CN107271371A - A kind of planktonic organism polarization imager - Google Patents
A kind of planktonic organism polarization imager Download PDFInfo
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- CN107271371A CN107271371A CN201710635661.5A CN201710635661A CN107271371A CN 107271371 A CN107271371 A CN 107271371A CN 201710635661 A CN201710635661 A CN 201710635661A CN 107271371 A CN107271371 A CN 107271371A
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- 230000010287 polarization Effects 0.000 title claims abstract description 81
- 238000003384 imaging method Methods 0.000 claims abstract description 31
- 238000001514 detection method Methods 0.000 claims abstract description 30
- 230000010354 integration Effects 0.000 claims abstract description 23
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- 230000007797 corrosion Effects 0.000 claims 1
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- 230000008569 process Effects 0.000 abstract description 3
- 238000005259 measurement Methods 0.000 abstract description 2
- 230000005540 biological transmission Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
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- 238000011160 research Methods 0.000 description 3
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- 239000002131 composite material Substances 0.000 description 2
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- 229910000975 Carbon steel Inorganic materials 0.000 description 1
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- 229910000831 Steel Inorganic materials 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/21—Polarisation-affecting properties
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Abstract
The present invention relates to a kind of planktonic organism imager, including device housings and the Polarization Detection light path system being arranged in device housings, power-supply controller, Sample Room, sensor integration cabin and image acquisition device, wherein Polarization Detection light path system is arranged in the path channels in device housings, power-supply controller, sensor integration cabin and image acquisition device are arranged at the outside of path channels, Sample Room is arranged in sensor integration cabin, and bottom is embedded in path channels, Polarization Detection light path system is used for the planktonic organism body polarization imaging in Sample Room, the image that image acquisition device is used to capture Polarization Detection light path system is acquired and stored, it is that Polarization Detection light path system and sensor integration cabin provide power supply that power-supply controller, which is used for,.The present invention realizes the measurement of hydroplankton polarization information, effectively solves in planktonic organism image acquisition process to exist between different planktonic organisms, the image overlapping phenomenon between planktonic organism and suspended particulate.
Description
Technical field
The invention belongs to optical image technology field, specifically a kind of planktonic organism body imaging device.
Background technology
Planktonic organism is one of core of aqueous bio system production process, is important monoid in the ecosystem, is water
The basis of body food web and key components.Due to the complicated feature of planktonic organism, current planktonic organism observational study faces
Technical barrier, be the change for being difficult to quickly to measure its species composition and quantity on large-scale spatial and temporal scales.
At present, when the automatic classification for carrying out planktonic organism using image procossing and mode identification technology turns into long with counting
Between sequence halomereid study focus.Water situ imaging system is developed rapidly, it is widely used have regard under water
Frequency section plotter (Underwater Video Profiler, UVP) and planktonic organism video recorder (Video Plankton
Recorder, VPR).Both systems use optical imagery means, and the size, shape and texture to planktonic organism body are carried out
Analysis, can be achieved the different monoid of form and differentiates.Because some planktonic organism classes occur significantly in ontogenetic process
Metamorphosis, and there is the phenomenon that several different planktonic organisms are superimposed upon an image, particularly with symbiont
Image processing techniques is not yet effectively solved, and the discriminating precision of planktonic organism body awaits improving.Relative to ordinary light source
Imaging technique, polarization photoimaging can obtain the cell physiological aspect information of organism.Differentiate the monoid of planktonic organism, not only need
Differentiate its size, shape and other forms, the internal structure factor of its organism is also considered as.Therefore, to planktonic organism
Carry out polarization imaging detection, analyze its light scattering and absorption characteristic, solve that planktonic organism image information is unintelligible to cause class to reflect
Not wrong the problem of, contribute to the halomereid even research of the marine eco-environment.
The content of the invention
In view of the above-mentioned problems, it is an object of the invention to provide a kind of life of swimming based on polarized light scatter and absorption mechanism
Image objects equipment, for the collection of planktonic organism body polarization imaging information in water body, solves planktonic organism image information unclear
It is clear to cause the problem of class differentiates mistake.
To achieve these goals, the present invention uses following technical scheme：
A kind of planktonic organism polarization imager, including device housings and the Polarization Detection light that is arranged in the device housings
Path channels are provided with road system, power-supply controller, Sample Room, sensor integration cabin and image acquisition device, wherein device housings,
The Polarization Detection light path system is arranged in the path channels, and the power-supply controller, sensor integration cabin and image are adopted
Storage is arranged at the outside of the path channels, and the Sample Room is arranged in the sensor integration cabin and bottom is embedded
In in the path channels, the Polarization Detection light path system is used for the planktonic organism body polarization imaging in the Sample Room,
The image that described image collector is used to capture Polarization Detection light path system is acquired and stored, and the power-supply controller is used
In being that Polarization Detection light path system and sensor integration cabin provide power supply.
The Polarization Detection light path system include set gradually along light path single mode laser diode, pinhole filter, thoroughly
Mirror, the polarizer, water body test sample window, micro-imaging lens group, analyzer and DV, wherein water body test sample window are arranged at institute
State in Sample Room, the single mode laser diode and DV are connected with power-supply controller.
The water body test sample window is made using crystalline material.
The transmitting light direction of the single mode laser diode is vertical with the water inlet direction of water body test sample window.
The single mode laser diode provides polarized light source, and its launch wavelength should be in the sensitive volume of DV.
The central optical axis of the beam center of the single mode laser diode, the center of pinhole filter and lens are located at same
On straight line.
The photosurface of the DV is located at the focal plane of micro-imaging lens group.
The device housings are made using anticorrosion material.
Sample Room and the sensor integration cabin, which is used, is provided with what can be powered in Open architecture, the sensor integration cabin
Watertight plug, for installing water body environment parameter sensors.
The Polarization Detection light path system, power-supply controller and image acquisition device use pressure-resistant and watertight structure, circuit
Connection uses watertight connector.
The advantages of the present invention are：
1. the present invention is by using polarization imaging technology, the scattering to polarised light and absorption characteristic using planktonic organism body,
Its size, shape, texture and cell physiological aspect information are extracted, bion information in captured images is effectively ensured
Distinguish.
2. the polarized light source of the present invention, is to be sent by single mode laser diode, through pinhole filter and collimated, and adopts
The polarization state for launching light is defined with the polarizer, obtaining has high-quality polarization collimated light beam.
3. the present invention by setting micro-imaging lens group and high-resolution DV, by planktonic organism body into
As being amplified collection, clearly organism structure and texture information are obtained.
4. sensor of the invention integrated cabin is located at Sample Room both sides, and uses Open architecture, be conducive to sensor
The collection of integrated and water body environment parameter, the research of convenient auxiliary planktonic organism growing environment.
5. the polarizer and analyzer coordinate the polarization characteristic of single mode laser diode to use in the present invention, environmental background is prevented
The influence of light polarization signal.
Brief description of the drawings
Fig. 1 is the structural representation of the present invention；
Fig. 2 is the structural representation of Polarization Detection light path system in the present invention.
Wherein：1 is Polarization Detection light path system, and 2 be device housings, and 3 be power-supply controller, and 4 be Sample Room, and 5 be sensing
Device integrated cabin, 6 be image acquisition device, and 7 be single mode laser diode, and 8 be pinhole filter, and 9 be lens, and 10 be the polarizer, 11
It is micro-imaging lens group for water body test sample window, 12,13 be analyzer, and 14 be DV.
Embodiment
In order that the object, technical solutions and advantages of the present invention are clearer, below in conjunction with the accompanying drawings with specific embodiment pair
The present invention is described in detail.
As shown in figure 1, a kind of planktonic organism polarization imager for providing of the present invention, including device housings 2 and it is arranged at
Polarization Detection light path system 1, power-supply controller 3, Sample Room 4, sensor integration cabin 5 and image acquisition device 6 in standby shell 2,
Path channels are wherein provided with device housings 2, Polarization Detection light path system 1 is arranged in path channels, power-supply controller 3, biography
Sensor integrated cabin 5 and image acquisition device 6 are arranged at the outside of path channels, and Sample Room 4 is arranged in sensor integration cabin 5, simultaneously
And bottom is embedded in path channels, Polarization Detection light path system 1 is used for the planktonic organism body polarization imaging in Sample Room 4,
The image that image acquisition device 6 is used to capture Polarization Detection light path system 1 is acquired and stored, and power-supply controller 3 is for being
Polarization Detection light path system 1 and sensor integration cabin 5 provide power supply.
As shown in Fig. 2 Polarization Detection light path system 1 includes single mode laser diode 7, the pin hole filter set gradually along light path
Ripple device 8, lens 9, the polarizer 10, water body test sample window 11, micro-imaging lens group 12, analyzer 13 and DV 14, its
Reclaimed water body examination sample window 11 is arranged in Sample Room 4, and single mode laser diode 7 and DV 14 are connected with power-supply controller 3.
Water body test sample window 11 is made using crystalline material, such as uses K9 glass, quartz or sapphire light transmittance and pressure-resistant
The higher crystalline material of intensity.Water body test sample window 11 is filled in Sample Room 4, the inwall of Sample Room 4 need to be used and water body test sample window 11
Identical crystalline material, or use at water body test sample window 11 overlay structure, the i.e. light transmission part of water body test sample window 11 with this
The inwall of Sample Room 4 be same part.Do not reflected via the polarization directional light of the light transmission part of water body test sample window 11.
The transmitting light direction of single mode laser diode 7 is vertical with the water inlet direction of water body test sample window 11.The pole of single-mode laser two
Pipe 7 provides polarized light source, and its launch wavelength should be in the sensitive volume of DV 14.The light beam of single mode laser diode 7
The central optical axis at center, the center of pinhole filter 8 and lens 9 are located along the same line.
DV 14 uses high-resolution DV, and the photosurface of DV 14 is located at micro-imaging
At the focal plane of lens group 12.
Device housings 2 are made using the applied anticorrosion material in ocean, such as carbon steel lining composite polyethylene material, low-alloy sea
Water steel or titanium-based fibrous composite etc..
Power-supply controller 3 is that sensor integration cabin 5, single mode laser diode 7 and DV 14 provide different electricity
Source is exported, and the work in Synchronization Control sensor integration cabin 5, single mode laser diode 7 and high-resolution DV 14
Time.
Polarization Detection light path system 1, power-supply controller 3 and image acquisition device 6 use pressure-resistant and watertight structure, and circuit connects
Connect and use watertight connector.Sensor integration cabin 5 and Sample Room 4 use Open architecture, and can in interior be provided with sensor integration cabin 5
The watertight plug of power supply, for installing water body environment parameter sensors, including dissolved oxygen, thermohaline deep (CTD), nephelometer etc..
The operation principle of Polarization Detection light path system 1 is：
Single mode laser diode 7 launches laser beam, and via pinhole filter 8 and lens 9, it is Single wavelength that laser beam is expanded into number
Collimated light beam.The central optical axis of the beam center of single mode laser diode 7, the center of pinhole filter 8 and lens 9 exist
On same straight line.The polarizer 10 is limited the polarization state of Single wavelength collimated light beam, polarization parallel light is incided water body survey
On sample window 11, planktonic organism body is irradiated into water body.Micro-imaging lens group 12 is by the scattering and transmission of hydroplankton body
Light is amplified imaging, is conducive to obtaining the clearly demarcated planktonic organism volume morphing of structure.Micro-imaging lens group 12 and high-resolution
DV 14 constitute planktonic organism imaging optical path, the photosurface of high-resolution DV 14 is located at micro-imaging
At the focal plane of lens group 12.Water body test sample window 11 is less than planktonic organism in boundary's distance between the surface on water circulating direction
The depth of field of imaging optical path, it is ensured that clearly planktonic organism body is imaged.The specific depth of field of planktonic organism imaging optical path should be met：
In formula, Δ L1For the preceding depth of field, Δ L2For the rear depth of field, ∫ is imaging optical path focal length, and F is the effective f-number of imaging optical path
Value, δ is allows disperse circular diameter, and L is focal distance.
Analyzer 13 is by the way of horizontal direction polarization and vertical direction polarization conversion, high-resolution DV
14 obtain the planktonic organism body image of different polarization degree.Specific degree of polarization should be met：
Total degree of polarization：
Linear polarization degree：
Circular polarization：
In formula, I is horizontal state of polarization component and polarization and vertical polarization component sum, i.e. total light intensity；Q is horizontal state of polarization component
With the difference of polarization and vertical polarization component；U is the difference of 45 ° of polarization state components and -45 ° of polarization state components；V be dextropolarization state component with
The difference of left-hand polarization state component.
In summary, power-supply controller 3 controls the startup and closing of planktonic organism polarization imager.In planktonic organism polarization
After the startup of imager, the transmitting laser beam of single mode laser diode 7, via pinhole filter 8, lens 9 and the polarizer 10, will swash
Light beam expands the polarization collimated light beam that number is Single wavelength, the scattering being irradiated in water body on planktonic organism body and transmitted light, via aobvious
After micro- imaging lens group 12 and analyzer 13, received by high-resolution DV 14, its imaging is entered by image acquisition device 6
Row collection and storage.Water during the equipment synchronous acquisition planktonic organism polarization imager work installed in sensor integration cabin 5
Body ambient parameter.
The automatic classification of planktonic organism provides a kind of polarization imaging detection side during the present invention studies for halomereid
Formula, its one side obtains size, shape and the texture information of planktonic organism body, on the other hand obtains inclined inside planktonic organism body
Shake light scattering and light transmission features, information in terms of the cell physiological to extract organism.Present invention can apply in Different Waters
Mobile or fixation measuring platform, can also be used alone, realize the measurement of hydroplankton polarization information, can be right
The feature such as the distribution of planktonic organism, abundance, form and internal structure is measured in water body, effectively solves planktonic organism image
Exist in gatherer process between different planktonic organisms, the image overlapping phenomenon between planktonic organism and suspended particulate, be planktonic organism point
Class provides the scattered information of organism internal structure.
The present invention obtains the internal structure factor of planktonic organism body by polarization imaging mode, with reference to its size, shape with
And other forms, the problem of planktonic organism image information is unintelligible to cause class discriminating mistake is solved, is contributed to water ecology
Environment and the research of planktonic organism.
Embodiments of the present invention are the foregoing is only, are not intended to limit the scope of the present invention.It is all in the present invention
Spirit and principle within any modification, equivalent substitution and improvements, the extension made etc., be all contained in protection scope of the present invention
It is interior.
Claims (10)
1. a kind of planktonic organism polarization imager, it is characterised in that including device housings (2) and be arranged at the device housings
(2) Polarization Detection light path system (1), power-supply controller (3), Sample Room (4), sensor integration cabin (5) and IMAQ in
Path channels are provided with device (6), wherein device housings (2), the Polarization Detection light path system (1) is arranged at the path channels
Interior, the power-supply controller (3), sensor integration cabin (5) and image acquisition device (6) are arranged at the outside of the path channels,
The Sample Room (4) is arranged in the sensor integration cabin (5) and bottom is embedded in the path channels, described inclined
The detection light path system (1) that shakes is used for the planktonic organism body polarization imaging in the Sample Room (4), described image collector (6)
Image for Polarization Detection light path system (1) to be captured is acquired and stored, and the power-supply controller (3) is used for for polarization
Detect that light path system (1) and sensor integration cabin (5) provides power supply.
2. planktonic organism polarization imager according to claim 1, it is characterised in that the Polarization Detection light path system
(1) single mode laser diode (7), pinhole filter (8), lens (9), the polarizer (10), the water set gradually along light path is included
Body examination sample window (11), micro-imaging lens group (12), analyzer (13) and DV (14), wherein water body test sample window (11)
It is arranged in the Sample Room (4), the single mode laser diode (7) and DV (14) connect with power-supply controller (3)
Connect.
3. planktonic organism polarization imager according to claim 2, it is characterised in that the water body test sample window (11) uses
Crystalline material makes.
4. planktonic organism polarization imager according to claim 2, it is characterised in that the single mode laser diode (7)
Transmitting light direction it is vertical with the water inlet direction of water body test sample window (11).
5. planktonic organism polarization imager according to claim 2, it is characterised in that the single mode laser diode (7)
Polarized light source is provided, its launch wavelength should be in the sensitive volume of DV (14).
6. planktonic organism polarization imager according to claim 2, it is characterised in that the single mode laser diode (7)
Beam center, the central optical axis of the center of pinhole filter (8) and lens (9) are located along the same line.
7. planktonic organism polarization imager according to claim 2, it is characterised in that the DV 14 it is photosensitive
Face is located at the focal plane of micro-imaging lens group (12).
8. planktonic organism polarization imager according to claim 1, it is characterised in that the device housings (2) are using anti-
Corrosion material makes.
9. planktonic organism polarization imager according to claim 1, it is characterised in that the Sample Room (4) and sensor
Integrated cabin (5) is used provided with the watertight plug that can be powered in Open architecture, the sensor integration cabin (5), for installing
Water body environment parameter sensors.
10. planktonic organism polarization imager according to claim 1, it is characterised in that the Polarization Detection light path system
(1), power-supply controller (3) and image acquisition device (6) use pressure-resistant and watertight structure, and circuit connection uses watertight connector.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710635661.5A CN107271371A (en) | 2017-07-28 | 2017-07-28 | A kind of planktonic organism polarization imager |
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| CN201710635661.5A CN107271371A (en) | 2017-07-28 | 2017-07-28 | A kind of planktonic organism polarization imager |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108088829A (en) * | 2017-12-28 | 2018-05-29 | 湖南华南光电科技股份有限公司 | Consumptive material sealing structure based on fluorescent quenching technology trace explosive detector |
| CN108810391A (en) * | 2018-09-07 | 2018-11-13 | 百度在线网络技术（北京）有限公司 | Photographic device |
| CN110057824A (en) * | 2019-05-06 | 2019-07-26 | 中国科学院海洋研究所 | A kind of halomereid optical imaging device and image processing method |
| CN111292537A (en) * | 2018-12-06 | 2020-06-16 | 中国科学院长春光学精密机械与物理研究所 | Night license plate recognition system and method |
| CN114674760A (en) * | 2022-05-30 | 2022-06-28 | 中国科学院海洋研究所 | Symmetric marine planktonic algae polarization scattering multi-angle measuring instrument and measuring method thereof |
| CN115184276A (en) * | 2022-08-09 | 2022-10-14 | 中国科学院海洋研究所 | Towed plankton polarization imaging recorder |
| CN115656175A (en) * | 2022-12-09 | 2023-01-31 | 海南浙江大学研究院 | Plankton monitoring system for backlight imaging and data processing method |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108088829A (en) * | 2017-12-28 | 2018-05-29 | 湖南华南光电科技股份有限公司 | Consumptive material sealing structure based on fluorescent quenching technology trace explosive detector |
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| CN108810391A (en) * | 2018-09-07 | 2018-11-13 | 百度在线网络技术（北京）有限公司 | Photographic device |
| CN111292537A (en) * | 2018-12-06 | 2020-06-16 | 中国科学院长春光学精密机械与物理研究所 | Night license plate recognition system and method |
| CN111292537B (en) * | 2018-12-06 | 2023-05-16 | 中国科学院长春光学精密机械与物理研究所 | System and method for identifying license plate at night |
| CN110057824A (en) * | 2019-05-06 | 2019-07-26 | 中国科学院海洋研究所 | A kind of halomereid optical imaging device and image processing method |
| CN110057824B (en) * | 2019-05-06 | 2024-05-14 | 中国科学院海洋研究所 | Ocean plankton optical imaging device and imaging processing method |
| CN114674760A (en) * | 2022-05-30 | 2022-06-28 | 中国科学院海洋研究所 | Symmetric marine planktonic algae polarization scattering multi-angle measuring instrument and measuring method thereof |
| CN115184276A (en) * | 2022-08-09 | 2022-10-14 | 中国科学院海洋研究所 | Towed plankton polarization imaging recorder |
| CN115184276B (en) * | 2022-08-09 | 2023-09-26 | 中国科学院海洋研究所 | Towed plankton polarization imaging recorder |
| CN115656175A (en) * | 2022-12-09 | 2023-01-31 | 海南浙江大学研究院 | Plankton monitoring system for backlight imaging and data processing method |
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Application publication date: 20171020 |