CN1073720A - Automatic examination network system of pathogenic bacteria difficult of clinical routine examination and method - Google Patents
Automatic examination network system of pathogenic bacteria difficult of clinical routine examination and method Download PDFInfo
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- CN1073720A CN1073720A CN 91111898 CN91111898A CN1073720A CN 1073720 A CN1073720 A CN 1073720A CN 91111898 CN91111898 CN 91111898 CN 91111898 A CN91111898 A CN 91111898A CN 1073720 A CN1073720 A CN 1073720A
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Abstract
The present invention is a kind of network system and method to the self-verifying of routine clinical difficult check pathogenic bacteria.By user's sample preparation, communicating terminal, the sample transmission system, separation, culture systems, the analytical instrument system, unify communication system etc. of department of computer science constitutes network system to the self-verifying of routine clinical difficult check pathogenic bacteria, adopts user, transmission, inspection center, communication, sample preparation terminal to constitute the mode of supervising network, and use TCD in checkout procedure; The mode of FID and HIR coupling and R-netting fuzzy clustering algorithm are treated the sample product etc. hi-tech and are analyzed.That present method has is simple in structure, reliable, cost is low, go out that the result is fast, the service face width reaches advantages such as basic unit and rural area samll cities and towns.
Description
The invention belongs to and utilize scientific instrument, Medical Instruments routine clinical difficult a kind of method and the network system of checking pathogenic bacteria to do self-verifying, discipline range attribution analysis microbiology.
To the check of routine clinical difficult check pathogenic bacteria, all the someone did its a little researchs both at home and abroad.For example, present existing check anerobe basic skills is roughly three kinds: 1) the conventional bacterium inspection method of general hospital (comprising external).Use this method round of visits longer, needed 7-14 days just can obtain the result approximately, and inaccurate; 2) the Vitek identification card method of M-D company.This method cost height consumes greatly, and consumption is about 2.28 dollars/every card, and its identification card is that the said firm produces specially; 3) HP microorganism chromatograph.This is cover microorganism automatic checking system, i.e. the product 5898A bacteria analyzer of Hewlett-Packard of hewlette-packard development in 1985.This analyzer performance is good, but it is with high costs, its check is not to be foundation with VPI handbook (Anaerobe Laboratory manual.4th Edition Lillian V.Holdeman, et al.1977) data, the design of its no service network.In addition, to the check of tubercule bacillus, present employed method generally all needs just can obtain in 10～12 week result's (contain and do drug sensitive test).The special-purpose instrument of tubercule bacillus that detects fast of the up-to-date release of the U.S. also needs to detect in 4 week (containing the susceptibility test), and will use isotropic substance.
The objective of the invention is to, with the gas chromatography is core, Adama (Hadmard) method of infrared spectrophotometry that is aided with a particular section, with fast as far as possible speed, with the input of minimum (as equipment cost, operator and knowledge expertise level thereof, energy consumption spending etc.), to differentiate, to check the content of the feature short-chain fat acid constituents in the anerobe meta-bolites accurately, to determine which kind of anerobe whether has anerobe or determine in the submitted sample is; With short time check CO
2Productive rate, judge whether to exist tubercule bacillus; And the formation service network, serve a suitably interior user on a large scale, especially serve the rural area, such as each county hospital in a prefecture scope.
The said main points that routine clinical difficult check pathogenic bacteria is made the network method of self-verifying of the present invention are, chromatographic column by gas-chromatography, the FID(hydrogen flame ionization detector), HIR(Adama infrared analyzer), the TCD(thermal conductivity detector), computer and Computer Communication Networks terminal, existing telephone line (or telecommunication network), the network system that traffic, postal route, user's sample preparation terminal etc. constitute to the self-verifying of routine clinical difficult check pathogenic bacteria.
Utilize this network system to be to the method for anerobe check, after sample taking-up to be tested, a part of sample is made acidification immediately, another part sample is utilized immediately " user's sample preparation terminal " inject and have in the substratum of anaerobic environment, two portions sample is all inserted in the special container and is sent to the inspection center by traffic or postal route.In the inspection center above-mentioned two portions sample is done different processing respectively, part sample is after sampling user terminal (hospital) has been made acidification, promptly the specific meta-bolites " short chain fatty acid " of microorganism is fixed up, so that at the differing temps environment, do not change in the transportation of long period, after ether extraction is further made to this sample in the inspection center, entering chromatographic column immediately separates, is detected by FID, and by the computer real-time analysis, provide the result, in the shortest time, to determine whether to contain anaerobic infection; Another part is treated then to make esterification treatment through separation and Culture, the centrifugal supernatant liquor that obtains after graded samples is delivered to the inspection center, sends into hydrogen flame, optoelectronic detector that FID and HIR constitute, to whether having anerobe analysis, and determines the ownership of its anerobe.In the instrumental analysis system, have computer system based on the database of " VPI " handbook and special Infrared spectroscopy, utilize R-netting fuzzy clustering algorithm that sample is carried out fuzzy clustering, sample is compared, analyze its infrared signature with compiling method, so that differentiate and determine whether to exist anerobe and genus (genus) thereof and plant (specias); The result data that in computer, obtains, after modulation, demodulation, adopt man-to-man data double-way type of transmission, utilize the user terminal on telephone line (or radio communication mode) and the network system site to interrelate, at decoding terminals and after, promptly report in the end print result with the reinspection of signal backtrack test center.
Utilize this network system to be to the method for tubercule bacillus check, after sample taking-up to be tested, be inoculated on the tubercule bacillus substratum, and seal, sample can be placed in the special container that transports anerobe sample to be tested then, be sent to the inspection center by traffic or postal route with the anerobe check sample.In the inspection center, tubercule bacillus sample to be tested placed carry out the tubercule bacillus cultivation in the special-purpose incubator, through cultivating regularly inject gas chromatograph of the gas that produces, separate through vaporizing chamber, chromatographic column, detect by TCD, check CO
2Rate of rise, the gained signal is sent in the computer system, do data analysis through analog to digital conversion, quick identification software, judge whether to exist tubercule bacillus; The information that in computer, obtains, data, after modulation, decoding, also adopt man-to-man data double-way type of transmission, utilize the user terminal on telephone line (or radio communication mode) and the network system site to interrelate, at decoding terminals and after, promptly report at the user terminal print result with the reinspection of signal backtrack test center.
Adopt the method that routine clinical difficult check pathogenic bacteria is tested of the present invention, following advantage arranged:
(1) anerobe is checked:
1. the sample of obtaining to be tested is on sampling ground, promptly the user on the site (hospital) just carries out the first step acidification in network system, short chain fatty acid with the specific metabolite that guarantees fixation of microbe, and make in transportation, in the differing temps environment, do not change, also make simultaneously in the way that is reflected at the transportation sample after the first step acidification and finish, two combine into one to make the step of acidification and transportation;
2. sample is meant in anaerobic environment in the substratum of the anaerobic environment protection that sample is placed on gases formations such as nitrogen, carbonic acid gas, hydrogen, and special-purpose culture dish and the container of use, its culture dish and container can be put easily, sample thief and repeated use, anerobe can be preserved and cultivate effectively, and the time in the transportation can be made full use of;
3. it is fast to go out the result.The sample of part acidification through the simple analysis routine processes, can draw the report that whether exists anaerobism to infect in the inspection center in 1～2 hour;
4. owing to adopted R-netting fuzzy clustering algorithm, original needs are shortened to about 5 minutes 4 hours computing time; Make the calculating of anerobe classification reach quick.
5. adopt the analytical instrument system of Hadamard transform infrared analyzer and chromatographic column, fid detector coupling, and utilize Infrared spectroscopy and the VPI handbook computer for the database on basis is arranged, make device simpler, computing is easy, rapid, and can obtain good signal to noise ratio; Use method of the present invention can be in not wide infrared band (as only in the light wave scope of 3-6 mum wavelength) feature is proposed, through with the gas-chromatography hydrogen flame ionization detector in the signal Combined Processing that obtains, also can obtain not adding in the VPI handbook ownership of those bacterial classifications that biochemical method just can't distinguish;
(2) tubercule bacillus is checked:
To the check of tubercule bacillus, judgement can be from shortening to the time in 4 week (contain susceptibility test) with the required time in 10～12 week of prior art, but need not use isotropic substance.
(3) communication of the present invention is man-to-man data double-way transmission, utilize the user terminal on telephone line and telephone set (or radio communication) and the site to link, terminal have collect mail number, print result and foldback recheck signal and function such as sign for, simple in structure, reliable, cost is low.Can high-tech application in large-scale rural area, be had fabulous social benefit with cheaper cost.
Accompanying drawing 1, the automatic examination network system of pathogenic bacteria difficult of clinical routine examination schema.
In order to be illustrated more clearly in method of the present invention, be described below in conjunction with accompanying drawing:
The system that routine clinical difficult check pathogenic bacteria self-verifying method is adopted of the present invention mainly can be divided into the two parts, i.e. user's (I)-basic hospital inspection department, and inspection center (II).In user's (I), dispose devices such as user communication terminal and sample preparation terminal; In inspection center (II), dispose analytical instrument system (III) and computer system devices such as (IV).The workflow of the said method of inspection of the present invention in said system is:
After taking out sample to be tested (1) from the patient, to making the sample separated into two parts of anerobe check, a part is made acidification (2) (being described its flow process by dotted line in the accompanying drawing) as adding proper inorganic acid in the sample (about 1/2) of anerobe real-time analysis; Another part is made anaerobic treatment as the sample (about 1/2) of anerobe classification analysis through the sample preparation terminal of special use, and be inoculated in (3) (being described its flow process by fine rule in the accompanying drawing) on the substratum, then these samples are all placed special use to transport (6) in the container, be sent to inspection center (II) with the mode of postal route or traffic (7).After sample to be tested is received in the inspection center, will make lipid acid as a part of sample of anerobe real-time analysis and extract processing (8); To make separation and Culture and change PYG cultivation (9) with another part sample of determining the anerobe category attribution as the anerobe classification analysis, carry out esterification treatment (10) then; These samples of handling are respectively injected the chromatographic instrument vaporizing chamber (12) of analytical instrument system (III) respectively, with after chromatographic column is delivered to FID/HIR detector (14) after separating (13), to doing detecting of real-time analysis through FID; To doing then detecting through FID and HIR of multianalysis and definite its anerobe ownership, the detection signal that obtains is made electronics signal processing (15); Transmit signal to computer system then and carry out analog to digital conversion (16), signal after the analog to digital conversion is carried out data analysis (17), compare with " VPI " database and infrared data storehouse (18) simultaneously, analyze its infrared signature with R-netting fuzzy clustering algorithm, whether exist and determine its genus and kind so that differentiate anerobe; Output signal one tunnel after data analysis (17) carries out modulating/demodulating (19) after communication (21) mode and user communication terminal (22) networking; (20) are promptly write down, shown in another road of output signal for result's output, and this result can be for research, used when analyzing or checking.
Take out back (being described its flow process by heavy line in the accompanying drawing) to making tubercule bacillus sample to be tested (1), be inoculated in (4) on the tubercule bacillus substratum, and the special culture media device that will contain this substratum seals (5), also can place special use to transport (6) in the container then, be sent to inspection center's (II) with the mode of postal route or traffic (7).After this sample to be tested is received in the inspection center, proceed again that tubercule bacillus is cultivated and regularly in incubator extracting gases (11) the injecting chromatograph vaporizing chamber (12), through chromatographic separation (13), TCD detects (14), after the electronics signal processing (15), do data analysis by computer system (IV), determine whether to exist tubercle bacillus affection, which kind of tubercle bacillus affection (comprising drug sensitive test).
Adopt man-to-man data double-way formula type of transmission in inspection center's (II) between computer system (IV) and the user's (I), with the computer output signal after communication (wireless or liaison mode) circuit (21) is sent to user communication terminal (22), decode at user communication terminal, and also signal to be turned back to the inspection center recheck to confirm errorless after, promptly in user terminal print result report (23).
Of the present invention to said in the anerobe automatic testing method " R-netting " fuzzy clustering algorithm, its main points are: a) do the index property analysis according to the retention time of gas chromatogram (GC figure), determine the sample characteristics index, after spectrum peak height or peak area weighting, determine the span of sample index; B) size of index between more per two samples is listed n * n contingency table, asks the fuzzy similarity coefficient, lists fuzzy similarity matrix R; C) set up categorization levels α, α is cut matrix R
α, adopt R-netting method, sample is carried out fuzzy clustering.
In the said analytical instrument of the present invention system, can be provided with one or more check sample inlet, can carry out one or more sample analysis, processing simultaneously.In said computer system, can be provided with one or more interface, can provide service with one or more user communication terminal.
Be meant the sample that anerobe is checked at the said user terminal of the present invention place as sample preparation, to add mineral acid immediately in the about moiety sample that take out, make acidification, having preserved well so on the one hand that biological sample never degenerates during transporting, also is the first step of necessity of injection instrument pre-treatment sample on the other hand; After second half sample taking-up patient is external, promptly injects substratum and under the user terminal that the inspection center provided is supported, carry out the anaerobic environment protection immediately, charge into N in special transporting in the container (6)
2, CO
2, H
2Mixed gas shuts promptly to enter behind the lid and transports.Protected anerobe not caused death by airborne oxygen so first, the rapidity that the patient who makes remote user can enjoy city, place, inspection center basically can enjoy is cultivated in the time intercropping that also can utilize transport point to need in addition.The sample of doing the tubercule bacillus inspection then be need not anaerobic environment, be inoculated in immediately after sample takes out on the tubercule bacillus substratum (4) in the special-purpose incubator (5), seal up the mouth of incubator (5), can insert special use and transport transportation together in the container (6).
Claims (8)
1, a kind of by user's sample preparation, communicating terminal; The inspection center; Constitute with transmission, communication system etc., to the fast automatic supervising network system of routine clinical difficult check pathogenic bacteria, it utilizes this network system to method of anerobe check in the routine clinical difficult check pathogenic bacteria to be:
A, sample to be tested is made acidification immediately with a part of sample after taking out, another part is treated that graded samples reaches through the sample preparation terminal process of special use to inject and has in the anaerobic environment substratum, and two portions sample all places and is sent to the inspection center in the special container;
B does different processing respectively again with described two portions sample, and a part of sample is made ether extraction and gone into the analytical instrument system then and do real-time analysis, to determine whether to include anaerobic infection; Whether another part treats that graded samples then through separation and Culture and after changeing PYG and cultivating, makes esterification treatment with centrifuged supernatant, reenter its anerobe of analytical instrument systems analysis and exist, and determine ownership;
C treats the sample of quick test, enter instrument after, through separate, FID detects, the gained signal is sent in the computer, through the fast coding software analysis, can determine whether this sample contains anaerobic infection; After treating that the partition test sample enters instrument, detect through separation, FID, after HIR detects, detection signal is sent in the computer that has based on the database of " VPI " handbook and ir data storehouse, utilize " R-netting " fuzzy clustering algorithm to carry out fuzzy clustering, compare with " VPI " database, analyze its infrared signature, so that differentiate whether anerobe exists and determine its genus and kind with compiling method;
D, after from computer, obtaining a result, utilize the user communication terminal on telephone line (or radio communication mode) and the network system site to adopt man-to-man data double-way type of transmission to carry out communication, at decoding terminals and with the reinspection of signal backtrack test center and after both sides sign for, promptly report in the end print result.
2, the method to the anerobe check according to claim 1, it is characterized in that said analytical instrument system includes the optoelectronic detector that gas chromatographic column, hydrogen flame ionization detector (being FID) and Hadamard transform infrared analyzer (being HIR) constitute; Electronics signal processor etc.; Said computer system includes the ir data storehouse that has " VPI " database and special use, R-netting method, quick identification software, coding and decoding system, the computerized identification system of modulation, detuner etc.
3, the method to anerobe check according to claim 2 is characterized in that, the discrimination method that said computer adopts is " R-netting " fuzzy clustering algorithm, and its main points are,
A does the index property analysis according to the retention time of gas chromatogram, determines the sample characteristics index, after spectrum peak height or peak area weighting, establishes sample index's span;
B, the size of more per two sample room indexs is listed n * n contingency table, asks fuzzy similarity matrix R;
C sets up categorization levels α, and α is cut matrix R
α, adopt R-netting method, sample is carried out fuzzy clustering.
4, the method to the anerobe check according to claim 3 is characterized in that, said a part of sample is meant as acidification immediately adds mineral acid in sample, and this acidification is placed in the network system carries out in the user on the site (hospital); Said another part treats that graded samples injects in the culture dish and special container with the anaerobic environment substratum that is meant in the anaerobic environment substratum that gases such as sample being placed on nitrogen, carbonic acid gas, hydrogen constitute immediately.
5, the method to anerobe check according to claim 4 is characterized in that, can be provided with one or more check sample inlet in the said analytical instrument system, can do analysis, the processing of one or more sample to be tested simultaneously; Can be provided with one or more interface in the said computer system, can connect the service that provides with one or more user communication terminal.
6, a kind of by user's sample preparation, communicating terminal; The inspection center; The fast automatic supervising network system that transmission, communication system etc. constitute to routine clinical difficult check pathogenic bacteria, it utilizes this network system to method of tubercule bacillus check in the routine clinical difficult check pathogenic bacteria to be:
A after sample to be tested takes out, is inoculated on the tubercule bacillus substratum in the special-purpose incubator, and seals, and places then and is sent to the inspection center in the special container;
B inserts sample to be tested and carries out the tubercule bacillus cultivation in the special-purpose incubator, regularly send gas in the incubator to enter the analytical instrument system then and analyzes;
C after sample gas to be tested enters analytical instrument, separates the TCD(thermal conductance through gas chromatograph vaporizing chamber, chromatographic column) detector, provide electrical signal, realize checking CO with chromatographic process
2Rate of rise; The gained signal is sent in the computer system, after analog to digital conversion, data analysis, judged whether to exist tubercule bacillus, be which kind of tubercule bacillus.(comprise and do drug sensitive test)
D, after from computer, obtaining a result, utilize the user communication terminal on telephone line (or radio communication mode) and the network system site to adopt man-to-man data double-way type of transmission to carry out communication, at decoding terminals and with the reinspection of signal backtrack test center and after both sides sign for, promptly report in the end print result.
7, the method to the tubercule bacillus check according to claim 6 is characterized in that, in the sample cultivation to be tested, regularly with the sample gas inject gas chromatograph vaporizing chamber in the incubator.
8, the method to the tubercule bacillus check according to claim 7 is characterized in that said analytical instrument comprises gas chromatographic column; Thermal conductivity detector (being TCD); Electronics letter treater etc.; Said computer system includes analog to digital conversion, quick identification software, modulation, detuner etc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 91111898 CN1064712C (en) | 1991-12-23 | 1991-12-23 | Automatic examination network system of pathogenic bacteria difficult of clinical routine examination and its method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 91111898 CN1064712C (en) | 1991-12-23 | 1991-12-23 | Automatic examination network system of pathogenic bacteria difficult of clinical routine examination and its method |
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| Publication Number | Publication Date |
|---|---|
| CN1073720A true CN1073720A (en) | 1993-06-30 |
| CN1064712C CN1064712C (en) | 2001-04-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 91111898 Expired - Fee Related CN1064712C (en) | 1991-12-23 | 1991-12-23 | Automatic examination network system of pathogenic bacteria difficult of clinical routine examination and its method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994029480A1 (en) * | 1993-06-14 | 1994-12-22 | Lin He | A bacteria identification method and apparatus |
| CN107223248A (en) * | 2015-02-13 | 2017-09-29 | 利物浦大学 | For the method and apparatus for the grader for creating the presence for indicating medical conditions |
-
1991
- 1991-12-23 CN CN 91111898 patent/CN1064712C/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994029480A1 (en) * | 1993-06-14 | 1994-12-22 | Lin He | A bacteria identification method and apparatus |
| US5783410A (en) * | 1993-06-14 | 1998-07-21 | Lin He | Bacteria identification method and apparatus |
| CN107223248A (en) * | 2015-02-13 | 2017-09-29 | 利物浦大学 | For the method and apparatus for the grader for creating the presence for indicating medical conditions |
| CN107223248B (en) * | 2015-02-13 | 2021-11-02 | 利物浦大学 | Method and apparatus for creating a classifier indicative of the presence of a medical condition |
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| Publication number | Publication date |
|---|---|
| CN1064712C (en) | 2001-04-18 |
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