CN107372820A - Goat milk method for disinfection - Google Patents
Goat milk method for disinfection Download PDFInfo
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- CN107372820A CN107372820A CN201710748935.1A CN201710748935A CN107372820A CN 107372820 A CN107372820 A CN 107372820A CN 201710748935 A CN201710748935 A CN 201710748935A CN 107372820 A CN107372820 A CN 107372820A
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- goat milk
- sterilization
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C7/00—Other dairy technology
- A23C7/02—Chemical cleaning of dairy apparatus; Use of sterilisation methods therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C3/00—Preservation of milk or milk preparations
- A23C3/08—Preservation of milk or milk preparations by addition of preservatives
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Abstract
The invention discloses a kind of goat milk method for disinfection, comprise the following steps：(1) goat milk is sterilized；(2) antibacterial substance is added to the goat milk after sterilization, be well mixed.Goat milk method for disinfection of the present invention, the widest thermal sterilization method for solving the problems, such as industrially to apply at present can cause damage serious while microorganism is killed to the nutritional ingredient in goat milk, flavor, color and luster etc., seek a kind of Ohmic heating method process for sterilizing that can reach pasteurize milk requirement, and compared with traditional thermal sterilization mode, embody the advantage of Ohmic heating method sterilization；The present invention has good researching value and promotional value, makes the Ohmic heating method sterilization technology of goat milk more ripe, produces good economic benefit；The nutritional ingredient that will not be destroyed in goat milk, goat milk will not be made to produce peculiar smell, and can ensure that goat milk keeps original color and luster and natural flavor.
Description
Technical field
The present invention relates to goat milk technical field of preservation of fresh, more particularly to a kind of goat milk method for disinfection.
Background technology
《Compendium of Materia Medica》In record " the sweet temperature of sheep milkiness, can mend cold empty weary, embellish lung, control and become thin, and treat consumptive disease, strengthening the essence gas,
And small intestine-QI, profit large intestine, cure infantile convulsion, retch gastric disorder causing nausea ", " milk cures mainly tonifying five zang organs, life people's fertilizer Bai Yueze, QI invigorating, control it is thin,
Happy skin, profit hair, eye droppings stop tear ".
Modern medicine and Nutritional studies show, sheep breast is to contain the nutrition beneficial to human body and each in mammal milk
Kind active material highest dairy products.Goat dairy contains more than 200 kinds of nutriment and bioactive substance, wherein containing a variety of breasts
Acid, 20 kinds of amino acid and 10 kinds of vitamin, 25 kinds of mineral matters, number enzymes.The lactose intolerances such as abdominal distension, diarrhoea will not be produced by drinking sheep breast
Disease, therefore sheep breast is the preferable dairy products selection of " lactose intolerance " person.Vitamin and have using sheep breast fed infant, in blood
The content of beneficial trace element is also all significantly higher than the baby of cow milk feeding, and its height, body weight, bone density and intelligence development speed are all
More than the baby of same period cow milk feeding.
Fresh goat milk can be polluted by dust, impurity, dirt and microorganism unavoidably, it is therefore necessary to by filtration, purification and
Sterilizing is bottled or could directly supply consumer after packing.
Number of patent application：201210182182.X discloses a kind of 100% liquid that can be preserved for a long time under normal temperature condition
The preparation method of pure goat milk.The invention passes through following steps：(1) 100% fresh sheep breast is taken, (2) are examined and cold chain, (3) filtering,
The net breast of centrifugation, (4) pasteurize and homogeneous, (5) ultra high temperature short time sterilization, (6) second homogenate, (7) de- to have a strong smell, ultra-high temperature sterilization,
(8) aseptic packaging, processing is carried out to fresh sheep breast and reaches the characteristic for removing the intrinsic smell of mutton of goat milk and goat milk, is solved existing
It is big that normal temperature liquid milk technical difficulty is processed into goat milk, it is impossible to the problem of the liquid deposited for a long time under normal temperature state pure goat milk.
Number of patent application：200610081141.6 propose a kind of method for including several sections of soft heating process, make first
Sheep breast temperature is slowly increased to 95 DEG C, through 200MPa pressure homogeneous, is again heated to 105 DEG C, and is kept for 33 seconds, is then heated to 115
DEG C, and kept for 4 seconds, 80-10 DEG C is finally rapidly decreased to, bacterium is not contained in so obtained goat milk, makes lactalbumin soft solidifying
Change, be easy to absorb.
The content of the invention
In view of many shortcomings of the prior art, the present inventor is paying sufficiently by substantial amounts of further investigation
After creative work, there is provided a kind of goat milk method for disinfection.
The present invention seeks to what is be achieved through the following technical solutions：
A kind of goat milk method for disinfection, comprises the following steps：
(1) goat milk is sterilized；
(2) antibacterial substance is added to the goat milk after sterilization, be well mixed.
A kind of goat milk method for disinfection, comprises the following steps：
(1) goat milk is subjected to Ohmic heating method sterilization；
(2) antibacterial substance is added to the goat milk after sterilization, be well mixed.
Preferably, the sterilization temperature that the Ohmic heating method is sterilized is 30-70 DEG C, sterilizing time 5-25s, voltage are
100-500V。
It is highly preferred that the sterilization temperature of the Ohmic heating method sterilization is 40-60 DEG C, sterilizing time 5-25s, voltage are
250-350V。
Preferably, a kind of goat milk method for disinfection, comprises the following steps：
(1) goat milk is subjected to Ohmic heating method sterilization；
(2) goat milk after Ohmic heating method is sterilized carries out microwave disinfection；
(3) antibacterial substance is added to the goat milk after sterilization, be well mixed.
Preferably, the power of the microwave disinfection is 500-900W, time 90-150s.
It is highly preferred that the power of the microwave disinfection is 700-800W, time 95-105s.
Preferably, the addition of the antibacterial substance is 50-500mg/L.
It is highly preferred that the addition of the antibacterial substance is 80-180mg/L.
Preferably, the antibacterial substance is alliin and/or gallic acid.It is highly preferred that the antibacterial substance is by 75-
85wt% alliins and 15-25wt% gallic acids composition.
Goat milk method for disinfection of the present invention, solve the widest thermal sterilization method industrially applied at present and killing
The nutritional ingredient in goat milk, flavor, color and luster etc. can be caused damage the problem of serious while microorganism, seeking one kind can reach
To the Ohmic heating method process for sterilizing of pasteurize milk requirement, and compared with traditional thermal sterilization mode, embody Ohmic heating method
The advantage of sterilization；The present invention has good researching value and promotional value, make the Ohmic heating method sterilization technology of goat milk more into
It is ripe, produce good economic benefit；The nutritional ingredient that will not be destroyed in goat milk, goat milk will not be made to produce peculiar smell, and can ensure that sheep
Milk keeps original color and luster and natural flavor.The present invention achieves excellent by using the sterilization of Ohmic heating method with reference to microwave sterilization
Effect, its reason is probably that microwave sterilizing effect is good, but nutritional ingredient is destroyed big；And Ohmic heating method bactericidal effect is poor,
But nutrient component damages are influenceed small.
Embodiment
Vitamin E and C method：
(1) experiment is prepared
Prepare 2% oxalic acid solution, ascorbic acid standard liquid and 2,6 dichlorophenol indophenol solution.
With ascorbic acid standard liquid demarcation 2,6 dichlorophenol indophenol titer before experiment.Take 1mL ascorbic acid standard liquids
In 50mL conical flasks, 10mL2% oxalic acid is added, shakes up, solution pinkiness 15s is titrated to not with 2,6- dichloroindophenol solution
Untill colour fading, 10mL2% oxalic acid is separately taken to do blank test, titer is calculated as follows：T=CV/ (V1-V2), wherein T are every
Milligram number of the milliliter 2,6 dichlorophenol indophenol solution equivalent to ascorbic acid；C be ascorbic acid concentration, mg/mL；V is anti-to draw
The volume of bad hematic acid, mL；V1 be titration ascorbic acid solution used in 2,6- dichloroindophenol solution volume, mL；V2 is empty for titration
The volume of Bai Suoyong 2,6- dichloroindophenol solution, mL.
(2) it is tested the titration of goat milk
10g goat milk is taken into 100mL volumetric flasks, adds after 2% oxalic acid solution shakes up in right amount and is settled to graduation mark again, take
In conical flask, calibrated 2, the 6- dichloroindophenol solution of use is titrated to solution pinkiness and keeps 15s not 10mL solution
Untill colour fading, while blank test is done, write down the amount of 2,6- dichloroindophenol solution used.Ascorbic content is calculated as follows：
R=(V-V0) TA/W, wherein R are Vitamin C content, (mg/100g)；V consumes 2,6 dichlorophenol indophenol to titrate during sample liquid
The volume of solution, mL；V0 consumes the volume of 2,6- dichloroindophenol solution, mL when being titration blank；T is 2,6 dichlorophenol indophenol solution
Titer, mg/mL；A is extension rate, the volume of pipette samples solution during cumulative volume/titration after sample solution constant volume；W
For example weight, g.
Method of protein measurement：
(1) drafting of standard curve is prepared
The standard protein solution of Coomassie brilliant G-250 protein reagent, 1mg/mL, six tool plug test tubes are taken, every is divided
Not Jia Ru standard protein solution 0,0.02,0.04,0.06,0.08,0.10mL, the distilled water of different volumes is added in every test tube
It is 1mL to make cumulative volume, and every test tube all adds 5mL Coomassie brilliant G-250 reagents, and Gai Saihou longitudinally falls solution in each test tube
Turn mixing, after placing 15 minutes under 595nm wavelength colorimetric, the absorbance A of each pipe measure is recorded, according to concentration and corresponding
Absorbance does standard curve, and standard curve regression equation is y=0.0045x+0.0353, R2=0.9879.
(2) protein content in goat milk is determined
Three 10mL tool plug test tubes are taken, each 0.1mL goat milk is added and 0.9mL distilled water, every test tube all adds 5mL and examined
Mas bright blue G-250 reagents, 15min is placed after fully mixing, the colorimetric under 595nm wavelength, record absorbance A, pass through mark
Directrix curve calculates protein content in goat milk.
Anti-microbial property test：Total number of bacteria：With reference to GB/T 4789.2-2010；Coliform：With reference to GB/T 4789.3-
2008。
Goat milk is the fresh goat milk of purebred Saanen goat production in embodiment.
Embodiment 1
Goat milk method for disinfection, comprises the following steps：
(1) goat milk is subjected to pasteurize, temperature is 85 DEG C, time 120s；
(2) alliin is added to the goat milk after sterilization, alliin addition is the 100mg/L (goat milk after i.e. every liter sterilization
Middle addition 100mg alliins), it is well mixed；Obtain sterilizing goat milk.
Nutrient component damages test result：Vitamin C loss is 13.6%, protein losses 2.3%；Wherein, dimension life
Goat milk vitamin C before plain C losses (%)=(goat milk Vitamin C content after goat milk Vitamin C content-sterilization before sterilization)/sterilization
Content × 100%, protein losses (%)=(sheep milk protein loss contains after sheep milk protein loss content-sterilization before sterilization
Amount)/sterilization before sheep milk protein loss content × 100%.
Sterilization goat milk is placed under 5 DEG C of temperature, the environment of relative humidity 50% after preserving 7 days and carries out total plate count, coliform
Group's test, test result are：Total plate count 3.2 × 104CFU/g, coliform 127MPN/100g.
Embodiment 2
Goat milk method for disinfection, comprises the following steps：
(1) goat milk is subjected to Ohmic heating method sterilization, sterilization temperature is 50 DEG C, sterilizing time 120s, voltage 300V；
(2) alliin is added to the goat milk after sterilization, alliin addition is the 100mg/L (goat milk after i.e. every liter sterilization
Middle addition 100mg alliins), it is well mixed；Obtain sterilizing goat milk.
Nutrient component damages test result：Vitamin C loss is 4.8%, protein losses 1.5%；Wherein, vitamin
Goat milk vitamin C contains before C loss (%)=(goat milk Vitamin C content after goat milk Vitamin C content-sterilization before sterilization)/sterilizations
Amount × 100%, protein losses (%)=(sheep milk protein loss contains after sheep milk protein loss content-sterilization before sterilization
Amount)/sterilization before sheep milk protein loss content × 100%.
Sterilization goat milk is placed under 5 DEG C of temperature, the environment of relative humidity 50% after preserving 7 days and carries out total plate count, coliform
Group's test, test result are：Total plate count 8620CFU/g, coliform 51MPN/100g.
Embodiment 3
Goat milk method for disinfection, comprises the following steps：
(1) goat milk is subjected to microwave disinfection, the power of microwave disinfection is 750W, time 120s；
(2) alliin is added to the goat milk after sterilization, alliin addition is the 100mg/L (goat milk after i.e. every liter sterilization
Middle addition 100mg alliins), it is well mixed；Obtain sterilizing goat milk.
Nutrient component damages test result：Vitamin C loss is 10.9%, protein losses 2.1%；Wherein, dimension life
Goat milk vitamin C before plain C losses (%)=(goat milk Vitamin C content after goat milk Vitamin C content-sterilization before sterilization)/sterilization
Content × 100%, protein losses (%)=(sheep milk protein loss contains after sheep milk protein loss content-sterilization before sterilization
Amount)/sterilization before sheep milk protein loss content × 100%.
Sterilization goat milk is placed under 5 DEG C of temperature, the environment of relative humidity 50% after preserving 7 days and carries out total plate count, coliform
Group's test, test result are：Total plate count 5870CFU/g, coliform 39MPN/100g.
Embodiment 4
Goat milk method for disinfection, comprises the following steps：
(1) goat milk is subjected to Ohmic heating method sterilization, sterilization temperature is 50 DEG C, sterilizing time 20s, voltage 300V；
(1) goat milk after Ohmic heating method is sterilized carries out microwave disinfection, and the power of microwave disinfection is 750W, and the time is
100s；
(2) alliin is added to the goat milk after microwave disinfection, alliin addition is 100mg/L (after i.e. every liter sterilization
100mg alliins are added in goat milk), it is well mixed；Obtain sterilizing goat milk.
Nutrient component damages test result：Vitamin C loss is 4.0%, protein losses 1.2%；Wherein, vitamin
Goat milk vitamin C contains before C loss (%)=(goat milk Vitamin C content after goat milk Vitamin C content-sterilization before sterilization)/sterilizations
Amount × 100%, protein losses (%)=(sheep milk protein loss contains after sheep milk protein loss content-sterilization before sterilization
Amount)/sterilization before sheep milk protein loss content × 100%.
Sterilization goat milk is placed under 5 DEG C of temperature, the environment of relative humidity 50% after preserving 7 days and carries out total plate count, coliform
Group's test, test result are：Total plate count 3040CFU/g, coliform 15MPN/100g.
Sterilization goat milk is placed under 5 DEG C of temperature, the environment of relative humidity 50% after preserving 7 days and carries out nutrient component damages survey
Examination, test result：Vitamin C loss is 1.8%, protein losses 0.7%；Wherein, vitamin C loss (%)=(preservation
Goat milk Vitamin C content after preceding goat milk Vitamin C content-preservation)/preserve before goat milk Vitamin C content × 100%, protein
Lose (%)=(sheep milk protein loss content after sheep milk protein loss content-preservation before preservation)/goat milk albumen before preserving
Matter loses content × 100%.
Embodiment 5
Goat milk method for disinfection, comprises the following steps：
(1) goat milk is subjected to Ohmic heating method sterilization, sterilization temperature is 50 DEG C, sterilizing time 20s, voltage 300V；
(1) goat milk after Ohmic heating method is sterilized carries out microwave disinfection, and the power of microwave disinfection is 750W, and the time is
100s；
(2) gallic acid is added to the goat milk after microwave disinfection, gallic acid addition is 100mg/L (i.e. every liter sterilization
100mg gallic acids are added in goat milk afterwards), it is well mixed；Obtain sterilizing goat milk.
Sterilization goat milk is placed under 5 DEG C of temperature, the environment of relative humidity 50% after preserving 7 days and carries out nutrient component damages survey
Examination, test result：Vitamin C loss is 2.5%, protein losses 0.8%；Wherein, vitamin C loss (%)=(preservation
Goat milk Vitamin C content after preceding goat milk Vitamin C content-preservation)/preserve before goat milk Vitamin C content × 100%, protein
Lose (%)=(sheep milk protein loss content after sheep milk protein loss content-preservation before preservation)/goat milk albumen before preserving
Matter loses content × 100%.
Sterilization goat milk is placed under 5 DEG C of temperature, the environment of relative humidity 50% after preserving 7 days and carries out total plate count, coliform
Group's test, test result：Total plate count 3920CFU/g, coliform 23MPN/100g.
Embodiment 6
Goat milk method for disinfection, comprises the following steps：
(1) goat milk is subjected to Ohmic heating method sterilization, sterilization temperature is 50 DEG C, sterilizing time 20s, voltage 300V；
(2) goat milk after Ohmic heating method is sterilized carries out microwave disinfection, and the power of microwave disinfection is 750W, and the time is
100s；
(3) gallic acid, alliin are added to the goat milk after microwave disinfection, gallic acid addition is 20mg/L (i.e. every
20mg gallic acids are added in goat milk after liter sterilization), alliin addition is that 80mg/L (adds in the goat milk after i.e. every liter sterilization
Enter 80mg alliins), it is well mixed；Obtain sterilizing goat milk.
Sterilization goat milk is placed under 5 DEG C of temperature, the environment of relative humidity 50% after preserving 7 days and carries out nutrient component damages survey
Examination, test result：Vitamin C loss is 1.2%, protein losses 0.4%；Wherein, vitamin C loss (%)=(preservation
Goat milk Vitamin C content after preceding goat milk Vitamin C content-preservation)/preserve before goat milk Vitamin C content × 100%, protein
Lose (%)=(sheep milk protein loss content after sheep milk protein loss content-preservation before preservation)/goat milk albumen before preserving
Matter loses content × 100%.
Sterilization goat milk is placed under 5 DEG C of temperature, the environment of relative humidity 50% after preserving 7 days and carries out total plate count, coliform
Group's test, test result：Total plate count 2160CFU/g, coliform 9MPN/100g.
Claims (10)
1. a kind of goat milk method for disinfection, it is characterised in that comprise the following steps：
(1) goat milk is sterilized；
(2) antibacterial substance is added to the goat milk after sterilization, be well mixed.
2. goat milk method for disinfection as claimed in claim 1, it is characterised in that comprise the following steps：
(1) goat milk is subjected to Ohmic heating method sterilization；
(2) antibacterial substance is added to the goat milk after sterilization, be well mixed.
3. goat milk method for disinfection as claimed in claim 2, it is characterised in that：The sterilization temperature of Ohmic heating method sterilization is
30-70 DEG C, sterilizing time 5-25s, voltage 100-500V.
4. goat milk method for disinfection as claimed in claim 3, it is characterised in that：The sterilization temperature of Ohmic heating method sterilization is
40-60 DEG C, sterilizing time 5-25s, voltage 250-350V.
5. goat milk method for disinfection as claimed in claim 2, it is characterised in that comprise the following steps：
(1) goat milk is subjected to Ohmic heating method sterilization；
(2) goat milk after Ohmic heating method is sterilized carries out microwave disinfection；
(3) antibacterial substance is added to the goat milk after sterilization, be well mixed.
6. goat milk method for disinfection as claimed in claim 5, it is characterised in that：The power of the microwave disinfection is 500-900W,
Time is 100-150s.
7. goat milk method for disinfection as claimed in claim 6, it is characterised in that：The power of the microwave disinfection is 700-800W,
Time is 95-105s.
8. the goat milk method for disinfection as described in claim 2 or 5, it is characterised in that：The addition of the antibacterial substance is 50-
500mg/L。
9. goat milk method for disinfection as claimed in claim 8, it is characterised in that：The addition of the antibacterial substance is 80-
180mg/L。
10. the goat milk method for disinfection as described in claim 2 or 5, it is characterised in that：The antibacterial substance be alliin and/or
Gallic acid.
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| CN201710748935.1A CN107372820B (en) | 2017-08-28 | 2017-08-28 | Goat milk sterilization method |
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| CN201710748935.1A CN107372820B (en) | 2017-08-28 | 2017-08-28 | Goat milk sterilization method |
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| CN107372820B CN107372820B (en) | 2020-03-10 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102599240A (en) * | 2012-03-07 | 2012-07-25 | 杨公明 | Method for processing fresh solid pasteurized milk |
| CN103181605A (en) * | 2013-03-08 | 2013-07-03 | 南京永青食品保鲜科技发展有限公司 | Sterilizing method and special microwave sterilization device for liquid food |
| CN203575567U (en) * | 2013-11-25 | 2014-05-07 | 厦门铖毅机电科技有限公司 | Ohmic heating sterilization system |
| CN105660850A (en) * | 2014-11-17 | 2016-06-15 | 张飞飞 | Cold sterilization method for removing goat milk smell |
-
2017
- 2017-08-28 CN CN201710748935.1A patent/CN107372820B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102599240A (en) * | 2012-03-07 | 2012-07-25 | 杨公明 | Method for processing fresh solid pasteurized milk |
| CN103181605A (en) * | 2013-03-08 | 2013-07-03 | 南京永青食品保鲜科技发展有限公司 | Sterilizing method and special microwave sterilization device for liquid food |
| CN203575567U (en) * | 2013-11-25 | 2014-05-07 | 厦门铖毅机电科技有限公司 | Ohmic heating sterilization system |
| CN105660850A (en) * | 2014-11-17 | 2016-06-15 | 张飞飞 | Cold sterilization method for removing goat milk smell |
Non-Patent Citations (1)
| Title |
|---|
| 高霞等: "欧姆加热对牛奶的杀菌效果及其营养成分的影响研究", 《山东农业大学学报(自然科学版)》 * |
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