CN107397608A - It is a kind of using the unconventional method for breeding fine breed cow of embryo biotechnology - Google Patents
It is a kind of using the unconventional method for breeding fine breed cow of embryo biotechnology Download PDFInfo
- Publication number
- CN107397608A CN107397608A CN201710419760.XA CN201710419760A CN107397608A CN 107397608 A CN107397608 A CN 107397608A CN 201710419760 A CN201710419760 A CN 201710419760A CN 107397608 A CN107397608 A CN 107397608A
- Authority
- CN
- China
- Prior art keywords
- embryo
- cow
- biotechnology
- existed
- fine breed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Reproductive Health (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Transplantation (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of using the unconventional method for breeding fine breed cow of embryo biotechnology, outstanding and suitable breast milk Niu Zuowei donors are selected, the described method comprises the following steps：A, superfecundation, b, artificial insemination c, embryo, d, embryo inspection, e, embryo cryopreservation, f, embryo thawing, g, embryo's separation, h, embryo transfer are rushed.The above method is taken, the birth rate of female calf can be efficiently controlled, and reduces production cost, improves the quality of milk cow and milk, has reached the purpose of extraordinary fast breeding breast milk ox.
Description
Technical field
The present invention relates to a kind of biotechnology, is specially that one kind breeds breeding milk using embryo biotechnology is unconventional
The method of ox.
Background technology
A kind of one of milk food most comprehensive and perfect as nutrition, is always valued by the people.In particular with people
Growth in the living standard and the continuous improvement of diet structure, the social demand of milk be continuously increased, the milk cow according to routine is numerous
Educate development speed, it is difficult to meet the needs of people are growing to milk.How to breed milk cow using new and high technology is unconventional and be
Put an important and pressing task in face of the special herding worker of numerous scientific workers.
China is studied embryo's biotechnology since the seventies, and research and development speed is accelerated after the eighties,
In recent years, gratifying achievement in research is achieved in terms of embryo's bioengineering single technology.Particularly superfecundation, in vitro by
In terms of the engineering technology such as essence, embryo collection, sex identification, embryo separating, embryo cloning, Embryo freezing preservation and embryo transfer,
Have compared with quantum jump and progress, accelerate the breeding of milk cow and the improvement of quality to a certain extent, but in control milk cow birth
It sex technical elements, can not break through, will seriously govern the extraordinary super development speed bred of milk cow.
The content of the invention
It is an object of the invention to provide a kind of using the unconventional method for breeding fine breed cow of embryo biotechnology, the present invention
Realized by following technology：
It is a kind of using the unconventional method for breeding fine breed cow of embryo biotechnology, select outstanding and suitable breast milk Niu Zuowei
Donor, it the described method comprises the following steps：
A, superfecundation：In the preceding 4d of selected female cow oestrus, i.e. the 16th of oestrous cycle day or the 17th day, in muscle or
PMSG1500-3000IU is subcutaneously injected；
B, artificial insemination：After female cow oestrus by superfecundation processing, the seminal fluid of excellent bull is selected, suitable
Time carries out artificial insemination；
C, embryo is rushed：Egg apparatus is rushed using two tunnel formula conduits, flushing liquor is injected in cornua uteri by inner tube, then export is rinsed
Liquid, and required embryo's flushing liquor can be obtained；
D, the inspection of embryo：The flushing liquor that step c is collected into, 10min is stood, after embryo sinks, removes upper liquid,
It is placed on test under microscope quantity and developmental state；
E, embryo cryopreservation：Embryo, which is placed in the Du Shi nutrient solutions containing antifreezing agent, takes slow cooling to freeze；
F, embryo thawing：It can be placed directly in room temperature and thaw, antifreezing agent is disposed in defrosting；
G, embryo separates：Embryo is carried out using micromanipulation instrument more than hemiembryo to split；
H, embryo transfer：Using nonsurgical transfered method, a hand stretches into rectum, checks that corpus luteum is located at which side and development feelings
Condition, cervix is then held, the other hand is by grafts insertion with the cornua uteri of corpus luteum homonymy, injecting embryo.
The PMSG of an above-mentioned amount of intramuscular injection in the step a, the next day inject PGF2a30~40mg again.
In the step b, artificial insemination should use the big seminal fluid of motility rate high density, and insemination time is increased into 2 times or 3
It is secondary, two 8~10h of minor tick.
Flushing liquor described in step c is Du Shi phosphate buffers.
The embryo that rushes typically is carried out in 6~8d of artificial insemination.
The step e, needed before freezing first successively by containing the Du Shi nutrient solutions by low concentration to high concentration antifreezing agent,
The highest gradient of antifreezing agent is 1.0~1.5mol/l, and 5~10min is stopped in every kind of depth, is cooled in a kind of last depth cold
Freeze.
The step e, slow cooling must be taken in freezing, and -5 DEG C~-7 DEG C are down to by room temperature, 1 DEG C of drop per minute,
Induction icing is carried out at this temperature, is then down to -33 DEG C~-38 DEG C by the speed of 0.3 DEG C~0.5 DEG C of drop per minute, hereafter
Liquid nitrogen storage can be directly immersed in.
The step f, the dilution of sucrose liquid is added after defrosting.
The step h, the acceptor of embryo transfer can select ox or other kinds of oxen.
In order to control and improve the birth rate of female calf of cow, it will thus provide the semen deposition of the right testis band positive sperm of bull of seminal fluid
The artificial ligation of pipe, it is ensured that left X sperm of the testis discharge with feminine gender, and the right ovary fallopian tubal of donor cows is ligatured, really
Protect left X ovum of the oviduct discharge with feminine gender.
The beneficial effects of the invention are as follows：The above method is taken, the birth rate of female calf can be efficiently controlled, and is reduced
Production cost, the quality of milk cow and milk is improved, reached the purpose of extraordinary fast breeding breast milk ox.
Embodiment
It is a kind of using the unconventional method for breeding fine breed cow of embryo biotechnology, select outstanding and suitable breast milk Niu Zuowei
Donor, it the described method comprises the following steps：A, superfecundation：The 16th of the preceding 4d, i.e. oestrous cycle of selected female cow oestrus the
It or the 17th day, in muscle or PMSG1500-3000IU is subcutaneously injected；B, artificial insemination：The breast milk handled by superfecundation
After ox heat, the seminal fluid of excellent bull is selected, artificial insemination is carried out in suitable time；C, embryo is rushed：Utilize two tunnel formula conduit punchings
Egg apparatus, flushing liquor is injected in cornua uteri by inner tube, then exports flushing liquor, and required embryo's flushing liquor can be obtained；D, embryo
The inspection of tire：The flushing liquor that step c is collected into, 10min is stood, after embryo sinks, remove upper liquid, put under the microscope
Check quantity and developmental state；E, embryo cryopreservation：Embryo is placed in the Du Shi nutrient solutions containing antifreezing agent and takes slow cooling
Freezing；F, embryo thawing：It can be placed directly in room temperature and thaw, antifreezing agent is disposed in defrosting；G, embryo separates：Utilize micromanipulation
Instrument carries out more than hemiembryo splitting to embryo；H, embryo transfer：Using nonsurgical transfered method, a hand stretches into rectum, checks corpus luteum position
In which side and developmental state, cervix is then held, the other hand is by grafts insertion with the cornua uteri of corpus luteum homonymy, injecting
Embryo.The PMSG of an above-mentioned amount of intramuscular injection in the step a, the next day inject PGF2a30~40mg again；In the step b, manually
Insemination should use the big seminal fluid of motility rate high density, and insemination time be increased to 2 times or 3 times, two 8~10h of minor tick；Step c
Described in flushing liquor be Du Shi phosphate buffers.The embryo that rushes typically is carried out in 6~8d of artificial insemination；The step e, cold
Needed before jelly first successively by containing the Du Shi nutrient solutions by low concentration to high concentration antifreezing agent, the highest gradient of antifreezing agent is 1.0
~1.5mol/l, 5~10min is stopped in every kind of depth, cool freezing in a kind of last depth；The step e, in freezing
Slow cooling must be taken, -5 DEG C~-7 DEG C is down to by room temperature, 1 DEG C of drop per minute, carries out induction icing at this temperature, then
- 33 DEG C~-38 DEG C are down to by the speed of 0.3 DEG C~0.5 DEG C of drop per minute, hereafter can be directly immersed in Liquid nitrogen storage；The step
F, the dilution of sucrose liquid is added after defrosting；The step h, the acceptor of embryo transfer can select ox or other kinds of oxen；In order to control
System and the birth rate for improving female calf of cow, it will thus provide the vas deferens of the right testis band positive sperm of bull of seminal fluid manually ligatures, really
Left X sperm of the testis discharge with feminine gender is protected, and the right ovary fallopian tubal of donor cows is ligatured, it is ensured that left oviduct discharges band
Negative X ovum.
Claims (10)
1. it is a kind of using the unconventional method for breeding fine breed cow of embryo biotechnology, select outstanding and suitable breast milk Niu Zuowei to supply
Body, it is characterised in that：It the described method comprises the following steps：
A, superfecundation：In the preceding 4d of selected female cow oestrus, i.e. the 16th of oestrous cycle day or the 17th day, in muscle or subcutaneous
PMSG Injection 1500-3000IU；
B, artificial insemination：After female cow oestrus by superfecundation processing, the seminal fluid of excellent bull is selected, in suitable time
Carry out artificial insemination；
C, embryo is rushed：Egg apparatus is rushed using two tunnel formula conduits, flushing liquor is injected in cornua uteri by inner tube, then exports flushing liquor,
And embryo's flushing liquor needed for can obtaining；
D, the inspection of embryo：The flushing liquor that step c is collected into, 10min is stood, after embryo sinks, remove upper liquid, be placed on
Test under microscope quantity and developmental state；
E, embryo cryopreservation：Embryo, which is placed in the Du Shi nutrient solutions containing antifreezing agent, takes slow cooling to freeze；
F, embryo thawing：It can be placed directly in room temperature and thaw, antifreezing agent is disposed in defrosting；
G, embryo separates：Embryo is carried out using micromanipulation instrument more than hemiembryo to split；
H, embryo transfer：Using nonsurgical transfered method, a hand stretches into rectum, checks that corpus luteum is located at which side and developmental state, so
After hold cervix, the other hand is by grafts insertion with the cornua uteri of corpus luteum homonymy, injecting embryo.
2. a kind of according to claim 1 existed using the unconventional method for breeding fine breed cow of embryo biotechnology, its feature
In：The PMSG of an above-mentioned amount of intramuscular injection in the step a, the next day inject PGF2a30~40mg again.
3. a kind of according to claim 1 existed using the unconventional method for breeding fine breed cow of embryo biotechnology, its feature
In：In the step b, artificial insemination should use the big seminal fluid of motility rate high density, and insemination time increased into 2 times or 3 times, and two
8~10h of minor tick.
4. a kind of according to claim 1 existed using the unconventional method for breeding fine breed cow of embryo biotechnology, its feature
In：Flushing liquor described in step c is Du Shi phosphate buffers.
5. a kind of according to claim 1 existed using the unconventional method for breeding fine breed cow of embryo biotechnology, its feature
In：The embryo that rushes typically is carried out in 6~8d of artificial insemination.
6. a kind of according to claim 1 existed using the unconventional method for breeding fine breed cow of embryo biotechnology, its feature
In：The step e, needed before freezing first successively by containing the Du Shi nutrient solutions by low concentration to high concentration antifreezing agent, it is antifreeze
The highest gradient of agent is 1.0~1.5mol/l, and 5~10min is stopped in every kind of depth, and cool freezing in a kind of last depth.
7. a kind of according to claim 1 existed using the unconventional method for breeding fine breed cow of embryo biotechnology, its feature
In：The step e, slow cooling must be taken in freezing, be down to -5 DEG C~-7 DEG C by room temperature, 1 DEG C of drop per minute is warm herein
Induction icing is carried out under degree, is then down to -33 DEG C~-38 DEG C by the speed of 0.3 DEG C~0.5 DEG C of drop per minute, hereafter can be direct
Immerse Liquid nitrogen storage.
8. a kind of according to claim 1 existed using the unconventional method for breeding fine breed cow of embryo biotechnology, its feature
In：The step f, the dilution of sucrose liquid is added after defrosting.
9. a kind of according to claim 1 existed using the unconventional method for breeding fine breed cow of embryo biotechnology, its feature
In：The step h, the acceptor of embryo transfer can select ox or other kinds of oxen.
10. a kind of according to claim 1 existed using the unconventional method for breeding fine breed cow of embryo biotechnology, its feature
In：In order to control and improve the birth rate of female calf of cow, it will thus provide the vas deferens people of the right testis band positive sperm of bull of seminal fluid
Work ligatures, it is ensured that left X sperm of the testis discharge with feminine gender, and the right ovary fallopian tubal of donor cows is ligatured, it is ensured that it is left
X ovum of the oviduct discharge with feminine gender.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710419760.XA CN107397608A (en) | 2017-06-06 | 2017-06-06 | It is a kind of using the unconventional method for breeding fine breed cow of embryo biotechnology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710419760.XA CN107397608A (en) | 2017-06-06 | 2017-06-06 | It is a kind of using the unconventional method for breeding fine breed cow of embryo biotechnology |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107397608A true CN107397608A (en) | 2017-11-28 |
Family
ID=60405095
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710419760.XA Pending CN107397608A (en) | 2017-06-06 | 2017-06-06 | It is a kind of using the unconventional method for breeding fine breed cow of embryo biotechnology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107397608A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109364240A (en) * | 2018-08-30 | 2019-02-22 | 宁波三生生物科技有限公司 | A kind of pregnant mare serum gonadotrop(h)in (PMSG) freeze-dried powder and preparation method thereof |
| CN110063812A (en) * | 2018-01-24 | 2019-07-30 | 中国农业大学 | A method of improving jenny reproductive performance in timing insemination technique |
-
2017
- 2017-06-06 CN CN201710419760.XA patent/CN107397608A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110063812A (en) * | 2018-01-24 | 2019-07-30 | 中国农业大学 | A method of improving jenny reproductive performance in timing insemination technique |
| CN109364240A (en) * | 2018-08-30 | 2019-02-22 | 宁波三生生物科技有限公司 | A kind of pregnant mare serum gonadotrop(h)in (PMSG) freeze-dried powder and preparation method thereof |
| CN109364240B (en) * | 2018-08-30 | 2021-09-21 | 宁波三生生物科技股份有限公司 | Pregnant mare serum gonadotropin freeze-dried powder and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Hansen et al. | Adverse impact of heat stress on embryo production: causes and strategies for mitigation | |
| Perera | Reproduction in domestic buffalo | |
| Drost | Advanced reproductive technology in the water buffalo | |
| Dochi et al. | The use of embryo transfer to produce pregnancies in repeat-breeding dairy cattle | |
| Aller et al. | Successful transfer of vitrified Ilama (Lama glama) embryos | |
| CN103184189A (en) | Cultivation method of cross-bred wagy | |
| Skidmore | The use of some assisted reproductive technologies in old world camelids | |
| Squires et al. | Cryopreservation of equine embryos | |
| Parks | Processing and handling bull semen for artificial insemination—Don't add insult to injury | |
| CN107397608A (en) | It is a kind of using the unconventional method for breeding fine breed cow of embryo biotechnology | |
| CN103891711B (en) | The processing method of flower perch seminal fluid | |
| Bui-Xuan-Nguyen et al. | Direct freezing of cattle embryos after partial dehydration at room temperature | |
| Park et al. | Improved pregnancy rate and sex ratio in fresh/frozen in vivo derived embryo transfer of Hanwoo (Bos taurus coreanae) cows | |
| Almeida et al. | Use of cooled buffalo semen as a strategy to increase conception rates in fixed-time artificial insemination programs during unfavorable reproductive periods | |
| Perry et al. | Management factors influencing fertility in beef cattle breeding programmes | |
| Skidmore et al. | Reproductive technologies in camelids | |
| CN104042355A (en) | Method for unconventionally breeding improved cows through embryo biotechnology | |
| Banker et al. | The impact of vitrification in artificial reproductive technology programmes | |
| Sánchez‐Sánchez et al. | Reproductive efficiency of sows inseminated at single dose fixed time with refrigerated, cryopreserved and encapsulated spermatozoa | |
| Bratton et al. | Fertility of bovine spermatozoa stored at minus 79 C. For one week and for seventeen weeks | |
| Demeterco et al. | The effects of intramuscular or intravenous injections of gonadotropin releasing hormone at fixed-time artificial insemination on pregnancy rates of Bos indicus beef cows | |
| CN1226377A (en) | Process for industrializing technology of 'tube cattle' | |
| Skidmore et al. | Comparison of two closed vitrification methods for vitrifying dromedary camel (Camelus dromedarius) embryos | |
| Říha et al. | Viability of fresh and frozen transferred IVP bovine embryos | |
| Heo et al. | Development of a method of vitrification, thawing, and transfer of mammalian blastocysts using a single closed cryo-straw |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20171128 |
|
| WD01 | Invention patent application deemed withdrawn after publication |