CN107296824A - Comospore trifoliate jewelvine ligroin extraction and its preparation and inflammatory applications - Google Patents
Comospore trifoliate jewelvine ligroin extraction and its preparation and inflammatory applications Download PDFInfo
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- CN107296824A CN107296824A CN201710529969.1A CN201710529969A CN107296824A CN 107296824 A CN107296824 A CN 107296824A CN 201710529969 A CN201710529969 A CN 201710529969A CN 107296824 A CN107296824 A CN 107296824A
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- trifoliate jewelvine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
There is antiinflammatory action the invention discloses comospore trifoliate jewelvine ligroin extraction, have good result particular for the related inflammation of type ii diabetes.Testing result shows that giving the Chemokines such as TNF α, MCP 1, IL 6, the IL1 β of experimental model mouse of the treatment of comospore trifoliate jewelvine ligroin extraction and inflammatory factor significantly reduces, and inflammation mediators COX 2 expression declines in pancreas.Experimental study shows that comospore trifoliate jewelvine ligroin extraction of the present invention can significantly reduce inflammatory mediator and inflammatory Cytokines Expression, can improve insulin sensitivity by anti-inflammatory, treat insuline resistance syndrome.Therefore, the present invention provides theoretical foundation for the strong medicine comospore trifoliate jewelvine of further investigation and exploitation, to provide a kind of new modern Chinese herbal medicine preparation that diabetes are treated by antiinflammatory action to be clinical.
Description
Technical field
The invention belongs to strong medicine comospore trifoliate jewelvine technical field, more particularly to a kind of comospore trifoliate jewelvine ligroin extraction and its system
Standby and inflammatory applications.
Background technology
Diabetes, angiocardiopathy and tumour are that human lives are endangered with maximum disease.Diabetes are considered as the whole world
The disease problem faced.The condition lived due to modern people and the very big change of environment so that people suffer from diabetes probability
It is in rising trend.The data of international diabetes association issue point out, the year two thousand forty the whole world suffer from the patient of diabetes about to 6.42 hundred million people.
Therefore, find few side effects, efficiency high treatment diabetes medicament it is extremely urgent.
The metabolic disturbance diseases being characterized with diabetes, are also widely regarded as a kind of chronic inflammation disease.Grind
Study carefully and be proved to be the body as caused by many reasons to carbohydrate, fat and a kind of disorderly metabolic syndrome of protein metabolism.Sugar
The lasting hyperglycaemia of urine patient can promote inflammation process, trigger infringement and the systemic inflammatory response of body internal organs.
Comospore trifoliate jewelvine (Derris Eriocarpa How) is pulse family trifoliate jewelvine platymiscium, itself also known as native radix glycyrrhizae, main distribution
In areas such as Guangdong, Guangxi.Climb up by holding on to shape shrub, wooden big liana.Stem brown color, there is most yellow warty thrusts, and section is light
Yellow, threadiness.Winglike compound leaf alternate, leaflet is to life.Spend small, pink, in axillary-racemes.Sprig is micro- soft by rust
Hair, the florescence is the 6-7 months, and fruiting period is September to January in next year.Comospore trifoliate jewelvine is commonly used for treatment cystitis, ephritis, urethritis, cough
Deng having effects that promoting urination and removing dampness, relieving cough and reducing sputum.
The content of the invention
Should the technical problem to be solved in the present invention is to provide a kind of comospore trifoliate jewelvine ligroin extraction and its preparation and anti-inflammatory
With providing a kind of anti-inflammatory Chinese traditional with good efficacy newly to be clinical.
In order to solve the above technical problems, the present invention uses following technical scheme：
Comospore trifoliate jewelvine ligroin extraction, the petroleum ether extract part from comospore trifoliate jewelvine ethanol extract.
Application of the comospore trifoliate jewelvine ligroin extraction in anti-inflammatory drug is prepared.
Anti-inflammatory drug is anti-diabetic anti-inflammatory drugs.
Diabetes are type ii diabetes.
Application of the comospore trifoliate jewelvine ligroin extraction in treatment insuline resistance syndrome medicine is prepared.
Refluxing extraction after the preparation method of comospore trifoliate jewelvine ligroin extraction soaks comospore trifoliate jewelvine rattan using ethanol, it is dense
Contracting, flings to and comospore trifoliate jewelvine ethanol extract is obtained after ethanol；Comospore trifoliate jewelvine ethanol extract uses petroleum ether extraction, after concentration
To comospore trifoliate jewelvine ligroin extraction.
The preparation method of comospore trifoliate jewelvine ligroin extraction, is carried out by following operation：The section of comospore trifoliate jewelvine rattan will be dried,
Coarse powder is ground into through medicinal herb grinder, sieve to obtain comospore trifoliate jewelvine medicinal powder；Comospore trifoliate jewelvine medicinal powder is weighed, is soaked with ethanol after medicinal powder,
Heating and refluxing extraction, merges ethanol extract, and most ethanol is waved in concentration；Comospore trifoliate jewelvine ethanol extract is suspended with water, stone is added
Oily ether extraction, combining extraction liquid, concentration finally gives comospore trifoliate jewelvine ligroin extraction medicinal extract.
Cut into slices as 0.5-1cm, sieve as 30 mesh sieves.
Ethanol is the ethanol of volumetric concentration 95%, immersion medicinal powder 24h.
Heating and refluxing extraction 2 times, each 1h；Petroleum ether extraction 3 times.
To make full use of the distinctive strong medicine comospore trifoliate jewelvine in Guangxi, Yunnan, inventor establishes a kind of comospore trifoliate jewelvine petroleum ether
The preparation method of extract (Petroleum Ether Extract of Derris eriocarpa How., PEEDEH).According to
This, inventor further studies, it was found that comospore trifoliate jewelvine ligroin extraction has antiinflammatory action, particular for type ii diabetes
Related inflammation have good result.Type ii diabetes animal model is set up by using high glucose and high fat feed and Streptozotocin
(KM mouse), using ELISA to mice serum Chemokines and inflammatory factor TNF-α (TNF), MCP-1
(cell chemotaxis albumen), IL-6 (interleukin-6), IL1- β (interleukin-11 β) detect its content, using Immunohistochemical Method to mouse
Pancreas inflammatory mediating factors COX-2 is detected.Testing result is shown, gives the experiment of comospore trifoliate jewelvine ligroin extraction treatment
The Chemokines such as TNF-α, MCP-1, IL-6, IL1- β of model mice and inflammatory factor are significantly reduced, inflammation in pancreas
Mediating factors COX-2 expression declines.Experimental study shows that comospore trifoliate jewelvine ligroin extraction of the present invention can significantly reduce inflammation
Mediated factor and inflammatory Cytokines Expression, can improve insulin sensitivity by anti-inflammatory, treat insuline resistance syndrome.Cause
This, the present invention is that further investigation and the strong medicine comospore trifoliate jewelvine of exploitation provide theoretical foundation, to provide a kind of new lead to be clinical
Cross antiinflammatory action and treat the modern Chinese herbal medicine preparation of diabetes.
Brief description of the drawings
Fig. 1 is the inverted fluorescence microscope figure (400 ×) of each group mice pancreatic tissue COX-2 protein expressions.
Embodiment
First, the preparation of comospore trifoliate jewelvine ligroin extraction
Comospore trifoliate jewelvine rattan will be dried and be cut into 0.5-1cm sections, coarse powder is ground into through medicinal herb grinder, 30 mesh sieves are crossed and obtain hair
Fruit trifoliate jewelvine medicinal powder；Comospore trifoliate jewelvine medicinal powder 1kg is weighed, is soaked with the ethanol of volumetric concentration 95% after medicinal powder 24h, heating and refluxing extraction
2 times, each 1h merges ethanol extract, and concentration waves most ethanol, obtains comospore trifoliate jewelvine ethanol extract (92.5g)；By comospore
Trifoliate jewelvine ethanol extract is suspended with suitable quantity of water, adds 500mL petroleum ether extractions, is extracted 3 times, combining extraction liquid, concentration, final
To bottle-green comospore trifoliate jewelvine ligroin extraction medicinal extract 20g.
2nd, the foundation of experimental diabetes model mice
60 KM mouse of SPF grades of experimental animals, raise in experimental center Animal House, day alternates with night, mouse free water.Raise
Experiment office management and the correlation criterion of protection are observed in foster process.Experimental animal is purchased in Guangxi Medical University's zoopery
Center.Experiment KM mouse used are the maximum mouse of domestic usage amount, are commonly used for the research in terms of pharmacology, toxicology
And the production and calibrating of medicine, biological products.Test modeling and use high glucose and high fat forage feed and intraperitoneal injection chain urea assistant bacterium
The method of element sets up type ii diabetes model mice, possesses diabetic character.
Experiment packet is shown in Table 1.Experimental animal carries out gavage with 20mL/Kg volume.Normal group and model group are with equal
Volume gavage distilled water.21d is administered in animal, at the end of experiment, and predetermined processing mouse is handled by animal, collects mice plasma 4
DEG C, under the conditions of 12000rpm, centrifuge 10min.Mice pancreatic is taken, using brine, filter paper is blotted, and the two is together put
Enter -80 DEG C of refrigerators stand-by.
The experiment packet of table 1
Note：Positive drug is melbine, and dosage is 320mg/Kg
3rd, the detection of experimental animal Serum Indexes
Experimental procedure
Experimental animal serum is taken, is tested in strict accordance with the step in Testing index ELISA kit.Draw experiment examination
Agent box standard curve, sample-adding, incubation, washing, enzyme-added, incubation, washing, display, termination.Finally by calibration curve formula,
Calculate the mice serum index concentration of different groups.
Statistical procedures
Using the softwares of SPSS 21.0 carry out mathematical statistics, the comparison ANOVA one-way analysis of variances of multigroup, two-by-two
Compare use compare between LSD methods, group, organize in compare and counted with the t methods of inspection, experimental result is represented with x ± s, and P ＜ 0.05 recognize
To be statistically significant.
Experimental result
As shown in Table 2, comospore trifoliate jewelvine petroleum ether, which extracts medicinal extract, can significantly reduce the inflammation of (P ＜ 0.05) diabetic mice
Disease index TNF-α, MCP-1, IL-6, IL1- β, so as to reach the associated conditions for intervening diabetic mice inflammation.
The experimental animal Serum Indexes testing result of table 2
Compared with STZ group, * p ＜ 0.05
4th, animal pancreatic inflammation index COX-2 detection
Experimental procedure
Paraffin section 55 DEG C of bakings in baking box are taken, are made paraffin melting.In dimethylbenzene staining jar dewax 3 times, after with ladder
Spend in the alcohol for 10% and elute.Antigen retrieval, is closed using the corresponding serum of kit to slide, adds albumen correspondence
Primary antibody and secondary antibody carry out, secondary antibody closing, primary antibody be incubated overnight, washed using phosphate buffered saline solution, add coloring agent, dye
Color, is redyed, dehydration, reuses cover glass mounting.Observed using inverted fluorescence microscope under × 400 microscope multiple
Slide, determines the expression quantity of COX-2 in pancreatic tissue.
Experimental result
As shown in figure 1, the dyeing of model group Mice Islet Cells is obvious, the dyeing of positive drug and different pharmaceutical group islet cells
It has been mitigated that, dose-dependent relationship is presented in comospore trifoliate jewelvine oil ethereal extract low dosage, middle dosage, high dose, i.e., with medicine
The increase of thing medicinal extract concentration, the diabetic mice Intra-islet Apoptosis factor is reduced.Pancreatic secretion pancreatic juice, in sugar, protein
And played an important role in fat metabolism.Therefore, medicine can by reducing the expression of islet cells inflammatory factor COX-2 albumen, from
And improve diabetic disorders.
Claims (10)
1. comospore trifoliate jewelvine ligroin extraction, it is characterised in that the petroleum ether extract part from comospore trifoliate jewelvine ethanol extract.
2. application of the comospore trifoliate jewelvine ligroin extraction in anti-inflammatory drug is prepared described in claim 1.
3. the application according to its claim 2, it is characterised in that：The anti-inflammatory drug is anti-diabetic anti-inflammatory drugs.
4. the application according to its claim 3, it is characterised in that：The diabetes are type ii diabetes.
5. the answering in treatment insuline resistance syndrome medicine is prepared of comospore trifoliate jewelvine ligroin extraction described in claim 1
With.
6. the preparation method of comospore trifoliate jewelvine ligroin extraction described in claim 1, it is characterised in that：Comospore trifoliate jewelvine rattan is adopted
Refluxing extraction after being soaked with ethanol, concentration, flings to and comospore trifoliate jewelvine ethanol extract is obtained after ethanol；Comospore trifoliate jewelvine ethanol extract
Using petroleum ether extraction, comospore trifoliate jewelvine ligroin extraction is obtained after concentration.
7. the preparation method of comospore trifoliate jewelvine ligroin extraction according to claim 6, it is characterised in that by following operation
Carry out：The section of comospore trifoliate jewelvine rattan will be dried, coarse powder is ground into through medicinal herb grinder, sieve to obtain comospore trifoliate jewelvine medicinal powder；Weigh hair
Fruit trifoliate jewelvine medicinal powder, is soaked after medicinal powder, heating and refluxing extraction with ethanol, merges ethanol extract, and most ethanol is waved in concentration；By comospore
Trifoliate jewelvine ethanol extract is suspended with water, adds petroleum ether extraction, and combining extraction liquid, concentration finally gives comospore trifoliate jewelvine petroleum ether
Extract medicinal extract.
8. the preparation method of comospore trifoliate jewelvine ligroin extraction according to claim 7, it is characterised in that：The section is
0.5-1cm, sieves as 30 mesh sieves.
9. the preparation method of comospore trifoliate jewelvine ligroin extraction according to claim 7, it is characterised in that：The ethanol is
The ethanol of volumetric concentration 95%, immersion medicinal powder 24h.
10. the preparation method of comospore trifoliate jewelvine ligroin extraction according to claim 7, it is characterised in that：The heating
Refluxing extraction 2 times, each 1h；The petroleum ether extraction 3 times.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1753681A (en) * | 2003-02-26 | 2006-03-29 | 尤尼根制药公司 | Formulation for use in the prevention and treatment of carbohydrate induced diseases and conditions |
| WO2006111858A2 (en) * | 2005-01-14 | 2006-10-26 | Glaxosmithkline Istrazivacki Centar Zagreb D.O.O. | Bis-(coumarin) compounds with anti-inflammatory activity |
| CN104945408A (en) * | 2015-04-30 | 2015-09-30 | 广西民族大学 | Coumarin compounds, and preparation method and application thereof |
| CN104997768A (en) * | 2015-07-23 | 2015-10-28 | 上海市第六人民医院 | Application of rotenone in preparing blood glucose reduction medicine |
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2017
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1753681A (en) * | 2003-02-26 | 2006-03-29 | 尤尼根制药公司 | Formulation for use in the prevention and treatment of carbohydrate induced diseases and conditions |
| WO2006111858A2 (en) * | 2005-01-14 | 2006-10-26 | Glaxosmithkline Istrazivacki Centar Zagreb D.O.O. | Bis-(coumarin) compounds with anti-inflammatory activity |
| CN104945408A (en) * | 2015-04-30 | 2015-09-30 | 广西民族大学 | Coumarin compounds, and preparation method and application thereof |
| CN104997768A (en) * | 2015-07-23 | 2015-10-28 | 上海市第六人民医院 | Application of rotenone in preparing blood glucose reduction medicine |
| CN105362263A (en) * | 2015-12-05 | 2016-03-02 | 江苏省中国科学院植物研究所 | Application of compound Isomeranzin to treatment of inflammatory diseases |
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Application publication date: 20171027 |