A kind of microorganism and the compound distiller's yeast of enzyme preparation
Technical field
The present invention relates to food processing and brewing technical field, more particularly to a kind of microorganism and the compound wine of enzyme preparation
It is bent.
Background technology
Distiller's yeast, it is a kind of liquor-making raw material made of mouldy cereal, by a large amount of enzymes contained therein by cereal materialses
Diastatic fermentation is into wine.
Traditional distiller's yeast is more to be made up using wheat and rice of raw material, is also had and is made by raw material of other crops such as sorghum, corn
Distiller's yeast, for example, application for a patent for invention CN201611069665.3 disclosed in " a kind of distiller's yeast brewage process for improving distillation yield "
A kind of as made from sorghum, corn, rice, glutinous rice, wheat and buckwheat distiller's yeast.
The processing method of traditional distiller's yeast mainly has following two:(1) grain raw material immersion → crushing → addition drying and crushing
Chinese herbal medicine powder afterwards mixes and stirs → is inoculated with female song → base, wrapping up in powder → and enters bent room culture → go out bent room → drying → Cheng Qu;(2) rice
Fine ground → plus koji → plus boils ground rice and mixed and stirred and → step on song and cut song → song → and light a fire into bent room → pendulum song → shutdown insulation → ventilation
→ light → band door → shutdown is made a fire → increases firepower and dries song → goes out bent room → Cheng Qu.
Above-mentioned traditional handicraft still has the defects of certain, for example, technical sophistication is not easy to grasp, it is necessary to build bent room, during koji-making
Grain consumption is big, koji-making time length, often needs 35-45 days, and labor intensity is larger during koji-making, waste of manpower and material resources.In addition,
When the distiller's yeast that the technique is made is used to make wine, fermentation time is longer, about needs 28-40 days, and distillation yield is low, only 35-40%.
At present, the technical staff of liquor industry also has developed some new distiller's yeasts, for example, application for a patent for invention
Disclose a kind of preparation method of new-type distiller's yeast in CN201510937794.9 " preparation method of new-type distiller's yeast ", the distiller's yeast by
Aspergillus flavus koji, tanekoji of Rhizopus oryzae, aspergillus niger koji, distillery yeast and aroma-producing yeasts are mixed to prepare.But the wine brewing of new distiller's yeast
Effect is simultaneously imprecise, therefore different formula compositions, is sought efficient and stabilization new to the influence highly significant for effect of making wine
Development of the distiller's yeast formula for liquor industry has important practical significance.
For the present invention on the basis of existing distiller's yeast, research and development obtain a kind of new microorganism and the compound distiller's yeast of enzyme preparation,
Compared with common traditional distiller's yeast, higher distillation yield can be obtained using microorganism of the present invention and the compound distiller's yeast of enzyme preparation, together
When can significantly shorten fermentation time during wine brewing, and the compound distiller's yeast material composition letter of microorganism of the present invention and enzyme preparation
It is single, it is easy to Quality Control, is substantially reduced compared to traditional distiller's yeast production cost.
The content of the invention
The present invention is intended to provide a kind of microorganism for effectively improving distillation yield and the compound distiller's yeast of enzyme preparation, wine of the present invention
Bent raw material composition is simple, cost is cheap, and production technology is easy to be quick, is easy to industrialized production.Made wine using distiller's yeast of the present invention,
Fermentation time is short, and distiller's yeast dosage is few, and can significantly improve distillation yield.
The invention provides a kind of microorganism and the compound distiller's yeast of enzyme preparation, it is characterised in that it is by following weight parts
Made of raw material:
Make wine active dry yeasr 8-12, rhizopus koji 14-16, black mold 60-62, cellulase 2-3, lactobacillus acidophilus 1.5-
1.6, beta amylase 4-5, raw fragrant active dry yeasr 1.5-2.5;
The microorganism is made with the compound distiller's yeast of enzyme preparation by following steps:
Will wine brewing active dry yeasr, rhizopus koji, black mold, cellulase, lactobacillus acidophilus, beta amylase and raw fragrant active ferment
Stir, crushed under the conditions of 12-18 DEG C, gained powder crosses 60 mesh sieves, is got product after vacuum packaging after mother's mixing.
The parts by weight of above-mentioned raw materials are preferred:
Wine brewing active dry yeasr 8, rhizopus koji 14, black mold 60, cellulase 2, lactobacillus acidophilus 1.5, beta amylase 4 are raw
Fragrant active dry yeasr 1.5.
The parts by weight of above-mentioned raw materials are further preferably:
Wine brewing active dry yeasr 12, rhizopus koji 16, black mold 62, cellulase 3, lactobacillus acidophilus 1.6, beta amylase 5 are raw
Fragrant active dry yeasr 2.5.
Further, microorganism of the present invention and the raw material of the compound distiller's yeast of enzyme preparation, which is made, also includes following weight parts:
Oxidoreducing enzyme 0.6-0.8.
Preferably, another microorganism of the invention and the compound distiller's yeast of enzyme preparation, are by the raw material system of following weight parts
Into:
Wine brewing active dry yeasr 8, rhizopus koji 14, black mold 60, cellulase 2, lactobacillus acidophilus 1.5, beta amylase 4 are raw
Fragrant active dry yeasr 1.5, oxidoreducing enzyme 0.6.
Further, microorganism of the present invention and the raw material of the compound distiller's yeast of enzyme preparation, which is made, also includes following weight parts:
Nitrifier 2.2-2.5.
Preferably, another microorganism of the invention and the compound distiller's yeast of enzyme preparation, are by the raw material system of following weight parts
Into:
Wine brewing active dry yeasr 8, rhizopus koji 14, black mold 60, cellulase 2, lactobacillus acidophilus 1.5, beta amylase 4 are raw
Fragrant active dry yeasr 1.5, nitrifier 2.2.
Moreover, it relates to a kind of microorganism and application of the compound distiller's yeast of enzyme preparation in brewing industry.
Contain cellulase in distiller's yeast composition of raw materials of the present invention, the few fibers in grain can be converted into fermentable sugar
And protein, distillation yield can not only be improved, and the protein content in vinasse can be increased;Adding lactobacillus acidophilus can increase
While adding distiller's yeast product stability, wine brewing distillation yield is improved;Beta amylase is added, can be by the maltose isomery in grain into Portugal
Grape sugar, and then it is converted into alcohol;The addition of raw fragrant active dry yeasr, can retain and increase the fragrance component of white wine, improve white wine mouth
Sense.
In addition, adding oxidoreducing enzyme, the amylopectin that can enable to be difficult to convert aoxidizes, and improves starch utilization ratio;Add
Enter nitrifier and can further improve distillation yield in fermentation process.
Compared with existing distiller's yeast product, microorganism of the present invention has the characteristics that and excellent with the compound distiller's yeast of enzyme preparation
Point:
(1) grain composition is free of in composition of raw materials, is all made up of microorganism and enzyme preparation, simple production process, cost
It is cheap;
(2) it is used for when producing white wine, brewing process can be simplified, making before raw material fermentation need not first boiling ripe material, you can
Directly plus underwater koji fermentation, fermentation can be normally carried out under normal temperature and low temperature;
(3) due to multiple-microorganism and the synergy of enzyme preparation so that starch utilization ratio greatly improves, the shallow lake in raw material
Powder utilization rate reaches more than 98%, and the fiber in grain can be converted into fermentable sugar and protein;Albumen in vinasse
Matter content significantly improves, and after testing, 18 kinds of amino acid and 4 kinds of vitamins is contained in vinasse, is preferable inexpensive feedstuff;
(4) alcohol product is brewed using distiller's yeast of the present invention, fermentation time is short, and distiller's yeast dosage is small, and is remarkably improved out wine
Rate.
Embodiment
Illustrate embodiments of the present invention below by way of specific instantiation, those skilled in the art can be by this specification
Disclosed content understands other advantages and effect of the present invention easily.The present invention can also pass through specific realities different in addition
The mode of applying is embodied or practiced, the various details in this specification can also be based on different viewpoints with application, without departing from
Various modifications or alterations are carried out under the spirit of the present invention.
Before the specific embodiment of the invention is further described, it should be appreciated that protection scope of the present invention is not limited to down
State specific specific embodiment;It is also understood that the term used in the embodiment of the present invention is specific specific in order to describe
Embodiment, the protection domain being not intended to be limiting of the invention.
When embodiment provides number range, it should be appreciated that except non-invention is otherwise noted, two ends of each number range
Any one numerical value can be selected between point and two end points.Unless otherwise defined, in the present invention all technologies for using and
Scientific terminology is identical with the meaning that those skilled in the art of the present technique are generally understood that.Except used in embodiment specific method, equipment,
Outside material, according to grasp of the those skilled in the art to prior art and the record of the present invention, it can also use and this
Any method, equipment and the material of the similar or equivalent prior art of method, equipment described in inventive embodiments, material come real
The existing present invention.
Embodiment 1
A kind of microorganism and the compound distiller's yeast of enzyme preparation, are made of the raw material of following weight parts:
Wine brewing active dry yeasr 8, rhizopus koji 14, black mold 60, cellulase 2, lactobacillus acidophilus 1.5, beta amylase 4 are raw
Fragrant active dry yeasr 1.5.
Preparation method is as follows:
Will wine brewing active dry yeasr, rhizopus koji, black mold, cellulase, lactobacillus acidophilus, beta amylase and raw fragrant active ferment
Stir, crushed under the conditions of 12-18 DEG C, gained powder crosses 60 mesh sieves, is got product after vacuum packaging after mother's mixing.
Embodiment 2
A kind of microorganism and the compound distiller's yeast of enzyme preparation, are made of the raw material of following weight parts:
Wine brewing active dry yeasr 12, rhizopus koji 16, black mold 62, cellulase 3, lactobacillus acidophilus 1.6, beta amylase 5 are raw
Fragrant active dry yeasr 2.5.
Preparation method is as follows:
Will wine brewing active dry yeasr, rhizopus koji, black mold, cellulase, lactobacillus acidophilus, beta amylase and raw fragrant active ferment
Stir, crushed under the conditions of 12-18 DEG C, gained powder crosses 60 mesh sieves, is got product after vacuum packaging after mother's mixing.
Embodiment 3
A kind of microorganism and the compound distiller's yeast of enzyme preparation, are made of the raw material of following weight parts:
Wine brewing active dry yeasr 8, rhizopus koji 14, black mold 60, cellulase 2, lactobacillus acidophilus 1.5, beta amylase 4 are raw
Fragrant active dry yeasr 1.5, oxidoreducing enzyme 0.6.
Preparation method is as follows:
Will wine brewing active dry yeasr, rhizopus koji, black mold, cellulase, lactobacillus acidophilus, beta amylase, raw fragrant active ferment
Stir, crushed under the conditions of 12-18 DEG C, gained powder crosses 60 mesh sieves, vacuum packet after the mixing of female and oxidoreducing enzyme
Got product after dress.
Embodiment 4
A kind of microorganism and the compound distiller's yeast of enzyme preparation, are made of the raw material of following weight parts:
Wine brewing active dry yeasr 8, rhizopus koji 14, black mold 60, cellulase 2, lactobacillus acidophilus 1.5, beta amylase 4 are raw
Fragrant active dry yeasr 1.5, nitrifier 2.2.
Preparation method is as follows:
Will wine brewing active dry yeasr, rhizopus koji, black mold, cellulase, lactobacillus acidophilus, beta amylase, raw fragrant active ferment
Stir, crushed under the conditions of 12-18 DEG C, gained powder crosses 60 mesh sieves, after vacuum packaging after the mixing of female and nitrifier
Get product.
Comparative example
A kind of microorganism and the compound distiller's yeast of enzyme preparation, are made of the raw material of following weight parts:
Wine brewing active dry yeasr 8, rhizopus koji 14, black mold 60, cellulase 2, beta amylase 4, raw fragrant active dry yeasr 1.5,
Oxidoreducing enzyme 0.6.
Preparation method is as follows:
Will wine brewing active dry yeasr, rhizopus koji, black mold, cellulase, beta amylase, raw fragrant active dry yeasr and redox
Stir, crushed under the conditions of 12-18 DEG C, gained powder crosses 60 mesh sieves, is got product after vacuum packaging after enzyme mixing.
Distillation yield is tested
Using rice as brewing materials, the original of difference testing example 1-4 distiller's yeasts, comparative example distiller's yeast and commercially available traditional distiller's yeast
Expect distillation yield.
It is following (each group distiller's yeast brewage condition completely the same) to brewage condition:
Pressure:Standard atmospheric pressure;
Temperature:20℃;
Fermentation time:21 days.
Liquor ratio of raw material calculation formula:
Liquor ratio of raw material test result is as shown in table 1 below after the fermentation of each group distiller's yeast.
The liquor ratio of raw material test result of each group distiller's yeast of table 1
Group |
Raw material total burn-off (kg) |
Former wine yield (kg) |
Liquor ratio of raw material (%) |
Commercially available distiller's yeast |
100 |
23.5 |
23.5 |
Embodiment 1 |
100 |
72.4 |
72.4 |
Embodiment 2 |
100 |
77.1 |
77.1 |
Embodiment 3 |
100 |
76.6 |
76.6 |
Embodiment 4 |
100 |
87.9 |
87.9 |
Comparative example |
100 |
58.9 |
58.9 |
It is can be seen that by the distillation yield test result of each group distiller's yeast in table 1 after identical fermentation time, the present invention
The liquor ratio of raw material of distiller's yeast (embodiment 1-4) is significantly larger than current commercially available traditional distiller's yeast.Compared with comparative example group distiller's yeast, each reality
Apply that the liquor ratio of raw material of example group distiller's yeast is also considerably higher, so as to confirm that the formula of embodiment group distiller's yeast composition is better than comparative example
Group.Especially 4 groups of distiller's yeasts of embodiment, due to adding nitrifier in formula, it is more prominent that it improves the effect of distillation yield.
The preferred embodiments of the disclosure and embodiment are explained in detail above, but the present invention is not limited to
The above-described embodiment and examples, in those skilled in the art's possessed knowledge, the present invention can also not departed from
Various changes can be made on the premise of design.