CN107325993A - High-efficient culture chicken rhinitis haemophilus paragallinarum culture medium and its preparation method and application - Google Patents
High-efficient culture chicken rhinitis haemophilus paragallinarum culture medium and its preparation method and application Download PDFInfo
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Abstract
The present invention relates to a kind of high-efficient culture chicken rhinitis haemophilus paragallinarum culture medium and its preparation method and application, culture medium prescription is scientific and reasonable, serum is replaced with chick embryo allantoic liquid, cost can be substantially reduced, and be conducive to cultivating the growth of chicken rhinitis haemophilus paragallinarum culture medium, and compared with other commercially available culture mediums, not only cost reduction by 20% 30%, and the chicken rhinitis haemophilus paragallinarum thalline number grown improves 5% 500%, can be widely applied for the culture of chicken rhinitis haemophilus paragallinarum.
Description
Technical field
The invention belongs to culture medium field, and in particular to high-efficient culture chicken rhinitis haemophilus paragallinarum culture medium, further relate to
The preparation method and application of culture medium.
Background technology
Haemophilus paragallinarum is gram-Negative bacillus, is one of important conditioned pathogen and hospital-acquired infection bacterium.By
Its caused chicken rhinitis haemophilus paragallinarum disease is to endanger a kind of contagious infection of the poultry such as chicken, turkey and pheasant and wild fowl
Disease.The sick morbidity and mortality are generally 10~30%, but the diseased chicken for the chicken house infected chicken rhinitis haemophilus paragallinarum having is dead
The rate of dying is up to 75%.Once the chicken mass-sending live chickens rhinitis haemophilus paragallinarum infection in somewhere, the disease will turn into region disease
Disease, it is difficult to thoroughly eradicate, and often repeated explosion, bring huge economic loss.Chicken rhinitis haemophilus paragallinarum infection with
It is major pathologic features to cause atrophic rhinitis, foul smelling, and septicemia, urinary infection etc., on pathology very
It is difficult to be distinguished with other bacillary rhinitis infection.So in order to prevent and treat haemophilus paragallinarum infection, it is necessary to the bloodthirsty bar of secondary chicken
Bacterium is cultivated.But existing culture chicken rhinitis haemophilus paragallinarum culture medium, its composition includes expensive and bad system
Standby sterile chicken serum, its preparation method is complex and high cost.Therefore, it is badly in need of a kind of culture effect good, the low culture chicken of cost
Rhinitis haemophilus paragallinarum culture medium.
The content of the invention
In view of this, an object of the present invention is to provide a kind of high-efficient culture chicken rhinitis haemophilus paragallinarum culture
Base, scientific formulation rationally, serum is replaced with chick embryo allantoic liquid, is conducive to cultivating the life of chicken rhinitis haemophilus paragallinarum culture medium
It is long；The second object of the present invention is the preparation method for providing high-efficient culture chicken rhinitis haemophilus paragallinarum culture medium；The present invention
The third purpose be provide high-efficient culture chicken rhinitis haemophilus paragallinarum culture medium application.
To reach above-mentioned purpose, the present invention provides following technical scheme：
1st, high-efficient culture chicken rhinitis haemophilus paragallinarum culture medium, including following component：Sterile chick embryo allantoic liquid 0.1-
2.0g/L, yeast extract 0.1-2.0g/L, casein hydrolysate 0.1-2.0g/L, solvent are water, and pH value is 7.1~7.5.
It is preferred that, including following component：Sterile chick embryo allantoic liquid 0.2g/L, yeast extract 0.6g/L, casein hydrolysate
0.6g/L, solvent is water, and pH value is 7.1.
It is preferred that, including following component：Sterile chick embryo allantoic liquid 0.1g/L, yeast extract 0.5g/L, casein hydrolysate
0.5g/L, solvent is water, and pH value is 7.2.
It is preferred that, including following component：Sterile chick embryo allantoic liquid 1g/L, yeast extract 1g/L, casein hydrolysate 0.8g/
L, solvent is water, and pH value is 7.3.
It is preferred that, including following component：Sterile chick embryo allantoic liquid 0.8g/L, yeast extract 0.9g/L, casein hydrolysate
0.9g/L, solvent is water, and pH value is 7.5.
2. described in high-efficient culture chicken rhinitis haemophilus paragallinarum culture medium preparation method, comprise the following steps, by proportioning
Yeast extract and casein hydrolysate are taken, is then dissolved in the water of proportional quantity, is mixed, 121 DEG C of autoclavings 20-30 minutes are treated
When Temperature fall is to less than 30 DEG C, sterile chick embryo allantoic liquid, regulation pH value to 7.1~7.5 are proportionally added under aseptic condition.
3. described in high-efficient culture chicken rhinitis haemophilus paragallinarum culture medium in high-efficient culture chicken rhinitis haemophilus paragallinarum
Application.
The beneficial effects of the present invention are：The invention discloses high-efficient culture chicken rhinitis parachicken blood phili bacillus liquid culture medium
And its preparation method and application, the culture medium can guarantee that chicken rhinitis haemophilus paragallinarum under conditions of growth, can well drop significantly
Low cost, it is simpler, more scientific reasonable to be formulated, and is that high-efficient culture chicken rhinitis haemophilus paragallinarum creates suitable growth ring
Border, compared with other commercially available culture mediums, not only cost reduction 20%-30%, and the chicken rhinitis haemophilus paragallinarum grown
Thalline number improves 5%-500%, can be widely applied for the culture of chicken rhinitis haemophilus paragallinarum.
Brief description of the drawings
In order that the purpose of the present invention, technical scheme and beneficial effect are clearer, the present invention provides drawings described below and carried out
Explanation：
Fig. 1 is that embodiment 5 cultivates chicken rhinitis haemophilus paragallinarum count results figure.
Fig. 2 is that comparative example 1 cultivates chicken rhinitis haemophilus paragallinarum count results figure.
Fig. 3 is that embodiment 6 cultivates chicken rhinitis haemophilus paragallinarum count results figure.
Fig. 4 is that comparative example 1 cultivates chicken rhinitis haemophilus paragallinarum count results figure.
Embodiment
Below in conjunction with accompanying drawing, the preferred embodiments of the present invention are described in detail.It is unreceipted specific in embodiment
The experimental method of condition, generally according to normal condition or according to the condition proposed by manufacturer.
Embodiment 1
High-efficient culture chicken rhinitis parachicken blood phili bacillus liquid culture medium, its formula is as follows：Sterile chick embryo allantoic liquid 0.2g/L,
Yeast extract 0.6g/L, casein hydrolysate 0.6g/L, solvent is water.
The preparation method of high-efficient culture chicken rhinitis parachicken blood phili bacillus liquid culture medium, takes 0.6g yeast extracts and 0.6g junket
Protolysate is dissolved in 1L distilled water, is mixed, and 121 DEG C of autoclavings 20-30 minutes treat Temperature fall to less than 30 DEG C
When, the sterile sterile chick embryo allantoic liquid 0.2g of addition, regulation pH value is 7.1.
Embodiment 2
High-efficient culture chicken rhinitis parachicken blood phili bacillus liquid culture medium, its formula is as follows：Sterile chick embryo allantoic liquid 0.1g/L,
Yeast extract 0.5g/L, casein hydrolysate 0.5g/L, solvent is water.
The preparation method of high-efficient culture chicken rhinitis parachicken blood phili bacillus liquid culture medium, takes 0.5g yeast extracts and 0.5g junket
Protolysate is dissolved in 1L distilled water, is mixed, and 121 DEG C of autoclavings 20-30 minutes treat Temperature fall to less than 30 DEG C
When, the sterile sterile chick embryo allantoic liquid 0.1g of addition, regulation pH value is 7.2.
Embodiment 3
High-efficient culture chicken rhinitis parachicken blood phili bacillus liquid culture medium, its formula is as follows：Sterile chick embryo allantoic liquid 1g/L, ferment
Mother leaching powder 1g/L, casein hydrolysate 0.8g/L, solvent is water.
The preparation method of high-efficient culture chicken rhinitis parachicken blood phili bacillus liquid culture medium, takes 1g yeast extracts and 0.8g junket eggs
White hydrolysate is dissolved in 1L distilled water, is mixed, 121 DEG C of autoclavings 20-30 minutes, when Temperature fall is to less than 30 DEG C,
Sterile to add sterile chick embryo allantoic liquid 1g, regulation pH value is 7.3.
Embodiment 4
High-efficient culture chicken rhinitis parachicken blood phili bacillus liquid culture medium, its formula is as follows：Sterile chick embryo allantoic liquid 0.8g/L,
Yeast extract 0.9g/L, casein hydrolysate 0.9g/L, solvent is water.
The preparation method of high-efficient culture chicken rhinitis parachicken blood phili bacillus liquid culture medium, takes 0.9g yeast extracts and 0.9g junket
Protolysate is dissolved in 1L distilled water, is mixed, and 121 DEG C of autoclavings 20-30 minutes treat Temperature fall to less than 30 DEG C
When, the sterile sterile chick embryo allantoic liquid 0.8g of addition, regulation pH value is 7.5.
Embodiment 5
It is bloodthirsty using the obtained secondary chicken of high-efficient culture chicken rhinitis haemophilus paragallinarum medium culture chicken rhinitis in embodiment 2
Bacillus, comprises the following steps that：
Take chicken rhinitis haemophilus paragallinarum 1mL liquid inoculations bloodthirsty in the secondary chicken of high-efficient culture chicken rhinitis that embodiment 2 is obtained
In bacillus fluid nutrient medium, agar plate culture is counted after being cultivated 3 days under the conditions of 30-37 DEG C, as a result as shown in Figure 1.As a result
It has been shown that, chicken rhinitis haemophilus paragallinarum is quickly bred in culture medium made from embodiment 2.
Comparative example 1
Using commercially available chicken rhinitis parachicken blood phili bacillus liquid culture medium, each component and preparation method are as follows：
Beef leachate 1000ml, peptone 10g, sodium chloride 5g and aseptic de-fiber sheep blood or rabbit blood 60ml.
According to the method culture chicken rhinitis haemophilus paragallinarum of embodiment 5, as a result as shown in Figure 2.
As a result show, the bacterium colony grown using the obtained culture chicken rhinitis parachicken blood phili bacillus liquid culture medium of embodiment 2
Number is high by about 50% higher than commercially available culture medium, shows the culture chicken rhinitis parachicken blood phili bacillus liquid culture medium that the present invention is obtained
Be conducive to cultivating the growth of chicken rhinitis haemophilus paragallinarum, and cost is lower, low 20%-30%.
Embodiment 6
It is bloodthirsty using the obtained secondary chicken of culture chicken rhinitis parachicken blood phili bacillus liquid culture medium culture chicken rhinitis in embodiment 1
Bacillus, the present embodiment uses solid medium, and specific method is as follows：
Chicken rhinitis haemophilus paragallinarum is taken to be inoculated in culture chicken rhinitis parachicken blood phili bacillus liquid culture medium, at 30-37 DEG C
Under the conditions of culture 3 days after agar plate culture count, carry out four ride cultures, 20-30 DEG C cultivate 2 days, as a result such as Fig. 3 institutes
Show.As a result show, chicken rhinitis haemophilus paragallinarum is growing a large amount of bacterium colonies on culture medium made from embodiment 1, show the present invention
Obtained culture medium is adapted to the growth of chicken rhinitis haemophilus paragallinarum.
Comparative example 2
Using commercially available chicken rhinitis parachicken blood phili bacillus liquid culture medium, each component and concentration are as follows：Beef leachate
1000ml, peptone 10g, sodium chloride 5g, aseptic de-fiber sheep blood or rabbit blood 60ml.
Counted according to the method culture chicken rhinitis haemophilus paragallinarum of embodiment 5, as a result as shown in Figure 4.
As a result show, the clump count grown using the obtained culture chicken rhinitis haemophilus paragallinarum culture medium of embodiment 1 is high
It is high by about 10% in commercially available culture medium, show that the culture chicken rhinitis haemophilus paragallinarum culture medium that the present invention is obtained is conducive to chicken nose
The growth of scorching haemophilus paragallinarum culture medium, and cost is lower, low 20%-30%.
The obtained culture chicken rhinitis haemophilus paragallinarum culture medium of embodiment 3 and 4 is similar to Examples 1 and 2, clump count difference
Higher than the 8% of commercially available culture medium and 12%.
In the present invention, sterile chick embryo allantoic liquid 0.1-2.0g/L, yeast extract 0.1-2.0g/L, casein hydrolysate 0.1-
2.0g/L, solvent is water, and pH value is 7.1~7.5, and goal of the invention can be achieved.
It follows that culture chicken rhinitis haemophilus paragallinarum culture medium produced by the present invention is the training of a high efficiency, low cost
Base is supported, is more beneficial for cultivating the growth of chicken rhinitis haemophilus paragallinarum culture medium.
Finally illustrate, preferred embodiment above is merely illustrative of the technical solution of the present invention and unrestricted, although logical
Cross above preferred embodiment the present invention is described in detail, it is to be understood by those skilled in the art that can be
Various changes are made to it in form and in details, without departing from claims of the present invention limited range.
Claims (7)
1. high-efficient culture chicken rhinitis haemophilus paragallinarum culture medium, it is characterised in that including following component：Sterile chick embryo allantoic liquid
0.1-2.0g/L, yeast extract 0.1-2.0g/L, casein hydrolysate 0.1-2.0g/L, solvent are water, and pH value is 7.1 ~ 7.5.
2. high-efficient culture chicken rhinitis haemophilus paragallinarum culture medium according to claim 1, it is characterised in that including such as the following group
Point：Sterile chick embryo allantoic liquid 0.2g/L, yeast extract 0.6g/L, casein hydrolysate 0.6g/L, solvent is water, and pH value is
7.1。
3. high-efficient culture chicken rhinitis haemophilus paragallinarum culture medium according to claim 1, it is characterised in that including such as the following group
Point：Sterile chick embryo allantoic liquid 0.1g/L, yeast extract 0.5g/L, casein hydrolysate 0.5g/L, solvent are water, and pH value is 7.2.
4. high-efficient culture chicken rhinitis haemophilus paragallinarum culture medium according to claim 1, it is characterised in that including such as the following group
Point：Sterile chick embryo allantoic liquid 1g/L, the g/L of yeast extract 1, casein hydrolysate 0.8g/L, solvent is water, and pH value is 7.3.
5. high-efficient culture chicken rhinitis haemophilus paragallinarum culture medium according to claim 1, it is characterised in that including such as the following group
Point：Sterile chick embryo allantoic liquid 0.8g/L, the g/L of yeast extract 0.9, casein hydrolysate 0.9g/L, solvent is water, and pH value is
7.5。
6. the preparation method of any one of the claim 1-5 high-efficient culture chicken rhinitis haemophilus paragallinarum culture mediums, its feature
It is：Comprise the following steps, yeast extract and casein hydrolysate taken by proportioning, be then dissolved in the water of proportional quantity, mix,
121 DEG C of autoclavings 20-30 minutes, when Temperature fall is to less than 30 DEG C, are proportionally added into sterile chicken embryo urine under aseptic condition
Cyst fluid, regulation pH value to 7.1 ~ 7.5.
7. any one of the claim 1-5 high-efficient culture chicken rhinitis haemophilus paragallinarum culture mediums are in high-efficient culture chicken rhinitis pair
Application in haemophilus gallinarum.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108815517A (en) * | 2018-07-13 | 2018-11-16 | 广东永顺生物制药股份有限公司 | A kind of Duck plague live vaccine and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1297041A (en) * | 1999-11-23 | 2001-05-30 | 山东省农业科学院家禽研究所 | Culture medium prepared from chicken embryo for bacteria (e.g. infections chicken rhinitis bacterium) |
| CN104099273A (en) * | 2014-07-11 | 2014-10-15 | 重庆市畜牧科学院 | Culture medium for specifically separating intestinal bacteria capable of decomposing TMAO (tirmethylamine N-oxide) as well as preparation method and application thereof |
| CN106190909A (en) * | 2016-07-21 | 2016-12-07 | 山东滨州沃华生物工程有限公司 | Haemophilus paragallinarum Type B strain fermentation medium, its preparation method and application thereof |
-
2017
- 2017-08-31 CN CN201710773987.4A patent/CN107325993A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1297041A (en) * | 1999-11-23 | 2001-05-30 | 山东省农业科学院家禽研究所 | Culture medium prepared from chicken embryo for bacteria (e.g. infections chicken rhinitis bacterium) |
| CN104099273A (en) * | 2014-07-11 | 2014-10-15 | 重庆市畜牧科学院 | Culture medium for specifically separating intestinal bacteria capable of decomposing TMAO (tirmethylamine N-oxide) as well as preparation method and application thereof |
| CN106190909A (en) * | 2016-07-21 | 2016-12-07 | 山东滨州沃华生物工程有限公司 | Haemophilus paragallinarum Type B strain fermentation medium, its preparation method and application thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108815517A (en) * | 2018-07-13 | 2018-11-16 | 广东永顺生物制药股份有限公司 | A kind of Duck plague live vaccine and preparation method thereof |
| CN108815517B (en) * | 2018-07-13 | 2021-09-14 | 广东永顺生物制药股份有限公司 | Duck plague live vaccine and preparation method thereof |
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