CN107376911A - 一种α‑钒酸银作为模拟酶的应用 - Google Patents

一种α‑钒酸银作为模拟酶的应用 Download PDF

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CN107376911A
CN107376911A CN201710623869.5A CN201710623869A CN107376911A CN 107376911 A CN107376911 A CN 107376911A CN 201710623869 A CN201710623869 A CN 201710623869A CN 107376911 A CN107376911 A CN 107376911A
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王毅
张盾
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Institute of Oceanology of CAS
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J23/00—Catalysts comprising metals or metal oxides or hydroxides, not provided for in group B01J21/00
    • B01J23/38—Catalysts comprising metals or metal oxides or hydroxides, not provided for in group B01J21/00 of noble metals
    • B01J23/54—Catalysts comprising metals or metal oxides or hydroxides, not provided for in group B01J21/00 of noble metals combined with metals, oxides or hydroxides provided for in groups B01J23/02 - B01J23/36
    • B01J23/66—Silver or gold
    • B01J23/68—Silver or gold with arsenic, antimony, bismuth, vanadium, niobium, tantalum, polonium, chromium, molybdenum, tungsten, manganese, technetium or rhenium
    • B01J23/682—Silver or gold with arsenic, antimony, bismuth, vanadium, niobium, tantalum, polonium, chromium, molybdenum, tungsten, manganese, technetium or rhenium with vanadium, niobium, tantalum or polonium

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Abstract

本发明涉及模拟酶技术，具体的说是一种α‑钒酸银作为模拟酶的应用。所述钒酸银作为模拟酶的应用。其是通过直接共沉淀法合成获得。本发明所述α‑钒酸银具有良好的HRP酶催化活性，在免疫分析等领域具有潜在应用前景，且廉价易得。

Description

一种α-钒酸银作为模拟酶的应用
技术领域
本发明涉及模拟酶技术，具体的说是一种α-钒酸银作为模拟酶的应用。
背景技术
过氧化物酶(Horseradish peroxidase，简称HRP)是由微生物或植物所产生的一类氧化还原酶，是以过氧化氢为电子受体催化底物氧化的酶。HRP是免疫酶标技术中最为常用的工具酶之一，常用于标记抗体。
文献(Nature Nanotechnology，2007，2，577-583)首次报道了Fe3O4磁性纳米颗粒具有HRP催化活性，其催化效率、机理和底物的专一性都与HRP相同。并且与HRP比较，还具有稳定性高、制备工艺简单和可循环利用的优点。在Fe3O4之后，又相继报道了氧化石墨烯、碳纳米管、氧化铈、氧化锰等纳米材料也具有HRP催化活性，在免疫分析和生物传感器领域得到了广泛引用。
α-钒酸银由于其独特的电子、光子和磁学特性，已经成为近年来的研究热点。α-钒酸银具有独特的晶体结构，其在生物领域的应用潜能尚未开发。
发明内容
本发明的目的是提供一种α-钒酸银作为模拟酶的应用。
为实现上述目的，本发明采用的技术方案为：
一种α-钒酸银作为模拟酶的应用。
所述α-钒酸银作为对底物进行催化氧化还原反应的HRP酶。
所述α-钒酸银通过直接共沉淀合成获得，为长度为2到10微米，宽度为500纳米左右棒状物。
所述α-钒酸银将AgNO3水溶液与NH4VO3的水溶液共混，于10-50℃条件下晶化0.5-2小时，晶化后洗涤，过滤，将滤饼在40-60℃干燥24小时，得到α-钒酸银。
具体为：
1)将NH4VO3配置成浓度为0.005-0.050mol/L的水溶液，再将AgNO3配置成浓度为0.005-0.050mol/L的水溶液，AgNO3溶液与NH4VO3溶液直接共混；
2)将上述混合溶液于10-50℃条件下晶化0.5-2小时，晶化后依次用去离子水、乙醇洗涤，过滤，将滤饼在40-60℃干燥24小时，得到化学组成为AgVO3的α-钒酸银模拟酶材料。
本发明所具有的有益效果：本发明所得模拟酶材料具有良好的HRP酶催化活性，在免疫分析等领域具有潜在应用前景，且廉价易得。
附图说明
图1为本发明实施例提供的模拟酶材料的XRD谱图(其中横坐标－角度2θ，单位为°(度)；纵坐标－强度，单位为a.u.(绝对单位))。
图2为本发明实施例提供的模拟酶材料的SEM图。
图3为本发明实施例提供的模拟酶材料的催化效果图(其中横坐标－时间Time，单位为s(秒)；纵坐标－吸光度值Abs)。
具体实施方式
实施例1
钒酸银制备过程：
将NH4VO3配置成浓度为0.029mol/L的水溶液，再将AgNO3配置成浓度为0.029mol/L的水溶液，AgNO3溶液与NH4VO3溶液直接共混，将上述混合溶液于30℃条件下晶化1小时，晶化后依次用去离子水、乙醇洗涤，过滤，将滤饼在60℃干燥24小时，得到化学组成为AgVO3的α-钒酸银(图1和2)。
由图1和2可见所合成产物为长度为2到10微米，宽度为500纳米左右微米级棒状α-钒酸银。
利用典型的催化氧化底物TMB和H2O2测定合成的钒酸银的类过氧化物酶活性。取一个1.5mL离心管，加入800μL磷酸盐缓冲液(100mM，pH 4.0)，然后分别加入1mM TMB和0.5mMH2O2作为催化底物，最后加入所合成的20μg/mL纳米模拟酶材料。当纳米模拟酶材料加入到反应体系后，能够用肉眼观察到体系迅速发生颜色变化(由无色变为蓝色)。所有的反应体系吸光度都通过U-2900Hitachi紫外分光光度计进行测定(时间扫描模式，波长652nm)。
实施例2-8
制备过程：
将NH4VO3配置成浓度为0.005～0.050mol/L的水溶液，再将AgNO3配置成浓度为0.005～0.050mol/L的水溶液，AgNO3溶液与NH4VO3溶液直接共混，将上述混合溶液于10-50℃条件下晶化0.5-2小时，晶化后洗涤，过滤，将滤饼在40-60℃干燥24小时，得到化学组成为AgVO3的α-钒酸银(参见表1)。
取一个1.5mL离心管，加入800μL磷酸盐缓冲液(100mM，pH 4.0)，然后分别加入1mMTMB和0.5mM H2O2作为催化底物，最后加入表1所合成的20μg/mL各纳米模拟酶材料。当纳米模拟酶材料加入到反应体系后，能够用肉眼观察到体系迅速发生颜色变化(由无色变为蓝色)。所有的反应体系吸光度都通过U-2900Hitachi紫外分光光度计进行测定(时间扫描模式，波长652nm)。表明所制备α-钒酸银有纳米酶活性(图3)。
表1

Claims (5)

1.一种α-钒酸银作为模拟酶的应用，其特征在于：所述α-钒酸银作为模拟酶的应用。
2.按权利要求1所述的α-钒酸银作为模拟酶的应用，其特征在于：所述α-钒酸银作为对底物进行催化氧化还原反应的HRP酶。
3.按权利要求1或2所述的α-钒酸银作为模拟酶的应用，其特征在于：所述α-钒酸银通过直接共沉淀合成获得长度为2—10微米，宽度为500纳米左右棒状物。
4.按权利要求3所述的α-钒酸银作为模拟酶的应用，其特征在于：所述α-钒酸银为将AgNO3水溶液与NH4VO3的水溶液共混，于10-50℃条件下晶化0.5-2小时，晶化后洗涤，过滤，将滤饼在40-60℃干燥24小时，得到α-钒酸银。
5.一种模拟酶，其特征在于：模拟酶为通过直接共沉淀合成获得长度为2—10微米，宽度为500纳米左右棒状物的α-钒酸银。
CN201710623869.5A 2017-07-27 2017-07-27 一种α‑钒酸银作为模拟酶的应用 Pending CN107376911A (zh)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113277558A (zh) * 2021-06-17 2021-08-20 湘潭大学 一种α′-AgVO3纳米材料的制备方法
CN115490264A (zh) * 2022-09-15 2022-12-20 山东黄海科技创新研究院 钒酸银模拟酶制备方法及其抑菌应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103506116A (zh) * 2012-06-29 2014-01-15 江南大学 钒酸银纳米管可见光光催化材料的制备及其应用
CN105217683A (zh) * 2015-10-14 2016-01-06 中国科学院海洋研究所 一种钒酸银作为模拟酶的应用

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
CN103506116A (zh) * 2012-06-29 2014-01-15 江南大学 钒酸银纳米管可见光光催化材料的制备及其应用
CN105217683A (zh) * 2015-10-14 2016-01-06 中国科学院海洋研究所 一种钒酸银作为模拟酶的应用

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113277558A (zh) * 2021-06-17 2021-08-20 湘潭大学 一种α′-AgVO3纳米材料的制备方法
CN115490264A (zh) * 2022-09-15 2022-12-20 山东黄海科技创新研究院 钒酸银模拟酶制备方法及其抑菌应用
CN115490264B (zh) * 2022-09-15 2023-10-20 山东黄海科技创新研究院 钒酸银模拟酶制备方法及其抑菌应用

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Application publication date: 20171124