CN107308924A - A kind of polyether-ether-ketone capillary electric chromatographic column of poly-dopamine modification and its application - Google Patents
A kind of polyether-ether-ketone capillary electric chromatographic column of poly-dopamine modification and its application Download PDFInfo
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- CN107308924A CN107308924A CN201710438672.4A CN201710438672A CN107308924A CN 107308924 A CN107308924 A CN 107308924A CN 201710438672 A CN201710438672 A CN 201710438672A CN 107308924 A CN107308924 A CN 107308924A
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- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
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Abstract
The invention discloses a kind of polyether-ether-ketone capillary electric chromatographic column of poly-dopamine modification and its application.Its preparation method is as follows：The dopamine solution of pre-oxidation is passed through in polyether-ether-ketone pipe, the surface not only amino with positively charged but also the poly-dopamine coating with electronegative phenolic hydroxyl group is formed, to realize the adjustability of EOF.Modification of the poly-dopamine to inertia polyether-ether-ketone pipe can strengthen the adjustability of its EOF, expand its application in capillary electric chromatogram.Preparation method of the present invention is simple, and the coating uniform of formation is fine and close, and thickness is controllable, without obscission after long-time use.Polyether-ether-ketone capillary port stability and durability are good, Swelling will not occur when being connected with mass spectrum, it is adaptable to several amino acids, the separation and detection of aldehydes matter and plant hormone.
Description
Technical field
The invention belongs to chromatographic technology field, and in particular to a kind of electric color of polyether-ether-ketone capillary of poly-dopamine modification
Compose post and preparation method and application.
Background technology
Capillary electrophoresis-laser-induced fluorescence technology is one grown up on the basis of LC-MS-MS
New analytical technology, it is combined, and capillary electrophoresis separation efficiency high, analyze speed be fast, sample consumption is few and Mass Spectrometer Method
Utensil has the advantages that both high sensitivity and strong structure analytic ability, has become the novel micro for enjoying analytical chemist to pay close attention to
Analytical technology.At present, the Fused-silica capillary column of pi parcel with good toughness, optics permeability, heat because pass
It is current the features such as leading the silicone hydroxyl of ability and inside stable EOF can be provided and be conducive to the fixation of chromatographic material
Most-often used piping material in Capillary Electrophoresis.But it has pi coating in organic solvent and alkaline solution
Unstable the problem of, particularly in capillary electrophoresis-laser-induced fluorescence, high temperature, high voltage, the organic solvent of mass spectrum spraying port
Under conditions of content is high, the capillary is easily swelled to be acted on aminolysis, and so as to cause the blocking of port, spraying is not
Stable a series of problems, such as, impact analysis result sets infringement equipment.( Yamamoto, M.; Ly, R.; Gill, B.;
Zhu, Y.; Moran-Mirabal, J.; Britz-McKibbin, P. Anal. Chem. 2016, 88, 10710-
10719)。
Polyetheretherketonematerials materials are because heat endurance is good, and chemical stability is strong, is commonly used for high performance liquid chromatography, gas-chromatography
In pipeline Deng instrument, but because optics permeability is poor, EOF ability of regulation and control difference and chemical inertness are unfavorable for by force chromatogram
The shortcomings of modification of material, it is rarely used at present in Capillary Electrophoresis.
The content of the invention
To overcome problems of the prior art, it is adjustable that the present invention provides a kind of stable EOF, direction and size
The polyether-ether-ketone capillary electric chromatographic column of poly-dopamine modification, capillary electric chromatogram-mass spectrometry separation for many kinds of substance
Analysis.
Concrete technical scheme is as follows：
A kind of polyether-ether-ketone capillary electric chromatographic column of poly-dopamine modification, is prepared by the following method and obtains：
1）1 ~ 2 mg/mL dopamine alkaline solution is prepared, the pH of the solution is 8.5 ~ 9, logical oxygen makes its pre-oxidation to brown；
2）By step 1）Dopamine alkaline solution flowing after the pre-oxidation of gained passes through clean polyether-ether-ketone using peristaltic pump
Capillary column, the flow velocity of peristaltic pump is 0.01-0.1 mL/h, and the time of poly-dopamine modification is 1 ~ 24 hour, is placed in 60 DEG C of bakings
3 h of drying, are modified polyether-ether-ketone inside pipe wall in case.
The process of the poly-dopamine modification reaction of the present invention is as shown in Figure 1.The present invention is based on the bionical poly-dopamine of extra large freshwater mussel
Modification method.Dopamine can be in briny environment（Alkalescent）Under the conditions of occur polymerization ([2] Lee, H.; Dellatore, S.M.;
Miller, W.M.;Messersmith, P.B. Science 2007,318,426-430), in the polyethers being immersed
Ether ketone capillary inner surface forms one layer of stable structure with catechol and amino, and on the one hand the active group can be
Polyether-ether-ketone pipe provides direction and the adjustable EOF of size, can also solve the problem of its chemical inertness is stronger.
The polyether-ether-ketone capillary electric chromatographic column of described poly-dopamine modification is in capillary electrophoresis-laser-induced fluorescence analysis
Application.
The polyether-ether-ketone capillary electric chromatographic column of described poly-dopamine modification is analyzed in amino acid, phenols and plant hormone
Application in the capillary electrophoresis-laser-induced fluorescence separation analysis of thing.
The polyether-ether-ketone capillary electric chromatographic column of poly-dopamine modification in the present invention, has the following advantages that：
（1）The vitreous silica capillary of conventional pi parcel is replaced using polyether-ether-ketone pipe capillary is used for capillary electricity
Swimming-mass spectrometry analysis, can solve pi coating and easily be swelled in organic solvent and easy in the basic conditions
Occur the shortcoming of aminolysis, a stable port is provided for the GC-MS.
（2）Polyether-ether-ketone pipe surface is smooth and chemical inertness is high, the method coated using physics and common covalent modification
Method is difficult to chromatographic material being coated to its inner surface；The present invention will be poly- more with a large amount of amino and hydroxyl by poly-dopamine method
Bar amine layer modifies the inner surface in polyether-ether-ketone pipe, can effectively solve the problem of chemical inertness is strong.
（3）The modification of poly-dopamine layer, can provide stable for polyether-ether-ketone pipe, direction and the adjustable EOF of size,
It is conducive to separate the application in analyzing.Using the capillary after the modification, realize to several amino acids, phenols and plant
The good separating effect of hormone.
Brief description of the drawings
Fig. 1 is the process schematic of poly-dopamine modification reaction in polyether-ether-ketone pipe.
Fig. 2 is that the polyether-ether-ketone pipe that poly-dopamine is modified is used for the schematic diagram that CE-MS is separated.
Fig. 3 is the polyether-ether-ketone capillary electric chromatographic column of poly-dopamine modification prepared by embodiment 1 and unmodified and melting
The comparison that quartz capillary EOF changes with pH value.
Fig. 4 is the polyether-ether-ketone capillary electric chromatographic column of poly-dopamine modification prepared by embodiment 1 in positive EOF bar
To the separating effect figure of three Plant Hormones under part.
Fig. 5 is the polyether-ether-ketone capillary electric chromatographic column of poly-dopamine modification prepared by embodiment 1 in reverse EOF bar
To the separating effect figure of three kinds of amino acid under part.
Fig. 6 is the polyether-ether-ketone pipe capillary electric chromatogram of poly-dopamine modification prepared by embodiment 1 in positive EOF bar
To the separating effect figure of two kinds of aldehydes matters under part.
Embodiment
By combination accompanying drawing described further below it will be further appreciated that the features and advantages of the invention.The implementation provided
Example is only the explanation to the inventive method, without limiting remaining content that the present invention is disclosed in any way.
【Embodiment 1】
1）The mg of dopamine 10 is weighed, is dissolved in 10 mmol/L Tris-HCl buffer solutions, pH to 8.5 is adjusted, solution is shaken,
To solution in palm fibre.
2）With the mL/min of flow velocity 0.03 by step 1）The solution of gained passes through 50 clean cm（360 μm of external diameter, internal diameter
50 μm）Polyether-ether-ketone pipe, modifies 3 h at room temperature, and water washing, nitrogen blows away solvent, is placed in 60 DEG C of h of drying in oven 3, obtains
The polyether-ether-ketone capillary electric chromatographic column of poly-dopamine modification.
As shown in Fig. 2 the polyether-ether-ketone capillary electric chromatographic column that poly-dopamine made from embodiment 1 is modified is connected to capillary
Electrophoresis tube-mass spectrum（Using coaxial threeway sheath stream interface）In device, capillary electric chromatogram-mass spectrometry analysis, progressive is realized
It can test, it is as follows：
The polyether-ether-ketone capillary electric chromatographic column and unmodified and vitreous silica capillary of poly-dopamine modification prepared by embodiment 1
The result that pipe EOF changes with pH value is compared, and its result is as shown in figure 3, the polyether-ether-ketone hair modified without poly-dopamine
The EOF of forward direction is presented in tubule in the range of pH=3-9, all, and in pH=7-9, change of the EOF little with pH value
And change, show weaker EOF adjustability.And after poly-dopamine modification, the pipeline is in pH<When 5, EOF is in
Reversely, as pH >=5, EOF is constantly raised in forward direction, and EOF with the rise of pH value.Gather compared to unmodified
Ether ether ketone pipe and conventional vitreous silica capillary, all show stronger EOF adjustability.
In separation and detection that the device is used for various plants hormone, amino acid and aldehydes matter.As shown in figure 4,
Buffer solution is 5 mM ammonium acetate aqueous solutions（pH=7）, voltage is added under conditions of+15 kV, to realize in positive EOF bar
To the baseline separation of three Plant Hormones under part, managed compared to unmodified PEEK, separating effect lifting is obvious.Such as Fig. 5 institutes
Show, be 20 Mm ammonium acetate aqueous solutions in buffer solution（pH=3）, voltage is added under conditions of -15 kV, to realize in reverse electricity
To the baseline separation of three kinds of amino acid under the conditions of seepage flow.As shown in fig. 6 ,+15 kV voltage is being added using the pipeline, between pair
In the separation of two kinds of aldehydes matters of benzenediol and metanitrophenol, discovery is being added without acetonitrile（ACN）Under conditions of, resorcinol
Due to having certain hydrophobic interaction with poly-dopamine layer, the phenomenon of hangover is generated, in the slow of 20 Mm ammonium acetates (pH=7)
The ACN of addition 5% in fliud flushing, conditions of streaking is obviously improved.Continue raising ACN addition, the separation of two kinds of materials can be reduced
Degree.Managed compared to unmodified empty PEEK, the PEEK pipes of poly-dopamine modification have the work that improves significantly to separating effect
With.
Embodiment 2
1）The mg of dopamine 20 is weighed, is dissolved in 10 mmol/L Tris-HCl buffer solutions, pH to 9 is adjusted, solution is shaken, extremely
Solution is in light brown.
2）With the mL/min of flow velocity 0.08 by step 1）The solution of gained passes through 50 clean cm（360 μm of external diameter, internal diameter
50 μm）Polyether-ether-ketone pipe, modifies 24 h at room temperature, and water washing, nitrogen blows away solvent, is placed in 60 DEG C of h of drying in oven 3, obtains
Obtain the polyether-ether-ketone capillary electric chromatographic column of poly-dopamine modification.
Claims (3)
1. a kind of polyether-ether-ketone capillary electric chromatographic column of poly-dopamine modification, it is characterised in that be prepared by the following method
Arrive：
1）1 ~ 2 mg/mL dopamine alkaline solution is prepared, the pH of the solution is 8.5 ~ 9, logical oxygen makes its pre-oxidation to brown；
2）By step 1）Dopamine alkaline solution flowing after the pre-oxidation of gained passes through clean polyether-ether-ketone using peristaltic pump
Capillary column, the flow velocity of peristaltic pump is 0.01-0.1 mL/h, and the time of poly-dopamine modification is 1 ~ 24 hour, is placed in 60 DEG C of bakings
3 h of drying, are modified polyether-ether-ketone inside pipe wall in case.
2. the polyether-ether-ketone capillary electric chromatographic column of poly-dopamine modification according to claim 1 is in Capillary Electrophoresis-matter
Compose the application in combination analysis.
3. application according to claim 2, it is characterised in that refer to the polyether-ether-ketone capillary electricity of described poly-dopamine modification
Application of the chromatographic column in the capillary electrophoresis-laser-induced fluorescence separation analysis of amino acid, phenols and plant hormone analyte.
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| CN201710438672.4A CN107308924A (en) | 2017-06-12 | 2017-06-12 | A kind of polyether-ether-ketone capillary electric chromatographic column of poly-dopamine modification and its application |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115181314A (en) * | 2022-07-26 | 2022-10-14 | 季华实验室 | Method for surface modification of polyether-ether-ketone based on backbone grafting method |
| CN115216049A (en) * | 2022-07-27 | 2022-10-21 | 季华实验室 | Method for surface modification of polyether-ether-ketone based on grafting to main chain method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101913278A (en) * | 2010-06-30 | 2010-12-15 | 中国科学技术大学 | Preparation method of polydopamine-g-polyethylene oxide capillary coating for separating protein by capillary electrophoresis |
| CN103191705A (en) * | 2013-04-15 | 2013-07-10 | 武汉大学 | Preparation method of open tubular capillary electrochromatographic column |
| CN104359996A (en) * | 2014-12-02 | 2015-02-18 | 武汉大学 | Modified carbon fiber-filled polyether-ether-ketone pipe solid phase microextraction column and preparation method thereof |
| CN105214340A (en) * | 2015-09-14 | 2016-01-06 | 武汉长林医药科技有限公司 | A kind of method of fixing covalent organic framework material and application thereof |
| CN106076296A (en) * | 2016-05-31 | 2016-11-09 | 江西中医药大学 | Based on nickel foam adsorbent that poly-dopamine is modified and its preparation method and application |
-
2017
- 2017-06-12 CN CN201710438672.4A patent/CN107308924A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101913278A (en) * | 2010-06-30 | 2010-12-15 | 中国科学技术大学 | Preparation method of polydopamine-g-polyethylene oxide capillary coating for separating protein by capillary electrophoresis |
| CN103191705A (en) * | 2013-04-15 | 2013-07-10 | 武汉大学 | Preparation method of open tubular capillary electrochromatographic column |
| CN104359996A (en) * | 2014-12-02 | 2015-02-18 | 武汉大学 | Modified carbon fiber-filled polyether-ether-ketone pipe solid phase microextraction column and preparation method thereof |
| CN105214340A (en) * | 2015-09-14 | 2016-01-06 | 武汉长林医药科技有限公司 | A kind of method of fixing covalent organic framework material and application thereof |
| CN106076296A (en) * | 2016-05-31 | 2016-11-09 | 江西中医药大学 | Based on nickel foam adsorbent that poly-dopamine is modified and its preparation method and application |
Non-Patent Citations (3)
| Title |
|---|
| XUE-BO YIN ET AL.: ""Polydopamine-based permanent coating capillary electrochromatography for auxin determination"", 《JOURNAL OF CHROMATOGRAPHY A》 * |
| YOSHIHIDE TANAKA ET AL.: ""Development of a capillary electrophoresis-mass spectrometry method using polymer capillaried for metabolomic analysis of yeast"", 《ELECTROPHORESIS》 * |
| YOSHIHIDE TANAKA ET AL.: ""Development of a capillary electrophoresismass spectrometry method using polymer capillaries for metabolomic analysis of yeast"", 《ELECTROPHORESIS》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115181314A (en) * | 2022-07-26 | 2022-10-14 | 季华实验室 | Method for surface modification of polyether-ether-ketone based on backbone grafting method |
| CN115181314B (en) * | 2022-07-26 | 2024-05-07 | 季华实验室 | Method for carrying out surface modification on polyether-ether-ketone based on main chain grafting method |
| CN115216049A (en) * | 2022-07-27 | 2022-10-21 | 季华实验室 | Method for surface modification of polyether-ether-ketone based on grafting to main chain method |
| CN115216049B (en) * | 2022-07-27 | 2024-05-07 | 季华实验室 | Method for carrying out surface modification on polyether-ether-ketone based on grafting to main chain method |
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Application publication date: 20171103 |