CN107354085B

CN107354085B - Lifting structure, temperature metal bath and method for realizing temperature difference

Info

Publication number: CN107354085B
Application number: CN201710664831.2A
Authority: CN
Inventors: 周中人; 姜杰; 汪金石
Original assignee: Shanghai Kinbio Tech Co ltd
Current assignee: Shanghai Kinbio Tech Co ltd
Priority date: 2017-08-07
Filing date: 2017-08-07
Publication date: 2023-09-08
Anticipated expiration: 2037-08-07
Also published as: CN107354085A

Abstract

The invention discloses a lifting structure, a temperature metal bath and a method for realizing temperature difference, wherein a rotating shaft body and a lifting assembly are arranged, and the rotating shaft body is matched with the lifting assembly to realize the lifting of the rotating shaft body; when the rotation shaft body rotates in one direction, the rotation shaft body will rise a certain distance and rotate with the rotation shaft body, and when the rotation shaft body rotates in the opposite direction, the rotation shaft body will fall a certain distance and rotate with the rotation shaft body. The lifting structure and the temperature metal bath with the lifting structure provided by the invention realize differential temperature heating by moving the reaction container, so that the high and low temperature switching in the PCR nucleic acid amplification reaction process is realized more quickly, and the differential temperature heating by moving the reaction container has obvious speed advantage compared with the temperature lifting by a semiconductor.

Description

Lifting structure, temperature metal bath and method for realizing temperature difference

Technical Field

The invention relates to the technical field of chemical experiment equipment, in particular to a lifting structure, a temperature metal bath with the lifting structure and a method for realizing temperature difference.

Background

In recent years, with the rapid development of science and technology, the fields of chemistry and biology are also continuously advancing, and experiments are an important part; PCR (polymerase chain reaction) is an important technology in the fields of life sciences and medicine, and is used for amplifying a large amount of target DNA, thereby further performing subsequent research work. The appearance of the PCR instrument realizes the automation of the technology, so that the technology of the PCR is widely applied, such as diagnosis of hereditary diseases, detection of nucleic acid of pathogens in clinical specimens, genetic identification of forensic specimens, analysis of mutation conditions in activated oncogenes and the like, and the main principle is that the temperature is increased to denature DNA, then the temperature is reduced to anneal and extend the DNA, and the temperature is precisely controlled in the process. In the PCR (polymerase chain reaction) in the test, DNA becomes single-stranded by utilizing the high temperature time variability of 95 ℃ in vitro, the primer and the single-stranded are combined according to the base complementary pairing principle at low temperature (usually about 60 ℃), the temperature is adjusted to the optimal reaction temperature (about 72 ℃) of DNA polymerase, and the DNA polymerase synthesizes the complementary strand along the direction from phosphoric acid to five-carbon sugar (5 '-3'). The PCR instrument based on polymerase is actually a temperature control device, and can well control the denaturation temperature, the renaturation temperature and the extension temperature; therefore, the technology is automated by the appearance of a PCR instrument, so that the technology of PCR is widely applied, such as diagnosis of genetic diseases, detection of nucleic acid of pathogens in clinical specimens, genetic identification of forensic specimens, analysis of mutation conditions in activated oncogenes and the like; in view of the semiconductor materials limitations, the current temperature rise and fall rates of PCR instruments are about 3-6 degrees per second, while the temperature difference of 55-95 degrees for PCR reactions is 40 degrees, so the fastest temperature change time will be about 7 seconds. Most of the current PCR instruments are not provided with a circulating temperature controller, are not sensitive enough to control the temperature, and influence the replication and detection speed of genes.

Disclosure of Invention

This section is intended to outline some aspects of embodiments of the application and to briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section as well as in the description of the application and in the title of the application, which may not be used to limit the scope of the application.

The present application has been made in view of the above and/or problems occurring in the prior art lifting structures and temperature metal baths with lifting structures.

It is therefore one of the objects of the present application to provide a lifting structure.

In order to solve the technical problems, the application provides the following technical scheme: the lifting structure comprises a rotating shaft body and a lifting assembly, wherein the rotating shaft body is matched with the lifting assembly to realize the lifting of the rotating shaft body; when the rotating shaft body rotates in one direction, the rotating shaft body is lifted by a certain distance; when the rotating shaft body rotates in the opposite direction, the rotating shaft body is lowered by a certain distance; the rotating shaft body is matched with the lifting assembly to realize lifting of the rotating shaft body;

when the rotating shaft body rotates in one direction, the rotating shaft body is lifted by a certain distance; when the rotating shaft body rotates in the opposite direction, the rotating shaft body is lowered by a certain distance;

The rotary shaft body comprises a first shaft body and a second shaft body, and the first shaft body and the second shaft body are matched up and down; the lifting assembly comprises a shaft sleeve part and a protruding piece,

the shaft sleeve part is of a hollow structure and is arranged at the upper end of the first shaft body, the second shaft body rotates in the shaft sleeve part, and the shaft sleeve part is provided with an upward-inclined conversion channel;

the protruding piece is arranged on the second shaft body in a protruding shape and is matched with the conversion channel;

a temperature control part capable of forming a first temperature zone in which a temperature is outputted according to a set value;

and the fixing component is arranged on the lifting structure, is used for fixing the target reaction container and can generate relative distance change along with the lifting change of the lifting structure and the temperature control component, so as to heat the reaction container at different temperatures.

As a preferable mode of the elevating structure of the present invention, wherein: the lifting assembly further comprises a fixed supporting part, wherein the fixed supporting part is arranged on the second shaft body, the diameter of the fixed supporting part is larger than that of the second shaft body, and a one-way bearing is arranged at the lower end of the fixed supporting part; the damping component is arranged at the joint of the fixed supporting part and the one-way bearing, and the damping component can generate damping action on the lower ends of the one-way bearing and the fixed supporting part.

As a preferred embodiment of the temperature metal bath with a lifting structure according to the invention, wherein: the damping part adopts a rubber wear-resistant ring.

As a preferred embodiment of the temperature metal bath with a lifting structure according to the invention, wherein: the damping part adopts a plug.

As a preferable mode of the elevating structure of the present invention, wherein: the maximum width of the fixed supporting part is the same as the width of the one-way bearing, so that the fixed supporting part and the one-way bearing can be contacted with the damping component.

It is another object of the present invention to provide a temperature metal bath with a lifting structure.

The invention provides the following technical scheme: a temperature metal bath with a lifting structure includes a temperature control part capable of forming a first temperature zone for outputting a temperature according to a set value; a lifting structure; and the fixing component is arranged on the lifting structure, is used for fixing the target reaction container and can generate relative distance change along with the lifting change of the lifting structure and the temperature control component, so as to heat the reaction container at different temperatures.

As a preferred embodiment of the temperature metal bath with a lifting structure according to the invention, wherein: the temperature control component comprises a first temperature control module for controlling the temperature change of the first heating device; and the first heating device is connected with the first temperature control module and used for heating the reaction container at different temperatures through temperature change.

As a preferred embodiment of the temperature metal bath with a lifting structure according to the invention, wherein: the temperature control member further includes a heat transfer material disposed on the first heating device, a first temperature zone being formed by the heat transfer material, and the reaction vessel being heated in contact with the heat transfer material.

As a preferred embodiment of the temperature metal bath with a lifting structure according to the invention, wherein: the reactor also comprises a first solution zone and a second solution zone which are arranged on the reaction container, and the first solution zone passes through the first temperature zone by rotating.

As a preferred embodiment of the temperature metal bath with a lifting structure according to the invention, wherein: the reaction vessel further comprises a second temperature zone, and the first solution zone of the reaction vessel passes through the first temperature zone and the second temperature zone in sequence through rotation.

As a preferred embodiment of the temperature metal bath with a lifting structure according to the invention, wherein: and a second temperature zone through which the first solution zone and the second solution zone of the reaction vessel pass, respectively, by rotating.

As a preferred embodiment of the temperature metal bath with a lifting structure according to the invention, wherein: the temperature control part further comprises a third temperature zone and a fourth temperature zone, and the second solution zone of the reaction vessel passes through the third temperature zone and the fourth temperature zone respectively by rotating.

As a preferred embodiment of the temperature metal bath with a lifting structure according to the invention, wherein: the temperature control component comprises a second temperature control module, a first heating device and a second heating device, wherein the second temperature control module is used for controlling the second heating device to output stable temperature; and the second heating device is connected with the second temperature control module, can be close to or far away from the reaction container, and is used for heating the reaction container at different temperatures through distance change.

As a preferred embodiment of the temperature metal bath with a lifting structure according to the invention, wherein: the lifting structure further comprises a holding part which is of a hollow structure, the upper end of the holding part is used for fixing the damping part, and the lower end of the holding part is used for fixing a rotating shaft arranged on the outer side of the shaft sleeve part.

As a preferred embodiment of the temperature metal bath with a lifting structure according to the invention, wherein: the rotary part comprises a first rotary part and a second rotary part, wherein a tooth structure is arranged on the outer side of the first rotary part and matched with the tooth structure on the outer side of the second rotary part, and the outer diameter of the second rotary part is twice that of the first rotary part.

As a preferred embodiment of the temperature metal bath with a lifting structure according to the invention, wherein: the rotating part further comprises a first driving piece connected with the second rotating piece.

As a preferred embodiment of the temperature metal bath with a lifting structure according to the invention, wherein: the reaction vessel is provided with a fixing part, and a signal detection part is arranged at the upper end of the fixing part and can detect the reaction vessel.

As a preferred embodiment of the temperature metal bath with a lifting structure according to the invention, wherein: the reaction container is provided with a temperature real-time sensor for monitoring the temperature of the reaction container in real time.

The invention also provides a method for realizing temperature difference by using the metal bath with the lifting structure, and the efficiency of nucleic acid amplification reaction is improved.

In order to solve the technical problems, the invention provides the following technical scheme: a method for achieving a temperature difference in a metal bath with a lifting structure, comprising adding a nucleic acid solution to a first solution zone of a reaction vessel; the temperature metal bath with the lifting structure drives the nucleic acid solution in the first solution zone of the reaction vessel to flow into the second solution zone through rotating centrifugation; after the nucleic acid solution is heated at the differential temperature in the first solution region and the second solution region, the signal detection part collects data to obtain the reaction result of the nucleic acid solution.

The invention has the beneficial effects that: the invention provides a lifting structure and a temperature metal bath with the same, which can realize the high-low temperature switching in the PCR nucleic acid amplification reaction process more quickly by realizing differential temperature heating, thereby effectively accelerating the reaction process and the efficiency.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the description of the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. Wherein:

FIG. 1 is a schematic view of the entire structure of a lifting structure according to a first embodiment of the present invention;

FIG. 2 is a schematic cross-sectional view showing the whole structure of the rotary shaft body according to the first embodiment of the present invention;

FIG. 3 is a schematic cross-sectional view showing the whole structure of the rotary lifting body according to the first embodiment of the present invention;

FIG. 4 is a schematic cross-sectional view showing the overall structure of a lifting structure according to a first embodiment of the present invention;

FIG. 5 is a schematic cross-sectional view showing the overall structure of the lifting structure with a holding member in the first embodiment of the present invention;

FIG. 6 is an enlarged view of the entire structure and part of the second embodiment of the present invention provided by the temperature metal bath with the elevating structure;

FIG. 7 is a schematic top view of the temperature controlling means of the second embodiment provided by the temperature metal bath with a lifting structure of the present invention;

FIG. 8 is a schematic view showing the overall structure of the reaction vessel in a second embodiment provided by the temperature metal bath with a lifting structure according to the present invention;

FIG. 9 is a schematic top view of a temperature control member of a third embodiment provided in a temperature metal bath with a lifting structure in accordance with the present invention;

FIG. 10 is a schematic view of a temperature controlling means according to a third embodiment of the present invention provided by a temperature metal bath with a lifting structure;

FIG. 11 is a schematic view showing the overall structure of the temperature controlling means in a third embodiment provided by the temperature metal bath with a lifting structure according to the present invention;

FIG. 12 is a schematic view showing the overall structure of the temperature controlling means in a third embodiment provided by the temperature metal bath with a lifting structure according to the present invention;

FIG. 13 is a schematic view showing the overall structure of a third embodiment of the present invention provided by a temperature metal bath with a lifting structure;

FIG. 14 is a schematic view showing the overall structure of the signal detecting unit in a fourth embodiment provided by the temperature metal bath with a lifting structure according to the present invention;

FIG. 15 is a schematic view showing the overall structure of a fourth embodiment of the present invention provided by a temperature metal bath with a lifting structure;

FIG. 16 is a schematic view showing the overall structure of a sixth embodiment of the present invention provided by a temperature metal bath with a lifting structure.

Detailed Description

In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to specific embodiments thereof which are illustrated in the appended drawings.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways other than those described herein, and persons skilled in the art will readily appreciate that the present invention is not limited to the specific embodiments disclosed below.

In the following detailed description of the embodiments of the present invention, reference is made to the accompanying drawings, which form a part hereof, and in which are shown by way of illustration only, and in which is shown by way of illustration only, and in which the scope of the invention is not limited for ease of illustration. In addition, the three-dimensional dimensions of length, width and depth should be included in actual fabrication.

Further still, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic may be included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.

The present invention firstly provides a lifting structure, which comprises a rotating shaft body 100 and a lifting assembly 200, wherein lifting is realized through cooperation of the rotating shaft body 100 and the lifting assembly 200, the rotating shaft body 100 comprises a first shaft body 101 and a second shaft body 102, and when the first shaft body 101 rotates in the positive direction, the second shaft body 102 is lifted for a certain distance and rotates along with the first shaft body 101. When the first shaft 101 rotates in the opposite direction, the second shaft 102 is lowered by a certain distance and rotates with the first shaft 101. As shown in fig. 1, the rotating shaft body 100 includes a first shaft body 101 and a second shaft body 102, and specifically includes: referring to fig. 2 to 5, the lifting assembly 200 includes a boss 201 and a protrusion 202. The shaft sleeve portion 201 is connected with the first shaft body 101, the shaft sleeve portion 201 is in a hollow structure, and is connected and arranged above the first shaft body 101, and an upward inclined conversion channel 201a is arranged on the shaft sleeve portion, wherein the number of the conversion channels 201a can be multiple, and the number is not limited to the number shown in the drawings. Preferably, the conversion channels 201a are arranged on two sides of the shaft sleeve part 201 in a double symmetrical manner, so that stress can be balanced, the stress is kept uniform, and the service life and the safety of the components are ensured. The protruding piece 202 is connected with the second shaft body 102, and the protruding piece 202 cooperates with the conversion channel 201a, and when the second shaft body 102 and the shaft sleeve part 201 are subjected to external forces with different directions, the protruding piece 202 moves along the track of the conversion channel 201a, so that the first shaft body 101 and the second shaft body 102 can lift. In this embodiment, the second shaft body 102 is sleeved with a one-way bearing S, and it should be noted that the meaning of the one-way bearing S is: can move freely in one direction and can be locked in the other direction, that is, the metal shell of the one-way bearing S contains a plurality of rolling shafts, rolling pins or rolling balls, and the rolling seat (hole) is shaped so that the rolling seat can roll only in one direction and can generate great resistance in the other direction.

The shaft sleeve portion 201 is connected with the first shaft body 101, the protruding member 202 is connected with the second shaft body 102, when the first shaft body 101 rotates forward, the second shaft body 102 is matched with the first shaft body 101, the protruding member 202 climbs slowly along the transition channel 201a, and after a certain climbing distance, the second shaft body 102 rotates forward along with the first shaft body 101. When the first shaft body 101 rotates reversely, the second shaft body 102 and the first shaft body 101 cooperate, the protruding member 202 descends slowly along the switching path 201a, and after a certain distance, the second shaft body 102 rotates reversely along with the first shaft body 101.

Preferably, the lifting assembly 200 further includes a fixed supporting portion 203 and a damping member 204, the fixed supporting portion 203 is disposed outside the second shaft body 102, the width of the fixed supporting portion is greater than that of the second shaft body 102, the damping member 204 is disposed at the connection portion between the fixed supporting portion 203 and the one-way bearing S, and the damping member 204 is capable of damping the one-way bearing S and the lower end of the fixed supporting portion 203. The hollow portion of the damping member 204 is circular, but not limited to this shape, and may be configured in different shapes according to the requirements of different products, for example: step-like, etc. In this embodiment, the damping member 204 is made of a material having relatively good wear resistance, such as wear-resistant rubber, and is toothed on the inside to provide friction and generate resistance, and in this embodiment, a rubber wear ring 204a is preferred.

Specifically, referring to fig. 4 and 5, the lifting structure of the present invention includes a first shaft body 101, a second shaft body 102 and a lifting assembly 200, the lifting assembly 200 includes a shaft sleeve portion 201, a protrusion 202, a fixed supporting portion 203 and a damping member 204, and the first shaft body 101, the second shaft body 102 and the lifting assembly 200 fix the overall structure by a holding member 600, the protrusion 202 ascends or descends along a transition channel 201a by matching the second shaft body 102 and the first shaft body 101, wherein a one-way bearing S is sleeved on the second shaft body 102, the damping member 204 is disposed at a "connection portion" between the fixed supporting portion 203 and the one-way bearing S, which can generate a damping effect on the lower end of the fixed supporting portion 203, and it should be noted that, here, the "connection portion" means that the fixed supporting portion 203 is separated from the damping member 204 when the protrusion 202 drives the second shaft body 102 to ascend along the transition channel 201 a. That is, when the first shaft body 101 rotates forward, the second shaft body 102 also rotates forward (when the protruding member 202 is at the low position of the conversion channel 201 a) by the protruding member 202 being engaged with the conversion channel 201a, the one-way bearing S is started, that is, the inner ring and the outer ring rotate simultaneously, so that linkage is generated, and no friction exists between the outer ring and the inner ring. Then the first shaft body 101 is reversed, the second shaft body 102 should be reversed, at this time, the one-way bearing S is not started, that is, the inner ring contacting with the second shaft body 102 rotates, and the outer ring is not moved, so the damping member 204 does not rotate, but the damping member 204 contacts with the fixed supporting portion 203, the rotation speed of the first shaft body 101 becomes larger, the damping member 204 generates resistance to the second shaft body 102, the rotation speed of the second shaft body 102 is reduced, even the second shaft body 102 does not rotate, because the rotation speed of the first shaft body 101 is unequal to the rotation speed of the second shaft body 102, a difference is generated, the power causes the protrusion 202 to move along the conversion channel 201a, the second shaft body 102 has a certain height change relative to the first shaft body 101 until the protrusion 202 moves to the uppermost end of the track of the conversion channel 201a, the damping member 204 is separated from the fixed supporting portion 203, and friction force is not generated by contact. At the same time, after the second shaft body 102 is lifted, the damping member 204 is separated from the fixed supporting portion 203, and in this state, the damping member 204 does not generate resistance to the second shaft body 102, so the first shaft body 101 and the second shaft body 102 simultaneously rotate at the same speed.

Preferably, when the boss 202 moves to the uppermost end of the track of the transition channel 201a, the damping member 204 is "just" away from the fixed support 203, i.e., the displacement by which the boss 202 climbs along the transition channel 201a is equal to the distance by which the damping member 204 contacts the fixed support 203.

A second embodiment of the present invention, referring to fig. 6 to 8, is different from the first embodiment in that: as shown in fig. 6, the temperature metal bath body with the elevating structure further includes a temperature controlling part 400 and a fixing part 500. Referring to fig. 7, in the temperature control component 400, the first temperature region 410 and the second temperature region 420 can be formed according to the set value output temperature, and the first temperature region 410 and the second temperature region 420 are located in the same plane region with equal distance from the rotation center point, that is, the first temperature region 410 and the second temperature region 420 are located at the same radius position from the rotation center point, and in this embodiment, the temperatures formed by the first temperature region 410 and the second temperature region 420 may be different or the same. The fixing member 500 fixes the target reaction vessel M at the elevation structure (i.e., the upper end of the second shaft body 102 in the first embodiment), and is capable of generating a relative distance change between the temperature controlling member 400 and the elevation change of the elevation structure, thereby differentially heating the reaction vessel M. Referring to fig. 8, the reaction vessel M includes a first solution region a and a second solution region b, a certain amount of nucleic acid solution is injected into the reaction vessel M by manually adding the liquid to the second solution region b, the instrument top flip cover is opened, the reaction vessel M and the fixing member 500 are sequentially placed in the operation region, fixed with an elliptical screw cap, and then the flip cover is covered. In this embodiment, the first temperature zone 410 and the second temperature zone 420 are disposed at positions enveloping the first solution zone a and heating the first solution zone a. Specific embodiments: the reaction vessel M is fixed on the elevation structure by the fixing member 500 in an initial state of the elevation structure (the protrusion 202 is at a low position of the switching passage 201 a), the elevation structure is moved by the forward rotation and the reverse rotation of the first shaft body 101, the temperature controlling member 400 is separated from the reaction vessel M when the elevation structure is "up", the temperature controlling member 400 is attached to the reaction vessel M when the elevation structure is "down", the reaction vessel M is in contact with the first temperature region 410 and the second temperature region 420 in the process of "up" and "down", the first temperature region 410 and the second temperature region 420 heat the first solution region a after "down", and the reaction vessel M is separated from the first temperature region 410 and the second temperature region 420 after "up", and does not heat again at this time, and the temperature is lowered. Therefore, the heating temperature is different between the "rising" and "falling", and the reaction vessel M is rotated all the time, so that an experiment of performing differential temperature heating in the reaction vessel M is realized.

Preferably, in order to increase the heating efficiency, a heat conductive aluminum block is attached to the bottom of the reaction vessel M, so that when the reaction vessel M contacts the first temperature region 410 and the second temperature region 420, the heating time is increased because the heat conductivity of the metal is good.

A third embodiment of the present invention, as shown in fig. 9 to 13, differs from the second embodiment in that: as shown in fig. 9, the temperature control component 400 further includes a third temperature area 430 and a fourth temperature area 440, which are located in the same plane area with equal distance from the rotation center point, that is, the third temperature area 130 and the fourth temperature area 140 are located in the same radius position from the rotation center point and are matched to encapsulate the second solution area b, where the shape of the third temperature area 430 and the fourth temperature area 440 matched to each other may be rectangular, may be circular, in the illustration of the present embodiment, taking the shape of the circle of the second temperature area b matched to each other as an example, but not limited to this shape, and other shapes can encapsulate the second solution area b within the protection scope of the present invention, and are not repeated. It should be noted that, the meaning of enveloping the second solution region b by the third temperature region 430 and the fourth temperature region 440 is: the added nucleic acid solution is firstly passed through the second solution zone b and then is thrown into the first solution zone a by rotating, so that if the second solution zone b is firstly preheated by the third temperature zone 430 and the fourth temperature zone 440, the preheated liquid is thrown into the first solution zone a, and then the first solution zone a is heated by the first temperature zone 410 and the second temperature zone 420, the time required by heating can be shortened, the progress is quickened, and the time cost is saved. Specifically, the main body of the device includes a rotating shaft body 100, a lifting assembly 200, a temperature control member 400, and a fixing member 500, wherein the lifting assembly 200 includes a shaft sleeve portion 201 and a protrusion 202. The shaft sleeve part 201 is connected with the first shaft body 101, the shaft sleeve part 201 is of a hollow structure, the shaft sleeve part 201 is arranged above the rotating shaft body 100, an upward inclined conversion channel 201a is arranged on the shaft sleeve part 201, the protruding piece 202 is connected with the second shaft body 102, the protruding piece 202 is matched with the conversion channel 201a, and when the second shaft body 102 and the shaft sleeve part 201 are subjected to external forces in different directions, the protruding piece 202 moves along the track of the conversion channel 201a, so that the first shaft body 101 and the second shaft body 102 can lift. The second shaft body 102 is sleeved with a one-way bearing S, and it should be noted that the meaning of adopting the one-way bearing S here is that: can move freely in one direction and can be locked in the other direction, that is, the metal shell of the one-way bearing S contains a plurality of rolling shafts, rolling pins or rolling balls, and the rolling seat (hole) is shaped so that the rolling seat can roll only in one direction and can generate great resistance in the other direction. The lifting assembly 200 further comprises a fixed supporting portion 203 and a damping member 204, the fixed supporting portion 203 is arranged outside the second shaft body 102 in contact with the second shaft body 102, the width of the fixed supporting portion is larger than that of the second shaft body 102, the damping member 204 is arranged at the joint of the fixed supporting portion 203 and the one-way bearing S, and the damping member 204 can generate damping effect on the one-way bearing S and the lower end of the fixed supporting portion 203.

The first shaft body 101 rotates at a low speed, the shaft sleeve part 201 is connected with the first shaft body 101, the protruding part 202 is connected with the second shaft body 102, when the first shaft body 101 rotates positively, the second shaft body 102 also rotates positively (at this time, the protruding part 202 is positioned at the low position of the conversion channel 201 a) through the cooperation of the protruding part 202 and the conversion channel 201a, the damping part 204 directly rubs with the fixed supporting part 203, the unidirectional bearing S is started, namely, the inner ring and the outer ring rotate simultaneously in the same direction to generate linkage, no friction exists between the outer ring and the inner ring, but friction exists between the damping part 204 and the bearing S. With the first shaft body 101 and the second shaft body 102 rotating in the same direction for a period of time at a low speed, then the first shaft body 101 is reversed, and the second shaft body 102 is reversed, because the one-way bearing S is not started, that is, the inner ring contacting with the first shaft body 101 rotates, and the outer ring is not moved, so that the damping part 204 does not rotate, and the damping part 204 contacts with the fixed supporting part 203, friction force is generated, resistance is generated on the second shaft body 102, the rotation speed of the second shaft body 102 is reduced, so that a speed difference is generated between the movement speed of the second shaft body 102 and the movement speed of the first shaft body 101, the power causes the protrusion 202 to move along the conversion channel 201a, the second shaft body 102 has a certain height change relative to the first shaft body 101 until the protrusion 202 moves to the uppermost end of the track of the conversion channel 201a, and the damping part 204 is separated from the fixed supporting part 203, so that friction force is not generated. At the same time, after the second shaft body 102 is lifted, the damping member 204 is separated from the fixed supporting portion 203, and in this state, the damping member 204 does not generate resistance to the second shaft body 102, so the first shaft body 101 and the second shaft body 102 simultaneously rotate at the same speed.

After the first shaft body 101 and the second shaft body 102 are lifted, the first shaft body 101 is rotated at a high speed and the second shaft body 102 is driven to realize temperature difference.

When the first shaft body 101 rotates forward at a low speed, the third temperature zone 430 and the fourth temperature zone 440 preheat the second solution zone b, after preheating for a period of time, the first shaft body 101 and the second shaft body 102 complete lifting and then rotate at a high speed in a reverse direction through the cooperation of the protruding piece 202 and the conversion channel 201a, so that the reaction container M is centrifuged at a high speed, and the solution in the second solution zone b is thrown into the first solution zone a. Preferably, the temperature of the third temperature zone 430 and the fourth temperature zone 440 may be 42 ℃ or 95 ℃ during preheating, and the temperatures of the two temperature zones may be different or the same. The rotation is at a high speed for a period of time and is slowly slowed down so that a "drop" function is accomplished between the boss 202 and the transition channel 201 a. When the speed is changed from high speed to low speed, the first shaft body 101 is rotated forward, the second shaft body 102 is rotated forward, the unidirectional bearing S is started, namely the inner ring and the outer ring rotate in the same direction simultaneously, linkage is generated, friction force is not generated between the outer ring and the inner ring, but friction force is generated between the damping part 204 and the bearing S, and the damping part 204 is not contacted with the fixed supporting part 203, so that the movement speed of the first shaft body 101 is slightly smaller than the movement speed of the second shaft body 102 under the driving of the damping part 204, a relative speed difference is generated, the power enables the protruding part 202 to move along the conversion channel 201a, and when the protruding part 202 moves down one end distance along the conversion channel 201a, the damping part 204 contacts with the fixed supporting part 203, friction force is generated, and resistance is also generated. Under the action of the damping member 204, the protrusion 202 moves to its lowest end along the transition channel 201a, completing the "descending" process, and simultaneously, the reaction vessel M is also driven to descend. Preferably, in order to enhance heating efficiency, a heat conductive aluminum block is attached to the bottom of the reaction vessel M, so that when the first solution zone a is in contact with the first temperature zone 410 and the second temperature zone 420, and the second solution zone b is in contact with the third temperature zone 430 and the fourth temperature zone 440, heating time is enhanced because of better heat conductivity of the metal.

The lifting structure is further provided with a holding member 600 to fix the whole structure, in this embodiment, referring to fig. 10 and 11, the temperature control member 400 further includes a first temperature control module 401, a first heating device 402 and a first sensor 403, the first temperature control module 401 and the first heating device 402 are connected, and the first temperature control module 401 controls a temperature change of the first heating device 402, and heats the reaction container M through the temperature change. After the first heating device 402 is placed in the first temperature zone 410 and the second temperature zone 420, respectively, the temperature inside is sensed by the first sensor 403, and the temperature values in the first temperature zone 410 and the second temperature zone 420 are controlled by the first temperature control module 401. Preferably, as a preferred embodiment of the present embodiment, the first temperature control module 401 further includes a heat transfer material 402-1, the first heating device 402 is disposed on the heat transfer material 402-1, the first temperature zone 410 is formed by the heat transfer material 402-1, and the reaction vessel M is heated in contact with the heat transfer material 402-1. Similarly, the principle and method of heating the reaction vessel M in the second temperature zone 420 are similar to those of the first temperature zone 410, and will not be described again. In this embodiment, the temperatures set in the first temperature region 410 and the second temperature region 420 may be the same or different.

Preferably, the first temperature zone 410 and the second temperature zone 420 are initially set at the same temperature, while the first solution zone a is heated, such as: the temperature initially set is 95 ℃, and after a period of time, the first temperature zone 410 and the second temperature zone 420 are adjusted to different temperatures, such as: the second temperature zone 420 is set to 42 c and the first temperature zone 410 is set to 95 c so that when the rotation is heated, temperature differentiation is achieved.

Preferably, because the area of the second temperature zone 420 is relatively large, a plurality of first temperature control modules 401 and first temperature sensors 403 may be placed in the second temperature zone 420 in consideration of uniform heating. Referring to fig. 12, in order to dissipate heat of the temperature controlling part 400 and prevent the temperature from being excessively high, a plurality of heat radiating fins 442 and heat radiating fans 441 are provided at the lower end of the temperature controlling part 400, and the heat is blown away by the heat radiating fans 441 and then passes through the heat radiating fins 442, so that the heat inside can be reduced, and at the same time, convenience is brought when the temperature of the part needs to be reduced. It should be noted that the number of the cooling fans 441 and the cooling fins 442 is not limited to the number shown in the drawings, and other numbers are also within the scope of the present invention, and are not described.

The temperature control part 400 further includes a second temperature control module 401', a second heating device 402', and a first sensor 403', the first temperature control module 401' and the second heating device 402' are connected, and the second temperature control module 401' controls a temperature variation of the first heating device 402', and preheats the reaction container M through the temperature variation. After the second heating device 402' is placed in the third temperature zone 430 and the fourth temperature zone 440, respectively, the temperature inside is sensed by the first sensor 403', and the temperature values in the third temperature zone 430 and the fourth temperature zone 440 are controlled by the first temperature control module 401 '. Referring to fig. 13, a rotation shaft 601 is disposed outside the shaft sleeve portion 201 of the first shaft body 101, a rotation shaft 602 is disposed on the rotation shaft body 102 of the first shaft body 101, and both the rotation shaft 601 and the rotation shaft 602 are bearings, although the rotation shaft 601 and the rotation shaft 602 are ball bearings in the drawing, they are not limited to ball bearings, but may be other bidirectional bearings, which are not listed here, and other types are within the scope of the present invention. And there is no mutual friction between the inner and outer rings. Wherein, a first rotating member 701 is disposed between the rotating shaft 601 and the rotating shaft 602, the first rotating member 701 is matched with a second rotating member 702, the second rotating member 702 is disposed on the motor 900, and the motor drives the second rotating member 702 to rotate, and then the first rotating member 701 rotates along with the second rotating member. Preferably, a servo motor can be selected for the motor in the motor driving, so that the reached rotating speed is higher, for example, if the user needs, the rotating speed can reach 6000r/min through the servo motor, so that the reaction container M is centrifuged at a high speed, and the solution in the second solution zone b is thrown into the first solution zone a. It should be noted that: the first rotating member 701 and the second rotating member 702 may be pulleys, and may be driven by gears or the like. At high rotational speeds, if the reaction vessel M is in contact with the temperature controlling member 400 for a long time, wear is increased, so that this embodiment makes the apparatus more structurally stable, and further, by changing the distance to reduce damage and switching back and forth between two independent different temperatures, the advantages of the second and third embodiments are combined, and testing is performed.

Preferably, the outer diameter of the second rotating member 702 is twice the outer diameter of the first rotating member 701.

In this embodiment, it is preferable that the first temperature zone 410 and the second temperature zone 420 are set to a temperature of 95 ℃ by the first temperature control module 401, the first heating device 402 and the first sensor 403, the third temperature zone 430 and the fourth temperature zone 440 are set to a temperature of 42 ℃ or 95 ℃ by the second temperature control module 401', the second heating device 402' and the first sensor 403', and the first temperature zone 410 and the second temperature zone 420 are set to a temperature of 42 ℃ by the first temperature control module 401, the first heating device 402 and the first sensor 403 after the solution in the second solution zone b is thrown into the first solution zone a by the high-speed centrifugation of the reaction vessel M.

When the nucleic acid amplification reaction is completed, the motor stops rotating, at this time, the second heating device 402' and the first heating device 402 stop heating, at this time, the reaction vessel M needs to be removed from the device and subjected to secondary liquid adding, after the secondary liquid adding, the reaction vessel M is fixed by the fixing component 500, the motor rotates anticlockwise, the reaction vessel M is driven to rise to a high position, the motor rotates at a high speed, the reaction vessel M is enabled to be centrifuged at a high speed, the solution in the reaction vessel M is uniformly distributed into the first solution zone a from the second solution zone b, after that, the rotation speed of the motor is reduced until the reaction vessel M stops, test strips in the reaction vessel M are observed after a period of time, and an experimental result is obtained.

A fourth embodiment of the present invention, as shown in fig. 14 and 15, is different from the third embodiment in that: the temperature metal bath of the elevating structure further includes a signal detecting member 800, as shown in fig. 15, provided at the upper end of the fixing member 500, capable of detecting the reaction vessel M. Referring to fig. 14, a light source body 801 is provided in a signal detecting part 800, light is emitted by the light source body 801, passes through a convex lens 802 and a plane mirror 803, and irradiates light downward to a position to be detected 804, reflects an optical signal to an optical microscope 805 through the position to be detected 804, and passes information through a processor to obtain a detection result.

Preferably, a temperature real-time sensor is arranged on the reaction container M to monitor the temperature of the reaction container M in real time.

In the present embodiment, the meaning of adding the signal detecting section 800 is: the second liquid adding is performed without taking down the reaction container M after the first liquid adding, and the data can be directly collected by the signal detecting unit 800 to obtain the detection result.

Specifically, the main body of the device in this embodiment includes a rotating shaft body 100, a lifting assembly 200, a temperature control component 400, a fixing component 500, a holding component 600 and a signal detection component 800, where the lifting assembly 200 includes a shaft sleeve 201, a protruding member 202, a fixed supporting portion 203 and a damping component 204, the protruding member 202 ascends or descends along the shaft sleeve 201 through the cooperation of the second shaft body 102 and the first shaft body 101, where the second shaft body 102 is sleeved with a unidirectional bearing S, the damping component 204 is disposed at a "connection portion" between the fixed supporting portion 203 and the unidirectional bearing S, and can generate a damping effect on the lower end of the fixed supporting portion 203, and it should be noted that, here, the "connection portion" means that when the protruding member 202 ascends along the transition channel 201a to drive the second shaft body 102, the fixed supporting portion 203 leaves the damping component 204.

When the first shaft body 101 rotates positively, the second shaft body 102 also rotates positively (when the protruding member 202 is at the low position of the conversion channel 201 a) by the cooperation of the protruding member 202 and the conversion channel 201a, the one-way bearing S is started, that is, the inner ring and the outer ring rotate simultaneously to generate linkage, and no friction exists between the outer ring and the inner ring. Then the first shaft body 101 is reversed, the second shaft body 102 should be reversed, at this time, the one-way bearing S is not started, that is, the inner ring contacting the second shaft body 102 rotates, and the outer ring is not moved, so the damping member 204 does not rotate, but the damping member 204 contacts the fixed supporting portion 203, the rotation speed of the first shaft body 101 becomes larger, the damping member 204 generates resistance to the second shaft body 102, the rotation speed of the second shaft body 102 is reduced, even the second shaft body 102 is not rotated, the rotation speed of the first shaft body 101 and the rotation speed of the second shaft body 102 are different, the difference is generated, the power causes the protrusion 202 to move along the conversion channel 201a, the second shaft body 102 has a certain height change relative to the first shaft body 101 until the protrusion 202 moves to the uppermost end of the track of the conversion channel 201a, and the protrusion 202 and the conversion channel 201a stop moving relatively. At the same time, after the second shaft body 102 is lifted, the damping member 204 is separated from the fixed supporting portion 203, and in this state, the damping member 204 does not generate resistance to the second shaft body 102, so the first shaft body 101 and the second shaft body 102 simultaneously rotate at the same speed.

The temperature controlling part 400 includes a first temperature zone 410, a second temperature zone 420, a third temperature zone 430 and a fourth temperature zone 440, and when the first shaft body 101 rotates forward at a low speed, the third temperature zone 430 and the fourth temperature zone 440 preheat the second solution zone b, and the first temperature zone 410 and the second temperature zone 420 heat the first solution zone a. After preheating for a period of time, through the cooperation of the protruding piece 202 and the conversion channel 201a, the first shaft body 101 and the second shaft body 102 complete lifting and then reversely rotate at a high speed, so that the reaction container M is centrifuged at a high speed, and the solution in the second solution zone b is thrown into the first solution zone a. Preferably, the temperature of the third temperature zone 430 and the fourth temperature zone 440 may be 42 ℃ or 95 ℃ during preheating, and the temperatures of the two temperature zones may be different or the same. The rotation is at a high speed for a period of time and is slowly slowed down so that a "drop" function is accomplished between the boss 202 and the transition channel 201 a. When the speed is changed from high speed to low speed, the first shaft body 101 is rotated forward, the second shaft body 102 is rotated forward, the unidirectional bearing S is started, namely the inner ring and the outer ring rotate in the same direction simultaneously, linkage is generated, friction force is not generated between the outer ring and the inner ring, but friction force is generated between the damping part 204 and the bearing S, and the damping part 204 is not contacted with the fixed supporting part 203, so that the movement speed of the first shaft body 101 is slightly smaller than the movement speed of the second shaft body 102 under the driving of the damping part 204, a relative speed difference is generated, the power enables the protruding part 202 to move along the conversion channel 201a, and when the protruding part 202 moves down one end distance along the conversion channel 201a, the damping part 204 contacts with the fixed supporting part 203, friction force is generated, and resistance is also generated. Under the action of the damping member 204, the protrusion 202 moves to its lowest end along the transition channel 201a, completing the "descending" process, and simultaneously, the reaction vessel M is also driven to descend.

In this embodiment, the temperature control component 400 further includes a first temperature control module 401, a first heating device 402, and a first sensor 403, where the first temperature control module 401 and the first heating device 402 are connected, and the first temperature control module 401 controls a temperature change of the first heating device 402, and heats the reaction container M through the temperature change. After the first heating device 402 is placed in the first temperature zone 410 and the second temperature zone 420, respectively, the temperature inside is sensed by the first sensor 403, and the temperature values in the first temperature zone 410 and the second temperature zone 420 are controlled by the first temperature control module 401. Since the temperatures set in the first temperature zone 410 and the second temperature zone 420 are the same in this embodiment, the same type or model of the first temperature control module 401 and the first heating device 402 may be used.

The temperature control part 400 further includes a second temperature control module 401', a second heating device 402', and a first sensor 403', the first temperature control module 401' and the second heating device 402' are connected, and the second temperature control module 401' controls a temperature variation of the first heating device 402', and preheats the reaction container M through the temperature variation. After the second heating device 402' is placed in the third temperature zone 430 and the fourth temperature zone 440, respectively, the temperature inside is sensed by the first sensor 403', and the temperature values in the third temperature zone 430 and the fourth temperature zone 440 are controlled by the second temperature control module 401 '. Since the temperatures set in the third temperature zone 430 and the fourth temperature zone 440 are the same in this embodiment, the same type or model of the second temperature control module 401 'and the second heating device 402' may be used.

The outer side of the shaft sleeve portion 201 of the first shaft body 101 is provided with a rotating shaft 601, the rotating shaft 200 of the rotating shaft body 100 is provided with a rotating shaft 602, the rotating shaft 601 and the rotating shaft 602 are bearings, and the rotating shaft 601 and the rotating shaft 602 are ball bearings, but not limited to the ball bearings, but can be other bidirectional bearings, which are not listed here, and other types are all within the scope of the present invention. And there is no mutual friction between the inner and outer rings. Wherein, a first rotating member 701 is disposed between the rotating shaft 601 and the rotating shaft 602, the first rotating member 701 is matched with a second rotating member 702, the second rotating member 702 is disposed on the motor 900, and the motor drives the second rotating member 702 to rotate, and then the first rotating member 701 rotates along with the second rotating member. Preferably, a servo motor can be selected for the motor in the motor driving, so that the reached rotating speed is higher, for example, if the user needs, the rotating speed can reach 6000r/min through the servo motor, so that the reaction container M is centrifuged at a high speed, and the solution in the second solution zone b is thrown into the first solution zone a. It should be noted that: the first rotating member 701 and the second rotating member 702 may be pulleys, and may be driven by gears or the like. At high rotational speeds, if the reaction vessel M is in contact with the temperature controlling member 400 for a long time, wear is increased, so that this embodiment makes the apparatus more structurally stable, and further, by changing the distance to reduce damage and switching back and forth between two independent different temperatures, the advantages of the second and third embodiments are combined, and testing is performed.

In the present embodiment, the first temperature zone 410 and the second temperature zone 420 set the temperature to a high temperature through the first temperature control module 401, the first heating device 402, and the first sensor 403, such as: 95 ℃, the third temperature zone 430 and the fourth temperature zone 440 set the temperature to another temperature by the second temperature control module 401', the second heating device 402' and the first sensor 403', such as: after the solution in the second solution zone b is thrown to the first solution zone a by high-speed centrifugation of the reaction vessel M at 42 c or 95 c, the first temperature zone 410 and the second temperature zone 420 are set to a temperature of 42 c by the first temperature control module 401, the first heating device 402 and the first sensor 403.

When the nucleic acid amplification reaction is completed, the motor stops rotating, and at this time, the second heating device 402' and the first heating device 402 stop heating, and data is collected by the signal detection part 800, thereby obtaining a detection result.

Regarding the heating temperature control mode of the present invention, there is also provided a fifth embodiment of the temperature metal bath for differential temperature heating of the present invention, wherein the temperature control unit 400 includes an electromagnetic induction device 402a and an electromagnetic heating sheet, the first temperature control module 101 is connected to and controls the electromagnetic induction device to generate an alternating magnetic field, the electromagnetic induction device performs temperature control output on the electromagnetic heating sheet containing metal material through the alternating magnetic field, the electromagnetic heating sheet is attached to the reaction vessel M, and differential temperature heating is performed on the reaction vessel M through temperature control, that is, the alternating magnetic field generating device and the electromagnetic heating sheet perform differential temperature heating on the reaction vessel M through an electromagnetic heating principle.

In the first five embodiments, the damping effect is generated on the unidirectional bearing S and the lower end of the fixed support portion 203 by the damping member 204, but when the damping member 204 adopts the rubber wear ring 204a, it is difficult to ensure the processing precision, and after the assembly is completed, there are problems that the resistance applied to the main rotation shaft is difficult to adjust, etc., therefore, referring to fig. 16, the temperature metal bath for differential temperature heating according to the present invention provides the sixth embodiment, in this embodiment, the damping member 204 adopts the stopper 204b instead of the rubber wear ring 204a, and preferably, the stopper 204b adopts a ball plunger with adjustable resistance.

The plug 204b is divided into a first ball plunger 204b-1 and a second ball plunger 204b-2, wherein the first ball plunger 204b-1 is abutted against the outer side of the one-way bearing S, friction is generated when the first ball plunger 204b-1 contacts the one-way bearing S, and friction force is generated when the second ball plunger 204b-2 contacts the fixed supporting portion 203.

Preferably, the first ball plunger 204b-1 and the second ball plunger 204b-2 are both centered on the rotational axis 100

The array center is respectively arrayed into a plane, and a plurality of (i.e. two or more) first ball plunger 204b-1 or second ball plunger 204b-2 are arranged on the plane where the first ball plunger 204b-1 or the second ball plunger 204b-2 is located. The planes of the first ball plunger 204b-1 and the second ball plunger 204b-2 are parallel to each other, and the plane of the first ball plunger 204b-1 is perpendicular to the one-way bearing S. The second ball plunger 204b-2 is disposed above the first ball plunger 204 b-1.

In the present embodiment, the holding member 600 is provided with a first plunger screw hole 602-1 and a second plunger screw hole 602-2, wherein the first ball plunger 204b-1 is screwed in through the first plunger screw hole 602-1, and the second ball plunger 204b-2 is screwed in through the second plunger screw hole 602-2.

The first shaft body 101 rotates at a low speed, the shaft sleeve part 201 is connected with the first shaft body 101, the protruding part 202 is connected with the second shaft body 102, when the first shaft body 101 rotates positively, the second shaft body 102 also rotates positively (at the moment, the protruding part 202 is positioned at the low position of the conversion channel 201 a) through the cooperation of the protruding part 202 and the conversion channel 201a, the second ball plunger 204b-2 directly rubs with the fixed supporting part 203, the one-way bearing S is started, namely, the inner ring and the outer ring simultaneously rotate in the same direction to generate linkage, no friction force exists between the outer ring and the inner ring, but friction force exists between the first ball plunger 204b-1 and the bearing S. With the first shaft 101 and the second shaft 102 rotating in the same direction for a period of time at a low speed, the first shaft 101 is then reversed, and the second shaft 102 is also reversed, because the one-way bearing S is not started, that is, the inner ring contacting with the first shaft 101 rotates, and the outer ring is not moved, so the first ball plunger 204b-1 does not generate resistance, the second ball plunger 204b-2 contacts with the fixed supporting part 203, friction force is generated, the second shaft 102 generates resistance, the rotation speed of the second shaft 102 is reduced, so that the movement speed of the second shaft 102 and the movement speed of the first shaft 101 generate a speed difference, the power causes the protrusion 202 to move along the conversion channel 201a, the second shaft 102 has a certain height change relative to the first shaft 101 until the protrusion 202 moves to the uppermost end of the track of the conversion channel 201a, the second ball plunger 204b-2 is separated from the fixed supporting part 203, and friction force is not generated by contact. Meanwhile, after the second shaft body 102 is lifted, the second ball plunger 204b-2 is separated from the fixed supporting portion 203, and in this state, the second ball plunger 204b-2 does not generate resistance to the second shaft body 102, so the first shaft body 101 and the second shaft body 102 simultaneously rotate at the same speed.

The rotation is at a high speed for a period of time and is slowly slowed down so that a "drop" function is accomplished between the boss 202 and the transition channel 201 a. When the speed is changed from high speed to low speed, the first shaft body 101 is rotated forward, the second shaft body 102 is rotated forward, the one-way bearing S is started, namely the inner ring and the outer ring rotate in the same direction simultaneously, linkage is generated, friction force is generated between the outer ring and the inner ring, but friction force is generated between the first ball plunger 204b-1 and the bearing S, and the second ball plunger 204b-2 is not contacted with the fixed supporting part 203 at the moment, so that the movement speed of the first shaft body 101 is slightly smaller than the movement speed of the second shaft body 102 under the drive of the first ball plunger 204b-1, a relative speed difference is generated, the power enables the protruding piece 202 to move along the conversion channel 201a, and when the protruding piece 202 moves down one end distance along the conversion channel 201a, friction force is generated between the second ball plunger 204b-2 and the fixed supporting part 203, and resistance is also generated. Under the action of the damping member 204, the protrusion 202 moves to its lowest end along the transition channel 201a, completing the "descending" process, and simultaneously, the reaction vessel M is also driven to descend.

The invention also provides a method for realizing temperature difference of the metal bath with the lifting structure, which mainly comprises the following four steps:

First, a nucleic acid solution is added to the second solution region b of the reaction vessel M;

secondly, rotating a temperature metal bath with a lifting structure to drive a reaction container M, and throwing the solution in the second solution zone b into the first solution zone a;

secondly, starting a nucleic acid amplification reaction, and stopping heating after the reaction is completed;

finally, the signal detection part 800 is used for collecting data to obtain the result of the nucleic acid solution reaction, and the detection part 800 is used for collecting a diagnostic test strip or fluorescent detection.

Wherein the second solution zone b and the first solution zone a are both in the reaction vessel M, and the reaction vessel M is fixed in the metal bath apparatus by the fixing member 500.

The "rotation" in the second step is divided into a low-speed forward rotation, in which the temperature controlling member 400 is brought into contact with the reaction container M by the elevating structure, and a second solution zone b is preheated by the third and fourth temperature zones 430 and 440 by the second temperature controlling module 401', the first heating device 402', and the first sensor 403', the temperature being set to 42 ℃ or 95 ℃ (the temperature value being exemplified only), and the first and second temperature zones 410 and 420 by the first temperature controlling module 401, the first heating device 402, and the first sensor 403, the temperature being set to 95 ℃ (the temperature value being exemplified only) by the first solution zone a. When the rotation is reversed at a high speed, the rotation shaft body 100 and the second shaft body 102 are engaged with each other and the distance therebetween increases, that is, the two relatively rise, so that the temperature control member 400 is separated from the reaction vessel M, and at this time, the reaction vessel M is centrifuged at a high speed to throw the solution in the second solution region b into the first solution region a, thereby starting the nucleic acid amplification reaction.

When the amplification reaction is started, heating is stopped, the rotation quick freezing of the motor is reduced until the rotation is stopped, data is collected through the signal detection part 800, and a detection result is obtained, wherein the collection mode of the detection part 800 is a diagnosis test strip or fluorescence detection. It is to be understood that this application is not limited to the details or methodology set forth in the following description or illustrated in the drawings. Also, it is to be understood that the phraseology and terminology employed herein is for the purpose of description and should not be regarded as limiting.

It is important to note that the construction and arrangement of the application as shown in the various exemplary embodiments is illustrative only. Although only a few embodiments have been described in detail in this disclosure, those skilled in the art who review this disclosure will readily appreciate that many modifications are possible (e.g., variations in sizes, dimensions, structures, shapes and proportions of the various elements, values of parameters (e.g., temperature, pressure, etc.), mounting arrangements, use of materials, colors, orientations, etc.) without materially departing from the novel teachings and advantages of the subject matter described in this application. For example, elements shown as integrally formed may be constructed of multiple parts or elements, the position of elements may be reversed or otherwise varied, and the nature or number of discrete elements or positions may be altered or varied. Accordingly, all such modifications are intended to be included within the scope of present application. The order or sequence of any process or method steps may be varied or re-sequenced according to alternative embodiments. In the claims, any means-plus-function clause is intended to cover the structures described herein as performing the recited function and not only structural equivalents but also equivalent structures. Other substitutions, modifications, changes and omissions may be made in the design, operating conditions and arrangement of the exemplary embodiments without departing from the scope of the present applications. Therefore, the application is not limited to the specific embodiments, but extends to various modifications that nevertheless fall within the scope of the appended claims.

Furthermore, in an effort to provide a concise description of the exemplary embodiments, all features of an actual implementation may not be described (i.e., those not associated with the best mode presently contemplated for carrying out the invention, or those not associated with practicing the invention). It should be appreciated that in the development of any such actual implementation, as in any engineering or design project, numerous implementation-specific decisions may be made. Such a development effort might be complex and time consuming, but would nevertheless be a routine undertaking of design, fabrication, and manufacture for those of ordinary skill having the benefit of this disclosure.

It should be noted that the above embodiments are only for illustrating the technical solution of the present invention and not for limiting the same, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solution of the present invention may be modified or substituted without departing from the spirit and scope of the technical solution of the present invention, which is intended to be covered in the scope of the claims of the present invention.

Claims

1. A lifting structure, characterized in that: comprising the steps of (a) a step of,

The lifting device comprises a rotating shaft body (100) and a lifting assembly (200), wherein the rotating shaft body (100) is matched with the lifting assembly (200) to realize lifting of the rotating shaft body (100);

when the rotating shaft body (100) rotates in one direction, the rotating shaft body (100) is lifted by a certain distance; when the rotating shaft body (100) rotates in the opposite direction, the rotating shaft body (100) is lowered by a certain distance;

the rotary shaft body (100) comprises a first shaft body (101) and a second shaft body (102), and the first shaft body (101) and the second shaft body (102) are matched up and down; the lifting assembly (200) comprises a shaft sleeve part (201) and a protruding piece (202),

the shaft sleeve part (201) is of a hollow structure, is arranged at the upper end of the first shaft body (101), the second shaft body (102) rotates in the shaft sleeve part (201), and a conversion channel (201 a) inclining upwards is arranged on the shaft sleeve part (201);

the protruding piece (202) is arranged on the second shaft body (102) in a protruding shape and is matched with the conversion channel (201 a);

a temperature control unit (400) capable of forming a first temperature range in which a temperature is outputted according to a set value;

and a fixing member (500) which is provided on the lifting structure, fixes the target reaction vessel (M), and can generate relative distance change between the temperature control member (400) and the lifting change of the lifting structure, thereby performing differential temperature heating on the reaction vessel (M).

2. The lifting structure of claim 1, wherein: the lifting assembly (200) further comprises,

the fixed support part (203), the fixed support part (203) is arranged on the second shaft body (102) and has a diameter larger than that of the second shaft body (102), and a one-way bearing (S) is arranged at the lower end of the fixed support part (203);

and a damping member (204) provided at the joint between the fixed support (203) and the one-way bearing (S), wherein the damping member (204) is capable of damping the lower ends of the one-way bearing (S) and the fixed support (203).

3. The lifting structure of claim 2, wherein: the damping part (204) adopts a rubber wear-resistant ring (204 a).

4. The lifting structure of claim 2, wherein: the damping component (204) adopts a plug (204 b).

5. The lifting structure of claim 2, wherein: the maximum width of the fixed support part (203) is the same as the width of the one-way bearing (S), so that the fixed support part (203) and the one-way bearing (S) can be contacted with the damping component (204).

6. A temperature metal bath with a lifting structure, characterized in that: comprising the steps of (a) a step of,

a lifting structure as claimed in any one of claims 2 to 5;

7. The temperature metal bath with lifting structure of claim 6, wherein: the temperature control component (400) comprises,

a first temperature control module (401) for controlling the temperature change of the first heating device (402); the method comprises the steps of,

and a first heating device (402) connected to the first temperature control module (401) for differentially heating the reaction vessel (M) by temperature change.

8. The temperature metal bath with lifting structure of claim 7, wherein: the temperature control component (400) further comprises a heat transfer material (402-1), the heat transfer material (402-1) is arranged on the first heating device (402), a first temperature zone is formed by the heat transfer material (402-1), and the reaction container (M) is contacted with the heat transfer material (402-1) to be heated.

9. The temperature metal bath with lifting structure of claim 6, wherein: and further comprises a first solution zone (a) and a second solution zone (b) provided on the reaction vessel (M), the first solution zone (a) being caused to pass through the first temperature zone by rotation.

10. The temperature metal bath with lifting structure of claim 6, wherein: and a second temperature zone, wherein the first solution zone (a) of the reaction vessel (M) passes through the first temperature zone and the second temperature zone in sequence by rotating.

11. The temperature metal bath with lifting structure of claim 6, wherein: and a second temperature zone through which the first solution zone (a) of the reaction vessel (M) passes by rotation, respectively.

12. The temperature metal bath with lifting structure of claim 10, wherein: the temperature control part (400) further includes a third temperature zone and a fourth temperature zone through which the second solution zone (b) of the reaction vessel (M) passes by rotation, respectively.

13. The temperature metal bath with lifting structure according to claim 10 or 12, characterized in that: the temperature control component (400) comprises,

A second temperature control module (401 ') for controlling the second heating device (402') to output a stable temperature; the method comprises the steps of,

and a second heating device (402 ') which is connected with the second temperature control module (401') and can be close to or far away from the reaction container (M), and differential temperature heating is carried out on the reaction container (M) through distance change.

14. The temperature metal bath with lifting structure of claim 6, wherein: the lifting structure further comprises a lifting device which comprises a lifting device body,

the holding member (600) has a hollow structure, the upper end of which fixes the damping member (204), and the lower end of which fixes a rotating shaft (601) provided outside the boss portion (201).

15. The temperature metal bath with lifting structure of claim 14, wherein: also included is a method of manufacturing a semiconductor device,

the rotating part comprises a first rotating part (701) and a second rotating part (702), wherein a meshing structure is arranged on the outer side of the first rotating part (701) and matched with the meshing structure on the outer side of the second rotating part (702), and the outer diameter of the second rotating part (702) is twice that of the first rotating part (701).

16. The temperature metal bath with lifting structure of claim 15, wherein: the rotating member further includes a first driving member coupled to the second rotating member (702).

17. A temperature metal bath with lifting structure according to any one of claims 10 or 12, characterized in that: also included is a method of manufacturing a semiconductor device,

and a signal detection member (800) which is provided at the upper end of the fixing member (500) and can optically detect the reaction container (M).

18. A temperature metal bath with lifting structure according to any one of claims 10 or 12, characterized in that: the reaction vessel (M) is provided with a temperature real-time sensor, and the temperature of the reaction vessel (M) is monitored in real time.

19. A method for realizing temperature difference of a metal bath with a lifting structure, which is characterized by comprising the following steps: a temperature metal bath with a lifting structure as claimed in any one of claims 9 to 12; the method comprises the steps of,

adding the nucleic acid solution to the second solution zone (b) of the reaction vessel (M);

the temperature metal bath with the elevating structure drives the nucleic acid solution of the second solution zone (b) of the reaction vessel (M) to flow into the first solution zone (a) by rotating centrifugation;

after the nucleic acid solution is heated in the first solution region (a) and the second solution region (b) by different temperatures, the signal detection unit (800) acquires data to obtain the reaction result of the nucleic acid solution.