CN107290509A - A kind of method traced to the source based on stable isotope technology the wool fiber place of production - Google Patents
A kind of method traced to the source based on stable isotope technology the wool fiber place of production Download PDFInfo
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- CN107290509A CN107290509A CN201710660312.9A CN201710660312A CN107290509A CN 107290509 A CN107290509 A CN 107290509A CN 201710660312 A CN201710660312 A CN 201710660312A CN 107290509 A CN107290509 A CN 107290509A
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- 210000002268 wool Anatomy 0.000 title claims abstract description 73
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 34
- 238000005516 engineering process Methods 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 25
- 239000000835 fiber Substances 0.000 title claims abstract description 17
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 26
- 241001494479 Pecora Species 0.000 claims abstract description 22
- 239000001301 oxygen Substances 0.000 claims abstract description 16
- 239000003651 drinking water Substances 0.000 claims abstract description 13
- 235000020188 drinking water Nutrition 0.000 claims abstract description 13
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000007689 inspection Methods 0.000 claims abstract description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 42
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 238000001514 detection method Methods 0.000 claims description 15
- 229910052739 hydrogen Inorganic materials 0.000 claims description 15
- 239000011259 mixed solution Substances 0.000 claims description 11
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 10
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims description 10
- 229910052799 carbon Inorganic materials 0.000 claims description 10
- 239000008367 deionised water Substances 0.000 claims description 10
- 229910021641 deionized water Inorganic materials 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 10
- 238000000227 grinding Methods 0.000 claims description 10
- 229910052757 nitrogen Inorganic materials 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 10
- 239000007787 solid Substances 0.000 claims description 10
- 238000007873 sieving Methods 0.000 claims description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 5
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 5
- 241001236644 Lavinia Species 0.000 claims description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 5
- 238000007405 data analysis Methods 0.000 claims description 5
- 238000005238 degreasing Methods 0.000 claims description 5
- 239000001257 hydrogen Substances 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 5
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims description 5
- 238000004321 preservation Methods 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims 1
- 238000002347 injection Methods 0.000 claims 1
- 239000007924 injection Substances 0.000 claims 1
- 230000000155 isotopic effect Effects 0.000 abstract description 6
- 239000004753 textile Substances 0.000 abstract description 4
- 238000004458 analytical method Methods 0.000 abstract description 3
- 239000004744 fabric Substances 0.000 abstract description 3
- 239000002207 metabolite Substances 0.000 abstract description 3
- 238000004445 quantitative analysis Methods 0.000 abstract description 3
- 230000009466 transformation Effects 0.000 abstract description 2
- 102000011782 Keratins Human genes 0.000 description 7
- 108010076876 Keratins Proteins 0.000 description 7
- 230000004060 metabolic process Effects 0.000 description 4
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 239000000460 chlorine Substances 0.000 description 3
- 229910052801 chlorine Inorganic materials 0.000 description 3
- 238000007654 immersion Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000000474 nursing effect Effects 0.000 description 3
- 241000209149 Zea Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/36—Textiles
- G01N33/362—Material before processing, e.g. bulk cotton or wool
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0098—Plants or trees
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Textile Engineering (AREA)
- Botany (AREA)
- Wood Science & Technology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention relates to textile inspection field, a kind of method traced to the source based on stable isotope technology the wool fiber place of production is disclosed.The present invention is traced to the source the woolen place of production using stable isotope technology, by being measured to the Hydrogen-oxygen Isotope value in the hydrogen-oxygen carbon nitrogen isotope value in wool, feed and drinking-water, result finally is carried out into clustering, the woolen place of production is traced to the source.The present invention can determine the transfer and transformation of metabolite accurate quantitative analysis using Stable Isotopic Analysis technology, so as to carry out the stable source tracing method of a science to sheep and the wool fabric place of production.
Description
Technical field
The present invention relates to textile inspection field, more particularly to one kind based on stable isotope technology to wool fiber
The method that the place of production is traced to the source.
Background technology
In recent years, rapid growth trend is presented in China's production of wool.With the improvement of living standards, people increasingly pay attention to
The quality of textile, but gradually woolen market occur in that adulterate, practise fraud, a series of quality such as uneven do not conform to
The problems such as lattice.The quality of the quality of wool fibre is directly connected to the quality and yield of downstream textile, traditional wool fibre quality
Detection method is artificial experience diagnostic method, is influenceed larger by people's subjective factor, is badly in need of setting up effective, perfect sheep and wool traces back
Source system, meets the demand for carrying out Fast Classification in market to wool, divide wool original producton location, differentiate wool quality it is good and bad,
Played a significant role in terms of the order of specification wool trade market.
All contain keratin in animal hair, and keratin once grows up to, and its structure and composition change all without with the time
Become, can effectively record ingest information of the animal within the corresponding time.This is that the animal history of life and the place of production are traced to the source there is provided possibility, by
Live body sampling can be achieved in each tissue rich in keratin, convenient, fast and information is accurate, in recent years, gradually by the place of production
Trace to the source the favor of researcher.Isotopic traceability technology is mainly set up with the place of production and contacted, and can distinguish variety classes, separate sources
Biological product, be to judge a kind of relatively directly more effective method in region source again.From, physicist Thomson in 1912
It was found that since stable isotope, the technology is widely used to medical science, biology, Food Science, environmental science, hydrogeology
Etc. traditional field, stable isotope technology had also developed into tracing to the source for special dimension in recent years, and the author utilizes stable isotope skill
Art is traced to the source the woolen place of production.
The content of the invention
In order to solve the above-mentioned technical problem, the invention provides one kind based on stable isotope technology to wool fiber
The method that the place of production is traced to the source.The present invention is traced to the source the woolen place of production using stable isotope technology, by wool, feed
Hydrogen-oxygen carbon nitrogen isotope value and drinking-water in Hydrogen-oxygen Isotope value be measured, result is finally subjected to clustering, it is right
Traced to the source in the woolen place of production.
Due in nature organism and external environment constantly carry out in mass exchange, organism isotopics can because
Nature fractionation effect occurs for geographical position, weather, environment, the influence of biological metabolism type, causes the organism of separate sources same
Position element composition has differences, and the isotope " fingerprint " of organism can carry the organism local environment factor and organism metabolism class
Type, wool is mainly made up of keratin, and keratin is more stable, can be effectively anti-once long achievement is no longer exchanged with other tissues
The feeding information of sheep is reflected, therefore can determine the transfer of metabolite using Stable Isotopic Analysis technology accurate quantitative analysis and turn
Become, so as to carry out the stable source tracing method of a science to sheep and the wool fabric place of production.
The present invention concrete technical scheme be：It is a kind of based on stable isotope technology the wool fiber place of production to be traced to the source
Method, comprises the following steps：
1）The sheep in some representational sheep producing regions is selected, 1 ~ 2cm of the clip at cortex wool hitches root,
And mark respectively and be fitted into preservation in valve bag.
The present invention chooses the wool at the cortex, it is advantageous that according to sheep in local nursing time, and wool
The minimum speed of growth（1.5cm/ the moon）, choose section that is close basic and can effectively reflecting nursing information, effectively correspondence nursing
Feed information, improve the accuracy traced to the source
2）The feed and drinking water in nearest one month of above-mentioned regional sheep are collected respectively, and are marked and be fitted into valve bag respectively
Preserve.
3）The wool of collection is soaked with deionized water respectively by area, cleaned, the freeze-day with constant temperature 10 in 60 ~ 80 DEG C of baking ovens
~ 12h, 2 ~ 4h degreasings are soaked then at the mixed solution of methanol and chloroform, and 30 ~ 60min is soaked in cleaning, again with methanol and chloroform
Mixed solution soaks, and finally, is cleaned, is dried at 60 ~ 80 DEG C with deionized water, standby.
The present invention uses methanol:The mixed solution of chloroform is handled, it is advantageous that in the presence of certain moisture, polarity
Methanol mixes liquid energy with nonpolar chloroform and efficiently extracts whole lipids, including free fat and combines fat.
4）By step 2）In feed respectively by area be placed in baking oven dry.
5）From step 3）In in dried wool a random crawl part ground with ball mill, the wool powder after grinding
Sieving, is then transferred in valve bag, is put after mark in people's drier and preserve to be measured.
6）From step 4）A random crawl part, is fully ground, the powder after grinding with ball mill in dried feed
Sieving, is then transferred in valve bag, is put after mark in people's drier and preserve to be measured.
7）Respectively by step 5）With step 6）In wool, feed be put on balancing stand, balance 90- under room temperature environment
After 100h, separately sampled product 0.8-1.2mg is respectively fed to elemental analyser after being wrapped with tinfoil paper cup by automatic sampler
Carbon and nitrogen in Flash HT 2000, this sample are converted into pure CO and N2.Solid C, N are carried out into system
Isotope detection.
8）Step 4 is taken respectively）With step 5）In wool, feed 0.8-1.2mg, with tinfoil paper cup wrap after by adopting automatically
Sample device is respectively fed to hydrogen and oxygen in elemental analyser Flash HT 2000, this sample by the vitrifying carbon granules in reacting furnace also
Originally it was H2 and CO；Carry out solid H, O isotope detection.
9）With standard syringe aspiration step 2）The drinking water of middle collection, crosses 0.2 μm of water system filter membrane, fills 2mL sample introduction
Bottle.Through H, O in automatic sample handling system detection water.
10）Data analysis：Clustering is carried out to four kinds of isotope values of hydrogen-oxygen carbon nitrogen, the wool of different regions is subjected to area
Point, set up database.
11）Measuring samples are gathered, are detected by preceding method, comparison database, the wool place of production is carried out and traces to the source.
Preferably, step 2）In, the feed is herbage, corn.
Preferably, step 3）In, the volume ratio of the methanol and chloroform is 2:1.
Preferably, step 4）In, drying temperature is 70 DEG C -100 DEG C, and drying time is 2-4h.
Preferably, step 5）With step 6）In, it is 80-120 mesh to cross grit number.
Preferably, step 7）, step 8）, step 9）In, each sample is repeated to survey three times and averaged.
It is compared with the prior art, the beneficial effects of the invention are as follows：
The present invention is traced to the source the woolen place of production using stable isotope technology, by the hydrogen-oxygen carbon nitrogen in wool, feed
Hydrogen-oxygen Isotope value in isotope value and drinking-water is measured, and result finally is carried out into clustering, to the woolen place of production
Traced to the source.
Due in nature organism and external environment constantly carry out in mass exchange, organism isotopics can because
Nature fractionation effect occurs for geographical position, weather, environment, the influence of biological metabolism type, causes the organism of separate sources same
Position element composition has differences, and the isotope " fingerprint " of organism can carry the organism local environment factor and organism metabolism class
Type, wool is mainly made up of keratin, and keratin is more stable, can be effectively anti-once long achievement is no longer exchanged with other tissues
The feeding information of sheep is reflected, therefore can determine the transfer of metabolite using Stable Isotopic Analysis technology accurate quantitative analysis and turn
Become, so as to carry out the stable source tracing method of a science to sheep and the wool fabric place of production.
Embodiment
With reference to embodiment, the invention will be further described.
Embodiment 1
A kind of method traced to the source based on stable isotope technology the wool fiber place of production, comprises the following steps：
1）The sheep in some representational sheep producing regions is selected, 1 ~ 2cm of the clip at cortex wool hitches root,
And mark respectively and be fitted into preservation in valve bag.
2）The feed in nearest one month of above-mentioned regional sheep is collected respectively（Herbage）And drinking water, and mark and fill respectively
Enter in valve bag and preserve.
3）The wool of collection is soaked with deionized water respectively by area, cleaned, the freeze-day with constant temperature 11h in 70 DEG C of baking ovens,
Then at methanol and the mixed solution of chloroform（Volume ratio is 2:1）3h degreasings are soaked, 45min, again with methanol and chlorine are soaked in cleaning
Imitative mixed solution immersion, finally, is cleaned with deionized water, is dried at 70 DEG C, standby.
4）By step 2）In feed respectively by area be placed in baking oven dry.Drying temperature is 85 DEG C, and drying time is
3h。
5）From step 3）In in dried wool a random crawl part ground with ball mill, the wool powder after grinding
Sieving（100 mesh）, it is then transferred in valve bag, is put after mark in people's drier and preserve to be measured.
6）From step 4）A random crawl part, is fully ground, the powder after grinding with ball mill in dried feed
Sieving（100 mesh）, it is then transferred in valve bag, is put after mark in people's drier and preserve to be measured.
7）Respectively by step 5）With step 6）In wool, feed be put on balancing stand, balance 95h under room temperature environment
Afterwards, separately sampled product 1mg, elemental analyser Flash HT 2000 are respectively fed to after being wrapped with tinfoil paper cup by automatic sampler,
Carbon and nitrogen in this sample are converted into pure CO and N2.Solid C, N isotope detection is carried out into system.Each
Sample is repeated to survey three times and averaged.
8）Step 4 is taken respectively）With step 5）In wool, feed 1mg, pass through automatic sampler point after being wrapped with tinfoil paper cup
Not Song Ru elemental analyser Flash HT 2000, hydrogen and oxygen in this sample are reduced to H by the vitrifying carbon granules in reacting furnace2
And CO；Carry out solid H, O isotope detection.Each sample is repeated to survey three times and averaged.
9）With standard syringe aspiration step 2）The drinking water of middle collection, crosses 0.2 μm of water system filter membrane, fills 2mL sample introduction
Bottle.Through H, O in automatic sample handling system detection water.Each sample is repeated to survey three times and averaged.
10）Data analysis：Clustering is carried out to four kinds of isotope values of hydrogen-oxygen carbon nitrogen, the wool of different regions is subjected to area
Point, set up database.
11）Measuring samples are gathered, are detected by preceding method, comparison database, the wool place of production is carried out and traces to the source.
Embodiment 2
A kind of method traced to the source based on stable isotope technology the wool fiber place of production, comprises the following steps：
1）The sheep in some representational sheep producing regions is selected, 1 ~ 2cm of the clip at cortex wool hitches root,
And mark respectively and be fitted into preservation in valve bag.
2）The feed in nearest one month of above-mentioned regional sheep is collected respectively（Corn）And drinking water, and mark and fill respectively
Enter in valve bag and preserve.
3）The wool of collection is soaked with deionized water respectively by area, cleaned, the freeze-day with constant temperature 12h in 60 DEG C of baking ovens,
Then at methanol and the mixed solution of chloroform（Volume ratio is 2:1）2h degreasings are soaked, 30min, again with methanol and chlorine are soaked in cleaning
Imitative mixed solution immersion, finally, is cleaned with deionized water, is dried at 60 ~ 80 DEG C, standby.
4）By step 2）In feed respectively by area be placed in baking oven dry.Drying temperature is 70 DEG C, and drying time is
4h。
5）From step 3）In in dried wool a random crawl part ground with ball mill, the wool powder after grinding
Sieving（80 mesh）, it is then transferred in valve bag, is put after mark in people's drier and preserve to be measured.
6）From step 4）A random crawl part, is fully ground, the powder after grinding with ball mill in dried feed
Sieving（80 mesh）, it is then transferred in valve bag, is put after mark in people's drier and preserve to be measured.
7）Respectively by step 5）With step 6）In wool, feed be put on balancing stand, balance 90h under room temperature environment
Afterwards, separately sampled product 1mg, elemental analyser Flash HT 2000 are respectively fed to after being wrapped with tinfoil paper cup by automatic sampler,
Carbon and nitrogen in this sample are converted into pure CO and N2.Solid C, N isotope detection is carried out into system.Each
Sample is repeated to survey three times and averaged.
8）Step 4 is taken respectively）With step 5）In wool, feed 1mg, pass through automatic sampler point after being wrapped with tinfoil paper cup
Not Song Ru elemental analyser Flash HT 2000, hydrogen and oxygen in this sample are reduced to H by the vitrifying carbon granules in reacting furnace2
And CO；Carry out solid H, O isotope detection.Each sample is repeated to survey three times and averaged.
9）With standard syringe aspiration step 2）The drinking water of middle collection, crosses 0.2 μm of water system filter membrane, fills 2mL sample introduction
Bottle.Through H, O in automatic sample handling system detection water.Each sample is repeated to survey three times and averaged.
10）Data analysis：Clustering is carried out to four kinds of isotope values of hydrogen-oxygen carbon nitrogen, the wool of different regions is subjected to area
Point, set up database.
11）Measuring samples are gathered, are detected by preceding method, comparison database, the wool place of production is carried out and traces to the source.
Embodiment 3
A kind of method traced to the source based on stable isotope technology the wool fiber place of production, comprises the following steps：
1）The sheep in some representational sheep producing regions is selected, 1 ~ 2cm of the clip at cortex wool hitches root,
And mark respectively and be fitted into preservation in valve bag.
2）The feed in nearest one month of above-mentioned regional sheep is collected respectively（Herbage）And drinking water, and mark and fill respectively
Enter in valve bag and preserve.
3）The wool of collection is soaked with deionized water respectively by area, cleaned, the freeze-day with constant temperature 10h in 80 DEG C of baking ovens,
Then at methanol and the mixed solution of chloroform（Volume ratio is 2:1）4h degreasings are soaked, 60min, again with methanol and chlorine are soaked in cleaning
Imitative mixed solution immersion, finally, is cleaned with deionized water, is dried at 80 DEG C, standby.
4）By step 2）In feed respectively by area be placed in baking oven dry.Drying temperature is 100 DEG C, and drying time is
2h。
5）From step 3）In in dried wool a random crawl part ground with ball mill, the wool powder after grinding
Sieving（120 mesh）, it is then transferred in valve bag, is put after mark in people's drier and preserve to be measured.
6）From step 4）A random crawl part, is fully ground, the powder after grinding with ball mill in dried feed
Sieving（120 mesh）, it is then transferred in valve bag, is put after mark in people's drier and preserve to be measured.
7）Respectively by step 5）With step 6）In wool, feed be put on balancing stand, balance 100h under room temperature environment
Afterwards, separately sampled product 1mg, elemental analyser Flash HT 2000 are respectively fed to after being wrapped with tinfoil paper cup by automatic sampler,
Carbon and nitrogen in this sample are converted into pure CO and N2.Solid C, N isotope detection is carried out into system.Each
Sample is repeated to survey three times and averaged.
8）Step 4 is taken respectively）With step 5）In wool, feed 1mg, pass through automatic sampler point after being wrapped with tinfoil paper cup
Not Song Ru elemental analyser Flash HT 2000, hydrogen and oxygen in this sample are reduced to H by the vitrifying carbon granules in reacting furnace2
And CO；Carry out solid H, O isotope detection.Each sample is repeated to survey three times and averaged.
9）With standard syringe aspiration step 2）The drinking water of middle collection, crosses 0.2 μm of water system filter membrane, fills 2mL sample introduction
Bottle.Through H, O in automatic sample handling system detection water.Each sample is repeated to survey three times and averaged.
10）Data analysis：Clustering is carried out to four kinds of isotope values of hydrogen-oxygen carbon nitrogen, the wool of different regions is subjected to area
Point, set up database.
11）Measuring samples are gathered, are detected by preceding method, comparison database, the wool place of production is carried out and traces to the source.
Raw materials used in the present invention, equipment, is the conventional raw material, equipment of this area unless otherwise noted；In the present invention
Method therefor, is the conventional method of this area unless otherwise noted.
It is described above, only it is presently preferred embodiments of the present invention, not the present invention is imposed any restrictions, it is every according to the present invention
Any simple modification, change and equivalent transformation that technical spirit is made to above example, still fall within the technology of the present invention side
The protection domain of case.
Claims (6)
1. a kind of method traced to the source based on stable isotope technology the wool fiber place of production, it is characterised in that including following step
Suddenly：
1）The sheep in some representational sheep producing regions is selected, 1 ~ 2cm of the clip at cortex wool hitches root,
And mark respectively and be fitted into preservation in valve bag；
2）The feed and drinking water in nearest one month of above-mentioned regional sheep are collected respectively, and are marked and be fitted into valve bag respectively
Preserve；
3）The wool of collection is soaked with deionized water, cleaned by area respectively, in 60 ~ 80 DEG C of baking ovens freeze-day with constant temperature 10 ~
12h, 2 ~ 4h degreasings are soaked then at the mixed solution of methanol and chloroform, and 30 ~ 60min is soaked in cleaning, again with methanol and chloroform
Mixed solution soaks, and finally, is cleaned, is dried at 60 ~ 80 DEG C with deionized water, standby；
4）By step 2）In feed respectively by area be placed in baking oven dry；
5）From step 3）In in dried wool a random crawl part ground with ball mill, the wool powder mistake after grinding
Sieve, is then transferred in valve bag, is put after mark in people's drier and preserve to be measured；
6）From step 4）A random crawl part, is fully ground with ball mill in dried feed, the powder sieving after grinding,
It is then transferred in valve bag, is put after mark in people's drier and preserve to be measured；
7）Respectively by step 5）With step 6）In wool, feed be put on balancing stand, balance 90-100h under room temperature environment
Afterwards, separately sampled product 0.8-1.2mg, elemental analyser Flash HT are respectively fed to after being wrapped with tinfoil paper cup by automatic sampler
2000, carbon and nitrogen in this sample are converted into pure CO and N2；The inspection of solid C, N isotope is carried out into system
Survey；
8）Step 4 is taken respectively）With step 5）In wool, feed 0.8-1.2mg, with tinfoil paper cup wrap after by automatic sampler
The hydrogen and oxygen being respectively fed in elemental analyser Flash HT 2000, this sample are reduced to by the vitrifying carbon granules in reacting furnace
H2And CO；Carry out solid H, O isotope detection；
9）With standard syringe aspiration step 2）The drinking water of middle collection, crosses 0.2 μm of water system filter membrane, fills 2mL sample injection bottle；Through
H, O in automatic sample handling system detection water；
10）Data analysis：Clustering is carried out to four kinds of isotope values of hydrogen-oxygen carbon nitrogen, the wool of different regions is made a distinction,
Set up database；
11）Measuring samples are gathered, are detected by preceding method, comparison database, the wool place of production is carried out and traces to the source.
2. a kind of method traced to the source based on stable isotope technology the wool fiber place of production as claimed in claim 1, its
It is characterised by, step 2）In, the feed is herbage, corn.
3. a kind of method traced to the source based on stable isotope technology the wool fiber place of production as claimed in claim 1, its
It is characterised by, step 3）In, the volume ratio of the methanol and chloroform is 2:1.
4. a kind of method traced to the source based on stable isotope technology the wool fiber place of production as claimed in claim 1, its
It is characterised by, step 4）In, drying temperature is 70 DEG C -100 DEG C, and drying time is 2-4h.
5. a kind of method traced to the source based on stable isotope technology the wool fiber place of production as claimed in claim 1, its
It is characterised by, step 5）With step 6）In, it is 80-120 mesh to cross grit number.
6. a kind of method traced to the source based on stable isotope technology the wool fiber place of production as claimed in claim 1, its
It is characterised by, step 7）, step 8）, step 9）In, each sample is repeated to survey three times and averaged.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710660312.9A CN107290509A (en) | 2017-08-04 | 2017-08-04 | A kind of method traced to the source based on stable isotope technology the wool fiber place of production |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710660312.9A CN107290509A (en) | 2017-08-04 | 2017-08-04 | A kind of method traced to the source based on stable isotope technology the wool fiber place of production |
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| Publication Number | Publication Date |
|---|---|
| CN107290509A true CN107290509A (en) | 2017-10-24 |
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| CN201710660312.9A Pending CN107290509A (en) | 2017-08-04 | 2017-08-04 | A kind of method traced to the source based on stable isotope technology the wool fiber place of production |
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