CN107373677A - A kind of extracting method of pig bone extract solution - Google Patents
A kind of extracting method of pig bone extract solution Download PDFInfo
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- CN107373677A CN107373677A CN201710709940.1A CN201710709940A CN107373677A CN 107373677 A CN107373677 A CN 107373677A CN 201710709940 A CN201710709940 A CN 201710709940A CN 107373677 A CN107373677 A CN 107373677A
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- extract solution
- bone
- extraction
- pig
- pig bone
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
Abstract
The invention belongs to polypeptide to extract field, proposes a kind of extracting method of pig bone extract solution, comprises the following steps：Step 1：Pretreatment, pig bone is ground into after pretreatment broken bone；Step 2：First extraction, broken bone is inserted in Rotating Stainless Steel Cage double-edged fine-toothed comb to the purified water for adding 1.5 2.5 times of broken bone weights, 130 ± 2 DEG C of hot pressing extraction 3h of boiling 2 of keeping temperature, obtains first extract solution, first extract solution is sent into cooling tank；Step 3：Second extraction, the broken bone after first extraction is inserted in Rotating Stainless Steel Cage double-edged fine-toothed comb to the purified water for adding equivalent weight, 130 ± 2 DEG C of hot pressing extraction 2h of boiling 1 of keeping temperature, obtains secondary raffinate；Step 4：First extract solution and secondary raffinate are merged, post-processed to obtain the pig bone extract solution for the peptide molecule chain for being less than 4000 dalton containing molecular weight.This method can effectively remove hmw protein present in conventional method.
Description
Technical field
The present invention relates to polypeptide to extract field, particularly a kind of extracting method of pig bone extract solution.
Background technology
The pig bone extract solution production extracting method used before our factory is that net pig limbs bone is heated and boiled, rejecting manadesma and residual
Meat, then crush skeletonization.Put into and add twice of purified water in extractor, after being heated to 130 DEG C, 130 ± 2 DEG C of hot pressing of keeping temperature carry
2h is taken, extract solution is sent into cooling tank.The purified water for adding broken bone equivalent for the second time is same as above method and extracted again once, and merging is extracted twice
Liquid is to cooling tank.
The defects of this method, is, is decocted using high temperature and high pressure method, first time 2.5h, second of 1.5h, pig limbs bone
Broken bone decocts into thick soup, and the later stage will pass through condensation, centrifugation, ultrafiltration, freezing, again thawing, the refinement treatment process such as ultrafiltration, it is difficult to high
The impurity treatments such as molecule protein thoroughly remove.
It is existing and the pig bone extract solution required for bone melon parenteral solution is to need the peptide molecule chain less than 4000 dalton
Extractive technique can not entirely reach needs, pig bone extract solution efficient liquid phase differentiates that item is constantly present impurity peaks, and yield is low, miscellaneous
Matter separation is difficult, it is impossible to has absorption maximum at constant peak.
The content of the invention
A kind of the present invention is intended to provide extracting method of the high pig bone extract solution of macromolecule protein clearance.
Its concrete scheme is：A kind of extracting method of pig bone extract solution, comprises the following steps：
Step 1：Pretreatment, pig bone is ground into after pretreatment broken bone；
Step 2：First extraction, broken bone is inserted in Rotating Stainless Steel Cage double-edged fine-toothed comb to the purified water for adding 1.5-2.5 times of broken bone weight,
130 ± 2 DEG C of hot pressing of keeping temperature extraction boiling 2-3h, Stress control obtain first extract solution, just in 0.2MPa ± 0.05MPa
Secondary extract solution is sent into cooling tank；
Step 3：Second extraction, the broken bone after first extraction is inserted the pure of equivalent weight is added in Rotating Stainless Steel Cage double-edged fine-toothed comb
Change water, 130 ± 2 DEG C of hot pressing of keeping temperature extraction boiling 1-2h, Stress control obtains second extraction in 0.2MPa ± 0.05MPa
Liquid；
Step 4：First extract solution and secondary raffinate are merged, post-processed to obtain containing molecular weight less than 4000
The pig bone extract solution for the peptide molecule chain that you pause.
In the extracting method of above-mentioned pig bone extract solution, described pig bone is the Limb bone of pig.
In the extracting method of above-mentioned pig bone extract solution, the method for described pretreatment is specially：Clean pig limbs bone
And heat and boil, manadesma and residual meat are rejected, then crush.
In the extracting method of above-mentioned pig bone extract solution, the method for the post processing in described step 4 is：After merging
Extract solution by condensation, centrifugation, ultrafiltration, freezing, thawing, again ultrafiltration removal macromolecule protein impurity, obtain containing molecular weight
Less than the pig bone extract solution of the peptide molecule chain of 4000 dalton.
The beneficial effects of the present invention are：
Compared with existing pig bone extract solution extracting method (HTHP decocting method), this programme, which is easy to extract pig bone, to be carried
Take the peptide molecule chain less than 4000 dalton required for liquid, the pig bone of the HTHP boiling extracting method extraction of this programme
Extract solution, efficient liquid phase differentiate that item is all qualified, there is absorption maximum at constant peak.
Embodiment
With reference to embodiment, technical scheme is described in further detail, but not formed pair
Any restrictions of the present invention.
In order to which more clearly the present invention will be described, embodiment is listed below to illustrate the superiority of the present invention.
Embodiment 1
A kind of new extracting method of pig bone extract solution, specific method are as follows：First middle part plus a stainless steel in extractor
Cage double-edged fine-toothed comb is standby, and pretreatment process is equally that net pig limbs bone heating is boiled, and rejects manadesma and residual meat, then crush skeletonization.Input
Add twice of purified water in extraction cage double-edged fine-toothed comb, after being heated to 130 DEG C, 130 ± 2 DEG C of hot pressing extraction boiling 2.5h of keeping temperature,
Stress control is sent into cooling tank in 0.2MPa ± 0.05MPa, extract solution.The purified water for adding broken bone equivalent for the second time is same as above method again
Extract 1.5h, Stress control in 0.2MPa ± 0.05MPa, merge twice extract solution to cooling tank.Extract solution after merging is passed through
Condensation, centrifugation, ultrafiltration, freezing, again thawing, ultrafiltration removal macromolecule protein impurity are crossed, obtains being less than 4000 containing molecular weight
The pig bone extract solution for the peptide molecule chain that you pause.
Embodiment 2
A kind of new extracting method of pig bone extract solution, specific method are as follows：First middle part plus a stainless steel in extractor
Cage double-edged fine-toothed comb is standby, and pretreatment process is equally that net pig limbs bone heating is boiled, and rejects manadesma and residual meat, then crush skeletonization.Input
Add twice of purified water in extraction cage double-edged fine-toothed comb, after being heated to 130 DEG C, 130 ± 2 DEG C of hot pressing extraction boiling 2h of keeping temperature, pressure
Power control is sent into cooling tank in 0.2MPa ± 0.05MPa, extract solution.The purified water for adding broken bone equivalent for the second time is same as above method and carried again
Take 2h, Stress control in 0.2MPa ± 0.05MPa, merge twice extract solution to cooling tank.By the extract solution after merging through supercooling
Solidifying, centrifugation, ultrafiltration, freezing, again thawing, ultrafiltration removal macromolecule protein impurity, obtain being less than 4000 dalton containing molecular weight
Peptide molecule chain pig bone extract solution.
Embodiment 3
A kind of new extracting method of pig bone extract solution, specific method are as follows：First middle part plus a stainless steel in extractor
Cage double-edged fine-toothed comb is standby, and pretreatment process is equally that net pig limbs bone heating is boiled, and rejects manadesma and residual meat, then crush skeletonization.Input
Add twice of purified water in extraction cage double-edged fine-toothed comb, after being heated to 130 DEG C, 130 ± 2 DEG C of hot pressing extraction boiling 3h of keeping temperature, pressure
Power control is sent into cooling tank in 0.2MPa ± 0.05MPa, extract solution.The purified water for adding broken bone equivalent for the second time is same as above method and carried again
Take 1h, Stress control in 0.2MPa ± 0.05MPa, merge twice extract solution to cooling tank.By the extract solution after merging through supercooling
Solidifying, centrifugation, ultrafiltration, freezing, again thawing, ultrafiltration removal macromolecule protein impurity, obtain being less than 4000 dalton containing molecular weight
Peptide molecule chain pig bone extract solution.
The present embodiment 1-3 new pig bone extract solution production extracting method, using high temperature and high pressure method cooking process, first time
2.5h, second of 1.5h, the broken hectic fever due to yin of pig limbs bone is cooked into extract solution, because boiling pig bone extract solution is clear soup, the later stage passes through again
Condensation, centrifugation, ultrafiltration, freezing, again thawing, the refinement treatment process such as ultrafiltration are crossed, by process optimization, strand disconnects, and collects
More purpose things, improve yield, it is easy to which the impurity treatments such as macromolecule protein are removed.
Above-described is only presently preferred embodiments of the present invention, all timess made in the range of the spirit and principles in the present invention
What modifications, equivalent substitutions and improvements etc., should be included in the scope of the protection.
Claims (4)
1. a kind of extracting method of pig bone extract solution, it is characterised in that comprise the following steps：
Step 1：Pretreatment, pig bone is ground into after pretreatment broken bone；
Step 2：First extraction, broken bone is inserted in Rotating Stainless Steel Cage double-edged fine-toothed comb to the purified water for adding 1.5-2.5 times of broken bone weight, kept
130 ± 2 DEG C of hot pressing of temperature, Stress control extract boiling 2-3h, obtain first extract solution, carry for the first time in 0.2MPa ± 0.05MPa
Liquid is taken to be sent into cooling tank；
Step 3：Second extraction, the broken bone after first extraction is inserted in Rotating Stainless Steel Cage double-edged fine-toothed comb to the purifying for adding equivalent weight
Water, 130 ± 2 DEG C of hot pressing of keeping temperature, Stress control extract boiling 1-2h, obtain second extraction in 0.2MPa ± 0.05MPa
Liquid；
Step 4：First extract solution and secondary raffinate are merged, is post-processed to obtain and is less than 4000 dalton containing molecular weight
Peptide molecule chain pig bone extract solution.
2. the extracting method of pig bone extract solution according to claim 1, it is characterised in that described pig bone is the four limbs of pig
Bone.
3. the extracting method of pig bone extract solution according to claim 2, it is characterised in that the method tool of described pretreatment
Body is：Clean pig limbs bone and heat and boil, reject manadesma and residual meat, then crush.
4. the extracting method of pig bone extract solution according to claim 1, it is characterised in that the rear place in described step 4
The method of reason is：By the extract solution after merging by condensation, centrifugation, ultrafiltration, freezing, again thawing, ultrafiltration removal macromolecule protein
Impurity, obtain the pig bone extract solution for being less than the peptide molecule chain of 4000 dalton containing molecular weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710709940.1A CN107373677A (en) | 2017-08-18 | 2017-08-18 | A kind of extracting method of pig bone extract solution |
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| CN201710709940.1A CN107373677A (en) | 2017-08-18 | 2017-08-18 | A kind of extracting method of pig bone extract solution |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1831001A (en) * | 2005-03-08 | 2006-09-13 | 诺氏制药（吉林）有限公司 | Bone peptide extraction, prepn. method and use thereof |
| CN103843970A (en) * | 2012-11-30 | 2014-06-11 | 闫云霞 | Production method for preparing ossein oligopeptide meal, bone oil and bone meal |
-
2017
- 2017-08-18 CN CN201710709940.1A patent/CN107373677A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1831001A (en) * | 2005-03-08 | 2006-09-13 | 诺氏制药（吉林）有限公司 | Bone peptide extraction, prepn. method and use thereof |
| CN103843970A (en) * | 2012-11-30 | 2014-06-11 | 闫云霞 | Production method for preparing ossein oligopeptide meal, bone oil and bone meal |
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Application publication date: 20171124 |
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