CN107374979A - Purposes and captopril Dentin bonding of the angiotensin converting enzyme inhibitors such as captopril on Dentin adhensive - Google Patents
Purposes and captopril Dentin bonding of the angiotensin converting enzyme inhibitors such as captopril on Dentin adhensive Download PDFInfo
- Publication number
- CN107374979A CN107374979A CN201710540129.5A CN201710540129A CN107374979A CN 107374979 A CN107374979 A CN 107374979A CN 201710540129 A CN201710540129 A CN 201710540129A CN 107374979 A CN107374979 A CN 107374979A
- Authority
- CN
- China
- Prior art keywords
- dentin
- captopril
- adhensive
- bonding
- angiotensin converting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K6/00—Preparations for dentistry
- A61K6/80—Preparations for artificial teeth, for filling teeth or for capping teeth
Landscapes
- Health & Medical Sciences (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Plastic & Reconstructive Surgery (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses angiotensin converting enzyme inhibitors（Captopril etc.）In Dentin adhensive experiment and the purposes and method of clinic.Dentin matrix metalloproteinases（MMPs）The dentin collagen that can degrade fiber, it is unfavorable for the stabilization of Dentin adhensive.The angiotensin converting enzyme inhibitors such as described captopril, while have the function that to suppress MMPs, its sulfenyl（‑SH）With the Zn of enzyme2+With reference to middle=CO bases can be with the Hydrogenbond of enzyme, and a COOA base of its end can be combined with the arginine ionic bond of enzyme, reaches the purpose for suppressing clostridiopetidase A.Effect of the described captopril in Dentin adhensive is to suppress matrix metalloproteinase（MMPs）Degraded to dentin collagen fiber, so as to improve the stability of Dentin adhensive and persistence.With traditional MMPs inhibitor（Such as Chlorhexidine）Compare, captopril has more preferable physicochemical property, has important application value in the experiment and clinical research of Dentin adhensive.
Description
Technical field
The present invention relates to purposes of the angiotensin converting enzyme inhibitors such as captopril on Dentin adhensive and containing card
The Dentin bonding of Top's profit.
Background technology
Dentine is one and is made up of the hydroxyapatite rich in collagen stroma and the dentinal tubule containing working fluid
Bioplex.The Dentin adhensive in this heterogeneous structure and endogenous rich water matrix is established, depends on dentine
Resin glue monomer infiltration is entered in dentine, and caused micromechanics is sealed after solidification.However, resin monomer can not possibly be complete
The moisture of between full substitution collagenous fibres and collagen inner space, therefore, Dentin adhensive layer is likely to exist between collagenous fibres
Residual moisture, the close connection of resin and collagenous fibres is prevented, form Micro blazed-grating or nano leakage, cause the hydrolysis of resin component
Degraded, destroy Dentin adhensive.Moreover, the depth of depth and the resin penetration infiltration of dentine demineralization is possible to mismatch, meeting
Not exclusively infiltration area is produced in mixed layer bottom.External and experiment in vivo confirms, comes from this in the mixed layer of Dentin adhensive
The degraded of the collagen stroma in non-complete wetting area, the release and activation with endogenous dentine enzyme are relevant.Wherein, most important tooth
Essential endogenous enzyme is matrix metalloproteinase (MMPs).Exposed collagen is easily digested by endogenous MMPs, is had in dentine
The enzymolysis of machine matrix destroys the long-time stability that dentine-dummy can be threatened to bond.
MMPs is one group and depends on host's endogenous binding protein enzyme of zinc ion and calcium ion, and is present in the dentine of mineralising
In.MMPs point is clostridiopetidase A, gelatinase, extracellular matrix degrading enzyme, matrilysin, membranous type MMPs etc. and other.People dentine master
To include gelatin enzyme A (MMP-2), Gelatinase B (MMP-9) and clostridiopetidase A 2 (MMP-8), it is most main in these dentine that can degrade
The type i collagen fiber wanted.MMP-2 and MMP-9 can also degrade the extracellular matrix in basilar memebrane and other interstitial connective tissues.
MMPs present in dentine, unactivated state is in before sour demineralization.In dentine acid etching or the low ph value ring of carious dentin
Under border, they can be activated and discharge, and can cause the degraded of the collagenous fibres of exposure in dentine, so as to influence Dentin adhensive
Long-time stability.
Inactive proenzyme will be transformed into active MMPs, and classical method is turned off propetide area to realize.With MMP-9
Exemplified by, its activation mechanism is the coordinate bond between cysteine and zinc ion in crack protein molecule.When extraneous factor (cause a disease because
Element, acid etc.) coordinate bond between protein molecular internal cysteines and zinc ion has directly or indirectly been cut off, expose activity
Site, so as to activate MMP-9.So it can suppress MMP- with the Zinc Ions Chelated of MMP-9 avtive spots if any chemical substance
9 activity.Similar with MMP-9, all MMPs all rely on zinc ion, and its activation is required to and Zinc Ions Chelated.
Collagen egg in dentine mixed layer can be slowed down using NMPI (MMPs) in Dentin adhensive
White degraded, so as to improve the stability of Dentin adhensive.MMPs inhibitor has the former flower of a variety of, such as natural MMPs inhibitor
Pigment, grape seed extract, physiological MMPs inhibitor (tissue inhibitor of metalloproteinase,
TIMP) and synthesis MMPs inhibitor such as Chlorhexidine (chlorhexidine, CHX), ethylenediamine tetra-acetic acid (ethylene
Diaminetetraacetic acid, EDTA), glutaraldehyde, tetracycline etc. can increase the durability of dentine bonding.
Chlorhexidine (CHX) is to study the more non-specific MMP inhibitor for oral environment at present.Chlorhexidine is one
The antiseptic of kind wide spectrum, and cationic surface active agent, while be effective collagenase inhibitors, it can directly suppress MMP-
2, MMP-8, MMP-9 activity.Chlorhexidine is mainly caused to MMP-8 (clostridiopetidase A II) inhibitory action by cation chelation
's.Chlorhexidine can suppress MMP-8 activity in very low concentration (0.02%).Chlorhexidine is soluble in ethanol, but is slightly soluble in water
(0.08g/100ml).In order to improve solubility of the Chlorhexidine in water, it is water-soluble to be formed that acid is added generally in Chlorhexidine
Salt, such as chlorhexidine diacetate and chlorhexidine gluconate.Scholars the CHX of various concentrations is added in bonding agent or as
The component and silane coupling agent of dental resin are bonded for dentine, it is expected to increase the stability of Dentin adhensive.But CHX has secondary make
With, and effective combination of bonding agent and dentin matrix is may interfere with, remain and disputing on.
Captopril is artificial synthesized non-skin class angiotensin converting enzyme (ACE) inhibitor, and chemical composition is mercapto first
Third dried meat acid, chemical composition is 1- (3- sulfydryls -2- methyl isophthalic acids-propionyl)-L-PROLINE (C9H15NO3S).Structural formula is：
Mainly act on Re-A-A (RAA) system.The blood vessel that captopril suppresses RAA systems is tight
Plain converting Enzyme is opened, prevents angiotensinⅠ conversion or angiotensinⅡ, and Aldosterone Secretion can be suppressed, reduces water-sodium retention.
Captopril can be with the Zn in ACE avtive spots2+Chelating, so as to play the effect for suppressing ACE activity.Captopril is from 1979
Year plays the treatment applied to hypertension, has obvious antihypertensive effect to polytype hypertension, and can improve congestive heart failure
The cardiac function of patient is exhausted, while also serves as kidney protective agent and is using.
There are many research report captoprils inhibited to MMPs.From the molecular structure of captopril, its
One carboxyl of end can combine with the arginine ionic bond of enzyme, and its sulfydryl can be with the Zn of enzyme2+With reference to, and middle carbonyl energy
With the Hydrogenbond of enzyme, so as to reach suppress clostridiopetidase A purpose.
At present, sight is not concerned about Dentin adhensive field by the research that MMPs is acted on to captopril.Tooth sheet
It is a study hotspot that matter, which bonds Persistence study, is not fully solved.And MMPs is that influence Dentin adhensive is persistent
One of key factor.Early-stage Study is found, in Dentin adhensive, captopril can play it and suppress MMPs effect.And have
Mechanism body needs further to be studied.The Chlorhexidine of existing most study is a kind of broad spectrum antimicrobial agent, dissolving of the pure Chlorhexidine in water
Degree is very low, and the salt of Chlorhexidine has unstability in Dentin adhensive.On the contrary, good water solubility (the 0.1g/ of captopril
Ml), be also soluble in other solvents, such as ethanol, acetone, there is good physicochemical property, its mechanism of action also with Chlorhexidine not
It is identical to the greatest extent.Captopril has good MMPs rejection characteristics, and captopril etc. is introduced in Dentin adhensive, is brand-new taste
Examination.There is definite effect in the experiment of early stage, being expected to turn into improves the persistent new generation product of Dentin adhensive.
The content of the invention
Dentin collagen matrix (mainly collagenous fibres) can degrade in bonding, the stability of Dentin adhensive with persistently
Sex chromosome mosaicism is not fully solved also.The present invention introduces captopril etc. in the stability of Dentin adhensive, Persistence study, is
Brand-new trial, this is based on the angiotensin converting enzyme inhibitors such as captopril has suppression matrix metalloproteinase simultaneously
(MMPs) effect, and because its molecular weight is small, dissolubility is good, physicochemical property is outstanding, can be more convenient compared to Chlorhexidine etc.
Applied to Dentin adhensive.
The present invention solves the technical problem of in Dentin adhensive experiment in vitro apply captopril and with tradition
MMPs inhibitor Chlorhexidines compare research, to determine whether be advantageous to remain viscous using captopril in Dentin adhensive
The stability and persistence of knot.The Dentin bonding containing captopril etc. of correlation is developed on this basis, and verifies it
Validity, finally in clinical application.
In order to reach this purpose, the technical scheme is that, angiotensin converting enzyme inhibitors are applied in tooth sheet
On matter bonds.
In order to reach this purpose, according to an embodiment, turn it is a feature of the present invention that choosing a kind of angiotensins
Change enzyme inhibitor-captopril, its chemical composition is captopril, and chemical composition is 1- (3- sulfydryl -2- methyl isophthalic acids-the third
Acyl)-L-PROLINE (C9H15NO3S).
Captopril is only one kind of angiotensin converting enzyme inhibitors, its in angiotensin converting enzyme inhibitors
His form preparation, such as enalapril, Cilazapril, quinapril, Benazepril, fosinopril, Ramipril, it may have class
As effect and effect, the angiotensin converting enzyme inhibitors that preferred molecular weight is small, dissolubility is good.Theirs can also be used
The mixture of arbitrary proportion, any component.
Molten reagent can be made in angiotensin converting enzyme inhibitors including captopril, and its solvent is water, second
Alcohol, acetone etc.；Binding agent can also be made, the angiotensin converting enzyme inhibitors including captopril are as binding agent
One of which component.
The technical solution adopted by the present invention be Dentin adhensive experiment in vitro research in, captopril with research compared with into
Ripe MMPs inhibitor-Chlorhexidine compares.Complete bond after, the present invention by measure Dentin adhensive intensity change,
The research methods such as SEM, TEM evaluation captopril is applied to the effect in Dentin adhensive.
In the contrast experiment of captopril and Chlorhexidine studies：The concentration of chlorhexidine gluconate is 2%；Captopril
Water is dissolved in, is prepared into the solution that concentration is 0.5%, 1%, 2%；Control group is then distilled water.
Isolated Tooth prepares the dentin bonding of standard, respectively packet, 2% Chlorhexidine group, Captopril group
(0.5%, 1%, 2%) and control group (deionized water).Dentin bonding is after acid etching under the same terms, above-mentioned solution
The dentin bonding each organized is handled respectively, completes to bond.Then micro-stretching test specimen is prepared, enters the aging examination of water-filling storage
Test.(24 hours), 6 months and the adhesion strength after 12 months at once, the change ESEM of adhesive surface are determined respectively
(SEM) observe.
According to adhesion strength and the Comparative result captopril of ESEM and the effect of Chlorhexidine, captopril is inferred
Application effect in Dentin adhensive.
On this experiment basis, make further research.Because the solvent of Dentin bonding is usually ethanol, acetone
Deng, the technical solution adopted by the present invention is in the experiment in vitro research of Dentin adhensive, and captopril is dissolved in ethanol, acetone,
It is prepared into certain density captopril ethanol solution, captopril acetone soln.Dentin bonding is handled with the solution, it is complete
Into bonding.Then degradation is carried out.Adhesion strength is finally determined, with ESEM, transmission electron microscope etc. for support study dies means.
Captopril is evaluated using ethanol, acetone as solvent, the application effect in Dentin adhensive.
The further technical scheme that the present invention uses is that captopril is directly added into Dentin adhensive as a kind of component
In agent, the Dentin bonding containing captopril is prepared.In vitro in experimental study, the viscous of dentine is completed using this binding agent
Knot.Then degradation is carried out.Adhesion strength is finally determined, with ESEM, transmission electron microscope etc. for support study dies means.Evaluation
Dentin bonding containing captopril, the application effect in Dentin adhensive.
By above-mentioned experiment in vitro, it is determined that it is molten to prepare the certain density aqueous solution containing captopril, ethanol solution, acetone
The preparations such as liquid；It is determined that prepare the Dentin bonding containing a certain amount of captopril.By clinical test, captopril system is evaluated
Agent, the clinical application effect of captopril binding agent.
By purposes provided by the invention, method, binding agent, the collagenous fibres in dentin matrix can be protected not divided
Solution is destroyed, and improves the intensity of Dentin adhensive, maintains the stability and persistence of Dentin adhensive.
Brief description of the drawings
Table 1 is that (deionized water, 2% chlorhexidine gluconate, 0.5% Kato are general for different dentin conditioners of the invention
Profit, 1% captopril, 2% captopril) (24 hours), 6 months, the Dentin adhensive intensity level of 12 months at once after processing.
Fig. 1 is that (deionized water, 2% chlorhexidine gluconate, 0.5% Kato are general for different dentin conditioners of the invention
Profit, 1% captopril, 2% captopril) (24 hours), 6 months, the Dentin adhensive intensity of 12 months at once after processing
Block diagram.
Fig. 2 is that (deionized water, 2% chlorhexidine gluconate, 0.5% Kato are general for different dentin conditioners of the invention
Profit, 1% captopril, 2% captopril) processing after Dentin adhensive test test specimen pass through micro-tension test, Dentin adhensive
The site analysis figure of plane of disruption fracture.
Fig. 3 is that (deionized water, 2% chlorhexidine gluconate, 0.5% Kato are general for different dentin conditioners of the invention
Profit, 1% captopril, 2% captopril) after processing, (24 hours), 6 months, the test of 12 months Dentin adhensives at once
The scanning electron microscope (SEM) photograph of test specimen plane of disruption after micro-tension test.
Embodiment
Presently preferred embodiments of the present invention is described in detail below in conjunction with the accompanying drawings, so that advantages and features of the invention energy
It is easier to be readily appreciated by one skilled in the art, apparent is clearly defined so as to be made to protection scope of the present invention.
Accompanying drawing is referred to, the embodiment of the present invention includes：
Embodiment 1
Tested based on the present embodiment, compare captopril and traditional MMPs inhibitor Chlorhexidines in Dentin adhensive
Action effect.Specific method is as follows：
Captopril (Sigma) is prepared into 0.5%, 1%, 2% aqueous solution, the gluconic acid chlorine that Chlorhexidine is 2% oneself
Fixed (Alfa Aesar, USA) aqueous solution.
Without dental caries third molar 40,5 groups, every group 8 are randomly divided into.First group：Handled with deionized water, as control
Group；Second group：Handled with 2% Chlorhexidine；3rd group：The processing of 0.5% captopril；4th group：The processing of 1% captopril；The
Five groups：The processing of 2% captopril.
Every tooth cuts hat side's enamel, exposure dentine, 800 mesh carbon of surface in spray underwater vertical in tooth long axile
The wet tumbling of SiClx sand paper prepares dentin bonding, and surrounding axial wall enamel is abraded parallel to tooth long axile.
Dentin bonding first uses etching agent acid etching 20 seconds, then a large amount of hydroblastings 30 seconds, air blow drying (10 seconds)
Afterwards.Every group of dentin bonding carries out rewetting with different solutions respectively and handled 60 seconds：First group of painting deionized water, second group of coating
2% chlorhexidine, the 3rd group of captopril solution of coating 0.5%, the 4th group of captopril solution of coating 1%, the 5th group of painting
The captopril solution of cloth 2%.
Redundant solution is sucked with moisture absorption paper, becomes micro- moisture state, then even spread binding agent (3M, AdperTM
Single bond 2, the U.S.) 20 seconds, air featheriness 10 seconds, illumination carries out resin (3M, Z250) slicing and filling, tree after 20 seconds
Fat packed height is about 4-5mm.
The tooth that resin fill finishes is being cut at a slow speed on dental disk machine along long axis of tooth, is formed cross-sectional area and is about
0.9mm*0.9mm dentine test test specimen, length is about 8mm~12mm, and 16 test test specimens are about prepared per tooth.
The dentine test test specimen of every tooth is separately stored in the airtight bottle of deionized water.Deionized water is changed weekly
Once.
Randomly select 4 samples in sample after 24 hours prepared by every group of every tooth, every group of totally 32 samples, i.e.,
Quarter group microtensile bond strength measure.
Remaining dentine test test specimen, which is stored in the deionized water in airtight bottle, carries out degradation, and deionized water is weekly
Change once.Same amount of (32) progress micro-stretchings of dentine test test specimen are taken after 6 months and at random after 12 months respectively
Experiment (method with 24 hours at once group).
The instrument of microtensile bond strength measure：Universal testing machine (Zwick/Roell Model BZ2.5/TN1S,
Zwick GmbH＆Co, Ulm, Germany) 0.5N, tension test speed 1mm/min are preloaded, until dentine test specimen adhesive surface is broken,
Computer records the peak value (N) during sample tension failure automatically.Record the width and thickness for being measured test specimen respectively simultaneously.According to
Area (the mm of peak value (N) and adhesive surface during fracture2), the micro-tensile bond strength value (MPa=N/ of calculating each test specimen of each group
mm2)。
Each group microtensile bond strength test number uses the statistical analysis of 16.0 statistical softwares of SPSS, between each group mean
Total difference compared using one-way analysis of variance (One-way ANOVA), compare two-by-two between mean and carried out with LSD methods.
The SEM observations of the dentine test test specimen plane of disruption：After sample carries out micro-tension test in each period, platinum is sprayed
After processing, it is fine that SEM (90,500,1.5K, 3K, 10K, 20K) under different amplification observes plane of disruption dentinal tubule, collagen
Tie up the structures such as net, resin be prominent.
Each experimental group adhesion strength value is shown in Table 1：
Statistical result showed：At once the average microtensile bond strength value between group (24 hours) each group does not have conspicuousness poor
Different (p>0.05).With the passage of time, the microtensile bond strength value of each group has declined, control group and 2% Chlorhexidine group
Decline substantially, and captopril each group adhesion strength declines degree and does not have control group and 2% Chlorhexidine group obvious.6 months groups：
The micro-stretching average strength of 2% Chlorhexidine group is higher than control group, but does not have significant difference between the two；1% captopril
Group micro-stretching average strength is higher than control group, and has significant difference (p<0.05)；2% Captopril group micro-stretching intensity is put down
Average is respectively higher than control group and 2% Chlorhexidine group, and has significant difference (p<0.05).12 months groups：The micro- drawing of control group
Stretching average strength and 2% Chlorhexidine group does not relatively have significant difference (p>0.05)；0.5%th, 1% and 2% Captopril group
Micro-stretching average strength and control group, 1% Chlorhexidine group have significant difference (p<0.05)；0.5%th, 1% and
There is no significant difference (p between the micro-stretching average strength group of 2% Captopril group>0.05).
Control group (24 hours), 6 months, the comparison of the average microtensile bond strength value of 12 months at once：With the time
The average microtensile bond strength of passage declines, and 6 months are 29.89 ± 14.55MPa, it is reduced within 12 months 23.90 ±
14.56MPa, more there is significant difference (p with the microtensile bond strength that is averaged at once<0.05), 6 months and 12 months
Adhesion strength relatively there is no significant difference (p>0.05).2% Chlorhexidine group 6 months, the average micro-stretching of 12 months bond
Intensity level equally has decline, wherein average microtensile bond strength value after 12 months decline it is apparent (be reduced to 20.12 ±
13.61MPa), 6 months when average microtensile bond strength value and organize at once and relatively there is no significant difference (p>0.05), and
Average microtensile bond strength value at 12 months has a significant difference compared with group at once, and with average micro- drawing at 6 months
Stretching adhesion strength value more also has significant difference (p<0.05).0.5% Captopril group, 1% Captopril group, 2% Kato
Though the average microtensile bond strength value of Puli's group has declined with the passage of time, decline unobvious (table 1), 24
Triangular average microtensile bond strength value does not have significant difference more in hour, 6 months, 12 months three periods
(p>0.05)。
The average microtensile bond strength result of three periods shows that compared with Chlorhexidine, captopril is more favourable
In the Dentin adhensive intensity for maintaining stabilization.Fig. 2 is the block diagram of different groups of adhesion strength changes, intuitively understands this experiment
Conclusion.
Micro-stretching test test specimen plane of disruption analysis result is shown in accompanying drawing 2.At once when (24 hours), what 5 groups of adhesive surfaces were broken
Position is essentially identical, based on resin, tack coat；After 6 months, the fracture location slight change of 5 groups of adhesive surfaces, each other relatively
Change unobvious；After 12 months, the control group of deionized water processing and the position of 2% Chlorhexidine group fracture location change substantially,
Main plane of disruption position is related to dentine, collagen fiber layer in the basalis of adhesive surface, and the reality of 3 groups of captopril processing
A group adhesive surface fracture location change unobvious are tested, it is similar to organizing at once.As a result prompting captopril is more beneficial for Dentin adhensive,
It is able to maintain that its stability and persistence.
SEM results, are shown in Fig. 3.
At once group (24 hours)：Each group plane of disruption pattern is similar, and resin is prominent to be tightly combined with dentinal tubule, tooth sheet between pipe
There is more resin component in matter, wrap collagenous fiber network, collagen meshwork is unintelligible.
6 months groups：Resin is dashed forward in control group dentinal tubule there is obvious gap (A2 white arrows) between small tube wall, manages
Between in dentine resin component have signs of degradation, the more exposed collagen (A2 black arrows) of exposure.Resin in 2% Chlorhexidine group
It is prominent to be tightly combined most of with dentinal tubule, occur crack a little, visible more resin component, collagen fibre in dentine between pipe
Dimension network structure exposes on a small quantity.In three groups of captopril, appearance structure is similar, and resin is prominent most of to be still filled with whole tooth sheet
Matter tubule and it is tightly combined, still with a large amount of resin components in dentine between pipe, collagenous fiber network is because of binding resin composition, collagen
Network structure is still unintelligible.
12 months groups：Compared with group at once, the control group after 12 months changes greatly with 2% Chlorhexidine group pattern, tree
The prominent gap between dentinal tubule of fat becomes larger (A3, B3 white arrow), and resin is dashed forward degraded in itself, in the tubule of part
The prominent missing of resin.Because resin component degraded is more in dentine between pipe, exposed collagenous fibres are common (A3, B3 black arrow),
Collagen meshwork is clear.In three groups of captopril, pattern is similar, still has tree with organizing at once in more most of dentinal tubules
Fat is dashed forward, and wherein most of resins are dashed forward and are tightly combined with dentinal tubule wall, and crack occurs in minority；Still protected in dentine between pipe
More resin component is stayed, thus most of collagen meshwork is still unintelligible.
SEM experimental results show that captopril can maintain the stability and persistence of Dentin adhensive, in this experiment its
Effect is better than Chlorhexidine.
Microtensile bond strength shows that captopril can more maintain stable Dentin adhensive with SEM results, in dentine
Bond repair in there is application value.
Embodiment 2
The solvent of Dentin bonding generally has ethanol, acetone etc., and captopril is soluble in ethanol, acetone, with ethanol, third
Ketone is that solvent prepares certain density captopril ethanol solution, captopril acetone soln.It is then applied to Dentin adhensive,
Pass through its influence to Dentin adhensive intensity of experiment test.For the method and step of experiment with reference to embodiment 1, the method for implementation is micro-
The measure of tensile bond strength, ESEM, transmission electron microscope etc..
Embodiment 3
The further technical scheme that the present invention uses is that captopril is directly added into Dentin bonding, and preparation contains
The Dentin bonding of captopril.The experiment in vitro for making Dentin adhensive with Isolated Tooth is studied.Equally prepare Dentin adhensive
Face, dentin bonding are coated with the binding agent containing captopril, illumination after acid etching, flushing, then complete resin layering and fill
Fill out.And the Dentin bonding with the Dentin bonding without captopril, containing other compositions (such as Chlorhexidine) is compared and ground
Study carefully.The method of test has the SEM for determining, bonding plane of disruption observations and transmission electron microscope of microtensile bond strength etc..Evaluation contains
The Dentin bonding of captopril, the application effect in Dentin adhensive.
Embodiment 4
The final purpose of the present invention is clinical practice.By above-mentioned experiment in vitro, it is determined that preparing certain density containing card
The preparations such as the sharp aqueous solution of Top, ethanol solution, acetone soln；It is determined that prepare the Dentin adhensive containing a certain amount of captopril
Agent.Pass through clinical test, evaluation captopril preparation, the clinical application effect of captopril binding agent.The method choice of implementation
Suitable case (such as V-type hole), complete to bond filling, in accordance with certain standard, by long term follow-up, with retention rate or come off
The methods of rate, edge analysis, comparative study captopril preparation, the clinical effectiveness of captopril binding agent application.
Embodiment 1 is the infrastest of the present invention, and this is test result indicates that captopril is maintaining the steady of Dentin adhensive
It is better than Chlorhexidine in qualitative and persistence, illustrates that captopril has application value in Dentin adhensive.Remaining embodiment
It is the amplification on this experiment basis, while there may also be other embodiments.So blood vessel such as any relevant captopril
The directly or indirectly application in the related technical field of Dentin adhensive of Angiotensin Converting enzyme inhibitor, is included in the present invention
Scope of patent protection within.
Claims (10)
1. purposes of the angiotensin converting enzyme inhibitors on Dentin adhensive.
2. purposes of the angiotensin converting enzyme inhibitors in Dentin bonding.
3. purposes of the captopril on Dentin adhensive.
4. purposes of the captopril in Dentin bonding.
5. Dentin bonding, it is characterised in that it contains angiotensin converting enzyme inhibitors.
6. Dentin bonding, it is characterised in that it contains captopril.
7. improve the method for Dentin adhensive, it is characterised in that angiotensin converting enzyme inhibitors are directly dissolved in water and are made by it
Inhibitor solution, prepared inhibitor solution are directly coated at the dentin surface after acid etching, angiotensin converting enzyme suppression
Preparation profit is fully infiltrated through in dentin matrix, is coated Dentin bonding and is completed Dentin adhensive.
8. improve the method for Dentin adhensive, it is characterised in that captopril is directly dissolved in water and inhibitor solution is made by it, institute
The inhibitor solution of preparation is directly coated at the dentin surface after acid etching, and captopril profit fully infiltrates through dentin matrix
In, it is coated Dentin bonding and completes Dentin adhensive.
9. the method for the raising Dentin adhensive such as claim 7 or 8, it is characterised in that the solution is water, ethanol or acetone
Solution.
10. improve the method for Dentin adhensive, it is characterised in that it uses the Dentin bonding of claim 5 or 6, dentine
After acid etching, dentin surface of the adhesive-coated after acid etching completes to bond.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710540129.5A CN107374979A (en) | 2017-07-05 | 2017-07-05 | Purposes and captopril Dentin bonding of the angiotensin converting enzyme inhibitors such as captopril on Dentin adhensive |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710540129.5A CN107374979A (en) | 2017-07-05 | 2017-07-05 | Purposes and captopril Dentin bonding of the angiotensin converting enzyme inhibitors such as captopril on Dentin adhensive |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107374979A true CN107374979A (en) | 2017-11-24 |
Family
ID=60335043
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710540129.5A Pending CN107374979A (en) | 2017-07-05 | 2017-07-05 | Purposes and captopril Dentin bonding of the angiotensin converting enzyme inhibitors such as captopril on Dentin adhensive |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107374979A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104398390A (en) * | 2014-11-24 | 2015-03-11 | 浙江大学 | Dentin primary coating-washing primary coating agent and method thereof |
| CN105342863A (en) * | 2015-12-11 | 2016-02-24 | 张凌 | Application of epigallocatechingallate (EGCG) in improving dental resin adhesive material restoration performance |
-
2017
- 2017-07-05 CN CN201710540129.5A patent/CN107374979A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104398390A (en) * | 2014-11-24 | 2015-03-11 | 浙江大学 | Dentin primary coating-washing primary coating agent and method thereof |
| CN105342863A (en) * | 2015-12-11 | 2016-02-24 | 张凌 | Application of epigallocatechingallate (EGCG) in improving dental resin adhesive material restoration performance |
Non-Patent Citations (2)
| Title |
|---|
| C. SABATINI: "《Effect of a Chlorhexidine-containing Adhesive on Dentin Bond Strength Stability》", 《OPERATIVE DENTISTRY》 * |
| 朱赟婕: "《卡托普利和氯己定对牙本质粘结持久性影响的对比研究》", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Mobarak | Effect of chlorhexidine pretreatment on bond strength durability of caries–affected dentin over 2-year aging in artificial saliva and under simulated intrapulpal pressure | |
| Perdigão | Current perspectives on dental adhesion:(1) Dentin adhesion–not there yet | |
| Frassetto et al. | Mechanisms of degradation of the hybrid layer in adhesive dentistry and therapeutic agents to improve bond durability—A literature review | |
| Perdigao et al. | Fundamental concepts of enamel and dentin adhesion | |
| Sánchez-Ayala et al. | Marginal microleakage of class V resin-based composite restorations bonded with six one-step self-etch systems | |
| Li et al. | The role of functional monomers in bonding to enamel: Acid–base resistant zone and bonding performance | |
| Feitosa et al. | Degradation of adhesive-dentin interfaces created using different bonding strategies after five-year simulated pulpal pressure | |
| Sangwichit et al. | Effect of thermocycling on the durability of etch-and-rinse and self-etch adhesives on dentin | |
| Ahuja et al. | Enamel deproteinization before acid etching–A scanning electron microscopic observation | |
| Comba et al. | Effect of benzalkonium chloride on dentin bond strength and endogenous enzymatic activity | |
| Belli et al. | Slow progression of dentin bond degradation during one-year water storage under simulated pulpal pressure | |
| Ajami et al. | Effect of three different contamination removal methods on bond strength of a self-etching adhesive to dentin contaminated with an aluminum chloride hemostatic agent | |
| Khoroushi et al. | Effect of thermocycling and water storage on bond longevity of two self-etch adhesives | |
| Yu et al. | Epigallocatechin-3-gallate/nanohydroxyapatite platform delivery approach to adhesive-dentin interface stability | |
| Kumar et al. | Bond failure and its prevention in composite restoration-a review | |
| Sabatini et al. | Effect of desensitizing agents on the bond strength of mild and strong self-etching adhesives | |
| Kazak et al. | Development of dentin bonding systems from past to present | |
| Singh et al. | Effect of EDTA conditioning and carbodiimide pretreatment on the bonding performance of all‐in‐one self‐etch adhesives | |
| Hubbezoğlu et al. | The efficacy of four cavity disinfectant solutions and two different types of laser on the micro-shear bond strength of dentin adhesives | |
| Daood et al. | New antimicrobial and collagen crosslinking formulated dentin adhesive with improved bond durability | |
| Hamama et al. | Effect of chemomechanical caries removal on bonding of resin‐modified glass ionomer cement adhesives to caries‐affected dentine | |
| Shirban et al. | A new solvent-free one-step self-etch adhesive: bond strength to tooth structures | |
| Mine et al. | Bonding effectiveness and interfacial characterization of a HEMA/TEGDMA-free three-step etch&rinse adhesive | |
| Zawaideh et al. | Bonding of Resin Composite to Caries-affected Dentin after Carisolv® Treatment | |
| Bravo Palma et al. | Effect of 2% chlorhexidine on dentin shear bond strength of different adhesive systems: A 6 months evaluation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20171124 |
|
| WD01 | Invention patent application deemed withdrawn after publication |