CN107418561A - Blue-fluorescence gold nano point, preparation method and its application in bivalent cupric ion context of detection - Google Patents
Blue-fluorescence gold nano point, preparation method and its application in bivalent cupric ion context of detection Download PDFInfo
- Publication number
- CN107418561A CN107418561A CN201710510870.7A CN201710510870A CN107418561A CN 107418561 A CN107418561 A CN 107418561A CN 201710510870 A CN201710510870 A CN 201710510870A CN 107418561 A CN107418561 A CN 107418561A
- Authority
- CN
- China
- Prior art keywords
- aqueous solution
- solution
- concentration
- blue
- fluorescence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
- C09K11/58—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing copper, silver or gold
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6432—Quenching
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Nanotechnology (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Optics & Photonics (AREA)
- Crystallography & Structural Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Biophysics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Manufacturing & Machinery (AREA)
- Pathology (AREA)
- Inorganic Chemistry (AREA)
- Materials Engineering (AREA)
- Organic Chemistry (AREA)
- Luminescent Compositions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
A kind of blue-fluorescence gold nano point, preparation method and its application in bivalent cupric ion context of detection, belong to fluorogold technical field of nano material.It is to add the NaOH aqueous solution in deionized water, adds polyfunctional group macromolecular aqueous solution, then adds and contain Au3+The aqueous solution, that is, obtain large-sized golden nanometer particle aqueous solution；Add the NaOH aqueous solution thereto, the biomolecule aqueous solution containing sulfydryl collects supernatant, and by supernatant repeated centrifugation 3~5 times, finally gives the nanodot aqueous solution.The synthetic method is simple, mild condition, it is reproducible, it is easy to largely produce, product chemistry property is stable, fluorescence quantum efficiency is high, has overdelicate detection, test limit as little as 0.7nM for bivalent cupric ion, and linear relationship is presented between the fluorescence intensity of concentration and the gold nano point of bivalent cupric ion, quantitative detection can be thus carried out to bivalent cupric ion, and there is very high selectivity, the almost influence of interference-free ion.
Description
Technical field
The invention belongs to fluorogold technical field of nano material, and in particular to a kind of blue-fluorescence gold nano point, preparation side
Method and its application in bivalent cupric ion context of detection.
Background technology
Noble metal nano point, due to being totally different from the optics of conventional metals nano dot, electricity, chemical property etc.,
Causing extensive research interest (Chem.Soc.Rev., 2012,41,3594-3623) in recent years.Wherein gold nano point due to
There is easily prepared, excellent photoluminescent property and chemical stability, be widely used in fluorescence probe, fluorescence sense etc.
Field (Microchimica Acta., 2014,182,695-701).
Bivalent cupric ion is the essential trace elements of the human body, but unsuitable intake copper ion, can be triggered on the contrary serious
Body illness.When intake copper ion is excessive, metabolic disturbance, hepato-biliary function obstacle etc. can be caused.Take in content of copper ion mistake
It is few, anaemia, osteoporosis can be caused, or even influence the brain growth of infant etc..Thus, the detection to copper ion has caused
Extensive research (J.Colloid Interface Sci., 2013,396,63-68).
Because conventional probe building-up process is complicated, the reason such as limit for height, detection time length is detected, limits application.It is so anxious
It need to develop that a kind of building-up process is simple, detection takes short, the fluorescence probe of high sensitivity.
The content of the invention
It is an object of the invention to provide a kind of blue-fluorescence gold nano point, preparation method and its in bivalent cupric ion detection side
The application in face.The method comprises the steps of firstly, preparing the golden nanometer particle of bulky grain, and then being prepared using the method for etching has blue-fluorescence
Gold nano point, and then copper ion is sensitively detected.
The gold nano spot size of preparation is uniform, and diameter is less than 3nm, using fluorescent quenching mode can quantify detection copper from
Son, test limit as little as 0.7nM, and the detection method have very high selectivity for copper ion.
The preparation method of nanodot of the present invention and its as follows to the detecting step of copper ion：
1) in 20~30mL deionized waters, it (is preferably 0.1 to be separately added into 0.25~1mL concentration as 0.01~1mol/L
~1mol/L) the NaOH aqueous solution, addition concentration be 0.001~1mol/L (be preferably 0.001~0.1mol/L, further it is excellent
Elect 0.001~0.01mol/L as) polyfunctional group macromolecular aqueous solution (can be THPC, bovine serum albumin(BSA)
Deng the aqueous solution) be used as stabilizer and reducing agent, the dosage mol ratio of wherein NaOH and polyfunctional group macromolecular is 0.1~10：
1；Then solution is stirred into 5~10min under 20~30 DEG C (are preferably 25~30 DEG C), then rapidly join concentration for 1~
100mmol/L (being preferably 1~50mmol/L, more preferably 1~10mmol/L) contains Au3+The aqueous solution (can be
HAuCl4、AuCl3Deng the aqueous solution), polyfunctional group macromolecular and Au3+Dosage mol ratio be 10~1000：1；Solution is existed
Stirred under 20~30 DEG C (are preferably 25~30 DEG C) 20~60min (be preferably 20~40min, more preferably 20~
30min), that is, large-sized golden nanometer particle aqueous solution is obtained, it is stored in 4 DEG C, standby, the now concentration of golden nanometer particle
For 0.5~10mmol/L；
2) in concentration is the golden nanometer particle aqueous solution for preparing of 0.5~10mmol/L, add concentration be 1~
100mmol/L (being preferably 1~50mmol/L) the NaOH aqueous solution, it (is preferably 0.01 to add concentration as 0.01~1mol/L
~0.5mol/L, more preferably 0.01~0.05mol/L) the biomolecule aqueous solution (cysteine, the paddy Guang containing sulfydryl
Sweet peptide, the aqueous solution containing mercaptoamino acid, albumen containing sulfydryl etc.) it is used as stabilizer and etching agent；Wherein golden nanometer particle is water-soluble
The dosage mol ratio of liquid and the biomolecule containing sulfydryl is 0.01~0.1：1, NaOH and the dosage mol ratio of the biomolecule containing sulfydryl
For 0.1~1：1；Solution is stirred under 80~150 DEG C (being preferably 100~150 DEG C, more preferably 100~120 DEG C) again
Supernatant is collected in 20~30h (be preferably 20~25h), reaction in eccentric fashion after terminating, and by supernatant repeated centrifugation 3~
5 times, the supernatant finally given is the nanodot aqueous solution of the present invention；
3) take the nanodot aqueous solution 0.5mL that step 2) prepares, add 0.5mL concentration be respectively 0,2nM,
10nM, 1 μM, 50 μM, 100 μM, 150 μM, 200 μM, 300 μM, 500 μM, 1mM, 2.5mM aqueous solution containing bivalent cupric ion
(can be CuCl2、CuSO4、Cu(NO3)2Deng the aqueous solution), add 2mL deionized water solutions, above-mentioned solution be put into glimmering
Its fluorescence intensity is tested in photothermal spectroscopic analyzer.Its fluorescence spectra shows, with the increase for adding copper ion concentration, gold nano point
Fluorescence intensity is gradually reduced, and copper ion concentration test limit as little as 0.7nM is calculated, and between copper ion concentration and fluorescence intensity
Good linear relationship is presented.
4) the nanodot aqueous solution 0.5mL that step 2) prepares is taken, is firstly added the survey of 2.5mL deionized water solutions
Its fluorescence intensity is tried, its value is set to I0；It is then respectively adding 0.5mL and Cu2+Isoconcentration contains K+The aqueous solution (can be
KCl、K2SO4、KNO3Deng the aqueous solution), Na+The aqueous solution (can be NaCl, Na2SO4、NaNO3Deng the aqueous solution), Mg2+'s
The aqueous solution (can be MgCl2、MgSO4、Mg(NO3)2Deng the aqueous solution), Zn2+The aqueous solution (can be ZnCl2、ZnSO4、Zn
(NO3)2Deng the aqueous solution), Fe3+The aqueous solution (can be FeCl3、Fe2(SO4)3、Fe(NO3)3Deng the aqueous solution), add
2mL deionized waters；Above-mentioned solution is put into XRF and tests its fluorescence intensity, its value is set to I.Draw (I0- I)/I0
Column diagram, display nanodot show nanodot prepared by the present invention to the Difference test of different metal ions
Selective enumeration method can be carried out to bivalent cupric ion.
Nanodot prepared by the present invention is used to have the characteristics that the super sensitivity detection of bivalent cupric ion：Should
The synthetic method of nanodot is simple, and mild condition is reproducible, is easy to largely produce, and product chemistry property is stable, glimmering
Photo-quantum efficiency is high, has overdelicate detection, test limit as little as 0.7nM for bivalent cupric ion, and bivalent cupric ion is dense
Linear relationship is presented between degree and the fluorescence intensity of gold nano point, thus quantitative detection can be carried out to bivalent cupric ion, and should
Detection method shows very high selectivity for bivalent cupric ion, almost the influence of interference-free ion.
Brief description of the drawings
Fig. 1：Fig. 1 a are the TEM figures of the nanodot prepared by embodiment 1, and its diameter is about 1.9nm；Fig. 1 b are real
The TEM figures of the nanodot prepared by example 3 are applied, its diameter is about 2.5nm；Prepared gold nano point is circle, point
Dissipate uniformly, size is homogeneous.
Fig. 2：The detection curve of nanodot prepared by embodiment 1 for bivalent cupric ion.From different copper ions
Under concentration gold nano point fluorogram (Fig. 2 a) it can be seen that, with add copper ion concentration increase, gold nano point it is glimmering
Luminous intensity is gradually reduced, test limit as little as 0.7nM.Can from copper ion concentration and maximum fluorescence intensity numerical value mapping (Fig. 2 b)
Arrive, good linear relationship is presented between copper ion concentration and maximum fluorescence intensity.
Fig. 3：The selective enumeration method figure of nanodot prepared by embodiment 1 for bivalent cupric ion.In gold nano
It is separately added into and Cu in the point aqueous solution2+The K of isoconcentration equivalent+、Na+、Mg2+、Zn2+、Fe3+, find Cu2+Can be by gold nano point
Fluorescence intensity be quenched more than 60%, and other impurities ion has little to no effect for the fluorescence intensity of gold nano point.Show
The detection method has very high selectivity for copper ion.Wherein I0It is strong not add the gold nanoclusters fluorescence of metal ion
Degree, after I is adds respective metal ion, the fluorescence intensity of gold nanoclusters.
Embodiment
Embodiment 1
The NaOH aqueous solution that 0.25mL concentration is 1mol/L is separately added into 22.5mL deionized waters, 3 μ L concentration are
The 10mmol/L THPC aqueous solution, 5min is stirred at 30 DEG C, and it is 50mmol/L's then to add 500 μ L concentration
HAuCl4The aqueous solution, 25min is stirred at 25 DEG C, you can obtain the golden nanometer particle aqueous solution, now obtain golden nanometer particle water
Solution concentration is 1mmol/L.
The golden nanometer particle aqueous solution that 10mL is prepared is taken, adds the NaOH aqueous solution that 30 μ L concentration are 1mol/L, then add
Enter 0.0138g cysteines, 24h is stirred at 100 DEG C.Reaction collects supernatant in eccentric fashion after terminating, and by supernatant
Liquid repeated centrifugation 3 times, it is the nanodot aqueous solution of the present invention to finally give supernatant.Under transmission electron microscope, gold
Nano-dot size is 1.9nm or so, uniform particle sizes, good dispersion (Fig. 1 a).
The above-mentioned nanodot aqueous solution 0.5mL prepared is taken, it is respectively 0,2nM, 10nM, 1 to add 0.5mL concentration
μM, 50 μM, 100 μM, 150 μM, 200 μM, 300 μM, 500 μM, 1mM, 2.5mM CuCl2The aqueous solution, add 2mL deionizations
The aqueous solution.Above-mentioned solution is put into XRF and tests its fluorescence intensity.Its fluorescence spectra shows, with add copper from
The increase of sub- concentration, the fluorescence intensity of gold nano point are gradually reduced, and copper ion concentration test limit as little as 0.7nM (figures are calculated
2a).And good linear relationship (Fig. 2 b) is presented between copper ion concentration and fluorescence intensity.
6 parts of above-mentioned each 0.5mL of the nanodot aqueous solution prepared are taken, are separately added into 2.5mL deionized water solutions
Its fluorescence intensity is tested, its value is set to I0.Then the CuCl that 0.5mL concentration is 300 μM is separately added into every part of solution2、KCl、
NaCl、MgCl2、ZnCl2、FeCl3The aqueous solution, add 2mL deionized waters.Above-mentioned solution is put into XRF and surveyed
Its fluorescence intensity is tried, its value is set to I, draws (I0- I)/I0Column diagram (Fig. 3), it can be seen that Cu2+Can be by gold nano point
Fluorescence intensity is quenched more than 60%, and other impurities ion has little to no effect for the fluorescence intensity of gold nano point.Show this
Detection method has very high selectivity for copper ion.
Embodiment 2
The NaOH aqueous solution that 0.5mL concentration is 1mol/L is separately added into 25mL deionized waters, 10 μ L concentration are
10mmol/L Bovine Serum Albumin in Aqueous Solution, 5min is stirred at 25 DEG C, it is 50mmol/L's then to add 500 μ L concentration
The HAuCl4 aqueous solution, 25min is stirred at 30 DEG C.It can obtain golden nanometer particle.It is dense now to obtain the golden nanometer particle aqueous solution
Spend for 0.5mmol/L.
The golden nanometer particle that 10mL is prepared is taken, the NaOH aqueous solution that 50 μ L concentration are 1mol/L is added, adds
0.025g cysteines, 20h is stirred at 120 DEG C.Reaction collects supernatant in eccentric fashion after terminating, and by supernatant weight
Centrifuge 3 times again, it is nanodot of the present invention to finally give supernatant.Under transmission electron microscope, gold nano spot size
For 2.2nm or so, uniform particle sizes, good dispersion.
The above-mentioned nanodot 0.5mL prepared is taken, it is respectively 0,2nM, 10nM, 1 μM, 50 μ to add 0.5mL concentration
M, 100 μM, 150 μM, 200 μM, 300 μM, 500 μM, 1mM, 2.5mM CuSO4The aqueous solution, add 2mL deionized water solutions.
Above-mentioned solution is put into XRF and tests its fluorescence intensity.Its fluorescence spectra is shown, with addition copper ion concentration
Increase, the fluorescence intensity of gold nano point is gradually reduced, and copper ion concentration test limit as little as 2nM is calculated.And copper ion is dense
Good linear relationship is presented between degree and fluorescence intensity.
6 parts of above-mentioned each 0.5mL of the nanodot aqueous solution prepared are taken, are separately added into 2.5mL deionized water solutions
Its fluorescence intensity is tested, its value is set to I0.Then the CuCl that 0.5mL concentration is 300 μM is separately added into every part of solution2、KCl、
NaCl、MgCl2、ZnCl2、FeCl3The aqueous solution, add 2mL deionized waters.Above-mentioned solution is put into XRF and surveyed
Its fluorescence intensity is tried, its value is set to I, draws (I0- I)/I0Column diagram, it can be seen that Cu2+Can be strong by the fluorescence of gold nano point
Degree is quenched more than 80%, and other impurities ion has little to no effect for the fluorescence intensity of gold nano point.Show the detection side
Method has very high selectivity for copper ion.
Embodiment 3
The NaOH aqueous solution that 0.5mL concentration is 1mol/L is separately added into 25mL deionized waters, 10 μ L concentration are
The 10mmol/L THPC aqueous solution, 5min is stirred at 25 DEG C, and it is 50mmol/L's then to add 500 μ L concentration
The HAuCl4 aqueous solution, 25min is stirred at 30 DEG C.It can obtain golden nanometer particle.It is dense now to obtain the golden nanometer particle aqueous solution
Spend for 0.8mmol/L.
The golden nanometer particle that 10mL is prepared is taken, the NaOH aqueous solution that 50 μ L concentration are 1mol/L is added, adds
0.025g glutathione, 20h is stirred at 120 DEG C.Reaction collects supernatant in eccentric fashion after terminating, and by supernatant weight
Centrifuge 3 times again, it is nanodot of the present invention to finally give supernatant.Under transmission electron microscope, gold nano spot size
For 2.5nm or so, uniform particle sizes, good dispersion (Fig. 1 b).
The above-mentioned nanodot 0.5mL prepared is taken, it is respectively 0,2nM, 10nM, 1 μM, 50 μ to add 0.5mL concentration
M, 100 μM, 150 μM, 200 μM, 300 μM, 500 μM, 1mM, 2.5mM CuSO4The aqueous solution, add 2mL deionized water solutions.
Above-mentioned solution is put into XRF and tests its fluorescence intensity.Its fluorescence spectra is shown, with addition copper ion concentration
Increase, the fluorescence intensity of gold nano point is gradually reduced, and copper ion concentration test limit as little as 1.2nM is calculated.And copper ion
Good linear relationship is presented between concentration and fluorescence intensity.
6 parts of above-mentioned each 0.5mL of the nanodot aqueous solution prepared are taken, are separately added into 2.5mL deionized water solutions
Its fluorescence intensity is tested, its value is set to I0.Then the CuCl that 0.5mL concentration is 300 μM is separately added into every part of solution2、KCl、
NaCl、MgCl2、ZnCl2、FeCl3The aqueous solution, add 2mL deionized waters.Above-mentioned solution is put into XRF and surveyed
Its fluorescence intensity is tried, its value is set to I, draws (I0- I)/I0Column diagram, it can be seen that Cu2+Can be strong by the fluorescence of gold nano point
Degree is quenched more than 65%, and other impurities ion has little to no effect for the fluorescence intensity of gold nano point.Show the detection side
Method has very high selectivity for copper ion.
Claims (5)
1. a kind of preparation method of blue-fluorescence gold nanodot solution, its step are as follows：
1) in 20~30mL deionized waters, the NaOH aqueous solution that 0.25~1mL concentration is 0.01~1mol/L is added, is added dense
The polyfunctional group macromolecular aqueous solution for 0.001~1mol/L is spent as stabilizer and reducing agent, wherein NaOH and polyfunctional group
The dosage mol ratio of macromolecular is 0.1~10：1；Then solution is stirred into 5~10min at 20~30 DEG C, then rapidly joined dense
Spend and contain Au for 1~100mmol/L3+The aqueous solution, polyfunctional group macromolecular and Au3+Dosage mol ratio be 10~1000：
1；Solution is finally stirred into 20~60min at 20~30 DEG C, that is, obtains large-sized golden nanometer particle aqueous solution, Jenner's grain of rice
The concentration of son is 0.5~10mmol/L；
2) in concentration is the golden nanometer particle aqueous solution that 0.5~10mmol/L is prepared, addition concentration is 1~100mmol/L
The NaOH aqueous solution, add concentration be 0.01~1mol/L the biomolecule aqueous solution containing sulfydryl as stabilizer and etching
Agent；Wherein the dosage mol ratio of the golden nanometer particle aqueous solution and the biomolecule containing sulfydryl is 0.01~0.1：1, NaOH and containing sulfydryl
The dosage mol ratio of biomolecule is 0.1~1：1；Solution is stirred into 20~30h at 80~150 DEG C again, reaction terminate after with
The mode of centrifugation collects supernatant, and by supernatant repeated centrifugation 3~5 times, the supernatant finally given is fluorescence gold nano
The point aqueous solution.
A kind of 2. preparation method of blue-fluorescence gold nanodot solution as claimed in claim 1, it is characterised in that：Polyfunctional group
Macromolecular aqueous solution is the THPC aqueous solution or Bovine Serum Albumin in Aqueous Solution.
A kind of 3. preparation method of blue-fluorescence gold nanodot solution as claimed in claim 1, it is characterised in that：Containing sulfydryl
The biomolecule aqueous solution is aqueous cystein solution, the glutathione aqueous solution, aqueous acid containing sulfhydryl amino or albumen containing sulfydryl
The aqueous solution.
A kind of 4. blue-fluorescence gold nanodot solution, it is characterised in that：It is as the method described in claims 1 to 3 appoints item one
It is prepared.
5. blue-fluorescence gold nanodot solution described in claim 4 is in the application of bivalent cupric ion context of detection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710510870.7A CN107418561B (en) | 2017-06-29 | 2017-06-29 | Blue-fluorescence gold nano point, preparation method and its application in bivalent cupric ion context of detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710510870.7A CN107418561B (en) | 2017-06-29 | 2017-06-29 | Blue-fluorescence gold nano point, preparation method and its application in bivalent cupric ion context of detection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107418561A true CN107418561A (en) | 2017-12-01 |
| CN107418561B CN107418561B (en) | 2019-03-22 |
Family
ID=60427807
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710510870.7A Active CN107418561B (en) | 2017-06-29 | 2017-06-29 | Blue-fluorescence gold nano point, preparation method and its application in bivalent cupric ion context of detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107418561B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108732151A (en) * | 2018-05-31 | 2018-11-02 | 华南理工大学 | There is the preparation of luminous gold nanoparticle and its rapid analysis and test method of highly sensitive optic response for volatile amine |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102908633A (en) * | 2012-07-31 | 2013-02-06 | 南京大学 | Multifunctional gold and silver core-shell nanoparticles and preparation method |
| CN104383919A (en) * | 2014-09-30 | 2015-03-04 | 江南大学 | Preparation method of nanocluster mimic enzyme with visible-light activity and use of nanocluster mimic enzyme in colourimetry detection of trypsin |
| CN105199716A (en) * | 2015-09-26 | 2015-12-30 | 福建医科大学 | 3-mercaptopropoinate-bovine serum albumin-gold nanoclusters and preparation method thereof |
| CN105199717A (en) * | 2015-09-26 | 2015-12-30 | 福建医科大学 | 2-mercaptoimidazole-bovine serum albumin-gold nanocluster fluorescent material and preparation method thereof |
| CN105368447A (en) * | 2015-09-26 | 2016-03-02 | 福建医科大学 | 1-methyl-5-tetrazole-thione-bovine serum albumin-gold nanocluster fluorescent material and preparation method thereof |
-
2017
- 2017-06-29 CN CN201710510870.7A patent/CN107418561B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102908633A (en) * | 2012-07-31 | 2013-02-06 | 南京大学 | Multifunctional gold and silver core-shell nanoparticles and preparation method |
| CN104383919A (en) * | 2014-09-30 | 2015-03-04 | 江南大学 | Preparation method of nanocluster mimic enzyme with visible-light activity and use of nanocluster mimic enzyme in colourimetry detection of trypsin |
| CN105199716A (en) * | 2015-09-26 | 2015-12-30 | 福建医科大学 | 3-mercaptopropoinate-bovine serum albumin-gold nanoclusters and preparation method thereof |
| CN105199717A (en) * | 2015-09-26 | 2015-12-30 | 福建医科大学 | 2-mercaptoimidazole-bovine serum albumin-gold nanocluster fluorescent material and preparation method thereof |
| CN105368447A (en) * | 2015-09-26 | 2016-03-02 | 福建医科大学 | 1-methyl-5-tetrazole-thione-bovine serum albumin-gold nanocluster fluorescent material and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| CHIH‐CHING HUANG等: "Synthesis of Highly Fluorescent Gold Nanoparticles for Sensing Mercury(II)", 《ANGEW. CHEM.》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108732151A (en) * | 2018-05-31 | 2018-11-02 | 华南理工大学 | There is the preparation of luminous gold nanoparticle and its rapid analysis and test method of highly sensitive optic response for volatile amine |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107418561B (en) | 2019-03-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Gao et al. | Sub-nanometer sized Cu 6 (GSH) 3 clusters: one-step synthesis and electrochemical detection of glucose | |
| CN105199718B (en) | Red fluorescent gold/copper nanocluster alloy as well as preparation method and application thereof | |
| Meng et al. | Turn-on fluorescence detection of ascorbic acid with gold nanolcusters | |
| Li et al. | Nitrogen doped carbon dots derived from natural seeds and their application for electrochemical sensing | |
| Sung et al. | Analysis of gold and silver nanoparticles internalized by zebrafish (Danio rerio) using single particle-inductively coupled plasma-mass spectrometry | |
| Wang et al. | Simple synthesis of silver nanoparticles functionalized cuprous oxide nanowires nanocomposites and its application in electrochemical immunosensor | |
| CN110618123B (en) | Efficient surface-enhanced Raman scattering substrate material and preparation method thereof | |
| Costa et al. | High performance gold nanorods and silver nanocubes in surface-enhanced Raman spectroscopy of pesticides | |
| Li et al. | Honeycomb-like AgNPs@ TiO2 array SERS sensor for the quantification of micro/nanoplastics in the environmental water samples | |
| Zhang et al. | Effects of bipyramidal gold nanoparticles and gold nanorods on the detection of immunoglobulins | |
| CN107418561B (en) | Blue-fluorescence gold nano point, preparation method and its application in bivalent cupric ion context of detection | |
| Vijayakumar | In vitro stability studies on gold nanoparticles with different stabilizing agents | |
| Anh et al. | Promoting electron transfer kinetics and adsorption capacity for the detection of furazolidone in real food samples by using ag-core@ Fe3O4-shell-based electrochemical sensing platform | |
| Gao et al. | High-efficiency mechanically assisted alkaline extraction of nanoparticles from biological tissues for spICP-MS analysis | |
| Huo et al. | Stable and oxidant responsive zwitterionic nanoclusters | |
| Jin et al. | In situ reduction triggers the highly sensitive detection of pesticide by classic gold nanoparticle and quantum dots nanocomposite | |
| Kanaki et al. | Using nanoparticles to determine the transport efficiency of microflow and nanoflow nebulizers in inductively coupled plasma-mass spectrometry | |
| CN104623693B (en) | Preparation method of fluorescent nano-composite having drug-release function | |
| Xie et al. | Synergistic enhancement of ultrahigh SERS activity via Cu2O@ Ag Core-Shell structure for accurate label-free identification of breast tumor subtypes | |
| CN105642912B (en) | A kind of preparation method and application of golden nanometer particle | |
| Krystek et al. | Exploring methods for compositional and particle size analysis of noble metal nanoparticles in Daphnia magna | |
| Liu et al. | Label-free and sensitive detection of microalgae protein using GNRs-based resonance light scattering system | |
| CN107643337A (en) | A kind of matrix and preparation method thereof, biology sample detection method | |
| CN106896096A (en) | A kind of separator, preparation method and applications with SERS activity | |
| Adura et al. | Ion pair method to determine the CTAB content in gold nanorods samples |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |