups are control group.
Embodiment 18：
The toxicity test of cockroach killing bait：
First, acute oral toxicity test
(1) material and method
1. tested material：Cockroach killing bait formula presses the formula of embodiment 11.Using distilled water as solvent, 50% (w/v) is configured to
Suspension.
2. experimental animal：The SD rats 10 provided by Henan Province's animal experimental center, SPF levels, male and female half and half, body weight
196g~215g, it is in a good state of health, credit number：SCXK (Henan) 2010-0002, the certification of fitness number：
41003100000954, and the 3d that quarantines on this basis.Feed is provided by Henan Province's animal experimental center.Experimental animal feeding ring
Border, 21 DEG C~25 DEG C of temperature, relative temperature 50%~70%.Laboratory Animal Facility credit number：SYXK (Henan) 2012-0007.
3. test method：According to national standard GB15670-1995, on the basis of trial test, using limitation test method, i.e.,
5000mg/kgBW, will be female, each 5 of mouse great and mighty or powerful, fasting overnight.By tested material suspension, with 1.0ml/100gBW.Once
Oral gavage.Observed 2 weeks after contamination, record the weight of animals, poisoning symptom and death condition.
According to mouse death condition female, great and mighty or powerful, according to National Standard of the People's Republic of China《Agriculture chemical registration toxicology test side
Method》(GB15670-1995) acute oral toxicity grade scale and the Ministry of Agriculture《Agriculture chemical registration data requirement》3.3.2.8 middle table 2
" Pesticidal products toxicity grading and mark ", acute oral toxicity classification is made to tested material.
(2) result of the test
It is shown in Table 5.
The cockroach killing bait of table 5 is to rat acute toxicity test result
Animal poisoning symptom and death condition：
Obvious poisoning symptom is had no after female, male rat oral contamination, is observed to 14d rats without death
2nd, acute dermal toxicity is tested
(1) material and method
1. tested material：Cockroach killing bait, the formula of embodiment 11.
2. experimental animal：The SD rats 10 provided by experimental animal center of henan province, SPF levels, male and female half and half, body weight
216g~235g, it is in a good state of health, credit number：SCXK (Henan) 2010-0002, the certification of fitness number：
41003100000954, and the 3d that quarantines on this basis.Feed is provided by experimental animal center of henan province.Experimental animal feeding ring
Border；21 DEG C -25 DEG C of temperature, relative humidity 50%~70%.Laboratory Animal Facility credit number：SYXK (Henan) 2012-0007.
3. test method：According to national standard GB15670-1995, using limitation test method, measure as 5000mg/kgBW,
Tested material is directly applied into skin.24h loses hair or feathers in rat dorsum skin before administration, observes 1d, skin is without breakage.It is fixed big after weighing
Mouse, in 4 × 5 ㎝2Area quantification smears tested material, and plastic sheeting and gauze cover, immobilization with adhesive tape, with temperature after the lasting 4h of coating
Wash the medicine gone on skin.Observed 2 weeks after contamination, record the weight of animals, poisoning symptom and death condition.
According to National Standard of the People's Republic of China《Agriculture chemical registration toxicology test method》(GB15670-1995) it is anxious in
Property percutaneous toxicity grade scale and the Ministry of Agriculture《Agriculture chemical registration data requirement》3.3.2.8 middle table 2 " Pesticidal products toxicity grading and mark
Know ", acute dermal toxicity classification is made to tested material.
(2) result of the test
It is shown in Table 6
The cockroach virus cockroach killing bait of table 6 is to rat acute percutaneous toxicity experimental result
Animal poisoning symptom and death condition：
Obvious poisoning symptom is had no after mouse Skin contamination female, great and mighty or powerful, observation 14d rats are without death.
3rd, acute inhalation toxicity is tested
(1) material and method
1. tested material：Cockroach virus cockroach killing bait.
2. experimental animal：The SD rats 10 provided by experimental animal center of henan province, SPF levels, male and female half and half, body weight
207g~225g, it is in a good state of health, credit number：SCXK (Henan) 2010-0002, the certification of fitness number：
41003100000954, and the 3d that quarantines on this basis.Feed is provided by experimental animal center of henan province.Experimental animal feeding ring
Border；21 DEG C -25 DEG C of temperature, relative humidity 50%~70%.Laboratory Animal Facility credit number：SYXK (Henan) 2012-0007.
3. test method：According to national standard GB15670-1995, using limitation test method, design dosage is more than 5000mg/
㎡.Experimental animal is put into 1m3In lucite exposure in static total enclosure chamber cabinet, the cockroach virus cockroach killing bait that embodiment 11 is formulated distills
Water is configured to 2% (w/v) solution, is sprayed, fan stirring, has been sprayed in 15 minutes to toxicant exposure cabinet quantification with atomizer.Contamination
Continue 2 hours.Observed 2 weeks after contamination, record body weight, poisoning symptom and death condition.
According to National Standard of the People's Republic of China《Agriculture chemical registration toxicology test method》(GB15670-1995) middle peasant
Medicine acute inhalation toxicity grade scale and the Ministry of Agriculture《Agriculture chemical registration data requirement》3.3.2.8 middle " the Pesticidal products toxicity grading of table 2
And mark ", acute toxicity classification is made to tested material.
(2) result of the test
It is shown in Table 7.
The cockroach virus cockroach killing bait of table 7 is to rat acute toxicity on inhalation result of the test
Animal poisoning symptom and death condition：
Obvious poisoning symptom is had no after mouse Inhalation female, great and mighty or powerful, is observed to 14d rats without death.
4th, eye irritant test
(1) material and method
1. tested material：Cockroach virus cockroach killing bait.
2. experimental animal:Large ear rabbit 4, body weight 2.4kg~2.7kg, male and female half and half, positive experimental animal is herded by Beijing gold
Cultivate Co., Ltd to provide, regular grade.Credit number：SCXK (capital) 2010-0001, animal quality certification number：
1180480000096.Rabbit eyes are without exception, are in a good state of health, and the 3d that quarantines on this basis.Feed is tested dynamic by Henan Province
Thing center provides.Experimental animal feeding environment：Temperature：21 DEG C~25 DEG C, relative humidity 50%~70%.Laboratory Animal Facility is permitted
Can the number of card：SYXK (Henan) 2012-0007.
3. test method：According to national standard GB15670-1995, large ear rabbit 4, right eye is tested eye, and left eye is control.
It is intracapsular that the cockroach virus cockroach killing bait 0.1g that embodiment 11 is formulated is inserted into eye conjunctiva on the right side of rabbit, gently closes eyelid 1s, administration
Observe 1h, 24h, 48h, 72h, 4d, 5d, 7d stimulate the reaction, record conjunctiva, iris and cornea degree of injury respectively afterwards.
According to National Standard of the People's Republic of China《Agricultural chemicals light fixture toxicology test method》(GB15670-1995) eye in
Excitant grade scale judges eye injury position, calculates degree of injury integration, and makes excitant evaluation.
(2) result of the test
It is shown in Table 8
After administration：
Eye irritation score index (I.A.O.I) is 3.5；
It is 0 after average eye irritation (M.I.O.I) 48h.
The cockroach virus cockroach killing bait of table 8 is to large ear rabbit eye irritation test results test condition：Do not wash eye
5th, acute skin irritation test
(1) material and method
1. tested material：Cockroach virus cockroach killing bait.
2. experimental animal：Albino guinea-pig 4, female, male half and half, positive experimental animal is herded by Beijing gold and cultivates Co., Ltd
There is provided, regular grade.Credit number：SCXK (capital) 2010-0001, animal quality certification number：11804800000680.Body weight 265g~
281g.Guinea pig skin is without exception, is in a good state of health, and the 3d that quarantines on this basis, and feed is by experimental animal center of henan province
There is provided, experimental animal feeding environment：21 DEG C~25 DEG C of temperature, relative temperature：50%~70%.Laboratory Animal Facility licensing
Number：SYXK (Henan) 2012-0007.
3. test method：According to national standard GB15670-1995.24h before experiment, by the cropping of guinea pig back both sides, area is respectively about
6cm2.The tested material 0.5g that embodiment 11 is formulated is applied to skin on the left of cavy, and right side compares.After coating agricultural chemicals, with 2 layers of yarn
Cloth, 1 layer of covered rearing with plastic film, closed and fixed with non-stimulated adhesive plaster, apply duration 4h.During off-test, it is washed with water residual
Agricultural chemicals is stayed, observes guinea pig skin 1h, 24h, 48h, 72h stimulate the reaction after administration respectively.Erythema, oedema are recorded respectively
Situation.
According to National Standard of the People's Republic of China《Agriculture chemical registration toxicology test method》(GB15670-1995) it is anxious in
Property skin irritation grade scale, judge dermoreaction and calculate stimulate the reaction integration, make excitant evaluation.
(2) experimental result
It is shown in Table 9.
The cockroach virus cockroach killing bait of table 9 is to guinea pig acute skin irritation test result
6th, allergy (sensitization) is tested
(1) material and method
1. tested material：Cockroach virus cockroach killing bait.
2. experimental animal：Albino guinea-pig 20, female, male half and half, positive experimental animal is herded by Beijing gold and cultivates Limited Liability public affairs
Department provides, regular grade.Credit number：SCXK (capital) 2010-0001, animal quality certification number：11804800000680.Body weight 257g
~273g.Guinea pig skin is without exception, is in a good state of health, and the 3d that quarantines on this basis.Feed is by Henan Province experimental animal
The heart provides, experimental animal feeding environment：21 DEG C~25 DEG C of temperature, relative humidity 50%~70%.Laboratory Animal Facility licensing
Number：SYXK (Henan) 2012-0007.
3. test method：According to national standard GB15670-1995.The pathogenic process of contact sensitization is divided into sensitization and excites 2
Stage.
(1) sensitization contact：24h is by unhairing 3cm × 3cm on the left of back part of animal before experiment.Test example 11 is formulated
Tested material 0.2g, it is coated in side animal and goes to hair-fields, with 2 layers of gauze, 1 layer of covered rearing with plastic film, is closed and fixed with non-stimulated adhesive plaster.
Continue 6h respectively, remove patch thing.7d and 14d in kind repeats contact once.
(2) contact is excited：Carry out exciting contact after last sensitization 14d, excite preceding 24h by skin unhairings on the right side of 2 groups of animals
2cm × 2cm, every animal of test group and negative control group give tested agricultural chemicals 0.1g.Each group animal wrapping method connects with sensitization
Touch.Each group tested material is removed after 6h and observes dermoreaction at once, records erythema or oedema goals for, later observation 1 time daily, extremely
12d, because reacting without mutagens, therefore excite for 2 times and Continuous Observation is to 12d.According to National Standard of the People's Republic of China
GB15670-1995《Agriculture chemical registration toxicology test method》Middle sensitization of skin strength grading method judges sensitization intensity.
(3) positive control：Using 2,4- dinitro-chloro-benzenes as positive control, it is configured to using sesame oil and acetone (1 ﹕ 1)
1% solution, test methods and procedures are identical with test group.This test evaluation, with reference to this laboratory 2014 year positive of 2 months
Result of the test.
(2) result of the test
Experimental animal is given excite contact after, skin does not occur allergic reaction, the results are shown in Table 10.
60000/gram cockroach virus cockroach killing baits are 0% to guinea pig skin sensitization rate, and skin sensitization rate strength grading is I
Level, belongs to weak sensitizer.
The cockroach virus cockroach killing bait of table 10 is to guinea pig skin allergy result of the test
Claims (6)
1. a kind of cockroach killing bait containing cockroach virus, including following components：
Ingredients weight parts
Cockroach virus is appropriate
0.1-0.5 grams of cockroach pheromones
3-15 grams of sucrose
5-30 grams of malt syrup
10-40 grams of corn flour
10-20 grams of glycerine
0.1-0.8 grams of sodium benzoate
0.1-1.5 grams of calcium carbonate
0.8-3.5 grams of calcium lactate
0.1-1 grams of fluorescein sodium
0.5-2.5 grams of sodium carboxymethylcellulose
30-50 grams of water
Every gram described of cockroach killing bait is finally individual containing cockroach virus 6000-60000.
2. cockroach killing bait according to claim 1, described cockroach pheromones extracting method includes：1 kilogram of fresh roach
Dung beetle excrement, 3 kilograms of absolute ethyl alcohols are added, after high-shear emulsion machine homogeneous, soaked 24 hours；
Then slurry is obtained into the filtrate of 2 ~ 2.5 kilograms of yellow transparents with manual filter press press filtration；Filtrate is added into rotary evaporation
Instrument, ethanol is evaporated at 20 ~ 25 DEG C under vacuo, finally obtaining yellowish-brown has the special liquid substance for disturbing stink to be.
3. cockroach killing bait according to claim 1, including following components：
Ingredients weight parts
Cockroach virus is appropriate
0.2-0.4 grams of cockroach pheromones
5-12 grams of sucrose
10-25 grams of malt syrup
15-30 grams of corn flour
12-18 grams of glycerine
0.2-0.7 grams of sodium benzoate
0.5-1.4 grams of calcium carbonate
1.2-3.0 grams of calcium lactate
0.2-0.8 grams of fluorescein sodium
1.0-2.3 grams of sodium carboxymethylcellulose
30-50 grams of water
Every gram described of cockroach killing bait is finally individual containing cockroach virus 10000-50000.
4. cockroach killing bait according to claim 1, including following components：
Ingredients weight parts
Cockroach virus is appropriate
0.2-0.4 grams of cockroach information cellulose content
6-11 grams of sucrose
12-20 grams of malt syrup
18-28 grams of corn flour
13-17 grams of glycerine
0.3-0.6 grams of sodium benzoate
0.4-1.2 grams of calcium carbonate
1.5-2.8 grams of calcium lactate
0.3-0.7 grams of fluorescein sodium
1.1-2.2 grams of sodium carboxymethylcellulose
30-50 grams of water
Every gram described of cockroach killing bait contains 30000-40000/g of cockroach virus.
5. a kind of cockroach killing bait containing cockroach virus, including following preparation method：
1. according to described in claim 1, first sucrose, maltose, sodium benzoate, calcium lactate are dissolved in hot water, it is stand-by；
2. glycerine and sodium carboxymethylcellulose are mixed；Then in 1. 2. mixed solution pours into, in vacuum emulsion mixer
Homogeneous stir about 30 minutes；Corn flour and calcium carbonate are poured into vacuum emulsion mixer respectively again, continue homogeneous stir about 30
Minute, then lead to cooling water, material is cooled to 25 DEG C or so in band pot, and cockroach virus liquid, cockroach pheromones, fluorescein sodium are mixed
Close and uniformly pour into emulsification pot, homogeneous stirs 30 minutes, and vacuumizes, and it is qualified to examine, packing, you can obtain cockroach killing bait production
Product.
6. the cockroach killing bait described in claim 1 is preparing the application in killing roach kit or killing roach preparation.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113519557A (en) * | 2021-07-15 | 2021-10-22 | 云南京新生物科技有限公司 | Preparation method and application of cockroach and insect sand extract |
| CN113519566A (en) * | 2021-07-15 | 2021-10-22 | 云南京新生物科技有限公司 | Method for preparing cockroach aggregation pheromone extract from cockroach sand and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1318299A (en) * | 2000-04-20 | 2001-10-24 | 湖北绿洲生物技术有限公司 | Phermomone induced virus system for killing roach |
| CN1528151A (en) * | 2003-10-21 | 2004-09-15 | 武汉大学 | Biological cockroach eradication glue bait and preparing method thereof |
| CN100998344A (en) * | 2007-01-05 | 2007-07-18 | 沈宏良 | Chemical for trapping and killing cockroach and its preparing method |
| CN101803608A (en) * | 2009-02-13 | 2010-08-18 | 福建省金鹿日化股份有限公司 | Cockroach-killing gel bait and preparation method thereof |
| CN105123805A (en) * | 2015-09-08 | 2015-12-09 | 广州立白企业集团有限公司 | Cockroach attractant composition and granulating preparation method thereof |
-
2016
- 2016-05-26 CN CN201610361231.4A patent/CN107432285A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1318299A (en) * | 2000-04-20 | 2001-10-24 | 湖北绿洲生物技术有限公司 | Phermomone induced virus system for killing roach |
| CN1528151A (en) * | 2003-10-21 | 2004-09-15 | 武汉大学 | Biological cockroach eradication glue bait and preparing method thereof |
| CN100998344A (en) * | 2007-01-05 | 2007-07-18 | 沈宏良 | Chemical for trapping and killing cockroach and its preparing method |
| CN101803608A (en) * | 2009-02-13 | 2010-08-18 | 福建省金鹿日化股份有限公司 | Cockroach-killing gel bait and preparation method thereof |
| CN105123805A (en) * | 2015-09-08 | 2015-12-09 | 广州立白企业集团有限公司 | Cockroach attractant composition and granulating preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 周启发等: "蟑螂抗性与病毒杀蟑", 《中华卫生杀虫药械》 * |
| 张民照等: "《生物农药及其应用技术问答》", 31 January 2016, 中国农业大学出版社 * |
| 郏付礼等: "毒力岛灭嶂饵剂杀灭德国小镰的效果", 《航空军医》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113519557A (en) * | 2021-07-15 | 2021-10-22 | 云南京新生物科技有限公司 | Preparation method and application of cockroach and insect sand extract |
| CN113519566A (en) * | 2021-07-15 | 2021-10-22 | 云南京新生物科技有限公司 | Method for preparing cockroach aggregation pheromone extract from cockroach sand and application thereof |
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Application publication date: 20171205 |