CN107271419A - A kind of gel-colored scanned imagery device and its dyeing imaging method - Google Patents
A kind of gel-colored scanned imagery device and its dyeing imaging method Download PDFInfo
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- CN107271419A CN107271419A CN201710605700.7A CN201710605700A CN107271419A CN 107271419 A CN107271419 A CN 107271419A CN 201710605700 A CN201710605700 A CN 201710605700A CN 107271419 A CN107271419 A CN 107271419A
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- gel
- sample cell
- colored
- closed
- flushing liquor
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- 238000003384 imaging method Methods 0.000 title claims abstract description 35
- 238000004043 dyeing Methods 0.000 title claims abstract description 11
- 238000011010 flushing procedure Methods 0.000 claims abstract description 54
- 230000033001 locomotion Effects 0.000 claims abstract description 29
- 238000000034 method Methods 0.000 claims abstract description 12
- 230000008569 process Effects 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims description 16
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 claims description 8
- 229960005542 ethidium bromide Drugs 0.000 claims description 8
- 230000008859 change Effects 0.000 claims description 6
- 238000005192 partition Methods 0.000 claims description 6
- 238000011084 recovery Methods 0.000 claims description 5
- 239000000758 substrate Substances 0.000 claims description 3
- 239000000975 dye Substances 0.000 description 36
- 239000003292 glue Substances 0.000 description 5
- 230000003287 optical effect Effects 0.000 description 5
- 239000011159 matrix material Substances 0.000 description 4
- 230000011664 signaling Effects 0.000 description 4
- 239000006210 lotion Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 230000001627 detrimental effect Effects 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 238000004080 punching Methods 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 229920000297 Rayon Polymers 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000003471 mutagenic agent Substances 0.000 description 1
- 231100000707 mutagenic chemical Toxicity 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 238000010408 sweeping Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
Abstract
The present invention relates to a kind of gel-colored scanned imagery device and its dyeing imaging method, wherein gel-colored scanned imagery device includes gel-colored unit, UV scanning imaging unit, UV scanning imaging unit is arranged in closed scanning room, gel-colored unit is arranged in closed camera bellows, closed camera bellows, which is arranged in closed scanning room, forms one, and gel-colored, flushing, gel are reclaimed to be completed in closed camera bellows；Gel-colored unit includes sample cell, and sample cell bottom plate is uviol plate, and dye liquor box connects sample cell by dye liquor input and output pump, and flushing liquor box is connected by flushing liquor input and output pump and places gel piece on sample cell, sample cell bottom plate；UV scanning imaging unit includes motion scan ultraviolet source, motion scan CCD, fixed ultraviolet source.The present invention passes through the dyeing of the completion gel in closed integrated device, flushing, imaging and gel removal process, it is to avoid gel imaging analysis process pollution on the environment.
Description
First, technical field：
The present invention relates to the gel-colored and UV scanning imaging device of one kind, and in particular to be a kind of gel-colored scanning
Imaging device and its dyeing imaging method.
2nd, background technology：
The gel imaging system for being commonly used for obtaining gel images at present is a kind of dark box type photographing device, and its structure is
Camera bellows bottom is ultraviolet transmissive light source, and immediately top is immovable sample stage, the detected gel piece for placing.Sample
Platform be fixed in the middle of framework one piece can ultraviolet light thoroughly glass plate, uviol plate energy filtering clutter can only make a standing wave
Long UV light permeability.Gel piece is put using sample stage is pulled out during instrument, then is pushed back, passes through foreboard and the camera bellows fuselage of framework
Contact and stop bit.The charge coupling device set at the top of camera bellows（CCD）Take pictures and obtain the gel images that ultraviolet source is inspired, so
The gel images of acquisition are sent to computer again afterwards.
There are following two problems in this gel imaging system：1. it is camera bellows photo taking type, CCD due to obtaining gel images
Fluorescence signal distance is caught, when gel piece fluorescence signal is not strong, the image information of acquisition is difficult to meet analysis needs.2. coagulate
Blob of viscose need to be transported to sample stage progress image acquisition and gel recovery by manually operating from dyeing place, in this process people
Body, experimental operation table, instrument box etc. are all easily infected with and arrive gel piece, and the dyestuff that gel piece is used generally ethidium bromide
（EB）, ethidium bromide is strong mutagens, can be embedded in base molecule, cause mispairing, may have high carcinogenic.Gel contaminates
Color process easily causes experimental work environmental pollution and is detrimental to health, and has a strong impact on laboratory work safety.
Gel piece imaging is taken pictures using CCD catches imaging.Dot matrix CCD is used in DV and camera, i.e.,
Disposable instantaneous seizure two dimensional image optical signalling, the image required for being formed；Scanner uses linear CCD, using sweeping
Optical signalling is read in progressive scan when retouching element movement, ultimately forms two dimensional image.CCD chip is made up of many photosensitive units,
The optical signalling of seizure is converted into different electric charges by they, and the electric signal of formation is through simulation numeral（A/D）Conversion equipment is transmitted
To computer.CCD obtains the quality of image depending on the height of resolution ratio, and the height for obtaining image resolution ratio depends on photosensitive list
First quantity number, under the premise of identical photosensitive element number, the linear CCD used due to scanning is near and can obtain away from measured object
More fully sample optical signalling, shows the superiority of higher resolution.On the premise of identical image in different resolution is obtained,
Photosensitive unit required for linear CCD will be considerably less than dot matrix CCD, and be occupied little space relative to photo taking type dot matrix CCD.
3rd, the content of the invention：
It is an object of the present invention to provide a kind of gel-colored scanned imagery device, this gel-colored scanned imagery device is used
Experimental work environmental pollution is easily caused in the existing gel-colored process of gel imaging system of solution and is detrimental to health, seriously
Influence the problem of laboratory work is safe；It is a further object to provide the dye of this gel-colored scanned imagery device
Color imaging method.
The technical solution adopted for the present invention to solve the technical problems is：This gel-colored scanned imagery device includes solidifying
Glue dye unit, UV scanning imaging unit, UV scanning imaging unit are arranged in closed scanning room, gel-colored unit
It is arranged in closed camera bellows, closed camera bellows, which is arranged in closed scanning room, forms one, gel-colored, flushing, gel
Reclaim and completed in closed camera bellows；Gel-colored unit include sample cell, dye liquor box, flushing liquor box, dye liquor input and output pump,
Flushing liquor input and output pump, sample cell bottom plate is uviol plate, and dye liquor box connects sample cell, punching by dye liquor input and output pump
Washing lotion box is connected by flushing liquor input and output pump and places gel piece on sample cell, sample cell bottom plate；UV scanning imaging unit
Including motion scan ultraviolet source, motion scan CCD, fixed ultraviolet source, the scanning of motion scan ultraviolet source excites gel piece
Fluorescence signal is produced, motion scan CCD scannings catch gel fluorescence signal.
Closed camera bellows and closed scanning room are splits in such scheme, and one is formed by being fixed together, close
The camera bellows bottom closed has bottom plate hole, and sample cell bottom plate is fixed in bottom plate hole, has scanning holes at the top of scanning room, bottom plate hole with
Scanning holes is corresponding to be set；Dye liquor input and output pump, flushing liquor input and output pump connect sample cell by corresponding sebific duct
It is close to bottom of pond and is provided with the top of dye liquor access interface and flushing liquor access interface, sample cell set in bottom, sample cell pool wall bottom
It is equipped with sample cell cover plate.
Closed camera bellows and closed scanning room are integral in such scheme, by being set in closed lighttight casing
Put lateral partitions to be formed, bottom plate hole is set in lateral partitions, sample cell bottom plate is fixed in bottom plate hole；Dye liquor input and output pump,
Flushing liquor input and output pump connects the bottom of sample cell by corresponding sebific duct, and sample cell pool wall bottom is close to bottom of pond and is provided with
Sample cell cover plate is provided with the top of dye liquor access interface and flushing liquor access interface, sample cell.
Dye liquor liquid storage box is storage ethidium bromide in such scheme（EB）Or the plastic casing of other gel-colored liquid；Flushing liquor
Liquid storage box is the plastic casing of storage gel flushing liquor.
Motion scan ultraviolet source is that strip is portable in such scheme, and its wavelength matches 365 according to sample demand
Nanometer, 305 nanometers, 254 nanometers, any one in 420 nanometers.
Motion scan CCD is the color CCD device of linear array three in such scheme, 20 are arranged in by thousands of photosensitive units~
Constituted on the CCD substrates of 30mm length.
Ultraviolet source is fixed in such scheme for the ultraviolet transmissive light source needed for gel is reclaimed, its wavelength is according to sample
Demand matches any one in 365 nanometers, 305 nanometers, 254 nanometers, 20 nanometers.
The dyeing imaging method of above-mentioned gel-colored scanned imagery device：
First, completed in closed camera bellows gel-colored and rinse two processes：
A, gel piece is placed on sample cell bottom plate, starts dye liquor input and output pump and dye liquor is input to sample cell by dye liquor box,
Gel is dyed；
B, change dye liquor input and output pump motor direction of rotation, dye liquor box is recovered to by dye liquor；
C, startup flushing liquor input and output pump, flushing liquor are input to sample cell by flushing liquor box, gel are rinsed；
D, change flushing liquor input and output pump motor direction of rotation, flushing liquor box is recovered to by flushing liquor；
Then, start motion scan ultraviolet source, motion scan CCD, obtain gel images；
Finally, sample cell cover plate is opened, goggles is put on, fixed ultraviolet source is opened, carries out gel recovery.
Beneficial effect：
The present invention passes through the dyeing of the completion gel in closed integrated device, flushing, imaging and gel removal process, it is to avoid
Gel imaging analysis process pollution on the environment；Image mode, which is obtained, by the way that fixed dot matrix CCD is taken pictures is changed to portable cord
Property CCD scannings obtain image mode, shorten the distance of CCD and gel piece, reduce the decay of optical signal, improve and obtain image
Resolution ratio and picture quality.
4th, illustrate：
Fig. 1 is front view of the invention；
Fig. 2 is the general frame of the invention.
In figure：1 closed camera bellows, 2 closed scanning rooms, 3 sample cells, 4 dye liquor boxes, 5 dye liquor input and output pumps, 6 samples
Ultraviolet source is fixed in bottom of pond plate, 7 gel pieces, 8 sample cell cover plates, 9 motion scan ultraviolet sources, 10 motion scan CCD, 11.
5th, embodiment：
The present invention is described further below in conjunction with the accompanying drawings：
With reference to shown in Fig. 1, Fig. 2, this gel-colored scanned imagery device includes gel-colored unit, UV scanning imaging list
Member, UV scanning imaging unit and gel-colored unit must completely closed independences, it is ensured that UV scanning imaging unit is not dirty
Dye, corrosion.UV scanning imaging unit is arranged in closed scanning room 2, can complete the dyeing of gel, flushing, imaging and solidifying
Glue reclaim process, it is ensured that gel imaging analysis process is not polluted to environment.Gel-colored unit is arranged at closed dark
In case 1, closed camera bellows 1, which is placed in closed scanning room 2, forms one；Gel-colored unit include sample cell 3, dye liquor box 4,
Flushing liquor box, dye liquor input and output pump 5, flushing liquor input and output pump, sample cell bottom plate 6 are uviol plate, are that can make necessarily
The glass plate of the UV light permeability of wavelength, dye liquor box 4 connects sample cell 3 by dye liquor input and output pump 5, and flushing liquor box passes through punching
Washing lotion input and output pump, which is connected, places gel piece 7 on sample cell 3, sample cell bottom plate 6.UV scanning imaging unit includes movement and swept
Ultraviolet source 9, motion scan CCD10, fixed ultraviolet source 11 are retouched, the scanning of motion scan ultraviolet source 9 excites gel piece 7 to produce
Go out fluorescence signal, motion scan CCD10 scannings catch gel fluorescence signal；Motion scan CCD10 is passed through when the present invention is used
A/D converter connects computer.
An embodiment of the invention is：Closed camera bellows 1 is split with closed scanning room 2, by being fixed on
One is formed together, the bottom of camera bellows has bottom plate hole, and sample cell bottom plate 6 is fixed in bottom plate hole, have at the top of scanning room and sweep
Hole is retouched, bottom plate hole is corresponding with scanning holes to be set；Dye liquor input and output pump 5, flushing liquor input and output pump pass through corresponding glue
Pipe connects the bottom of sample cell 3, and the pool wall bottom of sample cell 3 is close to bottom of pond and is provided with dye liquor access interface and flushing liquor and comes in and goes out connect
Mouthful, the top of sample cell 3 is provided with sample cell cover plate 8, and sample cell 3 can be molded by plastic injected die and be manufactured.Dye liquor input is defeated
Going out pump 5 is used to gel-colored liquid being transported to sample cell 3 and is recovered to dye liquor liquid storage box, and flushing liquor input and output pump is used for will be solidifying
Glue flushing liquor is transported to sample cell 3 and is recovered to flushing liquor liquid storage box, and the two pumps are used to be that gel-colored liquid or flushing liquor are defeated
It is sent to sample cell 3 and is recovered to liquid storage box and pressure is provided, the conveying and recovery of liquid is realized by changing motor direction of rotation.It is solidifying
Glue is dyed and gel reclaims two processes and completed in closed camera bellows 1.
In the present invention closed camera bellows 1 and closed scanning room 2 can also be directly designed to it is integral, by it is closed not
Lateral partitions are set to be formed in the casing of printing opacity, closed camera bellows 1 under, is set in upper, closed scanning room 2 in lateral partitions
Bottom plate hole, sample cell bottom plate 6 is fixed in bottom plate hole.
Dye liquor liquid storage box is storage ethidium bromide（EB）Or the plastic casing of other gel-colored liquid；Flushing liquor liquid storage box is storage
Deposit the plastic casing of gel flushing liquor.
Motion scan ultraviolet source 9 is that strip is portable, and its wavelength is received according to 365 nanometers of sample demand apolegamy, 305
Rice, 254 nanometers, any one in 420 nanometers.
Motion scan CCD10 is the color CCD device of linear array three, and 20~30mm length is arranged in by thousands of photosensitive units
Constituted on CCD substrates.
Fixed ultraviolet source 11 is the ultraviolet transmissive light source needed for gel is reclaimed, and its wavelength is matched according to sample demand
Any one in 365 nanometers, 305 nanometers, 254 nanometers, 20 nanometers.
The dyeing imaging method of this gel-colored scanned imagery device：
The first step, gel piece 7 is placed in sample cell 3, starts dye liquor input and output pump 5 and dye liquor is input to sample cell by dye liquor box
3, gel is dyed.
Second step, the motor direction of rotation of change dye liquor input and output pump 5, dye liquor box is recovered to by dye liquor
3rd step, startup flushing liquor input and output pump impulse washing lotion are input to sample cell 3 by flushing liquor box, and gel is rinsed.
4th step, change flushing liquor input and output pump motor direction of rotation, flushing liquor box is recovered to by flushing liquor.
5th step, startup motion scan ultraviolet source 9, motion scan CCD10, obtain gel images.
6th step, opening sample cell cover plate 8, put on goggles, open fixed ultraviolet source 11, carry out gel recovery.
Claims (8)
1. a kind of gel-colored scanned imagery device, it is characterised in that：This gel-colored scanned imagery device contaminates including gel
Color element, UV scanning imaging unit, UV scanning imaging unit are arranged in closed scanning room (2), gel-colored unit
It is arranged in closed camera bellows (1), closed camera bellows (1), which is arranged in closed scanning room (2), forms one, gel-colored,
Rinse, gel is reclaimed and completed in closed camera bellows (1)；Gel-colored unit includes sample cell (3), dye liquor box (4), flushing liquor
Box, dye liquor input and output pump (5), flushing liquor input and output pump, sample cell bottom plate (6) are uviol plate, and dye liquor box (4) passes through
Dye liquor input and output pump (5) connection sample cell (3), flushing liquor box connects sample cell (3), sample by flushing liquor input and output pump
Gel piece (7) is placed on bottom of pond plate (6)；UV scanning imaging unit includes motion scan ultraviolet source (9), motion scan CCD
(10), fixed ultraviolet source (11), motion scan ultraviolet source (9) scanning excites gel piece (7) to produce fluorescence signal, mobile
Scan CCD (10) scannings and catch gel fluorescence signal.
2. gel-colored scanned imagery device according to claim 1, it is characterised in that：Described closed camera bellows (1)
It is split with closed scanning room (2), one is formed by being fixed together, closed camera bellows (1) bottom has bottom plate hole,
Sample cell bottom plate (6) is fixed in bottom plate hole, has scanning holes at the top of scanning room, and bottom plate hole is corresponding with scanning holes to be set；Dye
Liquid input and output pump (5), flushing liquor input and output pump connect the bottom of sample cell (3), sample cell (3) by corresponding sebific duct
Pool wall bottom is close to bottom of pond and is provided with dye liquor access interface and flushing liquor access interface, and sample cell is provided with sample at the top of (3)
Pond cover plate (8).
3. gel-colored scanned imagery device according to claim 1, it is characterised in that：Described closed camera bellows 1 ()
It is integral with closed scanning room (2), by setting lateral partitions to be formed in closed lighttight casing, in lateral partitions
Bottom plate hole is set, and sample cell bottom plate (6) is fixed in bottom plate hole；Dye liquor input and output pump (5), flushing liquor input and output pump lead to
Cross the bottom of corresponding sebific duct connection sample cell (3), sample cell (3) pool wall bottom be close to bottom of pond be provided with dye liquor access interface and
Sample cell cover plate (8) is provided with the top of flushing liquor access interface, sample cell (3).
4. the gel-colored scanned imagery device according to Claims 2 or 3, it is characterised in that：Described dye liquor liquid storage box
For storage ethidium bromide or the plastic casing of other gel-colored liquid；Flushing liquor liquid storage box is the plastic casing of storage gel flushing liquor.
5. gel-colored scanned imagery device according to claim 4, it is characterised in that：Described motion scan ultraviolet light
Source (9) is that strip is portable, and its wavelength is matched in 365 nanometers, 305 nanometers, 254 nanometers, 420 nanometers according to sample demand
Any one.
6. gel-colored scanned imagery device according to claim 5, it is characterised in that：Described motion scan CCD
(10) it is the color CCD device of linear array three, is constituted on the CCD substrates for being arranged in 20~30mm length by thousands of photosensitive units.
7. gel-colored scanned imagery device according to claim 6, it is characterised in that：Described fixation ultraviolet source
(11) for gel reclaim needed for ultraviolet transmissive light source, its wavelength according to sample demand apolegamy 365 nanometers, 305 nanometers,
Any one in 254 nanometers, 20 nanometers.
8. a kind of dyeing imaging method of the gel-colored scanned imagery device described in Claims 2 or 3, it is characterised in that：It is first
First, completed in closed camera bellows (1) gel-colored and rinse two processes：
A, gel piece (7) is placed on sample cell bottom plate (6), starts dye liquor input and output pump (5) dye liquor is defeated by dye liquor box (4)
Enter to sample cell (3), gel is dyed；
B, change dye liquor input and output pump (5) motor direction of rotation, dye liquor box (4) is recovered to by dye liquor；
C, startup flushing liquor input and output pump, flushing liquor are input to sample cell (3) by flushing liquor box, gel are rinsed；
D, change flushing liquor input and output pump motor direction of rotation, flushing liquor box is recovered to by flushing liquor；
Then, start motion scan ultraviolet source (9), motion scan CCD (10), obtain gel images；
Finally, sample cell cover plate (8) is opened, goggles is put on, fixed ultraviolet source (11) is opened, carries out gel recovery.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710605700.7A CN107271419B (en) | 2017-07-24 | 2017-07-24 | Gel dyeing scanning imaging device and dyeing imaging method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710605700.7A CN107271419B (en) | 2017-07-24 | 2017-07-24 | Gel dyeing scanning imaging device and dyeing imaging method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107271419A true CN107271419A (en) | 2017-10-20 |
| CN107271419B CN107271419B (en) | 2020-06-19 |
Family
ID=60077987
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710605700.7A Active CN107271419B (en) | 2017-07-24 | 2017-07-24 | Gel dyeing scanning imaging device and dyeing imaging method thereof |
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| Country | Link |
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| CN (1) | CN107271419B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022211431A1 (en) * | 2021-03-31 | 2022-10-06 | 주식회사 씨티셀즈 | Integrated cell selection apparatus |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5147522A (en) * | 1987-03-16 | 1992-09-15 | Helena Laboratories Corporation | Automatic electrophoresis apparatus and method |
| US5230781A (en) * | 1984-03-29 | 1993-07-27 | Li-Cor, Inc. | Sequencing near infrared and infrared fluorescence labeled DNA for detecting using laser diodes |
| CN103575584A (en) * | 2013-08-12 | 2014-02-12 | 北京林业大学 | Gel electrophoresis dyeing device |
| CN105026923A (en) * | 2013-04-30 | 2015-11-04 | 电子系统股份有限公司 | Electrophoresis method and electrophoresis device |
| CN106053188A (en) * | 2016-06-24 | 2016-10-26 | 广东医学院 | Gel dyeing-decoloration integrated equipment and control method thereof |
-
2017
- 2017-07-24 CN CN201710605700.7A patent/CN107271419B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5230781A (en) * | 1984-03-29 | 1993-07-27 | Li-Cor, Inc. | Sequencing near infrared and infrared fluorescence labeled DNA for detecting using laser diodes |
| US5147522A (en) * | 1987-03-16 | 1992-09-15 | Helena Laboratories Corporation | Automatic electrophoresis apparatus and method |
| CN105026923A (en) * | 2013-04-30 | 2015-11-04 | 电子系统股份有限公司 | Electrophoresis method and electrophoresis device |
| CN103575584A (en) * | 2013-08-12 | 2014-02-12 | 北京林业大学 | Gel electrophoresis dyeing device |
| CN106053188A (en) * | 2016-06-24 | 2016-10-26 | 广东医学院 | Gel dyeing-decoloration integrated equipment and control method thereof |
Non-Patent Citations (5)
| Title |
|---|
| 中国农业科学院哈尔滨兽医研究所: "《兽医微生物学》", 31 December 2013 * |
| 刘静: "《分子生物学实验指导》", 31 October 2015 * |
| 方向明等: "《麻醉实验学》", 31 March 2013 * |
| 杜军等: "《现代药学生物技术综合实验教程》", 31 December 2014 * |
| 郭定宗: "《兽医实验室诊断指南》", 30 June 2013 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022211431A1 (en) * | 2021-03-31 | 2022-10-06 | 주식회사 씨티셀즈 | Integrated cell selection apparatus |
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| CN107271419B (en) | 2020-06-19 |
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