CN107328745A - A kind of Determination Methods of Aflatoxins and device - Google Patents
A kind of Determination Methods of Aflatoxins and device Download PDFInfo
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- CN107328745A CN107328745A CN201710456335.8A CN201710456335A CN107328745A CN 107328745 A CN107328745 A CN 107328745A CN 201710456335 A CN201710456335 A CN 201710456335A CN 107328745 A CN107328745 A CN 107328745A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
- G01N21/45—Refractivity; Phase-affecting properties, e.g. optical path length using interferometric methods; using Schlieren methods
Abstract
The invention discloses a kind of Determination Methods of Aflatoxins and device, the Determination Methods of Aflatoxins includes：Aflatoxin in ultraviolet excitation material to be measured produces fluorescence signal, and fluorescence signal is reflected and shearing forms multiple light beams；Gather the interference pattern of multiple beam interference formation；And the differential bit phase of the corresponding multiple directions of multiple light beams is obtained according to interference pattern, and mutually obtain phase information to be measured to determine whether contain aflatoxin in material to be measured according to multiple differential bits.The present invention obtains the phase image of testing sample by interference imaging, and picture contrast can be improved compared with Ultraluminescence imaging technique, improves detection accuracy；The phase information of material to be measured is also obtained by using multiple directions difference corrugated, the degree of accuracy of phase fluctuations is further increased.
Description
Technical field
The present invention relates to technical field of optical detection, it particularly relates to a kind of Determination Methods of Aflatoxins and device.
Background technology
Aflatoxin (Aflatoxin, abbreviation AF) is to produce a kind of fungi toxic metabolite by aspergillus flavus and aspergillus parasiticus
Product, is also the toxicity found so far a most strong class mycotoxin.Aflatoxin mainly have AFB1 (aflatoxin B1),
This 5 class of AFB2, AFG1, AFG2, AFM1.Wherein, AFB1 toxicity is most strong, and its toxicity is 10 times of potassium cyanide, is the 68 of arsenic
Times, it is 416 times of melamine.Moreover, AFB1 carciongenic potencies are 75 times of dimethyl nitramine, international tumor research machine in 1993
Structure (International Agency for Research on Cancer, IARC) has just been classified as I class carcinogenic substances, its
Its 4 class is listed in II class carcinogenic substances.Not only toxicity is big for aflatoxin, content is low, and pollution range is wide, in grain, oil, tea, dry
Really, find there is aflatoxin contamination in more than the 100 kinds of agricultural product such as breast, feed, soy sauce and food.Therefore, aspergillus flavus is carried out
Toxin detection technology research is particularly significant.
The detection technique on aflatoxin mainly includes damage detection and the class of Non-Destructive Testing two at present.Damage detection skill
Art is predominantly based on materialization principle (such as：High performance liquid chromatography, liquid chromatography-mass spectrography/mass spectrography etc.), it has to sample
Destructiveness, carries out purification pretreatment etc., detection time is long, is not suitable for the industrial on-line checking of high-volume.And non-destructive testing technology
With to detection the nondestructive advantage of object, as people study focus.The non-destructive testing technology reported at present is typically
Using the detection technique of Ultraluminescence intensity image, it mainly irradiates object to be measured using long wave ultraviolet and excites visible fluorescence, and ties
Close camera and obtain visible fluorescence intensity image.There is publication number currently with the progress aflatoxin detection of this technology
CN105044062A patent discloses a kind of aflatoxin on-line measuring device and the material separation device using the device)
A kind of aflatoxin detection and sorting unit etc. are disclosed with publication number CN103234945A patent.Because this technology is direct
Obtain be visible fluorescence picture, but generally burst of ultraviolel produce fluorescence it is very weak, cause intensity image resolution ratio it is relatively low and
Noise is poor, and it can influence the accuracy of detection.
In addition, interference phase measurement technology is the interference pattern that intensity coherent superposition is formed using optical interference, and then from dry
Relate to and the related phase mehtod of object physical quantity to be measured is obtained in figure.Compared with aforementioned optical imaging technique, interfere phase measurement skill
Art has wide range, high sensitivity and high-precision feature, is used widely in precision optics detection field.2009,
Pierre Bon1 et al. deliver entitled " the Quadriwave lateral shearing of opinion in Optics Express
In interferometry for quantitative phase microscopy of living cells " papers, report
The phase image of living body biological under dim light is obtained using two-dimensional transmission grating shearing interferometer.It is relative with directly obtaining figure
Picture, phase image further increases picture contrast.However, reporting that detection means light path integrally uses transmission-type in this text
Layout structure, it is reducing light wave transmissions intensity in transmission to a certain degree, is having influence on interference pattern intensity distribution, limit a phase
The further raising of measurement accuracy.
For the above mentioned problem in correlation technique, effective solution is not yet proposed at present.
The content of the invention
For the above mentioned problem in correlation technique, the present invention proposes a kind of Determination Methods of Aflatoxins and device, passed through
Interference imaging obtains the phase image of testing sample, can improve picture contrast, improves detection accuracy.
The technical proposal of the invention is realized in this way：
According to an aspect of the invention, there is provided a kind of Determination Methods of Aflatoxins, including：Ultraviolet excitation is to be measured
Aflatoxin in material produces fluorescence signal, and fluorescence signal is reflected and shearing forms multiple light beams；Collection is more
The interference pattern of individual beam interference formation；And the differential bit phase of the corresponding multiple directions of multiple light beams is obtained according to interference pattern, and
Phase information to be measured is mutually obtained according to multiple differential bits to determine whether contain aflatoxin in material to be measured.
Preferably, the differential bit for obtaining the corresponding multiple directions of multiple light beams according to interference pattern mutually includes：Interference pattern is entered
Row Fourier transformation obtains the corresponding multiple spectrum distributions of multiple directions；Obtain many of the corresponding 1 grade of frequency spectrum of multiple spectrum distributions
Individual centre coordinate；Multiple spectrum distributions are moved at the origin of coordinates respectively according to multiple centre coordinates；And to movement after
Multiple spectrum distributions carry out inverse Fourier transform, obtain the parcel position phase of multiple directions.
Wherein, also include after the parcel position phase of multiple directions is obtained：Utilize parcel of the phase unwrapping to multiple directions
Position mutually carries out unpacking processing, to obtain the differential bit phase of multiple directions.
Preferably, fluorescence signal forms four light beams after shearing.
Preferably, mutually obtaining phase information to be measured according to multiple differential bits includes：Using position phase reconstruction technology to multiple sides
To differential bit be mutually reconstructed to obtain phase information to be measured.
Preferably, determine whether include in material to be measured containing aflatoxin：By phase information to be measured and default position phase
Information is contrasted, wherein, it is the phase information without infection aflatoxins material to preset phase information；And by comparing result with
Default discrepancy threshold compares, to determine whether contain aflatoxin in material to be measured.
According to another aspect of the present invention there is provided a kind of aflatoxin detection means, including：Illuminator, illumination
Aflatoxin in the ultraviolet excitation material to be measured that system is sent produces fluorescence signal；Reflecting system, enters to fluorescence signal
Row reflection；Reflecting grating, multiple light beams are cut into by the fluorescence signal that reflecting system is reflected back；Photoelectric detection unit, collection
The interference pattern of multiple beam interference formation；And processing unit, photoelectric detection unit is connected to, processing unit is obtained according to interference pattern
The differential bit phase of the corresponding multiple directions of multiple light beams is taken, and mutually obtains phase information to be measured to determine to treat according to multiple differential bits
Survey in material and whether contain aflatoxin.
Preferably, processing unit includes what is be linked in sequence：Spectrum distribution obtains subelement, and Fourier's change is carried out to interference pattern
Get the corresponding multiple spectrum distributions of multiple directions in return；Centre coordinate obtains subelement, obtains multiple spectrum distributions corresponding 1 grade
Multiple centre coordinates of frequency spectrum；Spectrum distribution moves subelement, is respectively moved multiple spectrum distributions according to multiple centre coordinates
To the origin of coordinates；Parcel position mutually obtains subelement, carries out inverse Fourier transform to multiple spectrum distributions after movement, obtains many
The parcel position phase in individual direction.
Wherein, processing unit also includes：Differential bit mutually obtains subelement, is connected to parcel position and mutually obtains subelement, difference
Position mutually obtains subelement and mutually carries out unpacking processing to the parcel position of multiple directions using phase unwrapping, to obtain multiple directions
Differential bit phase.
Preferably, the fluorescence signal that reflecting system is reflected back is cut into four light beams by reflecting grating.
Preferably, processing unit includes：Treat that location mutually obtains subelement, utilize difference of the phase reconstruction technology in position to multiple directions
Point position is mutually reconstructed to obtain phase information to be measured.
Preferably, processing unit includes：Contrast subunit, phase information to be measured is contrasted with default phase information,
Wherein, it is the phase information without infection aflatoxins material to preset phase information；And judgment sub-unit, by comparing result and in advance
If discrepancy threshold compare, to determine whether contain aflatoxin in material to be measured.
Preferably, reflecting grating includes：Substrate；And on the vertical and horizontal of substrate the reflecting part of array arrangement and
Phase delay reflecting part, reflecting part and phase delay reflecting part are disposed adjacent in longitudinally and laterally away, adjacent reflection
Non-reflective alternatively non-transparent part is provided between part and phase delay reflecting part, wherein, reflecting part and non-reflective alternatively non-transparent
Part is arranged on above substrate, and phase delay reflecting part is that the groove to be formed is etched to substrate.
Wherein, reflecting part and phase delay reflecting part are square, reflecting part and phase delay reflecting part
Size it is identical；Substrate is etched to form the etch depth of groove as a quarter of the mean wavelength of fluorescence signal.
The present invention obtains the phase image of testing sample by interference imaging, relative to existing Ultraluminescence imaging technique
Picture contrast can be improved, detection accuracy is improved；The position of material to be measured is also obtained by using multiple directions difference corrugated
Phase information, further increases the degree of accuracy of phase fluctuations.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to institute in embodiment
The accompanying drawing needed to use is briefly described, it should be apparent that, drawings in the following description are only some implementations of the present invention
Example, for those of ordinary skill in the art, on the premise of not paying creative work, can also be obtained according to these accompanying drawings
Obtain other accompanying drawings.
Fig. 1 is the flow chart of Determination Methods of Aflatoxins according to embodiments of the present invention；
Fig. 2 is reflective two-dimentional phase grating I according to an embodiment of the invention floor map；
Fig. 3 (a) and Fig. 3 (b) are the corrugated interference schematic diagram and its frequency spectrum of reflective two-dimentional phase grating I in Fig. 2 respectively
Figure；
Fig. 4 shows the floor map for the reflective two-dimentional phase grating II for being another embodiment of the present invention；
Fig. 5 (a) and Fig. 5 (b) are the corrugated interference schematic diagram and its frequency spectrum of reflective two-dimentional phase grating II in Fig. 4 respectively
Figure；
Fig. 6 is the schematic diagram of aflatoxin detection means according to embodiments of the present invention.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, the every other embodiment that those of ordinary skill in the art are obtained belongs to what the present invention was protected
Scope.
There is provided a kind of Determination Methods of Aflatoxins for embodiments in accordance with the present invention.
As shown in figure 1, Determination Methods of Aflatoxins according to embodiments of the present invention comprises the following steps：
Aflatoxin in S12, ultraviolet excitation material to be measured produces fluorescence signal, and fluorescence signal is reflected
Multiple light beams are formed with shearing；
S14, gathers the interference pattern of multiple beam interference formation；And
S16, the differential bit phase of the corresponding multiple directions of multiple light beams is obtained according to interference pattern, and according to multiple differential bit phases
Phase information to be measured is obtained to determine whether contain aflatoxin in material to be measured.
By means of the above-mentioned technical proposal of the present invention, the phase image of testing sample is obtained by interference imaging, relative to
Existing Ultraluminescence imaging technique can improve picture contrast, improve detection accuracy；It is also poor by using multiple directions
Wave-front division obtains the phase information of material to be measured, further increases the degree of accuracy of phase fluctuations.
At step S12, step S12 can be carried out by any device for being contemplated that and implementing.It is optional at one
Embodiment in, ultraviolet light can be sent by illuminator to excite aflatoxin in material to be measured to produce fluorescence signal,
Fluorescence signal is reflected by reflecting system (such as reflective colimated light system) again, divided finally by any appropriate having
The element (such as reflective two-dimentional phase grating) of light shearing function, the fluorescence signal that reflecting system is reflected back is cut into many
Individual light beam.Wherein it is possible to be carried out to reflecting system any appropriately configured so that fluorescence signal reflexes to above-mentioned reflecting grating.
Preferably, at step S12, fluorescence signal forms four light beams after shearing.Herein, can by point
The element progress of light shearing function is appropriately configured, to cause fluorescence signal to form four light beams after shearing.Fig. 2 shows root
According to the reflective two-dimentional phase grating I of one embodiment of the invention floor map；Fig. 4 show according to the present invention another
The reflective two-dimentional phase grating II of embodiment floor map.
Preferably, at step S16, mutually wrapped according to the differential bit that interference pattern obtains the corresponding multiple directions of multiple light beams
Include：
S161, carries out Fourier transformation to interference pattern and obtains the corresponding multiple spectrum distributions of multiple directions；
S162, obtains multiple centre coordinates of the corresponding 1 grade of frequency spectrum of multiple spectrum distributions；
Multiple spectrum distributions, are moved at the origin of coordinates by S163 respectively according to multiple centre coordinates；And
Multiple spectrum distributions after movement are carried out inverse Fourier transform by S164, obtain the parcel position phase of multiple directions.
Wherein, at step S16, also include after the parcel position phase of multiple directions is obtained：S165, utilizes phase unwrapping
Unpacking processing is mutually carried out to the parcel position of multiple directions, to obtain the differential bit phase of multiple directions.
Preferably, at step S16, mutually obtaining phase information to be measured according to multiple differential bits includes：S166, utilizes position phase
The differential bit of multiple directions is mutually reconstructed to obtain phase information to be measured for reconfiguration technique.
Preferably, at step S16, determine whether include in material to be measured containing aflatoxin：
S167, phase information to be measured is contrasted with default phase information, wherein, it is yellow without infection to preset phase information
The phase information of aspergillin material；And
Whether S168, comparing result is compared with default discrepancy threshold, to determine in material to be measured containing aspergillus flavus poison
Element.
In a specific embodiment, with reference to Fig. 2-Fig. 5 (b), the light beam that can be sent by illuminator is by reflection
Aforementioned four light beam X was formed later into reflective two-dimentional phase grating after formula colimated light system collimation1、X2、X3And X4；And in step
At rapid S14, this four light beam X1、X2、X3And X4The four direction interference pattern I that interference is formed can be by such as photoelectric detection unit
Element is acquired.In this specific embodiment, entered according to the four direction interference pattern I phase informations to be measured for obtaining material to be measured
And determine aflatoxin whether there is infection information, it can be obtained using following steps：
Step S101, Fourier transformation is carried out to interference pattern I, obtain interference pattern I spectrum distribution (F1,F2,F3And F4)；
And further from (F1,F2,F3And F4) spectrum distribution in obtain the centre coordinate (f of corresponding 1 grade of frequency spectrum respectively1,f2,f3With
f4) value；
Step S102, utilize centre coordinate (f1,f2,f3And f4) value to obtain four directions frequency spectrum (F1,F2,F3And F4)
Center moves on to coordinate points (0,0) place respectively；
Step S103, on the basis of step 102, to the frequency spectrum (F in the directions of interference pattern I tetra-1,F2,F3And F4) carry out inverse Fu
In leaf transformation, obtain four directions parcel position phaseWith
Step S104, phase unwrapping technology is utilized to the parcel in four directions position phaseWithCarry out at unpacking
Reason, obtains the differential bit phase Φ in four directions1, Φ2,Φ3And Φ4；
Step S105, four directions position phase reconstruction technology is utilized to the differential bit phase Φ in four directions1, Φ2,Φ3And Φ4Carry out
Reconstruct, can now obtain phase information Φ to be measured；
The infection aflatoxin material level phase Φ that step S106, contrast are measured from phase information Φ to be measuredHWith it is prior
Measure without infection aflatoxin material level phase ΦN, and different threshold value is set, so that infection aflatoxin can be realized
The rejecting of material.
As shown in fig. 6, embodiments in accordance with the present invention, additionally provide a kind of aflatoxin detection means, including：Illumination
Aflatoxin in system (201A, 201B), the ultraviolet excitation material 102 to be measured that illuminator (201A, 201B) is sent
Produce fluorescence signal；Reflecting system 202, reflects fluorescence signal；Reflecting grating 203, reflecting system 202 is reflected back
Fluorescence signal be cut into multiple light beams；Photoelectric detection unit 204, gathers the interference pattern of multiple beam interference formation；And place
Unit 205 is managed, photoelectric detection unit 204 is connected to, processing unit 205 obtains the corresponding multiple sides of multiple light beams according to interference pattern
To differential bit phase, and according to multiple differential bits mutually obtain phase information to be measured whether to determine in material 102 to be measured containing Huang
Aspertoxin.
Preferably, processing unit 205 includes what is be linked in sequence：Spectrum distribution obtains subelement, and interference pattern is carried out in Fu
Leaf transformation obtains the corresponding multiple spectrum distributions of multiple directions；Centre coordinate obtains subelement, obtains multiple spectrum distribution correspondences
1 grade of frequency spectrum multiple centre coordinates；Spectrum distribution moves subelement, according to multiple centre coordinates respectively by multiple spectrum distributions
It is moved at the origin of coordinates；Parcel position mutually obtains subelement, carries out inverse Fourier transform to multiple spectrum distributions after movement, obtains
To the parcel position phase of multiple directions.
Wherein, processing unit 205 also includes：Differential bit mutually obtains subelement, is connected to parcel position and mutually obtains subelement, poor
Point position mutually obtains subelement and mutually carries out unpacking processing to the parcel position of multiple directions using phase unwrapping, to obtain multiple directions
Differential bit phase.
Preferably, processing unit 205 includes：Treat that location mutually obtains subelement, using position phase reconstruction technology to multiple directions
Differential bit be mutually reconstructed to obtain phase information to be measured.
Preferably, processing unit 205 includes：Contrast subunit, by phase information to be measured and the progress pair of default phase information
Than, wherein, it is the phase information without infection aflatoxins material to preset phase information；And judgment sub-unit, by comparing result
Compared with default discrepancy threshold, to determine whether contain aflatoxin in material 102 to be measured.
Preferably, the fluorescence signal that reflecting system 202 is reflected back is cut into four light beams by reflecting grating 203.
Preferably, reflecting grating 203 includes：Substrate；And on the vertical and horizontal of substrate array arrangement reflecting part
Point b1 and phase delay reflecting part b2, reflecting part b1 and phase delay reflecting part b2 is adjacent in longitudinally and laterally away sets
Put, non-reflective alternatively non-transparent part a1 is provided between adjacent reflecting part b1 and phase delay reflecting part b2, wherein, reflection
Part b1 and non-reflective alternatively non-transparent part a1 are arranged on above substrate, and phase delay reflecting part b2 is arranged on the groove of substrate
It is interior.
Wherein, reflecting part b1 and phase delay reflecting part b2 are square, and reflecting part b1 and phase delay are anti-
The size for penetrating part b2 is identical；Substrate is etched using the etch depth for forming groove as the four of the mean wavelength of fluorescence signal
/ mono-.
As shown in Fig. 2 in one embodiment, square first length of side and second horizontally-parallel, the square length of side
With it is parallel longitudinal, first length of side and second length of side are vertical.
As shown in figure 4, in another embodiment, square first length of side with laterally in 45 ° of angles, square the
Two length of sides are in 45 ° with longitudinal direction, and first length of side and second length of side are vertical.
Specifically, above-mentioned reflecting grating 203 can be reflective two-dimentional phase grating, and be designed specifically to reflective Two-dimensional Position
Phase grating I or reflective two kinds of structures of two-dimentional phase grating II.With reference to shown in Fig. 2 and Fig. 4, include the reflecting part of array arrangement
B1 and phase delay reflecting part b2, reflecting part b1 and phase delay reflecting part b2 are disposed adjacent in longitudinally and laterally away,
Non-reflective alternatively non-transparent part a1, i.e. reflecting part b1 are provided between adjacent reflecting part b1 and phase delay reflecting part b2
It is arranged on non-reflective alternatively non-transparent part a1 above substrate, the groove internal reflection grating I that phase delay part is arranged on substrate exists
Cycle phase on two-dimensional directional is same, and non-reflective alternatively non-transparent unit and reflected light list are included in both direction a cycle
Member.Wherein, non-reflective alternatively non-transparent unit includes non-reflective alternatively non-transparent part a1 and non-reflective alternatively non-transparent part a2, reflected beam portion
Comprising direct reflecting part b1 and phase delay reflecting part b2, moreover, a1 is identical with a2 size, b1 and b2 size phase
Together, reflecting part b1 and phase delay reflecting part b2 are square, the size phase of reflecting part b1 and phase delay reflection
Together, while first length of side of the square and second horizontally-parallel, the square length of side and parallel longitudinal, first length of side and second
The length of side is vertical.Similarly, reflecting grating II is similar with reflecting grating I, and it is specifically distinguished as first length of side of square with being in laterally
45 ° of angles, square second length of side and longitudinal direction is in 45 °, and first length of side and second length of side are vertical.Reflective two-dimentional phase grating
Incident beam, using the design of above-mentioned special construction, is diffracted primarily into (+1 ,+1), (- 1 ,+1), (- 1, -1), (+1, -1) four by 203
Beam reflected light, is reduced or eliminated other level diffraction light influences.
In addition, the base material of reflecting grating 203 uses SiO2, non-reflective alternatively non-transparent part is Cr films, reflecting grating 203
In directly reflecting part b1 be Al films, and phase delay reflecting part b2 is that the substrate of reflecting grating 203 is etched and obtained, after etching
Surface is coated with Al films.
Illustrated below by taking reflecting grating I as an example, if the alternatively non-transparent part a1 and alternatively non-transparent part a2 of reflecting grating 203
Width be wa, directly reflecting part b1 and phase delay reflecting part b2 width is w in reflected beam portionb, phase prolongs
Reflecting part b2 is relative to direct reflecting part b1 bit phase delay lateTheoretical, the reflecting grating 203 according to Fourier transformation
Reflected light far-field intensity distribution can be expressed as：
Wherein, m, n are reflected light level.
It can be seen that from above-mentioned formula (1)：When direct reflecting part b1 and phase in reflecting grating I reflected beam portion
Delayed reflex part b2 phase differencesWhen, now reflecting grating I reflection 0 grade and all even number level diffraction lights disappear；
Work as wA=2wbWhen, ± 3 grades of reflecting grating I and ± 3 times order diffraction light disappear, and reflecting grating I mainly spreads out incident light wave
Penetrate into (+1 ,+1), (- 1 ,+1), (- 1, -1), (+1, -1) four beam reflected light.
Similarly, based on similar foundation, reflecting grating II also incident light wave can be mainly diffracted into (+1 ,+1), (- 1 ,+
1), (- 1, -1), (+1, -1) four beam reflected light.
In addition, phase delay reflecting part b2 etching depths are calculated as follows：
Assuming that phase delay reflecting part b2 etching depths are that h introducings position is mutually in reflecting grating 203Because phase is prolonged
The etched transmission mediums of slow reflected beam portion b2 are air, then according to the relation between optical path difference and position phase, relative to directly anti-
Light part b1 is penetrated, the bit phase delay calculation formula that phase delay reflected beam portion b2 is introduced is as follows：
Wherein, in order to reduce reflection visible fluorescence in spectrum influence, negate the mean wavelength for penetrating visible fluorescenceCarry out
Calculate.
Understood according to above analysis, obtain phase delay reflecting part b2 etching depths so as to be calculated with reference to formula (2) and be：
Therefore, reflecting grating 203 of the invention by incident fluorescence signal be mainly diffracted into (+1 ,+1), (- 1 ,+1), (-
1, -1), (+1, -1) four beam reflected light.
In summary, by means of the above-mentioned technical proposal of the present invention, it is laid out using reflective structure, it is to avoid transmission-type structure
The problem of being absorbed to week fluorescent, improves week fluorescent interference pattern picture contrast, and then improve accuracy of detection；By interfering into
Position phase images as obtaining object to be measured, relative to existing Ultraluminescence imaging technique, the phase image that the present invention is obtained can
To further increase picture contrast, detection accuracy is improved；Material to be measured is obtained by using multiple directions difference corrugated
Phase information, further increase the degree of accuracy of phase fluctuations；The reflective two-dimentional phase grating designed using special construction
Four beam reflected lights are only produced, other level diffraction light influences are reduced or eliminated, so as to improve the degree of accuracy of detection.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
God is with principle, and any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.
Claims (14)
1. a kind of Determination Methods of Aflatoxins, it is characterised in that including：
Aflatoxin in ultraviolet excitation material to be measured produces fluorescence signal, and the fluorescence signal is reflected and cut
Cut to form multiple light beams；
Gather the interference pattern of the multiple beam interference formation；And
The differential bit phase of the corresponding multiple directions of the multiple light beam is obtained according to the interference pattern, and according to multiple differential bit phases
Phase information to be measured is obtained to determine whether contain aflatoxin in the material to be measured.
2. Determination Methods of Aflatoxins according to claim 1, it is characterised in that according to being obtained the interference pattern
The differential bit of the corresponding multiple directions of multiple light beams mutually includes：
Fourier transformation is carried out to the interference pattern and obtains the corresponding multiple spectrum distributions of multiple directions；
Obtain multiple centre coordinates of the corresponding 1 grade of frequency spectrum of the multiple spectrum distribution；
The multiple spectrum distribution is moved at the origin of coordinates respectively according to the multiple centre coordinate；And
Inverse Fourier transform is carried out to the multiple spectrum distribution after movement, the parcel position phase of multiple directions is obtained.
3. Determination Methods of Aflatoxins according to claim 2, it is characterised in that obtaining the bag in the multiple direction
Wrapping up in also includes after a phase：
Unpacking processing is mutually carried out to the parcel position in the multiple direction using phase unwrapping, to obtain the difference in the multiple direction
Divide position phase.
4. Determination Methods of Aflatoxins according to claim 1, it is characterised in that
The fluorescence signal forms four light beams after the shearing.
5. Determination Methods of Aflatoxins according to claim 1, it is characterised in that mutually obtained according to the multiple differential bit
Include to phase information to be measured：
The differential bit in the multiple direction is mutually reconstructed using position phase reconstruction technology to obtain the phase information to be measured.
6. Determination Methods of Aflatoxins according to claim 1, it is characterised in that determine in the material to be measured whether
Include containing aflatoxin：
The phase information to be measured and default phase information are contrasted, wherein, the default phase information is yellow without infection
The phase information of aspergillin material；And
Comparing result is compared with default discrepancy threshold, to determine whether contain aflatoxin in the material to be measured.
7. a kind of aflatoxin detection means, it is characterised in that including：
Aflatoxin in illuminator, the ultraviolet excitation material to be measured that the illuminator is sent produces fluorescence signal；
Reflecting system, reflects the fluorescence signal；
Reflecting grating, the fluorescence signal that the reflecting system is reflected back is cut into multiple light beams；
Photoelectric detection unit, gathers the interference pattern of the multiple beam interference formation；And
Processing unit, is connected to the photoelectric detection unit, and the processing unit obtains the multiple light according to the interference pattern
The differential bit phase of the corresponding multiple directions of beam, and according to the multiple differential bit mutually obtain phase information to be measured to treat described in determining
Survey in material and whether contain aflatoxin.
8. aflatoxin detection means according to claim 7, it is characterised in that the processing unit includes order and connected
Connect：
Spectrum distribution obtains subelement, and Fourier transformation is carried out to the interference pattern and obtains the corresponding multiple frequency spectrums point of multiple directions
Cloth；
Centre coordinate obtains subelement, obtains multiple centre coordinates of the corresponding 1 grade of frequency spectrum of the multiple spectrum distribution；
Spectrum distribution moves subelement, and it is former that the multiple spectrum distribution is moved into coordinate respectively according to the multiple centre coordinate
At point；And
Parcel position mutually obtains subelement, carries out inverse Fourier transform to the multiple spectrum distribution after movement, obtains multiple sides
To parcel position phase.
9. aflatoxin detection means according to claim 8, it is characterised in that the processing unit also includes：
Differential bit mutually obtains subelement, is connected to the parcel position and mutually obtains subelement, the differential bit mutually obtains subelement profit
Unpacking processing is mutually carried out to the parcel position in the multiple direction with phase unwrapping, to obtain the differential bit in the multiple direction
Phase.
10. aflatoxin detection means according to claim 7, it is characterised in that
The fluorescence signal that the reflecting system is reflected back is cut into four light beams by the reflecting grating.
11. aflatoxin detection means according to claim 7, it is characterised in that the processing unit includes：
Treat that location mutually obtains subelement, the differential bit in the multiple direction is mutually reconstructed to obtain using position phase reconstruction technology
The phase information to be measured.
12. aflatoxin detection means according to claim 7, it is characterised in that the processing unit includes：
Contrast subunit, the phase information to be measured and default phase information are contrasted, wherein, the default phase information
For the phase information without infection aflatoxins material；And
Whether judgment sub-unit, comparing result is compared with default discrepancy threshold, to determine in the material to be measured containing Huang
Aspertoxin.
13. aflatoxin detection means according to claim 10, it is characterised in that the reflecting grating includes：Base
Bottom；And
The reflecting part of array arrangement and phase delay reflecting part, the reflecting part on the vertical and horizontal of the substrate
It is disposed adjacent with the phase delay reflecting part along the vertical and horizontal, adjacent reflecting part and phase delay reflection
Non-reflective alternatively non-transparent part is provided between part, wherein, the reflecting part and the non-reflective alternatively non-transparent part are arranged on
Above the substrate, the phase delay reflecting part is to be etched the groove to be formed to the substrate.
14. aflatoxin detection means according to claim 13, it is characterised in that the reflecting part and the phase
Position delayed reflex part is square, and the reflecting part is identical with the size of the phase delay reflecting part；To described
Substrate is etched to form the etch depth of the groove as a quarter of the mean wavelength of the fluorescence signal.
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