CN107381838A - A kind of preparation method of compound microbial water purification catridge material - Google Patents
A kind of preparation method of compound microbial water purification catridge material Download PDFInfo
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- CN107381838A CN107381838A CN201710774328.2A CN201710774328A CN107381838A CN 107381838 A CN107381838 A CN 107381838A CN 201710774328 A CN201710774328 A CN 201710774328A CN 107381838 A CN107381838 A CN 107381838A
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- water purification
- preparation
- purification catridge
- compound microbial
- water
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
Abstract
The invention discloses a kind of preparation method of compound microbial water purification catridge material, belong to water-purifying material preparing technical field.Aqueous suspension ofkaolin and calcium chloride solution mixing are added in test tube, adds distilled water to stand, obtains zymotic fluid；Above-mentioned zymotic fluid adds the triangular flask for having culture medium, is cultivated under centrifugal condition；After culture, take zymotic fluid to be centrifuged, filter, take the clear liquid of above-mentioned centrifugate in calcium chloride solution in beaker, use Na2HPO3‑NaH2PO3Stirring, stand the zymotic fluid after being centrifuged；Above-mentioned zymotic fluid is taken out, ethanol is added, pelleting centrifugation, removes supernatant, precipitated；Centrifuged with above-mentioned equal rotating speed after washing precipitation with saturation phosphate buffer solution, then precipitated with distillation water washing, dried, obtained dry block is clayed into power to obtain microbial flocculant；Take palygorskite to be calcined under high temperature furnace and obtain palygorskite clay；Microbial flocculant and palygorskite clay mix, and compressor compresses obtain compound microbial water purification catridge material into block filter core.
Description
Technical field
The invention discloses a kind of preparation method of compound microbial water purification catridge material, belongs to water-purifying material technology neck
Domain.
Background technology
With the horizontal raising of people's social life, along be various pernicious gases discharge.One line of many at present
City ceaselessly builds a house, and a lot of residents have bought car, and the pernicious gas of discharge is easy to pollute water quality.Also some Industry Wastes
Water animal excrements heavy metal filterable virus bacterium chemicals agricultural chemicals has the chloroform of carcinogen, also
There is excessive hardness to cause kidney stone.Present people, which live, to be improved constantly, and the requirement to water also improves constantly.Therefore, to change
The problem of philanthropists are high to water quality requirement, the natural living environment of high quality is built, safeguard balance of city ecosystem, cleaner ring
The product of guarantor --- water purifier arises at the historic moment, and is rapidly developed.
On family expenses or small-sized water purifier, scavenging material is commonly stored in filter core, and raw water is filtered into be net by filter core
Water, but when the scavenging material service life in filter core reaches capacity and need to changed, filter core dismounting trouble, and filter core generally couples
Water inlet pipe and water outlet pipe, the water that can cause during dismounting to store in water pipe flow out, and influence the use of water purifier.Also powder is added in oriented raw water
Sorbing material purifies the water purifier of raw water, but without prompt facility is changed, easily causes scavenging material excessively to use, influence water purification
The effluent quality of device.
Therefore, a kind of water purification catridge material with good purifying water effect is invented to technical field of water purification with actively
Meaning.
The content of the invention
Present invention mainly solves technical problem, for being polluted after common water purifier material for core auto-degradation at present
Greatly, a kind of the defects of filter core service life is shorter, there is provided preparation method of compound microbial water purification catridge material.
In order to solve the above-mentioned technical problem, the technical solution adopted in the present invention is：
（1）3~5mL aqueous suspension ofkaolins are added in test tube, 1~3mL calcium chloride solutions, mixing, add 40~45mL of distilled water
Until pH value reaches certain value, stand, obtain substrate；
（2）Above-mentioned substrate is added in the triangular flask equipped with 2~3L sabouraud's agars, sealing and standing culture, production must be cultivated
Thing, by cultured products centrifuge with rotating speed centrifugal treating, isolated upper liquid, take above-mentioned upper liquid to be added to 250mL
Mass fraction is in 1% calcium chloride solution, uses Na2HPO3-NaH2PO3Cushioning liquid adjust pH value, with glass bar stirring 3~
5min, then 4~6min is stood, obtain pre-processing upper liquid；
（3）The above-mentioned pretreatment upper liquids of 50~60mL are taken out, absolute ethyl alcohol is mixed with pretreatment upper liquid, staticly settled, filters,
Filtrate is removed, is precipitated；
（4）Na is used respectively2HPO3-NaH2PO3Cushioning liquid and distilled water wash precipitation 3~5 times successively, and will be heavy after washing
Shallow lake naturally dry, and standard screen is crossed after grinding 30~40min, it is microbial flocculant to obtain sieving powder；
（5）Take 600~800g palygorskites to be put into high temperature furnace, be heated to certain temperature, insulation roasting obtains palygorskite and glued
Soil；
（6）Microbial flocculant and palygorskite clay are mixed, are pressed into block filter core with compressor, you can obtain compound micro-
Biological water purification material for core.
Step（1）Described in aqueous suspension ofkaolin concentration be 5.0~6.0g/L, pH=7.0~8.0, calcium chloride solution
Mass fraction is 1%, adds distilled water until pH value reaches 6.0~7.0, time of repose is 10~15min.
Step（2）Described in cultivation temperature be 28~30 degrees Celsius, the mass fraction of calcium chloride solution is 1%, sealing training
It is 2~4 days to support the time, pH=5~6 of cushioning liquid, and regulation pH is 6.0~7.0.
Step（3）During middle addition ethanol, the volume ratio that ethanol mixes with zymotic fluid is 2:1, staticly settle the time for 30~
40min。
Step（4）Described in cushioning liquid pH=5~6, standard sieve size is 200 mesh.
Step（5）Described in heating after high temperature furnace temperature be 400~500 DEG C, roasting time is 2~3h.
Step（6）Described in microbial flocculant and palygorskite clay mixing quality ratio be 1:4.
The beneficial effects of the invention are as follows：
（1）Compared to common water purification catridge material, compound microbial water purification catridge material mainly includes two kinds of microorganism and clay
Flocculation activity material, inorganic water purification catridge material such as aluminium salt（Aluminum sulfate, aluminium polychloride etc.）, molysite（Iron chloride, polyaluminum sulfate
Iron etc.）If aluminium residual is excessive in water, not only to aquatile and plant pest, the aluminium osteopathy of the elderly can be also caused
And dementia, organic synthesis macromolecule water purification catridge material is due to economy and high efficiency, and practical application is most, however, it is same
When bring again environmental protection and health problem：First, its biological degradability is poor, second, its monomer or catabolite have toxicity, and
Compound microbial water purification catridge material has a variety of enzymes to absorb and purified because its composition is microorganism and clay in microbial body
Various salt ions in water, in the presence of microorganism not having salt ion spills into water, avoids secondary pollution, microorganism is main
Composition is protein, and after easily degradation is still decomposed after use, the spot of absorption is not easy and the organic group after decomposition
Depart from, still can adsorb firmly, some insoluble impurity of clay primary attachment are pollution-free, therefore the material for core drop of the present invention
Polluted after solution small；
（2）Effect of the inorganic ions in edaphon body is very important, and it is nutrition necessary to microorganism growth
Material, although some elements are very few in microorganism in-vivo content, it are hydrogen ion concentration, oxidation-reduction potential etc. and maintain pH value
The important component of balance, be that cell itself forms essential element, even, some microorganisms can by the use of inorganic salts as
The energy, it is hereby understood that inorganic salts have a great influence to the flocculation activity of the compound microbial producing strains, wherein magnesium sulfate, sulfuric acid
For the inorganic salts such as calcium for edaphon, demand is larger, and this is advantageous for having the material of flocculation activity to produce, micro- in addition
The metabolism of biology is very fast, can be unfavorable for its existence up to inorganic salt concentration is too high always present in water purification catridge material,
Microbial flocculation activity can just reduce, but this cycle is up to 1 year, thus made of obtained water purification catridge material of the invention
Filter core service life is grown.
Embodiment
It is in 7.0~8.0 aqueous suspension ofkaolins 1 that the pH that 3~5mL mass concentrations are 5.0~6.0g/L is added in test tube
~3mL mass fractions are 1% calcium chloride solution, mixing, add 40~45mL of distilled water to reach 6.0~7.0 up to pH value, are stood
10~15min, obtain substrate；Above-mentioned substrate is added in the triangular flask equipped with 2~3L sabouraud's agars, is 28 in temperature
At~30 DEG C, after sealing and standing culture 2~4 days, cultured products are obtained, by cultured products centrifuge with 2000~3000r/min
Rotating speed centrifugal treating 10~15min, isolated upper liquid, it is 1% to take above-mentioned upper liquid to be added to 250mL mass fractions
In calcium chloride solution, with the Na that pH is 5.82HPO3-NaH2PO3Cushioning liquid adjusts pH value to 6.0~7.0, is stirred with glass bar
3~5min, then 4~6min is stood, obtain pre-processing upper liquid；The above-mentioned pretreatment upper liquids of 50~60mL are taken out, by anhydrous second
Alcohol and the volume ratio 2 of pretreatment upper liquid:1 mixes both, staticly settles 30~40min, filtering, removes filtrate, is sunk
Form sediment；Respectively with the Na that pH is 5.82HPO3-NaH2PO3Cushioning liquid and distilled water wash precipitation 3~5 times successively, and by after washing
Precipitation naturally dry, and grind 30~40min after cross 200 mesh standard sieves, obtain sieve powder be microbial flocculant；Take
600~800g palygorskites are put into high temperature furnace, are heated to 400~500 DEG C, 2~3h of insulation roasting obtains palygorskite clay；
By microbial flocculant and palygorskite clay in mass ratio 1:4 mixing, are pressed into block filter core, you can obtain compound with compressor
Type microorganism water purification material for core.
Example 1
It is 7.0 aqueous suspension ofkaolins that the pH that 3mL mass concentrations are 5.0g/L is added in test tube, and 1mL mass fractions are 1% chlorine
Change calcium solution, mixing, add distilled water 40mL to reach 6.0 up to pH value, stand 10min, obtain substrate；Above-mentioned substrate is added and filled
In the triangular flask for there are 2L sabouraud's agars, in the case where temperature is 28 DEG C, after sealing and standing culture 2 days, cultured products is obtained, will be trained
Product centrifuge is supported with 2000r/min rotating speed centrifugal treating 10min, isolated upper liquid, takes above-mentioned upper liquid to add
In the calcium chloride solution for being 1% to 250mL mass fractions, with the Na that pH is 5.82HPO3-NaH2PO3Cushioning liquid adjust pH value to
6.0,3min is stirred with glass bar, then 4min is stood, obtain pre-processing upper liquid；The above-mentioned pretreatment upper liquids of 50mL are taken out, are pressed
Absolute ethyl alcohol and the volume ratio 2 of pretreatment upper liquid:1 mixes both, staticly settles 30min, filtering, removes filtrate, obtains
Precipitation；Respectively with the Na that pH is 5.82HPO3-NaH2PO3Cushioning liquid and distilled water wash precipitation 3 times successively, and by after washing
Precipitation naturally dry, and grind and 200 mesh standard sieves crossed after 30min, the powder that obtains sieving is microbial flocculant；Take 600g
Palygorskite is put into high temperature furnace, is heated to 400 DEG C, insulation roasting 2h obtains palygorskite clay；By microbial flocculant and
Palygorskite clay in mass ratio 1:4 mixing, are pressed into block filter core, you can obtain compound microbial water purification catridge with compressor
Material.
Example 2
It is 7.5 aqueous suspension ofkaolins that the pH that 4mL mass concentrations are 5.5g/L is added in test tube, and 2mL mass fractions are 1% chlorine
Change calcium solution, mixing, add distilled water 40mL to reach 6.5 up to pH value, stand 13min, obtain substrate；Above-mentioned substrate is added and filled
In the triangular flask for there are 2L sabouraud's agars, in the case where temperature is 29 DEG C, after sealing and standing culture 3 days, cultured products is obtained, will be trained
Product centrifuge is supported with 2500r/min rotating speed centrifugal treating 13min, isolated upper liquid, takes above-mentioned upper liquid to add
In the calcium chloride solution for being 1% to 250mL mass fractions, with the Na that pH is 5.82HPO3-NaH2PO3Cushioning liquid adjust pH value to
6.5,4min is stirred with glass bar, then 5min is stood, obtain pre-processing upper liquid；The above-mentioned pretreatment upper liquids of 55mL are taken out, are pressed
Absolute ethyl alcohol and the volume ratio 2 of pretreatment upper liquid:1 mixes both, staticly settles 35min, filtering, removes filtrate, obtains
Precipitation；Respectively with the Na that pH is 5.82HPO3-NaH2PO3Cushioning liquid and distilled water wash precipitation 4 times successively, and by after washing
Precipitation naturally dry, and grind and 200 mesh standard sieves crossed after 30min, the powder that obtains sieving is microbial flocculant；Take 700g
Palygorskite is put into high temperature furnace, is heated to 450 DEG C, insulation roasting 2.5h obtains palygorskite clay；By microbial flocculant
With palygorskite clay in mass ratio 1:4 mixing, are pressed into block filter core, you can obtain compound microbial water purification filtration with compressor
Core material.
Example 3
It is 8.0 aqueous suspension ofkaolins that the pH that 5mL mass concentrations are 6.0g/L is added in test tube, and 3mL mass fractions are 1% chlorine
Change calcium solution, mixing, add distilled water 45mL to reach 7.0 up to pH value, stand 15min, obtain substrate；Above-mentioned substrate is added and filled
In the triangular flask for there are 3L sabouraud's agars, in the case where temperature is 30 DEG C, after sealing and standing culture 4 days, cultured products is obtained, will be trained
Product centrifuge is supported with 3000r/min rotating speed centrifugal treating 15min, isolated upper liquid, takes above-mentioned upper liquid to add
In the calcium chloride solution for being 1% to 250mL mass fractions, with the Na that pH is 5.82HPO3-NaH2PO3Cushioning liquid adjust pH value to
7.0,5min is stirred with glass bar, then 6min is stood, obtain pre-processing upper liquid；The above-mentioned pretreatment upper liquids of 60mL are taken out, are pressed
Absolute ethyl alcohol and the volume ratio 2 of pretreatment upper liquid:1 mixes both, staticly settles 40min, filtering, removes filtrate, obtains
Precipitation；Respectively with the Na that pH is 5.82HPO3-NaH2PO3Cushioning liquid and distilled water wash precipitation 5 times successively, and by after washing
Precipitation naturally dry, and grind and 200 mesh standard sieves crossed after 40min, the powder that obtains sieving is microbial flocculant；Take 800g
Palygorskite is put into high temperature furnace, is heated to 500 DEG C, insulation roasting 3.0h obtains palygorskite clay；By microbial flocculant
With palygorskite clay in mass ratio 1:4 mixing, are pressed into block filter core, you can obtain compound microbial water purification filtration with compressor
Core material.
Comparative example
With the water purification catridge material of Hangzhou company production as a comparison case to compound microbial produced by the present invention
Water purification catridge material in water purification catridge material and comparative example is detected, and testing result is as shown in table 1：1. method of testing：
PH value test is detected using the online multiparameter water quality analyzer tables of 7000A.
How many milligrams of tests of soluble solid are detected using TDS water quality monitoring pens in 1 liter of water.
Colourity test is detected using chromascope.
Turbidity is detected using Laboratory Turbidimeter.
Chlorine residue is detected using the total chlorine analyzers of pocket chlorine residue.Table 1
It can be seen from data in table 1, soluble solid contains in compound microbial water purification catridge material produced by the present invention
Measure low, muddy thing is few, and the chlorine dose height remained in water has lasting sterilizing ability to water, has splendid water purification ability, tool
Have broad application prospects.
Claims (7)
1. a kind of preparation method of compound microbial water purification catridge material, it is characterised in that specifically preparation process is：
（1）3~5mL aqueous suspension ofkaolins are added in test tube, 1~3mL calcium chloride solutions, mixing, add 40~45mL of distilled water
Until pH value reaches certain value, stand, obtain substrate；
（2）Above-mentioned substrate is added in the triangular flask equipped with 2~3L sabouraud's agars, sealing and standing culture, production must be cultivated
Thing, by cultured products centrifuge with rotating speed centrifugal treating, isolated upper liquid, take above-mentioned upper liquid to be added to 250mL
Mass fraction is in 1% calcium chloride solution, uses Na2HPO3-NaH2PO3Cushioning liquid adjust pH value, with glass bar stirring 3~
5min, then 4~6min is stood, obtain pre-processing upper liquid；
（3）The above-mentioned pretreatment upper liquids of 50~60mL are taken out, absolute ethyl alcohol is mixed with pretreatment upper liquid, staticly settled, filters,
Filtrate is removed, is precipitated；
（4）Na is used respectively2HPO3-NaH2PO3Cushioning liquid and distilled water wash precipitation 3~5 times successively, and by the precipitation after washing
Naturally dry, and standard screen is crossed after grinding 30~40min, it is microbial flocculant to obtain sieving powder；
（5）Take 600~800g palygorskites to be put into high temperature furnace, be heated to certain temperature, insulation roasting obtains palygorskite and glued
Soil；
（6）Microbial flocculant and palygorskite clay are mixed, are pressed into block filter core with compressor, you can obtain compound micro-
Biological water purification material for core.
A kind of 2. preparation method of compound microbial water purification catridge material according to claim 1, it is characterised in that：Step
Suddenly（1）Described in aqueous suspension ofkaolin concentration be 5.0~6.0g/L, pH=7.0~8.0, calcium chloride solution mass fraction is
1%, add distilled water until pH value reaches 6.0~7.0, time of repose is 10~15min.
A kind of 3. preparation method of compound microbial water purification catridge material according to claim 1, it is characterised in that：Step
Suddenly（2）Described in cultivation temperature be 28~30 degrees Celsius, the mass fraction of calcium chloride solution is 1%, and sealing incubation time is 2
~4 days, pH=5~6 of cushioning liquid, regulation pH was 6.0~7.0.
A kind of 4. preparation method of compound microbial water purification catridge material according to claim 1, it is characterised in that：Step
Suddenly（3）During middle addition ethanol, the volume ratio that ethanol mixes with zymotic fluid is 2:1, it is 30~40min to staticly settle the time.
A kind of 5. preparation method of compound microbial water purification catridge material according to claim 1, it is characterised in that：Step
Suddenly（4）Described in Na2HPO3-NaH2PO3PH=5.8 of cushioning liquid, standard sieve size are 200 mesh.
A kind of 6. preparation method of compound microbial water purification catridge material according to claim 1, it is characterised in that：Step
Suddenly（5）Described in heating after high temperature furnace temperature be 400~500 DEG C, roasting time is 2~3h.
A kind of 7. preparation method of compound microbial water purification catridge material according to claim 1, it is characterised in that：Step
Suddenly（6）Described in microbial flocculant and palygorskite clay mixing quality ratio be 1:4.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1844360A (en) * | 2006-04-11 | 2006-10-11 | 湖南大学 | Microorganism flocculant producing strain by using bean dregs and process for producing same |
| CN101254969A (en) * | 2008-03-31 | 2008-09-03 | 清华大学 | Method for preparing microbial flocculant by using byproduct bacterial of fermentation industry |
| CN101327975A (en) * | 2008-07-31 | 2008-12-24 | 东北大学 | Method for preparing microorganism flocculant |
| CN101503709A (en) * | 2009-03-13 | 2009-08-12 | 厦门大学 | Method for preparing bioflocculation by Bacillus licheniformis |
| CN102107994A (en) * | 2010-12-23 | 2011-06-29 | 郑高宽 | Device for filtering various water sources into direct drinking water |
-
2017
- 2017-08-31 CN CN201710774328.2A patent/CN107381838A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1844360A (en) * | 2006-04-11 | 2006-10-11 | 湖南大学 | Microorganism flocculant producing strain by using bean dregs and process for producing same |
| CN101254969A (en) * | 2008-03-31 | 2008-09-03 | 清华大学 | Method for preparing microbial flocculant by using byproduct bacterial of fermentation industry |
| CN101327975A (en) * | 2008-07-31 | 2008-12-24 | 东北大学 | Method for preparing microorganism flocculant |
| CN101503709A (en) * | 2009-03-13 | 2009-08-12 | 厦门大学 | Method for preparing bioflocculation by Bacillus licheniformis |
| CN102107994A (en) * | 2010-12-23 | 2011-06-29 | 郑高宽 | Device for filtering various water sources into direct drinking water |
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