CN107349464A - A kind of preparation method of new medical hemostasis gel dressing - Google Patents
A kind of preparation method of new medical hemostasis gel dressing Download PDFInfo
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- CN107349464A CN107349464A CN201710503854.5A CN201710503854A CN107349464A CN 107349464 A CN107349464 A CN 107349464A CN 201710503854 A CN201710503854 A CN 201710503854A CN 107349464 A CN107349464 A CN 107349464A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0028—Polypeptides; Proteins; Degradation products thereof
- A61L26/0033—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0031—Hydrogels or hydrocolloids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0036—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/10—Polypeptides; Proteins
- A61L24/102—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/008—Hydrogels or hydrocolloids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/0085—Porous materials, e.g. foams or sponges
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/24—Crosslinking, e.g. vulcanising, of macromolecules
- C08J3/243—Two or more independent types of crosslinking for one or more polymers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2389/00—Characterised by the use of proteins; Derivatives thereof
Abstract
The present invention relates to a kind of preparation method of medical hemostatic gel dressing, by human-like collagen（LHC）It is dissolved in ultra-pure water and is made into protein solution, add inorganic salts and multiple crosslinking agent beta diimine Zn complex and 1,2,7,8 diepoxyoctanes, regulation solution ph to 2 5.5, mixed solution is put in 40 80 DEG C of water-baths after stirring and keeps 0.5 5h, again successively after high temperature steam treatment twice and washing, by drying process and the radiation sterilizations of Co 60, you can obtain a kind of novel asepsis medical hemostatic gel dressing.Medical hemostatic gel dressing good air permeability prepared by the present invention and there is anti-microbial property, wound infection will not be caused, can substantially shorten bleeding stopping period, hemostatic material after completing to stop blooding will not adhesion wound, this significantly reduces the pain of patient.
Description
Technical field
The present invention relates to a kind of preparation method of medical hemostatic gel dressing, belong to field of medical materials.
Background technology
Body is caused various epidermises or tissue injury occur and go out because of factors such as war, traffic accident, motion or diseases
Blood.In recent years many researchs show, the patient for clinically dying from excessive blood loss accounts for very big ratio, therefore prepare a kind of fast short stopping of energy
Blood and the not hemostatic material with tissue adhesion, gained time for the rescue and treatment in later stage, be one and urgently to be resolved hurrily ask
Topic.
Key property currently used for the preferable dressing of wound is generally：Nontoxic, insensitive, not allergy；Pasted with wound
Close closely, and there is good moisture-absorption characteristics, wound and dressing composition surface humid environment can be maintained；Do not require often to carry out more
Change；Microorganism can not pass through；It is cost-effective；Good mechanical protection ability is provided and is not easy to adhere to wound.Hydrogel is
A kind of high molecular polymer with tridimensional network rich in moisture, it is widely used in the neck such as organizational project and medicament slow release
Domain.Compared to other materials, hydrogel has the advantages that stickiness is good, anti-infective as hemostatic material.However, conventional hydrogels
Make the shortcomings of its haemostatic effect is poor, gas permeability is bad due to lacking pore passage structure.
Human-like collagen（LHC）It is the people source collagen type by fermenting and producing.It has good cell adhesion
Property, promote that neoblast is formed, processing characteristics is good, virus-free hidden danger, good water solubility（Avoid the cell toxicant that soda acid dissolvent residual is brought
Property）, the characteristic such as rejection is low a kind of good Implantable Medical Device biomaterial.
The content of the invention
The present invention is directed to the defects of poor air permeability of in the market hemostatic material, easy adhesion wound, and having prepared one kind can be fast
Short stopping blood and will not be with the new medical hemostasis gel dressing of tissue adhesion, this method technique is simple, the material biofacies of preparation
Capacitive is good, is expected to be widely used for wartime first-aid dressing, clinical operation hemostasis etc..
To achieve these goals, the technical solution adopted by the present invention is：
A kind of preparation method of new medical hemostasis gel dressing：By the protein-based human collagen of water-soluble macromolecule（LHC）
Water is dissolved in, adds mass ratio as 1:3-3:1 multiple crosslinking agent beta-diimine Zn complex and 1,2,7,8- diepoxyoctanes
The aqueous solution and inorganic salt solution be well mixed, and it is 2-5.5 to adjust solution ph, is placed in water bath and is crosslinked
React to obtain saliferous hydrogel；High steam processs and distilled water immersion washing are carried out to the saliferous hydrogel after crosslinking, removes nothing
Machine salt and residual monomer crosslinked dose, and be dried with Co-60 sterilization treatments, that is, obtain a kind of new medical hemostasis gel and apply
Material.
The concentration of above-mentioned human-like collagen solution is 50-400 mg/mL.
Described inorganic salts are selected from sodium chloride, sodium phosphate, dibastic sodium phosphate, ammonium sulfate, ammonium nitrate, potassium nitrate, potassium sulfate, chlorine
Change potassium, inorganic salt solution concentration is 10-350 mg/mL, and inorganic salt solution adds volume and the ratio of protein solution volume is
1:2-1:20。
The mass percent of crosslinking agent beta-diimine Zn complex solution and 1,2,7,8- the diepoxyoctane solution is dense
Degree is 0.1-5.0%, preferably 0.5-2.0%, and volume is the 1-20% of protein solution volume, preferably 5-10%.
It is 2-5.5 that above-mentioned reaction solution acid-base value regulates and controls its pH value with hydrochloric acid and sodium hydroxide solution；The temperature of cross-linking reaction
Can be 40-80 DEG C, retention time 0.5-5h, preferably 1-3 h.
In above-mentioned high steam processs twice and distilled water immersion washing, sample is kept at 110-121 DEG C for the first time
Time 5-30min, preferably retention time 10-20min, then ultra-pure water washing by soaking 2-5 days；Second by sample in 110-
121 DEG C keep 1-3 h, preferably retention time 1.5-2.5h, then ultra-pure water washing by soaking 1-3 days, control the residual of crosslinking agent
Total amount is less than 2 μ g/g.
The drying means of the new medical hemostasis gel dressing can be vacuum freeze-drying method or supercritical carbon dioxide
Seasoning.
Above-mentioned crosslinking agent beta-diimine Zn complex is according to document（Catalytic Reactions Involving C1
Feedstocks: New High-Activity Zn(II)-Based Catalysts for the Alternating
Copolymerization of Carbon Dioxide and Epoxides, J. Am. Chem. Soc. 1998, 120,
11018-11019.）Method synthesis obtain, its molecular formula is as shown in Figure 1.
The formation mechenism of medical hemostatic porous gel dressing of the present invention：Protein molecule contains abundant carboxyl and amino,
Two kinds of good functional groups are provided for intermolecular cross-linking.The unoccupied orbital of beta-diimine Zn complex intramolecular chelated zinc can be with egg
Amino in white molecule forms coordinate bond, realizes the intermolecular cross-linking of protein molecule.Beta-diimine Zn complex is to alkylene oxide
Base has asymmetric open loop catalysis, can accelerate the epoxy alkyl open loop of molecule both ends and and the egg of 1,2,7,8- diepoxyoctanes
Carboxyl in white molecule forms covalent bond, realizes the intermolecular cross-linking of protein molecule.Therefore pass through beta-diimine Zn complex pair
The open loop catalysis of 1,2,7,8- diepoxyoctane, and two kinds of crosslinking agents join work to the double cross between the intermolecular different groups of protein
With effectively realizing and enhance the intermolecular crosslinking of water-solubility protein.The addition of inorganic salts, effectively destroys water-solubility protein
The outer hydration shell of the molecule of matter, promotes the cross-linking reaction between crosslinking agent and protein molecule, appropriate inorganic salts are also simultaneous in addition plays the part of
Pore former effect has been drilled, has further optimized the pore-forming effect of hydrogel.
The present invention is that primary raw material is prepared for one by the collaboration co-crosslinking effect of double crosslinker using human-like collagen
The new Superporous hydrogels of kind.The hydrogel material has super loose structure, and porosity is high, connectivity is good and aperture size is closed
Reason, the moisture in blood can be quickly absorbed without sucking cell, so as to promote hemagglutination to realize quick-acting haemostatic powder.The water-setting
Glue material has good biocompatibility and good duct connectivity, has ensured wound gas permeability, has helped lend some impetus to wound
Healing.Compared to traditional gauze or styptic sponge, roughness is smaller, is not easy adhesion on the hydrogel material surface；Duct is good
Connectivity to be administered it is very convenient；And the less aperture of material can also prevent wound infection.It is new prepared by the present invention
Type medical hemostatic gel dressing is expected to be used for the fields such as wartime first-aid dressing, clinical operation hemostasis.
The present invention has further the advantage that：（1）Hemostatic material prepared by the present invention can substantially shorten bleeding stopping period, and this is to wound
Wound or post-operative recovery are most important；（2）Permeability prepared by the present invention is good and has anti-microbial property, will not cause wound
Infection；（3）In addition, the hemostatic material for preparing of the present invention after completing to stop blooding will not adhesion wound, this significantly reduces patient
Pain；（4）Material prepared by the present invention has loose structure so that administration tonic is very convenient.
Brief description of the drawings
Fig. 1 is the molecular structure of the beta-diimine Zn complex of synthesis；
Fig. 2 is the outside drawing of the medical hemostatic porous gel dressing sample prepared by embodiment 1；
Fig. 3 is the SEM figures of the medical hemostatic porous gel dressing sample prepared by embodiment 1；
Fig. 4 is that the gas permeability of the medical hemostatic porous gel dressing prepared by embodiment 1 investigates figure；
Medical hemostatic porous gel dressing of the Fig. 5 prepared by embodiment 1 is to rabbit arteria auricularis and liver haemostatic effect figure；
Fig. 6 is haemostatic effect figure of the styptic sponge to rabbit liver of two kinds of commercialization brands used in embodiment 1.
Embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified；Material used, reagent
Deng unless otherwise specified, commercially obtaining.
The preparation of the medical hemostatic porous gel dressing of embodiment 1
Step 1：Water-soluble human-like collagen HLC is dissolved in 10 mL distilled water and obtains the solution that concentration is 100 mg/mL,
The crosslinking agent beta-diimine Zn complex and 1 that concentration is 1% is added, each 1 mL of 2,7,8- diepoxyoctanes, adds 10mg/
ML KCl 2mL, it is well mixed, and is 4.5 with watery hydrochloric acid and sodium hydrate regulator solution pH；
Step 2：Mixed solution in step 1 is sub-packed in mould, high steam is transferred to after keeping 2h under 50 DEG C of environment
20min is kept to obtain gel first product in 121 DEG C in autoclave；
Step 3：First product gel in step 2 is washed 5 days with distilled water immersion, and once washing water is changed per 6h, remove salinity and
Residual cross-linker；First product gel after washing is transferred in high-pressure steam sterilizing pan and continues 121 DEG C of 2 h of holding, then again
Washed 2 days with distilled water immersion, control the residual total amount of crosslinking agent to be less than 2 μ g/g, obtain porous wet gel sample.
Step 4：Wet gel in rapid three is subjected to vacuum freeze drying after -80 DEG C of h of pre-freeze 3 and carries out Co-60 spokes
According to after sterilizing i.e. obtain sterile medical hemostatic porous gel dressing finished product.
Physico-chemical property sign is carried out to the medical hemostatic porous gel dressing prepared in this example, Fig. 2 is that embodiment 1 is made
Standby hemostasis gel dressing freezes the outward appearance photo of sample, it can be seen that it is in opaque milky in appearance, surface texturisation compared with
To be smooth, in loose structure；Fig. 3 is the ESEM of the porous aquagel bleeding-stopping dressing prepared（SEM）Figure.SEM figures are shown only
In being loose structure inside blood dressing, hole wall, which is that spheric granules is inter-adhesive, to be formed, and aperture is typically arrived several micro- at tens nanometers
Rice, pore structure is more uniform and densification penetrates.
The medical hemostatic porous gel dressing block of preparation is placed in filter fixed on syringe, into syringe
Carry out extruding syringe progress air filtration after being filled 5 mL air, as a result find almost there is no resistance during this, inject
The push rod of device can easily shift bottom onto.Syringe extrudes the air filtration experiment shows gas permeability of bleeding-stopping dressing, such as Fig. 4 institutes
Show, illustrate that the pore structure inside hemostatic material is mutually communicated.The material has good gas permeability and anti-adhesive properties, if
Antibacterials directly can directly be added by material and be slowly discharged into wound, therefore can will not be because of during hemostasis
Gas permeability causes wound infection, and same wound will not also fester because of long-time immersion.
The medical hemostatic porous gel dressing elasticity prepared in this example is good, and resistance to compression is strong（Maximum compression strain and
Compression stress respectively may be about 68.9% and 5.6 MPa）, swelling rate height (8 s basically reach swelling equilibrium), porosity is about
85.4%；Cell toxicity test result shows that medical hemostatic porous gel dressing prepared in this example has good biology
Compatibility, no cytotoxicity.
The preparation of the medical hemostatic porous gel dressing of embodiment 2
Step 1：Water-soluble human-like collagen HLC is dissolved in 10 mL distilled water and obtains the solution that concentration is 100 mg/mL,
The crosslinking agent beta-diimine Zn complex and 1 that concentration is 2% is added, each 1 mL of 2,7,8- diepoxyoctanes, adds 10mg/
ML Na2SO4Solution 2mL, it is well mixed, and is 4 with watery hydrochloric acid and sodium hydrate regulator solution pH；
Step 2：Mixed solution in step 1 is sub-packed in mould, high pressure is transferred to after keeping 2h under 60 DEG C of water baths
20min is kept to obtain gel first product in 110 DEG C in steam sterilization pan；
Step 3：First product gel in step 2 is washed 5 days with distilled water immersion, and once washing water is changed per 6h, remove salinity and
Residual cross-linker；First product gel after washing is transferred in high-pressure steam sterilizing pan and continues 110 DEG C of 2 h of holding, then again
Washed 2 days with distilled water immersion, remove residual cross-linker, control the residual total amount of crosslinking agent to be less than 2 μ g/g, obtained porous wet solidifying
Glue sample.
Step 4：Wet gel in rapid three is subjected to vacuum freeze drying after -80 DEG C of h of pre-freeze 3 and carries out Co-60 spokes
According to after sterilizing i.e. obtain sterile medical hemostatic porous gel dressing finished product.
The medical hemostatic gel of the sterile medical hemostatic porous gel dressing of gained and gained in embodiment 1 in the embodiment
Dressing physicochemical property is similar with biology performance.
The preparation of the medical hemostatic porous gel dressing of embodiment 3
Step 1：Water-soluble human-like collagen HLC is dissolved in 20 mL distilled water and obtains the solution that concentration is 200 mg/mL,
The crosslinking agent beta-diimine Zn complex and 1 that concentration is 1% is added, each 4 mL of 2,7,8- diepoxyoctanes, adds 10mg/
ML NaH2PO4Solution 2mL, it is well mixed, and is 5.5 with watery hydrochloric acid and sodium hydrate regulator solution pH；
Step 2：Mixed solution in step 1 is sub-packed in mould, high steam is transferred to after keeping 2h under 70 DEG C of environment
20min is kept to obtain gel first product in 121 DEG C in autoclave；
Step 3：First product gel in step 2 is washed 4 days with distilled water immersion, and once washing water is changed per 6h, remove salinity and
Residual cross-linker；First product gel after washing is transferred in high-pressure steam sterilizing pan and continues 121 DEG C of 3 h of holding, then again
Washed 1 day with distilled water immersion, remove residual cross-linker, control the residual total amount of crosslinking agent to be less than 2 μ g/g, obtained porous wet solidifying
Glue sample；
Step 4：Wet gel in supercritical carbon dioxide seasoning drying steps three, super porous xerogel sample is prepared,
Co-60 irradiation sterilizations are carried out to dry-eye disease, you can obtain sterile medical hemostatic porous gel dressing finished product.
The medical hemostatic gel of the sterile medical hemostatic porous gel dressing of gained and gained in embodiment 1 in the embodiment
Dressing physicochemical property is similar with biology performance.
The hemostasis experiment of the medical hemostatic porous gel dressing of embodiment 4
Selection NZw is experimental animal, establishes rabbit ear traumatic bleeding and liver trauma Hemorrhage Model, and from the present invention
The hemostasis gel dressing of gained carries out hemostasis contrast test with the styptic sponge of two kinds of brands of in the market A, B in example 1.
Fig. 5 illustrates to stop blooding to obtained sterile medical hemostatic porous gel dressing in present example 1 to rabbit ear（a-c）
With the effect of liver hemostasis (d-f), do not permeated not only after can significantly seeing material hemostasis by figure, and do not occur with tissue
Adhesion.Liver is carried out using the styptic sponge of two kinds of brands of A, B of main flow and stops blooding contrast and experiment as shown in fig. 6, can see
It is longer to go out the bleeding stopping period that two kinds of selected brand hemostatic materials have been permeated and needed by bleeding completely substantially.The process of experiment
In show in example 1 that prepared sterile medical hemostatic porous gel dressing is respectively 20- to the bleeding stopping period of rabbit ear and liver
30 s and 30-40 s, and the two kinds of brand styptic sponges of A, B chosen are about 80-90s to the bleeding stopping period of rabbit liver.
The above results show that hemostasis gel prepared by the present invention has excellent hemostatic function, are mainly due to the material
Porosity is high, and aperture is homogeneous and connectivity is good, can quickly absorb the moisture in blood, the haemocyte in blood is condensed in material
Expect surface, block broken blood vessels, realize quick-acting haemostatic powder, promote wound healing, especially suitable for infiltration caused by surgical procedure
Bled profusely caused by property bleeding and arteriorrhexis.Further, since material aperture itself is small, thus also there is bacteriostasis antibiosis and prevent
The only characteristic of wound infection.
Present disclosure is not limited to cited by above-mentioned case study on implementation, and those of ordinary skill in the art are by reading the present invention
Specification and any equivalent conversion taken technical solution of the present invention, the adjustment for not changing general principle are the present invention's
Claim is covered.
Claims (7)
- A kind of 1. preparation method of new medical hemostasis gel dressing, it is characterised in that：By the protein-based people of water-soluble macromolecule Collagen is dissolved in water, adds mass ratio as 3:1-1:3 multiple crosslinking agent beta-diimine Zn complex and 1,2,7,8- bis- The aqueous solution and inorganic salt solution of octylene oxide are well mixed, and it is 2-5.5 to adjust solution ph, is placed in water bath Carry out cross-linking reaction and obtain saliferous hydrogel；High steam processs is carried out to the saliferous hydrogel after crosslinking and distilled water immersion is washed Wash, remove inorganic salts and residual monomer crosslinked dose, and be dried with Co-60 sterilization treatments, that is, obtain a kind of new medical Hemostasis gel dressing.
- 2. the preparation method of new medical hemostasis gel dressing according to claim 1, it is characterised in that：Human-like collagen The concentration of solution is 50-400 mg/mL.
- 3. the preparation method of new medical hemostasis gel dressing according to claim 1, it is characterised in that：Described inorganic salts It is dense selected from sodium chloride, sodium phosphate, dibastic sodium phosphate, ammonium sulfate, ammonium nitrate, potassium nitrate, potassium sulfate, potassium chloride, inorganic salt solution Spend for 10-350 mg/mL, the ratio that inorganic salt solution adds volume and protein solution volume is 1:2-1:20.
- 4. the preparation method of new medical hemostasis gel dressing according to claim 1, it is characterised in that：Crosslinking agent β-two is sub- Amine Zn complex solution and 1, the mass percent concentration of 2,7,8- diepoxyoctane solution is 0.1-5.0%, preferably 0.5- 2.0%, volume is the 1-20% of protein solution volume, preferably 5-10%.
- 5. the preparation method of new medical hemostasis gel dressing according to claim 1, it is characterised in that：Reaction solution acid-base value It is 2-5.5 to regulate and control its pH value with hydrochloric acid and sodium hydroxide solution；The temperature of cross-linking reaction can be 40-80 DEG C, retention time 0.5- 5h, preferably 1-3 h.
- 6. the preparation method of new medical hemostasis gel dressing according to claim 1, it is characterised in that：Above-mentioned two sub-high pressure Steam treatment and distilled water immersion washing in, for the first time by sample at 110-121 DEG C retention time 5-30min, preferably keep Time 10-20min, then ultra-pure water washing by soaking 2-5 days；Sample is kept into 1-3 h at 110-121 DEG C for the second time, preferably protected Time 1.5-2.5h is held, then ultra-pure water washing by soaking 1-3 days, control the residual total amount of crosslinking agent to be less than 2 μ g/g.
- 7. the preparation method of new medical hemostasis gel dressing according to claim 1, it is characterised in that：The new medical The drying means of hemostasis gel dressing can be vacuum freeze-drying method or supercritical carbon dioxide seasoning.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108273122A (en) * | 2018-02-22 | 2018-07-13 | 江苏创铭医疗器械有限公司 | A kind of recombined collagen hydrogel wound dressing and its preparation method and application |
| CN110452413B (en) * | 2019-07-08 | 2021-05-18 | 南京中富先农生物科技有限公司 | Collagen cross-linking agent composition and application thereof |
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