CN107328735A - Rape species discrimination method based on terahertz light spectral technology - Google Patents
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Abstract
The invention discloses a kind of rape species discrimination method based on terahertz light spectral technology, rape leaf sample is selected from the rape of two kinds of different cultivars respectively, the terahertz time-domain spectroscopy of reference signal and rape leaf is gathered using terahertz time-domain spectroscopy instrument；Terahertz time-domain spectroscopy is converted into Terahertz frequency domain spectra；Optical transmission spectra, refractive index spectra, absorption coefficient spectrum and extinction coefficient spectrum are extracted from Terahertz frequency domain spectra；Classification and Identification is carried out respectively to 4 kinds of optical parametric spectrum of 2 kinds of rapes after spectral manipulation, realizes that the rape species based on terahertz light spectral technology differentiates.Patent utilization terahertz light spectral technology of the present invention, study the differentiation appraisal to different cultivars rape, there is certain theory and practice directive significance to the technology platform, the optimal screening to rape variety and breeding fine-grained management for setting up rape variety Rapid identification, application prospects of the THz in agricultural, agricultural product cultivar identification field is illustrated.
Description
Technical field
The present invention relates to the kind differentiating method of rape, more particularly to a kind of rape species based on terahertz light spectral technology
Discrimination method.
Background technology
Rape is general designation of the several crop for producing oil expression seed of Cruciferae Brassica genus on agronomy.Rape
It is one of the topmost oil seed production raw material of China and industrial crops, accounts for more than the 40% of China's oil plant industrial crops gross area,
More than the 30% of total oil-bearing crops.Rape mainly produces crop as oil plant, in its rapeseed oil rich in oleic acid, linoleic acid etc. no
Saturated fatty acid, with the health-care efficacy such as prevention of cardiovascular disease and reduction serum cholesterol in humans.In China, about 15
Individual different rape variety, the rape of different cultivars has certain morphological feature, the specificity of yield traits and the difference of stability
It is different, quality can be identified by analyzing kind hereditary capacity or the identification of rape variety is carried out with other breed differences.Oil
Dish cultivar identification is the excellent rape variety of screening, guarantee yield of Brassica napus L, breed improvement and the basis of optimization, is also to realize seed
The basis of quality standardization, protection breed breeding person and user's interests.Therefore the technology for setting up rape variety Rapid identification is put down
Platform tool is of great significance.
For the identification of rape variety, PAGE argentations are generally used at present, but there is detection efficiency in this detection method
Relatively low, poor repeatability, the shortcomings of accurately can not recognize clip size；In addition, also SSR fluorescence labelings capillary electrophoresis detection skill
Art, but this technology is the fingerprint identification based on DNA, complex operation, takes time and effort；Such detection method is needed in sample preparation
Expend considerable time and effort, some waste liquid discarded objects etc. cause severe contamination to environment, and destructive test will make plant
Thing growth course can not continue, at the same long detection time can not ensure data ageing and different sample measurement data it
Between synchronism.
With the fast development of spectral technique, near infrared spectrum detection method, EO-1 hyperion detection method, Electromagnetic Wave Detection method and too
Hertz wave spectrum (Terahert, THz) detection method is widely used in floristics and differentiates research.Due to substantial amounts of macromolecular vibration and
Rotational energy level shows very strong absorption and resonance all in terahertz wave band (0.1~10THz) in terahertz wave band, and
The tera-hertz spectra of material includes abundant physical message and chemical information.For most organic-biological macromolecular, too
Hertz wave band has fingerprint characteristic, can be used for the differentiation identification of material.Avoid biology thin in addition, Terahertz photon energy is low
The photoionization damage of born of the same parents.Terahertz has stronger penetrability, can penetrate as this relatively thin biological specimen of rape leaf.Cause
This, tera-hertz spectra detection technique has certain advantage on the rape leaf for differentiating different cultivars.
The present invention is carried out using the fingerprint, high-penetration, low damaging of Terahertz Technology using terahertz light spectral technology
Cultivar identification based on rape leaf.The rape in two different cultivars in identical growing environment identical growth period is selected in experiment,
The time-domain spectroscopy of rape leaf is gathered by terahertz time-domain spectroscopy instrument transmission-type module, by Fourier transformation by time-domain spectroscopy
Be converted to frequency domain spectra, then extraction Terahertz optical transmission spectra, refractive index spectra, absorption coefficient light on the basis of frequency domain spectra
Spectrum and extinction coefficient spectrum.Then pretreatment optimization is carried out to 4 kinds of optical parametric spectrum, finally linearly sentenced using offset minimum binary
Do not analyze and identification is made a distinction to the rape of two kinds.Invention is put down to the technology for setting up rape variety Rapid identification
Platform, the optimal screening to rape variety and breeding fine-grained management have certain theory and practice directive significance.
The content of the invention
To overcome the problems of prior art, the invention provides a kind of rape product based on terahertz light spectral technology
Plant authentication method.
A kind of rape species discrimination method based on terahertz light spectral technology, comprises the following steps：
(1) rape leaf sample is selected from the rape of two kinds of different cultivars respectively, is adopted using terahertz time-domain spectroscopy instrument
Collect the terahertz time-domain spectroscopy of reference signal and rape leaf；Terahertz time-domain spectroscopy is converted into Terahertz frequency domain spectra；
(2) optical transmission spectra, refractive index spectra, absorption coefficient spectrum and extinction coefficient are extracted from Terahertz frequency domain spectra
Spectrum；And 4 kinds of optical parametric spectrum are smoothly pre-processed；
(3) class number of two kinds of rapes is entered as 1 and -1 respectively, then by class number's information respectively with it is smooth pre-
4 kinds of optical parametric spectral informations after processing carry out offset minimum binary linear discriminant analysis modeling；
(4) rape leaf of two kinds of different cultivars rapes is sample in collection such as step (1), obtains the terahertz of rape leaf
Hereby time-domain spectroscopy, brings into step (3) institute established model after being handled through step (2), carries out the discriminating of rape variety.Implement the present invention
Method can use time domain tera-hertz spectra transmission-type scanning system, and laser is Ti∶Sapphire laser femtosecond laser oscillator；Wavelength：
800nm；Repetition rate：80MHz；Frequency range：0.1~3.5THz；Pulse width：50fs；Mean power：500m W；
Preferably, select 2 rape varieties in step (1) are respectively fresh oil 6 and ridge oil 6.At 2 kinds of rapes
In identical growing environment and identical growth period.
Preferably, select in step (1) and step (4) grow fine, plant height is close, no disease and pests harm, healthy growth
Rape leaf；The rape of two kinds is in identical planting environment, identical growth period.
Carried out preferably, choosing 3 different point positions when gathering the terahertz time-domain spectroscopy of rape leaf sample every time
Collection, each point three spectrum of position repeated acquisition.The point position for gathering rape leaf terahertz time-domain spectroscopy is mesophyll part, it is to avoid
Vein part.
Preferably, the rape sample of 2 kinds in step (1) chooses the rapeseed plants in florescence, plant height about 80cm,
Select apart from ground about 60cm, grow fine, the fresh rape leaf that blade is complete, no disease and pests harm, size are close.Each collection 40
The spectrum of individual blade.For each rape leaf sample, select mesophyll position, avoid vein position, 3 different points are gathered altogether
Position, each point position repeated acquisition 3 times, gather 9 time-domain spectroscopies, this 9 spectrums are taken into the average time domain final as the blade altogether
Spectrum.The time of integration is 39.2ps, and the sampling number of time-domain spectroscopy is 1000, and temporal resolution is 39.2fs.
Preferably, in detection process, drying nitrogen is filled into pattern detection storehouse, air humidity is controlled less than 5%, and
Using the transmission scan module of terahertz time-domain spectroscopy system, obtain reference signal time-domain spectroscopy using drying nitrogen as background and
The terahertz time-domain spectroscopy of rape leaf.
The temporal resolution of terahertz time-domain spectroscopy is 39.2fs (femtosecond).
Frequency domain spectra is obtained after being fourier transformed in step (2), it is 0.1~3.5THz, frequency point to take band limits
Resolution is 25.5GHz.
Preferably, extracting the higher 0.3~2THz frequency ranges of signal to noise ratio for 4 kinds of optical parametric spectrum in step (2)
After optical transmission spectra, refractive index spectra, absorption coefficient spectrum, extinction coefficient spectrum, chosen spectrum scope is 0.3~2THz spectrum
Duan Jinhang offset minimum binaries linear discriminant analysis (PLS-LDA) is modeled.
Optical transmission spectra, refractive index spectra, absorption coefficient spectrum and extinction coefficient light are extracted based on Terahertz frequency domain spectra
The model that spectrum is extracted using THz optical parametrics, with reference to following document.
1.Duvillaret,L.；Garet,F.；Coutaz,J.L.,Highly precise determination of
optical constants and sample thickness in terahertz time-domain
spectroscopy.Appl.Opt.1999,38(2),409.
2.Duvillaret,L.；Garet,F.；Coutaz,J.L.,A reliable method for extraction
of material parameters in terahertz time-domain spectroscopy.IEEE
J.Sel.Top.Quantum Electron.2002,2(3),739-746.
3.Dorney,T.D.；Baraniuk,R.G.；Mittleman,D.M.,Material parameter
estimation with terahertz time-domain spectroscopy.Journal of the Optical
Society of America A Optics Image Science&Vision 2001,18(7),1562.
Preferably, the method for the smoothing processing is that Savitzky-Golay is smooth.To reach the purpose of denoising optimization.
Preferably, the type number difference amplitude of two kinds of rapes is 1 and -1 in step (3), then with the 4 of two kinds of rapes
Kind optical parametric spectrum carries out offset minimum binary linear discriminant analysis method and is modeled respectively.
The present invention obtains time-domain spectroscopy of two kinds of rape leafs in 0-39.2ps；Time-domain spectroscopy is subjected to Fourier transformation
Obtain the frequency spectrum in the range of 0.1~3.5THz；According to frequency spectrum, extract in the range of 0.3 higher~2THz of signal to noise ratio
Optical transmission spectra, refractive index spectra, absorption coefficient spectrum, extinction coefficient spectrum；Using 4 kinds of light in the range of 0.3~2THz
Learn class number of the parameter spectrum respectively with two kinds of rapes and carry out offset minimum binary linear discriminant analysis modeling, according to model result
Analyze the feasibility and accuracy of the rape variety authentication method based on terahertz light spectral technology.
Patent utilization terahertz light spectral technology of the present invention, studies the differentiation appraisal to different cultivars rape, to setting up
The technology platform of rape variety Rapid identification, the optimal screening to rape variety and breeding fine-grained management have certain theory
With practical advice meaning, application prospects of the THz in agricultural, agricultural product cultivar identification field is illustrated.
The sensitivity that PLS-LDA models are built based on optical transmission spectra be 0.7602, specificity be 0.8578, it is optimal most into
Fraction is that 4, accuracy is 0.875；The sensitivity for building PLS-LDA models based on refractive index spectra is that 0.8253, specificity is
0.8247th, optimal most component number is that 5, accuracy is 0.8875；The sensitivity of PLS-LDA models is built based on absorption coefficient spectrum
It is that 0.7619, optimal most component number is that 3, accuracy is 0.8375 for 0.8057, specificity；Built based on extinction coefficient spectrum
The sensitivity of PLS-LDA models is that 0.7939, specificity is that 0.7936, optimal most component number is that 3, accuracy is 0.8375.
For the rape species discrimination method based on terahertz light spectral technology, compared to it is traditional such as PAGE argentations and
SSR fluorescence labeling capillary electrophoresis detection technologies etc., tera-hertz spectra detection technique due to fingerprint characteristic, high-penetration,
Unique characteristic such as coherence, transient response, strong absorptive, low energy damaging.Therefore, tera-hertz spectra is to be used for rape
One of scientific method that species differentiates.
Brief description of the drawings
Fig. 1：In the range of 0.3~2THz under 4 kinds of optical parametric spectrum two kinds of rapes averaged spectrum.A
Optical transmission spectra；B refractive index spectras；C absorption coefficient spectrum；D extinction coefficient spectrum.
Fig. 2：Offset minimum binary linear discriminant analysis result.A optical transmission spectras；B refractive index spectras；
C absorption coefficient spectrum；D extinction coefficient spectrum.
Embodiment
Implement a kind of rape species discrimination method based on terahertz light spectral technology herein, comprise the following steps：
(1) sample prepares：Oily No. 6 the two rape varieties of fresh oil 6 and ridge are chosen, are mustard type rape, it is general logical
Cross and be visually difficult to differentiate between its kind.Two kinds of rapes are planted in greenhouse simultaneously, growing environment is that sunshine duration is about 10h, temperature
24 degree of degree, humidity 65%.Apply appropriate moisture daily, to ensure the normal growth of rape.
(2) spectra collection：Laboratory apparatus is using CIP-THz transmission scan systems, the drying nitrogen being full of into sample storehouse,
Internal system humidity is set to be less than 5%.Entirely sweep during spectrum, test constant indoor temperature 294K, relative humidity perseverance is less than 20%.First adopt
Integrate the reference spectra using nitrogen as background, 40 blades are then respectively taken from two kinds of rapes, it is desirable to be identical leaf position, high apart from ground
The roughly the same, leaf blade size of degree is close, healthy no disease and pests harm.The different point positions of each blade selection 3 (selection mesophyll part,
Avoid vein position), it is each to put position repeated acquisition 3 times.9 time-domain spectroscopies of acquisition are taken into the average Terahertz as the sample
Time-domain spectroscopy.
(3) spectral manipulation：The sample terahertz time-domain spectroscopy gathered is subjected to Fourier transformation, corresponding frequency is converted into
Domain modal data, is then carried according to Duvillaret, Timothy etc. (referring to the document recorded before such as) THz optical parametrics proposed
The model taken, calculates transmitance T (ω), refractive index n (ω), absorption coefficient (ω), the extinction coefficient k for obtaining institute's test sample product
(ω) these four main optical parametric spectrum.The band limits of extraction is 0.3~2THz, then using Savitzky-Golay side
Method carries out smoothing denoising processing to transmissivity spectrum.Model inference process is as follows：
The frequency-domain waveform of terahertz sources is E0(ω), the transmitting frequency-domain waveform as reference light that detector is directly received
Compose Eref(ω), through sample after the frequency-domain waveform that receives of detector be sample signal Esample(ω).The sample of measurement is grand
Complex refractivity index can be used by seeing optical propertyRepresent：
Wherein, n (ω) is the actual refractive index of sample, and it describes the dispersion of sample；K (ω) is extinction coefficient, it
The absorption characteristic of sample is described.ω=2 π f, f are frequency.Free space air refraction is 1, then reference spectra Eref
(ω) expression formula is：
L be terahertz pulse in the distance of free-space propagation, c is the light velocity, and the signal spectrum through sample is represented by：
Wherein d is thickness of sample, and 1/ (n (ω)+1) and 2n (ω)/(n (ω)+1) are respectively the incident sample of terahertz pulse
With the transmission coefficient of outgoing sample.Therefore, terahertz pulse passes through the transmissivity after sample to be expressed as：
Refractive index and absorption coefficient can be obtained by Fresnel relations：
Refractive index：
Absorption coefficient：
(4) model is set up：Class number's difference amplitude by two kinds of rapes is 1 and -1, then with passing through Savitzky-
4 kinds of optical parametric spectrum after the processing of Golay methods carry out offset minimum binary linear discriminant analysis modeling respectively.According to model knot
Fruit carries out the cultivar identification of two kinds of rapes, and its result is：The sensitivity for building PLS-LDA models based on optical transmission spectra is
0.7602nd, specificity is that 0.8578, optimal most component number is that 4, accuracy is 0.875；PLS-LDA is built based on refractive index spectra
The sensitivity of model is that 0.8253, specificity is that 0.8247, optimal most component number is that 5, accuracy is 0.8875；It is based on absorbing
The sensitivity that number spectrum builds PLS-LDA models is that 0.8057, specificity is that 0.7619, optimal most component number is that 3, accuracy is
0.8375；The sensitivity that PLS-LDA models are built based on extinction coefficient spectrum be 0.7939, specificity be 0.7936, it is optimal most
Component number is that 3, accuracy is 0.8375.Illustrating can accurate identification identification using 4 kinds of optical parametric spectrum of Terahertz
Go out the rape of two kinds, there is important promotional value in agricultural breeding seed selection.
Claims (8)
1. a kind of rape species discrimination method based on terahertz light spectral technology, it is characterised in that comprise the following steps：
(1) rape leaf sample is selected from the rape of two kinds of different cultivars respectively, is gathered and joined using terahertz time-domain spectroscopy instrument
Examine the terahertz time-domain spectroscopy of signal and rape leaf；Terahertz time-domain spectroscopy is converted into Terahertz frequency domain spectra；
(2) optical transmission spectra, refractive index spectra, absorption coefficient spectrum and extinction coefficient light are extracted from Terahertz frequency domain spectra
Spectrum；And 4 kinds of optical parametric spectrum are smoothly pre-processed；
(3) class number of two kinds of rapes is entered as 1 and -1 respectively, then respectively with passing through the 4 kinds of optics smoothly pre-processed
Parameter spectrum carries out offset minimum binary linear discriminant analysis modeling；
(4) rape leaf of two kinds of different cultivars rapes is sample in collection such as step (1), when obtaining the Terahertz of rape leaf
Domain spectrum, brings into step (3) institute established model after being handled through step (2), carries out the discriminating of rape variety.
2. rape species discrimination method according to claim 1, it is characterised in that select growing way in step (1) and step (4)
Well, plant height is close, no disease and pests harm, the rape leaf of healthy growth；The rape of two kinds is in identical planting environment, identical
Growth period.
3. rape species discrimination method according to claim 1, it is characterised in that the terahertz of collection rape leaf sample every time
Hereby choose 3 different point positions during time-domain spectroscopy to be acquired, each point three spectrum of position repeated acquisition.Gather rape leaf too
The point position of hertz time-domain spectroscopy is mesophyll part.
4. rape species discrimination method according to claim 1, it is characterised in that in detection process, to pattern detection storehouse
Drying nitrogen is inside filled, control air humidity is less than 5%, and using the transmission scan module of terahertz time-domain spectroscopy system, obtains
The terahertz time-domain spectroscopy of reference signal time-domain spectroscopy and rape leaf by background of drying nitrogen.
5. rape species discrimination method according to claim 1, it is characterised in that the temporal resolution of terahertz time-domain spectroscopy
For 39.2fs.
6. method according to claim 1, it is characterised in that the frequency discrimination of the Terahertz frequency domain spectra after being fourier transformed
Rate is 25.5GHz, and spectral range is 0.1~3.5THz.
7. method according to claim 1, it is characterised in that extract Terahertz optical transmission spectra, refractive index spectra, absorb system
After number spectrum, extinction coefficient spectrum, chosen spectrum scope carries out offset minimum binary linear discriminant analysis for 0.3~2THz spectral coverage
(PLS-LDA) model.
8. method according to claim 1, it is characterised in that the method smoothly pre-processed is flat for Savitzky-Golay
It is sliding.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108458989A (en) * | 2018-04-28 | 2018-08-28 | 江苏建筑职业技术学院 | A kind of Coal-rock identification method based on Terahertz multi-parameter spectrum |
| CN108458989B (en) * | 2018-04-28 | 2020-10-09 | 江苏建筑职业技术学院 | Terahertz multi-parameter spectrum-based coal rock identification method |
| CN109187422A (en) * | 2018-11-07 | 2019-01-11 | 浙江大学 | The recognition methods by diaphania harm initial stage mulberry leaf based on terahertz light spectral technology |
| CN110108647A (en) * | 2019-04-30 | 2019-08-09 | 深圳市太赫兹科技创新研究院有限公司 | A kind of discrimination method and identification system of meat kind |
| CN111351766A (en) * | 2020-02-27 | 2020-06-30 | 浙江大学 | Method for rapidly identifying identity of pumpkin seeds |
| CN112666119A (en) * | 2020-12-03 | 2021-04-16 | 山东省科学院自动化研究所 | Method and system for detecting ginseng tract geology based on terahertz time-domain spectroscopy |
| CN113390819A (en) * | 2021-06-11 | 2021-09-14 | 西南科技大学 | Terahertz sensor |
| CN113390819B (en) * | 2021-06-11 | 2022-12-06 | 西南科技大学 | Terahertz sensor |
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