CN107271393A - A kind of detection method of Fast Evaluation ferment fat-reducing effect - Google Patents
A kind of detection method of Fast Evaluation ferment fat-reducing effect Download PDFInfo
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- CN107271393A CN107271393A CN201710563972.5A CN201710563972A CN107271393A CN 107271393 A CN107271393 A CN 107271393A CN 201710563972 A CN201710563972 A CN 201710563972A CN 107271393 A CN107271393 A CN 107271393A
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- 230000000694 effects Effects 0.000 title claims abstract description 82
- 238000011156 evaluation Methods 0.000 title claims abstract description 32
- 238000001514 detection method Methods 0.000 title claims abstract description 23
- 108090000790 Enzymes Proteins 0.000 claims abstract description 68
- 102000004190 Enzymes Human genes 0.000 claims abstract description 68
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 42
- 239000008103 glucose Substances 0.000 claims abstract description 41
- 238000000354 decomposition reaction Methods 0.000 claims abstract description 14
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims abstract description 13
- 101100256850 Drosophila melanogaster EndoA gene Proteins 0.000 claims abstract description 6
- 230000015556 catabolic process Effects 0.000 claims abstract description 3
- 239000003153 chemical reaction reagent Substances 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 10
- 238000009413 insulation Methods 0.000 claims description 5
- 230000031700 light absorption Effects 0.000 claims description 5
- 230000002779 inactivation Effects 0.000 claims description 4
- 238000009835 boiling Methods 0.000 claims description 3
- 238000000870 ultraviolet spectroscopy Methods 0.000 claims description 3
- 238000010521 absorption reaction Methods 0.000 claims description 2
- 230000003287 optical effect Effects 0.000 claims 1
- 238000004611 spectroscopical analysis Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 10
- 235000000346 sugar Nutrition 0.000 abstract description 6
- 238000005516 engineering process Methods 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 abstract description 2
- 238000007689 inspection Methods 0.000 abstract description 2
- 230000001590 oxidative effect Effects 0.000 abstract description 2
- 230000008569 process Effects 0.000 abstract description 2
- 230000035945 sensitivity Effects 0.000 abstract description 2
- 229940088598 enzyme Drugs 0.000 description 44
- 239000000243 solution Substances 0.000 description 11
- RGHNJXZEOKUKBD-SQOUGZDYSA-N D-gluconic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 5
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 5
- 108010015776 Glucose oxidase Proteins 0.000 description 3
- 239000004366 Glucose oxidase Substances 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 229950006191 gluconic acid Drugs 0.000 description 3
- 229940116332 glucose oxidase Drugs 0.000 description 3
- 235000019420 glucose oxidase Nutrition 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 2
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 2
- 230000008033 biological extinction Effects 0.000 description 2
- 239000012482 calibration solution Substances 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 235000012208 gluconic acid Nutrition 0.000 description 2
- 239000000174 gluconic acid Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- JRBJSXQPQWSCCF-UHFFFAOYSA-N 3,3'-Dimethoxybenzidine Chemical compound C1=C(N)C(OC)=CC(C=2C=C(OC)C(N)=CC=2)=C1 JRBJSXQPQWSCCF-UHFFFAOYSA-N 0.000 description 1
- 206010006326 Breath odour Diseases 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 208000032139 Halitosis Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 241000700647 Variola virus Species 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 238000011481 absorbance measurement Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 150000002596 lactones Chemical class 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000008447 perception Effects 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 229940095548 phenazo Drugs 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 150000004053 quinones Chemical class 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
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- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention belongs to detect inspection technology field, a kind of detection method of Fast Evaluation ferment fat-reducing effect is disclosed.Detection method obtains enzyme activity by the amount of analytical unit time endo enzyme decomposition glucose so as to calculate, and is evaluated.Enzyme activity is 1mL or 1g ferment breakdown of glucose amount hourly.Evaluation criterion is (μm ol/h)：Enzyme activity >=60,4 grade；40≤enzyme activity<60,3 grades；20≤enzyme activity<40,2 grades；0≤enzyme activity<20,1 grades；Enzyme activity<0,0 grade.The present invention carries out lateral comparison by carrying out glucolase vigor test to ferment, reflects its fat-reducing effect.First using the decrement of glucose in the enzyme phenol oxidizing process analytical unit time in method, detection method is easy to operate, and speed of experiment is fast, and sensitivity is high.Reflect the ability of the decomposition sugar of the ferment of unit quantity with glucose decrement, calculate ferment enzyme activity to evaluate its fat-reducing effect with this.
Description
Technical field
The invention belongs to detect inspection technology field, more particularly to a kind of detection side of Fast Evaluation ferment fat-reducing effect
Method.
Background technology
Ferment (enzyme) is also known as enzyme, be can in organism produced by the active macromolecules with catalytic action.
Recently as the development of society, people are more and more stronger to self health perception and health care consciousness, and market is upper recently
Go a kind of commodity --- pectase, it, which is turned on, can eliminate the effects such as halitosis, abdomen, small pox and constipation, but at present still
There is no any detection method to can determine that its effect and security.
Ferment extensively liked as a kind of emerging product by consumer, but the good and bad jumbled together for ferment product on the market, portion
The exaggeration of point advertising effect, wherein just including ferment has this saying of weight losing function, and the detection in this industry to ferment
Standard is still in a blank, and consumer is also to its concept obfuscation.
Fat main cause is because hoarding for fat is excessive, so as to easily trigger the diseases such as hypertension.Human body lactones
The main source of fat comes from the sugared decomposition of intake.So under normal operation, it is more that carbohydrate is taken in, what body weight rose gets over
It hurry up.
The content of the invention
In order to overcome the shortcomings and deficiencies of the prior art described above, primary and foremost purpose of the invention is to provide a kind of Fast Evaluation
The detection method of ferment fat-reducing effect.
The present invention is detected by determining capacity of decomposition of the ferment to glucose within the unit interval, and carries out quantitative pair
Than for evaluating its fat-reducing effect.
The purpose of the present invention is realized by following proposal：
A kind of detection method of Fast Evaluation ferment fat-reducing effect, passes through analytical unit time endo enzyme decomposition glucose
Amount obtains enzyme activity so as to calculate, and is evaluated.
Described enzyme activity is 1mL the or 1g ferment breakdown of glucose amount of 1 hour.
Evaluation criterion is：
Enzyme activity >=60 μm ol/h, 4 grades；
40 μm of ol/h≤enzyme activities<60 μm of ol/h, 3 grades；
20 μm of ol/h≤enzyme activities<40 μm of ol/h, 2 grades；
0 μm of ol/h≤enzyme activity<20 μm of ol/h, 1 grade；
Enzyme activity<0 μm of ol/h, 0 grade.
Represent that the ferment has notable fat-reducing effect when evaluation result is 4 grades；
Represent that the ferment has stronger fat-reducing effect when evaluation result is 3 grades；
Represent that the ferment has fat-reducing effect when evaluation result is 2 grades, but effect is general；
Represent that the ferment has weaker fat-reducing effect when evaluation result is 1 grade；
Represent that the ferment hardly possesses weight losing function when evaluation result is 0 grade, or even itself contains sugar.
Above-mentioned detection method is specially：By the amount of ultraviolet spectrometry analytical unit time endo enzyme decomposition glucose so as to
Calculating obtains enzyme activity, and is evaluated.
Described referred to by ultraviolet spectrometry determines light absorption value after decomposition, reference standard curve, concentration after being decomposed, according to
The amount that concentration conversion is decomposed before decomposing, obtains enzyme activity, and evaluated so as to calculate.
More specifically it may include following steps：By the way that ferment is added in glucose solution, 37 DEG C of water-baths, boiling water bath goes out
It is living, enzyme phenol reagent is added, 37 DEG C of water-bath insulation reactions determine its light absorption value, calculating obtains enzyme activity, and is evaluated.
The time (i.e. ferment adds the glucose sugar juice reaction time) of 37 DEG C of water-baths can adjust as needed, such as
Can be 30min, enzyme activity is converted to decomposition amount hourly during final evaluation.
The time of the inactivation is Conventional Time, such as 1min.
The time for adding insulation reaction after enzyme phenol reagent is preferably 10min.
The enzyme phenol reagent is the enzyme phenol reagent of conventional determining concentration of glucose.
The measure UV absorption is determined preferably at 500nm.
The inventive method obtains enzyme activity by the amount of analytical unit time endo enzyme decomposition glucose so as to calculate, and goes forward side by side
Row is evaluated.
Detection method can be carried out anti-using Glucose estimation kit (glucose oxidase method/single reagent method)
Should, the ability of ferment decomposition glucose is measured indirectly.In the available reagent box of this area solution is calibrated containing Glucose standards
(5.55mmol/L), glucose standards solution (10.00mmol/L), Glucose standards storing solution (100.00mmol/L), enzyme phenol
Mix reagent.Operated according to kit specification requirement, wherein, the time of insulation reaction is after addition enzyme phenol reagent
10min。
The principle of the invention：Glucose oxidase (GOD) catalysis glucose is oxidized to gluconic acid (in maltonic acid δ
Ester), and produce hydrogen peroxide：
Glucose+2H2O+O2→ gluconic acid+2H2O2
H2O2+ 4- amino antipyrine → red quinones+H2O
In the presence of chromogenic oxygen acceptor (such as dianisidine, 4-AA phenazo), peroxidase
Catalyzing hydrogen peroxide, aoxidizes chromogen substance, generates colored compound.Its shade contains with serum glucose within the specific limits
Amount is directly proportional, and absorbance is determined at 500nm, concentration of glucose can be measured by being compared with standard pipe.
The present invention can convert according to original liquid concentration and be decomposed by determining concentration of glucose after the quantitative ferment reaction of addition
The amount of glucose reduction, enzyme activity is obtained so as to calculate, and ferment fat-reducing effect is evaluated with this.
The present invention carries out lateral comparison by carrying out glucolase vigor test to multiple ferment samples, reflects that it subtracts
Fertilizer efficiency fruit.First using the decrement of glucose in the enzyme phenol oxidizing process analytical unit time in method, detection method is easy to operate,
Speed of experiment is fast, and sensitivity is high.Reflect the ability of the decomposition sugar of the ferment of unit quantity with glucose decrement, calculated with this
Ferment enzyme activity is to evaluate its fat-reducing effect.
Brief description of the drawings
Fig. 1 is enzyme activity determination standard curve, wherein, y=0.0486x+0.0148, R2=0.9793.
Embodiment
With reference to embodiment, the present invention is described in further detail, but the implementation of the present invention is not limited to this.
The material being related in the following example can be obtained from commercial channel.
The measure of standard curve：
Precision weighs dextrose standard sample 1.8016g, is dissolved in water, and is transferred in 1L volumetric flasks, is settled to graduation mark, i.e.,
Into 10mmol/L Glucose standards storing solutions, every milliliter of μ g containing glucose 1801.6.
Take Glucose standards storing solution 0mL, 5mL, 10mL, 15mL, 20mL in 100mL volumetric flasks respectively, add water to quarter
It (is that 0.0mmol/L, 5.0mmol/L, 10.0mmol/L, 15.0mmol/L, 20.0mmol/L glucose are molten equivalent to concentration to spend line
Liquid).20 μ L are respectively taken in test tube, 3.0mL enzyme phenol mix reagent (Zhejiang Eastern Europe) is added, 37 DEG C of water-baths are incubated 10min.
Its light absorption value is determined at 500nm, record draws standard curve, sees Fig. 1.
The measure of sample：Using Glucose estimation kit (glucose oxidase method/single reagent method), wherein containing grape
Standard for Sugars calibration solution (5.55mmol/L), glucose standards solution (10.00mmol/L), Glucose standards storing solution
(100.00mmol/L), distilled water, enzyme phenol mix reagent.
The sample commercially available to 7 is measured：4mL glucose standards solutions are taken, 1.0mL ferment, 37 DEG C of water is separately added into
Bath reaction 30min, boiling water bath inactivation 1min, enzyme-added phenol reagent, 37 DEG C of water-baths are incubated 10min, its extinction are determined at 500nm
Value, adds 1.0mL ferment using 4mL distilled water and is managed as calibration, calculating obtains enzyme activity, and is evaluated.
1st, glucose reduces concentration (mmol/L)
In formula：c0For glucose standards solution concentration；csSolution concentration is calibrated for Glucose standards；A1To determine pipe extinction
Degree；AsFor calibration pipe absorbance.
2、
In formula：c1By reduction concentration of glucose；V is sample pretreatment volume；Δ t be sample processing time (i.e. plus
Enter the water-bath time after ferment).
3rd, result of calculation, is shown in Table 1~3.
The concentration of standard solution of table 1 determines table
The calibration solution absorbance measurement table of table 2
| Sample number into spectrum | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
| Absorbance A | 0.024 | 0.024 | 0.122 | 0.348 | 0.013 | 0.016 | 0.033 |
The sample enzyme activity determination table of table 3
It can be seen from enzyme activity compares evaluation criterion：
The enzyme activity of sample 1 (μm ol/h)=72.813,4 grade.
The enzyme activity of sample 2 (μm ol/h)=39.473,2 grade.
The enzyme activity of sample 3 (μm mol/h)=5.846,1 grade.
The enzyme activity of sample 4 (μm ol/h)=17.361,1 grade.
The enzyme activity of sample 5 (μm ol/h)=5.386,1 grade.
The enzyme activity of sample 6 (μm ol/h)=28.480,2 grade.
The enzyme activity of sample 7 (μm ol/h)=- 0.367,0 grade.
Evaluation criterion：
Enzyme activity >=60 μm ol/h, 4 grades；
40 μm of ol/h≤enzyme activities<60 μm of ol/h, 3 grades；
20 μm of ol/h≤enzyme activities<40 μm of ol/h, 2 grades；
0 μm of ol/h≤enzyme activity<20 μm of ol/h, 1 grade；
Enzyme activity<0 μm of ol/h, 0 grade.
Represent that the ferment has notable fat-reducing effect when evaluation result is 4 grades；
Represent that the ferment has stronger fat-reducing effect when evaluation result is 3 grades；
Represent that the ferment has fat-reducing effect when evaluation result is 2 grades, but effect is general；
Represent that the ferment has weaker fat-reducing effect when evaluation result is 1 grade；
Represent that the ferment hardly possesses weight losing function when evaluation result is 0 grade, or even itself contains sugar.
Above-described embodiment is preferably embodiment, but embodiments of the present invention are not by above-described embodiment of the invention
Limitation, other any Spirit Essences without departing from the present invention and the change made under principle, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (10)
1. a kind of detection method of Fast Evaluation ferment fat-reducing effect, it is characterised in that decomposed by analytical unit time endo enzyme
The amount of glucose obtains enzyme activity so as to calculate, and is evaluated.
2. the detection method of Fast Evaluation ferment fat-reducing effect according to claim 1, it is characterised in that：Described enzyme activity
Power is 1mL the or 1g ferment breakdown of glucose amount of 1 hour.
3. the detection method of Fast Evaluation ferment fat-reducing effect according to claim 1, it is characterised in that evaluation criterion is：
Enzyme activity >=60 μm ol/h, 4 grades；
40 μm of ol/h≤enzyme activities<60 μm of ol/h, 3 grades；
20 μm of ol/h≤enzyme activities<40 μm of ol/h, 2 grades；
0 μm of ol/h≤enzyme activity<20 μm of ol/h, 1 grade；
Enzyme activity<0 μm of ol/h, 0 grade.
4. the detection method of Fast Evaluation ferment fat-reducing effect according to claim 1, it is characterised in that be specially：Pass through
The amount of ultraviolet spectrometry analytical unit time endo enzyme decomposition glucose obtains enzyme activity so as to calculate, and is evaluated.
5. the detection method of Fast Evaluation ferment fat-reducing effect according to claim 4, it is characterised in that：It is described to pass through purple
Outer optical spectroscopy refers to light absorption value after measure is decomposed, and reference standard curve, concentration after being decomposed is obtained according to concentration conversion before decomposition
The amount of decomposition, obtains enzyme activity, and evaluated so as to calculate.
6. the detection method of Fast Evaluation ferment fat-reducing effect according to claim 1, it is characterised in that specifically include with
Lower step：By the way that ferment is added in glucose solution, 37 DEG C of water-baths, boiling water bath inactivation adds enzyme phenol reagent, 37 DEG C of water
Insulation reaction is bathed, its light absorption value is determined, calculating obtains enzyme activity, and is evaluated.
7. the detection method of Fast Evaluation ferment fat-reducing effect according to claim 6, it is characterised in that：37 DEG C of water
The time of bath reaction is 30min.
8. the detection method of Fast Evaluation ferment fat-reducing effect according to claim 6, it is characterised in that：The inactivation
Time is 1min.
9. the detection method of Fast Evaluation ferment fat-reducing effect according to claim 6, it is characterised in that：The addition enzyme
The time of insulation reaction is 10min after phenol reagent.
10. the detection method of Fast Evaluation ferment fat-reducing effect according to claim 6, it is characterised in that：It is described to determine
UV absorption is determined at 500nm.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101676719A (en) * | 2008-09-19 | 2010-03-24 | 河南瑞驰生物科技有限公司 | Method for measuring diastatic enzyme activity using a spectrophotometer |
| CN105586388A (en) * | 2016-03-14 | 2016-05-18 | 河南大学 | Measuring method for activity of glucose oxidase |
| CN106841069A (en) * | 2017-02-24 | 2017-06-13 | 北京挑战生物技术有限公司 | A kind of glucose oxidase activity quick measuring reagent box and its detection method |
-
2017
- 2017-07-12 CN CN201710563972.5A patent/CN107271393A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101676719A (en) * | 2008-09-19 | 2010-03-24 | 河南瑞驰生物科技有限公司 | Method for measuring diastatic enzyme activity using a spectrophotometer |
| CN105586388A (en) * | 2016-03-14 | 2016-05-18 | 河南大学 | Measuring method for activity of glucose oxidase |
| CN106841069A (en) * | 2017-02-24 | 2017-06-13 | 北京挑战生物技术有限公司 | A kind of glucose oxidase activity quick measuring reagent box and its detection method |
Non-Patent Citations (4)
| Title |
|---|
| BARBARA JANSON COHEN: "《曼姆勒人体健康与疾病》", 31 July 2014, 金盾出版社 * |
| 刘福堃 等: "《微生物酵素及其应用》", 31 January 2014, 辽宁科学技术出版社 * |
| 李丕武 等: "两种葡萄糖氧化酶活力测定方法的比较", 《食品工业科技》 * |
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Application publication date: 20171020 |