CN107397226A - A kind of reducing blood lipid sea cucumber polypeptide microcapsules and preparation method thereof - Google Patents
A kind of reducing blood lipid sea cucumber polypeptide microcapsules and preparation method thereof Download PDFInfo
- Publication number
- CN107397226A CN107397226A CN201710524864.7A CN201710524864A CN107397226A CN 107397226 A CN107397226 A CN 107397226A CN 201710524864 A CN201710524864 A CN 201710524864A CN 107397226 A CN107397226 A CN 107397226A
- Authority
- CN
- China
- Prior art keywords
- sea cucumber
- microcapsules
- parts
- polypeptide
- reducing blood
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000251511 Holothuroidea Species 0.000 title claims abstract description 128
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 57
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 57
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 57
- 239000003094 microcapsule Substances 0.000 title claims abstract description 50
- 239000008280 blood Substances 0.000 title claims abstract description 25
- 210000004369 blood Anatomy 0.000 title claims abstract description 25
- 150000002632 lipids Chemical class 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 150000004676 glycans Chemical class 0.000 claims abstract description 33
- 239000005017 polysaccharide Substances 0.000 claims abstract description 33
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 31
- 239000000839 emulsion Substances 0.000 claims abstract description 26
- 108010010803 Gelatin Proteins 0.000 claims abstract description 19
- 229920000159 gelatin Polymers 0.000 claims abstract description 19
- 239000008273 gelatin Substances 0.000 claims abstract description 19
- 235000019322 gelatine Nutrition 0.000 claims abstract description 19
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 19
- 239000002994 raw material Substances 0.000 claims abstract description 12
- 239000000284 extract Substances 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 28
- 235000019441 ethanol Nutrition 0.000 claims description 16
- 239000006228 supernatant Substances 0.000 claims description 16
- 239000012141 concentrate Substances 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 238000005119 centrifugation Methods 0.000 claims description 8
- 238000004108 freeze drying Methods 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 238000001556 precipitation Methods 0.000 claims description 8
- 238000001976 enzyme digestion Methods 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 230000033228 biological regulation Effects 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 239000012153 distilled water Substances 0.000 claims description 4
- 238000000605 extraction Methods 0.000 claims description 4
- 239000000796 flavoring agent Substances 0.000 claims description 4
- 235000019634 flavors Nutrition 0.000 claims description 4
- 238000000108 ultra-filtration Methods 0.000 claims description 4
- 210000001835 viscera Anatomy 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 108091005804 Peptidases Proteins 0.000 claims description 3
- 239000004365 Protease Substances 0.000 claims description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 239000003643 water by type Substances 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 6
- 210000003734 kidney Anatomy 0.000 abstract description 3
- 241000965254 Apostichopus japonicus Species 0.000 abstract description 2
- 229920001231 Polysaccharide peptide Polymers 0.000 abstract description 2
- 230000036039 immunity Effects 0.000 abstract description 2
- 108010022457 polysaccharide peptide Proteins 0.000 abstract description 2
- 239000000047 product Substances 0.000 description 8
- 239000002775 capsule Substances 0.000 description 5
- 239000003292 glue Substances 0.000 description 4
- 241000208340 Araliaceae Species 0.000 description 2
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 2
- 235000003140 Panax quinquefolius Nutrition 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 235000008434 ginseng Nutrition 0.000 description 2
- 101000984728 Chiropsoides quadrigatus Angiotensin-converting enzyme inhibitory peptide Proteins 0.000 description 1
- 241000251730 Chondrichthyes Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 244000000626 Daucus carota Species 0.000 description 1
- 241000258955 Echinodermata Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 208000034189 Sclerosis Diseases 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000005770 birds nest Nutrition 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 239000007937 lozenge Substances 0.000 description 1
- 238000003754 machining Methods 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 235000005765 wild carrot Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
A kind of reducing blood lipid sea cucumber polypeptide microcapsules and preparation method thereof, belong to health products processing technique field.The microcapsules are made up according to mass fraction of 23 parts of gelatin, 46 parts of sea cucumber polysaccharides, 29 parts of sea cucumber polypeptides.Preparation process is as follows：（1）Sea cucumber polysaccharide extracts；（2）Sea cucumber polypeptide extracts；（3）It is prepared by microcapsules emulsion；（4）Microcapsules emulsion is spray-dried.It is an advantage of the invention that：This project prepares the sea cucumber polypeptide microcapsules with lipid-lowering effect using wall of sea cucumber Stichopus japonicus as raw material.This method can more make full use of the main active polysaccharide and polypeptide of sea cucumber, both is incorporated in same product, remain the original active component of sea cucumber to greatest extent, given play to sea cucumber health care effect.Product storage is easy to carry, active component is stable and effective control release, not only with single-minded lipid-lowering effect, moreover it is possible to physical fitness, enriching yin and nourishing kidney, strengthen immunity.
Description
Technical field
The invention belongs to health products processing technique field, and in particular to a kind of reducing blood lipid sea cucumber polypeptide microcapsules and its preparation
Method.
Background technology
Sea cucumber, belong to Holothuroidea, be the ocean echinoderm to 8000 meters by the sea that lives, gone through away from modern existing more than 600,000,000 years
History, sea cucumber is using seabed algae and planktonic organism as food.Sea cucumber whole body covers with corn, is widely distributed in each ocean in the world.China South Sea
Littoral species is more, and there are about more than 20 kind sea cucumbers can be edible, and the same ginseng of sea cucumber, bird's nest, shark's fin are equally celebrated for their achievements, are eight big treasures of the world
One of.Sea cucumber is not only the food of preciousness, and rare medicinal material.According to《A Supplement to the Compendium of Materia Medica》Described in：Sea cucumber, sweet-salty,
Kidney tonifying, benefiting quintessence take the photograph urine, and impotence, its warm-natured benefit, sufficient enemy's ginseng, therefore named sea cucumber are treated in establishing-Yang.Sea cucumber, which has, improves memory, prolongs
Slow sexual gland aging, prevent artery sclerosis and antitumor wait from acting on.With the popularization of sea cucumber value knowledge, sea cucumber progresses into hundred
Surname dining table.Living environment determines sea cucumber quality.
Domestic beche-de-mer products mainly have sea cucumber fresh goods, traditional sea cucumber dry product, the sea cucumber dry product of deep processing.Traditional Holothurian machining
Many shortcomings be present in method：First, nutriment loss is too big, second, inconvenient for use, water hair time length, third, easily being filled with secondary
It is good.
At present, the thousands of items of sea cucumber health care product patent, sea cucumber polypeptide mainly prepares, prepared by sea cucumber ace inhibitory peptide, sea cucumber is more
Sugar preparation etc., object form has capsule, oral liquid and lozenge etc..Reducing blood lipid sea cucumber polypeptide microcapsules are not found.
The content of the invention
The invention aims to solve the problems, such as at present without reducing blood lipid sea cucumber polypeptide microencapsulated product, there is provided one kind drop
Blood fat sea cucumber polypeptide microcapsules and preparation method thereof.
To achieve the above object, the technical scheme that the present invention takes is as follows：
A kind of reducing blood lipid sea cucumber polypeptide microcapsules, the raw material of the microcapsules are extra large by 2-3 parts gelatin, 4-6 parts according to mass fraction
Gracilis polysaccharide, 2-9 parts sea cucumber polypeptide composition.
A kind of preparation method of above-mentioned reducing blood lipid sea cucumber polypeptide microcapsules, methods described step are as follows：
Step 1：Sea cucumber polysaccharide extracts
（1）Clean, rub：Sea cucumber is removed the internal organs, is cleaned and is rubbed and freeze standby；
（2）Enzymolysis：10-15g is taken to freeze sea cucumber, by solid-liquid ratio 1g:10-20mL adds distilled water, adds by the 1-3% of quality of sea cucumber
Enter flavor protease, regulation pH value is to neutrality, the water enzyme digestion 4-7h at 40-55 DEG C, takes out afterwards whole in 90 DEG C of min of water-bath 20
Only enzyme digestion reaction；
（3）Ultrasonic wave：Sea cucumber after enzymolysis is ultrasonically treated, processing power 200-300W, processing time 20-
30min, then with 9000r/min centrifugation 20min, supernatant is taken after centrifugation, discards precipitation；
（4）Concentration：By the 1/6 of supernatant concentration to original volume, concentrate is obtained；
（5）Alcohol is analysed：To（4）95% ethanol is added in obtained concentrate, stirring is sufficiently mixed, makes concentration of alcohol in concentrate
Reach 80%, 4 DEG C of left undisturbed overnights, mixed liquor is centrifuged afterwards, removes supernatant, is precipitated；
（6）Washing, dry：Precipitation is washed with absolute ethyl alcohol, sea cucumber polysaccharide is produced after freeze-drying；
Step 2：Sea cucumber polypeptide extracts
The supernatant that removes during alcohol analysis in the sea cucumber polysaccharide extraction of collection step one, successively with molecule interception 3kDa and
600Da milipore filter carries out ultrafiltration, and collection arrives 600Da-3kDa components, and freeze-drying obtains sea cucumber polypeptide powder；
Step 3：It is prepared by microcapsules emulsion
Using gelatin and sea cucumber polysaccharide as wall material, sea cucumber polypeptide is core；Weigh 2-3 parts gelatin and 4-6 parts sea cucumber polysaccharide it is molten
Enter in 100 parts of deionized waters, stir, make to be completely dissolved, be made into mixed liquor；It is 1 by core wall mass ratio：1~3 adds pretreatment
Good sea cucumber polypeptide powder, stable emulsion is formed after stirring 10~30 min, by emulsion under 30~50 MPa pressure
Homogeneous 1~3 time, form microcapsules emulsion；
Step 4：Microcapsules emulsion is spray-dried
Microcapsules emulsion is with the mL/h of charging rate 700~900, and 50~70 DEG C of inlet temperature, 3~8bar of pressure is sprayed
Dry, obtain reducing blood lipid sea cucumber polypeptide microcapsules.
It is of the invention to be relative to the beneficial effect of prior art：For the present invention using wall of sea cucumber Stichopus japonicus as raw material, preparing has drop blood
The sea cucumber polypeptide microcapsules of fat effect.This method can more make full use of the main active polysaccharide and polypeptide of sea cucumber, make both
It is incorporated in same product, remains the original active component of sea cucumber to greatest extent, given play to sea cucumber health care effect.The product is deposited
Easy to carry, active component stabilization and effective control release are stored up, not only with single-minded lipid-lowering effect, moreover it is possible to physical fitness,
Enriching yin and nourishing kidney, strengthen immunity.
Embodiment
The technical scheme of invention is further illustrated with reference to embodiment, but office is not only limitted to this, it is every to this hair
Bright technical scheme is modified or equivalent substitution, without departing from technical solution of the present invention scope, all should cover in the present invention
Protection domain among.
Embodiment one：What present embodiment was recorded is a kind of reducing blood lipid sea cucumber polypeptide microcapsules, the microcapsules
Raw material be made up of according to mass fraction 2-3 parts gelatin, 4-6 parts sea cucumber polysaccharide, 2-9 part sea cucumber polypeptides.
Embodiment two：A kind of reducing blood lipid sea cucumber polypeptide microcapsules described in embodiment one, micro- glue
The raw material of capsule is made up of according to mass fraction 2 parts of gelatin, 4 parts of sea cucumber polysaccharides, 2 parts of sea cucumber polypeptides.
Embodiment three：A kind of reducing blood lipid sea cucumber polypeptide microcapsules described in embodiment one, micro- glue
The raw material of capsule is made up of according to mass fraction 3 parts of gelatin, 6 parts of sea cucumber polysaccharides, 9 parts of sea cucumber polypeptides.
Embodiment four：A kind of reducing blood lipid sea cucumber polypeptide microcapsules described in embodiment one, micro- glue
The raw material of capsule is made up of according to mass fraction 2.5 parts of gelatin, 5 parts of sea cucumber polysaccharides, 5 parts of sea cucumber polypeptides.
Embodiment five：A kind of preparation side of reducing blood lipid sea cucumber polypeptide microcapsules described in embodiment one
Method, methods described step are as follows：
Step 1：Sea cucumber polysaccharide extracts
（1）Clean, rub：Sea cucumber is removed the internal organs, is cleaned and is rubbed and freeze standby；
（2）Enzymolysis：10-15g is taken to freeze sea cucumber, by solid-liquid ratio 1g:10-20mL adds distilled water, adds by the 1-3% of quality of sea cucumber
Enter flavor protease, regulation pH value is to neutrality, the water enzyme digestion 4-7h at 40-55 DEG C, takes out afterwards whole in 90 DEG C of min of water-bath 20
Only enzyme digestion reaction；
（3）Ultrasonic wave：Sea cucumber after enzymolysis is ultrasonically treated, processing power 200-300W, processing time 20-
30min, then with 9000r/min centrifugation 20min, supernatant is taken after centrifugation, discards precipitation；
（4）Concentration：By the 1/6 of supernatant concentration to original volume, concentrate is obtained；
（5）Alcohol is analysed：To（4）95% ethanol is added in obtained concentrate, stirring is sufficiently mixed, makes concentration of alcohol in concentrate
Reach 80%, 4 DEG C of left undisturbed overnights, mixed liquor is centrifuged afterwards, removes supernatant, is precipitated；
（6）Washing, dry：Precipitation is washed with absolute ethyl alcohol, sea cucumber polysaccharide is produced after freeze-drying；
Step 2：Sea cucumber polypeptide extracts
The supernatant that removes during alcohol analysis in the sea cucumber polysaccharide extraction of collection step one, successively with molecule interception 3kDa and
600Da milipore filter carries out ultrafiltration, and collection arrives 600Da-3kDa components, and freeze-drying obtains sea cucumber polypeptide powder；
Step 3：It is prepared by microcapsules emulsion
Using gelatin and sea cucumber polysaccharide as wall material, sea cucumber polypeptide is core；Weigh 2-3 parts gelatin and 4-6 parts sea cucumber polysaccharide it is molten
Enter in 100 parts of deionized waters, stir, make to be completely dissolved, be made into mixed liquor；It is 1 by core wall mass ratio：1~3 adds pretreatment
Good sea cucumber polypeptide powder, stable emulsion is formed after stirring 10~30 min, by emulsion under 30~50 MPa pressure
Homogeneous 1~3 time, form microcapsules emulsion；
Step 4：Microcapsules emulsion is spray-dried
Microcapsules emulsion is with the mL/h of charging rate 700~900, and 50~70 DEG C of inlet temperature, 3~8bar of pressure is sprayed
Dry, obtain reducing blood lipid sea cucumber polypeptide microcapsules.
Embodiment 1：
A kind of reducing blood lipid sea cucumber polypeptide microcapsules, the raw material of the microcapsules are more by 2 parts of gelatin, 4 portions of sea cucumbers according to mass fraction
Sugar, 2 parts of sea cucumber polypeptide compositions.Specific preparation method is as follows：
Step 1：Sea cucumber polysaccharide extracts
（1）Clean, rub：Sea cucumber is removed the internal organs, is cleaned and is rubbed and freeze standby；
（2）Enzymolysis：10g is taken to freeze sea cucumber, by solid-liquid ratio 1g:10mL adds distilled water, and flavor egg is added by the 1% of quality of sea cucumber
White enzyme, regulation pH value the water enzyme digestion 4h at 40 DEG C, are taken out in 90 DEG C of min enzymolysis reactions of water-bath 20 afterwards to neutrality；
（3）Ultrasonic wave：Sea cucumber after enzymolysis is ultrasonically treated, processing power 200W, processing time 20min, then
With 9000r/min centrifugation 20min, supernatant is taken after centrifugation, discards precipitation；
（4）Concentration：By the 1/6 of supernatant concentration to original volume, concentrate is obtained；
（5）Alcohol is analysed：To（4）95% ethanol is added in obtained concentrate, stirring is sufficiently mixed, makes concentration of alcohol in concentrate
Reach 80%, 4 DEG C of left undisturbed overnights, mixed liquor is centrifuged afterwards, removes supernatant, is precipitated；
（6）Washing, dry：Precipitation is washed with absolute ethyl alcohol, sea cucumber polysaccharide is produced after freeze-drying；
Step 2：Sea cucumber polypeptide extracts
The supernatant that removes during alcohol analysis in the sea cucumber polysaccharide extraction of collection step one, successively with molecule interception 3kDa and
600Da milipore filter carries out ultrafiltration, and collection arrives 600Da-3kDa components, and freeze-drying obtains sea cucumber polypeptide powder；
Step 3：It is prepared by microcapsules emulsion
Using gelatin and sea cucumber polysaccharide as wall material, sea cucumber polypeptide is core；The gelatin and 4 parts of sea cucumber polysaccharide for weighing 2 parts dissolve in 100
In part deionized water, stirring, make to be completely dissolved, be made into mixed liquor；It is 1 by core wall mass ratio：3 add the sea cucumber pre-processed
Polypeptide powder, stable emulsion is formed after stirring 10 min, homogeneous 3 times under 30 MPa pressure, form micro- glue by emulsion
Capsule emulsion；
Step 4：Microcapsules emulsion is spray-dried
With the mL/h of charging rate 700,60 DEG C of inlet temperature, pressure 5bar is spray-dried microcapsules emulsion, obtains dropping blood
Fat sea cucumber polypeptide microcapsules.
Claims (5)
- A kind of 1. reducing blood lipid sea cucumber polypeptide microcapsules, it is characterised in that：The raw material of the microcapsules is according to mass fraction by 2-3 parts Gelatin, 4-6 parts sea cucumber polysaccharide, 2-9 parts sea cucumber polypeptide composition.
- A kind of 2. reducing blood lipid sea cucumber polypeptide microcapsules according to claim 1, it is characterised in that：The raw material of the microcapsules It is made up of according to mass fraction 2 parts of gelatin, 4 parts of sea cucumber polysaccharides, 2 parts of sea cucumber polypeptides.
- A kind of 3. reducing blood lipid sea cucumber polypeptide microcapsules according to claim 1, it is characterised in that：The raw material of the microcapsules It is made up of according to mass fraction 3 parts of gelatin, 6 parts of sea cucumber polysaccharides, 9 parts of sea cucumber polypeptides.
- A kind of 4. reducing blood lipid sea cucumber polypeptide microcapsules according to claim 1, it is characterised in that：The raw material of the microcapsules It is made up of according to mass fraction 2.5 parts of gelatin, 5 parts of sea cucumber polysaccharides, 5 parts of sea cucumber polypeptides.
- A kind of 5. preparation method of the reducing blood lipid sea cucumber polypeptide microcapsules described in claim 1, it is characterised in that：Methods described walks It is rapid as follows：Step 1：Sea cucumber polysaccharide extracts（1）Clean, rub：Sea cucumber is removed the internal organs, is cleaned and is rubbed and freeze standby；（2）Enzymolysis：10-15g is taken to freeze sea cucumber, by solid-liquid ratio 1g:10-20mL adds distilled water, adds by the 1-3% of quality of sea cucumber Enter flavor protease, regulation pH value is to neutrality, the water enzyme digestion 4-7h at 40-55 DEG C, takes out afterwards whole in 90 DEG C of min of water-bath 20 Only enzyme digestion reaction；（3）Ultrasonic wave：Sea cucumber after enzymolysis is ultrasonically treated, processing power 200-300W, processing time 20- 30min, then with 9000r/min centrifugation 20min, supernatant is taken after centrifugation, discards precipitation；（4）Concentration：By the 1/6 of supernatant concentration to original volume, concentrate is obtained；（5）Alcohol is analysed：To（4）95% ethanol is added in obtained concentrate, stirring is sufficiently mixed, makes concentration of alcohol in concentrate Reach 80%, 4 DEG C of left undisturbed overnights, mixed liquor is centrifuged afterwards, removes supernatant, is precipitated；（6）Washing, dry：Precipitation is washed with absolute ethyl alcohol, sea cucumber polysaccharide is produced after freeze-drying；Step 2：Sea cucumber polypeptide extractsThe supernatant that removes during alcohol analysis in the sea cucumber polysaccharide extraction of collection step one, successively with molecule interception 3kDa and 600Da milipore filter carries out ultrafiltration, and collection arrives 600Da-3kDa components, and freeze-drying obtains sea cucumber polypeptide powder；Step 3：It is prepared by microcapsules emulsionUsing gelatin and sea cucumber polysaccharide as wall material, sea cucumber polypeptide is core；Weigh 2-3 parts gelatin and 4-6 parts sea cucumber polysaccharide it is molten Enter in 100 parts of deionized waters, stir, make to be completely dissolved, be made into mixed liquor；It is 1 by core wall mass ratio：1~3 adds pretreatment Good sea cucumber polypeptide powder, stable emulsion is formed after stirring 10~30 min, by emulsion under 30~50 MPa pressure Homogeneous 1~3 time, form microcapsules emulsion；Step 4：Microcapsules emulsion is spray-driedMicrocapsules emulsion is with the mL/h of charging rate 700~900, and 50~70 DEG C of inlet temperature, 3~8bar of pressure is sprayed Dry, obtain reducing blood lipid sea cucumber polypeptide microcapsules.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710524864.7A CN107397226A (en) | 2017-06-30 | 2017-06-30 | A kind of reducing blood lipid sea cucumber polypeptide microcapsules and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710524864.7A CN107397226A (en) | 2017-06-30 | 2017-06-30 | A kind of reducing blood lipid sea cucumber polypeptide microcapsules and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107397226A true CN107397226A (en) | 2017-11-28 |
Family
ID=60404617
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710524864.7A Pending CN107397226A (en) | 2017-06-30 | 2017-06-30 | A kind of reducing blood lipid sea cucumber polypeptide microcapsules and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107397226A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108576371A (en) * | 2018-03-30 | 2018-09-28 | 大连工业大学 | A kind of microcapsule embedded double protein peptide and preparation method thereof |
| CN115606811A (en) * | 2022-04-19 | 2023-01-17 | 中国科学院烟台海岸带研究所 | Preparation method of stichopus japonicus visceral oligopeptide microcapsules and microcapsules |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101191139A (en) * | 2007-04-28 | 2008-06-04 | 付学军 | Integrated extraction technique for sea cucumber polypeptide and polysaccharide |
| CN101676297A (en) * | 2008-09-19 | 2010-03-24 | 大连正大海洋生物有限公司 | Preparation method of sea cucumber active polypeptide under ultrasonic extraction |
| CN103937860A (en) * | 2014-03-26 | 2014-07-23 | 刘凯 | Preparation method for sea cucumber polypeptide for promoting postoperation wound healing |
| CN104946713A (en) * | 2015-06-29 | 2015-09-30 | 姜乃义 | Preparation method and application of glucose-lowering lipid-lowering peptide based on sea cucumber protein |
| CN105524967A (en) * | 2016-03-02 | 2016-04-27 | 集美大学 | Sea cucumber polysaccharide and sea cucumber collagen polypeptide combined preparation method |
-
2017
- 2017-06-30 CN CN201710524864.7A patent/CN107397226A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101191139A (en) * | 2007-04-28 | 2008-06-04 | 付学军 | Integrated extraction technique for sea cucumber polypeptide and polysaccharide |
| CN101676297A (en) * | 2008-09-19 | 2010-03-24 | 大连正大海洋生物有限公司 | Preparation method of sea cucumber active polypeptide under ultrasonic extraction |
| CN103937860A (en) * | 2014-03-26 | 2014-07-23 | 刘凯 | Preparation method for sea cucumber polypeptide for promoting postoperation wound healing |
| CN104946713A (en) * | 2015-06-29 | 2015-09-30 | 姜乃义 | Preparation method and application of glucose-lowering lipid-lowering peptide based on sea cucumber protein |
| CN105524967A (en) * | 2016-03-02 | 2016-04-27 | 集美大学 | Sea cucumber polysaccharide and sea cucumber collagen polypeptide combined preparation method |
Non-Patent Citations (2)
| Title |
|---|
| 冯玉萍: "《全球海参经济品种图鉴》", 31 December 2013 * |
| 王洪涛: "海参肽的酶法制备工艺及抗疲劳活性研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108576371A (en) * | 2018-03-30 | 2018-09-28 | 大连工业大学 | A kind of microcapsule embedded double protein peptide and preparation method thereof |
| CN115606811A (en) * | 2022-04-19 | 2023-01-17 | 中国科学院烟台海岸带研究所 | Preparation method of stichopus japonicus visceral oligopeptide microcapsules and microcapsules |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103141895B (en) | Natural marine animal peptide beautifying drink | |
| CN102885322B (en) | Functional duck meat square leg and preparation method thereof | |
| CN106954770A (en) | A kind of beauty-care beverage | |
| CN102559443A (en) | Heath-function sea cucumber yellow wine | |
| CN104082819B (en) | A kind of edible and medical fungi functional solid beverage and preparation method thereof | |
| CN103859368A (en) | Sea cucumber beverage and production method thereof | |
| CN101928744B (en) | Process for extracting active collagen peptide from salmon trout waste | |
| CN108935912B (en) | Fish meat protein peptide with DPP-IV inhibition and anti-fatigue functions and preparation method thereof | |
| CN107475341A (en) | A kind of preparation method of extra large intestines active peptide | |
| CN107397226A (en) | A kind of reducing blood lipid sea cucumber polypeptide microcapsules and preparation method thereof | |
| CN103462069B (en) | One keeps fit strong osteotrophy sausage and preparation method thereof | |
| CN102578521A (en) | Scallop skirt seafood sauce and preparation method thereof | |
| CN101935608B (en) | Sea cucumber nutritional health wine and preparation method thereof | |
| CN101982113A (en) | Preparation method of crassostrea gigas meat antifatigue nutrient solution | |
| CN106913857A (en) | A kind of extra large stichopus japonicus active peptide and preparation method thereof | |
| CN102273633A (en) | Method for preparing oyster shell peptide oral liquid | |
| CN102987405B (en) | Method for preparing health food capsules by using cordyceps militaris and cocoon extracts | |
| CN102987408B (en) | Method for extracting nutritional ingredients such as free amino acids from waste cordyceps militaris cocoons | |
| CN101779791A (en) | Composition with health care functions and preparation method thereof | |
| CN104522738A (en) | Seafood flavor black fungus and fish meat sausage and production method thereof | |
| CN108094839A (en) | A kind of method that Corbicula fluminea meat enzymolysis liquid deodorant takes off bitter flavouring | |
| CN103937858A (en) | Method for preparing antioxidant by using schizochytrium limacinum algal meal | |
| CN103622063B (en) | The preparation method of hippocampus ginseng chicken special efficacy herbal cuisine soup | |
| CN106636267A (en) | Extracting method of small-molecular sea cucumber-oyster polypeptide | |
| CN113826907A (en) | A method for preparing solid beverage containing Stichopus japonicus peptide, intestinum Stichopi Japonici peptide and Concha Ostreae peptide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20171128 |