CN107271391A - Room temperature solid ionic liquid is used as the forulic acid in extractant separation determination Radix Angelicae Sinensis - Google Patents
Room temperature solid ionic liquid is used as the forulic acid in extractant separation determination Radix Angelicae Sinensis Download PDFInfo
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Abstract
Title of the present invention is " room temperature solid ionic liquid is as the forulic acid in extractant separation determination Radix Angelicae Sinensis ", affiliated technical field is to determine its content after ionic liquid at room temperature separates forulic acid as the liquid-solid extraction of extractant, and solving the technical scheme of the problem mainly has：By room temperature be solid in liquid-solid extraction device of the present invention, the ionic liquid hexafluorophosphoric acid N butyl-pyridiniums that more than 65 DEG C are liquid（[BPy]PF6）As extractant at 80 DEG C, the forulic acid in Radix Angelicae Sinensis is extracted into [BPy] PF under certain condition6In；It is cooled to after room temperature, by [BPy] PF containing forulic acid6Solid phase is put into liquid-solid extraction device, is added the NaOH solution of debita spissitudo and is heated to 80 DEG C, forulic acid its content of spectrophotometry after solid-liquid back extraction in aqueous phase.
Description
Technical field
The technical field determined after being separated the present invention relates to ionic liquid at room temperature as the leaching of extractant, specifically
Be related to by solid of room temperature, more than the 65 DEG C ionic liquids for liquid -- hexafluorophosphoric acid-N- butyl-pyridiniums（[BPy]PF6）As
After forulic acid in extractant, leaching separation Radix Angelicae Sinensis, the content of spectrophotometry ferulic acid from Chinese angelica.
Background technology
When being classified as samphire Angelica sinensis（Oliv.）Diels root, is TCM Gynecology medicine.Forulic acid
It is the active component in Radix Angelicae Sinensis, forulic acid has sedation, can increase coronary blood flow, protection ischemic myocardium；Due to α acceptors
There is blocking effect, forulic acid can suppress aortal smooth muscle contraction, resist the liter of first chloramines, phyenlephrinium beta adrenergic etc.
Pressure is acted on；Sodium ferulate is conducive to improving imbalance of supply and demand of the cardiac muscle to oxygen, also with antiplatelet aggregative activity.Forulic acid can be light
Micro- activated lymphocyte, promotes the propagation of gland lymphocyte；Forulic acid has certain Robust speaker feature effect；A variety of methods confirmations,
Forulic acid is that the hydroxyl on a kind of antioxidant, its phenyl ring is oxidation resistant active group, can also eliminate free radical, suppresses oxygen
Change reaction and radical reaction, and combined with biological film phospholipid, the infringement of the antagonism free radical to tissue such as protection membrane lipid, production
Raw antiatherosclerosis effect.Human body can absorb and be conducive to discharging from urine, be a kind of foolproof medicine（River
Su Xin medical colleges compile, dictionary of medicinal plant, the first volume, in July, 1977,876-879；Ou Shiyi etc., Chinese medicine, 2001,24,220-
221）.
Due to containing plurality of active ingredients in Radix Angelicae Sinensis, determined by ultraviolet spectrophotometry ferulic acid from Chinese angelica content is individually used
When, serious interference, deviation is big.Therefore, with determined by ultraviolet spectrophotometry after the measure of forulic acid or separation（Su Xiulan etc.,
Agriculture of Anhui science, 2016,44,142-144）, or use high performance liquid chromatography（Chen Han equalitys, Chinese herbal medicine, 1988,
19, 15-16）, thin layer chromatography scanning（Ren Yanjun etc., Chinese Pharmaceutical Journal, 1991,26,136-138）, HPCE
（Shao Yuanfu etc., second medical academy's journal, 1999,20,702-703）Measure is directly separated etc. method.However, these methods exist
Using inflammable, explosive volatile organic solvents such as petroleum ether, methanol in separation process, injury is caused to environment.Therefore, it is green
Color environment-friendly type solvent replaces conventional organic solvents tool to be of great significance.
Ionic liquid（ILs）It is that fusing point is less than 100 DEG C of ionic compound, due to vapour pressure that it almost can not determined
It is considered as Green Chemistry reagent.ILs is the novel dissolvent of a class physical property and the formation of SOLUTION PROPERTIES specific combination, is scope
The good polar solvent of extensive compound, this makes them turn into the focus of research and application in separation science（T.
Welton, Chemical Review, 1999, 99, 2071–2083）.Many documents（N. Gjineci, et al.,
Fluid Phase Equilibria, 2016, 424, 1-7；D. Wu et al., Journal of
Chromatography A, 2015, 1395, 65-72; Y. Xie et al., Applied Energy, 2014,
136,325-335; B. Cabo et al. Fluid Phase Equilibria, 2013, 356, 126-135; Z.
Pan et al., Applied Mechanics and Materials, 2012,189, 75-79; Y. Deng et al.,
Journal of Hazardous Materials, 2011,198, 165-174）Report ionic liquid be used for separate grind
Study carefully, however, these reports ILs overwhelming majority used is imidazoles, and to room temperature be solid, the pyridines that more than 65 DEG C are liquid
ILs is used for the report of the research only extracting metals ion separated（Z Pan et al., Materials Research
Innovations, 2015, 19, s283-287; Z Pan et al., Molecules, 2015, 20, 22137–
22145）.
Leaching be using a kind of molecular weight it is larger, at room temperature be solid organic compound, such as naphthalene（Fusing point 81
℃）, biphenyl（70.5 DEG C of fusing point）, benzophenone（73 DEG C of fusing point）And paraffin（As the solid paraffin obtained by oil, cut is different,
Fusing point is at 40 DEG C -90 DEG C）Deng, can be effective, fast under the collective effect of certain extractant when heating is melted to liquid
Speed, quantitatively extract water-insoluble, heat-staple metal complex.In the solid solvent used in leaching method, hexichol first
Ketone（Y. Shigetomi et al., Talanta, 1980, 27, 1079-1080）, biphenyl（T. Kojima et al.,
Analyst, 1982, 107, 519-524）It is less Deng the application due to expensive, using it is more be naphthalene（Wu Ruilin etc.,
Assay office, 1988,7,34-39）And paraffin（Gao Jinzhang etc., analytical chemistry, 1992,20,297-299）.2013, I
Report with room temperature for solid ionic liquid --- hexafluorophosphoric acid-N- butyl-pyridiniums are as solid solvent, benzoyl third
Ketone（Z. Pan, et al., Advanced Materials Research, 2013, 734-737, 906-910）, 8- hydroxyls
Base quinoline（Z. Pan et al., Applied Mechanics and Materials, 2013, 401-403, 817-
821）As Extraction behavior of the extractant at 80 DEG C to rare earth element (RE), as a result show, [BPy] PF6- HQ-RE systems
Liquid-liquid extraction (LLE) behavior is similar with liquid-liquid extraction, and [BPy] PF6The SLE Extraction behaviors of-HA-RE systems are different from LLE.
The present invention is with ionic liquid --- [BPy] PF of environmental type6Substitute petroleum ether, methanol etc. poisonous, volatile
Organic solvent as solid phase extraction agent, after the forulic acid in leaching separation Radix Angelicae Sinensis, in spectrophotometry Radix Angelicae Sinensis
The content of forulic acid.As a result show, with 0.8 g [BPy] PF6The forulic acid in a certain amount of Radix Angelicae Sinensis can be extracted completely；0.02
Ferulaic acid content is determined in 320 nm after mol/L NaOH back extraction, is as a result satisfied with.
The content of the invention
In view of the shortcomings of the prior art, it is an object of the invention to provide room temperature solid ionic liquid [BPy] PF6It is used as extraction
Take after the forulic acid in agent, leaching separation Radix Angelicae Sinensis, the method for the content of spectrophotometry ferulic acid from Chinese angelica.Should
Method is beneficial to realize resources conservation, the purpose of environmental protection.
In order to solve the above technical problems, the technical solution used in the present invention is：1、[BPy]PF6The synthesis of ionic liquid
And characterize；2nd, leaching and the device of back extraction；3rd, the leaching of ferulic acid from Chinese angelica and back extraction；4th, in Radix Angelicae Sinensis Ah
The measure of Wei's acid.
[BPy] PF6The synthesis of ionic liquid and sign include procedure below：
[BPy]PF6Synthesis：The bromination of n-butane of a certain amount of pyridine sum is accurately weighed in three-neck flask, a little ring is added
Mechanical agitation 10h under hexane, 65 DEG C of heating water baths, obtains two layers of liquid；Lower floor is yellow liquid, and upper strata is milky white liquid,
Incline upper strata, by lower floor's liquid in Rotary Evaporators 90 DEG C of evaporation 2h, obtain faint yellow solid, washed with ethyl acetate, put
24h is dried at 60 DEG C into vacuum drying chamber, faint yellow solid is obtained, is for bromination-N- butyl-pyridiniums（[BPy]Br）.It is accurate again
A certain amount of [BPy] Br and Potassium Hexafluorophosphate are really weighed in round-bottomed flask, a little acetone is added, in 30 DEG C of constant temperature water bath heating
Lower stirring 24h, suction filtration removes metafiltration liquid and obtains white solid powder after rotary evaporation, cooling at 50 DEG C, is washed with distillation
Wash, dry 24h at 50 DEG C in vacuum drying chamber after suction filtration after obtain white powdery solids, be for [BPy] PF6.Above institute
The chemical reaction for stating process is as shown in Figure 1.
[BPy]PF6Sign：[BPy]PF6Chemical constitution as shown in Fig. 2 its chemical constitution is by infrared spectrum and mass spectrum
Characterize, purity is more than 98%.Measure [BPy] PF6Proportion be 1.2501g/mL, fusing point be 73.8 DEG C -75.5 DEG C.
The leaching and the device of back extraction are as shown in Figure 3.In Fig. 3, extraction or back extraction reaction are in a folder
Carried out in layer glass extractor, reaction drives magneton stirring to be strengthened by magnetic stirring apparatus；Thermostatic water-circulator bath passes through hot water
Entrance injects the hot water of required temperature in the glass sandwich of laminated glass extractor, to extractor provide the hot water after heat by
The temperature that hot water outlet passes back into thermostatic water-circulator bath when being heated to hot water inlet enters laminated glass by hot water inlet again
Extractor, so circulation provide heat to extract or being stripped reaction；It is suitable for reading that glass stopper should fill in laminated glass extractor in good time
In to prevent in the aqueous solution of upper strata a small amount of hydrone evaporation.
The leaching of the ferulic acid from Chinese angelica and back extraction are in above-mentioned leaching device, with a certain amount of
[BPy]PF6Extract a certain amount of forulic acid after processing in Radix Angelicae Sinensis and [BPy] PF is entered with stripping solution back extraction6In Ah
Wei's acid.Detailed in Example.
The measure of the ferulic acid from Chinese angelica is to carry out AAS survey to the forulic acid after back extraction in above-mentioned Radix Angelicae Sinensis
It is fixed, to determine the content of ferulic acid from Chinese angelica.Detailed in Example.
Relative to prior art, the present invention, which at least contains, to be had the advantage that：First, ionic liquid is environmentally friendly as one kind
Reagent, its chemical stability and heat endurance are good, non-volatile, nonflammable, solvability is strong, electrochemical window is wide, property is adjustable
The features such as saving and can recycle, has high Development volue in many-side, has very big development space in future；The
Two, ionic liquid [BPy] PF6The leaching system constituted with Radix Angelicae Sinensis treatment fluid applies to the height of forulic acid extract and separate
Extraction yield system；3rd, high with extraction efficiency during the solid-liquid system extract and separate forulic acid, split-phase is fast, and in extraction process
Will not occur emulsion.
Brief description of the drawings
Fig. 1 is synthesis [BPy] PF of room temperature solid ionic liquid of the present invention as the forulic acid in extractant separation determination Radix Angelicae Sinensis6
Chemical reaction process schematic diagram.
Fig. 2 is [BPy] PF of room temperature solid ionic liquid of the present invention as the forulic acid in extractant separation determination Radix Angelicae Sinensis6Change
Learn structural representation.
Fig. 3 be room temperature solid ionic liquid of the present invention as the forulic acid in extractant separation determination Radix Angelicae Sinensis leaching with
The schematic device of solid-liquid back extraction.
Fig. 4 is room temperature ph value of aqueous phase of the room temperature solid ionic liquid of the present invention as the forulic acid in extractant separation determination Radix Angelicae Sinensis
Influence schematic diagram to extracting forulic acid.
Fig. 5 is for room temperature solid ionic liquid of the present invention as the NaOH of the forulic acid in extractant separation determination Radix Angelicae Sinensis to forulic acid
Back extraction efficiency schematic diagram.
Embodiment
With reference to embodiment, the present invention is described in further detail, but the implementation of the present invention is not limited to this.
Embodiment
Present embodiments provide at 80 DEG C with [BPy] PF6For extractant, leaching separates the forulic acid in Radix Angelicae Sinensis
Afterwards, the content of spectrophotometry ferulic acid from Chinese angelica.Radix Angelicae Sinensis used in the present embodiment is purchased from Quanzhou big pharmacy of new Southern Star.
The processing of Radix Angelicae Sinensis raw material, the Radix Angelicae Sinensis drying for accurately weighing 10.0000 g or so is put into mortar and smashed, and is put into
Dissolved by heating in beaker, heat filtering, it is stand-by to be transferred to constant volume in 100 mL volumetric flasks after filtrate cooling.
The leaching process of forulic acid：0.8 g[BPy]PF6With contain a certain amount of forulic acid or a certain amount of Radix Angelicae Sinensis processing
10 mL aqueous phases of liquid and cushioning liquid are put into leaching device, and solution is with magnetic stirring apparatus in (80 ± 0.1) DEG C
Taken out after 10 min of lower stirring and be cooled to room temperature, [BPy] PF6After solidifying two-phase is automatically separated, pH meter determines aqueous phase acidity, water
Ferulaic acid content spectrophotometer is determined at 321 nm in phase.Extract into [BPy] PF6In forulic acid with the 0.02 of 10 ml
Mol/L NaOH solutions are determined after being stripped 10 min at 80 DEG C.The extraction yield of forulic acid（%）Be extract in IL forulic acid amount with
The ratio of forulic acid amount in initial aqueous phase.
[BPy]PF6Leaching and back extraction to forulic acid：The experiment of the present embodiment shows, 0.8 g [BPy] PF6
Can aqueous phase extracted volume be completely 10 mL, 2.5 × 10-2 Mol/L forulic acid.Fig. 4 is pH value to [BPy] PF6Extract Ah
The influence of Wei's acid.As seen from the figure, extraction of the pH value to forulic acid has large effect, and extraction takes the lead in increasing with the increase of pH value
Greatly, pH>4 extraction yields decrease.The present embodiment selection pH=3.34 are used as optimal extraction acidity.Extract into [BPy] PF6
In forulic acid can be stripped with NaOH solution, as shown in Figure 5.As seen from Figure 5,0.02 mol/L NaOH can be by [BPy] PF6
In forulic acid be stripped completely.
It is Y=0.0732X+0.054 that the present embodiment, which obtains determining forulic acid working curve regression equation,（Coefficient correlation
R2= 0.9995）, forulic acid is 5.150 × 10-3 – 1.030×10-1Meet law of Beer in the range of mmol/L.
Measurement result of the Ultraviolet Photometric Method at 321 nm is shown in Table 1 after ferulic acid from Chinese angelica solid- liquid separation.By table 1
It can be seen that, the content of the present embodiment ferulic acid from Chinese angelica is suitable with the content of the Sichuan ferulic acid from Chinese angelica of document report.
Table 2 is the recovery testu that ferulic acid from Chinese angelica is carried out in 0.5 ︰ 1,1 ︰ 1 and 1.5 ︰ 1 ratio.Can by table 2
See, the present embodiment asafoetide acid recovering rate is 90.5-109.5%.
The announcement and teaching of book according to the above description, those skilled in the art in the invention can also enter to above-mentioned embodiment
Row change and modification.Therefore, the invention is not limited in embodiment disclosed and described above, to some of the present invention
Modifications and changes should also be as falling into the scope of the claims of the present invention.Although in addition, having used one in this specification
A little specific terms, but these terms are merely for convenience of description, do not constitute any limitation to the present invention.
Claims (9)
1. room temperature solid ionic liquid is as the forulic acid in extractant separation determination Radix Angelicae Sinensis, it is characterized in that:Synthesize a kind of room temperature
Solid ion liquid -- hexafluorophosphoric acid-N- butyl-pyridiniums（[BPy]PF6）And characterize, using it as extractant, in leaching
After forulic acid in device in leaching separation Radix Angelicae Sinensis with spectrophotometry forulic acid, extraction or be stripped at 80 DEG C
Take, be cooled to room temperature two-phase and be automatically separated.
2. [BPy] PF6 according to claim 1 synthesis, it is characterized in that：[BPy]PF6Synthesis realize in two steps, first
Step obtains [BPy] Br；Second step obtains [BPy] PF6。
3. [BPy] PF according to claim 16Sign, it is characterized in that mass spectrum, infrared spectrum determine [BPy] PF6Structure,
And determine [BPy] PF6The parameter such as fusing point, proportion.
4. leaching process apparatus according to claim 1 is characterized in：By laminated glass extractor, magnetic agitation
Device and thermostatic water-circulator bath are formed by connecting.
5. leaching according to claim 1 is characterized in the liquid-liquid extraction at 80 DEG C, when being cooled to room temperature [BPy]
PF6Automatically become solid.
6. now forulic acid is in [BPy] PF6In, extraction yield is more than 99%.
7. solid-liquid back extraction according to claim 1 is characterized in the liquid-liquid with 0.02 mol/L NaOH at 80 DEG C
Back extraction, [BPy] PF when being cooled to room temperature6Automatically become solid.
8. now forulic acid is in aqueous phase, stripping rate is more than 99%.
9. ferulic acid from Chinese angelica is determined after leaching separation according to claim 1, it is characterized in that 5.150 × 10-3
– 1.030×10-1Meet law of Beer in the range of mmol/L, asafoetide acid recovering rate is 90.5-109.5%.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110006875A (en) * | 2019-05-06 | 2019-07-12 | 黄淮学院 | Utilize the method for Raman spectroscopy biphenyl crystalline melting point |
| CN113945651A (en) * | 2021-09-26 | 2022-01-18 | 兰州石化职业技术学院 | Method for extracting ferulic acid from angelica sinensis by using non-ionic surfactant and application of ferulic acid |
| CN114018844A (en) * | 2021-10-19 | 2022-02-08 | 福建立标低碳研究院有限公司 | Method for extracting and measuring paeoniflorin in radix paeoniae rubra by pyridine ionic liquid |
-
2017
- 2017-06-30 CN CN201710525184.7A patent/CN107271391A/en not_active Withdrawn
Non-Patent Citations (1)
| Title |
|---|
| 潘仲巍 等: "室温固体离子液体作为萃取剂分离测定当归中的阿魏酸", 《第21 届全国色谱学术报告会及仪器展览会》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110006875A (en) * | 2019-05-06 | 2019-07-12 | 黄淮学院 | Utilize the method for Raman spectroscopy biphenyl crystalline melting point |
| CN110006875B (en) * | 2019-05-06 | 2021-06-01 | 黄淮学院 | Method for measuring melting point of biphenyl crystal by using Raman spectrum |
| CN113945651A (en) * | 2021-09-26 | 2022-01-18 | 兰州石化职业技术学院 | Method for extracting ferulic acid from angelica sinensis by using non-ionic surfactant and application of ferulic acid |
| CN114018844A (en) * | 2021-10-19 | 2022-02-08 | 福建立标低碳研究院有限公司 | Method for extracting and measuring paeoniflorin in radix paeoniae rubra by pyridine ionic liquid |
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Application publication date: 20171020 |