CN107271524A - MFC biology sensor of the one kind based on (CNTs/PANI) n ito anodes is used for the method for drug sensitive test - Google Patents
MFC biology sensor of the one kind based on (CNTs/PANI) n ito anodes is used for the method for drug sensitive test Download PDFInfo
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- CN107271524A CN107271524A CN201710513917.5A CN201710513917A CN107271524A CN 107271524 A CN107271524 A CN 107271524A CN 201710513917 A CN201710513917 A CN 201710513917A CN 107271524 A CN107271524 A CN 107271524A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/403—Cells and electrode assemblies
- G01N27/413—Concentration cells using liquid electrolytes measuring currents or voltages in voltaic cells
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M8/00—Fuel cells; Manufacture thereof
- H01M8/16—Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/50—Fuel cells
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P70/00—Climate change mitigation technologies in the production process for final industrial or consumer products
- Y02P70/50—Manufacturing or production processes characterised by the final manufactured product
Abstract
It is used for the method for drug sensitive test the invention discloses a kind of MFC biology sensors based on (CNTs/PANI) n ito anodes, carbon nano-tube/poly aniline compound modification ito anode is prepared using LBL self-assembly method, for building single chamber and double-chamber microbiological fuel cell biology sensor, and it is applied to the drug sensitive test of the medicines such as gentamicin.The present invention proposes a kind of new method to traditional antibiotic drug sensitive test method, the problems such as avoiding microorganism flat band method detection time length, cumbersome, poor repeatability, constructed microbiological fuel cell biology sensor is easy to operate, and can realize quick, real-time, highly sensitive drug sensitive test.
Description
Technical field
The invention belongs to the technical field that microbiological fuel cell biology sensor detects medicine, and in particular to one kind is based on
(CNTs/PANI) the MFC biology sensors of n-ITO anodes are used for the method for drug sensitive test.
Background technology
The abuse of antibiotic seriously threatens the life and health of the mankind in recent years.Traditional antibiotic drug sensitive test method(s) bag
Include disk diffusion method, agar dilution, E test method(s)s and broth dilution method etc..However, these methods all exist it is time-consuming longer
(24~48h) and cumbersome deficiency, it is impossible to the need for meeting clinical quick diagnosis treatment.Microbiological fuel cell (MFC)
It is, using microorganism as catalyst, chemical energy directly to be turned to biology sensor as a kind of device for turning to electric energy, is used for electricity production
The fields such as energy supply, sewage disposal, environmental organism reparation and biology sensor.Microbiological fuel cell is used for field of biosensors
It can be used to detect noxious material.General principle is that noxious material enters after MFC, and the metabolism of electro-chemical activity bacterium is by noxious material
Suppression, cause output current to reduce, and the decline degree of electric current and the concentration of noxious material have certain correlation.It is poisonous
Substance toxicity is bigger, and current reduction amplitude is bigger, and difference can be built according to the relation between noxious material and current reduction amplitude
Toxic sensors.Although constructing biology sensor using MFC at present to detect for antibiotic, not to passing
The detection sensitivity of sensor and the minimum detection limit of antibiotic are studied.
The sensing characteristicses of biology sensor based on microbiological fuel cell depend primarily on the production of microbiological fuel cell
Electrical property.Anode material is because direct and microorganism contacts and transmits extracellular electronics as one of important influence factor.Nanogold
The electrode materials such as metal particles, CNT, nano structural conductive polymer are applied to that in microbiological fuel cell production can be improved
Electrical property.Although current people have attempted distinct methods and have been prepared for MFC nano structural material anodes, there is not preparation nano junction
Structure carbon nano-tube/poly aniline compound modifies ito anode and for building MFC sensors, carries out drug sensitive test research, solves medicine
The problem of quick assay sensitivity.
The content of the invention
It is an object of the invention to overcome the deficiencies in the prior art part there is provided one kind based on (CNTs/PANI) n-ITO
The MFC biology sensors of anode are used for the method for drug sensitive test, and preparing carbon nano-tube/poly aniline using LBL self-assembly method answers
Compound modification ito anode (i.e. (CNTs/PANI) n-ITO anodes), builds microbiological fuel cell (MFC) biology sensor, and
It is applied to gentamicin drug sensitive test.The present invention proposes a kind of new method to traditional antibiotic drug sensitive test method,
The problems such as avoiding microorganism flat band method detection time length, cumbersome, poor repeatability, constructed microbiological fuel cell life
Thing sensor operations are easy, and can realize quick, real-time, highly sensitive drug sensitive test.
The technical solution adopted for the present invention to solve the technical problems is:
Microbiological fuel cell biology sensor of the one kind based on (CNTs/PANI) n-ITO anodes is used for drug sensitive test
Method, including:
1) ITO (tin indium oxide) electrode after being handled through 3~5%NaOH solution is immersed in anhydrous toluene solution, added
APTES (3- aminopropyl triethoxysilanes), the formula rate of wherein APTES and dry toluene is 1.0~1.2g:25mL;
In nitrogen atmosphere after 15~17h of room temperature, ITO electrode is taken out, is cleaned by ultrasonic successively with toluene, ethanol and deionized water, most
Nitrogen is dried up afterwards;
2) volume ratio 1 is used:2.5~3.5 saturation concentrated nitric acid and the mixed solution of the concentrated sulfuric acid enter to CNTs (CNT)
23~25h of row ultrasound acidifications, centrifugation, with milli-Q water, is centrifuged repeatedly washing untill supernatant is in neutrality;Will be from
The lyophilized preservations of CNTs that gains in depth of comprehension are arrived, are then configured to 0.9~1.1mg/mL CNTs suspension with ultra-pure water;
3) by mass ratio 1:0.9~1.1 PANI (polyaniline) and HCSA (camphorsulfonic acid) is dissolved into chloroform, and stirring is extremely
Solvent volatilization is complete, then uses ultra-pure water ultrasonic disperse, and the PANI for being configured to the HCSA doping that concentration is 0.9~1.1mg/mL is molten
Liquid;
4) by step 1) obtained ITO electrode is soaked in step 3) 25 in the obtained PANI solution of HCSA doping~
35min, takes out and uses ultra-pure water 4~6min of soaking and washing, then be soaked in step 2) 25~35min in obtained CNTs suspension,
Ultra-pure water 4~6min of soaking and washing is taken out, (CNTs/PANI) n-ITO electrodes are obtained, wherein n is the modification number of plies, n=1;By institute
(CNTs/PANI) the n-ITO electrodes for stating n=1 repeat the above steps, and obtain (CNTs/PANI) n-ITO electricity of the different modifying number of plies
Pole;
5) (CNTs/PANI) n-ITO electrodes described in utilizing build single-chamber microbial fuel cell biology sensor:With described
(CNTs/PANI) n-ITO electrodes are working electrode, and Pt is that, to electrode, saturation Ag/AgCl electrodes are reference electrode, and single chamber is biological
Added in sensor after the electrolyte containing medicine, 0.18~0.22V of constant potential, 36~38 DEG C of 25~35min of operation of constant temperature, plus
Enter bacterium solution, the electrochemical parameter of observation single-chamber microbial fuel cell biology sensor changes to carry out drug sensitive test;And/or,
Double-chamber microbiological fuel cell biology sensor is built using (CNTs/PANI) the n-ITO electrodes:With described
(CNTs/PANI) n-ITO electrodes are anode, and carbon cloth is negative electrode, and anode chamber adds electrolyte and bacterium solution, cathode chamber adds 9~
After 11mM potassium ferricyanide solutions, the Ω of extrernal resistance 1900~2100,36~38 DEG C of 24~26h of operation of constant temperature, medicine is added, dual chamber is observed
The electrochemical parameter of microbiological fuel cell biology sensor changes to carry out drug sensitive test.
In one embodiment:The n=1,3,6,8,12,15.
In one embodiment:The n=12.
In one embodiment:The bacterium of the bacterium solution is that can produce the pathogenic microorganism of extracellular electronics.
In one embodiment:The bacterium of the bacterium solution is Escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, epidermis Portugal
At least one of grape coccus, streptococcus pneumonia, klebsiella.
In one embodiment:The bacterium of the bacterium solution is Shewanella loihica PV-4 and/or Escherichia coli
25922。
In one embodiment:The medicine grows and activity for that can kill pathogenic microorganism and/or can suppress pathogenic microorganism
Medicine.
In one embodiment:The medicine is antibiotic.
In one embodiment:The medicine is penicillins, cephalosporins, aminoglycoside, macrolides, quinolone
At least one of class antibiotic.
In one embodiment:The medicine is gentamicin.
The technical program is compared with background technology, and it has the following advantages that:
The present invention builds biology sensor using microbiological fuel cell, for gentamicin drug sensitive test research, it is to avoid
Traditional technique in measuring time length and cumbersome deficiency.The present invention is prepared for carbon nano-tube/poly aniline compound modification ITO
Anode simultaneously uses it for microbiological fuel cell biology sensor, further increases the detection sensitivity of sensor, and in fact
Real time on-line monitoring is showed.
Brief description of the drawings
The invention will be further described with reference to the accompanying drawings and examples.
Fig. 1 be embodiment 1 prepare the different number of plies CNTs/PANI modification ITO electrode in microbiological fuel cell when
M- current curve.
Fig. 2 is that different number of plies CNTs/PANI modification ITO electrodes prepared by embodiment 1 adhere to shadow to S.loihica PV-4
In loud SEM figures, figure, (a) modifies 1 layer, and (b) modifies 3 layers, and (c) modifies 6 layers, and (d) modifies 8 layers, and (e) modifies 12 layers, and (f) is repaiied
15 layers of decorations.
Under the conditions of Fig. 3 is addition various concentrations gentamicin in embodiment 2, based on (CNTs/PANI)12The list of-ito anode
The current-responsive curve of chamber microbiological fuel cell biology sensor.
Under the conditions of Fig. 4 is addition various concentrations gentamicin in embodiment 3, based on (CNTs/PANI)12Pair of-ito anode
The voltage response curves of chamber microbiological fuel cell biology sensor.
Embodiment
Present disclosure is illustrated below by embodiment:
Embodiment 1
1) ITO (tin indium oxide) electrode after being handled through 4%NaOH solution is immersed in 25mL anhydrous toluene solutions, added
About 1.1g APTES (3- aminopropyl triethoxysilanes), in nitrogen atmosphere after room temperature 16h, takes out ITO electrode,
It is cleaned by ultrasonic successively with toluene, ethanol and deionized water, last nitrogen drying;
2) with saturation concentrated nitric acid and mixed solution (v/v, 1 of the concentrated sulfuric acid:3) 24h ultrasounds are carried out to CNTs (CNT)
Acidification, centrifugation, with milli-Q water untill supernatant is in neutrality;The obtained lyophilized preservations of CNTs will be centrifuged, then
1mg/mL CNTs suspension is configured to ultra-pure water;
3) 100mg PANI (polyaniline) and 100mg HCSA (camphorsulfonic acid) is dissolved into 20mL chloroforms, stirring is extremely
Solvent volatilization is complete, then uses ultra-pure water ultrasonic disperse, is configured to the PANI solution that the HCSA that concentration is 1mg/mL adulterates;
4) by step 1) obtained ITO electrode is soaked in step 3) 30min in the obtained PANI solution of HCSA doping, take
Go out to use ultra-pure water soaking and washing 5min, then be soaked in step 2) 30min in obtained CNTs suspension, takes out ultra-pure water immersion clear
5min is washed, (CNTs/PANI) n-ITO electrodes, wherein n=1 is obtained;(CNTs/PANI) the n-ITO electrodes of the n=1 are repeated
Above-mentioned steps, obtain (CNTs/PANI) the n-ITO electrodes (n=1,3,6,8,12,15) of the different modifying number of plies;
Using (CNTs/PANI) n-ITO electrodes as working electrode, Pt is that, to electrode, saturation Ag/AgCl electrodes are reference electricity
Electrolyte is added in pole, reactor, constant potential is 0.2V, and 37 DEG C of constant temperature is run after 30min, adds OD600For 2.0
Shewanella loihica PV-4 bacterium solutions, carry out electricity production experiment.
As a result as depicted in figs. 1 and 2, Fig. 1 is that different number of plies CNTs/PANI modify ITO electrode in microbiological fuel cell
In time current curve, as seen from the figure, modification number of plies n=12 (CNTs/PANI)12The current density that-ITO electrode is produced
It is worth maximum (6.98 μ A/cm2).Fig. 2 is that different number of plies CNTs/PANI modify ITO electrode to S.loihica PV-4 adhesion influences
SEM figures, as seen from the figure, S.loihica PV-4 adhesivenesses on more than 3 layers of electrode of modification are preferable.
Embodiment 2
1) ITO electrode after being handled through 4%NaOH solution is immersed in 25mL anhydrous toluene solutions, it is about 1.1g's to add
APTES, in nitrogen atmosphere after room temperature 16h, takes out ITO electrode, clear with toluene, ethanol and deionized water ultrasound successively
Wash, last nitrogen drying;
2) with saturation concentrated nitric acid and mixed solution (v/v, 1 of the concentrated sulfuric acid:3) the ultrasonic acidifications of 24h are carried out to CNTs, from
The heart, with milli-Q water untill supernatant is in neutrality;The obtained lyophilized preservations of CNTs will be centrifuged, then prepared with ultra-pure water
Into 1mg/mL CNTs suspension;
3) 100mg PANI and 100mg HCSA is dissolved into 20mL chloroforms, stirring is complete to solvent volatilization, then
Ultra-pure water ultrasonic disperse is used, the PANI solution that the HCSA that concentration is 1mg/mL adulterates is configured to;
4) by step 1) obtained ITO electrode is soaked in step 3) 30min in the obtained PANI solution of HCSA doping, take
Go out to use ultra-pure water soaking and washing 5min, then be soaked in step 2) 30min in obtained CNTs suspension, takes out ultra-pure water immersion clear
Wash 5min;Repeat the above steps to common and carry out 12 times, (CNTs/PANI) for obtaining modifying number of plies n=1212- ITO electrode;
5) described in utilizing (CNTs/PANI)12- ITO electrode builds single-chamber microbial fuel cell biology sensor, and carries out
Drug sensitive test:With (CNTs/PANI)12- ITO electrode is working electrode, and Pt is that, to electrode, saturation Ag/AgCl electrodes are ginseng
Than electrode, the electricity for the gentamicin that concentration is 0mM, 0.25mM, 0.5mM, 1mM, 1.5mM is separately added into single chamber biology sensor
Solve after liquid, constant potential 0.2V, 37 DEG C of operation 30min of constant temperature, add OD600For 2.0 bacterium of Escherichia coli 25922
Liquid, carries out electricity production experiment, and the electrochemical parameter of observation single-chamber microbial fuel cell biology sensor changes to carry out susceptibility examination
Test.
Under the conditions of Fig. 3 is addition various concentrations gentamicin, based on (CNTs/PANI)12The single-chamber microbial of-ito anode
The current-responsive curve of fuel cell biology sensor.As seen from the figure, with the increase of gentamicin concentration, the electric current of sensor
Signal is gradually reduced, and the lowest detection of gentamicin is limited to 0.25mM.
Embodiment 3
1) ITO electrode after being handled through 4%NaOH solution is immersed in 25mL anhydrous toluene solutions, it is about 1.1g's to add
APTES, in nitrogen atmosphere after room temperature 16h, takes out ITO electrode, clear with toluene, ethanol and deionized water ultrasound successively
Wash, last nitrogen drying;
2) with saturation concentrated nitric acid and mixed solution (v/v, 1 of the concentrated sulfuric acid:3) the ultrasonic acidifications of 24h are carried out to CNTs, from
The heart, with milli-Q water untill supernatant is in neutrality;The obtained lyophilized preservations of CNTs will be centrifuged, then prepared with ultra-pure water
Into 1mg/mL CNTs suspension;
3) 100mg PANI and 100mg HCSA is dissolved into 20mL chloroforms, stirring is complete to solvent volatilization, then
Ultra-pure water ultrasonic disperse is used, the PANI solution that the HCSA that concentration is 1mg/mL adulterates is configured to;
4) by step 1) obtained ITO electrode is soaked in step 3) 30min in the obtained PANI solution of HCSA doping, take
Go out to use ultra-pure water soaking and washing 5min, then be soaked in step 2) 30min in obtained CNTs suspension, takes out ultra-pure water immersion clear
Wash 5min;Repeat the above steps to common and carry out 12 times, (CNTs/PANI) for obtaining modifying number of plies n=1212- ITO electrode;
5) described in utilizing (CNTs/PANI)12- ITO electrode builds double-chamber microbiological fuel cell biology sensor, and carries out
Drug sensitive test:With (CNTs/PANI)12- ITO electrode is anode, and carbon cloth is negative electrode, and anode chamber adds electrolyte and OD600For
2.0 bacterium solutions of Escherichia coli 25922, cathode chamber adds 10mM potassium ferricyanide solutions, connects 2000 Ω extrernal resistances, permanent
After 37 DEG C of operation 25h of temperature, the gentamicin that concentration is 0mM, 0.25mM, 0.5mM, 1mM, 1.5mM is separately added into, electricity production is carried out real
Test, the electrochemical parameter of observation double-chamber microbiological fuel cell biology sensor changes to carry out drug sensitive test.
Under the conditions of Fig. 4 is addition various concentrations gentamicin, based on (CNTs/PANI)12The double-chamber microbiological of-ito anode
The voltage response curves of fuel cell biology sensor.As seen from the figure, voltage constantly rises from 0mV after inoculation bacterium solution, after 8h about
150mV is then stable in 120mV or so.Run after 25h, decline with the addition voltage of gentamicin.Gentamicin concentration is got over
Height, the voltage signal decline of sensor is about notable, and the lowest detection of gentamicin is limited to 0.25mM, with single-chamber microbial fuel electricity
The gentamicin drug sensitive test result of pond biology sensor has good uniformity.
It is described above, only present pre-ferred embodiments, therefore the scope that the present invention is implemented can not be limited according to this, i.e., according to
The equivalent changes and modifications that the scope of the claims of the present invention and description are made, all should still belong in the range of the present invention covers.
Claims (10)
1. microbiological fuel cell biology sensor of the one kind based on (CNTs/PANI) n-ITO anodes is used for the side of drug sensitive test
Method, it is characterised in that:Including:
1) ITO electrode after being handled through 3~5%NaOH solution is immersed in anhydrous toluene solution, adds APTES, wherein APTES
Formula rate with dry toluene is 1.0~1.2g:25mL;In nitrogen atmosphere after 15~17h of room temperature, ITO electricity is taken out
Pole, is cleaned by ultrasonic with toluene, ethanol and deionized water successively, last nitrogen drying;
2) volume ratio 1 is used:2.5~3.5 saturation concentrated nitric acid and the mixed solution of the concentrated sulfuric acid carry out 23~25h ultrasound acid to CNTs
Change is handled, centrifugation, with milli-Q water untill supernatant is in neutrality;The obtained lyophilized preservations of CNTs, Ran Houyong will be centrifuged
Ultra-pure water is configured to 0.9~1.1mg/mL CNTs suspension;
3) by mass ratio 1:0.9~1.1 PANI and HCSA is dissolved into chloroform, and stirring is complete to solvent volatilization, then with super
Pure water ultrasonic disperse, is configured to the PANI solution that the HCSA that concentration is 0.9~1.1mg/mL adulterates;
4) by step 1) obtained ITO electrode is soaked in step 3) 25~35min in the obtained PANI solution of HCSA doping, take
Go out to use ultra-pure water 4~6min of soaking and washing, then be soaked in step 2) 25~35min in obtained CNTs suspension, takes out ultrapure
Water 4~6min of soaking and washing, obtains (CNTs/PANI) n-ITO electrodes, wherein n=1;By (CNTs/PANI) n- of the n=1
ITO electrode repeats the above steps, and obtains (CNTs/PANI) n-ITO electrodes of the different modifying number of plies;
5) (CNTs/PANI) n-ITO electrodes described in utilizing build single-chamber microbial fuel cell biology sensor:With described
(CNTs/PANI) n-ITO electrodes are working electrode, and Pt is that, to electrode, saturation Ag/AgCl electrodes are reference electrode, and single chamber is biological
Added in sensor after the electrolyte containing medicine, 0.18~0.22V of constant potential, 36~38 DEG C of 25~35min of operation of constant temperature, plus
Enter bacterium solution, the electrochemical parameter of observation single-chamber microbial fuel cell biology sensor changes to carry out drug sensitive test;And/or,
Double-chamber microbiological fuel cell biology sensor is built using (CNTs/PANI) the n-ITO electrodes:With (the CNTs/
PANI) n-ITO electrodes are anode, and carbon cloth is negative electrode, and anode chamber adds electrolyte and bacterium solution, and cathode chamber adds 9~11mM iron cyanogen
Change after potassium solution, the Ω of extrernal resistance 1900~2100,36~38 DEG C of 24~26h of operation of constant temperature, add medicine, observation double-chamber microbiological combustion
The electrochemical parameter of material battery biology sensor changes to carry out drug sensitive test.
2. the microbiological fuel cell biology sensor according to claim 1 based on (CNTs/PANI) n-ITO anodes is used
In the method for drug sensitive test, it is characterised in that:The n=1,3,6,8,12,15.
3. the microbiological fuel cell biology sensor according to claim 1 based on (CNTs/PANI) n-ITO anodes is used
In the method for drug sensitive test, it is characterised in that:The n=12.
4. the microbiological fuel cell biology sensor according to claim 1 based on (CNTs/PANI) n-ITO anodes is used
In the method for drug sensitive test, it is characterised in that:The bacterium of the bacterium solution is that can produce the pathogenic microorganism of extracellular electronics.
5. the microbiological fuel cell biology sensor according to claim 1 based on (CNTs/PANI) n-ITO anodes is used
In the method for drug sensitive test, it is characterised in that:The bacterium of the bacterium solution is Escherichia coli, staphylococcus aureus, P. aeruginosa
At least one of bacterium, MRSE, streptococcus pneumonia, klebsiella.
6. the microbiological fuel cell biology sensor according to claim 1 based on (CNTs/PANI) n-ITO anodes is used
In the method for drug sensitive test, it is characterised in that:The bacterium of the bacterium solution be Shewanella loihica PV-4 and/or
Escherichia coli 25922。
7. the microbiological fuel cell biology sensor according to claim 1 based on (CNTs/PANI) n-ITO anodes is used
In the method for drug sensitive test, it is characterised in that:The medicine is given birth to for that can kill pathogenic microorganism and/or can suppress pathogenic microorganism
Long and activity medicine.
8. the microbiological fuel cell biology sensor according to claim 1 based on (CNTs/PANI) n-ITO anodes is used
In the method for drug sensitive test, it is characterised in that:The medicine is antibiotic.
9. the microbiological fuel cell biology sensor according to claim 1 based on (CNTs/PANI) n-ITO anodes is used
In the method for drug sensitive test, it is characterised in that:The medicine is penicillins, cephalosporins, aminoglycoside, macrolide
At least one of class, carbostyril antibiotic.
10. the microbiological fuel cell biology sensor according to claim 1 based on (CNTs/PANI) n-ITO anodes
Method for drug sensitive test, it is characterised in that:The medicine is gentamicin.
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