CN107356657A - A kind of preparation of chitosan nanoparticles and its application in Detecting Pesticide - Google Patents
A kind of preparation of chitosan nanoparticles and its application in Detecting Pesticide Download PDFInfo
- Publication number
- CN107356657A CN107356657A CN201710565789.9A CN201710565789A CN107356657A CN 107356657 A CN107356657 A CN 107356657A CN 201710565789 A CN201710565789 A CN 201710565789A CN 107356657 A CN107356657 A CN 107356657A
- Authority
- CN
- China
- Prior art keywords
- chitosan
- preparation
- chitosan nanoparticles
- parts
- minutes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage
Abstract
The invention discloses a kind of preparation of chitosan nanoparticles of technical field of analysis and detection and its application in Detecting Pesticide, the preparation method of the chitosan nanoparticles to comprise the following steps：S1.1：12 parts of chitosan is incorporated in 120 140 parts of plasma water, then add glacial acetic acid solution so that the concentration of glacial acetic acid is 1% 1.4%；S1.2：Solution in step S1 will be stirred to abundant dissolving；S1.3：Using ultrasonic oscillation 38 minutes, centrifuge 20 30 minutes, abandoning supernatant, freezing, dry, preparation method is simple, simplifies the preparation process of chitosan nanoparticles, reduce production cost, and the chitosan nanoparticles stability produced is good, the detection to residues of pesticides is carried out using chitosan nanoparticles, improves accuracy of detection, and brought great convenience to testing staff, realize pesticide multi-residues on-line quick detection.
Description
Technical field
The present invention relates to technical field of analysis and detection, the preparation of specially a kind of chitosan nanoparticles and its residual in agricultural chemicals
Stay the application in detection.
Background technology
Chitosan is also known as chitosan, is that the chitin being widely present by nature obtains by deacetylation
, chemical name is Chitosan (1-4) -2- amino-B-D glucose.From 1859, Frenchman Rouget obtained shell first
After glycan, the biological functionality and compatibility of this natural polymer, blood compatibility, security, microbic resolvability etc. are excellent
It is benign can by all trades and professions extensive concern, medicine, food, chemical industry, cosmetics, water process, METAL EXTRACTION and recovery, biochemistry and
The application study of the numerous areas such as biomedical engineering achieves major progress.Result of study shows that the particle diameter of chitosan is diminishing
When, chitosan action effect is better, and the preparation method of existing chitosan nanoparticles is complicated, and cost is too high, Er Qienong
Medicine inspection is surveyed complex for operation step, and accuracy of detection is low.A kind of therefore, it is proposed that preparation of chitosan nanoparticles and its in agriculture
Application in medicine residue detection.
The content of the invention
Answering it is an object of the invention to provide a kind of preparation of chitosan nanoparticles and its in Detecting Pesticide
With, to solve the preparation method of the existing chitosan nanoparticles proposed in above-mentioned background technology complexity, cost is too high, and
Complex for operation step, the problem of accuracy of detection is low of Pesticides Testing.
To achieve the above object, the present invention provides following technical scheme：A kind of preparation method of chitosan nanoparticles, should
The preparation method of chitosan nanoparticles comprises the following steps：
S1.1：The chitosan of 1-2 parts is incorporated in the plasma water of 120-140 parts, then adds glacial acetic acid solution so that
The concentration of glacial acetic acid is 1%-1.4%；
S1.2：Solution in step S1 will be stirred to abundant dissolving；
S1.3：Using ultrasonic oscillation 3-8 minutes, 20-30 minutes are centrifuged, abandoning supernatant, are freezed, are dried.
Preferably, the stir speed (S.S.) in the step S1.2 is 250-280 revs/min, and mixing time is 10-14 hours.
Preferably, the ultrasonic power in the step S1.3 is 80-140W.
Preferably, the centrifugation rate in the step S1.3 is 11000-15000 revs/min.
Preferably, application process of a kind of chitosan nanoparticles in Detecting Pesticide, the chitosan nanoparticles
Application process in Detecting Pesticide comprises the following steps：
S2.1：3 parts of agricultural product are taken, are added in 120 parts of deionized water, are crushed, add acetonitrile-acetone solution centrifugal, extraction
Take；
S2.2：It is put into using stirring rod of the surface coated with chitosan-graphite ene coatings in the extract in step S2.1,
It is stirred by the way of magnetic agitation, then carries out SPE；
S2.3：Using cyclic voltammetry curve method, Electrochemical Detection is carried out.
Preferably, the magnetic agitation time in the step S2.2 is 20-30 minutes.
Compared with prior art, the beneficial effects of the invention are as follows：A kind of system for chitosan nanoparticles that the invention proposes
The standby and its application in Detecting Pesticide, preparation method is simple, simplifies the preparation process of chitosan nanoparticles, reduces
Production cost, and the chitosan nanoparticles stability produced is good, carried out using chitosan nanoparticles residual to agricultural chemicals
The detection stayed, accuracy of detection is improved, and brought great convenience to testing staff, realize that pesticide multi-residues are quickly examined online
Survey.
Embodiment
The technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described implementation
Example only part of the embodiment of the present invention, rather than whole embodiments.It is common based on the embodiment in the present invention, this area
The every other embodiment that technical staff is obtained under the premise of creative work is not made, belong to the model that the present invention protects
Enclose.
The present invention provides a kind of preparation method of chitosan nanoparticles：
Embodiment 1
The preparation method of the chitosan nanoparticles comprises the following steps：
S1.1：1 part of chitosan is incorporated in 120 parts of plasma water, then add glacial acetic acid solution so that glacial acetic acid
Concentration be 1%；
S1.2：Solution in step S1 will be stirred to abundant dissolving, stir speed (S.S.) is 250 revs/min, mixing time 10
Hour；
S1.3：Using ultrasonic oscillation 3 minutes, ultrasonic power 80W, centrifuging 20 minutes, centrifugation rate is 11000 turns/
Point, abandoning supernatant, freeze, dry.
Embodiment 2
The preparation method of the chitosan nanoparticles comprises the following steps：
S1.1：2 parts of chitosan is incorporated in 140 parts of plasma water, then add glacial acetic acid solution so that glacial acetic acid
Concentration be 1.4%；
S1.2：Solution in step S1 will be stirred to abundant dissolving, stir speed (S.S.) is 280 revs/min, mixing time 14
Hour；
S1.3：Using ultrasonic oscillation 8 minutes, ultrasonic power 140W, centrifuging 30 minutes, centrifugation rate is 15000 turns/
Point, abandoning supernatant, freeze, dry.
Embodiment 3
The preparation method of the chitosan nanoparticles comprises the following steps：
S1.1：1.5 parts of chitosan is incorporated in 130 parts of plasma water, then add glacial acetic acid solution so that ice vinegar
The concentration of acid is 1.2%；
S1.2：Solution in step S1 will be stirred to abundant dissolving, stir speed (S.S.) is 265 revs/min, mixing time 12
Hour；
S1.3：Using ultrasonic oscillation 5 minutes, ultrasonic power 110W, centrifuging 25 minutes, centrifugation rate is 13000 turns/
Point, abandoning supernatant, freeze, dry.
The present invention also provides a kind of application process of chitosan nanoparticles in Detecting Pesticide, the chitosan nano
Application process of the particulate in Detecting Pesticide comprises the following steps：
S2.1：3 parts of agricultural product are taken, are added in 120 parts of deionized water, are crushed, add acetonitrile-acetone solution centrifugal, extraction
Take；
S2.2：It is put into using stirring rod of the surface coated with chitosan-graphite ene coatings in the extract in step S2.1,
It is stirred by the way of magnetic agitation, the magnetic agitation time is 20-30 minutes, then carries out SPE；
S2.3：Using cyclic voltammetry curve method, Electrochemical Detection is carried out.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of changes, modification can be carried out to these embodiments, replace without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (6)
- A kind of 1. preparation method of chitosan nanoparticles, it is characterised in that：The preparation method of the chitosan nanoparticles includes Following steps：S1.1：The chitosan of 1-2 parts is incorporated in the plasma water of 120-140 parts, then add glacial acetic acid solution so that ice vinegar The concentration of acid is 1%-1.4%；S1.2：Solution in step S1 will be stirred to abundant dissolving；S1.3：Using ultrasonic oscillation 3-8 minutes, 20-30 minutes are centrifuged, abandoning supernatant, are freezed, are dried.
- A kind of 2. preparation method of chitosan nanoparticles according to claim 1, it is characterised in that：The step S1.2 In stir speed (S.S.) be 250-280 revs/min, mixing time is 10-14 hours.
- A kind of 3. preparation method of chitosan nanoparticles according to claim 1, it is characterised in that：The step S1.3 In ultrasonic power be 80-140W.
- A kind of 4. preparation method of chitosan nanoparticles according to claim 1, it is characterised in that：The step S1.3 In centrifugation rate be 11000-15000 revs/min.
- A kind of 5. application process of chitosan nanoparticles in Detecting Pesticide, it is characterised in that：The chitosan nano is micro- Application process of the grain in Detecting Pesticide comprises the following steps：S2.1：3 parts of agricultural product are taken, are added in 120 parts of deionized water, are crushed, add acetonitrile-acetone solution centrifugal, extraction；S2.2：It is put into the extract in step S2.1, is used using stirring rod of the surface coated with chitosan-graphite ene coatings The mode of magnetic agitation is stirred, and then carries out SPE；S2.3：Using cyclic voltammetry curve method, Electrochemical Detection is carried out.
- 6. a kind of application process of the chitosan nanoparticles according to claim 5 in Detecting Pesticide, its feature It is：The magnetic agitation time in the step S2.2 is 20-30 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710565789.9A CN107356657A (en) | 2017-07-12 | 2017-07-12 | A kind of preparation of chitosan nanoparticles and its application in Detecting Pesticide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710565789.9A CN107356657A (en) | 2017-07-12 | 2017-07-12 | A kind of preparation of chitosan nanoparticles and its application in Detecting Pesticide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107356657A true CN107356657A (en) | 2017-11-17 |
Family
ID=60292302
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710565789.9A Pending CN107356657A (en) | 2017-07-12 | 2017-07-12 | A kind of preparation of chitosan nanoparticles and its application in Detecting Pesticide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107356657A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109270228A (en) * | 2018-11-24 | 2019-01-25 | 广西中医药大学附属瑞康医院 | A kind of Monitoring Pesticide Residues method and system based on graphene sensor |
| CN109655365A (en) * | 2018-12-29 | 2019-04-19 | 江苏微谱检测技术有限公司 | The detection method of organic agricultural chemicals in a kind of soil |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1237988A1 (en) * | 1999-10-29 | 2002-09-11 | Cognis Deutschland GmbH | Method for producing nanoparticulate chitosans or chitosan derivatives |
| CN101153081A (en) * | 2006-09-26 | 2008-04-02 | 香港理工大学 | Method of producing chitosan nano grain |
| CN101633739A (en) * | 2008-07-24 | 2010-01-27 | 财团法人食品工业发展研究所 | Method for preparing chitosan nano-particles in water phase |
| CN105037582A (en) * | 2015-06-10 | 2015-11-11 | 山东安特生物技术有限公司 | Preparation method and application of nano chitosan |
| CN105079878A (en) * | 2015-09-14 | 2015-11-25 | 江南大学 | Preparation method of nano antibacterial coating based on chitosan complex micelle |
| CN105241981A (en) * | 2015-11-23 | 2016-01-13 | 袁晓林 | Method for measuring pesticide residue amount in tea leaves |
-
2017
- 2017-07-12 CN CN201710565789.9A patent/CN107356657A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1237988A1 (en) * | 1999-10-29 | 2002-09-11 | Cognis Deutschland GmbH | Method for producing nanoparticulate chitosans or chitosan derivatives |
| CN101153081A (en) * | 2006-09-26 | 2008-04-02 | 香港理工大学 | Method of producing chitosan nano grain |
| CN101633739A (en) * | 2008-07-24 | 2010-01-27 | 财团法人食品工业发展研究所 | Method for preparing chitosan nano-particles in water phase |
| CN105037582A (en) * | 2015-06-10 | 2015-11-11 | 山东安特生物技术有限公司 | Preparation method and application of nano chitosan |
| CN105079878A (en) * | 2015-09-14 | 2015-11-25 | 江南大学 | Preparation method of nano antibacterial coating based on chitosan complex micelle |
| CN105241981A (en) * | 2015-11-23 | 2016-01-13 | 袁晓林 | Method for measuring pesticide residue amount in tea leaves |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109270228A (en) * | 2018-11-24 | 2019-01-25 | 广西中医药大学附属瑞康医院 | A kind of Monitoring Pesticide Residues method and system based on graphene sensor |
| CN109655365A (en) * | 2018-12-29 | 2019-04-19 | 江苏微谱检测技术有限公司 | The detection method of organic agricultural chemicals in a kind of soil |
| CN109655365B (en) * | 2018-12-29 | 2021-03-12 | 江苏微谱检测技术有限公司 | Method for detecting organic pesticide in soil |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zeng et al. | A review on peach gum polysaccharide: Hydrolysis, structure, properties and applications | |
| e Silva et al. | Microwave-assisted extraction of polysaccharides from Arthrospira (Spirulina) platensis using the concept of green chemistry | |
| Liu et al. | α-Glucosidase immobilization on chitosan-enriched magnetic composites for enzyme inhibitors screening | |
| Hossain et al. | Production and characterization of chitosan from shrimp waste | |
| CN106146687B (en) | A kind of method of pectin in extraction citrus peel residue | |
| Bacon et al. | The separation of β-glucanases produced by Cytophaga johnsonii and their role in the lysis of yeast cell walls | |
| CN105906741B (en) | A method of extracting pectin from discarded sisal dregs | |
| CN107356657A (en) | A kind of preparation of chitosan nanoparticles and its application in Detecting Pesticide | |
| CN104231103B (en) | Flammulina velutipes polysaccharide as well as preparation method and application thereof | |
| CN105177083A (en) | Method for improving enzymolysis efficiency of lignocellulose biomass | |
| Poommarinvarakul et al. | Isolation and rheological properties of tamarind seed polysaccharide from tamarind kernel powder using protease enzyme and high‐intensity ultrasound | |
| CN104211828A (en) | Mulberry anti-oxidation polysaccharides and preparation method thereof | |
| CN109603771A (en) | A kind of preparation method of chitosan magnetic-montmorillonite-based nano complex microsphere | |
| CN105348165A (en) | Method for extracting astaxanthin yeast, yeast extract and glucan from yeast | |
| Yusof et al. | Characterization of pectin extracted from guava peels using deep eutectic solvent and citric acid | |
| CN103271835B (en) | Silk fibroin nanocrystalline and preparation method thereof | |
| CN106749684A (en) | A kind of malic acid is esterified the preparation method of nano-cellulose | |
| CN105622778A (en) | Preparation method of water-soluble chitosan | |
| CN108314748A (en) | A kind of aloe skin pectous extracting method | |
| CN112175098A (en) | Method for efficiently preparing polysaccharide from sparassis crispa stipe | |
| CN105693885A (en) | Method for extracting natural component chitin nanocrystal of edible fungi | |
| US10093952B2 (en) | Method for preparing yeast beta-D-glucan using solubilization technology based on molecular assembly | |
| CN104558231A (en) | Leaching extraction method of plant polysaccharide | |
| CN103881725B (en) | Method for preparing tree frog skin freeze-dried powder | |
| CN106368027A (en) | Method for preparing nanocellulose through oxidative degradation of ammonium persulfate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171117 |