CN107344751B - Nicotine-resistant anaerobic activated sludge domestication method - Google Patents
Nicotine-resistant anaerobic activated sludge domestication method Download PDFInfo
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- CN107344751B CN107344751B CN201710573701.8A CN201710573701A CN107344751B CN 107344751 B CN107344751 B CN 107344751B CN 201710573701 A CN201710573701 A CN 201710573701A CN 107344751 B CN107344751 B CN 107344751B
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- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 title claims abstract description 79
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 title claims abstract description 79
- 229960002715 nicotine Drugs 0.000 title claims abstract description 79
- 238000000034 method Methods 0.000 title claims abstract description 29
- 239000010802 sludge Substances 0.000 title claims abstract description 19
- 239000000243 solution Substances 0.000 claims abstract description 26
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 239000002689 soil Substances 0.000 claims abstract description 22
- 229910017053 inorganic salt Inorganic materials 0.000 claims abstract description 20
- 239000011259 mixed solution Substances 0.000 claims abstract description 16
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 15
- 239000001301 oxygen Substances 0.000 claims abstract description 15
- 239000000203 mixture Substances 0.000 claims abstract description 13
- 230000015556 catabolic process Effects 0.000 claims abstract description 11
- 238000006731 degradation reaction Methods 0.000 claims abstract description 11
- 238000005273 aeration Methods 0.000 claims abstract description 7
- 241000894006 Bacteria Species 0.000 claims abstract description 6
- 238000007599 discharging Methods 0.000 claims abstract description 6
- 238000007873 sieving Methods 0.000 claims abstract description 6
- 230000001502 supplementing effect Effects 0.000 claims abstract description 5
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 4
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 20
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 18
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 claims description 12
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 10
- 235000019270 ammonium chloride Nutrition 0.000 claims description 10
- 239000008103 glucose Substances 0.000 claims description 10
- 229910052757 nitrogen Inorganic materials 0.000 claims description 9
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 8
- 239000000126 substance Substances 0.000 claims description 8
- 238000007789 sealing Methods 0.000 claims description 7
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 6
- 239000011790 ferrous sulphate Substances 0.000 claims description 6
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 6
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 5
- 230000004913 activation Effects 0.000 claims description 4
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 3
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 235000015393 sodium molybdate Nutrition 0.000 claims description 3
- 239000011684 sodium molybdate Substances 0.000 claims description 3
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 3
- 229910021654 trace metal Inorganic materials 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 4
- 239000002351 wastewater Substances 0.000 description 10
- 230000008569 process Effects 0.000 description 7
- 241000208125 Nicotiana Species 0.000 description 6
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 5
- 238000012258 culturing Methods 0.000 description 4
- 230000000593 degrading effect Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 3
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- 230000009931 harmful effect Effects 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 235000019504 cigarettes Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000004537 pulping Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/34—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32
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- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
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Abstract
The invention relates to a nicotine-resistant anaerobic activated sludge domestication method, which comprises the steps of taking polluted soil containing anaerobic nicotine-resistant bacteria, sieving, crushing and adding an inorganic salt culture solution; removing dissolved oxygen in a mixture of the polluted soil and the inorganic salt culture solution; adding a nicotine-containing culture solution II into a mixture of the polluted soil and the inorganic salt culture solution every B to finally form a mixed solution II; adding the mixed solution II, artificial water distribution and active carbon into the SBR tank to form a mixed solution III, and carrying out intermittent aeration on the SBR tank; discharging the volume of 1/D of the middle upper part of the third mixed solution every other C days, supplementing manual water distribution, keeping the nicotine concentration in the SBR pool at E, repeating the step until the nicotine degradation rate reaches 60% -75% of the existing concentration value, changing the nicotine concentration of the reactor to be F, repeating the step until the F value reaches the I value, wherein F = E + GH, H = H +1, and G is a constant. The method cultures the nicotine-resistant anaerobic activated sludge with the concentration of more than 500mg/L, and achieves the effect of effectively removing COD and nicotine.
Description
Technical Field
The invention relates to a nicotine-resistant anaerobic activated sludge domestication method, and belongs to the technical field of biological treatment of high-concentration nicotine sewage.
Background
The anaerobic activated sludge is used for reducing organic substances such as COD (chemical oxygen demand), ammonia nitrogen and the like in the environment in the processes of anaerobic and alkaline anaerobic microbial population growth and reproduction and metabolism, thereby realizing the biological purification of the wastewater. The activated sludge process, one of the most widely used methods for wastewater treatment, increasingly exerts its ecological value in the treatment of wastewater resistant to degradation and toxic and harmful.
Nicotine, commonly known as nicotine, is an alkaloid substance in plants of the genus nicotiana, affecting tobacco leaf quality. A small amount of nicotine excites people, a large amount of nicotine causes dizziness and vomiting, and an adult can be fatal when taking 40-60 mg of nicotine once. According to the literature, more than 70t of waste water is generally discharged when 1t of product is produced in the papermaking process of the tobacco sheet, and the waste water has deep chroma, high COD (chemical oxygen demand) and ammonia nitrogen, complex components and comprises some toxic and harmful components. Therefore, the effective treatment of the wastewater is a problem to be solved in the tobacco industry at present.
Most enterprises adopt a process similar to pulping and papermaking wastewater treatment to treat tobacco sheet wastewater, namely primary physicochemical treatment, secondary biochemical treatment and tertiary decoloration treatment. However, the concentration of pollutants in the tobacco sheet wastewater is very high, so that the effluent often has the problems of unqualified COD (chemical oxygen demand) and chromaticity and the like. Therefore, the process for treating the wastewater by the activated sludge biological method has advantages.
According to the method for acclimatizing the anaerobic activated sludge in the high-concentration nicotine environment, nicotine-resistant strains are added into a reactor, and meanwhile, powdered activated carbon is added, so that the powdered activated carbon has the effect of adsorbing nicotine and provides attachments for the growth of microorganisms. The nicotine concentration in the reactor is gradually increased according to the stable degree of the nicotine degradation rate in the reactor. The method gradually eliminates microorganisms with weak nicotine tolerance, improves the concentration of microorganisms for effectively degrading nicotine, and improves the nicotine degradation rate of the reactor. The existing reactor for anaerobic degradation of nicotine is to implant screened saline-alkali tolerant strains into an anaerobic granular sludge system, the method is complicated in bacterium screening, new strains are implanted into a stable anaerobic sludge granular system, the original system is broken, and a microbial ecosystem is reestablished, so that the domestication time is long and the domestication time is unstable.
Disclosure of Invention
The technical problem to be solved by the invention is to overcome the defects of the prior art and provide a nicotine-resistant anaerobic activated sludge domestication method, and the activated sludge adaptive to high-concentration nicotine wastewater can be obtained by the method, so that a mode is provided for anaerobic biological treatment of the nicotine wastewater.
In order to achieve the aim, the invention provides a nicotine-resistant anaerobic activated sludge domestication method, which comprises the following steps:
taking polluted soil containing anaerobic nicotine-resistant bacteria, sieving and crushing;
adding an inorganic salt culture solution into the polluted soil;
removing dissolved oxygen in a mixture of the polluted soil and the inorganic salt culture solution;
sealing and placing a mixture of the polluted soil and the inorganic salt culture solution for A days, and adding a nicotine-containing culture solution II every other B to finally form a mixed solution II;
adding the mixed solution II, artificial water distribution and active carbon into the SBR tank to form a mixed solution III, and carrying out intermittent aeration on the SBR tank;
discharging 1/D volume of the middle upper part of the mixed solution III every other day C, supplementing manual water distribution, keeping the nicotine concentration in the SBR pool at E mg/L, repeating the step until the nicotine degradation rate reaches 60% -75% of the existing concentration value, changing the nicotine concentration of the reactor to be about F mg/L, repeating the step until the F value reaches an I value, wherein F is E + GH, H is defined as a natural number, H is H +1, and G is a constant.
Preferably, the contaminated soil is ground through a 100 mesh screen.
Preferably, the inorganic salt culture solution comprises 100mg of glucose, 100mg of ammonium chloride, 0.262g of monopotassium phosphate, 0.8g of dipotassium phosphate, 0.41g of magnesium sulfate, 0.1117g of calcium sulfate, 0.008g of ferrous sulfate and 0.0034g of sodium molybdate per 1L of the inorganic salt culture solution.
Preferably, the step of removing oxygen from the mixture of the contaminated soil and the inorganic salt culture solution comprises the following steps: introducing nitrogen for 30 minutes to remove oxygen, sealing and culturing at 30 ℃.
Preferably, a is 30 days, B is 5 days, D is 4, E is 100, F is 200, G is 100, and I is 500.
Preferably, broth II contains 100mg/L nicotine, 100mg/L glucose and 100mg/L ammonium chloride.
Preferably, the artificial water comprises 1g of glucose, 0.1g of ammonium chloride, 0.05g of monopotassium phosphate, 0.41g of magnesium sulfate, 0.1117g of calcium sulfate, 0.008g of ferrous sulfate, trace metal elements and 100mg/L of nicotine per 1L of artificial water.
Preferably, the activated carbon is 500mg/L powdered activated carbon.
Preferentially, the nutrient substance component ratio of the activation stage and the intermittent water inlet stage is COD: NH (NH)4-N：TP＝200:5:1。
Preferably, the intermittent aeration of the SBR tank comprises the following steps: nitrogen exposure was timed for 2h each day.
The invention achieves the following beneficial effects:
according to the method, the concentration and the nicotine degradation rate of the nicotine degrading bacteria are improved according to the continuous improvement of the nicotine concentration and the tolerance by enriching the anaerobic bacteria for degrading nicotine and then adding the anaerobic bacteria into a reactor; the method can culture the nicotine-resistant anaerobic activated sludge with the concentration of 500mg/L or more, adopts gradient to improve the concentration of nicotine, avoids impact load on a reactor caused by improving the concentration of nicotine, and achieves certain removal effect on COD and nicotine.
Drawings
Figure 1 is a graph of the nicotine removal effect of the present invention.
Detailed Description
The invention is further described below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.
A nicotine-resistant anaerobic activated sludge domestication method comprises the following steps:
taking polluted soil containing anaerobic nicotine-resistant bacteria, sieving and crushing;
adding an inorganic salt culture solution into the polluted soil;
removing dissolved oxygen in a mixture of the polluted soil and the inorganic salt culture solution;
sealing and placing a mixture of the polluted soil and the inorganic salt culture solution for A days, and adding a nicotine-containing culture solution II every other B to finally form a mixed solution II;
adding the mixed solution II, artificial water distribution and active carbon into the SBR tank to form a mixed solution III, and carrying out intermittent aeration on the SBR tank;
discharging 1/D volume of the middle upper part of the mixed solution III every other day C, supplementing manual water distribution, keeping the nicotine concentration in the SBR pool at E mg/L, repeating the step until the nicotine degradation rate reaches 60% -75% of the existing concentration value, changing the nicotine concentration of the reactor to be about F mg/L, repeating the step until the F value reaches an I value, wherein F is E + GH, H is defined as a natural number, H is H +1, and G is a constant.
Further, the contaminated soil is crushed by passing through a 100-mesh sieve.
Further, each 1L of the inorganic salt culture solution comprises 100mg of glucose, 100mg of ammonium chloride, 0.262g of monopotassium phosphate, 0.8g of dipotassium phosphate, 0.41g of magnesium sulfate, 0.1117g of calcium sulfate, 0.008g of ferrous sulfate and 0.0034g of sodium molybdate.
Further, the step of removing oxygen in the mixture of the polluted soil and the inorganic salt culture solution comprises the following steps: introducing nitrogen for 30 minutes to remove oxygen, sealing and culturing at 30 ℃.
Further, a is 30 days, B is 5 days, D is 4, E is 100, F is 200, G is 100, and I is 500.
Further, the second culture solution contained 100mg/L nicotine, 100mg/L glucose and 100mg/L ammonium chloride.
Further, each 1L of artificial water comprises 1g of glucose, 0.1g of ammonium chloride, 0.05g of monopotassium phosphate, 0.41g of magnesium sulfate, 0.1117g of calcium sulfate, 0.008g of ferrous sulfate, trace metal elements and 100mg/L of nicotine.
Further, the activated carbon is 500mg/L powdered activated carbon.
Furthermore, the nutrient substance component ratio of the activation stage and the intermittent water inlet stage is COD: NH (NH)4-N：TP＝200:5:1。
Further, the intermittent aeration of the SBR tank comprises the following steps: nitrogen exposure was timed for 2 hours per day.
The specific implementation content is as follows:
enrichment: taking 10g of deep soil polluted by nicotine for 50 years in a cigarette factory, crushing and sieving by a 100-mesh sieve, adding into 1L of inorganic salt culture solution, filling the mixture into a plastic bottle, introducing nitrogen for 30min to remove oxygen, sealing and placing in a 30 ℃ incubator, adding 100mg/L of nicotine, 100mg/L of glucose and 100mg/L of ammonium chloride every 5 days in the middle, and culturing for one month;
the activation stage of the reactor: in an SBR tank with an effective volume of 2L, 1L55 nicotine-resistant anaerobic bacteria liquid is added, artificial water distribution containing 100mg/L nicotine is added, 1g of powdered activated carbon is added, and nitrogen is aerated. Aerating nitrogen for 2 hours every day at regular time, removing dissolved oxygen, and simultaneously mixing the liquid in the reactor to ensure that the bacterial liquid is fully contacted with the activated carbon;
intermittent water feeding: discharging the settled 1/4 supernatant every 5 days, supplementing manual water distribution, keeping the nicotine concentration of the reactor about 100mg/L, and repeating the processes of supernatant discharge, water inlet and culture. The nicotine degradation rate in 5 days is measured to be relatively stable, namely 60% -75% of the prior initial concentration value of 100mg/L in the step is reached, the nicotine concentration of the reactor is changed to be about 200mg/L, and the processes of discharging, feeding and culturing the supernatant are repeated. Measuring that the nicotine degradation rate in 5 days is relatively stable and reaches 60% -75% of the initial concentration value in each step, repeating the steps and increasing the nicotine concentration by 100mg/L in sequence until the nicotine concentration is 500 mg/L;
2ml of water sample is collected in each water outlet, centrifuged at 10000rpm for 10min, and then sent to a high performance liquid chromatography for monitoring the nicotine concentration through a 0.22um cell filter. Nicotine is added into water in each time according to the nicotine concentration of water outlet, so that the nicotine concentration of the reactor is kept at about 100, 200, 300, 400 and 500 mg/L;
and (3) test results: referring to the drawings, wherein FIG. 1 is a graph of nicotine removal according to the present invention.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.
Claims (6)
1. A nicotine-resistant anaerobic activated sludge domestication method is characterized by comprising the following steps:
taking polluted soil containing anaerobic nicotine-resistant bacteria, sieving and crushing;
adding an inorganic salt culture solution into the polluted soil;
removing dissolved oxygen in a mixture of the polluted soil and the inorganic salt culture solution;
sealing and placing a mixture of the polluted soil and the inorganic salt culture solution for A days, and adding a nicotine-containing culture solution II every other B to finally form a mixed solution II;
adding the mixed solution II, artificial water distribution and active carbon into the SBR tank to form a mixed solution III, and carrying out intermittent aeration on the SBR tank;
discharging the volume of 1/D of the middle upper part of the mixed solution III every other C days, supplementing manual water distribution, keeping the nicotine concentration in the SBR pool at E mg/L, repeating the step until the nicotine degradation rate reaches 60% -75% of the existing concentration value, changing the nicotine concentration of the reactor to be F mg/L, repeating the step until the F value reaches the I value, F = E + GH, defining H as a natural number, adding 1 to the H value, and taking G as a constant;
sieving the polluted soil with a 100-mesh sieve and crushing;
every 1L of the inorganic salt culture solution comprises 100mg of glucose, 100mg of ammonium chloride, 0.262g of monopotassium phosphate, 0.8g of dipotassium phosphate, 0.41g of magnesium sulfate, 0.1117g of calcium sulfate, 0.008g of ferrous sulfate and 0.0034g of sodium molybdate;
the method for removing the oxygen in the mixture of the polluted soil and the inorganic salt culture solution comprises the following steps: introducing nitrogen for 30 minutes to remove oxygen, and sealing and placing at 30 ℃ for culture;
a =30 days, B =5 days, D =4, E =100, F =200, G =100, I = 500.
2. The method of claim 1, wherein the second culture medium comprises 100mg/L nicotine, 100mg/L glucose and 100mg/L ammonium chloride.
3. The method for acclimatizing nicotine-resistant anaerobic activated sludge according to claim 1, wherein 1L of artificial water comprises 1g of glucose, 0.1g of ammonium chloride, 0.05g of potassium dihydrogen phosphate, 0.41g of magnesium sulfate, 0.1117g of calcium sulfate, 0.008g of ferrous sulfate, trace metal elements and 100mg/L of nicotine.
4. The method of claim 1, wherein the activated carbon is 500mg/L powdered activated carbon.
5. The method for acclimatizing nicotine-resistant anaerobic activated sludge according to claim 1, wherein the nutrient substance composition ratio of the activation stage and the intermittent water feeding stage is COD: NH (NH)4-N：TP=200:5:1。
6. The method of claim 1, wherein the intermittent aeration of the SBR tank comprises the following steps: nitrogen exposure was timed for 2h each day.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103159325A (en) * | 2011-12-09 | 2013-06-19 | 中国科学院城市环境研究所 | Method for enriching anaerobic ammonium oxidation bacteria from common activated sludge |
| CN103910475A (en) * | 2013-12-30 | 2014-07-09 | 新疆环境工程技术有限责任公司 | Method for domestication of salt-resistant activated sludge |
| CN105271515A (en) * | 2015-11-25 | 2016-01-27 | 重庆大学 | Method for degrading benzene homologues through cooperation of anaerobic ammonium oxidation and denitrification and application of method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103159325A (en) * | 2011-12-09 | 2013-06-19 | 中国科学院城市环境研究所 | Method for enriching anaerobic ammonium oxidation bacteria from common activated sludge |
| CN103910475A (en) * | 2013-12-30 | 2014-07-09 | 新疆环境工程技术有限责任公司 | Method for domestication of salt-resistant activated sludge |
| CN105271515A (en) * | 2015-11-25 | 2016-01-27 | 重庆大学 | Method for degrading benzene homologues through cooperation of anaerobic ammonium oxidation and denitrification and application of method |
Non-Patent Citations (1)
| Title |
|---|
| 烟碱反硝化强化降解及微生物群落响应;侯轶等;《应用基础与工程科学学报》;20100630(第03期);第472-473页第1节 * |
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