CN107296059A

CN107296059A - A kind of utilization nitric oxide or nitric oxide donors improve method of the paddy rice to strip virus resistance

Info

Publication number: CN107296059A
Application number: CN201710445238.9A
Authority: CN
Inventors: 周彤; 周益军; 卢荣飞; 杜琳琳; 兰莹; 孙枫
Original assignee: Jiangsu Academy of Agricultural Sciences
Current assignee: Jiangsu Academy of Agricultural Sciences
Priority date: 2017-06-09
Filing date: 2017-06-09
Publication date: 2017-10-27

Abstract

One kind improves its method to rice stripe virus (Rice stripe virus, RSV) resistance using nitric oxide (Nitric oxide, NO) or NO donors pretreatment paddy rice, it is characterised in that：NO donor sodium nitroprussides (Sodium nitroprusside, SNP) concentration is 10 μM of 1mM, and the pretreatment paddy rice time is first 12 hours of inoculation.Resistance of the paddy rice to strip virus (Rice strip virus, RSV) can be heightened using this method, more economically the grain loss that the disease is caused can be reduced environmentally friendlyly using various fields such as agricultural production, scientific research and environmental protection.

Description

One kind improves paddy rice to strip virus resistance using nitric oxide or nitric oxide donors Method

Technical field：

The present invention uses nitric oxide (Nitric oxide, NO) or NO donors pretreatment paddy rice (Oryza sativa L.cv. resistance of the paddy rice to rice stripe virus (Rice stripe virus, RSV)) can be improved, agriculture life is can be applied to In production, belong to technical field of agriculture science.

Background technology：

RSV belongs to very thin Tobamovirus (Tenuivirus), and its vector is mainly small brown rice planthopper (Laodelphaxstriat Ellus) (SBPH), small brown rice planthopper once obtains malicious band poison throughout one's life, and can pass poison through ovum.Viral particle morphology is that branch is thread Body, genome is made up of four RNA, is respectively designated as RNA1, RNA2, RNA3 and RNA4, can encode virus response synthesis Or the protein of transfer correlation function.Wherein, the nucleocapsid protein (Nucleoprotein protein, NCP) of RNA3 codings The virus specified proteins (Specificity protein, SP) encoded with RNA4 play key in the pathogenic course of virus Effect.Yellow-white billet line occurs in early stage lobus cardiacus base portion, then slowly merges, and further becomes parallel with vein Normal green is still kept between yellow green striped, striped, overall is in yellowish green alternate striated.Under being crimped morbidity anaphase blade more Hang down, lobus cardiacus is withered, and symptom is small compared with light normal withered booting or fringe, solid seldom, serious symptom person death (Fig. 1).China's conduct The main producing region of world Southeast Asia paddy rice grain, the harm caused by the virosis is difficult to ignore, especially 1960s with Come, grain-production of the stripe virus disease on the south China especially band of Jiangsu and Zhejiang Provinces one causes very big influence.Therefore, rice stripe leaf The preventing and treating of rot is task that is one long-term and can not be ignored.

So far, stripe virus disease common preventive measures are mainly seed selection new disease-resistant varieties, or use chemical drugs Amboceptor plant hopper is killed in agent.Wherein, the seed selection workload of resistant variety is big, and time-consuming longer, the program is difficult to obtain in a short time Effect；And although chemical agent can be propagated with blocking virus, its is inefficient and is likely to result in environmental pollution.So, open The efficiently feasible and environmentally friendly control prece of hair is still our focus.

Nitric oxide (Nitric oxide, NO), is a kind of important gas molecules material in organism, is initially sent out Now in animal body, participate in many important physiological responses, including grow, be immunized, nerve conduction and cardiovascular response Deng.And in recent years, physiological roles of the NO in plant obtains the extensive concern of scientists, such as seed is sprouted, taken root, gas Hole switch, yield positive results and resistance response etc..Wherein, NO take part in many plant disease-resistant processes, for example antibacterium, fungi, Parasite and virus etc..Wherein, people focus mostly in dicotyledon, such as arabidopsis, tobacco for the research of plant disease-resistant With tomato etc., and research is limited to bacterium, fungi and parasite mostly, and the research of anti-virus aspect is relatively fewer.This just makes Although the research into theoretical side is very abundant, NO is but still not for the industrial crops viral diseases mechanism such as rice and wheat Foot.Studies have found that NO seems to resist pathogen with the common induction plant of the hormone of some in plant interaction Property, such as epiphysin (MT), salicylic acid (SA), jasmonic (JA), ethene (ET) and abscisic acid (ABA) hormonal readiness, induction are planted The up-regulation of disease-resistant original related gene in object.This explanation NO can promote the resistance that plant is infected to pathogen, and should During there may be different kinds of molecules mechanism, it is necessary to which our further researchs have shown that.

The content of the invention：

The present invention is directed to the studies above background, using paddy rice as research object, after the NO donors processing of certain gradient concentration Incidence is counted, control group water process, it is found that the incidence of disease of Nipponbare after NO processing is significantly lower than control group, wherein Effect reaches best at 100 μM.The invention provides the method for the stripe disease incidence of disease is reduced using NO, the party is utilized Method can heighten resistance of the paddy rice to RSV, can be using various fields such as agricultural production, scientific research and environmental protection, more economical environmental protection ground The grain loss that reduction disease is caused.

Brief description of the drawings：

Fig. 1 health rice plants and stripe disease plant

The malicious rate result of Fig. 2 Dot-ELISA detection small brown rice planthopper bands

(ND (No detected), which refers to, to be not detected at control group incidence of disease difference after Fig. 3 NO processing；Con refers to control； Different letters represent there is group difference in the levels of P ＜ 0.05).

Embodiment：

Method therefor is conventional method unless otherwise instructed in example below.

Embodiment 1, the method that paddy rice investigates its state of an illness is pre-processed using NO

1st, vegetable material, virus analysis and method is infected

Nipponbare (Oryza sativaL.cv.Nipponbare) seed uses 0.1%HgCl first₂Sterilize 1h；Then use Deionized water rinsing is clean, 25 DEG C of seed soaking 2d, vernalization 1d；Seed point after vernalization is sowed in 1L beakers and cultivated, 40 every glass, often Appropriate Hoagland nutrient solutions, illumination 14h, dark 10h, 28 ± 1 DEG C of temperature were poured every two days.

Carry RSV and nontoxic small brown rice planthopper is raised in Jiangsu Province Agriculture Science Institute Institute of Plant Protection insectary.After first-instar young hatching Divide and support on Wu-Yu-Geng 3 (Oryza sativaL.cv.Wuyujing NO.3) rice seedlings of health, be placed in 25 DEG C of insectaries In, three ages to four ages big small brown rice planthopper can be adsorbed for infection rice seedling using preceding malicious rate of its band with dot enzyme-linked immuno Test (Dot-ELISA) and determine (Fig. 2).

3rd, the incidence under different disposal

14d or so rice seedling, in the case where its soil is relatively dried, applies dH respectively₂O, various concentrations SNP solution (10,50,100,500,1000 μM) each 30mL (SNP is a kind of NO specific donors, is purchased from Sigma-Aldrich).Under dark 12h is handled overnight, poison is connect by effective inoculation worm amount/seedling of 4, is inoculated with 3d.Then worm is carefully scanned out, delayed in rice seedling room 1d is supported, then is transplanted to crop field, normal management.Incidence is checked within 1 month or so, the incidence of disease is counted.In triplicate.

As shown in Table, it has been found that compared to control group, NO processing can effectively alleviate the stripe disease state of an illness, The incidence of disease is reduced with concentrationdependent manner within the specific limits, wherein effect preferably, reduces about 23.30% at 100 μM The incidence of disease (P ＜ 0.05).Can with it is concluded that, external source addition NO can largely reduce the hair of stripe disease Sick rate.Further data analysis is as shown in Figure 3.

Above-mentioned implementation does not limit the present invention in any form.

Claims

1. one kind improves it to rice stripe disease using nitric oxide (Nitric oxide, NO) or NO donors pretreatment paddy rice The method of malicious (Rice stripe virus, RSV) resistance, it is characterised in that：NO donor sodium nitroprussides (Sodium Nitroprusside, SNP) concentration is 10 μM of -1mM, the pretreatment paddy rice time is first 12 hours of inoculation.