CN107400163A - 一种通过光照合成蛋白‑银纳米异质二聚体的方法 - Google Patents
一种通过光照合成蛋白‑银纳米异质二聚体的方法 Download PDFInfo
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- 239000002105 nanoparticle Substances 0.000 title claims abstract description 34
- 229910052709 silver Inorganic materials 0.000 title claims abstract description 22
- 239000004332 silver Substances 0.000 title claims abstract description 22
- 239000000833 heterodimer Substances 0.000 title claims abstract description 19
- 238000000034 method Methods 0.000 title claims abstract description 9
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 9
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 8
- 238000005286 illumination Methods 0.000 title claims abstract description 7
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 title abstract description 8
- 102000034238 globular proteins Human genes 0.000 claims abstract description 5
- 108091005896 globular proteins Proteins 0.000 claims abstract description 5
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims description 6
- 102000008192 Lactoglobulins Human genes 0.000 claims description 4
- 108010060630 Lactoglobulins Proteins 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 230000003760 hair shine Effects 0.000 claims description 3
- 229910001961 silver nitrate Inorganic materials 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 239000003643 water by type Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims 1
- 239000000539 dimer Substances 0.000 abstract description 7
- 230000015572 biosynthetic process Effects 0.000 abstract description 6
- 239000002082 metal nanoparticle Substances 0.000 abstract description 6
- 238000003786 synthesis reaction Methods 0.000 abstract description 6
- 229910000510 noble metal Inorganic materials 0.000 abstract description 3
- 238000007540 photo-reduction reaction Methods 0.000 abstract description 2
- 238000010276 construction Methods 0.000 description 3
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- 238000005516 engineering process Methods 0.000 description 2
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Abstract
一种通过光照合成蛋白‑银纳米异质二聚体的方法，属于分析化学技术领域。本发明通过光照合成蛋白‑银纳米异质二聚体的方法，首先形成球状的蛋白纳米粒子，然后光照还原银，最终形成蛋白‑银纳米异质二聚体。本发明采用简单便捷的方法，合成高产率并且形貌规则的异质二聚体，克服了传统的合成纳米异质二聚体步骤繁琐、合成条件严苛且难以控制的缺点，并且创新性地将有机纳米粒子和无机纳米粒子结合为一个整体，拓宽了蛋白和贵金属纳米粒子的应用范围和前景。
Description
技术领域
本发明涉及一种通过光照合成蛋白-银纳米异质二聚体的方法，属于分析化学技术领域。
背景技术
纳米二聚体由两个相对独立的结构单元连接在一起形成，当这两个结构单元组份和性质不同时，所组成的二聚体就可以同时具备二者的性质，如果这两个结构单元能够相互作用就有可能诱导出新的性质，从而使纳米二聚体成为一类新的纳米结构。在实际合成中，把两种不同结构单元直接放在一起形成的二聚体叫做异质二聚体，常见的纳米异质二聚体是将金属纳米粒子、半导体纳米粒子、磁性纳米粒子等中的两种组合起来。纳米异质二聚体的合成具有挑战性，两种不同的物质更倾向于形成核－壳结构。
蛋白是生物体的组成部分，在生物体中承载了各种重要的功能，机体的重要组织中均有不同结构和功能的蛋白存在。β-乳球蛋白具有凝胶、乳化等特性，在食品工业中有广泛的应用，同时，作为脂质运载蛋白，能与多种配体结合，作为特定的载体，在生物活性物质的运载和释放方面有广阔的应用前景。
金属纳米粒子具有独特的光学、电学、催化性能，因而受到广泛的关注。银纳米粒子作为一种贵金属纳米粒子，广泛的应用于催化剂材料、电极材料、导电材料等。功能化的银纳米粒子大大提高了其生物相容性，可应用于基因诊断和生物传感器。
将蛋白与金属纳米粒子结合成一个整体，有助于金属纳米粒子进入机体，并发挥相应的作用，提高了生物应用价值，拓宽了纳米材料技术的发展和功能开发范围。
发明内容
本发明的目的是克服现有技术中关于异质二聚体合成方法的不足，将有机与无机纳米粒子相结合，提供了一种基于光照制备高产率、形貌规则的蛋白-银纳米异质二聚体的合成方法。
本发明的技术方案：一种通过光照合成蛋白-银纳米异质二聚体的方法，首先形成球状的蛋白纳米粒子，然后光照还原银，最终形成蛋白-银纳米异质二聚体，具体步骤如下：
1）合成球状的蛋白纳米粒子：称取5-10mg β-乳球蛋白，将其溶于10mL超纯水，搅拌（500rmp）均匀，并调节pH为1.5-2；置于90℃左右水浴中搅拌反应1-1.5h，取出后搅拌冷却至室温，即得到球状的蛋白纳米粒子；
（2）合成蛋白-银纳米异质二聚体：取1mL步骤（1）中的溶液，向其中滴加80-100mmol/L的硝酸银溶液100 -200μL，用波长为405nm的激光照20-30min，即得到蛋白-银纳米异质二聚体。
本发明的有益效果：本发明采用简单便捷的方法，合成高产率并且形貌规则的异质二聚体，克服了传统的合成纳米异质二聚体步骤繁琐、合成条件严苛且难以控制的缺点，并且创新性地将有机纳米粒子和无机纳米粒子结合为一个整体，拓宽了蛋白和贵金属纳米粒子的应用范围和前景。
附图说明
图1是蛋白纳米粒子的电镜照片。
图2是蛋白-银纳米异质二聚体的电镜照片。
图3是蛋白-银纳米异质二聚体的CD图。
具体实施方式
实施例1合成蛋白-银纳米异质二聚体
（1）合成球状的蛋白纳米粒子：称取5-10mg β-乳球蛋白，将其溶于10mL超纯水，搅拌（500rmp）均匀，并调节pH为1.5-2；置于90℃左右水浴中搅拌反应1-1.5h，取出后搅拌冷却至室温，即得到球状的蛋白纳米粒子；
（2）合成蛋白-银纳米异质二聚体：取1mL步骤（1）中的溶液，向其中滴加80-100mmol/L的硝酸银溶液100 -200μL，用波长为405nm的激光照20-30min，即得到蛋白-银纳米异质二聚体。
Claims (1)
1.一种通过光照合成蛋白-银纳米异质二聚体的方法，其特征在于步骤如下：
（1）合成球状的蛋白纳米粒子：称取5-10mg β-乳球蛋白，将其溶于10mL超纯水，500rmp搅拌均匀，并调节pH为1.5-2；置于90℃水浴中搅拌反应1-1.5h，取出后搅拌冷却至室温，即得到球状的蛋白纳米粒子；
（2）合成蛋白-银纳米异质二聚体：取1mL步骤（1）中的溶液，向其中滴加80-100mmol/L的硝酸银溶液100 -200μL，用波长为405nm的激光照20-30min，即得到蛋白-银纳米异质二聚体。
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103665894A (zh) * | 2013-11-11 | 2014-03-26 | 青岛佰众化工技术有限公司 | 一种纳米丝素蛋白- 纳米银抗菌复合材料 |
| CN105664225A (zh) * | 2016-02-24 | 2016-06-15 | 苏州思彬纳米科技有限公司 | 一种纳米银、壳聚糖和蚕丝蛋白复合生物敷料及其制备方法 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103665894A (zh) * | 2013-11-11 | 2014-03-26 | 青岛佰众化工技术有限公司 | 一种纳米丝素蛋白- 纳米银抗菌复合材料 |
| CN105664225A (zh) * | 2016-02-24 | 2016-06-15 | 苏州思彬纳米科技有限公司 | 一种纳米银、壳聚糖和蚕丝蛋白复合生物敷料及其制备方法 |
Non-Patent Citations (2)
| Title |
|---|
| 周华: "大豆分离蛋白-纳米银复合物和大豆分离蛋白原子转移自由基聚合的研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
| 黄颖: "β-乳球蛋白纤维化过程中的界面及乳化性质研究", 《食品科学》 * |
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