CN107402293A - A kind of experimental provision for promoting Osteoblast Differentiation based on titanium alloy elastic deformation - Google Patents
A kind of experimental provision for promoting Osteoblast Differentiation based on titanium alloy elastic deformation Download PDFInfo
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- CN107402293A CN107402293A CN201710594660.0A CN201710594660A CN107402293A CN 107402293 A CN107402293 A CN 107402293A CN 201710594660 A CN201710594660 A CN 201710594660A CN 107402293 A CN107402293 A CN 107402293A
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Abstract
The invention provides a kind of experimental provision for promoting Osteoblast Differentiation based on titanium alloy elastic deformation, the experimental provision is mainly formed by applying stressed pressure head, top board, titanium alloy thin slice, lower bottom base, six orifice plates and support platform, and wherein top board and lower bottom base has fixed card slot.Specifically, mesenchymal stem cells MSCs is inoculated on titanium alloy thin slice by experiment, then titanium alloy thin slice is stuck between top board and lower bottom base, its entirety is positioned in six orifice plates, and covered with sterile shrouding film, finally six orifice plates are positioned in support platform and using the reciprocal compression of pressure head loaded cycle, so as to reach the experiment purpose for promoting the mesenchymal stem cells MSCs being adhered on titanium alloy material to break up to skeletonization direction.
Description
Technical field
The present invention relates to a kind of experimental provision, and in particular to a kind of reality for promoting Osteoblast Differentiation based on titanium alloy elastic deformation
Experiment device, belong to field of biomedicine technology.
Background technology
Titanium metal material because its intensity is high, modulus of elasticity is low, good biocompatibility and turn into the most frequently used implant of clinic
One of material.Such as joint prosthesis revision procedure defect reconstruction, spinal vertebral in Cranial defect reconstruction operations caused by a variety of causes
Complete to cut or secondary full resection, the postoperative defect reconstruction of tumor resection etc., needing one kind badly can have again with bone tissue good integration
The filler or supporter of enough enabling capabilities, titanium alloy material are undoubtedly one of optimal selection, and the heat of Recent study
The introduction and application of point, particularly direct metal 3D printing technique, make the application prospect of titanium alloy more wide.
Non-linear elastic deformation can occur under appropriate stress stimulation for titanium alloy, during human body walking with load, circulation
Reciprocal stress can cause titanium alloy support to produce extremely trickle elastic deformation.Mesenchymal stem cells MSCs is that mechanical signal is quick
Sense and response cell, research show in cell and the various mechanical signals of substrate (mechanical strain, fluid shear stress, sound state
Compression etc.) specific power transduction pathway can be activated by stem cell surface receptor to adjust the differentiation of stem cell.
The research for responding various mechanical signals for mesenchymal stem cells MSCs in the past is mainly reinforced using special cell
Instrument, such as Flexcell cyto-mechanics culture systems.The system can provide different types of mechanical stimulation to cell, illustrate body
The relation of the biological behaviours such as the propagation of outer cell, differentiation, apoptosis and mechanical stimulation, illustrate cell to mechanical signal impression and
The mechanism of response.In the experimental provision, cell growth is stretched on the soft elastic substrates film of Tissue Culture Plate by stretching
Elastic substrates film makes attached cell be produced deformation by mechanical stimulation, but the basilar memebrane is silica gel content, can not be simulated very well
Metal implant-bone interface integrates environment, therefore this experiment test is mainly limited to theoretical research.
In clinical practice, the biological characteristics of titanium alloy implant belongs to hard base material, ground close to people's bone tissue
Study carefully and find that substrate hardness is that medulla mesenchyma is done on hard base material an important factor for influenceing mesenchymal stem cells differentiation
Cell is easier to break up to skeletonization direction, and stem cell is easier to break up into directions such as fat on flexible subserate material.It is above-mentioned special
Cell augmentor, its elastic substrates film is softer, and one of key constraints of experimental study.
In order to promote titanium alloy implant-bone interface integration and osteanagenesis, research master of the tradition for titanium alloy implant
Concentrate on alloying component improvement, the control of support pore size, porosity communication rate and metal surface modification etc..Closed for titanium
The research of golden physical deformation and bone tissue regeneration relation, is not reported so far.
The content of the invention
The technical problems to be solved by the invention are：A kind of experimental provision is provided, it is thin to titanium alloy by the experimental provision
Piece applies the compression to move in circles, titanium alloy thin slice is produced micro deformation, and then make to be adhered to titanium alloy sheet surface
Mesenchymal stem cells MSCs is produced deformation by mechanical stimulation, so as to reach the experiment mesh for promoting stem cell to break up to skeletonization direction
's.
In order to solve the above-mentioned technical problem, Osteoblast Differentiation is promoted based on titanium alloy elastic deformation the invention provides a kind of
Experimental provision, experimental provision are made up of pressure head, top board, titanium alloy thin slice, lower bottom base, six orifice plates and support platform, six orifice plates
It is placed in support platform, lower bottom base and top board are placed in one of hole of six orifice plates, and the lower surface of top board is provided with
One neck, the upper surface of lower bottom base are provided with the second neck, and titanium alloy thin slice is placed on the sky that top board and lower bottom base surround vertically
In, and the both sides up and down of titanium alloy thin slice are located in the first neck and the second neck respectively, pressure head is placed in the top of top board.
Further, top board carries pit, and lower bottom base carries column, and pit and column cooperatively form space.
Further, pressure head is the cylinder made of stainless steel material, and cylinder is divided into integrally formed top half
And the latter half, the diameter of top half are less than the diameter of the latter half, top half carries a threaded hole.
Further, four galianconism that top board is distributed by central cylinder and surrounding cross-shaped symmetrical are formed, the first card
Groove respectively carries a pit by the symmetrical galianconism lower section of central cylinder and two of which, two other galianconism simultaneously.
Further, lower bottom base is in the form of annular discs, and the second neck is located on the diameter line of disk, disk both sides symmetrical two
Bar column, the line of two columns and the second neck are perpendicular.
Further, six orifice plates are made up of high-molecular polythene material.
Further, support platform is aluminum alloy materials, in rectangular shape.
Further, top board, titanium alloy thin slice, lower bottom base are all by Ti6Al4V materials are made.
Further, titanium alloy thin slice is rectangle, a length of 2.9cm, a width of 1.6cm, thickness 0.1mm.
Beneficial effects of the present invention：Environment is integrated using titanium alloy material adherent cell simulation metal implant-bone interface.
Brief description of the drawings
Fig. 1 is the cutting structural representation of the experimental provision of a specific embodiment of the invention；
Fig. 2 is the overlooking the structure diagram of the experimental provision of a specific embodiment of the invention；
Fig. 3 is the overall structure diagram of the experimental provision of a specific embodiment of the invention.
Marginal data：1- pressure heads；2- top boards；The orifice plates of 3- six；4- columns；5- lower bottom bases；6- support platforms；7- titanium alloys
Thin slice.
Embodiment
In order to facilitate the understanding of the purposes, features and advantages of the present invention, below in conjunction with the accompanying drawings to the present invention
Embodiment be described in detail.It should be noted that accompanying drawing of the present invention uses using simplified form and non-essence
Accurate ratio, only for the purpose of facilitating and clarifying the purpose of the embodiments of the invention.
The present invention relates to a kind of experimental provision for promoting Osteoblast Differentiation based on titanium alloy elastic deformation.The experimental provision is main
Formed by applying stressed pressure head, top board, titanium alloy thin slice, lower bottom base, six orifice plates and support platform, wherein top board is with
Base has fixed card slot.Specifically, test and mesenchymal stem cells MSCs is inoculated on titanium alloy thin slice, then close titanium
Golden thin slice is stuck between top board and lower bottom base, and its entirety is positioned in six orifice plates, and is covered with sterile shrouding film, finally will
Six orifice plates are positioned in support platform and using the reciprocal compression of pressure head loaded cycle, titanium alloy are adhered to so as to reach promotion
The experiment purpose that mesenchymal stem cells MSCs on material breaks up to skeletonization direction.
As shown in figure 1, the experimental provision of the present invention is by pressure head 1, top board 2, titanium alloy thin slice 7, lower bottom base 5, six orifice plates 3
Formed with support platform 6, wherein top board 2 and lower bottom base 5 have fixed card slot.
Pressure head 1 is stainless steel material, in cylindrical shape.Upper part cylinder diameter is 2cm, is highly 2.5cm, wherein on
There are an a diameter of 5mm, deep 1cm screwed hole in part cylindrical center.Lower part cylinder diameter is 2.5cm, is highly 2cm,
Cylinder is seamless up and down connects, and is monolithically fabricated pressure head 1.
Top board 2 is Ti6Al4V materials, it is made up of four galianconism of cylinder and surrounding the cross-shaped symmetrical distribution in center.
Central cylinder a diameter of 1.6cm, thickness 0.3cm, it is 3.4cm to have a length on the diameter line of cylinder lower part, wide
Spend and pass through the lower section of two symmetrical galianconism simultaneously for 0.15mm, deep 0.2cm fixed card slot, the neck.Cylinder circumference
Cross-shaped symmetrical is distributed four galianconism, galianconism long 0.8cm, wide 0.8cm, thickness 0.3cm, wherein another two symmetrical galianconism
It is upper to have an a diameter of 0.3cm, the highly cylindrical hole for 0.3cm respectively.
Titanium alloy thin slice 7 is Ti6Al4V materials, are rectangle.A length of 2.9cm, a width of 1.6cm, thickness 0.1mm.
Lower bottom base 5 is Ti6Al4V materials, it is in the form of annular discs.Disk diameter is 3.4cm, thickness 0.3cm, in base upper part
Diameter line on have an a length of 3.4cm, width 0.15mm, depth is 0.2cm fixed card slot.Symmetrical two of disk both sides
A diameter of 0.29cm, high 1.8cm cylindrical column 4, two column lines and the fixing card line of rabbet joint are perpendicular.Wherein followed in application
During the reciprocal compression of ring, the column 4 of lower bottom base 5 can be inserted into the hole of top board 2, to ensure titanium alloy thin slice 7 only vertical
Direction produces deformation.
Six orifice plates 3 are the cell culture instrument that laboratory is commonly used, and are high-molecular polythene material, and bottom hole portion is smooth, sterile,
Hole internal diameter is 3.45cm, is highly 1.8cm.
Support platform 6 is aluminum alloy materials, in rectangular shape.A length of 18cm, a width of 12cm, a height of 7cm.
The specific experiment operating procedure of the experimental provision of the practical present invention is：Mesenchymal stem cells MSCs is inoculated in by experiment
On titanium alloy thin slice 7, then titanium alloy thin slice is stuck between top board 2 and lower bottom base 5, its entirety is positioned over six orifice plates 3
In, and covered, finally six orifice plates 3 are positioned in support platform 6 and reciprocal using the loaded cycle of pressure head 1 with sterile shrouding film
Compression, so as to reach the experiment for promoting the mesenchymal stem cells MSCs being adhered on titanium alloy thin slice 7 to break up to skeletonization direction
Purpose.
The present invention has advantages below：
1st, metal implant-bone interface is simulated using titanium alloy material adherent cell and integrates environment.
In clinical practice, Metallic orthopaedic implants are directly in contact with osseous tissue interface, are adhered to, grown into and newly by osteocyte
Bon e formation reaches the purpose that metal implant-bone interface is integrated, and contributes to the stability that implants early stage is fixed and at a specified future date.This reality
Experiment device uses the carrier that titanium alloy thin slice grows as cell adherence, tests mesenchymal stem cells MSCs being inoculated in titanium alloy
The change of the biological behaviour such as sheet surface, stem cell grown on titanium alloy material surface, ripe, propagation and differentiation, this
Kind of simulation more conforms to internal titanium alloy implant-bone tissue and directly contacted with each other the integration environment of effect.
2nd, titanium alloy material provides higher substrate hardness.
Research finds that substrate hardness is the bone on hard base material an important factor for influenceing mesenchymal stem cells differentiation
Bone marrow-drived mesenchymal stem is easier to break up to skeletonization direction, and stem cell is easier to divide into directions such as fat on flexible subserate material
Change.Titanium alloy material belongs to hard base material, the biology such as its biocompatibility, modulus of elasticity, physics characteristic and people's bone tissue
It is close.This experiment can be that cell adherence, grappling, growth etc. provide high substrate hardness using titanium alloy thin slice as experiment material,
Advantageously break up in mesenchymal stem cells MSCs to skeletonization direction.
3rd, micro- deformation is produced by titanium alloy thin slice and induces mesenchymal stem cells MSCs to the differentiation of skeletonization direction.
In order to promote titanium alloy implant-bone interface to integrate and osteanagenesis, the previously research master for titanium alloy implant
Concentrate on alloying component improvement, the control of support pore size, porosity communication rate and metal surface modification etc..Should in clinic
In, the process such as human body walking with load, which produces the compression to move in circles, can cause titanium alloy implant to produce trickle elastic shape
Become.But the mechanical signal caused by titanium alloy elastic deformation whether can influence to be adhered to the mesenchymal stem cells MSCs on its surface to
Skeletonization direction breaks up, and how to control this mechanical signal cell is not reported so far to the differentiation of osteanagenesis direction is advantageous to.This
Experimental provision makes titanium alloy thin slice produce micro elastic deformation, passes through mechanotransduction by applying the compression to move in circles
The cell for making to be adhered to titanium alloy sheet surface produces deformation, so as to probe into influence of the mechanical signal to cell behaviors and
The mechanism of cyto-mechanics signal impression response, to reach the experiment mesh for promoting mesenchymal stem cells MSCs to break up to skeletonization direction
's.
Preferred embodiment of the invention described in detail above.It should be appreciated that one of ordinary skill in the art without
Creative work can is needed to make many modifications and variations according to the design of the present invention.Therefore, all technologies in the art
Personnel are available by logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea
Technical scheme, all should be in the protection domain being defined in the patent claims.
Claims (9)
1. it is a kind of based on titanium alloy elastic deformation promote Osteoblast Differentiation experimental provision, it is characterised in that the experimental provision by
Pressure head, top board, titanium alloy thin slice, lower bottom base, six orifice plates and support platform are formed, and six orifice plate is placed in the support platform
On, the lower bottom base and the top board are placed in one of hole of six orifice plate, and the lower surface of the top board is opened
There is the first neck, the upper surface of the lower bottom base is provided with the second neck, and the titanium alloy thin slice is placed on the top board vertically
In the space surrounded with the lower bottom base, and the both sides up and down of the titanium alloy thin slice are respectively positioned at first neck and described
In second neck, the pressure head is placed in the top of the top board.
2. a kind of experimental provision for promoting Osteoblast Differentiation based on titanium alloy elastic deformation according to claim 1, its feature
It is, the top board carries pit, and the lower bottom base carries column, and the pit and the column cooperatively form the sky
Between.
3. a kind of experimental provision for promoting Osteoblast Differentiation based on titanium alloy elastic deformation according to claim 1, its feature
It is, the pressure head is the cylinder made of stainless steel material, and the cylinder is divided into integrally formed top half with
Half part, the diameter of the top half are less than the diameter of the latter half, and the top half carries a threaded hole.
4. a kind of experimental provision for promoting Osteoblast Differentiation based on titanium alloy elastic deformation according to claim 1, its feature
It is, four galianconism that the top board is distributed by central cylinder and surrounding cross-shaped symmetrical are formed, and first neck is same
When by the symmetrical galianconism of the central cylinder and two of which below, two other described galianconism respectively carries one
The individual pit.
5. a kind of experimental provision for promoting Osteoblast Differentiation based on titanium alloy elastic deformation according to claim 1, its feature
It is, the lower bottom base is in the form of annular discs, and second neck is located on the diameter line of the disk, and the disk both sides are symmetrically divided
Two columns of cloth, the line of two columns and second neck are perpendicular.
6. a kind of experimental provision for promoting Osteoblast Differentiation based on titanium alloy elastic deformation according to claim 1, its feature
It is, six orifice plate is made up of high-molecular polythene material.
7. a kind of experimental provision for promoting Osteoblast Differentiation based on titanium alloy elastic deformation according to claim 1, its feature
It is, the support platform is aluminum alloy materials, in rectangular shape.
8. a kind of experimental provision for promoting Osteoblast Differentiation based on titanium alloy elastic deformation according to claim 1, its feature
It is, the top board, the titanium alloy thin slice, the lower bottom base are all by Ti6Al4V materials are made.
9. a kind of experimental provision for promoting Osteoblast Differentiation based on titanium alloy elastic deformation according to claim 1, its feature
It is, the titanium alloy thin slice is rectangle, a length of 2.9cm, a width of 1.6cm, thickness 0.1mm.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201217661Y (en) * | 2008-03-28 | 2009-04-08 | 天津理工大学 | Double-frequency loading bioreactor for artificial cartilage construction |
| CN103893826A (en) * | 2014-03-03 | 2014-07-02 | 重庆大学 | Titanium alloy surface modification method for regulating and controlling stem cell differentiation and promoting in-vivo bone formation |
| CN103992947A (en) * | 2014-05-27 | 2014-08-20 | 中国人民解放军第四军医大学 | Loading device and method for pressure stress of skeletal cells in porous titanium alloy |
| CN207036850U (en) * | 2017-07-20 | 2018-02-23 | 上海交通大学医学院附属第九人民医院 | A kind of experimental provision for promoting Osteoblast Differentiation based on titanium alloy elastic deformation |
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- 2017-07-20 CN CN201710594660.0A patent/CN107402293B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201217661Y (en) * | 2008-03-28 | 2009-04-08 | 天津理工大学 | Double-frequency loading bioreactor for artificial cartilage construction |
| CN103893826A (en) * | 2014-03-03 | 2014-07-02 | 重庆大学 | Titanium alloy surface modification method for regulating and controlling stem cell differentiation and promoting in-vivo bone formation |
| CN103992947A (en) * | 2014-05-27 | 2014-08-20 | 中国人民解放军第四军医大学 | Loading device and method for pressure stress of skeletal cells in porous titanium alloy |
| CN207036850U (en) * | 2017-07-20 | 2018-02-23 | 上海交通大学医学院附属第九人民医院 | A kind of experimental provision for promoting Osteoblast Differentiation based on titanium alloy elastic deformation |
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