CN107312619A - It is a kind of to improve the method that walnut kernel oil body extracts integrity degree - Google Patents

It is a kind of to improve the method that walnut kernel oil body extracts integrity degree Download PDF

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Publication number
CN107312619A
CN107312619A CN201710644445.7A CN201710644445A CN107312619A CN 107312619 A CN107312619 A CN 107312619A CN 201710644445 A CN201710644445 A CN 201710644445A CN 107312619 A CN107312619 A CN 107312619A
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China
Prior art keywords
walnut kernel
oil body
walnut
integrity degree
kernel
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CN201710644445.7A
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Chinese (zh)
Inventor
张普娟
刘广勤
朱海军
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Jiangsu Academy of Agricultural Sciences
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Jiangsu Academy of Agricultural Sciences
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Priority to CN201710644445.7A priority Critical patent/CN107312619A/en
Publication of CN107312619A publication Critical patent/CN107312619A/en
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    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11BPRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00Production of fats or fatty oils from raw materials
    • C11B1/02Pretreatment
    • C11B1/04Pretreatment of vegetable raw material
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11BPRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00Production of fats or fatty oils from raw materials
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11BPRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00Refining fats or fatty oils
    • C11B3/001Refining fats or fatty oils by a combination of two or more of the means hereafter
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11BPRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00Refining fats or fatty oils
    • C11B3/008Refining fats or fatty oils by filtration, e.g. including ultra filtration, dialysis
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11BPRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00Refining fats or fatty oils
    • C11B3/16Refining fats or fatty oils by mechanical means

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Mechanical Engineering (AREA)
  • Fats And Perfumes (AREA)

Abstract

The method that walnut kernel oil body extracts integrity degree is improved the invention discloses a kind of, its extraction process includes the broken shell to walnut, the immersion of walnut kernel, grinding, the centrifugation of walnut pulp solution, the flows such as separation, wherein broken shell will avoid the mechanical damage to walnut kernel, the walnut kernel of collection is soaked using deionized water, then walnut kernel is added in the grinding beater of the buffer solution containing Tris HCl, carry out mashing filtration treatment, centrifugal treating finally is carried out to walnut kernel slurries using centrifuge, walnut kernel oil body is collected after standing, complete the extraction process of whole walnut kernel oil body.The oil body of extraction is observed under an optical microscope, this method can effectively improve the integrity degree of walnut kernel oil body extraction, there is facilitation to the quality for improving walnut oil.

Description

It is a kind of to improve the method that walnut kernel oil body extracts integrity degree
Technical field
The method that walnut kernel oil body extracts integrity degree is improved the present invention relates to a kind of, belongs to vegetable fat process technology neck Domain.
Background technology
Many seeds, dry fruit contain higher grease, and these greases are mainly housed in small volume, independent sphaerocyst device In oil body.Oil body is also referred to as liposome, elaiosome, as energy supply station, is mainly used to the metabolic activity of sertoli cell.Oil body by Middle cardiolipin, mainly triglycerides (TAG), and be inlaid with the phosphoric acid membrane bound of protein and form.Protein and phosphoric acid Together, by surface charge and space steric effect, to stablize oil body structure, protection oil body turns into independent completion to film Individual.Intact oil bodies unrighted acid, oxidation preventive content are higher, and with non-Aggregation Characteristics, therefore in food, biological skill There is very extensive purposes in terms of art, pharmacy application.
The extracting method of conventional walnut kernel oil body mainly includes 3 steps:(1) cell tissue comprising oil body is beaten It is broken, in order to which oil body is discharged into the aqueous solution;(2) the tissue impurity in addition to oil body is filtered out, and is obtained comprising oil body Milky is homogenized;(3) centrifugal treating is carried out to milky homogenate, collects the automatic oil body for floating to liquid surface.To including oil body Cell tissue is broken, homogenate centrifugation is the committed step for obtaining intact oil bodies, while needed for different tissues, different samples Parameter it is different.Up to now, the parameter and method that the higher walnut oil body of oil content is extracted also are not very clear and definite.
The content of the invention
In order to improve the integrity degree of walnut kernel oil body extraction, the oil body morphology intracellular to walnut kernel under micro and Biochemical characteristic is studied, and finally invents a kind of method that can improve walnut kernel oil body extraction integrity degree.
The key technology of the present invention is to be ground walnut kernel before mashing flow, increase immersion link, and to grinding It is beaten walnut kernel link and adds a certain amount of buffer reagent, and starch walnut kernel the scope of the centrifugal force of solution and is configured.Carry The method detailed step that high walnut kernel oil body extracts integrity degree is as follows:
Skin breaking is carried out to walnut first, the protection to walnut kernel is noted during broken shell, it is to avoid walnut kernel is made Screening removing is carried out into mechanical failure, and to mechanically damaged walnut kernel;
Walnut kernel after broken shell is weighed, then the walnut kernel after weighing is placed in clean container, to container The deionized water of 5~10 times of walnut kernel quality of interior addition, under 4 DEG C of environment, soaks to walnut kernel, soak time 6~ In 24 hours;
After the completion of walnut kernel immersion, the walnut kernel after immersion is filtered, beater or grinder is put into, and add 5~10 The buffer reagent of times walnut kernel quality, buffer reagent is 0.1M Tris-HCl, and pH is 8.0, wherein the EDTA comprising 0.1mM;
The walnut kernel for adding buffer reagent is carried out to crush mashing processing using beater or grinder, mashing is crushed and finishes Afterwards, impurity is filtered out using filter, obtains the milky comprising oil body and be homogenized;
Homogenate after filtering is placed in centrifuge, under 4 DEG C of environment temperature, using centrifuge to including oil body Milky homogenate carries out centrifugal treating, sets the centrifugal force of centrifuge in 3000RCF~7500RCF scopes, runs 20 minutes;
Walnut kernel homogenate after centrifugation is stood, the automatic oil body for floating to liquid surface is collected, completes whole oil body Lift process.
In order to examine the actual techniques effect of this method, contrasted with conventional walnut kernel oil body extraction process, respectively The oil body sample 0.1g that two methods are obtained is taken, is added in 0.5ml deionized waters, after being well mixed, optical microphotograph is placed on Under mirror, the integrity degree of oil body size in two portions of mixed liquors of observation, and with the size of grain size measurement device measure oil body, to detect The integrity degree of walnut oil body.
By contrast two samples under an optical microscope it can be found that the oil body granularity extracted using the inventive method compared with Small, more uniform, the result of grain size measurement device also illustrates the oil body obtained after walnut kernel immersion, with the intracellular oil body of walnut kernel Size is consistent substantially, and above comparing result can illustrate that the oil body extracting method after improving is carried to improving walnut kernel oil body Take integrity degree that there is facilitation.
The present invention to conventional walnut oil body extracting method by optimizing, it is possible to reduce mechanical lapping is thin to walnut kernel The extent of injury of born of the same parents, improves the integrity degree of walnut kernel oil body, and then can reach the technique effect for improving walnut oil quality.
Brief description of the drawings
Fig. 1 is the schematic flow sheet of the inventive method
Fig. 2 is electromicroscopic photograph of the distilled water as oil body during buffer solution
Fig. 3 is electromicroscopic photographs of the Tris-HCl as oil body during buffer solution
Fig. 4 is the electromicroscopic photograph of oil body when centrifugal force is 5500RCF
Fig. 5 is the electromicroscopic photograph of oil body when centrifugal force is 10000RCF
Fig. 6 is walnut kernel without the oil body electromicroscopic photograph extracted after immersion
Fig. 7 is the electromicroscopic photograph for the oil body that walnut kernel is extracted after immersion
Embodiment
Technical scheme, implementation process etc. are done with reference to specific embodiments and the drawings and further explained in detail State.
Embodiment 1
A kind of to improve the method that walnut kernel oil body extracts integrity degree as shown in figure (1), step (1) is broken to walnut Shell processing, notes the protection to walnut kernel during broken shell, it is to avoid cause scratch to damage to walnut kernel, and walnut kernel has been received Screening removing is carried out to abrasive walnut kernel;
Step (2), is weighed to the walnut kernel after broken shell, and the walnut kernel after weighing then is placed on into clean container Interior, the deionized water to adding 5~10 times of walnut kernel quality in container under 4 DEG C of environment, is soaked to walnut kernel, soaked Time is kept for 6 hours;
Step (3), after the completion of walnut kernel immersion, the walnut kernel after immersion is filtered out, beater or grinding is put into Machine, and the buffer reagent of 5~10 times of walnut kernel quality is added, buffer reagent is 0.1M Tris-HCl, and pH is 8.0, comprising 0.1mM EDTA;
Step (4), carries out crushing mashing processing, powder using beater or grinder to the walnut kernel for adding buffer reagent After broken mashing is finished, impurity is filtered out using filter, and obtain the milky homogenate comprising oil body;
Step (5), the homogenate after filtering is placed in centrifuge, under 4 DEG C of environment temperature, uses centrifuge pair Milky homogenate comprising oil body carries out centrifugal treating, and the centrifugal force for setting centrifuge is 3000RCF, is run 20 minutes;
Step (6), the walnut kernel homogenate after centrifugation is stood, and is collected the automatic oil body for floating to liquid surface, is completed whole Individual oil body lifts process.
Embodiment 2
The step of the present embodiment (one), step (3), step (4), step (5) and step (6) are same as Example 1, The parameter setting of wherein step (2) is different;
Step (2), the walnut kernel of collection is weighed, and then the walnut kernel after weighing is placed in clean container, And to adding the deionized water of 5~10 times of walnut kernel quality in container, under 4 DEG C of environment, walnut kernel is soaked, during immersion Between keep 12 hours;
Embodiment 3
The step of the present embodiment (one), step (3), step (4), step (5) and step (6) are same as Example 1, The parameter setting of wherein step (2) is different;
Wherein step (2), the walnut kernel of collection is weighed, and the walnut kernel after weighing then is placed on into clean appearance In device, and to adding the deionized water of 5~10 times of walnut kernel quality in container, under 4 DEG C of environment, walnut kernel is soaked, Soak time is kept for 24 hours;
Embodiment 4
The step of the present embodiment (one), step (2), step (3), step (4) and step (6) are same as Example 3, The parameter setting of wherein step (5) is different;
Wherein step (5), the homogenate after filtering is placed in centrifuge, under 4 DEG C of environment temperature, uses centrifugation Machine carries out centrifugal treating to walnut kernel slurry solution, and the centrifugal force for setting centrifuge is 5500RCF, is run 20 minutes.
Embodiment 5
The step of the present embodiment (one), step (2), step (3), step (4) and step (6) are same as Example 3, The parameter setting of wherein step (5) is different;
Wherein step (5), the solution after filtering is placed in centrifuge, under 4 DEG C of environment temperature, uses centrifugation Machine carries out centrifugal treating to the milky homogenate comprising oil body, and the centrifugal force for setting centrifuge is 7500RCF, is run 20 minutes.
Embodiment 6
The step of the present embodiment (one), step (3), step (4), step (5) and step (6) are same as Example 4, The parameter setting of wherein step (2) is different;
Step (2), the walnut kernel of collection is weighed, and then the walnut kernel after weighing is placed in clean container, And to adding the deionized water of 10 times of walnut kernel quality in container, under 4 DEG C of environment, walnut kernel is soaked, soak time Kept for 12 hours.
Embodiment 7
The step of the present embodiment (one), step (3), step (4), step (5) and step (6) are same as Example 4, The parameter setting of wherein step (2) is different;
Step (2), the walnut kernel of collection is weighed, and then the walnut kernel after weighing is placed in clean container, And to adding the deionized water of 10 times of walnut kernel quality in container, under 4 DEG C of environment, walnut kernel is soaked, soak time Kept for 24 hours.
7 embodiments of the above are all the oil body samples produced according to embodiments of the invention by verification experimental verification, are taken wherein Sample 0.1g, is added in 0.5ml deionized waters, be well mixed after, placement observe under an optical microscope, and with conventional core Peach kernel oil body extracting method is analyzed, and analysis result is as follows:
Fig. 2 is the electromicroscopic photograph that oil body is extracted when distilled water is buffer solution, and Fig. 3 is when using Tris-HCl as buffer solution The electromicroscopic photograph of oil body is extracted, under an optical microscope by contrast, walnut kernel is ground using Tris-HCl as buffer solution Mill, can improve the integrity degree for extracting oil body.
Fig. 4 is the electromicroscopic photograph that oil body is extracted when the centrifugal force of centrifuge is 5500RCF, and Fig. 5 is the centrifugal force of centrifuge The electromicroscopic photograph of oil body is extracted during for 10000RCF, under an optical microscope by contrast, the centrifugal force of centrifuge is 5500RCF When, the integrity degree for extracting oil body can be improved.
Fig. 6 is electromicroscopic photograph of the walnut kernel without extraction oil body during immersion, and Fig. 7 is extracted when being and being soaked using deionized water The electromicroscopic photograph of oil body, under an optical microscope by contrast, is soaked to walnut kernel using deionized water, can improve and carry Take the integrity degree of oil body.
Specific embodiment above and comparative analysis result, it can be deduced that the oil body extracting method of the application present invention are right The integrity degree tool of walnut kernel oil body is greatly improved, and then can lift the quality for squeezing walnut oil.

Claims (4)

1. a kind of improve the method that walnut kernel oil body extracts integrity degree, it is characterised in that:Walnut kernel slurries are fabricated in walnut kernel Before, increase immersion link, and a certain amount of buffer reagent is added to grinding mashing link, starch solution centrifugal link for walnut kernel Centrifugal force scope be configured, comprise the following steps that:
(1) the raw material walnut for preparing walnut oil is subjected to skin breaking, notes the protection to walnut kernel in broken shell process, it is to avoid right Walnut kernel causes mechanical damage, and the walnut kernel of damage is carried out into screening removing in time;
(2) walnut kernel after broken shell is weighed, then the walnut kernel after weighing is placed in clean container, in container The soaking solution of 5~10 times of walnut kernel quality is added, under 4 DEG C of environment, walnut kernel is soaked;
(3) the walnut kernel filtering completed will be soaked, be placed in beater or grinder, and add 5~10 times of walnut kernel quality Buffer reagent;
(4) walnut kernel for adding buffer reagent is carried out to crush mashing processing using beater or grinder, after crushing is finished, made Impurity is filtered out with filter, the milky comprising oil body is obtained and is homogenized;
(5) milky homogenate is placed in centrifuge, in the environment of 4 DEG C, carries out centrifugal treating;
(6) milky after centrifugation is homogenized and stood, collect the automatic oil body for floating to liquid surface, completed whole oil body and extracted Journey.
2. a kind of method for improving walnut kernel oil body extraction integrity degree according to claim 1, it is characterised in that described Soaking solution is deionized water.
3. a kind of method for improving walnut kernel oil body extraction integrity degree according to claim 1, it is characterised in that described Buffer reagent is 0.1M Tris-HCl, and pH is 8.0, wherein the EDTA comprising 0.1mM.
A kind of improve the method that walnut kernel oil body extracts integrity degree 4. according to claim 1, it is characterised in that it is described from The centrifugal force controlled of heart processing is run 20 minutes in the range of 3000RCF~7500RCF.
CN201710644445.7A 2017-07-25 2017-07-25 It is a kind of to improve the method that walnut kernel oil body extracts integrity degree Pending CN107312619A (en)

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Cited By (2)

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Publication number Priority date Publication date Assignee Title
CN110463771A (en) * 2019-09-18 2019-11-19 江南大学 A kind of preparation method of the Walnut protein powder of high protein content
CN114032141A (en) * 2021-11-17 2022-02-11 八叶草健康产业研究院(厦门)有限公司 Peony seed oil extracting solution and extracting method thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110463771A (en) * 2019-09-18 2019-11-19 江南大学 A kind of preparation method of the Walnut protein powder of high protein content
CN114032141A (en) * 2021-11-17 2022-02-11 八叶草健康产业研究院(厦门)有限公司 Peony seed oil extracting solution and extracting method thereof

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Application publication date: 20171103