CN107345182A - A kind of microorganism and the compound distiller's yeast of enzyme preparation - Google Patents

A kind of microorganism and the compound distiller's yeast of enzyme preparation Download PDF

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Publication number
CN107345182A
CN107345182A CN201710625153.9A CN201710625153A CN107345182A CN 107345182 A CN107345182 A CN 107345182A CN 201710625153 A CN201710625153 A CN 201710625153A CN 107345182 A CN107345182 A CN 107345182A
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yeast
distiller
microorganism
enzyme preparation
compound
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关欣
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Beijing Wine Cube Food Machinery Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12GWINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00Preparation of other alcoholic beverages
    • C12G3/02Preparation of other alcoholic beverages by fermentation

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  • Organic Chemistry (AREA)
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  • Health & Medical Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
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  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to food processing and brewing technical field, more particularly to a kind of microorganism and the compound distiller's yeast of enzyme preparation.Microorganism of the present invention is prepared with the compound distiller's yeast of enzyme preparation using active dry yeasr of making wine, rhizopus koji, black mold, cellulase, lactobacillus acidophilus, beta amylase, raw fragrant active dry yeasr as raw material, and oxidoreducing enzyme and nitrifier are may also include in the raw material of distiller's yeast of the present invention.Compared with common distiller's yeast, higher distillation yield can be obtained using distiller's yeast of the present invention, while can significantly shorten the fermentation time during wine brewing, and distiller's yeast material composition of the present invention is simple, production technology is simple and easy to operate, is substantially reduced compared to traditional distiller's yeast production cost.

Description

A kind of microorganism and the compound distiller's yeast of enzyme preparation
Technical field
The present invention relates to food processing and brewing technical field, more particularly to a kind of microorganism and the compound wine of enzyme preparation It is bent.
Background technology
Distiller's yeast, it is a kind of liquor-making raw material made of mouldy cereal, by a large amount of enzymes contained therein by cereal materialses Diastatic fermentation is into wine.
Traditional distiller's yeast is more to be made up using wheat and rice of raw material, is also had and is made by raw material of other crops such as sorghum, corn Distiller's yeast, for example, application for a patent for invention CN201611069665.3 disclosed in " a kind of distiller's yeast brewage process for improving distillation yield " A kind of as made from sorghum, corn, rice, glutinous rice, wheat and buckwheat distiller's yeast.
The processing method of traditional distiller's yeast mainly has following two:(1) grain raw material immersion → crushing → addition drying and crushing Chinese herbal medicine powder afterwards mixes and stirs → is inoculated with female song → base, wrapping up in powder → and enters bent room culture → go out bent room → drying → Cheng Qu;(2) rice Fine ground → plus koji → plus boils ground rice and mixed and stirred and → step on song and cut song → song → and light a fire into bent room → pendulum song → shutdown insulation → ventilation → light → band door → shutdown is made a fire → increases firepower and dries song → goes out bent room → Cheng Qu.
Above-mentioned traditional handicraft still has the defects of certain, for example, technical sophistication is not easy to grasp, it is necessary to build bent room, during koji-making Grain consumption is big, koji-making time length, often needs 35-45 days, and labor intensity is larger during koji-making, waste of manpower and material resources.In addition, When the distiller's yeast that the technique is made is used to make wine, fermentation time is longer, about needs 28-40 days, and distillation yield is low, only 35-40%.
At present, the technical staff of liquor industry also has developed some new distiller's yeasts, for example, application for a patent for invention Disclose a kind of preparation method of new-type distiller's yeast in CN201510937794.9 " preparation method of new-type distiller's yeast ", the distiller's yeast by Aspergillus flavus koji, tanekoji of Rhizopus oryzae, aspergillus niger koji, distillery yeast and aroma-producing yeasts are mixed to prepare.But the wine brewing of new distiller's yeast Effect is simultaneously imprecise, therefore different formula compositions, is sought efficient and stabilization new to the influence highly significant for effect of making wine Development of the distiller's yeast formula for liquor industry has important practical significance.
For the present invention on the basis of existing distiller's yeast, research and development obtain a kind of new microorganism and the compound distiller's yeast of enzyme preparation, Compared with common traditional distiller's yeast, higher distillation yield can be obtained using microorganism of the present invention and the compound distiller's yeast of enzyme preparation, together When can significantly shorten fermentation time during wine brewing, and the compound distiller's yeast material composition letter of microorganism of the present invention and enzyme preparation It is single, it is easy to Quality Control, is substantially reduced compared to traditional distiller's yeast production cost.
The content of the invention
The present invention is intended to provide a kind of microorganism for effectively improving distillation yield and the compound distiller's yeast of enzyme preparation, wine of the present invention Bent raw material composition is simple, cost is cheap, and production technology is easy to be quick, is easy to industrialized production.Made wine using distiller's yeast of the present invention, Fermentation time is short, and distiller's yeast dosage is few, and can significantly improve distillation yield.
The invention provides a kind of microorganism and the compound distiller's yeast of enzyme preparation, it is characterised in that it is by following weight parts Made of raw material:
Make wine active dry yeasr 8-12, rhizopus koji 14-16, black mold 60-62, cellulase 2-3, lactobacillus acidophilus 1.5- 1.6, beta amylase 4-5, raw fragrant active dry yeasr 1.5-2.5;
The microorganism is made with the compound distiller's yeast of enzyme preparation by following steps:
Will wine brewing active dry yeasr, rhizopus koji, black mold, cellulase, lactobacillus acidophilus, beta amylase and raw fragrant active ferment Stir, crushed under the conditions of 12-18 DEG C, gained powder crosses 60 mesh sieves, is got product after vacuum packaging after mother's mixing.
The parts by weight of above-mentioned raw materials are preferred:
Wine brewing active dry yeasr 8, rhizopus koji 14, black mold 60, cellulase 2, lactobacillus acidophilus 1.5, beta amylase 4 are raw Fragrant active dry yeasr 1.5.
The parts by weight of above-mentioned raw materials are further preferably:
Wine brewing active dry yeasr 12, rhizopus koji 16, black mold 62, cellulase 3, lactobacillus acidophilus 1.6, beta amylase 5 are raw Fragrant active dry yeasr 2.5.
Further, microorganism of the present invention and the raw material of the compound distiller's yeast of enzyme preparation, which is made, also includes following weight parts: Oxidoreducing enzyme 0.6-0.8.
Preferably, another microorganism of the invention and the compound distiller's yeast of enzyme preparation, are by the raw material system of following weight parts Into:
Wine brewing active dry yeasr 8, rhizopus koji 14, black mold 60, cellulase 2, lactobacillus acidophilus 1.5, beta amylase 4 are raw Fragrant active dry yeasr 1.5, oxidoreducing enzyme 0.6.
Further, microorganism of the present invention and the raw material of the compound distiller's yeast of enzyme preparation, which is made, also includes following weight parts: Nitrifier 2.2-2.5.
Preferably, another microorganism of the invention and the compound distiller's yeast of enzyme preparation, are by the raw material system of following weight parts Into:
Wine brewing active dry yeasr 8, rhizopus koji 14, black mold 60, cellulase 2, lactobacillus acidophilus 1.5, beta amylase 4 are raw Fragrant active dry yeasr 1.5, nitrifier 2.2.
Moreover, it relates to a kind of microorganism and application of the compound distiller's yeast of enzyme preparation in brewing industry.
Contain cellulase in distiller's yeast composition of raw materials of the present invention, the few fibers in grain can be converted into fermentable sugar And protein, distillation yield can not only be improved, and the protein content in vinasse can be increased;Adding lactobacillus acidophilus can increase While adding distiller's yeast product stability, wine brewing distillation yield is improved;Beta amylase is added, can be by the maltose isomery in grain into Portugal Grape sugar, and then it is converted into alcohol;The addition of raw fragrant active dry yeasr, can retain and increase the fragrance component of white wine, improve white wine mouth Sense.
In addition, adding oxidoreducing enzyme, the amylopectin that can enable to be difficult to convert aoxidizes, and improves starch utilization ratio;Add Enter nitrifier and can further improve distillation yield in fermentation process.
Compared with existing distiller's yeast product, microorganism of the present invention has the characteristics that and excellent with the compound distiller's yeast of enzyme preparation Point:
(1) grain composition is free of in composition of raw materials, is all made up of microorganism and enzyme preparation, simple production process, cost It is cheap;
(2) it is used for when producing white wine, brewing process can be simplified, making before raw material fermentation need not first boiling ripe material, you can Directly plus underwater koji fermentation, fermentation can be normally carried out under normal temperature and low temperature;
(3) due to multiple-microorganism and the synergy of enzyme preparation so that starch utilization ratio greatly improves, the shallow lake in raw material Powder utilization rate reaches more than 98%, and the fiber in grain can be converted into fermentable sugar and protein;Albumen in vinasse Matter content significantly improves, and after testing, 18 kinds of amino acid and 4 kinds of vitamins is contained in vinasse, is preferable inexpensive feedstuff;
(4) alcohol product is brewed using distiller's yeast of the present invention, fermentation time is short, and distiller's yeast dosage is small, and is remarkably improved out wine Rate.
Embodiment
Illustrate embodiments of the present invention below by way of specific instantiation, those skilled in the art can be by this specification Disclosed content understands other advantages and effect of the present invention easily.The present invention can also pass through specific realities different in addition The mode of applying is embodied or practiced, the various details in this specification can also be based on different viewpoints with application, without departing from Various modifications or alterations are carried out under the spirit of the present invention.
Before the specific embodiment of the invention is further described, it should be appreciated that protection scope of the present invention is not limited to down State specific specific embodiment;It is also understood that the term used in the embodiment of the present invention is specific specific in order to describe Embodiment, the protection domain being not intended to be limiting of the invention.
When embodiment provides number range, it should be appreciated that except non-invention is otherwise noted, two ends of each number range Any one numerical value can be selected between point and two end points.Unless otherwise defined, in the present invention all technologies for using and Scientific terminology is identical with the meaning that those skilled in the art of the present technique are generally understood that.Except used in embodiment specific method, equipment, Outside material, according to grasp of the those skilled in the art to prior art and the record of the present invention, it can also use and this Any method, equipment and the material of the similar or equivalent prior art of method, equipment described in inventive embodiments, material come real The existing present invention.
Embodiment 1
A kind of microorganism and the compound distiller's yeast of enzyme preparation, are made of the raw material of following weight parts:
Wine brewing active dry yeasr 8, rhizopus koji 14, black mold 60, cellulase 2, lactobacillus acidophilus 1.5, beta amylase 4 are raw Fragrant active dry yeasr 1.5.
Preparation method is as follows:
Will wine brewing active dry yeasr, rhizopus koji, black mold, cellulase, lactobacillus acidophilus, beta amylase and raw fragrant active ferment Stir, crushed under the conditions of 12-18 DEG C, gained powder crosses 60 mesh sieves, is got product after vacuum packaging after mother's mixing.
Embodiment 2
A kind of microorganism and the compound distiller's yeast of enzyme preparation, are made of the raw material of following weight parts:
Wine brewing active dry yeasr 12, rhizopus koji 16, black mold 62, cellulase 3, lactobacillus acidophilus 1.6, beta amylase 5 are raw Fragrant active dry yeasr 2.5.
Preparation method is as follows:
Will wine brewing active dry yeasr, rhizopus koji, black mold, cellulase, lactobacillus acidophilus, beta amylase and raw fragrant active ferment Stir, crushed under the conditions of 12-18 DEG C, gained powder crosses 60 mesh sieves, is got product after vacuum packaging after mother's mixing.
Embodiment 3
A kind of microorganism and the compound distiller's yeast of enzyme preparation, are made of the raw material of following weight parts:
Wine brewing active dry yeasr 8, rhizopus koji 14, black mold 60, cellulase 2, lactobacillus acidophilus 1.5, beta amylase 4 are raw Fragrant active dry yeasr 1.5, oxidoreducing enzyme 0.6.
Preparation method is as follows:
Will wine brewing active dry yeasr, rhizopus koji, black mold, cellulase, lactobacillus acidophilus, beta amylase, raw fragrant active ferment Stir, crushed under the conditions of 12-18 DEG C, gained powder crosses 60 mesh sieves, vacuum packet after the mixing of female and oxidoreducing enzyme Got product after dress.
Embodiment 4
A kind of microorganism and the compound distiller's yeast of enzyme preparation, are made of the raw material of following weight parts:
Wine brewing active dry yeasr 8, rhizopus koji 14, black mold 60, cellulase 2, lactobacillus acidophilus 1.5, beta amylase 4 are raw Fragrant active dry yeasr 1.5, nitrifier 2.2.
Preparation method is as follows:
Will wine brewing active dry yeasr, rhizopus koji, black mold, cellulase, lactobacillus acidophilus, beta amylase, raw fragrant active ferment Stir, crushed under the conditions of 12-18 DEG C, gained powder crosses 60 mesh sieves, after vacuum packaging after the mixing of female and nitrifier Get product.
Comparative example
A kind of microorganism and the compound distiller's yeast of enzyme preparation, are made of the raw material of following weight parts:
Wine brewing active dry yeasr 8, rhizopus koji 14, black mold 60, cellulase 2, beta amylase 4, raw fragrant active dry yeasr 1.5, Oxidoreducing enzyme 0.6.
Preparation method is as follows:
Will wine brewing active dry yeasr, rhizopus koji, black mold, cellulase, beta amylase, raw fragrant active dry yeasr and redox Stir, crushed under the conditions of 12-18 DEG C, gained powder crosses 60 mesh sieves, is got product after vacuum packaging after enzyme mixing.
Distillation yield is tested
Using rice as brewing materials, the original of difference testing example 1-4 distiller's yeasts, comparative example distiller's yeast and commercially available traditional distiller's yeast Expect distillation yield.
It is following (each group distiller's yeast brewage condition completely the same) to brewage condition:
Pressure:Standard atmospheric pressure;
Temperature:20℃;
Fermentation time:21 days.
Liquor ratio of raw material calculation formula:
Liquor ratio of raw material test result is as shown in table 1 below after the fermentation of each group distiller's yeast.
The liquor ratio of raw material test result of each group distiller's yeast of table 1
Group Raw material total burn-off (kg) Former wine yield (kg) Liquor ratio of raw material (%)
Commercially available distiller's yeast 100 23.5 23.5
Embodiment 1 100 72.4 72.4
Embodiment 2 100 77.1 77.1
Embodiment 3 100 76.6 76.6
Embodiment 4 100 87.9 87.9
Comparative example 100 58.9 58.9
It is can be seen that by the distillation yield test result of each group distiller's yeast in table 1 after identical fermentation time, the present invention The liquor ratio of raw material of distiller's yeast (embodiment 1-4) is significantly larger than current commercially available traditional distiller's yeast.Compared with comparative example group distiller's yeast, each reality Apply that the liquor ratio of raw material of example group distiller's yeast is also considerably higher, so as to confirm that the formula of embodiment group distiller's yeast composition is better than comparative example Group.Especially 4 groups of distiller's yeasts of embodiment, due to adding nitrifier in formula, it is more prominent that it improves the effect of distillation yield.
The preferred embodiments of the disclosure and embodiment are explained in detail above, but the present invention is not limited to The above-described embodiment and examples, in those skilled in the art's possessed knowledge, the present invention can also not departed from Various changes can be made on the premise of design.

Claims (8)

1. a kind of microorganism and the compound distiller's yeast of enzyme preparation, it is characterised in that it is made of the raw material of following weight parts:
Make wine active dry yeasr 8-12, rhizopus koji 14-16, black mold 60-62, cellulase 2-3, and lactobacillus acidophilus 1.5-1.6, β- Amylase 4-5, raw fragrant active dry yeasr 1.5-2.5;
The microorganism is made with the compound distiller's yeast of enzyme preparation by following steps:
Wine brewing active dry yeasr, rhizopus koji, black mold, cellulase, lactobacillus acidophilus, beta amylase and raw fragrant active dry yeasr are mixed Stir after conjunction, crushed under the conditions of 12-18 DEG C, gained powder crosses 60 mesh sieves, is got product after vacuum packaging.
2. microorganism as claimed in claim 1 and the compound distiller's yeast of enzyme preparation, wherein the parts by weight of each raw material are:
Wine brewing active dry yeasr 8, rhizopus koji 14, black mold 60, cellulase 2, lactobacillus acidophilus 1.5, beta amylase 4 are raw fragrant living Property yeast 1.5.
3. microorganism as claimed in claim 1 and the compound distiller's yeast of enzyme preparation, wherein the parts by weight of each raw material are:
Wine brewing active dry yeasr 12, rhizopus koji 16, black mold 62, cellulase 3, lactobacillus acidophilus 1.6, beta amylase 5 are raw fragrant living Property yeast 2.5.
4. microorganism as claimed in claim 1 and the compound distiller's yeast of enzyme preparation, are made the microorganism and enzyme preparation is compound The raw material of distiller's yeast also includes following weight parts:Oxidoreducing enzyme 0.6-0.8.
5. microorganism as claimed in claim 4 and the compound distiller's yeast of enzyme preparation, wherein the parts by weight of each raw material are:
Wine brewing active dry yeasr 8, rhizopus koji 14, black mold 60, cellulase 2, lactobacillus acidophilus 1.5, beta amylase 4 are raw fragrant living Property yeast 1.5, oxidoreducing enzyme 0.6.
6. microorganism as claimed in claim 1 and the compound distiller's yeast of enzyme preparation, are made the microorganism and enzyme preparation is compound The raw material of distiller's yeast also includes following weight parts:Nitrifier 2.2-2.5.
7. microorganism as claimed in claim 6 and the compound distiller's yeast of enzyme preparation, wherein the parts by weight of each raw material are:
Wine brewing active dry yeasr 8, rhizopus koji 14, black mold 60, cellulase 2, lactobacillus acidophilus 1.5, beta amylase 4 are raw fragrant living Property yeast 1.5, nitrifier 2.2.
8. microorganism and application of the compound distiller's yeast of enzyme preparation in brewing industry as described in claim any one of 1-7.
CN201710625153.9A 2017-07-27 2017-07-27 A kind of microorganism and the compound distiller's yeast of enzyme preparation Pending CN107345182A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109810837A (en) * 2019-03-09 2019-05-28 河北古顺酿酒股份有限公司 A kind of beneficial bacterium health care distiller's yeast and its manufacture craft

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1328123A (en) * 2000-06-08 2001-12-26 王志忠 Raw material fragrance-enhanced high-yield distiller's yeast and production process thereof
CN104450399A (en) * 2014-12-05 2015-03-25 天津科技大学 Method for producing rice-flavor liquor based on liquid fermentation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1328123A (en) * 2000-06-08 2001-12-26 王志忠 Raw material fragrance-enhanced high-yield distiller's yeast and production process thereof
CN104450399A (en) * 2014-12-05 2015-03-25 天津科技大学 Method for producing rice-flavor liquor based on liquid fermentation

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
周恒刚: "《白酒生产工艺学》", 31 May 1982 *
沈怡方: "《白酒生产技术全书》", 31 October 1998 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109810837A (en) * 2019-03-09 2019-05-28 河北古顺酿酒股份有限公司 A kind of beneficial bacterium health care distiller's yeast and its manufacture craft

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