CN107272218A - High-speed structures photoimaging systems - Google Patents
High-speed structures photoimaging systems Download PDFInfo
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- CN107272218A CN107272218A CN201710386923.9A CN201710386923A CN107272218A CN 107272218 A CN107272218 A CN 107272218A CN 201710386923 A CN201710386923 A CN 201710386923A CN 107272218 A CN107272218 A CN 107272218A
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/58—Optics for apodization or superresolution; Optical synthetic aperture systems
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- G—PHYSICS
- G02—OPTICS
- G02F—OPTICAL DEVICES OR ARRANGEMENTS FOR THE CONTROL OF LIGHT BY MODIFICATION OF THE OPTICAL PROPERTIES OF THE MEDIA OF THE ELEMENTS INVOLVED THEREIN; NON-LINEAR OPTICS; FREQUENCY-CHANGING OF LIGHT; OPTICAL LOGIC ELEMENTS; OPTICAL ANALOGUE/DIGITAL CONVERTERS
- G02F1/00—Devices or arrangements for the control of the intensity, colour, phase, polarisation or direction of light arriving from an independent light source, e.g. switching, gating or modulating; Non-linear optics
- G02F1/01—Devices or arrangements for the control of the intensity, colour, phase, polarisation or direction of light arriving from an independent light source, e.g. switching, gating or modulating; Non-linear optics for the control of the intensity, phase, polarisation or colour
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- G—PHYSICS
- G02—OPTICS
- G02F—OPTICAL DEVICES OR ARRANGEMENTS FOR THE CONTROL OF LIGHT BY MODIFICATION OF THE OPTICAL PROPERTIES OF THE MEDIA OF THE ELEMENTS INVOLVED THEREIN; NON-LINEAR OPTICS; FREQUENCY-CHANGING OF LIGHT; OPTICAL LOGIC ELEMENTS; OPTICAL ANALOGUE/DIGITAL CONVERTERS
- G02F1/00—Devices or arrangements for the control of the intensity, colour, phase, polarisation or direction of light arriving from an independent light source, e.g. switching, gating or modulating; Non-linear optics
- G02F1/29—Devices or arrangements for the control of the intensity, colour, phase, polarisation or direction of light arriving from an independent light source, e.g. switching, gating or modulating; Non-linear optics for the control of the position or the direction of light beams, i.e. deflection
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- Physics & Mathematics (AREA)
- Nonlinear Science (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Optical Modulation, Optical Deflection, Nonlinear Optics, Optical Demodulation, Optical Logic Elements (AREA)
Abstract
A kind of high-speed structures photoimaging systems, including：Pulse optical signal generating device, optical circulator, spatial dispersion device, spatial light modulator and photoimaging equipment；Pulse optical signal generating device, for producing pulsed optical signals；Optical circulator, for adjusting light path, so as to enter the first light path from the incident pulsed optical signals of pulse optical signal generating device through optical circulator, then is returned, and incide photoimaging equipment from the first light path；Spatial dispersion device, in the first light path, for by from the frequency spectrum of the incident pulsed optical signals of optical circulator in space development；Spatial light modulator, in the first light path, for will be modulated in the optical signal of space development；Optical signal after photoimaging equipment, utilization space light modulator modulates is imaged to target object.The present invention improves the time needed for one vertical frame dimension resolution image of reconstruct, finally realizes the structure light super-resolution imaging system of a high speed.
Description
Technical field
The present embodiments relate to a kind of high-speed structures photoimaging systems.
Background technology
For many years, the microscopical resolution limitations of wide field/confocal fluorescent are in Abbe/Rayleigh limit of light, it is impossible to differentiate
Go out below 200nm structure, and super-resolution optical imaging technique has broken the limitation, is provided effectively for life science
Instrument.Current super-resolution imaging technology mainly includes the super-resolution micro imaging method based on single molecular imaging, and such as light swashs
Positioning microtechnic (photoactivated localization microscopy, PALM) living and random optical reconstruct are micro-
Technology (stochastic optical reconstruction microscopy, STORM), and by transforming the point of light source
Spread function is come the method that improves imaging resolution, including stimulated emission depletion microtechnic (stimulated emission
Deplet ion, STED) and saturated structures illumination microtechnic (saturated structure illumination
Microscopy, SSIM) compared with first three super-resolution imaging technology, although the two of SIM resolution ratio only conventional microscope
Times (100nm), but it possesses the advantage that the fast and required illumination light intensity of image taking speed is far smaller than other methods, therefore structure
Light technology provides possibility for active somatic cell imaging.But existing structure light imaging method image taking speed is slow, seriously governs
The imaging frame rate of structured-light system.
The content of the invention
The purpose of the embodiment of the present invention is to provide a kind of high-speed structures photoimaging systems, to solve above-mentioned technical problem.
According at least one embodiment of the present invention there is provided a kind of high-speed structures photoimaging systems, including：Pulsed light is believed
Number generating means, optical circulator, spatial dispersion device, spatial light modulator and photoimaging equipment；The pulsed optical signals hair
Generating apparatus, for producing pulsed optical signals；The optical circulator, for adjusting light path, so as to occur from the pulsed optical signals
The incident pulsed optical signals of device enter the first light path through the optical circulator, then are returned from first light path, are incorporated to
It is mapped to the photoimaging equipment；Spatial dispersion device, in first light path, for will be incident from the optical circulator
The frequency spectrum of pulsed optical signals is in space development；Spatial light modulator, it is described in space exhibition for inciting somebody to action in first light path
The optical signal opened is modulated；Photoimaging equipment, the optical signal after being modulated using the spatial light modulator is entered to target object
Row imaging.
For example, the pulse optical signal generating device is wide range light-pulse generator, short-pulse light source or DC light source.
For example, the spatial light modulator is modulated to the direction in space of the light.
For example, the spatial light modulator is modulated to the phase of the light.
For example, the spatial light modulator is modulated to the spatial frequency amplitude of light.
For example, also including wave filter, the wave filter is used for ripple of the light based on light for deploying the spatial dispersion device
Length is filtered, to be divided into different-waveband；The difference that the spatial light modulator is divided into based on wave band to the wave filter
The light of wave band is modulated.
For example, the spatial light modulator is by the light modulation of the different-waveband to three different direction in spaces.
For example, the spatial light modulator by the spatial frequency modulation of the light of the different-waveband into three different phases
Position.
For example, the spatial light modulator be digital micromirror array, by modulate the digital micromirror array direction and
Angle, come the direction of the light of modulating the injection and phase.
For example, the spatial light modulator is fixed pattern mask plate, based on the pattern of the fixed pattern mask plate, come
Modulate direction and the phase of the light of the injection.
11st, the photoimaging systems according to claim 9 or 10, wherein, the spatial light modulator modulates difference
Pattern, so that the light for inciding the spatial light modulator has different direction in space and phase.
For example, the pattern is striped.
For example, the striped includes at least two.
For example, at least two striped includes three kinds of stripeds or more than three kinds stripeds.
For example, at least two striped includes at least two in horizontal stripe, nicking and inclined stripe.
For example, the spatial light modulator is based on different luminous intensities, it is different pattern by the light modulation of the different-waveband.
For example, the spatial light modulator is based on the color do not shared the same light, it is different figures by the light modulation of the different-waveband
Case.
For example, also including lens, the lens are located between the wave filter and the spatial light modulator, described
Mirror is converged the light of the filters filter, and the spatial light modulator is carried out to the light of the different-waveband after the convergence
Modulation.
For example, the photoimaging equipment includes object lens, the object lens are utilized after the modulation returned from first light path
Light is imaged to the target object.
For example, the object lens include the first object lens and the second object lens, respectively positioned at the both sides of the target object；It is described
First object lens receive the light after the modulation returned from first light path and incide the target object, second object lens pair
The reflected light and/or scattered light of the target object are acquired.
For example, the photoimaging equipment also includes the second Dispersive Devices, the light for being gathered to second object lens is carried out
Dispersion.
For example, the photoimaging equipment also includes the second lens, second lens are gathered to second Dispersive Devices
Light converged.
For example, the photoimaging equipment also includes photo-sensitive cell, for being carried out into the light gathered from the target object
Picture.
For example, the photo-sensitive cell includes fundamental component and positive negative frequency shift point from the optical signal that the target object is gathered
The linear combination of amount.
For example, the linear coefficient of the frequency-shifted components is related to the phase for the pattern that the spatial light adjuster is modulated.
For example, the spatial light modulator by the spatial frequency modulation of the light of the different-waveband into three different phases
Position, the photo-sensitive cell is acquired based on the light that three different phases reflect the target object and/or scattered,
Obtain three result of detections.
For example, three result of detections constitute one group of ternary once linear equation group, according to the system of linear equations, separation
Go out the fundamental component and the positive and negative frequency-shifted components of image signal.
For example, the fundamental component and the positive and negative frequency-shifted components are combined in spatial frequency domain, and conversion is made the return trip empty
Between position field, to recover the image of the target object.
For example, the spatial light modulator includes：Digital micromirror array or liquid crystal modulator.
In the embodiment of the present invention, imaging system is divided different wave length using grating by the way that pulsed light is carried out into spectrum segmentation
The pulsed light of amount is separated in space, is radiated at digital micromirror array and is loaded with the position of different candy strips, realizes structure
The parallel acquisition of light.The process is not related to the process an electronically or mechanically moved, and substantially increases one vertical frame dimension resolution image institute of reconstruct
The time needed, finally realize the structure light super-resolution imaging system of a high speed.
Brief description of the drawings
In order to illustrate the technical solution of the embodiments of the present invention more clearly, making below by required in the description to embodiment
Accompanying drawing is briefly described.Drawings in the following description are only the exemplary embodiment of the present invention.
Fig. 1 shows a kind of structural representation of high-speed structures photoimaging systems according to embodiments of the present invention；
Fig. 2 shows the structural representation of another high-speed structures photoimaging systems according to embodiments of the present invention.
Embodiment
Hereinafter, by preferred embodiments of the present invention will be described in detail with reference to the annexed drawings.Note, in the specification and drawings
In, it is denoted by the same reference numerals with substantially the same step and element, and to these steps and the repetition solution of element
Releasing to be omitted.
Fig. 1 describes the structural representation of high-speed structures photoimaging systems 100 according to an embodiment of the invention.Under
Face describes reference picture 1 the high-speed structures photoimaging systems of one embodiment of the present of invention.Referring to Fig. 1, high-speed structures light into
As system 100 includes：Pulse optical signal generating device 110, optical circulator 120, spatial dispersion device 130, spatial light modulator
140 and photoimaging equipment 150.Pulse optical signal generating device 110 is used to produce pulsed optical signals.Optical circulator 120 is used for
Light path is adjusted, so that the pulsed optical signals for inciding optical circulator 120 from pulse optical signal generating device 110 enter the first light
Road, then returned from the first light path, and incide photoimaging equipment 150.Spatial dispersion device 130 (such as grating or prism), position
In the first light path, for by from the frequency spectrum of the incident pulsed optical signals of optical circulator 120 in space development.Spatial light modulator
140, it also is located in the first light path, for will be modulated in the optical signal of space development.The utilization space light of photoimaging equipment 150
Optical signal after modulator 140 is modulated is imaged to target object.
According to the example of the present invention, pulse optical signal generating device 110 can be wide range light-pulse generator, short light pulse
Source or DC light source.For example, being in the wide range light-pulse generator of visible light wave range (400~760nm) using centre wavelength.
According to the example of the present invention, spatial dispersion device 130 is grating, such as diffraction grating or scattered grating.By
Acted in by Grating angular spectral dispersion, the angle of diffraction of the light of different wave length is different, therefore wavelength component different in pulsed light exists
Spatially scatter.For example, spatial dispersion device 130 can receive the incident light of optical circulator 120 by a speculum.
According to the example of the present invention, spatial light modulator 140 can be modulated to the direction in space of light.In addition,
Spatial light modulator can also be modulated to the phase of light.Or, spatial light modulator can also to the direction in space of light and
Phase is modulated simultaneously.
Spatial light modulator can by light modulation to three different direction in spaces, or, spatial light modulator can be with
By the spatial frequency modulation of light into three different phases.In addition, spatial light modulator can also be to the spatial frequency amplitude of light
It is modulated.
For example, that spatial light modulator is digital micromirror array (DMD), for carrying out intensity modulated to light.Will with DMD
The intensity distribution of hot spot is modulated into the striated of Sine distribution.Here can by digital programmable change striped hot spot direction and
Initial phase.Three groups of striped hot spots at such as 60 degree of stripe direction interval, 0 degree is set to by the direction of one of striped hot spot,
Then the direction of another two striped hot spot is 60 degree and 120 degree.
Fig. 2 shows the structural representation of another high-speed structures photoimaging systems according to embodiments of the present invention.Referring to
Fig. 2, according to the example of the present invention, high-speed structures photoimaging systems 100 can also include wave filter 160, and wave filter is used for
Wavelength of the light based on light that spatial dispersion device deploys is filtered, to be divided into different-waveband.So, space light modulation
The light for the different-waveband that device is divided into based on wave band to wave filter is modulated.For example, spatial light modulator is by the light of different-waveband
It is modulated on three different direction in spaces.Or, spatial light modulator is by the light modulation of different-waveband into three different phases
Position.
In order to which by the light modulation of different-waveband, into three different phases and direction, spatial light modulator is digital micro-mirror battle array
Row, by modulating direction and the angle of digital micromirror array, come the direction of the light of modulating injection and phase.For example, digital micro-mirror
Array modulation is different pattern, so, incides the space of the light of digital micromirror array and then has different direction in spaces respectively
And phase.The pattern can be striped or square, other patterns such as dot matrix.When pattern is striped, striped can be wrapped
Include at least two.Alternatively, in order to improve image resolution ratio, striped can include three kinds of stripeds or more than three kinds stripeds.
Alternatively, at least two stripeds include at least two in horizontal stripe, nicking and inclined stripe.Certainly, if resolution ratio will
Ask not high, it would however also be possible to employ one kind in horizontal stripe, nicking and inclined stripe.Referring to Fig. 2, Fig. 2 shows space light modulation
Candy strip P and illumination that device is modulated are mapped to the circular light spot L produced on candy strip.
For example, wave filter 160 selects the pulsed light of wherein 9 wavelength components, according to the principle of structure light super-resolution imaging,
In order to obtain high-resolution image, a vertical frame dimension resolution image can be loaded with different directions, out of phase striped by 9 frames
Low resolution image is rebuild.Such as three 0 degree, 60 degree and 120 degree of directions, each direction is again comprising 3 phases.These pulsed lights
The diverse location on the digital micromirror array as spatial light modulator is radiated at, by advance entering micro mirror array relevant position
The coding of the above-mentioned 9 kinds of stripeds of row, we can be implemented without automatically controlled or mechanical movement mode and meanwhile obtain 9 kinds it is different
Structure light.
According to the present invention an example, spatial light modulator can the different luminous intensities based on incident light, by different ripples
The light modulation of section is on different pattern.Replaceable or increase ground, spatial light modulator can also be based on the color do not shared the same light, will not
Light modulation with wave band is into different pattern.
According to the example of the present invention, spatial light modulator 140 can be digital micromirror array or liquid crystal modulator.This
In we use digital micromirror array or liquid crystal modulator the reason for be can flexible modulation striped width and interval, when modulation ginseng
After number is determined, fixed pattern mask plate can be used to replace digital micromirror array, by the pattern of fixed pattern mask plate, to adjust
Make direction and the phase for the light injected.So as to effectively save cost.
According to the example of the present invention, high-speed structures photoimaging systems 100 can also include lens 170, and lens 170 can
So that between wave filter 160 and spatial light modulator 140, lens 170 can be converged the light of filters filter, this
Sample, spatial light modulator is modulated to the light of the different-waveband after convergence.
According to the example of the present invention, photoimaging equipment 150 can include object lens, and object lens are utilized to be returned from the first light path
Modulation after light target object is imaged.For example, with reference to Fig. 2, from the emergent light of optical circulator 120 incides object lens.
For example, object lens include the first object lens 151 and the second object lens 152, respectively positioned at target object S both sides.First object lens 151 connect
Receive the light after the modulation returned from the first light path and incide target object, the second object lens 152 to the reflected light of target object and/
Or scattered light is acquired.By using the first object lens 151 and the second object lens 152, target object can be in objective lens numerical
Spatial spectral information outside aperture is moved within numerical aperture.
According to the example of the present invention, photoimaging equipment 150 also includes the second Dispersive Devices 153 (such as grating), uses
Dispersion is carried out in the light gathered to the second object lens 152, the light that target object reflects and/or scatters is separated in space, irradiation
Onto the different spatial of charge coupled device ccd.Photoimaging equipment 150 can also include the second lens 154, the second lens
The light that 154 pair of second Dispersive Devices 153 is gathered is converged.
According to the example of the present invention, photoimaging equipment 150 also includes photo-sensitive cell 155, such as charge coupling device
CCD, for being imaged to the light gathered from target object.As shown in Fig. 2 by photoimaging equipment 150, can obtain to mesh
Object S image is marked, the image includes three patterns modulated with spatial light modulator 140, it is possible to use this 3
Image recovers a high-resolution image.
Due to the light using sinusoidal intensity carry out it is incident, therefore each image result of detection be all fundamental frequency signal with it is positive and negative
The linear combination of two sinusoidal frequency-shifted components, the wherein linear coefficient of frequency-shifted components have with modulating the initial phase of sinusoidal pattern
Close.The measurement result of three different first phases, may be constructed one group of ternary once linear equation group, so as to isolate picture signal base
Frequency component and two positive and negative frequency-shifted components containing high-frequency information.Then, three is combined in spatial frequency domain, and switched back to
Locus domain can recover the enhanced image of resolution ratio.
To sum up, innovative point of the invention is the imaging system by the way that wide range pulsed light is carried out into spectrum segmentation, using spreading out
Penetrate grating to separate the pulsed light of different wave length component in space, be radiated at the position that micro mirror array is loaded with different candy strips
On, realize the parallel acquisition of structure light.The process is not related to the process an electronically or mechanically moved, and substantially increases one vertical frame dimension of reconstruct
Time needed for resolution image, finally realize the structure light super-resolution imaging system of a high speed.
Those of ordinary skill in the art are it is to be appreciated that the list of each example described with reference to the embodiments described herein
Member and algorithm steps, can be realized with electronic hardware, computer software or the combination of the two.And software module can be put
In any form of computer-readable storage medium.In order to clearly demonstrate the interchangeability of hardware and software, in the above description
The composition and step of each example have been generally described according to function.These functions are come with hardware or software mode actually
Perform, depending on the application-specific and design constraint of technical scheme.Those skilled in the art can specifically should to each
For realizing described function using distinct methods, but this realization is it is not considered that beyond the scope of this invention.
It should be appreciated by those skilled in the art that can be dependent on design requirement and other factorses carries out various repair to the present invention
Change, combine, part is combined and replaced, as long as they are in the range of appended claims and its equivalent.
Claims (10)
1. a kind of high-speed structures photoimaging systems, including：Pulse optical signal generating device, optical circulator, spatial dispersion device is empty
Between optical modulator and photoimaging equipment；
The pulse optical signal generating device, for producing pulsed optical signals；
The optical circulator, for adjusting light path, so as to believe from the incident pulsed light of the pulse optical signal generating device
Number enter the first light path through the optical circulator, then returned from first light path, and incide the photoimaging equipment；
Spatial dispersion device, in first light path, for by from the frequency of the incident pulsed optical signals of the optical circulator
Spectrum is in space development；
Spatial light modulator, in first light path, for the optical signal in space development to be modulated；
Photoimaging equipment, the optical signal after being modulated using the spatial light modulator is imaged to target object.
2. photoimaging systems according to claim 1, wherein, the pulse optical signal generating device is wide range pulsed light
Source, short-pulse light source or DC light source.
3. photoimaging systems according to claim 1, wherein, the spatial light modulator enters to the direction in space of the light
Row modulation.
4. the photoimaging systems according to claim 1 or 3, wherein, the spatial light modulator enters to the phase of the light
Row modulation.
5. photoimaging systems according to claim 1, wherein, the spatial light modulator enters to the spatial frequency amplitude of light
Row modulation.
6. the photoimaging systems according to claim 3 or 4, in addition to wave filter,
The wave filter is used to wavelength of the light based on light that the spatial dispersion device deploys being filtered, to be divided into not
Same wave band；
The light for the different-waveband that the spatial light modulator is divided into based on wave band to the wave filter is modulated.
7. photoimaging systems according to claim 6, wherein, the spatial light modulator adjusts the light of the different-waveband
Make onto three different direction in spaces.
8. the photoimaging systems according to claim 6 or 7, wherein, the spatial light modulator is by the different-waveband
The spatial frequency modulation of light is into three different phases.
9. the photoimaging systems according to claim 3 or 4, wherein, the spatial light modulator is digital micromirror array, is led to
The direction of digital micromirror array described in ovennodulation and angle, come the direction of the light of modulating the injection and phase.
10. the photoimaging systems according to claim 3 or 4, wherein, the spatial light modulator is fixed pattern mask
Version, based on the pattern of the fixed pattern mask plate, come the direction of the light of modulating the injection and phase.
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| CN108895982A (en) * | 2018-07-03 | 2018-11-27 | 清华大学 | High-speed structures light generating device and three-dimensional single pixel imaging system |
| CN110244446A (en) * | 2019-07-11 | 2019-09-17 | 中国科学院广州生物医药与健康研究院 | A kind of super-resolution microscope |
| CN110809102A (en) * | 2019-10-11 | 2020-02-18 | 北京理工大学 | Imaging acceleration method and device based on binary modulation |
| CN111698435A (en) * | 2020-06-10 | 2020-09-22 | 北京理工大学 | Space-frequency spectrum multi-dimensional joint modulation imaging acceleration method and device |
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| CN111698435A (en) * | 2020-06-10 | 2020-09-22 | 北京理工大学 | Space-frequency spectrum multi-dimensional joint modulation imaging acceleration method and device |
| CN111698435B (en) * | 2020-06-10 | 2021-04-27 | 北京理工大学 | Space-frequency spectrum multi-dimensional joint modulation imaging acceleration method and device |
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